WADA Technical Letter – TL07 ANDARINE

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WADA Technical Letter – TL07

Document Number: TL07
Version Number:
3.0

Written by:

WADA Science

Approved by:
WADA Executive Committee

Reviewed by: Date:
WADA Laboratory Expert Group

21 December 2020
Effective Date:
1 January 2021

ANDARINE - FLUTAMIDE
1.0 Introduction

WADA wishes to draw the attention of the Laboratories to the following observations and instructions on

the analysis and reporting of SARM S4 (Andarine) Metabolites O-dephenylandarine and O- dephenylandarine glucuronide.

The andarine Metabolites O-dephenylandarine and of O-dephenylandarine glucuronide may also be present in a Sample as a Metabolite of the anti-androgen Flutamide (Drogenil^®, Eulexin^®, Euflex^®, Flutamin^®, Flugere^®), used primarily in the treatment of prostate cancer ^[1]. Since flutamide is not a

Prohibited Substance in sports, the Laboratories shall not report an Adverse Analytical Finding (AAF) for

andarine based on the monitoring of O-dephenylandarine ^[2].

Figure 1. Proposed metabolic pathway of flutamide and andarine (adapted from Perrenoud et al. ^[1]).

2.0 Analysis and Reporting Requirements

Report the result as an AAF for andarine only when the presence of andarine (parent compound), and/or its glucuronic acid conjugate, and/or its deacetylated and/or hydroxylated Metabolites, and/or its

[2]

bishydroxylated product are confirmed in the Sample (regardless of the presence of flutamide and/or its Metabolite 2-hydroxyflutamide);

[Comment: Laboratories shall not report an AAF for andarine based only on the presence of O- dephenylandarine.]

3.0 References

[1] Perrenoud L. et al. Risk of false positive results to SARM S4 in case of therapeutic use of antineoplastic/antiandrogen drug containing flutamide: a case study. Drug Test Anal. 8(11-12): 1109-13, 2016.

[2] Thevis M. et al. Mass spectrometric characterization of urinary metabolites of the selective androgen receptor modulator andarine (S‐4) for routine doping control purposes. Rapid Commun Mass Sp. 24(15): 2245-54, 2010.

TL07 – Version 3.0
Page 1 of 1

Recommended publications

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(12) United States Patent (10) Patent No.: US 8,853,266 B2 Dalton Et Al

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Development and Validation of a Semi-Quantitative Ultra-High

G Model CHROMA-360196; No. of Pages 14 ARTICLE IN PRESS Journal of Chromatography A, xxx (2019) xxx–xxx Contents lists available at ScienceDirect Journal of Chromatography A j ournal homepage: www.elsevier.com/locate/chroma Development and validation of a semi-quantitative ultra-high performance liquid chromatography-tandem mass spectrometry method for screening of selective androgen receptor modulators in urine a,∗ a,∗ a a b,c Emiliano Ventura , Anna Gadaj , Gail Monteith , Alexis Ripoche , Jim Healy , d e f a Francesco Botrè , Saskia S. Sterk , Tom Buckley , Mark H. Mooney a Institute for Global Food Security, School of Biological Sciences, Queen’s University Belfast, BT9 5AG, United Kingdom b Laboratory, Irish Greyhound Board, Limerick Greyhound Stadium, Ireland c Applied Science Department, Limerick Institute of Technology, Moylish, Limerick, Ireland d Laboratorio Antidoping, Federazione Medico Sportiva Italiana, Italy e RIKILT Wageningen University & Research, European Union Reference Laboratory, Wageningen, the Netherlands f Irish Diagnostic Laboratory Services Ltd., Johnstown, Co. Kildare, Ireland a r t i c l e i n f o a b s t r a c t Article history: A semi-quantitative method was developed to monitor the misuse of 15 SARM compounds belonging Received 25 January 2019 to nine different families, in urine matrices from a range of species (equine, canine, human, bovine and Received in revised form 16 April 2019 murine). SARM residues were extracted from urine (200 ␮L) with tert-butyl methyl ether (TBME) without Accepted 17 April 2019 further clean-up and analysed by ultra-high performance liquid chromatography coupled to tandem mass Available online xxx spectrometry (UHPLC-MS/MS).
Anabolic Agents: Recent Strategies for Their Detection and Protection from Inadvertent Doping Hans Geyer,1,2 Wilhelm Schänzer,1 Mario Thevis1,2

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Prohibited List 2021

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