Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

15-Hydroxy Lubiprostone Human plasma 1-150 pgmL EDTA 15-Hydroxy Lubiprostone 35-7000 pg/mL Rat plasma EDTA Lubiprostone 35-7000 pg/mL 17-Desacetyl- Human plasma 50-5000 pg/mL EDTA 25-Hydroxy-vitamin D2 (Calciferol) Human plasma 5-250 ng/mL EDTA (DEQAS certified) 25-Hydroxy-vitamin D3 (Calcifediol) Human plasma 5-250 ng/mL EDTA (DEQAS certified) 3-Ketodesogestrel Human plasma 0.05-10 ng/mL EDTA

Acetaminophen Human plasma 200-10000 ng/mL EDTA

Acetazolamine Human plasma 50-20000 ng/mL EDTA

Acetylsalicylic Acid Human plasma 5-2000 ng/mL Fluoride/Oxalate 20-8000 ng/mL Acetylsalicylic Acid, and Human plasma 80-32000 ng/mL Fluoride/Oxalate Butalbital 10-4000 ng/mL Afatinib Human plasma 0.1-50 ng/mL EDTA

Albuterol Human plasma 0.25-50 ng/mL EDTA

Page 1 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Alendronate Human 1-200 ng/mL N/A

Alfuzosin Human plasma 0.15-30 ng/mL EDTA

Aliskiren Human plasma 1-500 ng/mL EDTA

Alprazolam Human plasma 0.25-25 ng/mL EDTA

Amlodipine Human plasma 0.1-15 ng/mL EDTA

Anagrelide Human plasma 0.05-11 ng/mL EDTA 0.5-150 ng/mL D,L-Amphetamine Human plasma EDTA 0.25-30 ng/mL Anastrazole Human plasma 0.1-20 ng/mL EDTA

Aripiprazole Human plasma 0.5-100 ng/mL EDTA

Asenapine Human plasma 0.05-20 ng/mL EDTA

Asoxime Human plasma 0.1-30 µg/mL EDTA

Asoxime Human urine 1-300 µg/mL N/A

Page 2 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Atomoxetine Human plasma 2-2000 ng/mL EDTA 0.15-150 ng/mL Atorvastatin, O-Hydroxy-Atorvastatin and Human plasma 0.15-150 ng/mL EDTA P-Hydroxy-Atorvastatin) 0.1-15 ng/mL 0.02-20 ng/mL Atorvastatin, O-Hydroxy-Atorvastatin and Human plasma 0.02-20 ng/mL EDTA P-Hydroxy-Atorvastatin 0.01-1.5 ng/mL Atorvastatin Human plasma 0.25-200 ng/mL EDTA

Atovaquone Human plasma 50-25000 ng/mL EDTA 5-1000 ng/mL Benazepril and Benazeprilat Human plasma EDTA 7.5-1500 ng/mL 0.5-200 ng/mL Bexarotene Human plasma EDTA 1-500 ng/mL Betamethasone Human plasma 0.5-250 ng/mL Heparin

Bezafibrate Human plasma 15-5000 ng/mL EDTA

Bicalutamide Human plasma 10-1000 ng/mL EDTA Chiral S and R form 4-300 ng/mL Human plasma EDTA 16-1200 ng/mL

Page 3 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Bisoprolol Human plasma 1-100 ng/mL EDTA 0.04-8 ng/mL and Norbuprenorphine Human plasma EDTA 0.02-4 ng/mL 2.5-500 ng/mL , , Erythro- 12.5-2500 ng/mL Hydroxybupropion and Threo- Human plasma EDTA 4-800 ng/mL Hydroxybupropion 8-1600 ng/mL 1-200 ng/mL Buproprion and hydroxybupropion Human plasma EDTA 4-800 ng/mL Calcifediol Human plasma 5-250 ng/mL EDTA

Calcitriol Human plasma 10-200 pg/mL EDTA Carbamazepine and Carbamazepine 10,11- 25-5000 ng/mL Human plasma EDTA epoxide 1.25-250 ng/mL 1-250 ng/mL Carbidopa and Levodopa Human plasma 8-2000 ng/mL EDTA 2-400 ng/mL Carbidopa and Levodopa Human plasma Fluoride/Oxalate 10-2000 ng/mL 0.5-200 ng/mL Carbidopa, Levodopa and Etilevodopa Human plasma 6.25-2500 ng/mL Fluoride/Oxalate 0.05-20 ng/mL

Page 4 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant 10-2500 ng/mL Carisoprodol and Meprobamate Human plasma EDTA 10-2500 ng/mL 5-2500 ng/mL Carisoprodol and Meprobamate Dog plasma EDTA 5-2500 ng/mL 0.5-100 ng/mL Carvedilol and 4-Hydroxyphenyl Carvedilol Human plasma EDTA 0.1-10 ng/mL 0.25-50 ng/mL Carvedilol and 4-Hydroxyphenyl Carvedilol Human plasma EDTA 0.05-10 ng/mL Cellulose butyrate Mini Pig plasma 20-200 µg/mL EDTA

Cellulose acetate butyrate Rat plasma 20-200 µg/mL EDTA Chlorambucil and Phenylacetic Acid 5-1600 ng/mL Human serum N/A Mustard 5-1600 ng/mL 0.1-15 ng/mL Cholecalciferol (Vitamin D3), Calcitriol and Human serum 0.01-1.5 ng/mL N/A Calcifediol (25-hydroxy-vitamin D3) 2-300 ng/mL

Cholecalciferol (Vitamin D3) Human plasma 0.1-30 ng/mL EDTA 50-8000 ng/mL Ciprofloxacin and Gatifloxacin Human plasma EDTA 50-8000 ng/mL Ciprofloxacin Human plasma 30-6000 ng/mL EDTA

Page 5 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Clarithromycin Human plasma 15-6000 ng/mL EDTA

Clarithromycin Human plasma 10-2000 ng/mL EDTA

Clonidine Human plasma 1-200 pg/mL EDTA

Clonidine Human plasma 10-2000 pg/mL EDTA

Clonidine Rabbit plasma 10-2000 pg/mL EDTA 0.5-125 ng/mL and Caffeine Human plasma EDTA 10-2500 ng/mL Cyclobenzaprine Human plasma 0.05-20 ng/mL EDTA

Cyclobenzaprine Human plasma 0.1-40 ng/mL EDTA

Cyproterone Acetate Human plasma 0.9-180 ng/mL EDTA

Cyproterone Acetate Human plasma 0.25-50 ng/mL EDTA

Danazol Human plasma 0.8-200 ng/mL EDTA

Darifenacin Human plasma 0.05-10 ng/mL EDTA

Page 6 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Dasatinib Human plasma 0.5-200 ng/mL EDTA 25-6250 pg/mL Desloratadine and 3-Hydroxy-Desloratadine Human plasma EDTA 12-3000 pg/mL Dexmethylphenidate Human plasma 0.15-45 ng/mL EDTA

Dexmethylphenidate Human plasma 0.1-30 ng/mL EDTA

Diclofenac Human plasma 8-4000 ng/mL EDTA

Didanosine Human plasma 10-1500 ng/mL EDTA

Dienogest Human plasma 0.5-150 ng/mL EDTA 1.5-500 ng/mL Diltiazem, N-Desmethyl Diltiazem and Human plasma 0.3-100 ng/mL EDTA Desacetyl Diltiazem 0.15-50 ng/mL Dipyridamole Human plasma 10-4000 ng/mL Fluoride/Oxalate

Donepezil Human plasma 0.1-20 ng/mL EDTA 0.5-50 ng/mL Donepezil and Human plasma EDTA 0.5-50 ng/mL Doxazosin Human plasma 0.5-125 ng/mL EDTA

Page 7 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Doxercalciferol and Ercalcitriol Human serum 2-80 pg/mL N/A 2-80 pg/mL Doxercalcifero and Ercalcitriol Human plasma EDTA 2-80 pg/mL Doxycycline Human plasma 5-1000 ng/mL EDTA 0.5-150 ng/mL Doxylamine and Pyridoxine Human plasma EDTA 0.25-100 ng/mL Human plasma 0.5-150 ng/mL EDTA

Drospirenone Human plasma 0.075-30 ng/mL EDTA

Elesclomol and Metabolites Human plasma 1-1000 ng/mL Heparin 0.5-125 ng/mL Enalapril and Enalaprilat Human plasma EDTA 0.25-62.5 ng/mL Entacapone Human Plasma 25-2500 ng/mL EDTA

Enzalutamide Human Plasma 20-8000 ng/mL EDTA 0.5-250 ng/mL EPA ethyl ester and DHA ethyl ester Human plasma EDTA 0.5-250 ng/mL 1.6 -120 µg/mL EPA Total and DHA Total Human plasma EDTA 8-200 µg/mL

Page 8 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

EPA ethyl ester Human plasma 1-1000 ng/mL EDTA

Esomeprazole Human plasma 4-2000 ng/mL EDTA

Ethinyl Human plasma 2-500 pg/mL EDTA

Estradiol Free Human plasma 1-150 pg/mL EDTA

Estradiol Total Human serum 2-400 pg/mL N/A 4-300 pg/mL Free and Equilin Free Human plasma EDTA 2-150 pg/mL 10-1000 pg/mL Estrone Free and Estradiol Free Human plasma EDTA 2-200 pg/mL 5-1000 pg/mL Estrone Free, Equilin Free and Delta 8,9- Human plasma 2-400 pg/mL EDTA Dehydroestrone 2-400 pg/mL 4-300 pg/mL Estrone Free, Estradiol Free, Equilin Free 2-150 pg/mL Human plasma EDTA and Dihydroequilin Free 2-150 pg/mL 2-150 pg/mL Human plasma 50-10000 pg/mL EDTA Estrone Total and Equilin Total 50-10000 pg/mL

Page 9 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant 50-10000 pg/mL Estrone Total, Equilin Total and Delta 8,9- Rabbit plasma 50-10000 pg/mL EDTA Dehydroestrone 15-3000 pg/mL Estrone Total Human serum 0.15-75 ng/mL N/A

Ethoxutimide Human plasma 0.1-10 ng/mL EDTA

Etidronate Human plasma 10-2000 ng/mL Heparin

Ezetimibe Free Human plasma 0.05-10 ng/mL EDTA

Ezetimibe Total Human plasma 0.5-150 ng/mL EDTA

Felodipine Human plasma 10-5000 pg/mL EDTA

Fenofibric Acid Rat plasma 10-10000 ng/mL EDTA

Fenofibric Acid Human plasma 50-15000 ng/mL EDTA

Fentanyl Human plasma 0.01-1.5 ng/mL EDTA 1-400 ng/mL Fexofenadine and Pseudoephedrine Human plasma EDTA 1.5-600 ng/mL 0.05-10 ng/mL Fidaxomicin and Desisobutyryl Fidaxomicin Human plasma Fluoride/Oxalate 0.05-20 ng/mL

Page 10 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Finasteride Human plasma 0.5-50 ng/mL EDTA

Finasteride Human plasma 0.1-10 ng/mL EDTA

Fluticasone Propionate Human plasma 1-200 pg/mL Fluoride/Oxalate

Fosinoprilat Human plasma 5-750 ng/mL EDTA

Gabapentin Human plasma 50-10000 ng/mL EDTA

Galanthamine Human plasma 0.1-30 ng/mL EDTA 70-12600 ng/mL Ganciclovir and Valganciclovir Human plasma EDTA 5-900 ng/mL 50-8000 ng/mL Gatifloxacin and Ciprofloxacin Human plasma EDTA 50-8000 ng/mL Human plasma 1-500 ng/mL EDTA

Glipizide Human plasma 10-1500 ng/mL EDTA

Glyburide Human plasma 2.5-300 ng/mL EDTA

Guanfacine Human plasma 40-8000 pg/mL EDTA

Page 11 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Guaifenesin Human plasma 30-6000 ng/mL EDTA

Hydrochlorothiazide Human plasma 1.5 -250 ng/mL EDTA

Hydrochlorothiazide Human plasma 3-500 ng/mL EDTA

Hydrocodone Human plasma 0.2-50 ng/mL EDTA

Hydrocortisone Human plasma 10-1000 ng/mL EDTA

Hydromorphone Human plasma 20-6000 pg/mL EDTA

Ibuprofen Human plasma 0.2-40 µg/mL EDTA 5-2000 ng/mL Iacvita and Tempol Rat plasma EDTA 5-2000 ng/mL Irbesartan Human plasma 30-6000 ng/mL EDTA

Isradipine Human plasma 0.05-10 ng/mL EDTA 1-400 ng/mL Itraconazole and Hydroxy-Itraconazole Human plasma EDTA 1.25-500 ng/mL 0.1-20 ng/mL Ivabradine and N-Desmethyl Ivabradine Human plasma EDTA 0.05-10 ng/mL

Page 12 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant 0.5-500 ng/mL Chiral and Human plasma EDTA 0.5-500 ng/mL 0.5-500 ng/mL Chiral Ketamine, Norketamine and Dog plasma 0.5-500 ng/mL EDTA Dehydronorketamine 0.5-500 ng/mL Racemic Human plasma 50-10000 ng/mL EDTA

Lansoprazole Human plasma 3-3000 ng/mL EDTA

Letrozole Human plasma 1-200 ng/mL EDTA

Levetiracetam Human plasma 0.25-50 µg/mL EDTA

Levofloxacin Rabbit plasma 0.1-12 ng/mL Heparin

Levomefolate Chiral Human plasma 1-100 ng/mL EDTA

Levonorgestrel Human plasma 0.15-15 ng/mL EDTA

Levonorgestrel Human plasma 0.25-40 ng/mL EDTA

Levonorgestrel Human plasma 0.08-5 ng/mL EDTA 0.25-100 ng/mL Lisdexamfetamine and D-Amphetamine Human plasma EDTA 1.25-125 ng/mL

Page 13 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Lisinopril Human plasma 1-200 ng/mL EDTA 0.04-10 ng/mL Loratadine and Descarboethoxyloratadine Human plasma EDTA 0.04-10 ng/mL Lorazepam Human plasma 0.3-45 ng/mL EDTA

Losartan Human plasma 6-1200 ng/mL EDTA

Medroxyprogesterone 17-Acetate Human plasma 0.025-10 ng/mL EDTA

Meloxicam Dog plasma 5-1000 ng/mL EDTA

Meloxicam Human plasma 10-2000 ng/mL EDTA 0.5-50 ng/mL Memantine and Donepezil Human plasma EDTA 0.5-50 ng/mL 1-1500 ng/mL Mesalamine and N-acethylmesalamine Human plasma EDTA 1-1500 ng/mL Mesalamine Human plasma 14-3500 ng/mL EDTA

Mesalamine Human plasma 7-1750 ng/mL EDTA

Metformin Human plasma 25-3000 ng/mL EDTA

Page 14 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant 25-3000 ng/mL Metformin and Sitagliptin Human plasma EDTA 5-1000 ng/mL Methylphenidate Human plasma 0.05-15 ng/mL EDTA

Methylphenidate Human plasma 0.1-30 ng/mL EDTA 5-1500 ng/mL Metopimazine and Metopimazine Acid Human plasma EDTA 10-3000 ng/mL 1-2000 ng/mL Metopimazine and Metopimazine Acid Human urine N/A 10-60000 ng/mL 1-300 ng/mL Metopimazine , Metopimazine Acid and Human plasma 5-1500 ng/mL EDTA Metopimazine Sulfoxide 1-300 ng/mL Metoprolol Human plasma 0.5-500 ng/mL EDTA

Metoprolol Human plasma 0.1-25 ng/mL EDTA 0.05-50 ng/mL Midazolam and 1’-Hydroxymidazolam Human plasma EDTA 0.05-25 ng/mL Minocycline Human plasma 10-1000 ng/mL EDTA

Mirtazepine Human plasma 0.1-60 ng/mL EDTA

Misoprostol Acid Human plasma 10-1250 pg/mL EDTA

Page 15 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Misoprostol Acid Human plasma 2-800 pg/mL EDTA

Monomethyl Fumarate Human plasma 20-4000 ng/mL Fluoride/Oxalate 0.8-80 ng/mL and Morphine-6β-D-Glucuronide Human plasma EDTA 4-400 ng/mL 0.5-50 ng/mL Morphine and Morphine-6β-D-Glucuronide Human plasma EDTA 2.5-250 ng/mL 0.1-30 ng/mL Morphine , Morphine-6β-D-Glucuronide and Human plasma 0.5-150 ng/mL EDTA 0.1-30 ng/mL Moxifloxacin Human plasma 25-6000 ng/mL EDTA

Mycophenolate Mofetil Human plasma 20-4000 pg/mL Fluoride/Oxalate

Mycophenolic Acid Human plasma 100-20000 ng/mL Fluoride/Oxalate 1-1000 pg/mL and 6-β-Naltrexol Human plasma EDTA 0.5-500 pg/mL Human plasma 1.5-150 µg/mL EDTA

Naproxen Human plasma 1-100 µg/mL EDTA

Naratriptan Human serum 0.075-15 ng/mL N/A

Page 16 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant 1-500 ng/mL Nefopam and N-Desmethylnefopam Human plasma 1-500 ng/mL EDTA

1-500 ng/mL Nefopam, N-Desmethylnefopam and Human plasma 1-500 ng/mL EDTA N-Oxidenefopam 1-500 ng/mL 1-500 ng/mL Nefopam, N-Desmethylnefopam and Human urine 1-500 ng/mL N/A N-Oxidenefopam 1-500 ng/mL 10-6000 ng/mL Niacin and Nicotinuric Acid Human plasma EDTA 10-3000 ng/mL Human plasma 1-50 ng/mL EDTA

Nicotine Human plasma 0.2-15 ng/mL EDTA

Nomegestrol Acetate Human plasma 0.1-15 ng/mL Fluoride/Oxalate

Norethindrone Human plasma 0.2-25 ng/mL EDTA

Norethindrone Human plasma 0.4-50 ng/mL EDTA

Norgestrel Human plasma 0.08-10 ng/mL EDTA

Norgestrel Human plasma 0.15-15 ng/mL EDTA

Page 17 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Olanzapine Human plasma 0.1-15 ng/mL Heparin

Omeprazole Human plasma 1-1000 ng/mL EDTA 0.5-100 ng/mL Oseltamivir and Oseltamivir Carboxylic Acid Human plasma Fluoride/Oxalate 2-400 ng/mL Oxcarbazepine and 10,11 Dihydro-10- 20-6000 ng/mL Human plasma EDTA Dihydrooxcarbazepine 50-15000 ng/mL Oxybutynin Dog plasma 0.2-10 ng/mL EDTA 1-500 ng/mL Oxybutynin and Desethyloxybutynin Dog plasma EDTA 1-500 ng/mL Oxycodone Human plasma 0.3-75 ng/mL EDTA and 20-5000 pg/mL Human plasma EDTA 6β-Hydroxyoxymorphone 10-2500 pg/mL Paracalcitol Human plasma 10-1000 pg/mL EDTA

Paracalcitol Human plasma 5-500 pg/mL EDTA

Paroxetine Human plasma 0.1-60 ng/mL EDTA

Pazopanib Human plasma 0.5-100 µg/mL EDTA

Page 18 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Phentermine Human plasma 1-200 ng/mL EDTA

Posaconazole Human plasma 2-1000 ng/mL Fluoride/Oxalate 40-2000 pg/mL Pramlintide and DesLyspramlintide Human plasma EDTA 40-2000 pg/mL Pravastatin Human plasma 0.5-500 ng/mL EDTA

Praziquantel Human plasma 5-1500 ng/mL EDTA

Progesterone Human plasma 0.1-150 ng/mL EDTA

Progesterone Human plasma 0.05-30 ng/mL EDTA

Progesterone Dog plasma 0.2-300 ng/mL EDTA 0.2-300 ng/mL Propafenone and 5-Hydroxy Propafenone Human plasma EDTA 0.1-150 ng/mL Propranolol Free Human plasma 1-500 ng/mL EDTA 2-500 ng/mL Propranolol Total and 4-OH Propanolol Human plasma EDTA 0.5-150 ng/mL Propoxyphene Human plasma 0.25-100 ng/mL EDTA

Page 19 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant Pseudoephidrine Human plasma 1.5-600 ng/mL EDTA

Pyridostigmine Human plasma 1-200 ng/mL EDTA 0.5-200 ng/mL Pyridoxal and Pyridoxal-5-Phosphate Human plasma EDTA 2.5-200 ng/mL Quetiapine Human Plasma 4-1000 ng/mL EDTA

Rabeprazole Human Plasma 1-500 ng/mL EDTA 5-1250 pg/mL Raloxifene, Raloxifene-4-Glucuronide and Human plasma 2-500 ng/mL EDTA Raloxifene-6-Glucuronide 0.3-75 ng/mL Ramipril Human plasma 0.2-80 ng/mL EDTA

Ranolazine Human plasma 10-4000 ng/mL EDTA 0.015-10 ng/mL Rasagiline and 1-Aminoindan Human plasma EDTA 0.125-10 ng/mL Rasagiline Dog plasma 0.2-500 ng/mL EDTA

Repaglinide Human plasma 0.2-60 ng/mL EDTA

Risedronic acid Human plasma 0.2-150 ng/mL EDTA

Page 20 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Risperidone Human plasma 0.05-10 ng/mL EDTA

Rivastigmine Human plasma 0.2-60 ng/mL Fluoride/Oxalate

Rivastigmine Human plasma 0.02-6 ng/mL Fluoride/Oxalate

Ropinirole Human plasma 10-2000 pg/mL EDTA

Rosiglitazone Human plasma 2-900 ng/mL EDTA

Rotigotine Human plasma 2-400 pg/mL EDTA

Sertraline Human plasma 0.25-75 ng/mL EDTA

Scopolamine Human plasma 1-300 pg/mL Fluoride/Oxalate 0.25-100 ng/mL Sirolimus and Tacrolimus Human blood EDTA 0.1-40 ng/mL 0.1-50 ng/mL Simvastatin and Simvastatin Hydroxy Acid Human plasma EDTA 0.1-20 ng/mL Sotalol Human plasma 20-2000 ng/mL EDTA

Spironolactone Human Plasma 0.5-150 ng/mL Fluoride/Oxalate

Page 21 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant

Sumatriptan Human plasma 0.5-100 ng/mL EDTA

Tadalafil Human plasma 4-600 ng/mL EDTA

Tamsulosin Human plasma 0.25-50 ng/mL EDTA

Tapentadol Human plasma 0.5-200 ng/mL EDTA

Telmisartan Human plasma 4-1000 ng/mL EDTA

Terbinafine Human plasma 1-2000 ng/mL EDTA

Theophylline Human plasma 2-500 ng/mL Fluoride/Oxalate

Tofacitinib Human plasma 0.5-100 ng/mL EDTA

Topiramate Human plasma 25-2500 ng/mL EDTA

Topiramate Human plasma 5-500 ng/mL EDTA 2-1000 ng/mL and O- Desmethyl-Tramadol Pig serum N/A 1-500 ng/mL 1-200 ng/mL Tramadol and O-Desmethyl-Tramadol Human plasma EDTA 0.2-40 ng/mL

Page 22 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant 10-1000 ng/mL Tramadol and O-Desmethyl-Tramadol Human plasma EDTA 1.25-250 ng/mL Trientine and N1-Acetyl 4-600 ng/mL Human plasma EDTA Triethylenetetramine 4-600 ng/mL 1-400 ng/mL and N-Desmethyl Trimebutine Human plasma EDTA 12.5-5000 ng/mL Trimetazidine Dog plasma 0.075-200 µg/mL EDTA

Trimetazidine Human plasma 10-1000 ng/mL EDTA

Trimetazidine Human urine 0.2-200 µg/mL N/A

Trimetazidine Rat plasma 0.1-200 µg/mL EDTA

Thiostrepton Human plasma 1-2000 ng/mL EDTA

Thiostrepton Human pleural fluid 1-2000 ng/mL N/A

Valproic Acid Human plasma 1-200 µg/mL EDTA

Valsartan Human plasma 10-2500 ng/mL EDTA 2.5-750 ng/mL Venlafaxine and O-Desmethyl Venlafaxine Human plasma EDTA 2.5-750 ng/mL

Page 23 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Compound (Metabolite) Species/Matrix Range Anticoagulant 1-300 ng/mL Verapamil and Norverapamil Human plasma EDTA 1-300 ng/mL Voriconazole Human plasma 10-2500 ng/mL EDTA

Ziprasidone Human serum 0.4-80 ng/mL N/A

Zolmitriptan Human plasma 40-8000 pg/mL EDTA 150-30000 ng/mL Zolmitriptan and N-Desmethylzolmitriptan Rat plasma EDTA 15-3000 ng/mL Zolpidem Human plasma 1-250 ng/mL EDTA

Zolpidem Human plasma 0.25-62.5 ng/mL EDTA

Page 24 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Qualified Bioanalytical Assays and Under Development

Qualified Under Species/Matrix Range Anticoagulant Compound development Exenatide √ Beagle plasma 20-10000 pg/mL EDTA

Exenatide γ Pig plasma 20-10000 pg/mL EDTA

Exenatide √ Human plasma 20-20000 pg/mL EDTA

Apremilast √ Human plasma 2.5-1000 ng/mL EDTA

Bosutinib √ Human plasma 0.1-40 ng/mL EDTA

Carbocysteine √ Human plasma 0.15-15 µg/mL EDTA

Cinacalcet √ Human plasma 0.25-50 ng/mL EDTA Clobazam and N- √ 2-800 ng/mL Human plasma EDTA Desmethylclobazam 0.5-200 ng/mL Clodronic Acid √ Human plasma 5-500 ng/mL EDTA

Clopidogrel √ Human plasma 5-1500 pg/mL EDTA Diosmetin Total and √ 2.8-1000 ng/mL Human plasma EDTA Hesperetin Total 2.9-1000 ng/mL

Page 25 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Entecavir √ Human plasma 0.1-15 ng/mL EDTA

Ergocalciferol √ Human plasma 0.1-30 ng/mL EDTA

Everolimus √ Dried Blood Spots 1-50 ng/mL N/A

Fulvestrant √ Rat plasma 0.5-50 ng/mL EDTA

Genistein √ Rat plasma 1-1000 ng/mL Fluoride/Oxalate √ Human plasma EDTA Ibandronate 0.4-200 ng/mL Human serum N/A Imatinib √ Human plasma 100-7000 ng/mL Heparin

Mefenamic Acid √ Human plasma 5-5000 ng/mL EDTA

Methotrexate √ Human plasma 0.3-150 µg/mL EDTA

Nifedipine √ Human plasma 0.5-200 ng/mL EDTA √ Human plasma Nilotinib 1-500 ng/mL EDTA White cells Nefopam, N- 1-500 ng/mL Desmethylnefopam √ Human plasma 1-500 ng/mL EDTA and N-Oxide Nefopam 1-500 ng/mL Paclitaxel √ Human plasma 30-12000 ng/mL EDTA

Page 26 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Sildenafil and N- √ 1-500 ng/mL Human plasma EDTA Desmethyl Sildenafil 0.5-250 ng/mL Peptide SOR C13 √ Dog plasma 10-2000 ng/mL Heparin

Sunitinib √ Human plasma 0.3-150 ng/mL EDTA

Tartaric Acid √ Human plasma 5-100 ng/mL EDTA

Temozolomide √ Human plasma 0.1-15 µg/mL EDTA

Tenofovir √ Human plasma 5-600 ng/mL EDTA

Teriflunomide √ Human plasma 25-4000 ng/mL EDTA

Trospium Chloride √ Human plasma 0.1-10 ng/mL EDTA 10-4000 ng/mL Ursodiol, Tauroursodiol √ Human plasma 2-800 ng/mL EDTA and Glycoursodiol 20-8000 ng/mL √ Human Whole Vinorelbine 1-300 ng/mL Fluoride/Oxalate Blood

Page 27 of 28

Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays

For further information on our validated assays, please contact your Business development representative or via [email protected]

Latest update: 9-July-2020 Update by: Laurentiu Ciochina Directeur - R&D Director - R&D Tel: +1 450 663 6724 ext: 6419 Cellular +1 514 618 6142 [email protected] www.biotrial.com

BIOTRIAL BIOANALYTICAL SERVICES SOLUTION

A fit-for-purpose flexible analyte profiling, screening, identification, quantification, toxicokinetics, drug and screening cascade to support your medicinal chemistry and bioscience innovative compound efforts. Access to a complete array of standard in vivo and services at worldwide competitive prices. Experienced R&D Scientists can guide you through the discovery process from planning to data delivery for all your innovator drug needs. Method development and validation for small and large molecule assays for the analysis of drugs, metabolites, and biomarkers in a broad range of biological fluids.

Page 28 of 28