Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays For further information on our validated assays, please contact your Business development representative or via [email protected] Compound (Metabolite) Species/Matrix Range Anticoagulant 15-Hydroxy Lubiprostone Human plasma 1-150 pgmL EDTA 15-Hydroxy Lubiprostone 35-7000 pg/mL Rat plasma EDTA Lubiprostone 35-7000 pg/mL 17-Desacetyl-Norgestimate Human plasma 50-5000 pg/mL EDTA 25-Hydroxy-vitamin D2 (Calciferol) Human plasma 5-250 ng/mL EDTA (DEQAS certified) 25-Hydroxy-vitamin D3 (Calcifediol) Human plasma 5-250 ng/mL EDTA (DEQAS certified) 3-Ketodesogestrel Human plasma 0.05-10 ng/mL EDTA Acetaminophen Human plasma 200-10000 ng/mL EDTA Acetazolamine Human plasma 50-20000 ng/mL EDTA Acetylsalicylic Acid Human plasma 5-2000 ng/mL Fluoride/Oxalate 20-8000 ng/mL Acetylsalicylic Acid, Salicylic acid and Human plasma 80-32000 ng/mL Fluoride/Oxalate Butalbital 10-4000 ng/mL Afatinib Human plasma 0.1-50 ng/mL EDTA Albuterol Human plasma 0.25-50 ng/mL EDTA Page 1 of 28 Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays For further information on our validated assays, please contact your Business development representative or via [email protected] Compound (Metabolite) Species/Matrix Range Anticoagulant Alendronate Human urine 1-200 ng/mL N/A Alfuzosin Human plasma 0.15-30 ng/mL EDTA Aliskiren Human plasma 1-500 ng/mL EDTA Alprazolam Human plasma 0.25-25 ng/mL EDTA Amlodipine Human plasma 0.1-15 ng/mL EDTA Anagrelide Human plasma 0.05-11 ng/mL EDTA 0.5-150 ng/mL D,L-Amphetamine Human plasma EDTA 0.25-30 ng/mL Anastrazole Human plasma 0.1-20 ng/mL EDTA Aripiprazole Human plasma 0.5-100 ng/mL EDTA Asenapine Human plasma 0.05-20 ng/mL EDTA Asoxime Human plasma 0.1-30 µg/mL EDTA Asoxime Human urine 1-300 µg/mL N/A Page 2 of 28 Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays For further information on our validated assays, please contact your Business development representative or via [email protected] Compound (Metabolite) Species/Matrix Range Anticoagulant Atomoxetine Human plasma 2-2000 ng/mL EDTA 0.15-150 ng/mL Atorvastatin, O-Hydroxy-Atorvastatin and Human plasma 0.15-150 ng/mL EDTA P-Hydroxy-Atorvastatin) 0.1-15 ng/mL 0.02-20 ng/mL Atorvastatin, O-Hydroxy-Atorvastatin and Human plasma 0.02-20 ng/mL EDTA P-Hydroxy-Atorvastatin 0.01-1.5 ng/mL Atorvastatin Human plasma 0.25-200 ng/mL EDTA Atovaquone Human plasma 50-25000 ng/mL EDTA 5-1000 ng/mL Benazepril and Benazeprilat Human plasma EDTA 7.5-1500 ng/mL 0.5-200 ng/mL Bexarotene Human plasma EDTA 1-500 ng/mL Betamethasone Human plasma 0.5-250 ng/mL Heparin Bezafibrate Human plasma 15-5000 ng/mL EDTA Bicalutamide Human plasma 10-1000 ng/mL EDTA Bicalutamide Chiral S and R form 4-300 ng/mL Human plasma EDTA 16-1200 ng/mL Page 3 of 28 Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays For further information on our validated assays, please contact your Business development representative or via [email protected] Compound (Metabolite) Species/Matrix Range Anticoagulant Bisoprolol Human plasma 1-100 ng/mL EDTA 0.04-8 ng/mL Buprenorphine and Norbuprenorphine Human plasma EDTA 0.02-4 ng/mL 2.5-500 ng/mL Bupropion, Hydroxybupropion, Erythro- 12.5-2500 ng/mL Hydroxybupropion and Threo- Human plasma EDTA 4-800 ng/mL Hydroxybupropion 8-1600 ng/mL 1-200 ng/mL Buproprion and hydroxybupropion Human plasma EDTA 4-800 ng/mL Calcifediol Human plasma 5-250 ng/mL EDTA Calcitriol Human plasma 10-200 pg/mL EDTA Carbamazepine and Carbamazepine 10,11- 25-5000 ng/mL Human plasma EDTA epoxide 1.25-250 ng/mL 1-250 ng/mL Carbidopa and Levodopa Human plasma 8-2000 ng/mL EDTA 2-400 ng/mL Carbidopa and Levodopa Human plasma Fluoride/Oxalate 10-2000 ng/mL 0.5-200 ng/mL Carbidopa, Levodopa and Etilevodopa Human plasma 6.25-2500 ng/mL Fluoride/Oxalate 0.05-20 ng/mL Page 4 of 28 Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays For further information on our validated assays, please contact your Business development representative or via [email protected] Compound (Metabolite) Species/Matrix Range Anticoagulant 10-2500 ng/mL Carisoprodol and Meprobamate Human plasma EDTA 10-2500 ng/mL 5-2500 ng/mL Carisoprodol and Meprobamate Dog plasma EDTA 5-2500 ng/mL 0.5-100 ng/mL Carvedilol and 4-Hydroxyphenyl Carvedilol Human plasma EDTA 0.1-10 ng/mL 0.25-50 ng/mL Carvedilol and 4-Hydroxyphenyl Carvedilol Human plasma EDTA 0.05-10 ng/mL Cellulose acetate butyrate Mini Pig plasma 20-200 µg/mL EDTA Cellulose acetate butyrate Rat plasma 20-200 µg/mL EDTA Chlorambucil and Phenylacetic Acid 5-1600 ng/mL Human serum N/A Mustard 5-1600 ng/mL 0.1-15 ng/mL Cholecalciferol (Vitamin D3), Calcitriol and Human serum 0.01-1.5 ng/mL N/A Calcifediol (25-hydroxy-vitamin D3) 2-300 ng/mL Cholecalciferol (Vitamin D3) Human plasma 0.1-30 ng/mL EDTA 50-8000 ng/mL Ciprofloxacin and Gatifloxacin Human plasma EDTA 50-8000 ng/mL Ciprofloxacin Human plasma 30-6000 ng/mL EDTA Page 5 of 28 Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays For further information on our validated assays, please contact your Business development representative or via [email protected] Compound (Metabolite) Species/Matrix Range Anticoagulant Clarithromycin Human plasma 15-6000 ng/mL EDTA Clarithromycin Human plasma 10-2000 ng/mL EDTA Clonidine Human plasma 1-200 pg/mL EDTA Clonidine Human plasma 10-2000 pg/mL EDTA Clonidine Rabbit plasma 10-2000 pg/mL EDTA 0.5-125 ng/mL Codeine and Caffeine Human plasma EDTA 10-2500 ng/mL Cyclobenzaprine Human plasma 0.05-20 ng/mL EDTA Cyclobenzaprine Human plasma 0.1-40 ng/mL EDTA Cyproterone Acetate Human plasma 0.9-180 ng/mL EDTA Cyproterone Acetate Human plasma 0.25-50 ng/mL EDTA Danazol Human plasma 0.8-200 ng/mL EDTA Darifenacin Human plasma 0.05-10 ng/mL EDTA Page 6 of 28 Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays For further information on our validated assays, please contact your Business development representative or via [email protected] Compound (Metabolite) Species/Matrix Range Anticoagulant Dasatinib Human plasma 0.5-200 ng/mL EDTA 25-6250 pg/mL Desloratadine and 3-Hydroxy-Desloratadine Human plasma EDTA 12-3000 pg/mL Dexmethylphenidate Human plasma 0.15-45 ng/mL EDTA Dexmethylphenidate Human plasma 0.1-30 ng/mL EDTA Diclofenac Human plasma 8-4000 ng/mL EDTA Didanosine Human plasma 10-1500 ng/mL EDTA Dienogest Human plasma 0.5-150 ng/mL EDTA 1.5-500 ng/mL Diltiazem, N-Desmethyl Diltiazem and Human plasma 0.3-100 ng/mL EDTA Desacetyl Diltiazem 0.15-50 ng/mL Dipyridamole Human plasma 10-4000 ng/mL Fluoride/Oxalate Donepezil Human plasma 0.1-20 ng/mL EDTA 0.5-50 ng/mL Donepezil and Memantine Human plasma EDTA 0.5-50 ng/mL Doxazosin Human plasma 0.5-125 ng/mL EDTA Page 7 of 28 Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays For further information on our validated assays, please contact your Business development representative or via [email protected] Compound (Metabolite) Species/Matrix Range Anticoagulant Doxercalciferol and Ercalcitriol Human serum 2-80 pg/mL N/A 2-80 pg/mL Doxercalcifero and Ercalcitriol Human plasma EDTA 2-80 pg/mL Doxycycline Human plasma 5-1000 ng/mL EDTA 0.5-150 ng/mL Doxylamine and Pyridoxine Human plasma EDTA 0.25-100 ng/mL Drospirenone Human plasma 0.5-150 ng/mL EDTA Drospirenone Human plasma 0.075-30 ng/mL EDTA Elesclomol and Metabolites Human plasma 1-1000 ng/mL Heparin 0.5-125 ng/mL Enalapril and Enalaprilat Human plasma EDTA 0.25-62.5 ng/mL Entacapone Human Plasma 25-2500 ng/mL EDTA Enzalutamide Human Plasma 20-8000 ng/mL EDTA 0.5-250 ng/mL EPA ethyl ester and DHA ethyl ester Human plasma EDTA 0.5-250 ng/mL 1.6 -120 µg/mL EPA Total and DHA Total Human plasma EDTA 8-200 µg/mL Page 8 of 28 Biotrial Bioanalytical Services Inc. Global List of Validated Bioanalytical Assays For further information on our validated assays, please contact your Business development representative or via [email protected] Compound (Metabolite) Species/Matrix Range Anticoagulant EPA ethyl ester Human plasma 1-1000 ng/mL EDTA Esomeprazole Human plasma 4-2000 ng/mL EDTA Ethinyl estradiol Human plasma 2-500 pg/mL EDTA Estradiol Free Human plasma 1-150 pg/mL EDTA Estradiol Total Human serum 2-400 pg/mL N/A 4-300 pg/mL Estrone Free and Equilin Free Human plasma EDTA 2-150 pg/mL 10-1000 pg/mL Estrone Free and Estradiol Free Human plasma EDTA 2-200 pg/mL 5-1000 pg/mL Estrone Free, Equilin Free and Delta 8,9- Human plasma 2-400 pg/mL EDTA Dehydroestrone 2-400 pg/mL 4-300 pg/mL Estrone Free, Estradiol Free, Equilin Free 2-150 pg/mL Human plasma EDTA and Dihydroequilin Free 2-150 pg/mL 2-150 pg/mL Human plasma 50-10000 pg/mL EDTA Estrone Total and Equilin Total 50-10000 pg/mL Page 9 of 28 Biotrial Bioanalytical Services Inc.