LASP1 Polyclonal Antibody

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LASP1 polyclonal antibody Catalog # : PAB6082 規格 : [ 100 ug ] List All Specification Application Image Product Goat polyclonal antibody raised against synthetic peptide of LASP1. Western Blot (Tissue lysate) Description: Immunogen: A synthetic peptide corresponding to human LASP1. Sequence: NPNCARCGKIVYP enlarge Host: Goat ELISA Theoretical MW 29.7 (kDa): Reactivity: Human Form: Liquid Purification: Antigen affinity purification Concentration: 0.5 mg/mL Quality Control Antibody Reactive Against Synthetic Peptide. Testing: Recommend ELISA (1:128000) Usage: Western Blot (0.1-0.3 ug/mL) The optimal working dilution should be determined by the end user. Storage Buffer: In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide) Storage Store at -20°C. Instruction: Aliquot to avoid repeated freezing and thawing. Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. Datasheet: Download Publication Reference 1. Organelle proteomics of rat synaptic proteins: correlation-profiling by isotope-coded affinity tagging in conjunction with liquid chromatography-tandem mass spectrometry to reveal post-synaptic density specific proteins. Li K, Hornshaw MP, van Minnen J, Smalla KH, Gundelfinger ED, Smit AB.J Proteome Res. 2005 May-Jun;4(3):725-33. Applications Western Blot (Tissue lysate) Page 1 of 2 2017/2/16 LASP1 polyclonal antibody (Cat # PAB6082) staining (0.1 ug/mL) of human brain (cerebellum) extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ELISA Gene Information Entrez GeneID: 3927 Protein NP_006139.1 Accession#: Gene Name: LASP1 Gene Alias: Lasp-1,MLN50 Gene LIM and SH3 protein 1 Description: Omim ID: 602920 Gene Ontology: Hyperlink Gene Summary: This gene encodes a member of a LIM protein subfamily characterized by a LIM motif and a domain of Src homology region 3. The encoded protein functions as an actin-binding protein and possibly in cytoskeletal organization. [provided by RefSeq Other - Designations: Related Disease Autistic Disorder Genetic Predisposition to Disease Tobacco Use Disorder 服務條款 | 隱私權政策 | 著作及商標 | 網站地圖 ©2017 亞諾法生技股份有限公司 Abnova Corporation. 版權所有. Page 2 of 2 2017/2/16.

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(LASP1) in the Metastatic Dissemination of Medulloblastoma
Published OnlineFirst October 5, 2010; DOI: 10.1158/0008-5472.CAN-10-0592 Published OnlineFirst on October 5, 2010 as 10.1158/0008-5472.CAN-10-0592 Therapeutics, Targets, and Chemical Biology Cancer Research Role of LIM and SH3 Protein 1 (LASP1) in the Metastatic Dissemination of Medulloblastoma Christopher Traenka1, Marc Remke2,5, Andrey Korshunov4,6, Sebastian Bender2,5, Thomas Hielscher3, Paul A. Northcott7, Hendrik Witt2,5, Marina Ryzhova8, Jörg Felsberg9, Axel Benner3, Stephanie Riester2, Wolfram Scheurlen10, Thomas G.P. Grunewald11, Andreas von Deimling6, Andreas E. Kulozik5, Guido Reifenberger9, Michael D. Taylor7, Peter Lichter2, Elke Butt1, and Stefan M. Pfister2,5 Abstract Medulloblastoma is the most common malignant pediatric brain tumor and is one of the leading causes of cancer-related mortality in children. Treatment failure mainly occurs in children harboring metastatic tumors, which typically carry an isochromosome 17 or gain of 17q, a common hallmark of intermediate and high-risk medulloblastoma. Through mRNA expression profiling, we identified LIM and SH3 protein 1 (LASP1) as one of the most upregulated genes on chromosome 17q in tumors with 17q gain. In an independent validation cohort of 101 medulloblastoma samples, the abundance of LASP1 mRNA was significantly associated with 17q gain, metastatic dissemination, and unfavorable outcome. LASP1 protein expression was analyzed by immuno- histochemistry in a large cohort of patients (n = 207), and high protein expression levels were found to be strongly correlated with 17q gain, metastatic dissemination, and inferior overall and progression-free survival. In vitro experiments in medulloblastoma cell lines showed a strong reduction of cell migration, increased adhesion, and decreased proliferation upon LASP1 knockdown by small interfering RNA–mediated silencing, further indicating a functional role for LASP1 in the progression and metastatic dissemination of medullo- blastoma.
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