Anarchic Centromeres: Deciphering Order from Apparent Chaos
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Wellcome Four Year Phd Programme in Integrative Cell Mechanisms
2021 Wellcome Four Year PhD Programme in Integrative Cell Mechanisms Training the next generation of Molecular Cell Biologists Background and Aims of Programme The Wellcome Four Year PhD Programme in Integrative Cell Mechanisms (iCM) is closely associated with the Wellcome Centre for Cell Biology and trains the next generation of cell and molecular biologists in the application of quantitative methods to understand the inner workings of distinct cell types in different settings. A detailed understanding of normal cellular function is required to investigate the molecular cause of disease and design future treatments. However, data generated by biological research requires increasingly complex analysis with technological advances in sequencing, mass spectrometry/proteomics, super-resolution microscopy, Wellcome Centre for Cell Biology 2021 synthetic and structural biology generating increasingly large, complex datasets. In addition, innovations in computer sciences and informatics are transforming data acquisition and analysis and breakthroughs in physics, chemistry and engineering allow the development of devices, molecules and instruments that drive the biological data revolution. Exploiting technological advances to transform our understanding of cellular mechanisms will require scientists who have been trained across the distinct disciplines of natural sciences, engineering, informatics and mathematics. To address this training need, iCM PhD projects are cross-disciplinary involving two primary supervisors with complementary expertise. Supervisor partnerships pair quantitative scientists with cell biologists ensuring that students develop pioneering cross-disciplinary collaborative projects to uncover cellular mechanisms relevant to health and disease. We aim to recruit students with a variety of backgrounds across the biological and physical sciences, including Biochemistry, Biomedical Science, Cell Biology, Chemistry, Computational Data Sciences, Engineering, Genetics, Mathematics, Molecular Biology and Physics. -
EMBO Conference on Fission Yeast: Pombe 2013 7Th International Fission Yeast Meeting London, United Kingdom, 24 - 29 June 2013
Abstracts of papers presented at the EMBO Conference on Fission Yeast: pombe 2013 7th International Fission Yeast Meeting London, United Kingdom, 24 - 29 June 2013 Meeting Organizers: Jürg Bähler UCL, UK Jacqueline Hayles CRUK-LRI, UK Scientific Programme: Robin Allshire UK Rob Martienssen USA Paco Antequera Spain Hisao Masai Japan Francois Bachand Canada Jonathan Millar UK Jürg Bähler UK Sergio Moreno Spain Pernilla Bjerling Sweden Jo Murray UK Fred Chang USA Toru Nakamura USA Gordon Chua Canada Chris Norbury UK Peter Espenshade USA Kunihiro Ohta Japan Kathy Gould USA Snezhka Oliferenko Singapore Juraj Gregan Austria Janni Petersen UK Edgar Hartsuiker UK Paul Russell USA Jacqueline Hayles UK Geneviève Thon Denmark Elena Hidalgo Spain Iva Tolic-Nørrelykke Germany Charlie Hoffman USA Elizabeth Veal UK Zoi Lygerou Greece Yoshi Watanabe Japan Henry Levin USA Jenny Wu France These abstracts may not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the authors. Printed by SLS Print, London, UK Page 1 Poster Prize Judges: Coordinated by Sara Mole & Mike Bond UCL, UK Poster Prizes sponsored by UCL, London’s Global University Rosa Aligué Spain Hiroshi Murakami Japan José Ayté Spain Eishi Noguchi USA Hugh Cam USA Martin Převorovský Czech Republic Rafael Daga Spain Luis Rokeach Canada Jacob Dalgaard UK Ken Sawin UK Da-Qiao Ding Japan Melanie Styers USA Tim Humphrey UK Irene Tang USA Norbert Käufer Germany Masaru Ueno Japan Makoto Kawamukai Japan -
Laboratory Harbor Spring Cold Annual Report 1990
YEAR CENTENNIAL THE LABORATORY HARBOR SPRING COLD ANNUAL REPORT 1990 COLD SPRING HARBOR LABORATORY THE CENTENNIAL YEAR ANNUAL REPORT 1990 Cold Spring Harbor Laboratory Box 100 1 Bungtown Road Cold Spring Harbor, New York 11724 Book design Emily Harste Editors Dorothy Brown, Lee Martin Photography Margot Bennett, Herb Parsons, Edward Campodonico Typography Marie Sullivan Front cover: Jones Laboratory, during the Centennial fireworks dis- play. This building, completed in 1893, was the first structure built expressly for science at Cold Spring Harbor. (Photograph by Ross Meurer and Margot Bennett.) Back Cover: (Top) Centennial reenactment of the first biology class at Cold Spring Harbor on board the launch "Rotifer." (Inset) The original class of 1890. Reenactments for the Centennial were directed by Rob Gensel. (Photograph by Randy Wilfong.) (Bottom) Centennial reenactment of the Laboratory's first biology class on the Jones Laboratory porch. (Inset) The original 1890 class on the porch of the old Fish Hatchery building before the completion of Jones Laboratory. (Photograph by Margot Bennett.) Contents Officers of the Corporation/Board of Trustees v Governance and Major Affiliations vi Committees vii Edward Pulling (1898-1991)viii DIRECTOR'S REPORT DEPARTMENTAL REPORTS 21 Administration 23 Buildings and Grounds 25 Development 28 Library Services 30 Public Affairs 32 RESEARCH 35 Tumor Viruses 37 Molecular Genetics of Eukaryotic Cells 93 Genetics 155 Structure and Computation 197 Neuroscience 213 CSH Laboratory Junior Fellows217 COLD SPRING -
Epigenetic Gene Silencing by Heterochromatin Primes Fungal Resistance
bioRxiv preprint doi: https://doi.org/10.1101/808055; this version posted October 17, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Epigenetic gene silencing by heterochromatin primes fungal resistance Sito Torres-Garcia, Pauline N. C. B. Audergon†, Manu Shukla, Sharon A. White, Alison L. Pidoux, Robin C. Allshire* Wellcome Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Mayfield Road, Edinburgh EH9 3BF, UK. † Present address: Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona 08003, Spain * Corresponding author: E-mail: [email protected] bioRxiv preprint doi: https://doi.org/10.1101/808055; this version posted October 17, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Summary: 2 Genes embedded in H3 lysine 9 methylation (H3K9me)–dependent 3 heterochromatin are transcriptionally silenced1-3. In fission yeast, 4 Schizosaccharomyces pombe, H3K9me heterochromatin silencing can be 5 transmitted through cell division provided the counteracting demethylase Epe1 6 is absent4,5. It is possible that under certain conditions wild-type cells might 7 utilize heterochromatin heritability to form epimutations, phenotypes mediated 8 by unstable silencing rather than changes in DNA6,7. Here we show that resistant 9 heterochromatin-mediated epimutants are formed in response to threshold 10 levels of the external insult caffeine. ChIP-seq analyses of unstable resistant 11 isolates revealed new distinct heterochromatin domains, which in some cases 12 reduce the expression of underlying genes that are known to confer resistance 13 when deleted. -
Directory 2016/17 the Royal Society of Edinburgh
cover_cover2013 19/04/2016 16:52 Page 1 The Royal Society of Edinburgh T h e R o Directory 2016/17 y a l S o c i e t y o f E d i n b u r g h D i r e c t o r y 2 0 1 6 / 1 7 Printed in Great Britain by Henry Ling Limited, Dorchester, DT1 1HD ISSN 1476-4334 THE ROYAL SOCIETY OF EDINBURGH DIRECTORY 2016/2017 PUBLISHED BY THE RSE SCOTLAND FOUNDATION ISSN 1476-4334 The Royal Society of Edinburgh 22-26 George Street Edinburgh EH2 2PQ Telephone : 0131 240 5000 Fax : 0131 240 5024 email: [email protected] web: www.royalsoced.org.uk Scottish Charity No. SC 000470 Printed in Great Britain by Henry Ling Limited CONTENTS THE ORIGINS AND DEVELOPMENT OF THE ROYAL SOCIETY OF EDINBURGH .....................................................3 COUNCIL OF THE SOCIETY ..............................................................5 EXECUTIVE COMMITTEE ..................................................................6 THE RSE SCOTLAND FOUNDATION ..................................................7 THE RSE SCOTLAND SCIO ................................................................8 RSE STAFF ........................................................................................9 LAWS OF THE SOCIETY (revised October 2014) ..............................13 STANDING COMMITTEES OF COUNCIL ..........................................27 SECTIONAL COMMITTEES AND THE ELECTORAL PROCESS ............37 DEATHS REPORTED 26 March 2014 - 06 April 2016 .....................................................43 FELLOWS ELECTED March 2015 ...................................................................................45 -
Centromere Repositioning Causes Inversion of Meiosis and Generates a Reproductive Barrier
Centromere repositioning causes inversion of meiosis and generates a reproductive barrier Min Lua and Xiangwei Hea,1 aMinistry of Education Key Laboratory of Biosystems Homeostasis & Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute, Zhejiang University, 310058 Hangzhou, Zhejiang, China Edited by J. Richard McIntosh, University of Colorado, Boulder, CO, and approved September 20, 2019 (received for review July 10, 2019) The chromosomal position of each centromere is determined postulated that a neocentromere may seed the formation of an epigenetically and is highly stable, whereas incremental cases ENC at a site devoid of satellite DNA, which is then matured have supported the occurrence of centromere repositioning on an through acquisition of repetitive DNA. ENCs and neocentromeres evolutionary time scale (evolutionary new centromeres, ENCs), are considered as two sides of the same coin, manifestations of the which is thought to be important in speciation. The mechanisms same biological phenomenon at drastically different time scales underlying the high stability of centromeres and its functional and population sizes (7). Hence, understanding centromere repo- significance largely remain an enigma. Here, in the fission yeast sitioning may provide mechanistic insights into ENC emergence Schizosaccharomyces pombe, we identify a feedback mechanism: and progression. The kinetochore, whose assembly is guided by the centromere, in CENP-A–containing chromatin directly recruits specific com- turn, enforces centromere stability. Upon going through meiosis, ponents of the kinetochore, called the constitutive centromere- specific inner kinetochore mutations induce centromere reposi- associated network. The kinetochore is a proteinaceous ma- tioning—inactivation of the original centromere and formation chinery comprised of inner and outer parts, each compassing of a new centromere elsewhere—in 1 of the 3 chromosomes at several subcomplexes. -
Symposium 2019 Booklet 4Website II.Pdf
CONTENTS Foreword……………………………………………………………………………………… 3 Symposium programme ………………………………………………………………. 4 Meet our speakers ……………………………………………………………………….. 5 Student abstracts …………………………………………………………………………. 9 Theme 1: Genetic Processes and Proteins ………………………….. 10 Theme 2: Environmental Biology and Ecology …………………… 17 Theme 3: Health and Nutrition …………………………………………... 23 Theme 4: Fundamental meets Synthetic Biology ………………… 29 Theme 5: Body Brain and Behaviour ………………………………….. 34 Poster design: © Liat Adler 2 Welcome to the EASTBIO Annual Symposium 2019 A very warm welcome to attendees at the 2019 Annual Symposium of the BBSRC-funded EASTBIO Doctoral Training Partnership. The Annual Symposium represents one of the highlights in the EASTBIO calendar. The theme of this year’s conference is ‘Bioscience Research: To Biology and Beyond!’ The two-day Symposium brings together guest speakers and four cohorts of our PhD students to discuss the broad range of interdisciplinary research conducted across the partnership spanning from bioscience for health to biotechnology and food security. We hope you will enjoy the proceedings! Dr Edgar Huitema School of Life Sciences, University of Dundee On behalf of the EASTBIO Management Group & the Symposium Organising Committee 3 EASTBIO ANNUAL RESEARCH SYMPOSIUM: TO BIOLOGY AND BEYOND! University of Dundee, Dalhousie Building - 13-14 June 2019 Day 1 Schedule – 13 June 2019 10:30 Registration & coffee/tea The Street, School of Life Sciences - note different venue 11:00-11:10 Welcome & Introduction Dalhousie, Lecture -
(12) United States Patent (10) Patent No.: US 6,573,099 B2 Graham (45) Date of Patent: *Jun
USOO6573099B2 (12) United States Patent (10) Patent No.: US 6,573,099 B2 Graham (45) Date of Patent: *Jun. 3, 2003 (54) GENETIC CONSTRUCTS FOR DELAYING OTHER PUBLICATIONS OR REPRESSING THE EXPRESSION OF A TARGET GENE Anderson WF, Nature 392:25-30, 1998.* Verma et al Nature 389:239-242, 1997.* (75) Inventor: Michael Wayne Graham, St. Lucia Touchette, Nat. Med. 2(1) 7–8, 1996.* Wang et al PNAS 94:11563-11566, 1997.* (AU) Wall RJ Theriogenology 45:57-68, 1996.* (73) Assignee: Benitec Australia, Ltd., Queensland Kappel et al. Current Opinion in Biotechnology 3:548-553 (AU) 1992.* Viville, in Transgenic Animals, Houdebine (eds), Harwood (*) Notice: This patent issued on a continued pros academic publishers, France. pp307-321, 1997.* ecution application filed under 37 CFR Napoli, Carolyn et al., “Introduction of a Chimeric Chalcone 1.53(d), and is subject to the twenty year Synthase Gene into Petunia Results in Reversible Co-Sup patent term provisions of 35 U.S.C. pression of Homologous Genes in trans”, The Plant Cell, 2: 154(a)(2). 279-289 (1990). Lindbo, John et al., “Induction of a Highly Specific Antiviral Subject to any disclaimer, the term of this State in Transgenic Plants: Implications for Regulation of patent is extended or adjusted under 35 Gene Expression and Virus Resistance”, The Plant Cell, 5: U.S.C. 154(b) by 0 days. 1749–1759 (1993). Park, Y. et al., “Gene Silencing mediated by promoter (21) Appl. No.: 09/100,812 homology occurs at the level of transcription and results in meiotically heritable alterations in methylation and gene (22) Filed: Jun. -
Conference Program
Chromatin and Epigenetics: Inheritance and Design April 1-3, 2019 Munich, Germany Conference Program Conference Program Monday, April 1 11:30 – 13:00 Panel discussion: Ethics in research publishing (Room 041 Building 36.20) 13:00 – 14:00 Coffee and Registration Session 1: Chairs: Maria Colomé-Tatché and Robert Schneider Reading and writing modifications 14:00 – 14:15 Welcome 14:15 – 14:45 Kristian Helin (Memorial Sloan Kettering Cancer Center, US) Role of the Polycomb repressive complex 2 (PRC2) in transcriptional regulations and cancer 14:45 – 15:15 Karmella Haynes (Emory University, US) Synthetic reader-effectors for epigenetic reprogramming of genes in cancer 15:15 – 15:35 Philipp Voigt (Wellcome Trust Centre for Cell Biology, UK) Nucleosomal Asymmetry Shapes Histone Mark Binding at Bivalent Domains 15:35 – 15:55 Marc Timmers (German Cancer Research Center. Germany) Chaperones and Transcription Complex Assembly 15:55 – 16:30 Coffee break 16:30 – 16:50 Sophie Polo (CNRS/Universite Paris Diderot, France) Epigenome maintenance in response to DNA damage 16:50 – 17:10 Raffaela Santoro (University of Zurich, Switzerland) NoRC complex safeguards genome architecture of ground-state pluripotent stem cells 17:10 – 17:30 Valentina Ignatova (Helmholtz Zentrum München, Germany) Characterization of novel RNA methyltransferases 17:30 – 18:00 Tony Kouzarides (The Gurdon Insitute, UK) Modifications of RNA: their function and role in cancer 18:00 – 18:35 Poster Flash Talks Free evening Tuesday, April 2 Session 2: Chairs: Manolis Papamichos-Chronakis -
A Novel Fission Yeast Kinetochore Protein Required for Centromeric
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by PubMed Central JCBArticle Sim4: a novel fission yeast kinetochore protein required for centromeric silencing and chromosome segregation Alison L. Pidoux,1,2 William Richardson,2 and Robin C. Allshire1,2 1Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, UK 2Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, UK ission yeast centromeres are composed of two domains: encodes a known kinetochore protein, the centromere- the central core and the outer repeats. Although both specific histone H3 variant Cnp1CENP-A. sim4ϩ encodes a Fregions are required for full centromere function, the novel essential coiled-coil protein, which is specifically central core has a distinct chromatin structure and is likely associated with the central core region and is required for to underlie the kinetochore itself, as it is associated with the unusual chromatin structure of this region. Sim4 centromere-specific proteins. Genes placed within either coimmunoprecipitates with the central core component region are transcriptionally silenced, reflecting the formation Mis6 and, like Mis6, affects Cnp1CENP-A association with the of a functional kinetochore complex and flanking centro- central domain. Functional Mis6 is required for Sim4 lo- meric heterochromatin. Here, transcriptional silencing was calization at the kinetochore. Our analyses illustrate the exploited to identify components involved in central core fundamental link between silencing, chromatin structure, silencing and kinetochore assembly or structure. The resulting and kinetochore function, and establish defective silencing sim (silencing in the middle of the centromere) mutants as a powerful approach for identifying proteins required to display severe chromosome segregation defects. -
An Open Letter to the Government of Ireland
An Open Letter to the Government of Ireland As scientists in Ireland and Irish scientists abroad, we are committed to making our contribution to Ireland’s recovery by doing the best and most innovative research possible. However, we are deeply concerned about the research policies implemented by the current government. The policy of sustained investment in scientific excellence that helped build a vibrant scientific community in Ireland over the past fifteen years has given way to a short- sighted drive for commercialisable research in a very limited set of prescribed areas. Along with an investment in research that is below the EU average, steadily decreasing core grants to universities, and a constant demand to increase student numbers, these policies are creating a perfect storm for scientific research and education in Ireland and are undermining our abilities to carry out worldclass research, to retain scientific talent in the country and also to educate future scientists and build a real and sustainable knowledge economy. Innovation needs a strong core in basic research A wealth of economic research shows that sustained investment in basic research pays huge dividends economically, not just through the generation of intellectual property and the development of new companies, but also by building human capital and by attracting companies that hope to benefit from a vibrant research community. Countries with long- established and functional research systems that successfully underpin economic development rely on a well-balanced mix between basic and applied research. The Irish Government’s current investment in applied research is welcome and forms an essential part of an overall strategy to generate economic return from scientific research. -
40TH FEBS CONGRESS the Biochemical Basis of Life
40TH FEBS CONGRESS The Biochemical Basis of Life July 4–9, 2015 • Berlin, Germany www.febs2015.org Berlin Partner/FTB-Werbefotografi e Berlin Partner/FTB-Werbefotografi FINAL PROGRAM We would liketothankourstrategicpartners: We Acknowledgements Saturday July 4 Sunday July 5 Monday July 6 Tuesday July 7 Wednesday July 8 Thursday July 9 8:30 AM–11:00 AM 8:30 AM–11:00 AM 8:30 AM–11:00 AM 8:30 AM–11:00 AM 8:30 AM–11:00 AM 9:00 AM Parallel Symposia Parallel Symposia Parallel Symposia Parallel Symposia Parallel Symposia 10:00 AM 11:00 AM Coffee Break Coffee Break Coffee Break Coffee Break Coffee Break 11:30 AM–12:30 PM 11:30 AM–12:30 PM 11:30 AM–12:30 PM 11:30 AM–12:30 PM 11:30 AM–12:00 PM IUBMB Lecture FEBS | EMBO Women in Science Award PABMB Lecture Sir Hans Krebs Lecture Otto Meyerhof Prize Xiaowei Zhuang Caroline Dean Alberto R. Kornblihtt Jürgen Knoblich 12:00 PM 12:00 PM–1:00 PM The EMBO Lecture Sarah A. Teichmann 12:30 PM–2:30 PM Industry Women in Science Lunch Break Symposium Lunch Break Lunch Break Lunch Break Lunch Break 1:00 PM Luncheon 1:00 PM–1:15 PM Closing Remarks & Farewell 1:00 PM–3:00 PM 1:30 PM–3:00 PM 1:30 PM–3:00 PM 1:30 PM–3:00 PM 1:30 PM–3:00 PM FEBS Education 1:30 PM–3:00 PM 1:30 PM–3:00 PM 1:30 PM–3:00 PM Speed Talks Posters on Display Speed Talks Posters on Display Session Posters on Display Speed Talks Posters on Display Poster Discussion Poster Discussion Poster Discussion Poster Discussion 2:00 PM Session 1 Session 1 Session 2 Session 2 3:00 PM 3:00 PM–8:45 PM 3:00 PM–5:00 PM 3:00 PM–5:00 PM 3:00 PM–5:00 PM 3:00 PM–5:00