DOCSLIB.ORG

Prepared Microscope Slides in Systematic Order 49 PREPARED MICROSCOPE SLIDES in SYSTEMATIC ORDER

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Prepared Microscope Slides in Systematic Order 49 PREPARED MICROSCOPE SLIDES in SYSTEMATIC ORDER Prepared Microscope Slides in Systematic Order 49 PREPARED MICROSCOPE SLIDES IN SYSTEMATIC ORDER The list of the available microscopic specimens was also revised and further essentially completed. Their systematic arrangement facilitates the finding of slides necessary to compile series for special use. A detailed list of contents is found on page 76. Helpful for orientation are the • marked slides of important specimens which are characteristic and representative of the taxonomic group or of the subject. Various slides are available only in small number or have a long delivery period, as their material is either rare or causes unusual difficulties in processing. This applies particularly to the slides marked with an asterisk * in the catalogue, for which we cannot guarantee delivery. Abbreviations: t.s. transverse or cross section l.s. longitudinal section w.m. whole mount or entire specimen Pr2114d Phacus, flat heart-shaped cells w.m. Pr2115e Trachelomonas, a free swimming species of PROTOZOA the Euglenophyta Pr212c • Ceratium hirundinella, a fresh water di- Rhizopoda (Sarcodina) noflagellate w.m. Pr2121c Ceratium, slide showing different marine forms Pr112e • Amoeba proteus, showing nucleus, endo- w.m. plasm, ectoplasm, food vacuoles, pseudopodia Pr2123d Peridinium, a fresh water dinoflagellate w.m. Pr112e Pr211c w.m. Pr213d • Noctiluca miliaris, a large marine flagellate Pr113f Amoeba proteus, section through specimens causing the phosphorescence of the sea, w.m. Pr114f • Entamoeba histolytica, causes amebic dys- Pr225h Chilomastix mesnili, flagellate found in human entery, smear from feces intestine, nonpathogenic, smear with trophozo- Pr1141h Entamoeba histolytica, causes amebic dys- ites * entery, smear showing trophozoites (asexual Pr2252h Chilomastix mesnili, smear with cysts forms) * Pr221h Giardia lamblia intestinalis, human parasite, Pr1142h Entamoeba histolytica, causes amebic dys- smear with trophozoites * Pr1142h entery, smear showing cysts * Pr2212h Giardia lamblia intestinalis, smear showing Pr212c Pr115g Entamoeba histolytica, section through dis- cysts * eased colon showing the parasites in situ Pr223f • Trichomonas sp., smear with trophozoites Pr116g • Entamoeba coli, nonpathogenic, smear from Pr2232h Trichomonas vaginalis, smear * feces Pr2233h Trichomonas muris, trophozoites Pr1161h Entamoeba coli, nonpathogenic, smear with Pr230f • Trypanosoma gambiense, a blood flagellate, trophozoites * causing Central African sleeping disease, blood Pr1162h Entamoeba coli, smear showing cysts * smear Pr1165h Entamoeba hartmanni trophozoites. Smear, Pr231f Trypanosoma rhodesiense, causes South Pr1162h Pr213d intestinal amoeba; nonpathogenic to humans African sleeping disease, blood smear with Pr1166h Entamoeba hartmanni cysts. Smear parasites Pr1168h Dientamoeba fragilis trophozoites. Smear Pr232f • Trypanosoma evansi, causes surra in cattle, Pr117f Entamoeba invadens, large specimens from blood smear culture, good for demonstration Pr233f • Trypanosoma brucei, causes nagana, blood Pr1173g Entamoeba gingivalis, smear with trophozoi- smear tes Pr234f Trypanosoma congolense, pathogenic to do- Pr1174h Endolimax nana, small human parasite, smear mestic animals, blood smear Pr115g with trophozoites * Pr235f • Trypanosoma equiperdum, dourine in hors- Pr221h Pr1175h Endolimax nana, smear with cysts * es, blood smear Pr1177h Jodamoeba butschlii, a commensal living in Pr236f • Trypanosoma cruzi (Schizotrypanum), caus- the human intestine, smear with trophozoites * es Chagas disease of man, blood smear show- Pr1178h Jodamoeba butschlii, smear with uninucleate ing trypanosomes cysts * Pr237g • Trypanosoma cruzi, section through infected Pr1181v Pneumocystis carinii. Smear from lung tissue heart muscle shows Leishmania forms in tis- stained to show cyst wall of parasites * sue * Pr114f Pr1182v Pneumocystis carinii. Smear from lung tissue Pr2372h Trypanosoma cruzi. Smear from culture show- Pr223f stained to show trophozoites and sporozoites * ing cultured forms * Pr119d • Arcella, shelled amoeba w.m. Pr2373g Trypanosoma cruzi. Leishmania forms in sec. Pr1195s Actinosphaerium, a fresh water actinopode of mouse brain * w.m. * Pr2374g Trypanosoma cruzi. Leishmania forms in sec. Pr121d • Radiolaria, mixed species showing different of mouse liver * forms Pr2375g Trypanosoma cruzi. Leishmania forms in sec. Pr122d • Foraminifera, mixed species showing different of mouse heart muscle fibres * forms Pr2376g Trypanosoma cruzi. Leishmania forms in sec. Pr119d Pr1251d Foraminifera from Mediterranean sea, mixed of mouse spleen Pr2232h recent Pr241f Trypanosoma lewisi, a large species living in Pr1252d Foraminifera, mixed fossil, chalk rats and mice, blood smear with parasite, heavy Pr124d Foraminifera, mixed forms from the Adriatic infection Sea Pr2413g • Trypanosoma lewisi, blood smear, early stag- Pr123d • Globigerina, marine forms, mixed species es of infection with division stages Pr2414g Trypanosoma lewisi, blood smear, later stag- Flagellata (Mastigophora) es of infection, large forms * Pr121d Pr238f • Leishmania donovani, causes Kala-Azar, Pr230f Pr211c • Euglena viridis, a common green flagellate smear from the infected spleen showing the with eyespot and flagellum, w.m. typical Leishman-Donovan bodies • Pr2112c Euglena gracilis, a smaller species, w.m. Pr239g Leishmania donovani, section through infect- Pr2113f Euglena, a large species specially fixed and ed spleen or liver showing the parasites within stained to show the flagella, w.m. the cells Pr122d Pr236f 50 Prepared Microscope Slides in Systematic Order Pr2392t Leishmania donovani, smear from culture Pr3392f Sarcocystis tenella in heart muscle, sec. showing Leishman and leptomonad forms * Pr3365s Myxosoma, parasite on fish gill, sec. * Pr2395h Leishmania donovani, promastigotes, smear from culture * Ciliata (Infusoria) Pr2396h Leishmania donovani, amastigotes, smear from tissue * Pr411d • Paramecium, macro- and micronuclei stained. Pr2397h Leishmania mexicana, promastigotes, smear The typical slide for general study of this com- from culture * Pr237g mon ciliate Pr339f Pr240f Leishmania enrietti, section through nasal Pr412e Paramecium, food vacuoles and nuclei doubly abscess from Guinea pig. Very heavy infection stained Pr2405g Crithidia fasciculata, smear from intestine of Pr413e Paramecium, pellicle stained after Bresslau Anopheles mosquito showing the typical crithid- Pr414e Paramecium, silver stained to show the silver ia forms * line or neuroformative system Pr2378g Termite flagellates. w.m., showing large veg- Pr415e Paramecium, specially prepared and stained etative forms * to show the trichocysts • Pr251d Silicoflagellates, various species Pr416f • Paramecium, in conjugation, nuclei stained * Pr238f Pr417g • Paramecium, in fission, nuclei stained * Pr412e Pr418e Paramecium, section through many individu- Sporozoa als, triply stained Pr419f Paramecium, stained with Feulgen reaction Pr311f • Plasmodium falciparum, malignant tertian Pr4194e Paramecium multimicronucleatum, w.m. nu- malaria of man, blood smear with typical ring clei stained. this species contains several mi- stages cronuclei Pr3112g Plasmodium falciparum, blood smear with Pr4195e Paramecium aurelia, w.m. nuclei stained. This Pr239g more gametocytes * species containing one macronucleus and two Pr413e Pr312f Plasmodium falciparum, thick diagnostic micronuclei smear * Pr4196e Paramecium bursaria, w.m. and nuclei Pr313h Plasmodium vivax, benign tertian malaria of stained, showing symbiotic zoochlorellae in man, blood smear * endoplasm Pr3132h Plasmodium vivax, thick diagnostic blood Pr422e • Vorticella, a common stalked ciliate w.m. smear * Pr4222e Vorticella, a marine species, coloniate ciliate Pr3145h Plasmodium malariae, causing quartan ma- Pr421d • Stylonychia, a common ciliate w.m. laria, blood smear * Pr430e • Colpidium, a common holotrich ciliate Pr311f Pr415e Pr315f • Plasmodium berghei, blood smear from ex- Pr427f Spirostomum ambiguum, a ciliate with very perimentally infected mouse. Very heavy infec- large nucleus tion shows abundant parasites in different stag- Pr428g Stentor, a trumpet-shaped large ciliate * es of development Pr429e • Euplotes, a common marine ciliate Pr320h Plasmodium sp., section through infected Pr4306f Bursaria truncatella, a large fresh water cili- mosquito stomach with oocysts containing ate * sporozoites * Pr4309e Blepharisma, a large ciliate with pigment gran- Pr321i Plasmodium sp., section through the salivary ules * Pr315f gland of infected mosquito with sporozoites * Pr4305e Didinium nasutum, a small ciliate parasite on Pr416f Pr322h Plasmodium sp., exoerythrocytic stages in Paramecium * sec. of brain * Pr423f Dendrocometes paradoxus, suctorial infuso- Pr323h Plasmodium sp., exoerythrocytic stages in ria on the gills of Gammarus * sec. of liver * Pr424f Trichodina domerguei, parasite living on fish Pr3235g Malaria melanemia in human spleen, sec. gills * showing pigment granules in endothelium and Pr4307e • Ephelota, a stalked marine suctorian * Kupffer’s cells Pr4311e Suctoria, marine species Pr326f Plasmodium praecox, avian malaria, blood Pr425f Opalina ranarum, smear from frog intestine Pr320h Pr417g smear Pr426e • Opalina ranarum, in section through frog in- Pr327f • Plasmodium gallinaceum (Proteosoma), fowl testine malaria, blood smear from chicken * Pr4265t Balantidium coli, human parasite, smear with Pr328f Plasmodium cathemerium, avian malaria, trophozoites * blood smear * Pr4266t Balantidium coli, smear with cysts * Pr3285s Plasmodium circumflexum, smear
Load more

Recommended publications
  • The Functional Parasitic Worm Secretome: Mapping the Place of Onchocerca Volvulus Excretory Secretory Products
    pathogens Review The Functional Parasitic Worm Secretome: Mapping the Place of Onchocerca volvulus Excretory Secretory Products Luc Vanhamme 1,*, Jacob Souopgui 1 , Stephen Ghogomu 2 and Ferdinand Ngale Njume 1,2 1 Department of Molecular Biology, Institute of Biology and Molecular Medicine, IBMM, Université Libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, 6041 Gosselies, Belgium; [email protected] (J.S.); [email protected] (F.N.N.) 2 Molecular and Cell Biology Laboratory, Biotechnology Unit, University of Buea, Buea P.O Box 63, Cameroon; [email protected] * Correspondence: [email protected] Received: 28 October 2020; Accepted: 18 November 2020; Published: 23 November 2020 Abstract: Nematodes constitute a very successful phylum, especially in terms of parasitism. Inside their mammalian hosts, parasitic nematodes mainly dwell in the digestive tract (geohelminths) or in the vascular system (filariae). One of their main characteristics is their long sojourn inside the body where they are accessible to the immune system. Several strategies are used by parasites in order to counteract the immune attacks. One of them is the expression of molecules interfering with the function of the immune system. Excretory-secretory products (ESPs) pertain to this category. This is, however, not their only biological function, as they seem also involved in other mechanisms such as pathogenicity or parasitic cycle (molting, for example). Wewill mainly focus on filariae ESPs with an emphasis on data available regarding Onchocerca volvulus, but we will also refer to a few relevant/illustrative examples related to other worm categories when necessary (geohelminth nematodes, trematodes or cestodes).
    [Show full text]
  • Exposure to Parasitic Protists and Helminths Changes the Intestinal Community Structure Of
    bioRxiv preprint doi: https://doi.org/10.1101/717165; this version posted July 28, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Title: Exposure to parasitic protists and helminths changes the intestinal community structure of 2 bacterial microbiota but not of eukaryotes in a cohort of mother-child binomial from a semi-rural 3 setting in Mexico 4 Running title: Parasites affect intestinal microbiome 5 Oswaldo Partida-Rodriguez1,2, Miriam Nieves-Ramirez1,2, Isabelle Laforest-Lapointe3,4, Eric Brown2, 6 Laura Parfrey5,6, Lisa Reynolds2, Alicia Valadez-Salazar1, Lisa Thorson2, Patricia Morán1, Enrique 7 Gonzalez1, Edgar Rascon1, Ulises Magaña1, Eric Hernandez1, Liliana Rojas-V1, Javier Torres7, Marie 8 Claire Arrieta2,3,4*, Cecilia Ximenez1*#, Brett Finlay2,8,9* 9 * Senior authors, contributed equally. 10 1Laboratorio de Inmunología del Departamento de Medicina Experimental, UNAM, Mexico City, Mexico 11 2Michael Smith Laboratories, Department of Microbiology & Immunology, University of British 12 Columbia, Vancouver, British Columbia, Canada 13 3Department of Physiology & Pharmacology, University of Calgary, Calgary, Alberta, Canada 14 4Department of Pediatrics, University of Calgary, Calgary, Alberta, Canada 15 5Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada 16 6Department of Botany, University
    [Show full text]
  • Combination Anthelmintic Treatment for Persistent Ancylostoma Caninum Ova Shedding in Greyhounds
    CASE SERIES Combination Anthelmintic Treatment for Persistent Ancylostoma caninum Ova Shedding in Greyhounds Lindie B. Hess, BS, Laurie M. Millward, DVM, Adam Rudinsky DVM, Emily Vincent, BS, Antoinette Marsh, PhD ABSTRACT Ancylostoma caninum is a nematode of the canine gastrointestinal tract commonly referred to as hookworm. This study involved eight privately owned adult greyhounds presenting with persistent A. caninum ova shedding despite previous deworming treatments. The dogs received a combination treatment protocol comprising topical moxidectin, followed by pyrantel/febantel/praziquantel within 24 hr. At 7–10 days posttreatment, a fecal examination monitored for parasite ova. Dogs remained on the monthly combination treatment protocol until they ceased shedding detectable ova. The dogs then received only the monthly topical moxidectin maintenance treatment. The dogs remained in the study for 5–14 mo with periodical fecal examinations performed. During the study, three dogs reverted to positive fecal ova status, with two being associated with client noncompliance. Reinstitution of the combination treatment protocol resulted in no detectable ova. Use of monthly doses of combination pyrantel, febantel and moxidectin appears to be an effective treatment for nonresponsive or persistent A. caninum ova shedding. Follow-up fecal examinations were important for verifying the presence or absence of ova shedding despite the use of anthelmintic treatment. Limitations of the current study include small sample size, inclusion of only privately owned greyhounds, and client compliance with fecal collection and animal care. (JAmAnimHospAssoc2019; 55:---–---. DOI 10.5326/ JAAHA-MS-6904) Introduction include the following: moxidectina,b, milbemycin oximec, fenben- Ancylostoma caninum is a nematode of the canine gastrointestinal dazoled, and/or pyrantel-containing productse,f.
    [Show full text]
  • Entamoeba Histolytica
    Journal of Clinical Microbiology and Biochemical Technology Piotr Nowak1*, Katarzyna Mastalska1 Review Article and Jakub Loster2 1Laboratory of Parasitology, Department of Microbiology, University Hospital in Krakow, 19 Entamoeba Histolytica - Pathogenic Kopernika Street, 31-501 Krakow, Poland 2Department of Infectious Diseases, University Protozoan of the Large Intestine in Hospital in Krakow, 5 Sniadeckich Street, 31-531 Krakow, Poland Humans Dates: Received: 01 December, 2015; Accepted: 29 December, 2015; Published: 30 December, 2015 *Corresponding author: Piotr Nowak, Laboratory of Abstract Parasitology, Department of Microbiology, University Entamoeba histolytica is a cosmopolitan, parasitic protozoan of human large intestine, which is Hospital in Krakow, 19 Kopernika Street, 31- 501 a causative agent of amoebiasis. Amoebiasis manifests with persistent diarrhea containing mucus Krakow, Poland, Tel: +4812/4247587; Fax: +4812/ or blood, accompanied by abdominal pain, flatulence, nausea and fever. In some cases amoebas 4247581; E-mail: may travel through the bloodstream from the intestine to the liver or to other organs, causing multiple www.peertechz.com abscesses. Amoebiasis is a dangerous, parasitic disease and after malaria the second cause of deaths related to parasitic infections worldwide. The highest rate of infections is observed among people living Keywords: Entamoeba histolytica; Entamoeba in or traveling through the tropics. Laboratory diagnosis of amoebiasis is quite difficult, comprising dispar; Entamoeba moshkovskii; Entamoeba of microscopy and methods of molecular biology. Pathogenic species Entamoeba histolytica has to histolytica sensu lato; Entamoeba histolytica sensu be differentiated from other nonpathogenic amoebas of the intestine, so called commensals, that stricto; commensals of the large intestine; amoebiasis very often live in the human large intestine and remain harmless.
    [Show full text]
  • Volvox Barberi Flocks, Forming Near-Optimal, Two
    bioRxiv preprint doi: https://doi.org/10.1101/279059; this version posted March 8, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Volvox barberi flocks, forming near-optimal, two-dimensional, polydisperse lattice packings Ravi Nicholas Balasubramanian1 1Harriton High School, 600 North Ithan Avenue, Bryn Mawr, PA 19010, USA Volvox barberi is a multicellular green alga forming spherical colonies of 10000-50000 differentiated somatic and germ cells. Here, I show that these colonies actively self-organize over minutes into “flocks" that can contain more than 100 colonies moving and rotating collectively for hours. The colonies in flocks form two-dimensional, irregular, \active crystals", with lattice angles and colony diameters both following log-normal distributions. Comparison with a dynamical simulation of soft spheres with diameters matched to the Volvox samples, and a weak long-range attractive force, show that the Volvox flocks achieve optimal random close-packing. A dye tracer in the Volvox medium revealed large hydrodynamic vortices generated by colony and flock rotations, providing a likely source of the forces leading to flocking and optimal packing. INTRODUCTION behavior (see, e.g., [8, 9] and references therein) but their interactions are often dominated by viscous forces (e.g. fluid drag) unlike larger organisms which are The remarkable multicellular green alga Volvox barberi dominated by inertial forces. Here, I show that V. [1] forms spherical colonies of 10,000 to 50,000 cells barberi colonies, which are themselves composed of many embedded in a glycol-protein based extra cellular matrix individual cells acting together, show collective behavior (ECM) and connected by cytoplasmic bridges that may at a higher level of organization.
    [Show full text]
  • Characterisation of Cryptosporidium Growth And
    CHARACTERISATION OF CRYPTOSPORIDIUM GROWTH AND PROPAGATION IN CELL FREE ENVIRONMENTS Annika Estcourt BSc (Hons) This thesis is presented for the degree of Doctor of Philosophy at Murdoch University 2011 1 DECLARATION I declare that this thesis is my own account of my research and contains as its main content, work that has not previously been submitted for a degree at any tertiary institution. Annika Estcourt (previously Boxell) 2 ACKNOWLEDGEMENTS Well finally! 8 years, almost to the day, from the very start of commencing on this journey, here it is, the finished product complete with blood, sweat and tears! Many times I have imagined the feeling of finally handing in and the celebrations I would have with family and friends when my PhD came to fruition. If I had a dollar for every time one of my friends, family or work colleagues asked me over the last four years ‘how is your thesis going?’ or ‘have you handed in yet?’ I would be very wealthy! As much as these questions occasionally hit a raw nerve, I would like to thank every one of you who kept on me as this has driven me bit by bit to put the finishing touches on my thesis in amidst concentrating on my new career path and being side tracked with life’s up and downs and my favorite hobby – horses! My sincere gratitude goes to my supervisors who have never given up on me! First and foremost, my heartfelt appreciation to Prof. Una Ryan. Una, you have been the most incredible supervisor anyone could ever have or wish for.
    [Show full text]
  • Introduction to the Cell Cell History Cell Structures and Functions
    Introduction to the cell cell history cell structures and functions CK-12 Foundation December 16, 2009 CK-12 Foundation is a non-profit organization with a mission to reduce the cost of textbook materials for the K-12 market both in the U.S. and worldwide. Using an open-content, web-based collaborative model termed the “FlexBook,” CK-12 intends to pioneer the generation and distribution of high quality educational content that will serve both as core text as well as provide an adaptive environment for learning. Copyright ©2009 CK-12 Foundation This work is licensed under the Creative Commons Attribution-Share Alike 3.0 United States License. To view a copy of this license, visit http://creativecommons.org/licenses/by-sa/3.0/us/ or send a letter to Creative Commons, 171 Second Street, Suite 300, San Francisco, California, 94105, USA. Contents 1 Cell structure and function dec 16 5 1.1 Lesson 3.1: Introduction to Cells .................................. 5 3 www.ck12.org www.ck12.org 4 Chapter 1 Cell structure and function dec 16 1.1 Lesson 3.1: Introduction to Cells Lesson Objectives • Identify the scientists that first observed cells. • Outline the importance of microscopes in the discovery of cells. • Summarize what the cell theory proposes. • Identify the limitations on cell size. • Identify the four parts common to all cells. • Compare prokaryotic and eukaryotic cells. Introduction Knowing the make up of cells and how cells work is necessary to all of the biological sciences. Learning about the similarities and differences between cell types is particularly important to the fields of cell biology and molecular biology.
    [Show full text]
  • Algae of the Genus Volvox (Chlorophyta) in Sub-Extreme Habitats T A.G
    Short Communication T REPRO N DU The International Journal of Plant Reproductive Biology 12(2) July, 2020, pp.156-158 LA C P T I F V O E B Y T I DOI 10.14787/ijprb.2020 12.2. O E I L O C G O S I S T E S H Algae of the genus Volvox (Chlorophyta) in sub-extreme habitats T A.G. Desnitskiy Department of Embryology, Saint-Petersburg State University, Saint-Petersburg, 199034, Universitetskaya nab. 7/9, Russia e-mail: [email protected]; [email protected] Received: 18. 05. 2020; Revised: 08. 06. 2020; Accepted and Published online: 15. 06. 2020 ABSTRACT Literature data on the life of green colonial algae of the genus Volvox (Chlorophyta) in sub-extreme habitats (polar, sub-polar and mountain regions) are critically considered. Very few species (primarily homothallic Volvox aureus) are able to thrive in such conditions. Keywords : Geographical distribution, reproduction, sub-extreme habitats, Volvox. The genus Volvox Linnaeus (Volvocaceae, Chlorophyta) Peru (South America) at the elevation of more than five includes more than 20 species of freshwater flagellate algae thousand meters above sea level seems to be doubtful. The (Nozaki et al. 2015), providing an opportunity to study the illustration from this article (which focuses mainly on developmental mechanisms in a relatively simple system diatoms) shows a spherical colony with a diameter of about 14 consisting of two cellular types (somatic and reproductive). μm, consisting of several hundred very small cells (Fritz et al. Volvox carteri f. nagariensis Iyengar is a valuable model of 2015, p.
    [Show full text]
  • The Intestinal Protozoa
    The Intestinal Protozoa A. Introduction 1. The Phylum Protozoa is classified into four major subdivisions according to the methods of locomotion and reproduction. a. The amoebae (Superclass Sarcodina, Class Rhizopodea move by means of pseudopodia and reproduce exclusively by asexual binary division. b. The flagellates (Superclass Mastigophora, Class Zoomasitgophorea) typically move by long, whiplike flagella and reproduce by binary fission. c. The ciliates (Subphylum Ciliophora, Class Ciliata) are propelled by rows of cilia that beat with a synchronized wavelike motion. d. The sporozoans (Subphylum Sporozoa) lack specialized organelles of motility but have a unique type of life cycle, alternating between sexual and asexual reproductive cycles (alternation of generations). e. Number of species - there are about 45,000 protozoan species; around 8000 are parasitic, and around 25 species are important to humans. 2. Diagnosis - must learn to differentiate between the harmless and the medically important. This is most often based upon the morphology of respective organisms. 3. Transmission - mostly person-to-person, via fecal-oral route; fecally contaminated food or water important (organisms remain viable for around 30 days in cool moist environment with few bacteria; other means of transmission include sexual, insects, animals (zoonoses). B. Structures 1. trophozoite - the motile vegetative stage; multiplies via binary fission; colonizes host. 2. cyst - the inactive, non-motile, infective stage; survives the environment due to the presence of a cyst wall. 3. nuclear structure - important in the identification of organisms and species differentiation. 4. diagnostic features a. size - helpful in identifying organisms; must have calibrated objectives on the microscope in order to measure accurately.
    [Show full text]
  • Lecture 5: Emerging Parasitic Helminths Part 2: Tissue Nematodes
    Readings-Nematodes • Ch. 11 (pp. 290, 291-93, 295 [box 11.1], 304 [box 11.2]) • Lecture 5: Emerging Parasitic Ch.14 (p. 375, 367 [table 14.1]) Helminths part 2: Tissue Nematodes Matt Tucker, M.S., MSPH [email protected] HSC4933 Emerging Infectious Diseases HSC4933. Emerging Infectious Diseases 2 Monsters Inside Me Learning Objectives • Toxocariasis, larva migrans (Toxocara canis, dog hookworm): • Understand how visceral larval migrans, cutaneous larval migrans, and ocular larval migrans can occur Background: • Know basic attributes of tissue nematodes and be able to distinguish http://animal.discovery.com/invertebrates/monsters-inside- these nematodes from each other and also from other types of me/toxocariasis-toxocara-roundworm/ nematodes • Understand life cycles of tissue nematodes, noting similarities and Videos: http://animal.discovery.com/videos/monsters-inside- significant difference me-toxocariasis.html • Know infective stages, various hosts involved in a particular cycle • Be familiar with diagnostic criteria, epidemiology, pathogenicity, http://animal.discovery.com/videos/monsters-inside-me- &treatment toxocara-parasite.html • Identify locations in world where certain parasites exist • Note drugs (always available) that are used to treat parasites • Describe factors of tissue nematodes that can make them emerging infectious diseases • Be familiar with Dracunculiasis and status of eradication HSC4933. Emerging Infectious Diseases 3 HSC4933. Emerging Infectious Diseases 4 Lecture 5: On the Menu Problems with other hookworms • Cutaneous larva migrans or Visceral Tissue Nematodes larva migrans • Hookworms of other animals • Cutaneous Larva Migrans frequently fail to penetrate the human dermis (and beyond). • Visceral Larva Migrans – Ancylostoma braziliense (most common- in Gulf Coast and tropics), • Gnathostoma spp. Ancylostoma caninum, Ancylostoma “creeping eruption” ceylanicum, • Trichinella spiralis • They migrate through the epidermis leaving typical tracks • Dracunculus medinensis • Eosinophilic enteritis-emerging problem in Australia HSC4933.
    [Show full text]
  • Parasites and Wildlife 10 (2019) 87–92
    IJP: Parasites and Wildlife 10 (2019) 87–92 Contents lists available at ScienceDirect IJP: Parasites and Wildlife journal homepage: www.elsevier.com/locate/ijppaw Molecular survey on the occurrence of avian haemosporidia, Coxiella burnetii and Francisella tularensis in waterfowl from central Italy T Valentina Virginia Ebania,*, Simona Nardonia, Marinella Giania, Guido Rocchigiania, Talieh Archinb, Iolanda Altomontea, Alessandro Polia, Francesca Manciantia a Department of Veterinary Science, University of Pisa, viale delle Piagge 2, 56124, Pisa, Italy b Department of Microbiology, College of Veterinary Medicine, Urmia University, Urmia, Iran ARTICLE INFO ABSTRACT Keywords: The aim of the present study was to evaluate the occurrence of some avian Haemosporidia, Coxiella burnetii and Waterfowl Francisella tularensis in waterfowl from Tuscany wetlands. One-hundred and thirty-three samples of spleen were Leucocytozoon spp. collected from regularly hunted wild birds belonging to 13 different waterfowl species. DNA extracted from each Plasmodium spp sample was submitted to PCR assays and sequencing to detect the pathogens. Thirty-three samples (24.81%) Haemoproteus spp were positive with PCR for at least one pathogen: 23 (17.29%) for Leucocytozoon spp., 6 (4.51%) for Plasmodium Coxiella burnetii spp., 4 (3%) for C. burnetii, 2 (1.5%) for Haemoproteus spp. No specific F. tularensis amplifications (0%) were Francisella tularensis detected. To the best of our knowledge, this study firstly reports data about haemosporidian and C. burnetii infections in waterfowl from Italy. 1. Introduction waterfowl by microscopy in Italy yielded no positive results. Coxiella burnetii is the etiologic agent of Q fever, a worldwide zoo- Avian haemosporidia are a group of protozoan parasites, among notic bacterial disease.
    [Show full text]
  • Visceral and Cutaneous Larva Migrans PAUL C
    Visceral and Cutaneous Larva Migrans PAUL C. BEAVER, Ph.D. AMONG ANIMALS in general there is a In the development of our concepts of larva II. wide variety of parasitic infections in migrans there have been four major steps. The which larval stages migrate through and some¬ first, of course, was the discovery by Kirby- times later reside in the tissues of the host with¬ Smith and his associates some 30 years ago of out developing into fully mature adults. When nematode larvae in the skin of patients with such parasites are found in human hosts, the creeping eruption in Jacksonville, Fla. (6). infection may be referred to as larva migrans This was followed immediately by experi¬ although definition of this term is becoming mental proof by numerous workers that the increasingly difficult. The organisms impli¬ larvae of A. braziliense readily penetrate the cated in infections of this type include certain human skin and produce severe, typical creep¬ species of arthropods, flatworms, and nema¬ ing eruption. todes, but more especially the nematodes. From a practical point of view these demon¬ As generally used, the term larva migrans strations were perhaps too conclusive in that refers particularly to the migration of dog and they encouraged the impression that A. brazil¬ cat hookworm larvae in the human skin (cu¬ iense was the only cause of creeping eruption, taneous larva migrans or creeping eruption) and detracted from equally conclusive demon¬ and the migration of dog and cat ascarids in strations that other species of nematode larvae the viscera (visceral larva migrans). In a still have the ability to produce similarly the pro¬ more restricted sense, the terms cutaneous larva gressive linear lesions characteristic of creep¬ migrans and visceral larva migrans are some¬ ing eruption.
    [Show full text]