DOCSLIB.ORG

Identification of Intestinal Microbes in Children With

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Identification of Intestinal Microbes in Children With IDENTIFICATION OF INTESTINAL MICROBES IN CHILDREN WITH DIARRHEA ANDNON-DIARRHEA USING POLYMERASE CHAIN REACTION / ELECTROSPRAY IONIZATION-MASS SPECTROMETRY (PCR / ESI-MS) Teguh Sarry Hartono1, Dewi Murniati2, Andi Yasmon3, Lucky H Moehario3 1 Infectious Disease HospitalProf Dr Sulianti Saroso, Microbiology Resident–Departement of Microbiology, Faculty of Medicine, University of Indonesia. 2Infectious Disease Hospital Prof Dr Sulianti Saroso, 3Department of Microbiology, Faculty of Medicine, University of Indonesia. Abstract :Microbiota present in human intestinal are diverse, and imbalance in composition of intestinal flora may cause diarrhea.This study aimed to obtain a profile of intestinal bacteria in children with and without diarrhea and assess their presence with incidence of diarrhea. An analitical descriptive with cross sectional design study was carried out. A stool specimen was collected from each children of 2-12 years old with and without diarrhea who lived in North Jakarta. DNA extraction was performed prior to detection of microbes using Polymerase Chain Ceaction/Electrospray Ionization-Mass Spectrometry. Eighty stool specimens consisted of 33 and 47 specimens from children with and without diarrhea were included in the study. Thirty single and 6 multiple matches were detected in 30 specimens of the diarrhea group; 28 single and 8 multiple matches were found in 34 specimens of the non-diarrhea.Escherechiacoli and Klebsiella pneumonia were predominant in both groups. Firmicutes, Proteobacteria and Bacteroidetes were deteced in the diarrhea group, while Actinobacteria, Proteobacteria and Verrucomicrobia were in the non-diarrhea. The relationship of incidence of diarrhea and the present of enteropathogens in the stool was not significant, however, there was a strong correlation of the risk of suffering diarrhea due to the presence of enteropathogens (OR = 0.724 with 95%, CI: 0.237-2.215). In conclusion, most bacteria detected in both groups were similar, nonetheless, Actinobacteria was present only in the non-diarrhea. The chance to have diarrhea was higher when enteropathogen was detected in the stool. Keywords:Gut microbiota, diarrhea, PCR/ESI-MS Correspondence : were more prevalent4.Infection will affect dr. Teguh Sarry Hartono, Sp.MK on interaction among microbiota and Infectious Disease Hospital Prof Dr Sulianti trigger inflammation responses, and Saroso, Jalan Baru Sunter Permai Raya, consequently influence the composition of Jakarta 14350 intestinal microbiota5. Lupp C et al, 2007 Email : [email protected] reported that intestinal mucosal inflammatory process due to infection INTRODUCTION altered the residentobligate anaerobic Gastrointestinaltract (GIT) is themost bacteria and triggered excessive growth of heavily colonized organ; the colon alone commensal Enterobacteriaceae to 5 contains over 70% of all the microbes in become pathogenic . the human body1,2.The intestinal A variety of approacheswhich include 6 microbiota composed of more than 1000 full-length 16S rRNA sequencing , species3. Almost all of these species denaturing gradient gel electrophoresis by 7 (98%) belonged to only four bacterial Zoetendal et al , and fluorescent insitu 8 phyla i.e. Firmicutes (64%), Bacteroidetes hybridization by Franks et al had been (23%), Proteobacteria (8%), and used in the study of intestinal microbial Actinobacteria (3%)4.Other species such communities. Further, Polymerase Chain as members of Verrumicrobia, Reaction coupled with Electrospray Fusobacteria and Cyanobacteria exist in Ionization/Mass Spectrometry (PCR/ESI- small amount3. Microbes in small intestine MS), a technology based on PCR were enriched with the Bacilliclass of technique, and dispersion technology of Firmicutes and Actinobacteria, while in electro ionization mass spectrometry, has colon, Bacteroidetes and the been utilized for a complete identification 9,10 Lachnospiraceae, family of Firmicutes of a largenumber of microorganisms . It The Indonesian Journal of Infectious Disease 1 has a capability to identy multiple PCR-ESI/MS assay organism present in a sample up to Prior to PCR-ESI/MS, inhibitor in the species level simultaneously without prior samples were analized by conventional cultivication9,11,12. PCR. PCR-ESI/MS was performed by In this study we employed the using PLEX-ID Broad Bacteria panel in PCR/ESI-MS to identify intestinal accordance with manufacture’s instruction. microbes from stools of children aged 2-12 The data treshold is 0.85 confidence, in years with and without diarrhea in Jakarta, that less than 0.85 were reported and analyse their relationship to incidence uninterpratable14. of diarrhea. Such information will be valuablefor the development of Statistical analysis management of diarrhea in the context of Statistical analysis was performed by intestinal microbial balance. using SPSS ver 17 software. The relationship between entero pathogens and diarrhea cases was done using Chi- MATERIALS AND METHODS square test with a P-value of < 0.05 was considered significant. The risk Clinical specimens relationship (Odds ratio) between those The research was an analitical two was also measured. descriptive study with cross sectional design. Stool samples were collected from RESULT September 2012 to December 2012. Diarrhea samples were collected from out 1. Subjects profiles patients in Infectious Diseases Hospital Overall 80 subjects was obtained in (IDH) Prof Dr Sulianti Saroso and primary accordance with the inclusion criteria, health centers highlighted the close consisted of 33 children who had diarrhea relation area of study population in North (diarrhea subjects) and 47 children who Jakarta, Indonesia. Non diarrhea samples did not have diarrhea (non-diarrheal were collected from healthy children in subjects). Diarrhea subjects consisted of toddler classes and kindergartens in the 24 boys and 9 girls with an age range of 2 same area as mentioned above. The years to 9 years and the median age of 3 0 samples were stored at 4 C for not more years. While the non-diarrheal subjects than 24 hours and immediately consisted of 27 boys and 20 girls with an transported to laboratory using special age range of 2 years 3 months to 11 years 0 coolbox. All samples were stored at -70 C with a median age of 4 years and 4 until further process. Inclusion criteria for months. diarrhea sample were children aged 2-12 year which had an episode of acute 2. PLEX-ID result 13 diarrhea according to WHO criteria and PLEX-ID psitive detection means that has not received antibiotic.For non- the processor PLEX-ID reads one or more diarrhea sample,were children aged 2-12 microorganisms with a Q score > 0.85 in years, without episodes of diarrhea.This accordance with the spectrometer study was approved by Ethical Committee database PLEX-ID. If more than one of Faculty of Medicine, University of bacteria detected in one sample with a Indonesia. different Q score, means that there is more than one bacteria that has the same DNA extraction opportunities as the detected bacteria. Total DNA was extracted from 180-220 PLEX-ID detection would give three mg solid stool or 200 µl liquid stool. The kindsof result,as showedin Figure 1. extraction was performed by using Of 33 samples in diarrhea QIAamp® DNA Stool Mini Kit according to groupshowed the presence of 30 single the manufacturer's instructions with 200 µl matchbacteria and 6 multiple matches of the elution. The elution was stored at - bacteria, while the 3 other samples 0 70 C until processed. showed no detectable bacteria. In the non- diarrhea group (47 samples) showed 28 2 The Indonesian Journal of Infectious Disease single bacteria, 8 multiple matches and all cases with enteropathogens, 48.2% 13 samples showed no detectable (27/56) had diarrhea. While in the cases bacteria. with non-enteropathogens, which had diarrhea was 56.5% (9/16). Using Chi- 3. Detection of bacteria in samples square test gave P= 0.571. It means there Table 2 shows Escherechia coli is no significant relationship between dominated the diarrhea group, followed by enteropathogens with the incidence of Klebsiella pneumonia, both from the diarrhea.Odds ratio is used to see the phylum Proteobacteria. Another species strength of the relationship between the were Campylobacter jejuni, Acidovorax incidentof diarrhea by enteropathogens avenae and Aquaspirillum gracile, all from (OR 0,724 [95% CI: 0,237-2,215]). This Proteobacteria, while Staphylococcus means that the chance of having diarrhea epidermidis, Clostridium perfringens, caused by enteropathogens was 0.724 Streptococcus vestibularfrom Firmicutes fold than by non-enteropathogen. and Prevotella albensisfrom Bacteroidetes. DISCUSSION The non-diarrhea group also dominated by Echerechia coliand Klebsiella This study was conducted in four health pneumonia. Another Protobacteria centers and one hospital in North Jakarta. detected was Escherichia fergusonii. The health centers were selected on the Additionally it also detected the presence basis of the distance between the sites, of Actinobacteria (Bifidobacterium longum) which ranges from 4 km to IDH, to and Verrucomicrobia (Akkermansia facilitate sample collection. In addition, it muciniphila). was assumed homogenization of social, The diversity ofbacteria detected in the economic, environmental and climate of diarrhea group (12 of 30 samples) was the subjects. Specimens obtained were more than in non-diarrheal group (5 of placed in a cooler box with temperatures 0 28).Figure 2 shows Firmicutes and around 8 C before transfer to a
Load more

Recommended publications
  • Bacterial Communities of the Upper Respiratory Tract of Turkeys
    www.nature.com/scientificreports OPEN Bacterial communities of the upper respiratory tract of turkeys Olimpia Kursa1*, Grzegorz Tomczyk1, Anna Sawicka‑Durkalec1, Aleksandra Giza2 & Magdalena Słomiany‑Szwarc2 The respiratory tracts of turkeys play important roles in the overall health and performance of the birds. Understanding the bacterial communities present in the respiratory tracts of turkeys can be helpful to better understand the interactions between commensal or symbiotic microorganisms and other pathogenic bacteria or viral infections. The aim of this study was the characterization of the bacterial communities of upper respiratory tracks in commercial turkeys using NGS sequencing by the amplifcation of 16S rRNA gene with primers designed for hypervariable regions V3 and V4 (MiSeq, Illumina). From 10 phyla identifed in upper respiratory tract in turkeys, the most dominated phyla were Firmicutes and Proteobacteria. Diferences in composition of bacterial diversity were found at the family and genus level. At the genus level, the turkey sequences present in respiratory tract represent 144 established bacteria. Several respiratory pathogens that contribute to the development of infections in the respiratory system of birds were identifed, including the presence of Ornithobacterium and Mycoplasma OTUs. These results obtained in this study supply information about bacterial composition and diversity of the turkey upper respiratory tract. Knowledge about bacteria present in the respiratory tract and the roles they can play in infections can be useful in controlling, diagnosing and treating commercial turkey focks. Next-generation sequencing has resulted in a marked increase in culture-independent studies characterizing the microbiome of humans and animals1–6. Much of these works have been focused on the gut microbiome of humans and other production animals 7–11.
    [Show full text]
  • Purification and Characterization of a Shigella Dysenteriae 1- Like Toxin Produced by Escherichia Coli
    CORE Metadata, citation and similar papers at core.ac.uk Provided by UNL | Libraries University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Uniformed Services University of the Health Sciences U.S. Department of Defense 1983 Purification and Characterization of a Shigella dysenteriae 1- Like Toxin Produced by Escherichia coli Alison D. O'Brien Uniformed Services University of the Health Sciences, [email protected] Gerald D. LaVeck Uniformed Services University of the Health Sciences Follow this and additional works at: https://digitalcommons.unl.edu/usuhs Part of the Medicine and Health Sciences Commons O'Brien, Alison D. and LaVeck, Gerald D., "Purification and Characterization of a Shigella dysenteriae 1- Like Toxin Produced by Escherichia coli" (1983). Uniformed Services University of the Health Sciences. 99. https://digitalcommons.unl.edu/usuhs/99 This Article is brought to you for free and open access by the U.S. Department of Defense at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Uniformed Services University of the Health Sciences by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. INFECTION AND IMMUNITY, May 1983, p. 675-683 Vol. 40, No. 2 0019-9567/83/050675-09$02.00/0 Copyright C 1983, American Society for Microbiology Purification and Characterization of a Shigella dysenteriae 1- Like Toxin Produced by Escherichia coli ALISON D. O'BRIEN* AND GERALD D. LAVECKt Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 Received 10 January 1983/Accepted 18 February 1983 A toxin from an enteropathogenic strain of Escherichia coli (E. coli H30) was purified to apparent homogeneity from cell lysates.
    [Show full text]
  • Distribution of Bacterial 1,3-Galactosyltransferase Genes In
    Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut Microbiome Emmanuel Montassier, Gabriel Al-Ghalith, Camille Mathé, Quentin Le Bastard, Venceslas Douillard, Abel Garnier, Rémi Guimon, Bastien Raimondeau, Yann Touchefeu, Emilie Duchalais, et al. To cite this version: Emmanuel Montassier, Gabriel Al-Ghalith, Camille Mathé, Quentin Le Bastard, Venceslas Douillard, et al.. Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut Microbiome. Frontiers in Immunology, Frontiers, 2020, 10, pp.3000. 10.3389/fimmu.2019.03000. inserm-02490517 HAL Id: inserm-02490517 https://www.hal.inserm.fr/inserm-02490517 Submitted on 25 Feb 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. ORIGINAL RESEARCH published: 13 January 2020 doi: 10.3389/fimmu.2019.03000 Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut Microbiome Emmanuel Montassier 1,2,3, Gabriel A. Al-Ghalith 4, Camille Mathé 5,6, Quentin Le Bastard 1,3, Venceslas Douillard 5,6,7, Abel Garnier 5,6,7, Rémi Guimon 5,6,7, Bastien Raimondeau 5,6, Yann Touchefeu 8,9, Emilie Duchalais 8,9, Nicolas Vince 5,6, Sophie Limou 5,6, Pierre-Antoine Gourraud 5,6,7, David A.
    [Show full text]
  • Longitudinal Characterization of the Gut Bacterial and Fungal Communities in Yaks
    Journal of Fungi Article Longitudinal Characterization of the Gut Bacterial and Fungal Communities in Yaks Yaping Wang 1,2,3, Yuhang Fu 3, Yuanyuan He 3, Muhammad Fakhar-e-Alam Kulyar 3 , Mudassar Iqbal 3,4, Kun Li 1,2,* and Jiaguo Liu 1,2,* 1 Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; [email protected] 2 MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China 3 College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; [email protected] (Y.F.); [email protected] (Y.H.); [email protected] (M.F.-e.-A.K.); [email protected] (M.I.) 4 Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan * Correspondence: [email protected] (K.L.); [email protected] (J.L.) Abstract: Development phases are important in maturing immune systems, intestinal functions, and metabolism for the construction, structure, and diversity of microbiome in the intestine during the entire life. Characterizing the gut microbiota colonization and succession based on age-dependent effects might be crucial if a microbiota-based therapeutic or disease prevention strategy is adopted. The purpose of this study was to reveal the dynamic distribution of intestinal bacterial and fungal communities across all development stages in yaks. Dynamic changes (a substantial difference) in the structure and composition ratio of the microbial community were observed in yaks that Citation: Wang, Y.; Fu, Y.; He, Y.; matched the natural aging process from juvenile to natural aging.
    [Show full text]
  • Escherichia Fergusonii: a New Emerging Bacterial Disease of Farmed Nile Tilapia (Oreochromis Niloticus)
    Global Veterinaria 14 (2): 268-273, 2015 ISSN 1992-6197 © IDOSI Publications, 2015 DOI: 10.5829/idosi.gv.2015.14.02.9379 Escherichia fergusonii: A New Emerging Bacterial Disease of Farmed Nile Tilapia (Oreochromis niloticus) A.Y. Gaafar, A.M. Younes, A.M. Kenawy, W.S. Soliman and Laila A. Mohamed Department of Hydrobiology, National Research Centre (NRC), El-Bohooth Street (Formerly El-Tahrir St.) Dokki, Gizza 12622, Egypt Abstract: Tilapia is one of the most important cultured freshwater fish in Egypt. In June (2013) an unidentified bacterial disease outbreak occurred in earthen ponds raised Nile tilapia (Oreochromis niloticus). The API 20E test identified the strain as sorbitol-negative and positive for ADH, ornithine decarboxylase and amygdalin and provided the code 5144113, corresponding to E. fergusonii. The clinical signs of diseased fish showed emaciation, focal reddening of the skin at the base of the fins, exophthalmia with inflammation of periorbital area. Postmortem lesions showed enlargement of gallbladder and spleen with discolored hepatopancreas and congested posterior kidney. Lethal Dose fifty (LD50 ) was performed for experimental infection studies. Histopathological examination of naturally as well as experimentally infected specimens revealed pathological lesions in the hepatopancreas, posterior kidney, spleen, myocardium, skin and gills. The lesions ranged from circulatory, degenerative changes and mononuclear cells infiltrations to necrotic changes in the corresponding organs. As a conclusion, this study was the first evidence that E. fergusonii can infect fish especially farmed tilapia, causing considerable mortality and morbidity, in heavily manured earthen ponds. E. fergusonii causes significant pathological lesions in tilapia that are comparable to other susceptible terrestrial animals.
    [Show full text]
  • Insights Into the Pathogenicity of Burkholderia Pseudomallei
    REVIEWS Melioidosis: insights into the pathogenicity of Burkholderia pseudomallei W. Joost Wiersinga*, Tom van der Poll*, Nicholas J. White‡§, Nicholas P. Day‡§ and Sharon J. Peacock‡§ Abstract | Burkholderia pseudomallei is a potential bioterror agent and the causative agent of melioidosis, a severe disease that is endemic in areas of Southeast Asia and Northern Australia. Infection is often associated with bacterial dissemination to distant sites, and there are many possible disease manifestations, with melioidosis septic shock being the most severe. Eradication of the organism following infection is difficult, with a slow fever-clearance time, the need for prolonged antibiotic therapy and a high rate of relapse if therapy is not completed. Mortality from melioidosis septic shock remains high despite appropriate antimicrobial therapy. Prevention of disease and a reduction in mortality and the rate of relapse are priority areas for future research efforts. Studying how the disease is acquired and the host–pathogen interactions involved will underpin these efforts; this review presents an overview of current knowledge in these areas, highlighting key topics for evaluation. Melioidosis is a serious disease caused by the aerobic, rifamycins, colistin and aminoglycosides), but is usually Gram-negative soil-dwelling bacillus Burkholderia pseu- susceptible to amoxicillin-clavulanate, chloramphenicol, domallei and is most common in Southeast Asia and doxycycline, trimethoprim-sulphamethoxazole, ureido- Northern Australia. Melioidosis is responsible for 20% of penicillins, ceftazidime and carbapenems2,4. Treatment all community-acquired septicaemias and 40% of sepsis- is required for 20 weeks and is divided into intravenous related mortality in northeast Thailand. Reported cases are and oral phases2,4. Initial intravenous therapy is given likely to represent ‘the tip of the iceberg’1,2, as confirmation for 10–14 days; ceftazidime or a carbapenem are the of disease depends on bacterial isolation, a technique that drugs of choice.
    [Show full text]
  • Plesiomonas Shigelloides S000142446 Serratia Plymuthica
    0.01 Plesiomonas shigelloides S000142446 Serratia plymuthica S000016955 Serratia ficaria S000010445 Serratia entomophila S000015406 Leads to highlighted edge in Supplemental Phylogeny 1 Xenorhabdus hominickii S000735562 0.904 Xenorhabdus poinarii S000413914 0.998 0.868 Xenorhabdus griffiniae S000735553 Proteus myxofaciens S000728630 0.862 Proteus vulgaris S000004373 Proteus mirabilis S000728627 0.990 0.822 Arsenophonus nasoniae S000404964 OTU from Corby-Harris (Unpublished) #seqs-1 GQ988429 OTU from Corby-Harris et al (2007) #seqs-1 DQ980880 0.838 Providencia stuartii S000001277 Providencia rettgeri S000544654 0.999 Providencia vermicola S000544657 0.980 Isolate from Juneja and Lazzaro (2009)-EU587107 Isolate from Juneja and Lazzaro (2009)-EU587113 0.605 0.928 Isolate from Juneja and Lazzaro (2009)-EU587095 0.588 Isolate from Juneja and Lazzaro (2009)-EU587101 Providencia rustigianii S000544651 0.575 Providencia alcalifaciens S000127327 0.962 OTU XDS 099-#libs(1/0)-#seqs(1/0) OTU XYM 085-#libs(13/4)-#seqs(401/23) 0.620 OTU XDY 021-#libs(1/1)-#seqs(3/1) Providencia heimbachae S000544652 Morganella psychrotolerans S000721631 0.994 Morganella morganii S000721642 OTU ICF 062-#libs(0/1)-#seqs(0/7) Morganella morganii S000129416 0.990 Biostraticola tofi S000901778 0.783 Sodalis glossinidius S000436949 Dickeya dadantii S000570097 0.869 0.907 0.526 Brenneria rubrifaciens S000022162 0.229 Brenneria salicis S000381181 0.806 OTU SEC 066-#libs(0/1)-#seqs(0/1) Brenneria quercina S000005099 0.995 Pragia fontium S000022757 Budvicia aquatica S000020702
    [Show full text]
  • Use of the Diagnostic Bacteriology Laboratory: a Practical Review for the Clinician
    148 Postgrad Med J 2001;77:148–156 REVIEWS Postgrad Med J: first published as 10.1136/pmj.77.905.148 on 1 March 2001. Downloaded from Use of the diagnostic bacteriology laboratory: a practical review for the clinician W J Steinbach, A K Shetty Lucile Salter Packard Children’s Hospital at EVective utilisation and understanding of the Stanford, Stanford Box 1: Gram stain technique University School of clinical bacteriology laboratory can greatly aid Medicine, 725 Welch in the diagnosis of infectious diseases. Al- (1) Air dry specimen and fix with Road, Palo Alto, though described more than a century ago, the methanol or heat. California, USA 94304, Gram stain remains the most frequently used (2) Add crystal violet stain. USA rapid diagnostic test, and in conjunction with W J Steinbach various biochemical tests is the cornerstone of (3) Rinse with water to wash unbound A K Shetty the clinical laboratory. First described by Dan- dye, add mordant (for example, iodine: 12 potassium iodide). Correspondence to: ish pathologist Christian Gram in 1884 and Dr Steinbach later slightly modified, the Gram stain easily (4) After waiting 30–60 seconds, rinse with [email protected] divides bacteria into two groups, Gram positive water. Submitted 27 March 2000 and Gram negative, on the basis of their cell (5) Add decolorising solvent (ethanol or Accepted 5 June 2000 wall and cell membrane permeability to acetone) to remove unbound dye. Growth on artificial medium Obligate intracellular (6) Counterstain with safranin. Chlamydia Legionella Gram positive bacteria stain blue Coxiella Ehrlichia Rickettsia (retained crystal violet).
    [Show full text]
  • Sexually Transmitted Diseases Act)
    Europe’s journal on infectious disease epidemiology, prevention and control © Science Photo Special edition: Sexually transmitted infections August 2012 • Reports highlighting increasing trends of gonorrhoea and syphilis and the threat of drug-resistant gonorrhoea in Europe. www.eurosurveillance.org Editorial team Editorial advisors Based at the European Centre for Albania: Alban Ylli, Tirana Disease Prevention and Control (ECDC), Austria: Reinhild Strauss, Vienna 171 83 Stockholm, Sweden Belgium: Koen De Schrijver, Antwerp Telephone number Belgium: Sophie Quoilin, Brussels +46 (0)8 58 60 11 38 or +46 (0)8 58 60 11 36 Bosnia and Herzogovina: Nina Rodić Vukmir, Banja Luka Fax number Bulgaria: Mira Kojouharova, Sofia +46 (0)8 58 60 12 94 Croatia: TBC, Zagreb Cyprus: Chrystalla Hadjianastassiou, Nicosia E-mail Czech Republic: Bohumir Križ, Prague [email protected] Denmark: Peter Henrik Andersen, Copenhagen Editor-in-chief England and Wales: TBC, London Ines Steffens Estonia: Kuulo Kutsar, Tallinn Finland: Outi Lyytikäinen, Helsinki Scientific editors France: Judith Benrekassa, Paris Kathrin Hagmaier Germany: Jamela Seedat, Berlin Williamina Wilson Greece: Rengina Vorou, Athens Karen Wilson Hungary: Ágnes Csohán, Budapest Assistant editors Iceland: Haraldur Briem, Reykjavik Alina Buzdugan Ireland: Lelia Thornton, Dublin Ingela Söderlund Italy: Paola De Castro, Rome Associate editors Kosovo (under UNSCR 1244/99): Lul Raka, Pristina Andrea Ammon, Stockholm, Sweden Latvia: Jurijs Perevoščikovs, Riga Tommi Asikainen, Frankfurt, Germany
    [Show full text]
  • Shigellosis Infection with S
    lasting about a week. The tropical forms cause more severe illnesses. Symptoms usually last 2‐ 4 weeks. Shigellosis Infection with S. dysenteriae tends to be severe and (Notifiable) prolonged and requiring hospital admission. Toxic megacolon is occasionally seen in disease caused by S. Description: Shigellosis produces a classical dysenteriae Type I. Infection with S. flexneri can lead to gastroenteritis with pronounced, occasionally bloody, Reiter’s Syndrome (reactive post‐infectious diarrhoea. About one third of cases are foodborne. arthropathy). HUS is a recognised complication of bacillary dysentery (it is closely associated with infection Annual Numbers: Between 40 and 80 cases per year. due to S. dysenteriae Type I) and is more likely to develop if amoxicillin is given during the diarrhoeal Seasonal Distribution: There is no seasonal pattern of phase. The case fatality rate with S. dysenteriae Type I is incidence. between 20 and 40% even in developed countries. Causative Agent: The causative agents of shigellosis are Clinical Management of Cases Shigella sonnei, Shigella boydii, Shigella dysenteriae and Enteric precautions including hygiene advice. The case Shigella flexneri. S. sonnei and S. dysenteriae each should be notified to the local Department of Public account for about 40% of cases. About 40% of cases Health. It is important to determine if the case is aware report recent travel to Africa. Fewer than 10% of cases of similar cases suggesting the possibility of an outbreak. did not travel outside Ireland. Determine if case is in a risk category. Reservoir: The gastrointestinal tracts of humans and Public Health Management of Cases (occasionally) apes. Enteric precautions including hygiene advice.
    [Show full text]
  • Detection of Shigella Spp. and Enteroinvasive Escherichia Coli MALABI VENKATESAN,* JERRY M
    JOURNAL OF CLINICAL MICROBIOLOGY, Feb. 1988, p. 261-266 Vol. 26, No. 2 0095-1137/88/020261-06$02.00/0 Copyright ©D 1988, American Society for Microbiology Development and Testing of Invasion-Associated DNA Probes for Detection of Shigella spp. and Enteroinvasive Escherichia coli MALABI VENKATESAN,* JERRY M. BUYSSE, ERIC VANDENDRIES, AND DENNIS J. KOPECKO Department ofBacterial Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute ofResearch, Washington, D.C. 20307-5100 Received 29 June 1987/Accepted 29 October 1987 Genetic determinants of the invasive phenotype of Shigeila spp. and enteroinvasive Escherichia coli (EIEC), two common agents of bacillary dysentery, are encôded on large (180- to 210 kilobase), nonconjugative plasmids. Several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate the attachment and invasion of colonic epithelial cells by the bacteria. Selected invasion plasmid antigen (ipa) genes have recently been cloned from Shigellaflexneri serotype 5 into the X gtll expression vector. Portions of three ipa genes (ipaB, ipaC, and ipaD) were tested as DNA probes for diagnostic detection of bacillary dysentery. Undér stringent DNA hybridization conditions, all three DNA sequences hybridized to a single 4.6-kilobase HindIII fragment of the invasion plasmids of representative virulent Shigella spp. and EIEC strains. No hybridization was detected in isogenic, noninvasive Shigella mutants which had lost the invasion plasmid or had deleted the ipa gene region. Furthermore, these probes did not react with over 300 other enteric and nonenteric gram-negative bacteria tested, including Salmonella, Yersinia, Edwardsiella, Campylobacter, Vibrio, Klebsiella, Aeromonas, Enterobacter, Rickettsia, and Citrobacter spp. and various pathogenic E.
    [Show full text]
  • United States Patent (10) Patent No.: US 7820,184 B2 Stritzker Et Al
    USOO782O184B2 (12) United States Patent (10) Patent No.: US 7820,184 B2 Stritzker et al. (45) Date of Patent: Oct. 26, 2010 (54) METHODS AND COMPOSITIONS FOR 5,833,975 A 1 1/1998 Paoletti et al. ............. 424.93.2 DETECTION OF MICROORGANISMS AND 5,976,796. A 1 1/1999 Szalay et al. ................... 435/6 SirNRTREATMENT OF DISEASES AND 6,025,155 A 2/2000 Hadlaczky et al. ......... 435/69.1 6,045,802 A 4/2000 Schlom et al. ........... 424,199.1 (75) Inventors: Jochen Harald Stritzker, Kissing (DE); 6,077,697 A 6/2000 Hadlaczky et al. 435/1723 Phil Hill, West Bridgford (GB); Aladar 6,080,849 A 6/2000 Bermudes et al. .......... 536,23.7 A. Szalay, Highland, CA (US); Yong A. 6,093,700 A 7/2000 Mastrangelo et al. ......... 514,44 Yu, San Diego, CA (US) 6,099,848. A 8/2000 Frankel et al. ........... 424,246.1 6,106,826 A 8/2000 Brandt et al. .............. 424.93.2 (73) Assignee: stylus Corporation, San Diego, CA 6, 190,657 B1 2/2001 Pawelek et al. ............ 424,931 6,217,847 B1 4/2001 Contaget al. ................ 4249.1 (*) Notice: Subject to any disclaimer, the term of this 6,232,523 B1 5/2001 Tan et al. ...................... 800, 10 patent is extended or adjusted under 35 6,235,967 B1 5/2001 Tan et al. ...................... 800, 10 U.S.C. 154(b) by 362 days. 6,235,968 B1 5/2001 Tan et al. ...................... 800, 10 6,251,384 B1 6/2001 Tan et al.
    [Show full text]