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United States Patent (10) Patent No.: US 7820,184 B2 Stritzker Et Al

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United States Patent (10) Patent No.: US 7820,184 B2 Stritzker Et Al USOO782O184B2 (12) United States Patent (10) Patent No.: US 7820,184 B2 Stritzker et al. (45) Date of Patent: Oct. 26, 2010 (54) METHODS AND COMPOSITIONS FOR 5,833,975 A 1 1/1998 Paoletti et al. ............. 424.93.2 DETECTION OF MICROORGANISMS AND 5,976,796. A 1 1/1999 Szalay et al. ................... 435/6 SirNRTREATMENT OF DISEASES AND 6,025,155 A 2/2000 Hadlaczky et al. ......... 435/69.1 6,045,802 A 4/2000 Schlom et al. ........... 424,199.1 (75) Inventors: Jochen Harald Stritzker, Kissing (DE); 6,077,697 A 6/2000 Hadlaczky et al. 435/1723 Phil Hill, West Bridgford (GB); Aladar 6,080,849 A 6/2000 Bermudes et al. .......... 536,23.7 A. Szalay, Highland, CA (US); Yong A. 6,093,700 A 7/2000 Mastrangelo et al. ......... 514,44 Yu, San Diego, CA (US) 6,099,848. A 8/2000 Frankel et al. ........... 424,246.1 6,106,826 A 8/2000 Brandt et al. .............. 424.93.2 (73) Assignee: stylus Corporation, San Diego, CA 6, 190,657 B1 2/2001 Pawelek et al. ............ 424,931 6,217,847 B1 4/2001 Contaget al. ................ 4249.1 (*) Notice: Subject to any disclaimer, the term of this 6,232,523 B1 5/2001 Tan et al. ...................... 800, 10 patent is extended or adjusted under 35 6,235,967 B1 5/2001 Tan et al. ...................... 800, 10 U.S.C. 154(b) by 362 days. 6,235,968 B1 5/2001 Tan et al. ...................... 800, 10 6,251,384 B1 6/2001 Tan et al. ....... ... 424/93.21 (21) Appl. No.: 12/008,659 6,416,754 B1 7/2002 Brown et al. ...... ... 424/93.21 6,447,784 B1 9, 2002 Bermudes et al. ........ 424/235.1 (22) Filed: Jan. 11, 2008 6,491.905 B1 12/2002 Sorscher et al. ............. 435/325 (65) Prior Publication Data 6,503,703 B1 1/2003 Palese et al. ................... 435/5 f 1 6,511,967 B1 1/2003 Weissleder et al. ............ 514,44 US 2009/O180987 A1 Jul. 16, 2009 6,548,068 B1 4/2003 Schlom et al. ........... 424,199.1 Related U.S. Application Data (63) Continuation of application No. 1 1/827,518, filed on Jul. 11, 2007. (Continued) (60) Provisional application No. 60/852,389, filed on Oct. FOREIGN PATENT DOCUMENTS 16, 2006, provisional application No. 60/830,422, filed on Jul. 11, 2006. DE 102 O9958 11, 2006 (51) Int. Cl. A6 IK 39/08 (2006.01) A6 IK 49/00 (2006.01) (Continued) CI2O I/00 (2006.01) OTHER PUBLICATIONS (52) U.S. Cl. ...................... 424/241.1; 424/9.1; 424/9.2: 424/234.1; 435/4; 435/8; 435/29 “Identified virulence factors of UPEC: Iron Uptake.” http://www. (58) Field of Classification Search .................. 424/9.1, mgc.ac.cn/cgi-bin/VFS/vfs.cgi?Genus-Escherichia 424/9.2, 234.1, 241.1; 435/4, 8, 29 &Species=UPEC&Keyword=Iron%20uptake (accessed on Jan. 12, See application file for complete search history. 2006). (56) References Cited (Continued) U.S. PATENT DOCUMENTS Primary Examiner Rodney P. Swartz (74) Attorney, Agent, or Firm K&L Gates LLP; Stephanie 4,442,203 A 4, 1984 Varshavsky .................... 435/6 Seidman 4,722,848 A 2f1988 Paoletti et al. .. ... 424,199.1 4,778,759 A 10/1988 Szalay et al. ................ 435/477 4,870,172 A 9, 1989 Okami et al. ............... 540,460 (57) ABSTRACT 4,912,199 A 3/1990 Lown et al. ................. 530,331 5,221,623 A 6/1993 Legocki et al. ...... ... 435/252.3 5,300,436 A 4, 1994 Goldstein et al. ........... 435/190 Methods for detecting a microorganism or cell in a subject 5,550,050 A 8, 1996 Holland et al. .............. 435/.382 and methods for detecting, imaging or diagnosing a site, 5,554,507 A 9, 1996 Grossman et al. ............. 435/32 disease, disorder or condition in a Subject using microorgan 5,639,275 A 6/1997 Baetge et al. ... ... 604,891.1 isms or cells and methods that microorganisms or cells for 5,646.298 A 7, 1997 Powell ....................... 548/427 treating a disease, disorder or condition are provided. Sites, 5,650,135 A 7/1997 Contaget al. ................ 4249.1 diseases and disorders include sites of cell proliferation, pro 5,650,148 A 7, 1997 Gage et al. ...... ... 424/93.2 liferative conditions, neoplasms, tumors, neoplastic disease, 5,653,975 A 8/1997 Baetge et al. ... ... 424/93.1 wounds and inflammation. Also provided are microorgan 5,656,481 A 8/1997 Baetge et al. ............... 435/325 5,676,943 A 10/1997 Baetge et al. ............ 424.93.21 isms and cells for use in the methods and compositions, 5,693,533 A 12/1997 Raney et al. ................ 435,366 combinations and kits, including diagnostic and pharmaceu 5,704,910 A 1/1998 Humes ....................... 604/5O2 tical compositions, containing a microorganism or cell. 5,710,137 A 1/1998 Fisher ......................... 514,44 5,800,828 A 9, 1998 Dionne et al. ............... 424/422 18 Claims, No Drawings US 7820,184 B2 Page 2 U.S. PATENT DOCUMENTS 2010, 0008946 A1 1/2010 Szalay et al. ............. 424,199.1 6,559,130 5/2003 Sukumar .................... 514,100 FOREIGN PATENT DOCUMENTS 6,589,531 T/2003 Andino-Pavlovsky et al. ....................... 424,199.1 EP O86.1093 9, 1998 6,596,279 B1 T/2003 Paoletti et al. ........... 424/199.1 EP 1 281 767 2, 2003 6,623,721 B2 9, 2003 Flanagan et al. .. ... 424/1.65 EP 1281772 A1 2, 2003 6,649,143 B1 11, 2003 Contag et al. ................ 4249.1 EP 1489 164 12, 2004 6,649,159 B2 11, 2003 Yang et al. ..... 424/93.21 EP 1512746 3, 2005 6,652,849 B2 11, 2003 Brown et al. ... ... 424/93.2 EP 1526.185 4/2005 6,685,935 B1 2, 2004 Pawelek et al. ... ... 424/93.2 JP 2002097,144 4/2002 6,743,967 B2 6, 2004 Hadlaczky et al. ............ 800/25 WO WO 88/OO617 1, 1988 6,759,038 B2 T/2004 Tan et al. .......... ... 424/93.21 WO WO96/4O238 12/1996 6,916,462 B2 7/2005 Contag et al. ................ 424, 9.6 WO WO97, 18841 5, 1997 6,939,691 B1 9, 2005 Khosla et al. ................. 435/76 WO WO 98,14605 4f1998 6,962,696 B1 11/2005 Bermudes et al. ... 424/93.4 WO WOOOf73479 12/2000 6,984,374 B2 1, 2006 Szalay et al. ................. 4249.1 WO WOO1? 12234 2, 2001 7,045,313 B1 5, 2006 Moss et al. .... ... 435/69.1 WO WOO1/14579 3, 2001 7,662,398 B2 2, 2010 Szalay et al. ...... 424,232.1 WO WOO1, 18195 3, 2001 2001/0008025 A1 T/2001 Hadlaczky et al. ............. 800, 8 WO WOO1,24637 4/2001 2001/OO29023 A1 10, 2001 Szalay et al. ................. 435.7.1 WO WOO1/25399 4/2001 2002.0054865 A1 5, 2002 Fujimorietal. .......... 424/93.21 WO WOO3,OO6069 1, 2003 2002fOO58326 A1 5, 2002 O'Sullivan et al. ......... 424/93.4 WO WOO3,O45153 6, 2003 2002.0160410 A1 10, 2002 Hadlaczky et al. ............. 435/6 WO WOO3/O57OO7 T 2003 2002fO160970 A1 10, 2002 Hadlaczky et al. ............ 514,44 WO WOO3,063593 8, 2003 2003/OOO9015 A1 1, 2003 Ulrich et al. ...... ... 536,23.1 WO WOO3,O92600 11, 2003 2003, OO31628 A1 2, 2003 Zhao et al. ................... 424, 9.6 WO WOO3,102168 12/2003 2003, OO31681 A1 2, 2003 McCartet al. ........... 424,186.1 WO WOO3,102169 12/2003 2003/OO33617 A1 2, 2003 Hadlaczky et al. ............. 800, 6 WO WO 2004/030631 4/2004 2003/0044384 A1 3, 2003 Roberts et al. .... ... 424/93.2 WO WO 2004/044 175 5, 2004 2003/00594.00 A1 3, 2003 Szalay ........... 424.93.2 WO WO 2004/069 178 8, 2004 2003/0O83293 A1 5/2003 Hadlaczky et al. ... 514/44 WO WO 2005/034949 4/2005 2003/0101480 A1 5/2003 Hadlaczky et al. .......... 800/278 WO WO 2006/048344 5, 2006 2003/O133949 Al 7/2003 Szalay et al. ............, 424/200.1 WO 2008/099001 8, 2008 2003/O161788 A1 8, 2003 Zhao et al. ................... 424, 9.6 2003. O186848 A1 10, 2003 Gorringe et al. ............... 514f6 OTHER PUBLICATIONS 2003/O198627 A1 10, 2003 Arts et al. ... ... 424/93.21 2003/0219385 A1 11, 2003 Ahrens ....... 424/9.322 “MUTAFLOR. The probiotic drug for life!”. http://www. 2003/0228261 A1 12, 2003 Szalay et al. ............... 424,934 ardeypharm.de/download.php?id=10000, (downloaded on Apr. 16. 2003/0228330 A1 12, 2003 Falkner et al. ........... 424,232.1 2008). 2004.0143861 A1 T/2004 Hadlaczky et al. ............ 800/14 “Vion and Memorial Sloan-Kettering Cancer Center Present Data on 2004/O186087 A1 9, 2004 Grafe et al. ................. 154,185 Tapet's Potential to Enhance Imaging of Solid Tumors' Press 20040191233 A1 9, 2004 O'Sullivan ................. 424,934 Release, Vion Pharmaceuticals, Inc.(2000). 2004/0213741 A1 10, 2004 Szalay et al. ... ... 424/9.6 (Bernstein, “Mechanisms of Therapeutic Activity for Gallium” 2004/O234455 A1 11, 2004 Szalay et al. ................. 424, 9.6 Pharmacol. Rev. 50(4): 665-682 (1998). 2005/0O25745 A1 2, 2005 Fujimori .................... 424.93.2 Abdul-Tehrani et al., “Ferritin Mutants of Escherichia coli Are Iron 2005, OO31643 A1 2, 2005 Szalay et al. ... 424,199.1 Deficient and Growth Impaired, and fur Mutants are Iron Deficient” 2005, OO63993 A1 3, 2005 Schlom et al. ........... 424,199.1 J. Bacteriol. 181(5): 1415-1428 (1999). 2005/0064447 A1 3, 2005 Huang et al.
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