USOO782O184B2 (12) United States Patent (10) Patent No.: US 7820,184 B2 Stritzker et al. (45) Date of Patent: Oct. 26, 2010 (54) METHODS AND COMPOSITIONS FOR 5,833,975 A 1 1/1998 Paoletti et al. ............. 424.93.2 DETECTION OF MICROORGANISMS AND 5,976,796. A 1 1/1999 Szalay et al. ................... 435/6 SirNRTREATMENT OF DISEASES AND 6,025,155 A 2/2000 Hadlaczky et al. ......... 435/69.1 6,045,802 A 4/2000 Schlom et al. ........... 424,199.1 (75) Inventors: Jochen Harald Stritzker, Kissing (DE); 6,077,697 A 6/2000 Hadlaczky et al. 435/1723 Phil Hill, West Bridgford (GB); Aladar 6,080,849 A 6/2000 Bermudes et al. .......... 536,23.7 A. Szalay, Highland, CA (US); Yong A. 6,093,700 A 7/2000 Mastrangelo et al. ......... 514,44 Yu, San Diego, CA (US) 6,099,848. 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No.: 12/008,659 6,416,754 B1 7/2002 Brown et al. ...... ... 424/93.21 6,447,784 B1 9, 2002 Bermudes et al. ........ 424/235.1 (22) Filed: Jan. 11, 2008 6,491.905 B1 12/2002 Sorscher et al. ............. 435/325 (65) Prior Publication Data 6,503,703 B1 1/2003 Palese et al. ................... 435/5 f 1 6,511,967 B1 1/2003 Weissleder et al. ............ 514,44 US 2009/O180987 A1 Jul. 16, 2009 6,548,068 B1 4/2003 Schlom et al. ........... 424,199.1 Related U.S. Application Data (63) Continuation of application No. 1 1/827,518, filed on Jul. 11, 2007. (Continued) (60) Provisional application No. 60/852,389, filed on Oct. FOREIGN PATENT DOCUMENTS 16, 2006, provisional application No. 60/830,422, filed on Jul. 11, 2006. DE 102 O9958 11, 2006 (51) Int. Cl. A6 IK 39/08 (2006.01) A6 IK 49/00 (2006.01) (Continued) CI2O I/00 (2006.01) OTHER PUBLICATIONS (52) U.S. Cl. ...................... 424/241.1; 424/9.1; 424/9.2: 424/234.1; 435/4; 435/8; 435/29 “Identified virulence factors of UPEC: Iron Uptake.” http://www. (58) Field of Classification Search .................. 424/9.1, mgc.ac.cn/cgi-bin/VFS/vfs.cgi?Genus-Escherichia 424/9.2, 234.1, 241.1; 435/4, 8, 29 &Species=UPEC&Keyword=Iron%20uptake (accessed on Jan. 12, See application file for complete search history. 2006). (56) References Cited (Continued) U.S. PATENT DOCUMENTS Primary Examiner Rodney P. 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