PCDH1 Antibody Cat. No.: 56-180

PCDH1 Antibody

Confocal immunofluorescent analysis of PCDH1 Antibody with U-251MG cell followed by Alexa Fluor 488- Western blot analysis in MDA-MB453,ZR-75-1 cell line lysates (35ug/lane). conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

This PCDH1 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 179-206 amino acids from the N-terminal region of human PCDH1.

TESTED APPLICATIONS: IF, IHC-P, WB

October 1, 2021 1 https://www.prosci-inc.com/pcdh1-antibody-56-180.html For IHC-P starting dilution is: 1:10~50

APPLICATIONS: For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

PREDICTED MOLECULAR 115 kDa WEIGHT:

Properties

This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification.

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: PCDH1

ALTERNATE NAMES: Protocadherin-1, Cadherin-like protein 1, Protocadherin-42, PC42, PCDH1

ACCESSION NO.: Q08174

PROTEIN GI NO.: 215273864

GENE ID: 5097

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein BACKGROUND: includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene.

October 1, 2021 2 https://www.prosci-inc.com/pcdh1-antibody-56-180.html REFERENCES: 1) Koppelman, G.H., et al. Am. J. Respir. Crit. Care Med. 180(10):929-935(2009)

2) Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)

3) Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004)

4) Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004)

ANTIBODIES FOR RESEARCH USE ONLY.

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October 1, 2021 3 https://www.prosci-inc.com/pcdh1-antibody-56-180.html