NEMATODE TAXONOMY and SYSTEMATICS NEM 6102 Course
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NEMATODE TAXONOMY AND SYSTEMATICS NEM 6102 Course Format: 1 hour lecture, 2 hour lab Credit Hours: 2 credits Prerequisites: None Instructor: Tesfamariam Mengistu (Tesfa), email: [email protected] Course Description: NEM 6102 will provide an introduction to history of nematode taxonomy, recent nematode taxonomy and systematics methods Learn classical and modern identification techniques Expected Learning Outcomes Upon completion of this course, student will be able to: Possess theoretical and practical background in nematode taxonomy and systematics Acquainted with classical and modern methods in nematode taxonomy Appreciate the importance of nematode systematics Recognize the value of nematode systematics Comprehends the role of classical and modern methods in nematode systematics Reference Books (available to borrow from my lab): The Biology of Nematodes, Donald Lee, 2002 Dorylaimida: free-living, predaceous and plant-parasitic nematodes. Jairajpuri and Ahmad, 1992 A taxonomic review of the suborder Rhabditida. Andrassy, 1983 Peer reviewed taxonomy articles (provided by the instructor) Evaluation Non-period evaluation (10%): Evaluation of the guided lab sessions and independent practical task Period evaluation (90%): on all elements of the theoretical part of the course by a written (80%) Grading scale A 90 - 100 points B+ 88 - 89 points B 80 - 87 points C+ 78 - 79 points C 70 - 77 points D 60 - 69 points E <60 Exams (Mid and final): All exams will be comprehensive but will emphasize material covered during lectures. All exams will be written and will include short answer and essay questions. Laboratory Exercises: Lab reports and permanent slides of at least 10 different genera of nematodes Project: New species description (using morphology and molecular methods); taxonomic paper review and critic. Lecture schedule Class 1 General taxonomy and systematics Class 2 Higher nematode taxonomy and systematics ( nomenclature) Class 3 How to write a taxonomy paper Class 4 Order Rhabditida Class 5 Order Triplonchida Class 6 Order Dorylaimida Class 7 Order Mononchida Class 8 Midterm exam Class 9 Identification guides for the most common genera of plant parasitic nematodes Class 10 Identification guides for the most common species of plant parasitic nematodes Class 11 Insect parasitic nematodes: Order Mermithida, Oxyurida Class 12 Molecular phylogeny and systematics I Class 13 Molecular phylogeny and systematics II Class 14 Final exam Lab Schedule Lab 1 Introduction Lab 2 Identification exercise: higher level classifications Lab 3 Data mining in taxonomic paper writing Lab 4 Sample processing: fixation, mounting, permanent slides Lab 5 Order Rhabditida lab exercise I Lab 6 Order Rhabditida lab exercise II Lab 7 Order Triplonchida lab exercise Lab 8 Mid-term exam Lab 9 Order Dorylaimida lab exercise Lab 10 Order Mononchida lab exercise Lab 11 Identification of entomopathogenic nematodes Lab 12 Molecular methods (DNA extraction, PCR, cloning) Lab 13 Molecular methods (Sequencing, phylogeny and common bioinformatics methods) Lab 14 Final exam .