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УТИЦАЈ УСЛОВА МАСОВНОГ УЗГОЈА НА СЕЛЕКЦИЈУ ДУЖИНЕ КРИЛА КОМАРАЦА ВРСТЕ Stegomyia Albopicta (Diptera: Culicidae) И ЕФЕКТИ МЕТОДЕ ОТПУШТАЊА СТЕРИЛНИХ МУЖЈАКА

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УТИЦАЈ УСЛОВА МАСОВНОГ УЗГОЈА НА СЕЛЕКЦИЈУ ДУЖИНЕ КРИЛА КОМАРАЦА ВРСТЕ Stegomyia Albopicta (Diptera: Culicidae) И ЕФЕКТИ МЕТОДЕ ОТПУШТАЊА СТЕРИЛНИХ МУЖЈАКА УНИВЕРЗИТЕТ У НОВОМ САДУ ПОЉОПРИВРЕДНИ ФАКУЛТЕТ Департман за Фитомедицину и заштиту животне средине УТИЦАЈ УСЛОВА МАСОВНОГ УЗГОЈА НА СЕЛЕКЦИЈУ ДУЖИНЕ КРИЛА КОМАРАЦА ВРСТЕ Stegomyia albopicta (Diptera: Culicidae) И ЕФЕКТИ МЕТОДЕ ОТПУШТАЊА СТЕРИЛНИХ МУЖЈАКА ДОКТОРСКА ДИСЕРТАЦИЈА Ментори: Кандидат: Проф. др Душан Петрић M.Sc. Дубравка Пудар Проф. др Romeo Bellini Нови Сад, 2018. УНИВЕРЗИТЕТ У НОВОМ САДУ ПОЉОПРИВРЕДНИ ФАКУЛТЕТ КЉУЧНА ДОКУМЕНТАЦИЈСКА ИНФОРМАЦИЈА Редни број: РБР Идентификациони број: ИБР Тип документације: Монографска документација ТД Тип записа: Текстуални штампани материјал ТЗ Врста рада (дипл., маг., докт.): Докторска дисертација ВР Име и презиме аутора: M.Sc. Дубравка Пудар АУ Ментор (титула, име, презиме, др Душан Петрић, редовни професор звање): др Romeo Bellini, редовни професор МН Наслов рада: Утицај услова масовног узгоја на селекцију дужине НР крила комараца врсте Stegomyia albopicta (Diptera: Culicidae) и ефекти методе отпуштања стерилних мужјака Језик публикације: српски ЈП Језик извода: срп. / енг. ЈИ Земља публиковања: Република Србија ЗП Уже географско подручје: АП Војводина УГП Година: 2018 ГО Издавач: ауторски репринт ИЗ Место и адреса: 21000 Нови Сад, Трг Доситеја Обрадовића 8 МА Физички опис рада: 8 поглавља / 207 страница / 138 слика / 9 графикона / ФО 20 табела / 323 референцe / биографија и захвалница Научна област: Биотехничке науке НО Научна дисциплина: Ентомологија НД Предметна одредница, кључне Техника стерилизације инсеката, Aedes albopictus речи: [Stegomyia albopicta] ПО УДК 597.771:533.693(043.3) Чува се: Библиотека Пољопривредног факултета, ЧУ Универзитета у Новом Саду Важна напомена: нема ВН Извод: ИЗ Aedes albopictus [Stegomyia albopicta] (Skuse 1895) (Diptera: Culicidae) је тренутно најекспанзивнија инванзивна врста комараца на свету. Ова врста је од изузетног медицинског значаја, пошто су женке вектори преко 20 арбовируса (проузроковача денге, чикунгуња грознице, различитих енцефалитиса и др.) и нематода рода Dirofilaria. Интрoдуковање врсте Ae. albopictus у Србију је први пут забележено 2009. год. и још увек је локализована на граничним прелазима са Хрватском и Црном Гором. Услед изложености опасности од продора ове врсте у Србију и могуће интродукције болести чији се проузроковачи преносе овим вектором, наша земља се укључује у истраживања о техници стерилизације инсеката (Sterile Insect Technique - SIT), са намером да се SIT интегрише у стратегије које могу спречити насељавање Ae. albopictus. Истраживања у циљу побољшања продуктивности масовног узгоја, као и квалитeта масовно узгојених мужјака, су од фундаменталног значаја за успех SIT-а. Ова студија је, у свом првом делу, била фокусирана на поређење утицаја три различите величине кавеза за масовни узгој Ae. albopictus, на дужину крила, преживљавање адулта и продукцију јаја, током 20 генерација колонизације; и одређивање оптималне величине кавеза за стандардни масовни узгој. Дужина крила је одабрана као значајна морфолошка промењива, која је повезана са виталношћу гајених комараца кроз општу величину тела. За мерење дужине крила (по линеарном методу), као и за бројање јаја, коришћен је компјутерски програм за обраду и анализу слике (ImageJ). Колонија Ae. albopictus коришћена у овом експерименту, потекла је од jaja сакупљених у природи и одржавана је у комори са контролисаним климатским условима у лабораторији Centro Agricoltura Ambiente (САА) „Giogio Nicoli“, Crevalcore, Италија. У кавезе од плексигласа 40 x 40 x 40 cm (C1), 100 x 20 x 100 cm (C2) и 100 x 65 x 100 cm (C3) интродукована је једнака густина адулта (20 адулта/литри), док је однос полова био 1:1. Током целог огледа је укупно процесуирано око 365.600,00 комараца. Сви тестирани параметри испољили су сличну динамику опадања својих вредности приближно до средине колонизационог периода, да би затим уследио пораст. Опоравак популације комараца у другом постпериоду је био много израженији у кавезима С1 и С2, него у кавезу С3. Узгој комараца у кавезу С1 испољио је најнижи негативан утицај на оригиналне каркатеристике популације комараца. Пре примене SIT-a у циљу сузбијања природне популације комараца, неопходно је оцентити квалитет масовно узгојених инсеката, компетитивност лабораторијски узгојених стерилисаних мужјака у односу на фертилне мужјаке из дивље популације (у процесу парења са женкама), као и њихову способност да индукују стерилитет у циљнoj популацији. У складу са тим, други део ове студије је био фокусиран на оцену релавантних параметара у полу-природним условима. У ове сврхе коришћена су 4 велика кавеза (тзв. тунела) димензија 8 x 5 x 2,8 m, постављена у вегетацију у приградској области Болоње (CAA), како би се што боље симулирали природни услови. Однос стерилисаних мужјака: фертилих мужјака: неспарених женки (virgo intacta) био је константан (100:100:100), а примењена доза ирадијације 30 Gy. Коришћена су три различита соја Ae. albopictus: два лабораторијска и један дивљи. У третману су испитиване 4 различите комбинације сојева (у укупно 15 понављања) базиране на два испитивана соја; док је у контроли (100 фертилних мужјака:100 неспарених женки) коришћен само један лабораторијски сој (у 4 понављања). Током трајања целог експеримента укупно је за интродукцију у тунеле издвојено 5300 Ae. albopictus (3400 мужјака и 1900 женки). Резидуални фертилитет мужјака (у просеку: 2,82 % на 30 Gy ), као и уочене вредности CIS и Fried индекса (у просеку свакa око 1), били су веома задовољавајући, указујући да су стерилни и фертилни мужјаци били приближно једнако компетитивни, и да је доза ирадијације од 30 Gy била веома добар избор за стерилисање мужјака (оптимизација баланса између виталности мужјака и нивоа стерилитета). Додатно је праћено још неколико важних индикатора квалитета испитиваних сојева: почетни морталитет мужјака, дневна стопа преживљавања, реаговање на утицај метеоролошких фактора, и проценат женки преосталих после раздвајања полова. Сви испитивани сојеви су се одликовали добрим вредностима одабраних индикатора квалитета. Датум прихватања теме од стране 05.03.2015. Сената: ДП Датум одбране: ДО Чланови комисије: (име и презиме / титула / звање / назив организације / статус) КО _______________________________________________ др Душан Петрић, редовни професор за ужу научну област Ентомологија, Пољопривредни факултет, Нови Сад, Департман за Фитомедицину и заштиту животне средине; ментор _______________________________________________ др Romeo Bellini, редовни професор за ужу научну област Ентомологија, Department of Medical and Veterinary Entomology, Centro Agricoltura Ambiente “G. Nicoli” (CAA), IAEA Collaborating Centre SUSTENIA S.r.l. Crevalcore, Italy; ментор _______________________________________________ др Марија Згомба, редовни професор за ужу научну област Фитофармација, Пољопривредни факултет, Нови Сад, Департман за Фитомедицину и заштиту животне средине; председник UNIVERSITY OF NOVI SAD FACULTY OF AGRICULTURE KEY WORDS DOCUMENTATION Accession number: ANO Identification number: INO Document type: Monograph documentation DT Type of record: Textual printed material TR Contents code: PhD thesis CC Author: Dubravka Pudar, M.Sc. AU Mentor: Dušan Petrić, PhD, full professor MN Romeo Bellini PhD, full professor Title: Effect of mass production on the wing size selection of Stegomyia TI albopicta (Diptera: Culicidae) and evaluation of sterile male release efficiency Language of text: Serbian LT Language of abstract: English / Serbian LA Country of publication: The Republic of Serbia CP Locality of publication: The Province of Vojvodina LP Publication year: 2018 PY Publisher: Author’s reprint PU Publication place: 21000 Novi Sad, Sq. Dositej Obradović 8 PP Physical description: 8 chapters / 207 pages / 138 images / 9 graphs / 20 tables / 323 PD references / biography and acknowledgment Scientific field Biotechnical sciences SF Scientific discipline Entomology SD Subject, Key words Sterile Insect Technique, Aedes albopictus [Stegomyia albopicta] SKW UDC 597.771:533.693(043.3) Holding data: Library of the Faculty of Agriculture, University of Novi Sad HD Note: None N Abstract: AB Currently, the most expansive invasive mosquito species in the world is Aedes albopictus [Stegomyia albopicta] (Skuse 1895) (Diptera: Culicidae). This species is of paramount medical importance, as females are efficient vectors of more than 20 viral pathogens (including dengue, chikungunya, different encephalitis viruses etc.) as well as nematodes of genus Dirofilaria. Introduction of Ae. albopictus in Serbia has been reported for the first time in 2009, and it is still restricted to the border crossings with Croatia and Montenegro. Due to the elevated risk of Ae. albopictus spreading and establishing across Serbia and possible introduction of vector borne diseases, our country is getting involved in the research of Sterile Insect Technique (SIT), with intention to integrate SIT into strategies that can prevent Ae. albopictus detrimental vectorial consequences in our region. Research to improve the productivity of mass-rearing, as well as the quality of mass reared males is of essential importance for the success of SIT strategy. This study, in its first part, compared the influence of three differently sized cages for Ae. albopictus mass rearing on wing length, adult survival and egg production during 20 generations of reared mosquitoes, and determined the optimal cage size for standard mass rearing. The wing length was selected as an easy to measure morphological variable well correlated with fitness of reared mosquitoes. An open source image processing and analysis programme (ImageJ)
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