HikobiaHikobial4：１４３－１４７．２００４ 14: 143-147.2004

PhylogeneticPhylO窪eneticandmorphOlO=icalmtesⅢＩノルＣＤ〃"剛〃昭肌oiKiguchi and morphological notes on Uleobryum naganoi Kiguchi eteraL(,Musci)’ ale (Pottiaceae, Musci) 1

HIROYUKIHIRoYuKISATQHⅡRoMITsuBoTA,ToMIoYAMAGucHIANDHIRoNoRIDEGucH１ SATO, HIROMI TSUBOTA, TOMIO YAMAGUCHI AND HIRONORI DEGUCHI

SATO,SATO,Ｈ､,TsuBoTA,Ｈ､,YAMAGucHI,Ｔ､＆DEGucHI,H2004Phylogeneticandmor- H., TSUBOTA, H., YAMAGUCHI, T. & DEGUCHI, H. 2004. Phylogenetic and mor­ phologicalphologicalnotesonU/eo6Mｲﾉ'z〃αgα"ojKiguchietα/､(Pottiaceae,Musci）Hikobia notes on Uleobryum naganoi Kiguchi et al. (Pottiaceae, Musci). Hikobia 14:l4：１４３－１４７． 143-147.

UleobryumＵ/eo6/Ｗｍ〃αgα"ojKiguchiejα/､,endemictoJapanwithalimitednumberofknown naganoi Kiguchi et aI., endemic to Japan with a limited number of known locations,locations,isnewlyreportedffomShikoku,westernJapanThroughcarefUlexamina- is newly reported from Shikoku, western Japan. Through careful examina­ tionoffTeshmaterial,rhizoidalmberfbnnationisconfinnedfbrthefirsttime・The ， tion of fresh material, rhizoidal tuber formation is confirmed for the first time. The phylogeneticpositionofthiscleistocalpousmossisalsoassesseｄｏｎｔｈｅｂａｓｉｓｏｆｍaxi-phylogenetic position of this cleistocarpous is also assessed on the basis of maxi­ mummumlikelihoodanalysisof′bcLgenesequences、ThecuITentpositioninthePot- likelihood analysis of rbcL gene sequences. The current position in the Pot­ tiaceaetiaceaeissUpportedandacloserelationshiptoEpheme'ｗｍｓｌｐｊ""/ＯＳ"川ssuggested is supported and a close relationship to Ephemerum spinulosum is suggested.

Ｈ１'わⅦkjStJro,Ｈ７ｒｏ〃ＺＭ６ｏｔａ，Tb〃ｏＹＺＪ〃αg"ｃｈｊ＆Ｈｉ７ｏ"orjDeg"cAj,Ｄｅｐ”""e"'Hiroyuki Sato, Hiromi Tsubota, Tomio Yamaguchi & Hironori Deguchi, Department of０/Ｂｊｏ/ogicα/Ｓｂｊｅ"Ce,ｏｒα`"α/eSbAoo/ｑ/肱e"Ｃｅ,肋Cs〃〃α〔ﾉﾉ､ﾉe'Ｍリノ,ＫｔＪｇα〃‐ Biological Science, Graduate School of Science, Hiroshima University, Kagami­ ｙａｍαﾉｰ3-/,Higashj-/iかCsﾉi〃α-s/ij,価'０s/i伽α７３４８５２．ﾉﾋﾘpα〃yama 1-3-/, Higashi-hiroshima-shi, Hiroshima 739-8526, Japan.

arearemissingMolecularphylogeneticsmdieshave mlssmg. Molecular phylogenetic studies have Introduction beenmadetoclarifythephylogeneticpositionofbeen made to clarify the phylogenetic position of Thegenusueo6'Ｗ腕isasmallgenuscom-The genus Uleobryum is a small genus com­ somesomemosseslackingperistomes(Goffinet＆Cox lacking peristomes (Goffinet & Cox prisingonlythreespecies(Kiguchiejα/,1996；prising only three species (Kiguchi et al. 1996; 2000;Goffinete/α/､1998,2002;Heddersonem/、2000; Goffinet et al. 1998, 2002; Hedderson et al. Zanderl993,1994;Zander＆Dixonl999LwithZander 1993, 1994; Zander & Dixon 1999), with 2004;Stechl999)．Theseresearchesdemonstrate2004; Stech 1999). These researches demonstrate adistributionranｇｅｉｎｔｈｅＷｅｓｔｈｄｉｅｓ，Mexicqa distribution range in the West Indies, , thatmoleculardataprovideuswithinfb､nationthat molecular data provide us with infonnation Peru,Brazil,Australia,andJapanThegenusisPeru, Brazil, Australia, and Japan. The genus is usefUltobetterunderstandsuchenigmaticcleisto-useful to better understand such enigmatic cleisto­ characterizeｄｂｙｉｔｓｓｍａｌｌｓｉｚｅ，acostawithtwocharacterized by its small size, a costa with two carpousmosses・hthispaper，rhizoidaltubercarpous mosses. In this paper, rhizoidal tuber stereidbandsandelongateadaxialepidennalstereid bands and elongate adaxial epidennal fbnnationfbrU/eo6MJm〃αgα"ｏｊｉｓｎｅｗｌｙｒｅ－fonnation for Uleobryum naganoi is newly re­ cells，spheroidalcleistocalpouscapsuleswithacells, spheroidal cleistocarpous capsules with a ｐｏｒｔｅｄａｎｄｔｈｅｐｈｙｌogeneticpositionofthisspe-ported and the phylogenetic position of this spe­ hyalineseta，transparentcapsulewallsofwhichhyaline seta, transparent capsule walls of which cieｓｉｓａｌｓｏｄｉｓｃｕｓｓｅｄｏｎｔｈｅbasisofribulosecies is also discussed on the basis of ribulose cellsaremamillose，withslightlythickenedfreecells are mamillose, with slightly thickened free 1,5-bisphosphateL5-bisphosphatecarboxylase/oxygenaselarge carboxylase/oxygenase large walls，andconic-campanulatecalyptraethatarewalls, and conic-campanulate calyptrae that are subunit（'6cL）genesequenceswithmaximumsubunit (rbcL) gene sequences with maximum roughenedapically(Zanderl993)．Ithasbeenroughened apically (Zander 1993). It has been likelihoodanalysis,togetherwithareportofnewlikelihood analysis, together with a report of new placedinthePottiaceaesinceitsestablishmentasplaced in the Pottiaceae since its establishment as habitatinJapan．habitat in Japan. anindependentgenusbyBrotherus(1906)．meo-an independent genus by Brotherus (1906). Uleo­ 6M/ｍ〃αgzJ"ojKiguchierα/､hasbeendescribedbryum naganoi Kiguchi et al. has been described MaterialsandmethodsMaterials and methods asendemictoJapanbyKiguchiem/､(1996),andas endemic to Japan by Kiguchi et al. (1996), and ischaracterizedby（１）linear-1anceolateleaves，is characterized by (1) linear-lanceolate leaves, TotalDNAwasextractedffom廿eshmaterialTotal DNA was extracted from fresh material (2)stomataonthemiddleoftheupperhalfofthe(2) stomata on the middle of the upper half of the usingthemodificationsofaphenol-chlorofbImusing the modifications of a phenol-chlorofonn capsule,ａｎｄ(3)unlobedcalyptrae，Thesystem-capsule, and (3) unlobed calyptrae. The system­ method（TsubotaeM/、1999)．PCRamplifica-method (Tsubota et al. 1999). PCR amplifica­ aticpositionofcleistocalpousmossesisequivo-atic position of cleistocarpous mosses is equivo­ tionsanddirectsequenceanalyseswerecarriedtions and direct sequence analyses were carried calwhenconclusionsarelimitedtomolphologi-cal when conclusions are limited to morphologi­ outusingstandardtechniquesDetailsandfUr-out using standard techniques. Details and fur­ calcharacters,particularlywhenmajorcharacterscal characters, particularly when major characters therinfbnnationfbrDNAextractingprotocols，ther infonnation for DNA extracting protocols, amplificationprimers，PCRprotocolsandthoseamplification primers, PCR protocols and those lContributionffomtheLaboratoryofPlantTaxonomyI Contribution from the Laboratory of ofsequencingreactionsaredescribedinTsuboｔａof sequencing reactions are described in Tsubota andEcology,HiroshimaUniversity.Ｎ・Ser､No.５３８．and Ecology, Hiroshima University. N. Ser. No. 538. ｅｍ/､(1999,2000,2002)．et al. (1999, 2000, 2002). 1４４144 ＨｉｋｏｂｉａＶｏＬｌ４,No.２，２００４Hikobia Vol. 14, No.2, 2004

FiftysixmossspecieswereincludedintheFifty six moss species were included in the haplolepideousmossesincludingsomediplolepi-haplolepideous mosses including some diplolepi­ analysesandthesequencesweremanuallyanalyses and the sequences were manually deousmosses・Fortheanalyses,atotalofL210deous mosses. For the analyses, a total of 1,210 alignedOutgrouptaxawereselectedbasedonaligned. Outgroup taxa were selected based on sites(84.73％ｉｎ1,428bpoftheentiresequence）sites (84.73% in 1,428 bp of the entire sequence) thephylogenetichypothesisproposedbythe phylogenetic hypothesis proposed by of5676cLsequenceswereusedof 56 rbcL sequences were used. Magombo(2003)andTsubotaejaL(2003)．Magombo (2003) and Tsubota et at. (2003). lnfbnnationonthespecimensfTomwhichInfonnation on the specimens from which AphylogenetictreewasconstructedusingA phylogenetic tree was constructed using DNAwasextractedwiththeaccessionnumbersDNA was extracted with the accession numbers maximumlikelihoodcriteriａ（ＭＬ；Felsensteinmaximum likelihood criteria (ML; Felsenstein isgivenbelow：is given below: l981）AnNJtreewasobtainedusingNjdistin1981). An NJ tree was obtained using Njdist in Ｂﾉﾋﾞ)′o抑ﾉｶﾉ"ｍ〃o'WgJc""ｚｓｕｂｓｐノ叩o"Jc""’Bryoxiphium Ilorvegicum subsp. japonicum ’ MOLPHY23b3（Adachi＆Hasegawal996）MOLPHY 2.3b3 (Adachi & Hasegawa 1996) (Berggr.)Ａ・L6ve＆DL6ve,AB194720,Japan，(Berggr.) A.Love & D.Love, ABI94720, Japan, withHKY85model(Hasegawaetα/､1985),andwith HKY85 model (Hasegawa et at. 1985), and Honshu，Tottori-ken，Hino-gun，KoufU-chqMt・Honshu, Tottori-ken, Hino-gun, Koufu-cho, Mt. thebestMLtreewassearchedusi､ｇＮｕｃＭＬｉｎthe best ML tree was searched using NucML in Karasugasen，1,200ｍａｌｔ.,Ｊｕｎｅ18,2000,ＬｅｇＳ・Karasugasen, 1,200 m alt., June 18, 2000, Leg. S. ＭＯＬＰＨＹｗｉｔｈｔｈｅＮＪｔｒｅｅａｓａstarttopologyfbrMOLPHY with the NJ tree as a start topology for NogawainhbHDeguchi36501(HIRO)．Nogawa in hb. H. Deguchi 36501 (HIRO). alocalrearrangementalgorithma local rearrangement algorithm. UleobryumＵ/eo6ﾉＷ"z〃αgzJ"ojKiguchie/αL,ＡＢ１９４７１７， Ilaganoi Kiguchi et al., AB 194717, New76cLsequencesweredetenninedfbrfburNew rbcL sequences were detennined for four Japan,Shikoku,Kagawa-ken,Ayauta-guLAyakami-Japan, Shikoku, Kagawa-ken, Ayauta-gun, Ayakami­ ● species:＊Br〕ﾉoxjphj""z〃orvegzczJ"１subsp,ﾉ叩o"ノーspecies: *Bryoxiphium norvegicum subsp. japoni­ cho,NagaraLake,Ｃａ」ＯＯｍａｌｔ.[associatedwithcho, Nagara Lake, ca. 100 malt. [associated with c"腕(ABl94720),＊meo6'Ｗｍ"αgzJ"oj(AB1947cum (AB 194720), * Uleobryum naganoi (AB 1947 印/jemelw"Tspj""/Cs""７Bruch＆Schimp],Dec・Ephemerum spinulosum Bruch & Schimp.], Dec. 17),肌maco""ove耐α(ABl94718)and*Ｅｌｐｈｅ－17), Weissia controversa (AB 194718) and *Ephe­ 13,2003,'3,2003,HSato377(HIRO)． H. Sato 377 (HIRO). "'e'wmgpj"[ｲﾉCs"〃（AB194719)．Threespeciesmerum spinulosum (ABI94719). Three species Weissiaﾘﾘﾉi2jlssjaco"〃ove'ＦａＨｅｄｗ.,ＡＢ１９４７１８,Japan， controversa Hedw., AB194718, Japan, withanasteriskwereshownasanentiresequencewith an asterisk were shown as an entire sequence Honshu,Hiroshima-ken,Higashi-hiroshima-shLHonshu, Hiroshima-ken, Higashi-hiroshima-shi, 1,428l,428bplongAdatamatrixwaspreparedfbr bp long. A data matrix was prepared for ca200ma1t.,July3,2003,ＨＳａｔｏｌ６１(HIRO）ca. 200 m alt., July 3,2003, H. Sato 161 (HIRO). analysesanalysesofphylogeneticrelationｓｈｉｐｓｏｆｔｌｌｅ of phylogenetic relationships of the Ｅﾄﾞﾌﾊeme'w"ｚＳｐｊ""/Cs""ZBruch＆Ｓｃｈｉｍｐ，Ephemerum spinulosum Bruch & Schimp.,

Ｌ A iill

500~mⅡ、 、

L魁

、の●◆ ~m４０Ⅱ、

Fig.Fig・LRhizoidaltubersofU/eo6ひ'z"〃〃αgα'MKiguchieZα/、 1. Rhizoidal tubers of UteoblJ'ulI1 nagalloi Kiguchi et at. A.APlantwithacapsuleandrhizoids・B-FRhizoidalmbers(BcorrespondingtobinA;Ｃｔｏｃ)． Plant with a capsule and rhizoids. B-F. Rhizoidal tubers (B corresponding to b in A; C to c). PhotographedPhotographedanddrawnfiPomHSato３７７． and drawn from H. Sato 377. H.Ｈ・ＳＡＴＯ,Ｈ・ＴｓｕＢｏＴＡ,ＴＹＡＭＡＧｕｃＨＩＡＮＤＨ、ＤＥＧｕｃＨＩ SATO, H. TSUBOTA, T. YAMAGUCHI AND H. DEGUCHI 1451４５

ABＡＢｌ９４７１９，Japan，Shikoku，Kagawa-keL 194719 , Japan, Shikoku, Kagawa-ken, bersｂｅｒｓｏｆＯＴＵｓｗｅｒｅｓｈｏｗｎｉｎｐａｒｅｎｔｈｅｓｅｓｉｎＦｉｇ． of OTUs were shown in parentheses In Fig. Ayauta-gun,Ayauta-gun，Ayakami-cho,NagaraLake，cal00 Ayakami-cho, Nagara Lake, ca. 100 2.2． malt.,malt.,Decl3,2003,ＨＳａｔｏ３７８(HIRO)． Dec. 13,2003, H. Sato 378 (HIRO). TheTheotherflftytwosequenceswereobtained other fifty two sequences were obtained fromfTomtheDNAdatabases,andtheaccessionnum- the DNA databases, and the accession num-

一一議 Hypnum plumaeforme (AB029384) n------Sematophyllum subhumile (AB039675) Entodon challengeri (AB050993) .---- Plagiothecium nemorale (AB029387) -11-______Adelothecium bogotense (ABI03354) 100 Funaria apophysata (AF0055 14) Physcomitrella patens (X74156) 色 Enc.lypta streptocarpa (AF478239) Encalypta rhaptocarpa (A1275 167) 100 Ephemerum spinulosum (ABI94719)

寺

Uleobryum naganoi (ABI94717) の国の。⑤二」。』 Pseudosymblcpharis schimpcriana (AF226805) Weissia controvcrsa (AB 194718) Barbul. convoluta (A1'478225) 100 Pottia Intemledia (ABI25592) ● Tortula obtusissima (A1'226823) muticum (A1'231 078) 55 Streptopogon calymperes (A1'47823I ) Tortula ruralis (TRU275 169) Aulacopilum hodgkinsoniae (AF005545) Venturiell. sinensis (AF005546) "------Uleastrum palmicola (AF005547) Kiaeria blyttii (AF231306) Rhabdoweisia crenulata (AF005544) -l.....___ Dieranoweisia cirrata (AF23 1297 ) Ditrichum pallidum (AF23 1302) 100 Pleuridium acuminatum (AF231312) Hypodontium pomiforme (AF226803)３８０ ｐＰ７１ 65 Paraleucobryum enerve (AF226827) 57５ 100 OrthodicranumＤｒｔｈｏｄ i"ulvum (AF231311) Dicranum condensatum (AI'231298) ｕｉﾆｾﾞDicnemon seriatum (AI'478229) --""""1L___ Eucamptodon muelleri (A1'231319)9） n-----~ Fissidens mooreaea (A1'2268I5８１０ 0) Ⅲ 杵Ｌ－ｊ里『'---=L Fissidens osmundoidesロ (A1'231 0(9) Fissidens dubius (AF231303) Amphidium lapponicum (AF005543) IF"'--- Timmiella crassinervis (AF478236) Li二三iili撰驚Scouleria aquatica (AF226822) Drummondia obtusifolia (AF232697) Bryoxiphium norvegicum sUbsp.japonicum (ABI94720)94720） Brothera leana (AB 122033) 81 Dicranodontium denudatum (AB 122034) Leucobryumjuniperoideum (AB 124786) Leucobryum sanctum (AB 124787) 1.-___ Campylopus argyrocaulon (AF231315) '"=--- Archidium stellatum (AF23 1(66) Blindia acuta (A1'226817) Ptychomitrium gardneri (AF005549) Grimmia laevigata (AF478230) 72 Mitthyridium fasciculatum (A1'226780) 94 Syrrhopodon tortilis (AF226782) b.____ Calymperes palisotii (A1'226791) ....------Octoblepharum albidum (AF226795) Diphyscium longifolium (AF4782 I 7) Diphyscium fulvifolium (AF478222) Ｂ■L--....I ０.Olsubstitutions/site0.0 t substitutions/site

Ｆｉｇ２、PhylogeneticpositionofU/eo6Ml"1〃αgα"ｏｊ．ThemaximumlikelihoodtreeofthealignedFig. 2. Phylogenetic position of Uleoblyum naganoi. The maximum likelihood tree of the aligned r6cLgenesequences,1,210bpof561bcLgenesequences(HKY85model[Hasegawaetα/､1985]；rbcL gene sequences, 1,210 bp of 56 rbcL gene sequences (HKY85 model [Hasegawa et at. 1985]; 2α/β＝4865;ＩｎＬ＝-9120.08±392.04ｂｙＮｕｃＭＬ);andthecladeswithinthePottiaceaeclade2 a I [3 = 4.865; In L = - 9120.08 ± 392.04 by NucML); and the clades within the Pottiaceae clade. ThenumbersinparenthesesshowaccessionnumbersofeachOTU、ThehorizontallengthofeachThe numbers in parentheses show accession numbers of each OTU. The horizontal length of each branchcorrespondstotheestimatednumberofnucleotidesubstitutions・Therootisarbitrarilybranch corresponds to the estimated number of nucleotide substitutions. The root is arbitrarily placedonthebranchleadingtoDjp伽cjzl"，ノo"g(/Ｍ""Z（AF478217）ａｎｄ、ルノＷＭ"〃placed on the branch leading to Diphyscium /ongi[olium (AF4782 17) and D. fu/v(/olium (AF478222）(AF478222). 1461４６ HikobiaHikobiaVol」４，No.２，２００４ Vol. 14, No.2, 2004

those of the genera of the family Funariaceae and Results thoseofthegeneraofthefamilyFunariaceaeand thethesystematicpositionofthegenusintheorder systematic position of the genus in the order RhizoidalRhizoidaltubersconsistofasingle,bifUrcated tubers consist of a single, bifurcated FunarialesFunarialesprovedtobedoubtfUl,judgingfrom proved to be doubtful, judging from ororbranchedcell-row，tingedreddish-brown， branched cell-row, tinged reddish-brown, thethepresentresultandtheirdissimilarmoIpho- present result and their dissimilar morpho­ whichwhichissimilarincolortotherhizoidsThetu- is similar in color to the rhizoids. The tu­ logicallogicalcharacters、HenceEpAeme'wmshouldbe characters. Hence Ephemerum should be berberceUsarebarrel-shaped，moreorlessisodia- cells are barrel-shaped, more or less isodia­ transferredtransfierredfi-omtheEphemeraceaetothePottia- from the Ephemeraceae to the Pottia­ metric,metric,ca20-551LLmthick；eachoftbemisusu- ca. 20-55 Ilm thick; each of them is usu­ ceaeceaeaswasdonebyGofHnet＆Ｂｕｃｋ(2004） as was done by Goffinet & Buck (2004). allyallyaccompaniedbyasmalllenticularinitialcelｌ accompanied by a small lenticular initial cell TheThehomologyvalue98､39％(＝Ｌ４０５ｂｐ)ｏｆ homology value 98.39% (= 1,405 bp) of ca.ｃａｌ４－３２ｌImindiameter、InitialceUsbeganto 14-32 Ilm in diameter. Initial cells began to UＵｌ〃αgα"ｏｊａｎｄＥ・ｓｐｊ""/Cs""zsuggestedmore naganoi and E. spinulosum suggested more developdevelopintoprotonemainafiewweeksafterliq- into protonema in a few weeks after liq­ detaileddetailedinvestigationisrequiredwithmorespe- investigation is required with more spe­ uiduidcultivationinapetridishatroomtemperature cultivation in a petri dish at room temperature ciesciessampledfiDmthePottiaceaetoobtainmore sampled from the Pottiaceae to obtain more (20-25"C).(20-25℃） preciseprecisephylogeneticinfbrmationonthegenera phylogenetic information on the genera TheＴｈｅＭＬｔｒｅｅｗａｓｏｂｔａｉｎｅｄｂｙＮｕｃＭＬＯｎＬ＝ ML tree was obtained by NucML (In L = UleobryumU/ｅｏ６１ＷｍａｎｄＥｌＤ/ie腕e'脚加withinthePottia- and Ephemerum within the Pottia­ -9120.08-9120.08±392.04;Fig.２)．Fourlargeclades ± 392.04; Fig. 2). Four large clades ceae.ceae・meo6Mｲmwasrelatedtoothercleisto- Uleobryum was related to other cleisto­ て correspondingcoITespondingtotheBryidae，FunariaceaQEn- to the Bryidae, Funariaceae, En­ carpouscarpousgeneraBryoce"tAoSpolaand〃αc/ｊ〕ﾉcar- genera Bryoceuthospora and Trachycar­ calyptaceae,calyptaceae,andDicranidaewereresolvedwitha and were resolved with a pj`j"脚aspreviouslysuggestedbyZander(1993,pidium as previously suggested by Zander (1993, highhighBPsupport(100％,100％,１００％,ａｎｄ９５％， BP support (100%, 100%, 100%, and 95%, 1994).1994)． respectively).respectively)．WithintheDicranidaeclade,the Within the Dicranidae clade, the Finally,Finally,populationsofU/eo6M"〃〃αgzJ"ojre- populations of Uleobryum naganoi re­ ヴ Pottiaceae-EP〃eｍｅ'w"ZcladewasresolvedwithPottiaceae-Ephemerum clade was resolved with mainmaininrestrictedlocationswhichincludeKana- in restricted locations which include Kana­ strongstrongsupport（１００％)．WithinthePottiaceae- support (100%). Within the Pottiaceae­ gawa,gawa，thetypelocality，Shizuoka，andSaitama the type locality, Shizuoka, and Saitama EphmerumE;phmelwmclade,twosubcladeswereresolved： clade, two subclades were resolved: PrefecturesPrefecmresofEastJapa、（Kiguchietα/1996； of East Japan (Kiguchi et at. 1996; thetheＵ/eo6'Wm-Elphemer""0-Ae"`/DWZ6ノビアノｉＭｓ Uleobryum - Ephemerum - Pseudosymblepharis IwatsukiIwatsuki＆Suzukil998;Kiguchi2003)．Anew & Suzuki 1998; Kiguchi 2003). A new -－肌ma-Bα'仇ﾉﾉaclade(１００％)andthePo"ｊα－Weissia-Barbula clade (100%) and the Pottia­ locationlocationisfirstreportedhereffomShikoku,west- is first reported here from Shikoku, west­ Tortula-Acaulon-Streptopogon７Ｍ"/α-化α"/o'１－s"叩rOpogo〃clade（100％)． clade (100%). ernernJapanTheplantsweregrowingonsandysoil Japan. The were growing on sandy soil WithinWithinthefionnerclade，〔ﾉﾉeo6Mﾉﾉ〃hasaclose the former clade, Uleobryum has a close mixedmixedwithgravelontheshoreofawaterreserve with gravel on the shore of a water reserve relationshiprelationshipwith助ｈｅｍｅﾉwｍ（100％）Homolo‐ with Ephemerum (100%). Homolo­ forfbrirrigation,beingassociatedwith印Aemelwm irrigation, being associated with Ephemerum gygyvaluesofthetotalr6cLgenesequｅｎｃｅｓｏｆＵ values of the total rbcL gene sequences of U spinulosum.叩j""/Cs""0． naganoi"αgα"ｏｊａｎｄＥ・叩/""/Cs"mprovedtobeidentical and E. spinulosum proved to be identical withwiththevalue98.39％(＝L405bp)． the value 98.39% (= 1,405 bp). Acknoledgements

WeWethankDrT､MatsuiandDr・TArikawafbr thank Dr. T. Matsui and Dr. T. Arikawa for Discussion constructiveconstructivesuggestionsonanearlierversionof suggestions on an earlier version of UloebryumＵ/oeZW"misacleistocarpousmoss，andthe is a cleistocarpous moss, and the thisthispaper,ａｎｄＭｒ.Ｔ，Suzukifbrcommentsand paper, and Mr. T. Suzuki for comments and gametophytesgametophytesshowfeaturescharacteristicof show features characteristic of providingprovidingecologicalinfbnnationFurtherthanks ecological information. Further thanks manymanyspeciesofthePottiaceae,ｓｕｃｈａｓ（１）obo‐ species of the Pottiaceae, such as (1) obo­ areareextendedtoDr．Ｒ・Stotlerfbrcheckingthe extended to Dr. R. Stotler for checking the vatevatetolinearlanceolateleaveswiｔｈａｎａｃｕｔｅｔｉｐ， to linear lanceolate leaves with an acute tip, EnglishEnglishtext． text. andand(2)laminacellsthataredenselypapillateon (2) lamina cells that are densely papillate on bothbothsurfacesintheupperandmiddlepartsofthe surfaces in the upper and middle parts of the LiteratureLiteraturecited cited leaves.leavesThepresentresultssuggestacloserela- The present results suggest a close rela­ tionshiptionshipofU/eo6ﾉＷ"ｚｗｉｔｈＥｌｐＡｅ"Te'w"Land of Uleoblyum with Ephemerum, and Adachi,Adachi，』．＆Hasegawa,Ml996ComputerScience J. & Hasegawa, M. 1996. Computer Science theirtheirinclusioninthePottiaceaewasstronglysup- inclusion in the Pottiaceae was strongly sup­ Monographs,Monographs，Ｚ８ＭＯＬＰＨＹｖｅｒｓｉｏ、2.3．Programs 28. MOLPHY version 2.3. Programs ported,ported,aspreviouslyreportedonthebasisofthe as previously reported on the basis of the forfbrmolecularphylogeneticsbasedonmaximum molecular phylogenetics based on maximum datadataonlps4genesequences（Gofflnet＆Cox on rps4 gene sequences (Goffinet & Cox likelihood.likelihoodl50pplnstimteofStatisticalMath- 150 pp. Institute of Statistical Math­ 2000;2000；Gofflnete／α/、２００１；Heddersone／α/、 Goffinet et at. 2001; Hedderson et al. ematics,ematics,Tokyo・ Tokyo. 2004).2004)．HeddersoneM/､（2004）ftlrtherinfierred Hedderson et at. (2004) further inferred Brotherus,Brotherus,Ｖ・Ｆ１906．Musciamazonicietsubandini V. F. 1906. Musci amazonici et subandini thatthatsomespeciｅｓｏｆ〃IgjssjainthePottiaceae some species of Weissia in the Pottiaceae U1eani.Uleani・Hedwigia45:260-288． Hedwigia45: 260-288. alsoalsoapproachEipﾉieme'ｗｍ,ｉ,e､,specieswithre- approach Ephemerum, i.e., species with re­ Felsenstein,Felsenstein，Ｊ１９８LEvolutiontreesfiPomDNAse- J. 1981. Evolution trees from DNA se­ ducedducedplantsize,immersedsporophytes,andper- plant size, immersed sporophytes, and per­ quences:quences：amaximumlikelihoodapproachJ・Ｍｏｌ a maximum likelihood approach. J. Mol. sistentsistentprotonemata・ protonemata. Evol.Ｅｖｏｌｌ７:368-376 17: 368-376. TheThecloserelationshipofEpAeme'wｍｗｉｔｈ close relationship of Ephemerum with Goffinet,GofHnet,Ｂ＆Buck,Ｗ､Ｒ・Z004Systematicsofthe B. & Buck, W. R. 2004. Systematics of the H.Ｈ・SATO,Ｈ・TsuBoTA,ＴＹＡＭＡＧｕｃＨＩＡＮＤＨ・DEGucHl SATO, H. TSUBOTA, T. YAMAGUCHI AND H. DEGUCHI 1471４７

BryophytaBryophyta(mosses):frommoleculestoarevised (mosses): from molecules to a revised weisiaceae,weisiaceaeBryopsida）NovaHedwigia68:２９１－ ). Nova Hedwigia 68: 291- classification.classiHcationlnGofHnet，Ｂ，Hollowel１，Ｗ．＆ In Goffinet, B., Hollowell, W. & 300.300． Magill,MagilLR（eds)，MolecularSystematicsof R. (eds.), Molecular Systematics of Stone,Stone,ＬＧ」984.Ｕ/eo6ﾉＷｍｃ"'７Ｍsp・nov.(Pottia- I. G. 1984. Uleobryum curtisii sp. nov. (Pottia­ Bryophytes,Bryophytes，pp205-239・MissouriBot、Garden pp. 205-239. Missouri Bot. Garden ceae)ceae）fiFomQueensland，Australia、ＪＢｒｙｏＬ１３： from Queensland, Australia. J. Bryol. 13: Press,Press,StLouis． St. Louis. 19-24.l9-24 ---－＆ＣＯＸ,Ｃ・l2000Phylogeneticrelationships& Cox, C. J. 2000. Phylogenetic relationships Tsubota,Tsubota,H､,Akiyama,H､,Yamaguchi,T&DeguchL H., Akiyama, H., Yamaguchi, T. & Deguchi, amongamongbasal-mostarthrodontousmosseswith basal-most arthrodontous mosses with H.H・Z00LMolecularphylogenyoftheSematophyl- 2001. Molecular phylogeny of the Sematophyl­ specialspecialemphasisontheevolutionarysignificance emphasis on the evolutionary significance laceaelaceae(Hypnales,Musci)basedonchloroplastr6cL (Hypnales, Musci) based on chloroplast rbcL ofoftheFunariineaeBryologistlO3:212-223. the Funariineae. Bryologist 103: 212-223. sequences.sequences、lHattoriBotLab､９０:２２１－２４０ J. Hattori Bot. Lab. 90: 221-240. ----＆Ｓｈａｗ，Ａ・’2002．Independentoriginsof& Shaw, A. J. 2002. Independent origins of ---,－，Ageno,Y､,Est6banez,Ｂ,YamaguchLT.& Ageno, Y., Estebanez, B., Yamaguchi, T. & cleistocarpycleistocarpyintheSplachnaceae：analysesof in the Splachnaceae: analyses of Deguchi,DeguchLH､2003.Molecularphylogenyofthe H. 2003. Molecular phylogeny of the cpDNAcpDNAsequencesandpolyphylyoftheVoitioideae sequences and polyphyly of the Voitioideae GrimmialesGrimmiales（Musci）basedonchloroplastr6cL (Musci) based on chloroplast rbcL (Bryophyta).(BIyophyta）SystBot27:203-208 Syst. Bot. 27: 203-208. sequences.sequences・Hikobial455-70． Hikobia 14: 55-70. ---,-,Bayer,Ｂ､』.＆Vitt,，.Ｈ、１９９８．Circumscrip- Bayer, B. J. & Vitt, D. H. 1998. Circumscrip­ ---,－，Nakao，Ｎ､，YamaguchiT.，Seki，Ｔ＆ Nakao, N., Yamaguchi. T., Seki, T. & 勺 tiontionandphylogenyoftheOrthotrichales and phylogeny of the Orthotrichales Deguchi,Deguchi，Ｈ、2000．Preliminaryphylogeneticrela- H. 2000. Preliminary phylogenetic rela­ (Bryopsida)(Bryopsida)inferredfiPomr6cLsequenceanalyｓｅｓ inferred from rbcL sequence analyses. tionshipstionshipsofthegenusBror/ie7e"αanditsallied of the genus Brotherella and its allied Am.Ａｍ・ＪＢｏｔ､８５：’３２４－１３３７． J. Bot. 85: 1324-1337. generagenera（Hypnales，Musci）basedonchloroplast (Hypnales, Musci) based on chloroplast ０ ---,，ＣＯＸ，ＣＪ.，Ｓｈａｗ，Ａ、』．＆Hedderson，ＴＡ・ Cox, C. J., Shaw, A. J. & Hedderson, T. A. rbcLl6cLsequencedata．Ｊ・HattoriBotLab88:79-99 sequence data. J. Hattori Bot. Lab. 88: 79-99. 2001.Z00LTheBIyophyta（mosses)：systematicand The Bryophyta (mosses): systematic and ---, ---,,Arikawa,T､,YamaguchiJ.,Higuchi, Arikawa, T., Yamaguchi, T., Higuchi, evolutionaryevolutionaryinferencesfiPomanZps4gene inferences from an Ips4 gene M.,Ｍ､，Deguchi，Ｈ、＆Seki，Ｔｌ999．Apreliminaly Deguchi, H. & Seki, T. 1999. A preliminary (cpDNA)（cpDNA)phylogeny・ＡｎｎＢｏｔ８７:l91-208 phylogeny. Ann. Bot. 87: 191-208. phylogenyphylogenyofHypnales（Musci）asinferredftom of Hypnales (Musci) as inferred from Hasegawa,Hasegawa,Ｍ，Kishino,Ｈ､＆Yano，Ｔｌ985．Dating M., Kishino, H. & Yano, T. 1985. Dating chloroplastchloroplastr6cLsequencedataBryolRes、７： rbcL sequence data. Bryol. Res. 7: ofofthehuman-apesplittingbyamolecularclockof the human-ape splitting by a molecular clock of 233-248.Z33-248 mitochondrialmitochondriaｌＤＮＡ・ｌＭｏｌ・Ｅｖｏｌ２２：l60-l74 DNA. J. Mol. Evol. 22: 160-174. Zander,Zander,ＲＨ・’993．GeneraofthePottiaceae:mosses R. H. 1993. Genera of the Pottiaceae: mosses Hedderson,ＴＡ.，Murray,ＤＪ.，Cox,Ｃ・』.＆ＮｏｗｅⅡ，Hedderson, T. A., Murray, D. J., Cox, C. J. & Nowell, ofofharshenvironments・Bull・BufftlloSoc・Namral harsh environments. Bull. Buffalo Soc. Natural Ｔ、Ｌ2004．PhylogeneticrelationshipsofT. L. 2004. Phylogenetic relationships of Science32：1-378.Science 32: 1-378. haplolepideousmosses（Dicranidae）infierredfiDmhaplolepideous mosses (Dicranidae) inferred from ---－１９９４Ｂノッocez"ﾉiospo/忽ａａｎｄＵ/eo6ﾉＷ"１．Ｉｎ1994. Blyoceuthospora and Uleoblyum. In ノアs4genesequencesSystBot29:29-41．rps4 gene sequences. Syst. Bot. 29: 29-41. Ｓｈａｗ,Ａ１，Ｃｒｕｍ,ＨＡ＆Eckel,Ｐ.Ｍ・（eds.)，Shaw, A. J., Crum, H. A. & Eckel, P. M. (eds.), Iwatsuki，Ｚ＆Suzuki，Ｔｌ998．HeisakasenruiIwatsuki, Z. & Suzuki, T. 1998. Heisakasenrui MossHoraofMexico，pp225-228・ＴｂｅＮｅｗMoss flora of Mexico, pp. 225-228. The New kenkyukoborebanashiIAdditionalcontentsinkenkyu koborebanashi. [Additional contents in YorkBotanicalGarden,ＮｅｗＹｏｒｋYork Botanical Garden, New York. studiesofcleistocarpousmosses］Fernsandstudies of cleistocarpous mosses]. Ferns and －＆During,Hll999JVeOpAoe"ｉｘ(Pottia---- & During, H. J. 1999. Neophoenix (pottia­ Mossesl5:33-38.(ｈJapanese)．Mosses 15: 33-38. (In Japanese). ceae),anewAfTicanmossgenusfbundthroughsoilceae), a new African moss genus found through soil Kiguchi,Ｈ2003．Ogawamachinosentairui.[MossesKiguchi, H. 2003. Ogawamachi no sentairui. [Mosses diasporebankanalysis、Taxon48:657-66Zdiaspore bank analysis. Taxon 48: 657-662. 0 andliverwortsinOgawa-tow､］Ogawa-machinoand liverworts in Ogawa-town]. Ogawa-machi no Accepted7.Ⅸ２００４Accepted 7. IX. 2004 shizen，shokubutsuhen，Shokubutsushunorisutqshizen, shokubutsu hen, Shokubutsushu no risuto, Sentairui,pp217-225・Ogawa-machi．（InJapa-Sentairui, pp. 217-225. Ogawa-machi. (In Japa­

１ nese） nese). 佐藤裕幸・坪田博美・山口富美夫・出口博則：ツチ~~m*·~rnM.·iliD ••*·mDM.:Y~ －，Stone,１．Ｇ.＆Iwatsuki,Ｚｌ９９６〔ノル０６ﾉＷ、I. & ---, Stone, G. Iwatsuki, Z. 1996. Uleobryum ノウエノハリゴケの系統および形態についてJ t'JIJ 1\ I) :i70)iMiJC;f3J::a:m~I::-:J~\-C 〃αgα"ｏＪｓｐｎｏｖ．（Pottiaceae）fbundinJapannaganoi sp. nov. (Pottiaceae) found in Japan. Hikobial2：１５７－l60Hikobia 12: 157-160. 西日本新産となるツチノウエノハリゴケについて[.q B *m~ c t;;'9 Y~ J t'J I J 1\ I) :1''rl:: -:J ~\-C Magombo，ＺＬＫ、2003．ThephylogenyofbasalMagombo, Z. L. K. 2003. The phylogeny of basal 仮根上結節を報告した．さらに葉緑体,bcL遺伝子のf&:fJLI:~~£i1j Gt:::.o ~ G I::~*'1