HikobiaHikobial4：１４３－１４７．２００４ 14: 143-147.2004 PhylogeneticPhylO窪eneticandmorphOlO=icalmtesⅢＩノルＣＤ〃"剛〃昭肌oiKiguchi and morphological notes on Uleobryum naganoi Kiguchi eteraL(POttiaceae,Musci)’ ale (Pottiaceae, Musci) 1 HIROYUKIHIRoYuKISATQHⅡRoMITsuBoTA,ToMIoYAMAGucHIANDHIRoNoRIDEGucH１ SATO, HIROMI TSUBOTA, TOMIO YAMAGUCHI AND HIRONORI DEGUCHI SATO,SATO,Ｈ､,TsuBoTA,Ｈ､,YAMAGucHI,Ｔ､＆DEGucHI,H2004Phylogeneticandmor- H., TSUBOTA, H., YAMAGUCHI, T. & DEGUCHI, H. 2004. Phylogenetic and mor­ phologicalphologicalnotesonU/eo6Mｲﾉ'z〃αgα"ojKiguchietα/､(Pottiaceae,Musci）Hikobia notes on Uleobryum naganoi Kiguchi et al. (Pottiaceae, Musci). Hikobia 14:l4：１４３－１４７． 143-147. UleobryumＵ/eo6/Ｗｍ〃αgα"ojKiguchiejα/､,endemictoJapanwithalimitednumberofknown naganoi Kiguchi et aI., endemic to Japan with a limited number of known locations,locations,isnewlyreportedffomShikoku,westernJapanThroughcarefUlexamina- is newly reported from Shikoku, western Japan. Through careful examina­ tionoffTeshmaterial,rhizoidalmberfbnnationisconfinnedfbrthefirsttime・The ， tion of fresh material, rhizoidal tuber formation is confirmed for the first time. The phylogeneticphylogeneticpositionofthiscleistocalpousmossisalsoassesseｄｏｎｔｈｅｂａｓｉｓｏｆｍaxi- position of this cleistocarpous moss is also assessed on the basis of maxi­ mummumlikelihoodanalysisof′bcLgenesequences、ThecuITentpositioninthePot- likelihood analysis of rbcL gene sequences. The current position in the Pot­ tiaceaetiaceaeissUpportedandacloserelationshiptoEpheme'ｗｍｓｌｐｊ""/ＯＳ"川ssuggested is supported and a close relationship to Ephemerum spinulosum is suggested. Ｈ１'わⅦkjStJro,Ｈ７ｒｏ〃ＺＭ６ｏｔａ，Tb〃ｏＹＺＪ〃αg"ｃｈｊ＆Ｈｉ７ｏ"orjDeg"cAj,Ｄｅｐ”""e"'Hiroyuki Sato, Hiromi Tsubota, Tomio Yamaguchi & Hironori Deguchi, Department of０/Ｂｊｏ/ogicα/Ｓｂｊｅ"Ce,ｏｒα`"α/eSbAoo/ｑ/肱e"Ｃｅ,肋Cs〃〃α〔ﾉﾉ､ﾉe'Ｍリノ,ＫｔＪｇα〃‐ Biological Science, Graduate School of Science, Hiroshima University, Kagami­ ｙａｍαﾉｰ3-/,Higashj-/iかCsﾉi〃α-s/ij,価'０s/i伽α７３４８５２．ﾉﾋﾘpα〃yama 1-3-/, Higashi-hiroshima-shi, Hiroshima 739-8526, Japan. arearemissingMolecularphylogeneticsmdieshave mlssmg. Molecular phylogenetic studies have Introduction beenmadetoclarifythephylogeneticpositionofbeen made to clarify the phylogenetic position of Thegenusueo6'Ｗ腕isasmallgenuscom-The genus Uleobryum is a small genus com­ somesomemosseslackingperistomes(Goffinet＆Cox mosses lacking peristomes (Goffinet & Cox prisingonlythreespecies(Kiguchiejα/,1996；prising only three species (Kiguchi et al. 1996; 2000;Goffinete/α/､1998,2002;Heddersonem/、2000; Goffinet et al. 1998, 2002; Hedderson et al. Zanderl993,1994;Zander＆Dixonl999LwithZander 1993, 1994; Zander & Dixon 1999), with 2004;Stechl999)．Theseresearchesdemonstrate2004; Stech 1999). These researches demonstrate adistributionranｇｅｉｎｔｈｅＷｅｓｔｈｄｉｅｓ，Mexicqa distribution range in the West Indies, Mexico, thatmoleculardataprovideuswithinfb､nationthat molecular data provide us with infonnation Peru,Brazil,Australia,andJapanThegenusisPeru, Brazil, Australia, and Japan. The genus is usefUltobetterunderstandsuchenigmaticcleisto-useful to better understand such enigmatic cleisto­ characterizeｄｂｙｉｔｓｓｍａｌｌｓｉｚｅ，acostawithtwocharacterized by its small size, a costa with two carpousmosses・hthispaper，rhizoidaltubercarpous mosses. In this paper, rhizoidal tuber stereidbandsandelongateadaxialepidennalstereid bands and elongate adaxial epidennal fbnnationfbrU/eo6MJm〃αgα"ｏｊｉｓｎｅｗｌｙｒｅ－fonnation for Uleobryum naganoi is newly re­ cells，spheroidalcleistocalpouscapsuleswithacells, spheroidal cleistocarpous capsules with a ｐｏｒｔｅｄａｎｄｔｈｅｐｈｙｌogeneticpositionofthisspe-ported and the phylogenetic position of this spe­ hyalineseta，transparentcapsulewallsofwhichhyaline seta, transparent capsule walls of which cieｓｉｓａｌｓｏｄｉｓｃｕｓｓｅｄｏｎｔｈｅbasisofribulosecies is also discussed on the basis of ribulose cellsaremamillose，withslightlythickenedfreecells are mamillose, with slightly thickened free 1,5-bisphosphateL5-bisphosphatecarboxylase/oxygenaselarge carboxylase/oxygenase large walls，andconic-campanulatecalyptraethatarewalls, and conic-campanulate calyptrae that are subunit（'6cL）genesequenceswithmaximumsubunit (rbcL) gene sequences with maximum roughenedapically(Zanderl993)．Ithasbeenroughened apically (Zander 1993). It has been likelihoodanalysis,togetherwithareportofnewlikelihood analysis, together with a report of new placedinthePottiaceaesinceitsestablishmentasplaced in the Pottiaceae since its establishment as habitatinJapan．habitat in Japan. anindependentgenusbyBrotherus(1906)．meo-an independent genus by Brotherus (1906). Uleo­ 6M/ｍ〃αgzJ"ojKiguchierα/､hasbeendescribedbryum naganoi Kiguchi et al. has been described MaterialsandmethodsMaterials and methods asendemictoJapanbyKiguchiem/､(1996),andas endemic to Japan by Kiguchi et al. (1996), and ischaracterizedby（１）linear-1anceolateleaves，is characterized by (1) linear-lanceolate leaves, TotalDNAwasextractedffom廿eshmaterialTotal DNA was extracted from fresh material (2)stomataonthemiddleoftheupperhalfofthe(2) stomata on the middle of the upper half of the usingthemodificationsofaphenol-chlorofbImusing the modifications of a phenol-chlorofonn capsule,ａｎｄ(3)unlobedcalyptrae，Thesystem-capsule, and (3) unlobed calyptrae. The system­ method（TsubotaeM/、1999)．PCRamplifica-method (Tsubota et al. 1999). PCR amplifica­ aticpositionofcleistocalpousmossesisequivo-atic position of cleistocarpous mosses is equivo­ tionsanddirectsequenceanalyseswerecarriedtions and direct sequence analyses were carried calwhenconclusionsarelimitedtomolphologi-cal when conclusions are limited to morphologi­ outusingstandardtechniquesDetailsandfUr-out using standard techniques. Details and fur­ calcharacters,particularlywhenmajorcharacterscal characters, particularly when major characters therinfbnnationfbrDNAextractingprotocols，ther infonnation for DNA extracting protocols, amplificationprimers，PCRprotocolsandthoseamplification primers, PCR protocols and those lContributionffomtheLaboratoryofPlantTaxonomyI Contribution from the Laboratory of Plant Taxonomy ofsequencingreactionsaredescribedinTsuboｔａof sequencing reactions are described in Tsubota andEcology,HiroshimaUniversity.Ｎ・Ser､No.５３８．and Ecology, Hiroshima University. N. Ser. No. 538. ｅｍ/､(1999,2000,2002)．et al. (1999, 2000, 2002). 1４４144 ＨｉｋｏｂｉａＶｏＬｌ４,No.２，２００４Hikobia Vol. 14, No.2, 2004 FiftysixmossspecieswereincludedintheFifty six moss species were included in the haplolepideousmossesincludingsomediplolepi-haplolepideous mosses including some diplolepi­ analysesandthesequencesweremanuallyanalyses and the sequences were manually deousmosses・Fortheanalyses,atotalofL210deous mosses. For the analyses, a total of 1,210 alignedOutgrouptaxawereselectedbasedonaligned. Outgroup taxa were selected based on sites(84.73％ｉｎ1,428bpoftheentiresequence）sites (84.73% in 1,428 bp of the entire sequence) thephylogenetichypothesisproposedbythe phylogenetic hypothesis proposed by of5676cLsequenceswereusedof 56 rbcL sequences were used. Magombo(2003)andTsubotaejaL(2003)．Magombo (2003) and Tsubota et at. (2003). lnfbnnationonthespecimensfTomwhichInfonnation on the specimens from which AphylogenetictreewasconstructedusingA phylogenetic tree was constructed using DNAwasextractedwiththeaccessionnumbersDNA was extracted with the accession numbers maximumlikelihoodcriteriａ（ＭＬ；Felsensteinmaximum likelihood criteria (ML; Felsenstein isgivenbelow：is given below: l981）AnNJtreewasobtainedusingNjdistin1981). An NJ tree was obtained using Njdist in Ｂﾉﾋﾞ)′o抑ﾉｶﾉ"ｍ〃o'WgJc""ｚｓｕｂｓｐノ叩o"Jc""’Bryoxiphium Ilorvegicum subsp. japonicum ’ MOLPHY23b3（Adachi＆Hasegawal996）MOLPHY 2.3b3 (Adachi & Hasegawa 1996) (Berggr.)Ａ・L6ve＆DL6ve,AB194720,Japan，(Berggr.) A.Love & D.Love, ABI94720, Japan, withHKY85model(Hasegawaetα/､1985),andwith HKY85 model (Hasegawa et at. 1985), and Honshu，Tottori-ken，Hino-gun，KoufU-chqMt・Honshu, Tottori-ken, Hino-gun, Koufu-cho, Mt. thebestMLtreewassearchedusi､ｇＮｕｃＭＬｉｎthe best ML tree was searched using NucML in Karasugasen，1,200ｍａｌｔ.,Ｊｕｎｅ18,2000,ＬｅｇＳ・Karasugasen, 1,200 m alt., June 18, 2000, Leg. S. ＭＯＬＰＨＹｗｉｔｈｔｈｅＮＪｔｒｅｅａｓａstarttopologyfbrMOLPHY with the NJ tree as a start topology for NogawainhbHDeguchi36501(HIRO)．Nogawa in hb. H. Deguchi 36501 (HIRO). alocalrearrangementalgorithma local rearrangement algorithm. UleobryumＵ/eo6ﾉＷ"z〃αgzJ"ojKiguchie/αL,ＡＢ１９４７１７， Ilaganoi Kiguchi et al., AB 194717, New76cLsequencesweredetenninedfbrfburNew rbcL sequences were detennined for four Japan,Shikoku,Kagawa-ken,Ayauta-guLAyakami-Japan, Shikoku, Kagawa-ken, Ayauta-gun, Ayakami­ ● species:＊Br〕ﾉoxjphj""z〃orvegzczJ"１subsp,ﾉ叩o"ノーspecies: *Bryoxiphium norvegicum subsp. japoni­ cho,NagaraLake,Ｃａ」ＯＯｍａｌｔ.[associatedwithcho, Nagara Lake, ca. 100 malt. [associated with c"腕(ABl94720),＊meo6'Ｗｍ"αgzJ"oj(AB1947cum (AB 194720), * Uleobryum naganoi (AB 1947 印/jemelw"Tspj""/Cs""７Bruch＆Schimp],Dec・Ephemerum spinulosum Bruch & Schimp.], Dec. 17),肌maco""ove耐α(ABl94718)and*Ｅｌｐｈｅ－17), Weissia controversa (AB 194718) and *Ephe­ 13,2003,'3,2003,HSato377(HIRO)． H. Sato 377 (HIRO). "'e'wmgpj"[ｲﾉCs"〃（AB194719)．Threespeciesmerum spinulosum (ABI94719). Three species Weissiaﾘﾘﾉi2jlssjaco"〃ove'ＦａＨｅｄｗ.,ＡＢ１９４７１８,Japan， controversa Hedw., AB194718, Japan, withanasteriskwereshownasanentiresequencewith an asterisk were shown as an entire sequence Honshu,Hiroshima-ken,Higashi-hiroshima-shLHonshu, Hiroshima-ken, Higashi-hiroshima-shi, 1,428l,428bplongAdatamatrixwaspreparedfbr bp long. A data matrix was prepared for ca200ma1t.,July3,2003,ＨＳａｔｏｌ６１(HIRO）ca. 200 m alt., July 3,2003, H. Sato 161 (HIRO). analysesanalysesofphylogeneticrelationｓｈｉｐｓｏｆｔｌｌｅ of phylogenetic relationships of the Ｅﾄﾞﾌﾊeme'w"ｚＳｐｊ""/Cs""ZBruch＆Ｓｃｈｉｍｐ，Ephemerum