J. Microbiol. Biotechnol. (2017), 27(0), 1–5 https://doi.org/10.4014/jmb.1712.12006 Research Article Review jmb

Fig. S1. Circular genomic map of B. s. subtilis KCTC 3135T by CLgenomics ver. 1.55. From the outer circle to the inward circle, the circle indicates rRNA and/or tRNA, reverse CDS, forward CDS, GC skew and the GC ratio.

A 2017 ⎪ Vol. 27⎪ No. 0 2Name et al.

Fig. S2. OrthoANI distance Neighbor-Joining tree with 60 strains (extended OrthoANI tree). A neighbor-joining tree based on the OrthoANI distance matrix. B. subtilis with three subspecies contains 59 genomes and S. aureus subsp. aureus DSM 20231T was used as an outgroup. The scale bar indicates the sequence divergence.

J. Microbiol. Biotechnol. Title 3

Fig. S3. The tarABD MLST NJ tree and heatmap with 60 strains (extended NJ tree). A) Phylogenetic tree using the tarABD Multilocus sequence analysis (MLSA) Neighbor-Joining (NJ) tree and B) heatmap based on the similarities of the tarABD from B. subtilis genome to the reference tar or tag genes. B. subtilis with three subspecies contains 59 genomes and S. aureus subsp. aureus DSM 20231T was used for outgroup. Bootstrap values (>70%) based on 1000 replicates are shown at branch nodes. The brand nodes also recovered maximum-likelihood (ML) and maximum-parsimony (MP) trees are marked by the filled black circles. The bar indicates nucleotide substitution rate in given length of the scale. The color indicated the tblastn nucleotide similarities to reference tar (red) or tag (green) genes.

A 2017 ⎪ Vol. 27⎪ No. 0 4Name et al.

Fig. S4. Neighbor-Joining tree with 59 strains based on concatenated sequence of seven housekeeping genes. Seven housekeeping genes (glpF, ilvD, pta, purH, pycA, rpoD, tpiA) are used for Multilocus sequence analysis (MLSA) Neighbor-Joining (NJ) phylogenetic tree. B. subtilis with three subspecies contains 59 genomes. B. subtilis subsp. inaquosorum J-5 was used for outgroup. Bootstrap values (>70%) based on 1000 replicates are shown at branch nodes. The bar indicates nucleotide substitution rate in given length of the scale.

J. Microbiol. Biotechnol. Title 5

Fig. S5. Neighbor-Joining tree based on the concatenated sequence of seven conserved genes and tarABD. Seven housekeeping genes (glpF, ilvD, pta, purH, pycA, rpoD, tpiA) and the tarABD are concatenated for Multilocus sequence analysis (MLSA) Neighbor-Joining (NJ) phylogenetic tree. B. subtilis with three subspecies contains 59 genomes. B. subtilis subsp. inaquosorum J-5 was used for outgroup. Bootstrap values (>70%) based on 1000 replicates are shown at branch nodes. The bar indicates nucleotide substitution rate in given length of the scale.

A 2017 ⎪ Vol. 27⎪ No. 0 Supplementary Table 1. Bacillus subtilis genomes used in thist study assembly accession sequence accession number Species strain name (RefSeq) (chromosome) B. subtili s subsp. subtilis 168G GCF_001703495.1 CP016852.1 168 GCF_000009045.1 AL009126.3 29R7-12 GCF_001902555.1 CP017763.1 3NA GCF_000827065.1 CP010314.1 6051-HGW GCF_000344745.1 CP003329.1 AG1839 GCF_000699525.1 CP008698.1 BAB-1 GCF_000349795.1 CP004405.1 BEST195 GCF_000209795.2 AP011541.2 BEST7003 GCF_000523045.1 AP012496.1 BS16045 GCF_001720505.1 CP017112.1 BS34A GCF_000952895.1 LN680001.1 BS38 GCF_001746575.1 CP017314.1 BS49 GCF_000953615.1 LN649259.1 BSD-2 GCF_001465815.1 CP013654.1 BSn5 GCF_000186745.1 CP002468.1 BSP1 GCF_000321395.1 CP003695.1 CGMCC 2108 GCF_001565875.1 CP014471.1 CU1050 GCF_001541905.1 CP014166.1 D12-5 GCF_001596535.1 CP014858.1 delta6 GCF_001660525.1 CP015975.1 HJ0-6 GCF_001704095.1 CP016894.1 HJ5 GCF_000973605.1 CP007173.1 HRBS-10TDI13 GCF_001747445.1 CP015222.1 JH642 substr. AG174 GCF_000699465.1 CP007800.1 JS GCF_000259365.1 CP003492.1 KCTC 1028 GCF_000971925.1 CP011115.1 KCTC 3135T GCF_001697265.1 CP015375.1 KH2 GCF_001890405.1 CP018184.1 LM 4-2 GCF_000978495.1 CP011101.1 OH 131.1 GCF_000706705.1 CP007409.1 PS832 GCF_000789295.1 CP010053.1 PY79 GCF_000497485.1 CP006881.1 QB928 GCF_000293765.1 CP003783.1 RO-NN-1 GCF_000227485.1 CP002906.1 SG6 GCF_000782835.1 CP009796.1 SZMC 6179J GCF_001604995.1 CP015004.1 TO-A JPC GCF_001037985.1 CP011882.1 TOA GCF_000737405.1 CP005997.1 UD1022 GCF_001015095.1 CP011534.1 VV2 GCF_001808235.1 CP017676.1 XF-1 GCF_000338735.1 CP004019.1 YP1 GCF_000877815.1 CP010014.1 B. subtilis subsp. spizizenii ATCC 6633 GCF_000177595.1 contigs file (incomplete) BST GCF_000743215.1 contigs file (incomplete) DV1-B-1 GCF_000245035.1 contigs file (incomplete) HUK15 GCF_001566945.1 contigs file (incomplete) JCM 2499 GCA_001312785.1a contigs file (incomplete) MJ01 GCF_001889625.1 CP018173.1 NRS 231 GCF_000816805.1 CP010434.1 RFWG1A3 GCF_000931815.1 contigs file (incomplete) RFWG1A4 GCF_000931825.1 contigs file (incomplete) RFWG4C10 GCF_000931835.1 contigs file (incomplete) RFWG5B15 GCF_000931845.1 contigs file (incomplete) T30 GCF_000959025.1 CP011051.1 TU-B-10T GCF_000227465.1 CP002905.1 W23 GCF_000146565.1 CP002183.1 B. subtilis subsp. inaquosorum DE111 GCF_001534785.1 CP013984.1 J-5 GCF_001889385.1 CP018295.1 KCTC 13429T GCF_000332645.1 contigs file (incomplete) S. aureus subsp. aureus (outgroup) DSM 20231T GCF_001027105.1 CP011526.1 a GenBank assembly accession, RefSeq assembly accession non-available. Supplementary Table 2. The tar and the tag genes tblastn similarities with 60 genomes Genes Query Target Identity Alignment length E-value Clade B_subtilis_subsp_spizizenii_T30 tarA CAC86111.1 CP011051.1_cds_BIS30_07525_1422 100 257 0 tarB CAC86110.1 CP011051.1_cds_BIS30_07530_1423 99.74 383 0 tarD CAC86112.1 CP011051.1_cds_BIS30_07520_1421 100 129 8.00E-93 tarF CAC86113.1 CP011051.1_cds_BIS30_07515_1420 100 394 0 tagA AAA22844.1 CP011051.1_cds_BIS30_07525_1422 59.53 257 3.00E-110 tagB AAA22845.1 CP011051.1_cds_BIS30_07530_1423 49.21 378 3.00E-134 tagD AAA22843.1 CP011051.1_cds_BIS30_07520_1421 76.74 129 9.00E-72 tagF NP_391453.1 CP011051.1_cds_BIS30_07515_1420 64.21 380 1.00E-178 B_subtilis_subsp_spizizenii_W23 tarA CAC86111.1 CP002183.1_cds_BSUW23_17555_3487 100 257 0 tarB CAC86110.1 CP002183.1_cds_BSUW23_17560_3488 99.74 383 0 tarD CAC86112.1 CP002183.1_cds_BSUW23_17550_3486 100 129 8.00E-93 tarF CAC86113.1 CP002183.1_cds_BSUW23_17545_3485 100 394 0 tagA AAA22844.1 CP002183.1_cds_BSUW23_17555_3487 59.53 257 4.00E-110 tagB AAA22845.1 CP002183.1_cds_BSUW23_17560_3488 49.21 378 3.00E-134 tagD AAA22843.1 CP002183.1_cds_BSUW23_17550_3486 76.74 129 1.00E-71 tagF NP_391453.1 CP002183.1_cds_BSUW23_17545_3485 64.21 380 1.00E-178 B_subtilis_subsp_spizizenii_RFWG1A4 tarA CAC86111.1 GCF_000931825.1_03517 100 257 0 tarB CAC86110.1 GCF_000931825.1_03518 99.48 383 0 tarD CAC86112.1 GCF_000931825.1_03516 100 77 8.00E-52 tarF CAC86113.1 GCF_000931825.1_03515 72.18 248 1.00E-134 tagA AAA22844.1 GCF_000931825.1_03517 59.53 257 4.00E-110 tagB AAA22845.1 GCF_000931825.1_03518 49.47 378 6.00E-136 tagD AAA22843.1 GCF_000931825.1_03516 83.12 77 7.00E-43 tagF NP_391453.1 GCF_000931825.1_03515 58.3 235 2.00E-96 B_subtilis_subsp_spizizenii_NRS_231 tarA CAC86111.1 CP010434.1_cds_SD85_17845_3413 100 257 0 tarB CAC86110.1 CP010434.1_cds_SD85_17850_3414 99.74 383 0 tarD CAC86112.1 CP010434.1_cds_SD85_17840_3412 100 129 8.00E-93 tarF CAC86113.1 CP010434.1_cds_SD85_17835_3411 100 394 0 tagA AAA22844.1 CP010434.1_cds_SD85_17845_3413 59.53 257 4.00E-110 tagB AAA22845.1 CP010434.1_cds_SD85_17850_3414 49.21 378 3.00E-134 tagD AAA22843.1 CP010434.1_cds_SD85_17840_3412 76.74 129 1.00E-71 tagF NP_391453.1 CP010434.1_cds_SD85_17835_3411 64.21 380 1.00E-178 B_subtilis_subsp_spizizenii_JCM_2499 tarA CAC86111.1 GCA_001312785.1_00738 100 257 0 tarB CAC86110.1 GCA_001312785.1_00737 99.74 383 0 tarD CAC86112.1 GCA_001312785.1_00739 100 129 8.00E-93 tarF CAC86113.1 GCA_001312785.1_00740 99.65 286 0 tagA AAA22844.1 GCA_001312785.1_00738 59.53 257 4.00E-110 tagB AAA22845.1 GCA_001312785.1_00737 49.21 378 3.00E-134 tagD AAA22843.1 GCA_001312785.1_00739 76.74 129 1.00E-71 tagF NP_391453.1 GCA_001312785.1_00740 69.96 283 4.00E-141 B_subtilis_subsp_spizizenii_BST tarA CAC86111.1 GCF_000743215.1_02629 100 257 0 tarB CAC86110.1 GCF_000743215.1_02628 99.74 383 0 tarD CAC86112.1 GCF_000743215.1_02630 100 129 8.00E-93 tarF CAC86113.1 GCF_000743215.1_02631 100 394 0 tagA AAA22844.1 GCF_000743215.1_02629 59.53 257 4.00E-110 tagB AAA22845.1 GCF_000743215.1_02628 49.21 378 3.00E-134 tagD AAA22843.1 GCF_000743215.1_02630 76.74 129 1.00E-71 tagF NP_391453.1 GCF_000743215.1_02631 64.21 380 1.00E-178 B_subtilis_subsp_spizizenii_ATCC_6633 tarA CAC86111.1 GCF_000177595.1_03132 100 257 0 tarB CAC86110.1 GCF_000177595.1_03131 99.74 383 0 tarD CAC86112.1 GCF_000177595.1_03133 100 129 8.00E-93 tarF CAC86113.1 GCF_000177595.1_03134 100 394 0 tagA AAA22844.1 GCF_000177595.1_03132 59.53 257 4.00E-110 tagB AAA22845.1 GCF_000177595.1_03131 49.21 378 3.00E-134 tagD AAA22843.1 GCF_000177595.1_03133 76.74 129 1.00E-71 tagF NP_391453.1 GCF_000177595.1_03134 64.21 380 1.00E-178 B_subtilis_subsp_spizizenii_MJ01 tarA CAC86111.1 CP018173.1_cds_BAX60_02740_545 97.28 257 0 tarB CAC86110.1 CP018173.1_cds_BAX60_02745_546 95.04 383 0 tarD CAC86112.1 CP018173.1_cds_BAX60_02735_544 96.12 129 4.00E-90 tarF CAC86113.1 CP018173.1_cds_BAX60_02730_543 64.97 394 0 tagA AAA22844.1 CP018173.1_cds_BAX60_02740_545 59.53 257 1.00E-110 tagB AAA22845.1 CP018173.1_cds_BAX60_02745_546 48.94 378 7.00E-135 tagD AAA22843.1 CP018173.1_cds_BAX60_02735_544 78.29 129 5.00E-73 tagF NP_391453.1 CP018173.1_cds_BAX60_02730_543 55.99 384 5.00E-162 B_subtilis_subsp_inaquosorum_KCTC_13429T tarA CAC86111.1 GCF_000332645.1_00225 96.89 257 0 tarB CAC86110.1 GCF_000332645.1_00226 93.99 383 0 tarD CAC86112.1 GCF_000332645.1_00224 96.12 129 2.00E-89 tarF CAC86113.1 GCF_000332645.1_00223 65.48 394 0 tagA AAA22844.1 GCF_000332645.1_00225 59.92 257 7.00E-111 tagB AAA22845.1 GCF_000332645.1_00226 49.21 380 6.00E-136 tagD AAA22843.1 GCF_000332645.1_00224 77.52 129 9.00E-73 tagF NP_391453.1 GCF_000332645.1_00223 55.21 384 9.00E-160 B_subtilis_subsp_spizizenii_HUK15 tarA CAC86111.1 GCF_001566945.1_00548 97.67 257 0 tarB CAC86110.1 GCF_001566945.1_00549 95.3 383 0 tarD CAC86112.1 GCF_001566945.1_00547 82.95 129 4.00E-79 tarF CAC86113.1 GCF_001566945.1_00552 30.1 392 1.00E-38 tagA AAA22844.1 GCF_001566945.1_00548 59.92 257 7.00E-111 tagB AAA22845.1 GCF_001566945.1_00549 48.41 378 7.00E-133 tagD AAA22843.1 GCF_001566945.1_00547 73.64 129 5.00E-69 tagF NP_391453.1 GCF_001566945.1_02251 31.25 48 5.9 B_subtilis_subsp_subtilis_HJ5 tarA CAC86111.1 CP007173.1_cds_AW03_034150_3372 94.55 257 0 tarB CAC86110.1 CP007173.1_cds_AW03_034160_3373 93.43 335 0 tarD CAC86112.1 CP007173.1_cds_AW03_034140_3371 95.35 129 6.00E-89 tarF CAC86113.1 CP007173.1_cds_AW03_034130_3370 72.54 397 0 tagA AAA22844.1 CP007173.1_cds_AW03_034150_3372 59.92 257 5.00E-110 tagB AAA22845.1 CP007173.1_cds_AW03_034160_3373 48.79 330 6.00E-117 tagD AAA22843.1 CP007173.1_cds_AW03_034140_3371 77.52 129 7.00E-73 tagF NP_391453.1 CP007173.1_cds_AW03_034130_3370 55.47 384 5.00E-157 B_subtilis_subsp_subtilis_XF-1 tarA CAC86111.1 CP004019.1_cds_C663_3466_3311 94.55 257 0 tarB CAC86110.1 CP004019.1_cds_C663_3467_3312 93.3 373 0 tarD CAC86112.1 CP004019.1_cds_C663_3465_3310 95.35 129 5.00E-89 tarF CAC86113.1 CP004019.1_cds_C663_3464_3309 72.54 397 0 A tagA AAA22844.1 CP004019.1_cds_C663_3466_3311 59.92 257 7.00E-110 tagB AAA22845.1 CP004019.1_cds_C663_3467_3312 49.59 367 6.00E-133 tagD AAA22843.1 CP004019.1_cds_C663_3465_3310 77.52 129 6.00E-73 tagF NP_391453.1 CP004019.1_cds_C663_3464_3309 55.47 384 5.00E-157 B_subtilis_subsp_subtilis_BSD-2 tarA CAC86111.1 CP013654.1_cds_AT706_00735_133 94.55 257 0 tarB CAC86110.1 CP013654.1_cds_AT706_00730_132 93.21 383 0 tarD CAC86112.1 CP013654.1_cds_AT706_00740_134 95.35 129 6.00E-89 tarF CAC86113.1 CP013654.1_cds_AT706_00745_135 72.54 397 0 tagA AAA22844.1 CP013654.1_cds_AT706_00735_133 59.92 257 5.00E-110 tagB AAA22845.1 CP013654.1_cds_AT706_00730_132 48.94 378 8.00E-134 tagD AAA22843.1 CP013654.1_cds_AT706_00740_134 77.52 129 7.00E-73 tagF NP_391453.1 CP013654.1_cds_AT706_00745_135 55.47 384 5.00E-157 B_subtilis_subsp_subtilis_BAB-1 tarA CAC86111.1 CP004405.1_cds_I653_17435_3452 94.55 257 0 tarB CAC86110.1 CP004405.1_cds_I653_17440_3453 93.21 383 0 tarD CAC86112.1 CP004405.1_cds_I653_17430_3451 95.35 129 6.00E-89 tarF CAC86113.1 CP004405.1_cds_I653_17425_3450 72.54 397 0 tagA AAA22844.1 CP004405.1_cds_I653_17435_3452 59.92 257 5.00E-110 tagB AAA22845.1 CP004405.1_cds_I653_17440_3453 48.94 378 8.00E-134 tagD AAA22843.1 CP004405.1_cds_I653_17430_3451 77.52 129 7.00E-73 tagF NP_391453.1 CP004405.1_cds_I653_17425_3450 55.47 384 5.00E-157 B_subtilis_subsp_subtilis_UD1022 tarA CAC86111.1 CP011534.1_cds_ABA10_17865_3399 94.55 257 0 tarB CAC86110.1 CP011534.1_cds_ABA10_17870_3400 93.99 383 0 tarD CAC86112.1 CP011534.1_cds_ABA10_17860_3398 94.57 129 2.00E-88 tarF CAC86113.1 CP011534.1_cds_ABA10_17855_3397 64.72 394 0 tagA AAA22844.1 CP011534.1_cds_ABA10_17865_3399 59.92 257 5.00E-110 tagB AAA22845.1 CP011534.1_cds_ABA10_17870_3400 48.94 378 4.00E-134 tagD AAA22843.1 CP011534.1_cds_ABA10_17860_3398 78.29 129 9.00E-74 tagF NP_391453.1 CP011534.1_cds_ABA10_17855_3397 54.95 384 4.00E-160 B_subtilis_subsp_subtilis_YP1 tarA CAC86111.1 CP010014.1_cds_QF06_16280_3158 94.55 257 0 tarB CAC86110.1 CP010014.1_cds_QF06_16285_3159 92.43 383 0 tarD CAC86112.1 CP010014.1_cds_QF06_16275_3157 95.35 129 7.00E-89 tarF CAC86113.1 CP010014.1_cds_QF06_16270_3156 64.72 394 0 tagA AAA22844.1 CP010014.1_cds_QF06_16280_3158 59.53 257 1.00E-109 tagB AAA22845.1 CP010014.1_cds_QF06_16285_3159 48.94 378 2.00E-133 tagD AAA22843.1 CP010014.1_cds_QF06_16275_3157 77.52 129 9.00E-73 tagF NP_391453.1 CP010014.1_cds_QF06_16270_3156 54.95 384 4.00E-160 B_subtilis_subsp_subtilis_HJ0-6 tarA CAC86111.1 CP016894.1_cds_BSHJ0_03808_3696 94.16 257 0 tarB CAC86110.1 CP016894.1_cds_BSHJ0_03809_3697 92.95 383 0 tarD CAC86112.1 CP016894.1_cds_BSHJ0_03807_3695 96.12 129 2.00E-89 tarF CAC86113.1 CP016894.1_cds_BSHJ0_03806_3694 93.15 394 0 tagA AAA22844.1 CP016894.1_cds_BSHJ0_03808_3696 59.53 257 1.00E-109 tagB AAA22845.1 CP016894.1_cds_BSHJ0_03809_3697 48.94 378 6.00E-134 tagD AAA22843.1 CP016894.1_cds_BSHJ0_03807_3695 76.74 129 3.00E-72 tagF NP_391453.1 CP016894.1_cds_BSHJ0_03806_3694 63.28 384 5.00E-179 B_subtilis_subsp_subtilis_OH_131.1 tarA CAC86111.1 CP007409.1_cds_Q433_19585_3349 94.16 257 0 tarB CAC86110.1 CP007409.1_cds_Q433_19590_3350 92.95 383 0 tarD CAC86112.1 CP007409.1_cds_Q433_19580_3348 96.12 129 1.00E-89 tarF CAC86113.1 CP007409.1_cds_Q433_19575_3347 93.15 394 0 tagA AAA22844.1 CP007409.1_cds_Q433_19585_3349 59.53 257 1.00E-109 tagB AAA22845.1 CP007409.1_cds_Q433_19590_3350 48.94 378 5.00E-134 tagD AAA22843.1 CP007409.1_cds_Q433_19580_3348 76.74 129 3.00E-72 tagF NP_391453.1 CP007409.1_cds_Q433_19575_3347 63.28 384 5.00E-179 B_subtilis_subsp_subtilis_BSP1 tarA CAC86111.1 CP003695.1_cds_A7A1_1145_507 94.55 257 0 tarB CAC86110.1 CP003695.1_cds_A7A1_1144_506 92.69 383 0 tarD CAC86112.1 CP003695.1_cds_A7A1_1146_508 96.1 77 6.00E-50 tarF CAC86113.1 CP003695.1_cds_A7A1_1147_509 64.72 394 0 tagA AAA22844.1 CP003695.1_cds_A7A1_1145_507 59.53 257 1.00E-109 tagB AAA22845.1 CP003695.1_cds_A7A1_1144_506 48.94 378 1.00E-133 tagD AAA22843.1 CP003695.1_cds_A7A1_1146_508 83.12 77 1.00E-43 tagF NP_391453.1 CP003695.1_cds_A7A1_1147_509 54.95 384 4.00E-160 B_subtilis_subsp_subtilis_D12-5 tarA CAC86111.1 CP014858.1_cds_KHRBS_02840_564 94.55 257 0 tarB CAC86110.1 CP014858.1_cds_KHRBS_02835_563 92.69 383 0 tarD CAC86112.1 CP014858.1_cds_KHRBS_02845_565 95.35 129 2.00E-89 tarF CAC86113.1 CP014858.1_cds_KHRBS_02850_566 64.72 394 0 tagA AAA22844.1 CP014858.1_cds_KHRBS_02840_564 59.53 257 1.00E-109 tagB AAA22845.1 CP014858.1_cds_KHRBS_02835_563 48.94 378 1.00E-133 tagD AAA22843.1 CP014858.1_cds_KHRBS_02845_565 76.74 129 3.00E-72 tagF NP_391453.1 CP014858.1_cds_KHRBS_02850_566 54.95 384 3.00E-160 B_subtilis_subsp_subtilis_JS tarA CAC86111.1 CP003492.1_cds_MY9_3627_3619 94.94 257 0 tarB CAC86110.1 CP003492.1_cds_MY9_3629_3621 92.15 382 0 tarD CAC86112.1 CP003492.1_cds_MY9_3626_3618 96.9 129 2.00E-90 tarF CAC86113.1 CP003492.1_cds_MY9_3625_3617 69.44 396 0 tagA AAA22844.1 CP003492.1_cds_MY9_3627_3619 60.31 257 8.00E-111 tagB AAA22845.1 CP003492.1_cds_MY9_3629_3621 49.47 378 1.00E-134 tagD AAA22843.1 CP003492.1_cds_MY9_3626_3618 75.97 129 2.00E-71 tagF NP_391453.1 CP003492.1_cds_MY9_3625_3617 60.94 384 2.00E-173 B_subtilis_subsp_inaquosorum_DE111 tarA CAC86111.1 CP013984.1_cds_AN935_18015_3558 96.89 257 0 tarB CAC86110.1 CP013984.1_cds_AN935_18020_3559 93.73 383 0 tarD CAC86112.1 CP013984.1_cds_AN935_18010_3557 75.19 129 3.00E-72 tarF CAC86113.1 CP013984.1_cds_AN935_18005_3556 58.46 390 9.00E-173 tagA AAA22844.1 CP013984.1_cds_AN935_18015_3558 59.92 257 7.00E-111 tagB AAA22845.1 CP013984.1_cds_AN935_18020_3559 49.87 379 9.00E-137 tagD AAA22843.1 CP013984.1_cds_AN935_18010_3557 90.7 129 1.00E-83 tagF NP_391453.1 CP013984.1_cds_AN935_18005_3556 56.85 387 2.00E-165 B_subtilis_subsp_spizizenii_TU-B-10T tarA CAC86111.1 CP002905.1_cds_GYO_3930_3726 97.67 257 0 tarB CAC86110.1 CP002905.1_cds_GYO_3932_3728 94.78 383 0 tarD CAC86112.1 CP002905.1_cds_GYO_3929_3725 75.19 129 2.00E-71 tarF CAC86113.1 CP002905.1_cds_GYO_3928_3724 59.9 389 6.00E-168 tagA AAA22844.1 CP002905.1_cds_GYO_3930_3726 59.92 257 7.00E-111 tagB AAA22845.1 CP002905.1_cds_GYO_3932_3728 47.88 378 6.00E-132 tagD AAA22843.1 CP002905.1_cds_GYO_3929_3725 89.15 129 3.00E-81 tagF NP_391453.1 CP002905.1_cds_GYO_3928_3724 54.1 390 2.00E-148 B_subtilis_subsp_subtilis_BEST195 tarA CAC86111.1 AP011541.2_cds_BAI87217.1_3872 60.16 256 7.00E-116 tarB CAC86110.1 AP011541.2_cds_BAI87218.2_3873 51.34 372 9.00E-129 tarD CAC86112.1 AP011541.2_cds_BAI87216.1_3871 75.97 129 2.00E-73 tarF CAC86113.1 AP011541.2_cds_BAI87215.1_3870 62.34 385 3.00E-178 tagA AAA22844.1 AP011541.2_cds_BAI87217.1_3872 62.5 256 3.00E-122 tagB AAA22845.1 AP011541.2_cds_BAI87218.2_3873 52.62 363 2.00E-140 tagD AAA22843.1 AP011541.2_cds_BAI87216.1_3871 93.8 129 8.00E-87 tagF NP_391453.1 AP011541.2_cds_BAI87215.1_3870 57.92 644 0 B_subtilis_subsp_subtilis_BS38 tarA CAC86111.1 CP017314.1_cds_BSBS38_03699_3587 60.16 256 7.00E-116 tarB CAC86110.1 CP017314.1_cds_BSBS38_03700_3588 50.53 380 1.00E-129 tarD CAC86112.1 CP017314.1_cds_BSBS38_03698_3586 75.97 129 2.00E-73 tarF CAC86113.1 CP017314.1_cds_BSBS38_03697_3585 62.34 385 3.00E-178 tagA AAA22844.1 CP017314.1_cds_BSBS38_03699_3587 62.5 256 3.00E-122 tagB AAA22845.1 CP017314.1_cds_BSBS38_03700_3588 52.52 377 9.00E-144 tagD AAA22843.1 CP017314.1_cds_BSBS38_03698_3586 93.8 129 8.00E-87 tagF NP_391453.1 CP017314.1_cds_BSBS38_03697_3585 57.92 644 0 B_subtilis_subsp_subtilis_29R7-12 tarA CAC86111.1 CP017763.1_cds_BKP58_15250_2959 60.16 256 7.00E-116 tarB CAC86110.1 CP017763.1_cds_BKP58_15245_2958 50.53 380 1.00E-129 tarD CAC86112.1 CP017763.1_cds_BKP58_15255_2960 75.97 129 2.00E-73 tarF CAC86113.1 CP017763.1_cds_BKP58_15260_2961 62.34 385 3.00E-178 tagA AAA22844.1 CP017763.1_cds_BKP58_15250_2959 62.5 256 3.00E-122 tagB AAA22845.1 CP017763.1_cds_BKP58_15245_2958 52.52 377 9.00E-144 tagD AAA22843.1 CP017763.1_cds_BKP58_15255_2960 93.8 129 1.00E-86 tagF NP_391453.1 CP017763.1_cds_BKP58_15260_2961 57.92 644 0 B_subtilis_subsp_subtilis_CGMCC_2108 tarA CAC86111.1 CP014471.1_cds_AWV81_19085_3699 60.16 256 7.00E-116 tarB CAC86110.1 CP014471.1_cds_AWV81_19090_3700 50.53 380 1.00E-129 tarD CAC86112.1 CP014471.1_cds_AWV81_19080_3698 75.97 129 2.00E-73 tarF CAC86113.1 CP014471.1_cds_AWV81_19075_3697 62.34 385 3.00E-178 tagA AAA22844.1 CP014471.1_cds_AWV81_19085_3699 62.5 256 3.00E-122 tagB AAA22845.1 CP014471.1_cds_AWV81_19090_3700 52.52 377 9.00E-144 tagD AAA22843.1 CP014471.1_cds_AWV81_19080_3698 93.8 129 1.00E-86 tagF NP_391453.1 CP014471.1_cds_AWV81_19075_3697 57.92 644 0 B_subtilis_subsp_subtilis_HRBS-10TDI13 tarA CAC86111.1 CP015222.1_cds_A4A60_19370_3756 60.16 256 7.00E-116 tarB CAC86110.1 CP015222.1_cds_A4A60_19375_3757 50.53 380 1.00E-129 tarD CAC86112.1 CP015222.1_cds_A4A60_19365_3755 75.97 129 2.00E-73 tarF CAC86113.1 CP015222.1_cds_A4A60_19360_3754 62.34 385 3.00E-178 tagA AAA22844.1 CP015222.1_cds_A4A60_19370_3756 62.5 256 3.00E-122 tagB AAA22845.1 CP015222.1_cds_A4A60_19375_3757 52.52 377 9.00E-144 tagD AAA22843.1 CP015222.1_cds_A4A60_19365_3755 93.8 129 1.00E-86 tagF NP_391453.1 CP015222.1_cds_A4A60_19360_3754 57.92 644 0 B_subtilis_subsp_subtilis_KH2 tarA CAC86111.1 CP018184.1_cds_BSR08_15450_3035 60.16 256 7.00E-116 tarB CAC86110.1 CP018184.1_cds_BSR08_15455_3036 50.53 380 1.00E-129 tarD CAC86112.1 CP018184.1_cds_BSR08_15445_3034 75.97 129 2.00E-73 tarF CAC86113.1 CP018184.1_cds_BSR08_15440_3033 62.34 385 3.00E-178 tagA AAA22844.1 CP018184.1_cds_BSR08_15450_3035 62.5 256 3.00E-122 tagB AAA22845.1 CP018184.1_cds_BSR08_15455_3036 52.52 377 9.00E-144 tagD AAA22843.1 CP018184.1_cds_BSR08_15445_3034 93.8 129 1.00E-86 tagF NP_391453.1 CP018184.1_cds_BSR08_15440_3033 57.92 644 0 B_subtilis_subsp_inaquosorum_J-5 tarA CAC86111.1 CP018295.1_cds_BHE96_18005_3486 53.31 257 2.00E-103 tarB CAC86110.1 CP018295.1_cds_BHE96_18010_3487 52.86 384 2.00E-135 tarD CAC86112.1 CP018295.1_cds_BHE96_18000_3485 75.19 129 7.00E-72 tarF CAC86113.1 CP018295.1_cds_BHE96_17990_3483 63.78 392 1.00E-180 tagA AAA22844.1 CP018295.1_cds_BHE96_18005_3486 66.41 256 9.00E-132 tagB AAA22845.1 CP018295.1_cds_BHE96_18010_3487 58.01 381 5.00E-163 tagD AAA22843.1 CP018295.1_cds_BHE96_18000_3485 91.47 129 4.00E-86 tagF NP_391453.1 CP018295.1_cds_BHE96_17990_3483 75.62 722 0 B_subtilis_subsp_spizizenii_RFWG1A3 tarA CAC86111.1 GCF_000931815.1_03474 62.92 240 4.00E-111 tarB CAC86110.1 GCF_000931815.1_03476 53.58 293 4.00E-107 tarD CAC86112.1 GCF_000931815.1_03473 76.74 129 1.00E-71 tarF CAC86113.1 GCF_000931815.1_03471 61.98 384 1.00E-174 tagA AAA22844.1 GCF_000931815.1_03474 94.61 241 1.00E-172 tagB AAA22845.1 GCF_000931815.1_03476 85.71 294 0 tagD AAA22843.1 GCF_000931815.1_03473 100 129 2.00E-92 tagF NP_391453.1 GCF_000931815.1_03471 93.98 731 0 B_subtilis_subsp_spizizenii_RFWG4C10 tarA CAC86111.1 GCF_000931835.1_03473 62.92 240 4.00E-111 tarB CAC86110.1 GCF_000931835.1_03474 51.32 378 2.00E-135 tarD CAC86112.1 GCF_000931835.1_03472 76.74 129 1.00E-71 tarF CAC86113.1 GCF_000931835.1_03470 61.98 384 9.00E-175 tagA AAA22844.1 GCF_000931835.1_03473 94.26 244 3.00E-174 tagB AAA22845.1 GCF_000931835.1_03474 87.14 381 0 tagD AAA22843.1 GCF_000931835.1_03472 100 129 2.00E-92 tagF NP_391453.1 GCF_000931835.1_03470 93.98 731 0 B_subtilis_subsp_spizizenii_RFWG5B15 tarA CAC86111.1 GCF_000931845.1_03452 60.31 257 7.00E-112 tarB CAC86110.1 GCF_000931845.1_03453 51.32 378 2.00E-135 tarD CAC86112.1 GCF_000931845.1_03451 76.74 129 1.00E-71 tarF CAC86113.1 GCF_000931845.1_03448 61.98 384 9.00E-175 tagA AAA22844.1 GCF_000931845.1_03452 95.31 256 0 tagB AAA22845.1 GCF_000931845.1_03453 87.14 381 0 tagD AAA22843.1 GCF_000931845.1_03451 100 129 2.00E-92 tagF NP_391453.1 GCF_000931845.1_03448 93.98 731 0 B_subtilis_subsp_spizizenii_DV1-B-1 tarA CAC86111.1 GCF_000245035.1_00220 59.92 257 5.00E-112 tarB CAC86110.1 GCF_000245035.1_00221 49.35 383 6.00E-133 tarD CAC86112.1 GCF_000245035.1_00219 76.74 129 1.00E-71 tarF CAC86113.1 GCF_000245035.1_00217 62.24 384 6.00E-176 tagA AAA22844.1 GCF_000245035.1_00220 95.31 256 0 tagB AAA22845.1 GCF_000245035.1_00221 87.14 381 0 tagD AAA22843.1 GCF_000245035.1_00219 100 129 2.00E-92 tagF NP_391453.1 GCF_000245035.1_00217 92.48 731 0 B_subtilis_subsp_subtilis_RO-NN-1 tarA CAC86111.1 CP002906.1_cds_I33_3704_3569 59.53 257 4.00E-110 tarB CAC86110.1 CP002906.1_cds_I33_3705_3570 50.14 367 2.00E-131 tarD CAC86112.1 CP002906.1_cds_I33_3703_3568 76.74 129 1.00E-71 tarF CAC86113.1 CP002906.1_cds_I33_3701_3566 63.43 391 2.00E-178 tagA AAA22844.1 CP002906.1_cds_I33_3704_3569 100 256 0 tagB AAA22845.1 CP002906.1_cds_I33_3705_3570 99.73 370 0 tagD AAA22843.1 CP002906.1_cds_I33_3703_3568 100 129 2.00E-92 tagF NP_391453.1 CP002906.1_cds_I33_3701_3566 99.6 746 0 B_subtilis_subsp_subtilis_TOA tarA CAC86111.1 CP005997.1_cds_M036_18280_3538 59.53 257 4.00E-110 tarB CAC86110.1 CP005997.1_cds_M036_18285_3539 49.21 378 3.00E-132 tarD CAC86112.1 CP005997.1_cds_M036_18275_3537 76.74 129 1.00E-71 tarF CAC86113.1 CP005997.1_cds_M036_18265_3535 63.43 391 2.00E-178 tagA AAA22844.1 CP005997.1_cds_M036_18280_3538 100 256 0 tagB AAA22845.1 CP005997.1_cds_M036_18285_3539 100 381 0 tagD AAA22843.1 CP005997.1_cds_M036_18275_3537 100 129 2.00E-92 tagF NP_391453.1 CP005997.1_cds_M036_18265_3535 100 746 0 B_subtilis_subsp_subtilis_TO-A_JPC tarA CAC86111.1 CP011882.1_cds_ABU16_0427_427 59.53 257 4.00E-110 tarB CAC86110.1 CP011882.1_cds_ABU16_0428_428 49.21 378 3.00E-132 tarD CAC86112.1 CP011882.1_cds_ABU16_0426_426 76.74 129 1.00E-71 tarF CAC86113.1 CP011882.1_cds_ABU16_0424_424 63.43 391 2.00E-178 tagA AAA22844.1 CP011882.1_cds_ABU16_0427_427 100 256 0 tagB AAA22845.1 CP011882.1_cds_ABU16_0428_428 100 381 0 tagD AAA22843.1 CP011882.1_cds_ABU16_0426_426 100 129 2.00E-92 tagF NP_391453.1 CP011882.1_cds_ABU16_0424_424 100 746 0 B_subtilis_subsp_subtilis_168G tarA CAC86111.1 CP016852.1_cds_BFI33_19360_3755 59.53 257 4.00E-110 tarB CAC86110.1 CP016852.1_cds_BFI33_19365_3756 49.21 378 3.00E-132 tarD CAC86112.1 CP016852.1_cds_BFI33_19355_3754 76.74 129 1.00E-71 tarF CAC86113.1 CP016852.1_cds_BFI33_19345_3752 63.43 391 2.00E-178 tagA AAA22844.1 CP016852.1_cds_BFI33_19360_3755 100 256 0 tagB AAA22845.1 CP016852.1_cds_BFI33_19365_3756 100 381 0 tagD AAA22843.1 CP016852.1_cds_BFI33_19355_3754 100 129 2.00E-92 tagF NP_391453.1 CP016852.1_cds_BFI33_19345_3752 100 746 0 B_subtilis_subsp_subtilis_168 tarA CAC86111.1 AL009126.3_cds_CAB15592.1_3703 59.53 257 4.00E-110 tarB CAC86110.1 AL009126.3_cds_CAB15593.1_3704 49.21 378 3.00E-132 tarD CAC86112.1 AL009126.3_cds_CAB15591.1_3702 76.74 129 1.00E-71 tarF CAC86113.1 AL009126.3_cds_CAB15589.1_3700 63.43 391 2.00E-178 tagA AAA22844.1 AL009126.3_cds_CAB15592.1_3703 100 256 0 tagB AAA22845.1 AL009126.3_cds_CAB15593.1_3704 100 381 0 tagD AAA22843.1 AL009126.3_cds_CAB15591.1_3702 100 129 2.00E-92 tagF NP_391453.1 AL009126.3_cds_CAB15589.1_3700 100 746 0 B_subtilis_subsp_subtilis_3NA tarA CAC86111.1 CP010314.1_cds_RP72_19255_3721 59.53 257 4.00E-110 tarB CAC86110.1 CP010314.1_cds_RP72_19260_3722 48.94 378 4.00E-131 tarD CAC86112.1 CP010314.1_cds_RP72_19250_3720 76.74 129 1.00E-71 tarF CAC86113.1 CP010314.1_cds_RP72_19240_3718 63.43 391 2.00E-178 tagA AAA22844.1 CP010314.1_cds_RP72_19255_3721 100 256 0 tagB AAA22845.1 CP010314.1_cds_RP72_19260_3722 99.74 381 0 tagD AAA22843.1 CP010314.1_cds_RP72_19250_3720 100 129 2.00E-92 tagF NP_391453.1 CP010314.1_cds_RP72_19240_3718 100 746 0 B_subtilis_subsp_subtilis_6051-HGW tarA CAC86111.1 CP003329.1_cds_BSU6051_35750_3643 59.53 257 4.00E-110 tarB CAC86110.1 CP003329.1_cds_BSU6051_35760_3644 49.21 378 3.00E-132 tarD CAC86112.1 CP003329.1_cds_BSU6051_35740_3642 76.74 129 1.00E-71 tarF CAC86113.1 CP003329.1_cds_BSU6051_35720_3640 63.43 391 2.00E-178 tagA AAA22844.1 CP003329.1_cds_BSU6051_35750_3643 100 256 0 tagB AAA22845.1 CP003329.1_cds_BSU6051_35760_3644 100 381 0 tagD AAA22843.1 CP003329.1_cds_BSU6051_35740_3642 100 129 2.00E-92 tagF NP_391453.1 CP003329.1_cds_BSU6051_35720_3640 100 746 0 B_subtilis_subsp_subtilis_AG1839 tarA CAC86111.1 CP008698.1_cds_BSUB_03808_3684 59.53 257 4.00E-110 tarB CAC86110.1 CP008698.1_cds_BSUB_03809_3685 49.21 378 3.00E-132 tarD CAC86112.1 CP008698.1_cds_BSUB_03807_3683 76.74 129 1.00E-71 tarF CAC86113.1 CP008698.1_cds_BSUB_03805_3681 63.43 391 2.00E-178 tagA AAA22844.1 CP008698.1_cds_BSUB_03808_3684 100 256 0 tagB AAA22845.1 CP008698.1_cds_BSUB_03809_3685 100 381 0 tagD AAA22843.1 CP008698.1_cds_BSUB_03807_3683 100 129 2.00E-92 tagF NP_391453.1 CP008698.1_cds_BSUB_03805_3681 100 746 0 B_subtilis_subsp_subtilis_BEST7003 tarA CAC86111.1 AP012496.1_cds_BAM59666.1_3465 59.53 257 4.00E-110 tarB CAC86110.1 AP012496.1_cds_BAM59667.1_3466 49.21 378 3.00E-132 tarD CAC86112.1 AP012496.1_cds_BAM59665.1_3464 76.74 129 1.00E-71 tarF CAC86113.1 AP012496.1_cds_BAM59663.1_3462 63.43 391 2.00E-178 tagA AAA22844.1 AP012496.1_cds_BAM59666.1_3465 100 256 0 tagB AAA22845.1 AP012496.1_cds_BAM59667.1_3466 100 381 0 tagD AAA22843.1 AP012496.1_cds_BAM59665.1_3464 100 129 2.00E-92 tagF NP_391453.1 AP012496.1_cds_BAM59663.1_3462 100 746 0 B_subtilis_subsp_subtilis_BS16045 tarA CAC86111.1 CP017112.1_cds_BS16045_03760_3648 66.67 102 3.00E-44 tarB CAC86110.1 CP017112.1_cds_BS16045_03761_3649 47.42 213 3.00E-70 tarD CAC86112.1 CP017112.1_cds_BS16045_03758_3646 76.74 129 1.00E-71 tarF CAC86113.1 CP017112.1_cds_BS16045_03755_3643 63.43 391 1.00E-178 tagA AAA22844.1 CP017112.1_cds_BS16045_03760_3648 100 101 8.00E-72 tagB AAA22845.1 CP017112.1_cds_BS16045_03761_3649 100 213 5.00E-154 tagD AAA22843.1 CP017112.1_cds_BS16045_03758_3646 100 129 2.00E-92 tagF NP_391453.1 CP017112.1_cds_BS16045_03755_3643 100 721 0 B B_subtilis_subsp_subtilis_BS34A tarA CAC86111.1 LN680001.1_cds_CEJ79239.1_3692 59.53 257 4.00E-110 tarB CAC86110.1 LN680001.1_cds_CEJ79240.1_3693 49.21 378 3.00E-132 tarD CAC86112.1 LN680001.1_cds_CEJ79238.1_3691 76.74 129 1.00E-71 tarF CAC86113.1 LN680001.1_cds_CEJ79236.1_3689 63.43 391 2.00E-178 tagA AAA22844.1 LN680001.1_cds_CEJ79239.1_3692 100 256 0 tagB AAA22845.1 LN680001.1_cds_CEJ79240.1_3693 100 381 0 tagD AAA22843.1 LN680001.1_cds_CEJ79238.1_3691 100 129 2.00E-92 tagF NP_391453.1 LN680001.1_cds_CEJ79236.1_3689 100 746 0 B_subtilis_subsp_subtilis_BS49 tarA CAC86111.1 LN649259.1_cds_CEI59382.1_3709 59.53 257 4.00E-110 tarB CAC86110.1 LN649259.1_cds_CEI59383.1_3710 49.21 378 3.00E-132 tarD CAC86112.1 LN649259.1_cds_CEI59381.1_3708 76.74 129 1.00E-71 tarF CAC86113.1 LN649259.1_cds_CEI59379.1_3706 63.43 391 2.00E-178 tagA AAA22844.1 LN649259.1_cds_CEI59382.1_3709 100 256 0 tagB AAA22845.1 LN649259.1_cds_CEI59383.1_3710 100 381 0 tagD AAA22843.1 LN649259.1_cds_CEI59381.1_3708 100 129 2.00E-92 tagF NP_391453.1 LN649259.1_cds_CEI59379.1_3706 100 746 0 B_subtilis_subsp_subtilis_CU1050 tarA CAC86111.1 CP014166.1_cds_AWM80_18240_3531 59.53 257 4.00E-110 tarB CAC86110.1 CP014166.1_cds_AWM80_18245_3532 49.21 378 3.00E-132 tarD CAC86112.1 CP014166.1_cds_AWM80_18235_3530 76.74 129 1.00E-71 tarF CAC86113.1 CP014166.1_cds_AWM80_18225_3528 63.43 391 2.00E-178 tagA AAA22844.1 CP014166.1_cds_AWM80_18240_3531 100 256 0 tagB AAA22845.1 CP014166.1_cds_AWM80_18245_3532 100 381 0 tagD AAA22843.1 CP014166.1_cds_AWM80_18235_3530 100 129 2.00E-92 tagF NP_391453.1 CP014166.1_cds_AWM80_18225_3528 100 746 0 B_subtilis_subsp_subtilis_delta6 tarA CAC86111.1 CP015975.1_cds_A8O17_17490_3381 59.53 257 4.00E-110 tarB CAC86110.1 CP015975.1_cds_A8O17_17495_3382 49.21 378 3.00E-132 tarD CAC86112.1 CP015975.1_cds_A8O17_17485_3380 76.74 129 9.00E-72 tarF CAC86113.1 CP015975.1_cds_A8O17_17475_3378 63.43 391 2.00E-178 tagA AAA22844.1 CP015975.1_cds_A8O17_17490_3381 100 256 0 tagB AAA22845.1 CP015975.1_cds_A8O17_17495_3382 100 381 0 tagD AAA22843.1 CP015975.1_cds_A8O17_17485_3380 100 129 2.00E-92 tagF NP_391453.1 CP015975.1_cds_A8O17_17475_3378 100 746 0 B_subtilis_subsp_subtilis_JH642_substr_AG174 tarA CAC86111.1 CP007800.1_cds_BSUA_03808_3680 59.53 257 4.00E-110 tarB CAC86110.1 CP007800.1_cds_BSUA_03809_3681 49.21 378 3.00E-132 tarD CAC86112.1 CP007800.1_cds_BSUA_03807_3679 76.74 129 1.00E-71 tarF CAC86113.1 CP007800.1_cds_BSUA_03805_3677 63.43 391 2.00E-178 tagA AAA22844.1 CP007800.1_cds_BSUA_03808_3680 100 256 0 tagB AAA22845.1 CP007800.1_cds_BSUA_03809_3681 100 381 0 tagD AAA22843.1 CP007800.1_cds_BSUA_03807_3679 100 129 2.00E-92 tagF NP_391453.1 CP007800.1_cds_BSUA_03805_3677 100 746 0 B_subtilis_subsp_subtilis_KCTC_1028 tarA CAC86111.1 CP011115.1_cds_O7A_19360_3747 59.53 257 4.00E-110 tarB CAC86110.1 CP011115.1_cds_O7A_19365_3748 49.21 378 3.00E-132 tarD CAC86112.1 CP011115.1_cds_O7A_19355_3746 76.74 129 1.00E-71 tarF CAC86113.1 CP011115.1_cds_O7A_19345_3744 63.43 391 2.00E-178 tagA AAA22844.1 CP011115.1_cds_O7A_19360_3747 100 256 0 tagB AAA22845.1 CP011115.1_cds_O7A_19365_3748 100 381 0 tagD AAA22843.1 CP011115.1_cds_O7A_19355_3746 100 129 2.00E-92 tagF NP_391453.1 CP011115.1_cds_O7A_19345_3744 100 746 0 B_subtilis_subsp_subtilis_KCTC_3135T tarA CAC86111.1 CP015375.1_cds_AS891_04860_919 59.53 257 4.00E-110 tarB CAC86110.1 CP015375.1_cds_AS891_04855_918 49.21 378 3.00E-132 tarD CAC86112.1 CP015375.1_cds_AS891_04865_920 76.74 129 1.00E-71 tarF CAC86113.1 CP015375.1_cds_AS891_04875_922 63.43 391 2.00E-178 tagA AAA22844.1 CP015375.1_cds_AS891_04860_919 100 256 0 tagB AAA22845.1 CP015375.1_cds_AS891_04855_918 100 381 0 tagD AAA22843.1 CP015375.1_cds_AS891_04865_920 100 129 2.00E-92 tagF NP_391453.1 CP015375.1_cds_AS891_04875_922 100 746 0 B_subtilis_subsp_subtilis_LM_4-2 tarA CAC86111.1 CP011101.1_cds_BsLM_3591_3587 59.53 257 4.00E-110 tarB CAC86110.1 CP011101.1_cds_BsLM_3592_3588 49.21 378 3.00E-132 tarD CAC86112.1 CP011101.1_cds_BsLM_3590_3586 76.74 129 1.00E-71 tarF CAC86113.1 CP011101.1_cds_BsLM_3588_3584 62.76 392 2.00E-178 tagA AAA22844.1 CP011101.1_cds_BsLM_3591_3587 100 256 0 tagB AAA22845.1 CP011101.1_cds_BsLM_3592_3588 100 381 0 tagD AAA22843.1 CP011101.1_cds_BsLM_3590_3586 100 129 2.00E-92 tagF NP_391453.1 CP011101.1_cds_BsLM_3588_3584 99.6 746 0 B_subtilis_subsp_subtilis_PS832 tarA CAC86111.1 CP010053.1_cds_QX56_19365_3743 59.53 257 4.00E-110 tarB CAC86110.1 CP010053.1_cds_QX56_19370_3744 49.21 378 3.00E-132 tarD CAC86112.1 CP010053.1_cds_QX56_19360_3742 76.74 129 1.00E-71 tarF CAC86113.1 CP010053.1_cds_QX56_19350_3740 63.43 391 2.00E-178 tagA AAA22844.1 CP010053.1_cds_QX56_19365_3743 100 256 0 tagB AAA22845.1 CP010053.1_cds_QX56_19370_3744 100 381 0 tagD AAA22843.1 CP010053.1_cds_QX56_19360_3742 100 129 2.00E-92 tagF NP_391453.1 CP010053.1_cds_QX56_19350_3740 100 746 0 B_subtilis_subsp_subtilis_PY79 tarA CAC86111.1 CP006881.1_cds_U712_17945_3567 59.53 257 4.00E-110 tarB CAC86110.1 CP006881.1_cds_U712_17950_3568 49.86 367 4.00E-131 tarD CAC86112.1 CP006881.1_cds_U712_17940_3566 76.74 129 1.00E-71 tarF CAC86113.1 CP006881.1_cds_U712_17930_3564 63.43 391 2.00E-178 tagA AAA22844.1 CP006881.1_cds_U712_17945_3567 100 256 0 tagB AAA22845.1 CP006881.1_cds_U712_17950_3568 100 370 0 tagD AAA22843.1 CP006881.1_cds_U712_17940_3566 100 129 2.00E-92 tagF NP_391453.1 CP006881.1_cds_U712_17930_3564 100 746 0 B_subtilis_subsp_subtilis_QB928 tarA CAC86111.1 CP003783.1_cds_B657_35750_3495 59.53 257 4.00E-110 tarB CAC86110.1 CP003783.1_cds_B657_35760_3496 49.21 378 4.00E-132 tarD CAC86112.1 CP003783.1_cds_B657_35740_3494 76.74 129 1.00E-71 tarF CAC86113.1 CP003783.1_cds_B657_35720_3492 63.43 391 3.00E-178 tagA AAA22844.1 CP003783.1_cds_B657_35750_3495 100 256 0 tagB AAA22845.1 CP003783.1_cds_B657_35760_3496 100 381 0 tagD AAA22843.1 CP003783.1_cds_B657_35740_3494 100 129 2.00E-92 tagF NP_391453.1 CP003783.1_cds_B657_35720_3492 100 746 0 B_subtilis_subsp_subtilis_SZMC_6179J tarA CAC86111.1 CP015004.1_cds_A3772_19240_3731 59.53 257 4.00E-110 tarB CAC86110.1 CP015004.1_cds_A3772_19245_3732 49.21 378 3.00E-132 tarD CAC86112.1 CP015004.1_cds_A3772_19235_3730 76.74 129 1.00E-71 tarF CAC86113.1 CP015004.1_cds_A3772_19225_3728 63.43 391 2.00E-178 tagA AAA22844.1 CP015004.1_cds_A3772_19240_3731 100 256 0 tagB AAA22845.1 CP015004.1_cds_A3772_19245_3732 100 381 0 tagD AAA22843.1 CP015004.1_cds_A3772_19235_3730 100 129 2.00E-92 tagF NP_391453.1 CP015004.1_cds_A3772_19225_3728 100 746 0 B_subtilis_subsp_subtilis_BSn5 tarA CAC86111.1 CP002468.1_cds_BSn5_08790_1744 59.53 257 4.00E-110 tarB CAC86110.1 CP002468.1_cds_BSn5_08795_1745 49.86 367 4.00E-131 tarD CAC86112.1 CP002468.1_cds_BSn5_08785_1743 76.74 129 1.00E-71 tarF CAC86113.1 CP002468.1_cds_BSn5_08775_1741 62.76 392 3.00E-178 tagA AAA22844.1 CP002468.1_cds_BSn5_08790_1744 100 256 0 tagB AAA22845.1 CP002468.1_cds_BSn5_08795_1745 100 370 0 tagD AAA22843.1 CP002468.1_cds_BSn5_08785_1743 100 129 2.00E-92 tagF NP_391453.1 CP002468.1_cds_BSn5_08775_1741 99.46 746 0 B_subtilis_subsp_subtilis_SG6 tarA CAC86111.1 CP009796.1_cds_OB04_03583_3464 59.53 257 2.00E-110 tarB CAC86110.1 CP009796.1_cds_OB04_03584_3465 49.21 378 6.00E-132 tarD CAC86112.1 CP009796.1_cds_OB04_03582_3463 76.74 129 1.00E-71 tarF CAC86113.1 CP009796.1_cds_OB04_03580_3461 62.76 392 1.00E-178 tagA AAA22844.1 CP009796.1_cds_OB04_03583_3464 99.22 256 0 tagB AAA22845.1 CP009796.1_cds_OB04_03584_3465 99.74 381 0 tagD AAA22843.1 CP009796.1_cds_OB04_03582_3463 100 129 2.00E-92 tagF NP_391453.1 CP009796.1_cds_OB04_03580_3461 99.58 721 0 B_subtilis_subsp_subtilis_VV2 tarA CAC86111.1 CP017676.1_cds_BKN48_07235_1416 65.37 257 1.00E-127 tarB CAC86110.1 CP017676.1_cds_BKN48_07240_1417 65.88 381 1.00E-171 tarD CAC86112.1 CP017676.1_cds_BKN48_07220_1413 66.67 129 9.00E-64 tarF CAC86113.1 CP017676.1_cds_BKN48_07260_1421 30.63 395 2.00E-39 tagA AAA22844.1 CP017676.1_cds_BKN48_07235_1416 57.98 257 3.00E-105 tagB AAA22845.1 CP017676.1_cds_BKN48_07240_1417 49.59 367 1.00E-123 tagD AAA22843.1 CP017676.1_cds_BKN48_07220_1413 68.99 129 2.00E-66 tagF NP_391453.1 CP017676.1_cds_BKN48_07225_1414 27.34 256 7.00E-11 S_aureus_subsp_aureus_DSM_20231T tarA CAC86111.1 CP011526.1_cds_AA076_03120_594 34.67 225 1.00E-40 tarB CAC86110.1 CP011526.1_cds_AA076_03135_597 29.55 379 6.00E-44 tarD CAC86112.1 CP011526.1_cds_AA076_03145_599 64.29 126 4.00E-57 tarF CAC86113.1 CP011526.1_cds_AA076_01050_205 47.09 378 2.00E-120 tagA AAA22844.1 CP011526.1_cds_AA076_03120_594 32.89 228 2.00E-40 tagB AAA22845.1 CP011526.1_cds_AA076_03135_597 31.93 357 2.00E-48 tagD AAA22843.1 CP011526.1_cds_AA076_03145_599 69.53 128 1.00E-64 tagF NP_391453.1 CP011526.1_cds_AA076_01050_205 48.7 386 2.00E-131 Supplementary Table 3. Genomic COG distribution of B. subtilis subsp. subtilis KCTC 3135 T and related strains B . subtilis subsp. subtilis KCTC 3135T B . subtilis subsp. spizizenii TU-B-10T B . subtilis subsp. inaquosorum KCTC 13429T B . tequilensis KCTC 13622T B . halotolerans ATCC 25096T B . mojavensis RO-H-1T B . atrophaeus NRRL NRS 213T B . nakamurai NRRL B-41091T B . licheniformis DSM 13T B . pumilus ATCC 7061T B . gobiensis FJAT-4402T B . coagulans ATCC 7050T B . acidiproducens DSM 23148T B . ndiopicus FF3T D Cell cycle control, cell division, chromosome partitioning 35 38 36 34 35 36 36 37 38 36 36 33 34 30 M Cell wall/ membrane/ envelope biogenesis 209 213 208 211 201 210 211 188 187 182 210 127 134 123 N Cell motility 41 40 43 41 43 41 43 41 40 43 40 40 42 44 O Post-translational modification, protein turnover, and chaperones 109 108 107 109 105 111 100 106 115 97 106 101 91 83 T Signal transduction mechanisms 139 149 141 131 135 142 135 130 143 129 139 97 94 189 U Intracellular trafficking, secretion, and vesicular transport 36 31 39 34 38 37 37 35 32 31 35 39 36 31 V Defense mechanisms 65 65 65 51 61 67 58 67 82 58 79 41 52 75 W Extracelluar structures 00 000000003010 Y Nuclear structure 00 000000000000 Z Cytoskeleton 11 000000003000 A RNA processing and modification 0 0 0 0 0 0 0 2 0 0 0 0 0 0 B Chromatin structure and dynamics 1 1 1 2 1 1 1 2 1 1 1 1 2 1 J Translation, ribosomal structure and biogenesis 160 166 164 161 163 176 162 159 168 171 170 148 150 167 K Transcription 288 300 312 267 274 283 282 257 319 296 359 172 185 268 L Replication, recombinaiton and repair 146 141 145 141 136 145 147 146 146 145 160 339 193 157 C Energy production and conversion 175 174 179 172 170 182 168 156 175 157 180 134 139 118 E Amino acid transport and metabolism 301 309 316 294 296 315 303 282 311 306 362 214 258 296 F Nucleotide transport and metabolism 87 79 80 84 85 81 80 78 84 80 97 68 78 96 G Carbohydrate transport and metabolism 271 279 290 232 265 280 250 191 305 226 312 163 229 139 H Coenzyme transport and metabolism 103 104 105 99 104 109 105 99 103 97 105 80 85 94 I Lipid transport and metabolism 96 101 112 100 103 101 103 96 85 94 68 74 75 80 P Inorganic ion transport and metabolism 207 208 210 198 205 209 189 180 221 162 279 146 148 201 Q Secondary metabolites biosynthesis, transport and catabolism 60 56 83 67 63 53 72 56 55 60 48 32 33 41 R General function prediction only 0 0 0 0 0 0 0 0 0 0 0 0 0 0 S Function unknown 1245 1251 1228 1193 1230 1173 1218 1113 1286 1166 1407 946 973 1360 X Not matched to database 00 000000000000 Total 3775 3814 3864 3621 3713 3752 3700 3421 3896 3537 4199 2995 3032 3593 Supplementary Table 4. Putative horizonally transferred genes of B . subtilis subsp. subtilis KCTC 3135T Query Length Product Hits Self Close Distal COG Match ANX05678.1 194 L,D-transpeptidase 199 4.77 13.5 69.04 M 1385 (Bacillales) ANX05700.1 198 hypothetical protein AS891_00205 368 8.5 21.79 118.41 S 1385 (Bacillales) ANX05708.1 659 alpha-amylase 135 2.7 30.82 33.61 G 1385 (Bacillales) ANX05726.1 231 zinc ABC transporter ATP-binding protein 311 2 32.69 151.3 P 1385 (Bacillales) ANX05740.1 127 hypothetical protein AS891_00415 309 7.45 25.48 51.96 S 1385 (Bacillales) ANX05758.1 312 hypothetical protein AS891_00510 345 3.91 16.89 60.78 S 1385 (Bacillales) ANX05764.1 455 glucarate transporter 226 3.96 32.16 129.45 G 1385 (Bacillales) ANX05776.1 249 glucosamine-6-phosphate deaminase 310 3.98 19.35 143.26 G 1385 (Bacillales) ANX05781.1 446 hydrolase 171 5.89 32.28 28.16 S 1385 (Bacillales) ANX05786.1 296 carboxylesterase 280 6.56 29.5 59.48 S 1385 (Bacillales) ANX05788.1 313 peptidase 195 4.94 29.33 37.43 O 1385 (Bacillales) ANX05790.1 275 AraC family transcriptional regulator 271 2.73 34.88 120.38 K 1385 (Bacillales) ANX05791.1 306 multidrug transporter 268 2.98 22.14 123.81 E, G 1121307 (Clostridium cylindrosporum DSM 605) ANX05792.1 732 hypothetical protein AS891_00685 158 3.96 29.16 33.5 M 1385 (Bacillales) ANX05813.1 141 thioredoxin 289 3.93 22.84 40.39 O 33936 (Aeribacillus pallidus) ANX05816.1 239 multidrug ABC transporter ATP-binding protein 396 2.99 22.89 106.16 V 1313 (Streptococcus pneumoniae) ANX05818.1 410 sporulation killing factor system radical SAM maturase 355 2.79 11.1 41.69 S 186817 (Bacillaceae) ANX05846.1 529 AraC family transcriptional regulator 195 2 33.9 41.09 K 1385 (Bacillales) ANX05850.1 250 hypothetical protein AS891_01005 211 4.23 31.75 52.27 S 1385 (Bacillales) ANX05871.1 37 50S ribosomal protein L36 395 1.97 12.48 353.93 J 407036 (Sediminibacillus albus) ANX05872.1 72 translation initiation factor IF-1 370 1.99 32.48 304.54 J 1385 (Bacillales) ANX06020.1 146 hypothetical protein AS891_02090 179 4.67 23.01 25.81 S 1385 (Bacillales) ANX06032.1 311 catabolite control protein B 189 3.76 34.71 52.07 K 1385 (Bacillales) ANX06035.1 451 MFS transporter 238 4.23 26.41 80.4 G 1385 (Bacillales) ANX06036.1 299 hypothetical protein AS891_02170 294 5.94 34.2 47.41 S 1385 (Bacillales) ANX06039.1 138 transcriptional regulator 266 5.24 27.91 71.02 K 79264 (Paenibacillus ehimensis) ANX06041.1 458 tetracycline resistance MFS efflux pump 111 3.58 20.48 54.36 G 1389960 (Exiguobacterium sp. S3-2) ANX06043.1 118 hypothetical protein AS891_02205 299 2.99 13.08 137.28 S 84406 (Virgibacillus) ANX06048.1 220 hypothetical protein AS891_02230 218 3.56 32.35 44.81 S 1385 (Bacillales) ANX06056.1 218 multidrug ABC transporter ATP-binding protein 290 1.97 21.52 120.83 P 694055 (Ornithinibacillus contaminans) ANX06059.1 251 hypothetical protein AS891_02285 74 1.17 4.97 17.39 150247 (Anoxybacillus) ANX06064.1 435 MFS transporter 283 5.96 32.66 83.87 G 1385 (Bacillales) ANX06068.1 125 ArsR family transcriptional regulator 216 4 34.39 102.6 K 1385 (Bacillales) ANX06078.1 66 hypothetical protein AS891_02380 117 3.61 23.77 39.38 1385 (Bacillales) ANX06107.1 319 YydG family peptide radical SAM peptide maturase 311 3 7.05 47.96 S 1385 (Bacillales) ANX06108.1 252 peptidase 157 3.98 7.5 23.17 1385 (Bacillales) ANX06109.1 209 peptide ABC transporter ATP-binding protein 258 3.93 13.18 59.29 P 1385 (Bacillales) ANX06110.1 240 peptide ABC transporter permease 129 1.99 9.04 49.86 1385 (Bacillales) ANX06113.1 76 PTS sugar transporter subunit IIA 115 5.16 25.13 38.3 G 1385 (Bacillales) ANX06122.1 343 general stress protein 218 3.5 30.61 54.32 M 1385 (Bacillales) ANX06126.1 356 oxidoreductase 287 1.99 21.56 110.52 S 1385 (Bacillales) ANX06128.1 337 quercetin 2,3-dioxygenase 324 2.98 27.58 64.53 S 1385 (Bacillales) ANX06130.1 151 hypothetical protein AS891_02675 251 3.84 31.6 61.95 S 1385 (Bacillales) ANX06132.1 410 hypothetical protein AS891_02685 248 6.94 16.54 42.69 M 1962118 (Mycobacterium abscessus subsp. massiliense) ANX06133.1 399 MFS transporter 338 2.96 5.5 34.59 G 654421 (Anoxybacillus sp. SK3-4) ANX06134.1 747 asparagine synthetase B 390 2 4.64 65.94 E 654421 (Anoxybacillus sp. SK3-4) ANX06137.1 244 hypothetical protein AS891_02710 388 2 5.02 59.78 S 654421 (Anoxybacillus sp. SK3-4) ANX06138.1 330 hypothetical protein AS891_02715 354 2 3.77 41.12 S 1406 (Paenibacillus polymyxa) ANX06140.1 445 aldehyde dehydrogenase 287 2.97 29.81 115.49 C 1385 (Bacillales) ANX06149.1 251 DeoR family transcriptional regulator 251 4.25 32.49 88.47 K 1385 (Bacillales) ANX06165.1 321 iron(3+)-hydroxamate-binding protein yxeB 158 5.96 29.68 53.5 P 1385 (Bacillales) ANX06167.1 117 hypothetical protein AS891_02860 127 5.21 25.65 21.31 1385 (Bacillales) ANX06176.1 264 ABC transporter substrate-binding protein 229 2.98 31.24 82.31 E 1385 (Bacillales) ANX06181.1 370 ethanolamine utilization protein EutH 290 6.9 28.84 126.08 E 1385 (Bacillales) ANX06182.1 275 hypothetical protein AS891_02935 368 3.04 30.6 93.33 S 91061 (Bacilli) ANX06201.1 148 universal stress protein 338 2.95 27.23 103.05 T 1385 (Bacillales) ANX06209.1 161 hypothetical protein AS891_03080 188 10.62 34.95 27.63 S 311244 (Paenibacillus assamensis) ANX06213.1 382 esterase 188 4 26.21 64.47 S 1385 (Bacillales) ANX06233.1 162 hypothetical protein AS891_03200 319 3.69 31.23 77.51 S 1385 (Bacillales) ANX06236.1 218 DNA-binding response regulator 287 2.98 29.49 118.72 T 1385 (Bacillales) ANX06237.1 406 histidine kinase 176 5.07 20.8 27.14 T 1385 (Bacillales) ANX06240.1 168 hypothetical protein AS891_03240 237 2 8.8 100.98 S 1413210 (Orenia metallireducens) ANX06242.1 213 hypothetical protein AS891_03250 185 4.63 24.84 21.55 1385 (Bacillales) ANX06249.1 560 peptidase 294 15.47 26.2 22.89 O 1313 (Streptococcus pneumoniae) ANX06250.1 450 malate permease 219 4.11 27.1 100.74 C 1385 (Bacillales) ANX06286.1 286 general stress protein A 122 5.61 29.76 33.94 M 1385 (Bacillales) ANX06302.1 385 peptidase M75 278 2 29.01 70.2 P 91061 (Bacilli) ANX06339.1 389 UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase 328 2 32.82 147.3 E 1385 (Bacillales) ANX06340.1 289 spore coat protein 246 2 22.25 23.22 M 1385 (Bacillales) ANX06341.1 373 spore coat protein 309 2.72 32.22 81.28 M 1385 (Bacillales) ANX06342.1 240 spore coat protein 261 1.99 34.02 55.95 M 1385 (Bacillales) ANX06343.1 339 spore coat protein 236 3.61 21.86 28.36 M 1385 (Bacillales) ANX06354.1 204 bacilysin biosynthesis protein BacA 278 3.98 28.97 47.31 E 1385 (Bacillales) ANX06355.1 235 cupin 98 4.59 27.28 15.28 S 1385 (Bacillales) ANX06356.1 472 carboxylate--amine ligase 227 2.98 27.06 29.64 I 1385 (Bacillales) ANX06369.1 156 transcriptional regulator 305 4.68 24.42 81.34 K 1500254 (Aneurinibacillus soli) ANX06370.1 466 MFS transporter 264 4.28 24.25 84.3 V 33942 (Brevibacillus thermoruber) ANX06386.1 426 peptidase M16 291 3.82 17.2 28.58 O 1385 (Bacillales) ANX06389.1 239 multidrug ABC transporter ATP-binding protein 304 2 21.37 68.22 S 1385 (Bacillales) ANX06391.1 448 radical SAM/SPASM domain-containing protein 300 6.71 17.07 42.97 L 1385 (Bacillales) ANX06464.1 105 urease subunit gamma 380 4.98 23.52 235.11 E 1385 (Bacillales) ANX06465.1 124 urease subunit beta 380 2 24.52 165.24 E 1385 (Bacillales) ANX06477.1 140 hypothetical protein AS891_04455 140 5.47 23.48 18.18 S 1385 (Bacillales) ANX06479.1 116 nitrogen regulatory PII 359 3 33.24 163.4 E 1385 (Bacillales) ANX06481.1 153 hypothetical protein AS891_04475 314 7.42 24.82 64.78 1385 (Bacillales) ANX06494.1 114 hypothetical protein AS891_04550 167 5.72 14.64 30.93 M 1385 (Bacillales) ANX06495.1 102 sortase 252 14.41 31.42 88.26 M 1313 (Streptococcus pneumoniae) ANX06512.1 154 hypothetical protein AS891_04640 231 1.96 11.59 42.94 S 44249 (Paenibacillus) ANX06556.1 673 glycosyl transferase family 1 224 3.99 22.42 17.45 M 1462524 (Paraliobacillus sp. PM-2) ANX06561.1 446 glycosyl transferase family A 183 3.68 11.18 29.23 M 1313 (Streptococcus pneumoniae) ANX06562.1 900 glycosyl transferase family A 194 2.81 13.28 30.53 M 69966 (Macrococcus caseolyticus) ANX06583.1 98 competence protein ComF 142 3.66 28.74 25.68 1385 (Bacillales) ANX06609.1 189 TetR family transcriptional regulator 212 2.93 31.4 38.22 K 1385 (Bacillales) ANX06621.1 169 MarR family transcriptional regulator 169 2.33 31.25 44.21 K 1385 (Bacillales) ANX06622.1 248 tRNA-dependent cyclodipeptide synthase 292 3.92 19.39 54.93 1385 (Bacillales) ANX06623.1 405 cytochrome 333 3.96 21.45 125.04 Q 1385 (Bacillales) ANX06624.1 157 MarR family transcriptional regulator 182 2.99 25.1 32.69 K 1385 (Bacillales) ANX06634.1 221 pectate lyase 265 6.32 18 89.17 G 86667 (Jeotgalibacillus marinus) ANX06666.1 316 LacI family transcriptional regulator 217 5.98 22.63 99.31 K 1385 (Bacillales) ANX06667.1 589 alpha-glycosidase 174 11.79 32.53 67.05 G 1313 (Streptococcus pneumoniae) ANX06668.1 417 sugar ABC transporter substrate-binding protein 179 2.99 24.99 82.12 G 1385 (Bacillales) ANX06669.1 435 sugar ABC transporter permease 193 4.24 24.86 98.85 P 1385 (Bacillales) ANX06670.1 294 maltodextrin utilization protein yvdJ 164 4.98 19.31 20.01 S 1385 (Bacillales) ANX06676.1 447 FAD-binding protein 148 2 28.78 51.72 C 255247 (Fictibacillus arsenicus) ANX06678.1 106 molecular chaperone 366 2.95 31.1 144.57 P 1385 (Bacillales) ANX06684.1 451 penicillin-binding protein 223 5.79 31.23 58.6 V 1385 (Bacillales) ANX06686.1 161 PadR family transcriptional regulator 237 1.99 31.44 159.33 Q 1385 (Bacillales) ANX06698.1 344 glycosyltransferase 168 2.97 34.55 24.28 M 1385 (Bacillales) ANX06703.1 322 pyruvyl transferase 233 3.94 29.61 65.88 M 1385 (Bacillales) ANX06722.1 344 glycosylase 268 4.19 33.12 55.86 G 586416 (Terribacillus aidingensis) ANX06728.1 464 MFS transporter 266 2.95 29 103.46 G 1385 (Bacillales) ANX06738.1 232 hypothetical protein AS891_05830 208 2.98 30.26 22.77 1385 (Bacillales) ANX06747.1 207 immunity protein SdpI 183 3.49 21.53 25.09 S 2018042 (Halalkalibacillus sp. B3227) ANX06749.1 323 sporulation-delaying protein SdpB 293 2.5 13.77 50.45 2055160 (Kyrpidia sp. EA-1) ANX06750.1 158 sporulation protein 117 2.39 9.81 15.07 283734 (Staphylococcus pseudintermedius) ANX06756.1 566 chemotaxis protein 247 2.64 33.93 36.69 D 1385 (Bacillales) ANX06758.1 139 transcriptional regulator 245 4.52 34.42 47.87 K 1385 (Bacillales) ANX06759.1 135 transcriptional regulator 247 5.95 34.18 55.05 K 1385 (Bacillales) ANX06766.1 190 TetR family transcriptional regulator 232 2 28.5 50.37 K 1385 (Bacillales) ANX06769.1 631 hypothetical protein AS891_05995 232 2.62 32.37 32.41 S 1385 (Bacillales) ANX06783.1 161 stress protein 133 3.9 30.46 26.21 1385 (Bacillales) ANX06795.1 397 transporter 150 1.99 25.55 18.01 V 1385 (Bacillales) ANX06797.1 409 peptide ABC transporter permease 246 6.99 34.34 61.08 V 1385 (Bacillales) ANX06802.1 79 hypothetical protein AS891_06170 113 3.67 30.25 15.24 1385 (Bacillales) ANX06812.1 225 protein LiaH 257 2 35.17 55.22 K, T 1385 (Bacillales) ANX06834.1 109 hypothetical protein AS891_06340 316 5.86 32.76 136.47 I 1385 (Bacillales) ANX06836.1 127 tautomerase 276 4.31 18.67 116.3 S 1385 (Bacillales) ANX06852.1 45 hypothetical protein AS891_06450 90 5.7 30.22 18.82 1385 (Bacillales) ANX06869.1 242 GntR family transcriptional regulator 201 16.51 34.81 47.84 K 1385 (Bacillales) ANX06877.1 277 xanthine dehydrogenase subunit C 329 2.96 30.7 47.35 C 1385 (Bacillales) ANX06881.1 114 5-hydroxyisourate hydrolase 320 4.85 21.86 148.22 F 1385 (Bacillales) ANX06914.1 47 hypothetical protein AS891_06780 90 4.8 28.63 20.69 1385 (Bacillales) ANX06927.1 69 protein mbtH 326 2.97 17.78 200.91 S 91061 (Bacilli) ANX06948.1 299 competence protein ComQ 196 6.39 31.62 19.09 H 1313 (Streptococcus pneumoniae) ANX07002.1 485 rhamnulokinase 192 2.98 22.23 85.29 G 1385 (Bacillales) ANX07003.1 104 L-rhamnose mutarotase 254 2.94 34.74 129.45 S 91061 (Bacilli) ANX07008.1 161 cysteine dioxygenase 265 2.99 30.09 40.74 S 1385 (Bacillales) ANX07010.1 282 glycosyl transferase 111 4 20.18 28.38 N, U 1385 (Bacillales) ANX07011.1 87 hypothetical protein AS891_07290 285 1.99 33.55 82.32 S 1385 (Bacillales) ANX07017.1 192 molybdenum cofactor sulfurase 338 2.94 33.95 107.06 S 1385 (Bacillales) ANX07058.1 134 hypothetical protein AS891_07655 166 2.98 34.95 24.02 S 1385 (Bacillales) ANX07119.1 385 transporter 247 2.91 31.26 15.08 U 1385 (Bacillales) ANX07176.1 308 LysR family transcriptional regulator 201 3.75 25.07 52.47 K 1313 (Streptococcus pneumoniae) ANX07177.1 178 acetyltransferase 170 2.99 29.32 59.08 S 1639 (Listeria monocytogenes) ANX07178.1 269 L-cystine-binding protein TcyJ 155 4.11 30.86 50.1 E 1313 (Streptococcus pneumoniae) ANX07179.1 270 L-cystine-binding protein TcyJ 156 4.92 29.95 55.46 E 1639 (Listeria monocytogenes) ANX07180.1 239 cysteine ABC transporter permease 249 3 26.8 97.6 E 1639 (Listeria monocytogenes) ANX07181.1 235 cysteine ABC transporter permease 278 2.93 33.06 97.33 E 1637 (Listeria) ANX07183.1 334 hypothetical protein AS891_08300 193 1.99 34.68 51.16 C 1313 (Streptococcus pneumoniae) ANX07184.1 93 NrdH-redoxin 215 2 33.63 65.3 O 2022552 (Paenibacillus sp. 7884-2) ANX07185.1 442 monooxygenase 174 2 27.53 86.23 C 1313 (Streptococcus pneumoniae) ANX07186.1 230 hypothetical protein AS891_08315 363 3.43 17.87 65.22 H 1118054 (Brevibacillus massiliensis) ANX07187.1 416 hydrolase 111 4.55 31.03 27.88 E 1632858 (Massilibacterium senegalense) ANX07193.1 63 hypothetical protein AS891_08350 155 1.98 29.31 44.35 1385 (Bacillales) ANX07281.1 273 stress protein 229 3.47 35.03 30.77 S 1385 (Bacillales) ANX07400.1 180 methyltransferase 288 6.27 25.92 41.24 Q 1385 (Bacillales) ANX07403.1 96 hypothetical protein AS891_09460 345 5.34 21.05 112.23 86667 (Jeotgalibacillus marinus) ANX07410.1 935 PTS sugar transporter subunit IIA 192 5.98 33.16 57.4 K 1313 (Streptococcus pneumoniae) ANX07411.1 146 PTS fructose transporter subunit IIA 253 4.99 16.24 77.01 G 1385 (Bacillales) ANX07412.1 162 PTS fructose transporter subunit IIB 284 4 18.57 135.96 G 1313 (Streptococcus pneumoniae) ANX07413.1 269 PTS mannose/fructose/sorbose transporter subunit IIC 278 2.99 15.06 139.91 G 1385 (Bacillales) ANX07414.1 275 PTS mannose transporter subunit IID 252 3 11.65 149.57 G 1385 (Bacillales) ANX07418.1 140 transcriptional regulator 303 5.97 12.86 142.05 K 1385 (Bacillales) ANX07425.1 111 hypothetical protein AS891_09575 289 9.87 9.77 99.54 S 1385 (Bacillales) ANX07426.1 118 hypothetical protein AS891_09580 296 9.13 8.46 90.85 S 1385 (Bacillales) ANX07429.1 277 chitosanase 301 3 23.44 71.23 M 1385 (Bacillales) ANX07433.1 176 RNA polymerase subunit sigma-70 314 2.81 10.12 116.49 K 1385 (Bacillales) ANX07441.1 129 hypothetical protein AS891_09665 147 5.82 15.02 34.71 S 1484460 (Psychroserpens jangbogonensis) ANX07446.1 157 ArsR family transcriptional regulator 362 5.9 6.64 147.5 K 558169 (Lentibacillus jeotgali) ANX07459.1 152 spermidine acetyltransferase 231 4.69 31.85 81.71 K 1385 (Bacillales) ANX07472.1 158 hypothetical protein AS891_09820 122 3.32 20.23 35.69 44249 (Paenibacillus) ANX07474.1 185 acetyltransferase 307 2 22.14 119.77 S 2022549 (Paenibacillus sp. 7516) ANX07477.1 231 DNA-binding response regulator 279 1.99 30.03 115.41 T 1926588 (Paenibacillus sp. FSL R7-0337) ANX07478.1 432 histidine kinase 259 4.94 24.29 52.37 T 1926588 (Paenibacillus sp. FSL R7-0337) ANX07482.1 178 hypothetical protein AS891_09875 325 2.58 21.43 140.62 F 161536 (Ornithinibacillus californiensis) ANX07484.1 116 XRE family transcriptional regulator 221 2.81 23.1 49.22 K 2022548 (Virgibacillus sp. 7505) ANX07490.1 284 recombinase RecT 203 2.64 30.12 72.72 L 1926277 (Sediminibacillus massiliensis) ANX07502.1 305 hypothetical protein AS891_09985 187 2.85 30.54 27.22 S 1962118 (Mycobacterium abscessus subsp. massiliense) ANX07504.1 322 phage portal protein 111 2.44 32.31 18.87 1385 (Bacillales) ANX07508.1 120 phage portal protein 84 1.98 30.25 15.98 2 (Bacteria) ANX07513.1 147 phage portal protein 288 2 25.88 100.28 S 1385 (Bacillales) ANX07515.1 149 phage portal protein 264 4.04 29.86 60.08 S 1385 (Bacillales) ANX07520.1 87 phage portal protein 117 2.62 29.57 24.09 S 1385 (Bacillales) ANX07534.1 192 hypothetical protein AS891_10145 279 3.9 16.59 75.16 S 694055 (Ornithinibacillus contaminans) ANX07544.1 500 DNA recombinase 304 5.75 23.16 61.7 L 1313 (Streptococcus pneumoniae) ANX07546.1 240 hypothetical protein AS891_10210 280 9.85 26.79 68.07 1385 (Bacillales) ANX07581.1 57 30S ribosomal protein S21 335 1 33.01 263.7 J 1385 (Bacillales) ANX07770.1 439 NAD-dependent malic enzyme 1 328 3 34.9 128.5 C 1385 (Bacillales) ANX07906.1 339 LacI family transcriptional regulator 230 2 31.23 68.71 K 1385 (Bacillales) ANX07909.1 330 2-keto-3-deoxygluconate permease 249 2.99 33.05 101.89 P 1385 (Bacillales) ANX07911.1 194 xanthine phosphoribosyltransferase 206 2.99 35.13 118.78 F 1385 (Bacillales) ANX07953.1 545 resolvase 194 2 18.45 29.12 L 1313 (Streptococcus pneumoniae) ANX07960.1 296 endonuclease 177 5.91 31.7 39.85 L 1385 (Bacillales) ANX07964.1 165 hypothetical protein AS891_12395 162 2.59 31.17 46.01 225985 (Fictibacillus gelatini) ANX07965.1 192 hypothetical protein AS891_12400 209 5.61 21.99 24.86 S 1962118 (Mycobacterium abscessus subsp. massiliense) ANX07966.1 177 acetyltransferase 325 4.86 17.66 91.1 J 1421 (Lysinibacillus sphaericus) ANX07975.1 705 peptidase C39 188 3.98 8.98 37.13 V 1817674 (Geobacillus sp. 8) ANX07976.1 137 thiol reductase thioredoxin 186 1.98 17.75 28.89 O 1920423 (Paenibacillus sp. FSL H8-0259) ANX07977.1 422 SunS family peptide S-glycosyltransferase 266 2.8 8.87 31.03 M 1817674 (Geobacillus sp. 8) ANX07979.1 88 holin 155 2.94 33.52 30.82 S 1385 (Bacillales) ANX07980.1 70 hypothetical protein AS891_12475 96 3.14 21.55 26.79 S 2025389 (Terribacillus sp. 7520-G) ANX07981.1 367 N-acetylmuramoyl-L-alanine amidase 161 3 21.34 18.48 M 1962118 (Mycobacterium abscessus subsp. massiliense) ANX07984.1 644 hypothetical protein AS891_12495 296 2 4.2 20.19 G 1763509 (Lachnotalea glycerini) ANX07993.1 333 recombinase 155 3.68 27.74 20.64 L 1572712 (Geobacillus virus E3) ANX08013.1 589 hypothetical protein AS891_12645 135 2.99 15.86 27.36 586416 (Terribacillus aidingensis) ANX08020.1 839 hypothetical protein AS891_12680 201 2.73 21.17 28.49 1459 (Sporosarcina globispora) ANX08022.1 108 transcriptional regulator 274 3.08 19.2 66.26 K 1385 (Bacillales) ANX08026.1 410 hypothetical protein AS891_12710 206 2.98 29.42 38.77 1406 (Paenibacillus polymyxa) ANX08028.1 438 hypothetical protein AS891_12720 147 1 1.6 20.95 459472 (Planomicrobium glaciei) ANX08029.1 75 hypothetical protein AS891_12725 161 3.29 22.61 50 130049 (Paenibacillus dendritiformis) ANX08030.1 442 hypothetical protein AS891_12730 203 1.34 7.21 21.63 1385 (Bacillales) ANX08037.1 178 hypothetical protein AS891_12765 114 2.19 6.15 19.58 170674 (Vibrio pacinii) ANX08059.1 251 antirepressor 182 1.83 15.49 34.46 K 1500254 (Aneurinibacillus soli) ANX08065.1 171 hypothetical protein AS891_12905 256 2 20.74 40.12 S 883812 (Geobacillus galactosidasius) ANX08075.1 137 hypothetical protein AS891_12955 107 2.27 20.84 16.35 1313 (Streptococcus pneumoniae) ANX08090.1 378 hypothetical protein AS891_13030 123 2 14.29 15.12 586416 (Terribacillus aidingensis) ANX08091.1 576 single-stranded-DNA-specific exonuclease RecJ 289 2.75 20.33 31.88 L 586416 (Terribacillus aidingensis) ANX08092.1 1305 DNA-dependent DNA polymerase 302 3.56 16.95 40.14 L 586416 (Terribacillus aidingensis) ANX08093.1 219 hypothetical protein AS891_13045 201 5.89 24.57 19.61 S 237069 (Tenuibacillus multivorans) ANX08098.1 172 5'-3'-deoxyribonucleotidase 332 2.89 14.87 104.43 S 1280390 (Gorillibacterium massiliense) ANX08100.1 443 DNA cytosine methyltransferase 316 3.12 12.34 53.8 L 930131 (Oceanobacillus limi) ANX08120.1 173 HNH endonuclease 237 3.97 9.78 39.34 S 1464 (Paenibacillus larvae) ANX08140.1 141 capsule biosynthesis protein CapD 298 2.99 34.39 124.56 M 1385 (Bacillales) ANX08141.1 382 3-phytase 253 5.99 33.11 75.35 I 1385 (Bacillales) ANX08198.1 114 hypothetical protein AS891_13610 361 3.71 22.36 95.07 S 1385 (Bacillales) ANX08201.1 158 hypothetical protein AS891_13625 309 2 29.84 43.73 O 1196324 (Fictibacillus macauensis ZFHKF-1) ANX08214.1 260 hypothetical protein AS891_13695 298 6.58 33.26 56.31 1385 (Bacillales) ANX08219.1 313 transcriptional regulator 233 4.88 28.06 66.72 K 1385 (Bacillales) ANX08223.1 247 acetyltransferase 308 2.97 16.97 59.53 S 2 (Bacteria) ANX08232.1 1201 hypothetical protein AS891_13790 344 2.99 4.28 54.22 553311 (Virgibacillus salinus) ANX08242.1 307 hypothetical protein AS891_13845 284 3.64 4.89 59.77 1347087 (Paucisalibacillus sp. EB02) ANX08246.1 112 transcriptional regulator 255 5.04 32.53 52.99 K 1566279 (Paenibacillus sp. NFR01) ANX08247.1 79 hypothetical protein AS891_13875 376 1.99 8.13 197.19 S 182337 (Erwinia billingiae) ANX08248.1 213 1,4-beta-xylanase 276 2.84 31.71 155.17 G 1385 (Bacillales) ANX08256.1 292 hypothetical protein AS891_13920 332 3.9 24.28 73.82 S 1385 (Bacillales) ANX08257.1 290 LysR family transcriptional regulator 166 3.98 32.9 40.05 K 1385 (Bacillales) ANX08263.1 251 acetyltransferase 289 2 23.5 117.79 S 44252 (Paenibacillus macerans) ANX08268.1 345 pectate lyase 200 5.53 24 73.48 G 1385 (Bacillales) ANX08269.1 225 hypothetical protein AS891_13990 267 5.58 33.25 45.94 S 1385 (Bacillales) ANX08270.1 232 Expansin-YoaJ 224 2.53 29.5 89.58 M 1385 (Bacillales) ANX08276.1 97 hypothetical protein AS891_14025 115 1.98 25.84 16.8 1385 (Bacillales) ANX08278.1 344 hydroxyacid dehydrogenase 189 11.14 25.87 58.61 C 1313 (Streptococcus pneumoniae) ANX08279.1 487 sugar kinase 215 2.98 33.06 41.72 G 1385 (Bacillales) ANX08282.1 256 hypothetical protein AS891_14055 211 5.46 26.65 55.25 558169 (Lentibacillus jeotgali) ANX08285.1 122 Replication termination protein 153 4 28.92 56.19 L 1385 (Bacillales) ANX08292.1 285 transcriptional regulator 187 3.4 27.91 63.1 K 1385 (Bacillales) ANX08319.1 148 hypothetical protein AS891_14250 254 4.65 32.92 59.79 S 1385 (Bacillales) ANX08374.1 279 thymidylate synthase 269 3.79 32.18 94.41 F 1385 (Bacillales) ANX08386.1 156 hypothetical protein AS891_14590 181 2.29 20.16 45.8 334253 (Gracilibacillus orientalis) ANX08392.1 263 hypothetical protein AS891_14620 180 4.28 14.17 31.51 361277 (Terribacillus saccharophilus) ANX08421.1 118 hypothetical protein AS891_14765 144 2 19.2 26.56 1385 (Bacillales) ANX08425.1 4262 polyketide synthase 147 5.3 25.19 24.81 Q 1313 (Streptococcus pneumoniae) ANX08426.1 4532 polyketide synthase 127 14.31 29.89 17.24 Q 1313 (Streptococcus pneumoniae) ANX08428.1 249 enoyl-CoA hydratase 210 3.64 22.21 96.55 M 1385 (Bacillales) ANX08429.1 256 enoyl-CoA hydratase 274 10.55 22.35 99.56 I 1313 (Streptococcus pneumoniae) ANX08430.1 420 3-hydroxy-3-methylglutaryl-ACP synthase 256 3 25.37 142.44 I 1385 (Bacillales) ANX08431.1 415 polyketide beta-ketoacyl:ACP synthase 263 8.7 19.4 97.55 I, Q 1385 (Bacillales) ANX08432.1 82 poly(3-hydroxyalkanoate) depolymerase 230 2.79 13.71 92.1 I, Q 1385 (Bacillales) ANX08433.1 324 acyltransferase 276 2 18.23 89.3 Q 1385 (Bacillales) ANX08434.1 288 malonyl CoA-acyl carrier protein transacylase 132 3.92 24.23 55.66 I 1385 (Bacillales) ANX08435.1 225 polyketide biosynthesis protein 321 2 21.55 101.28 S 1385 (Bacillales) ANX08668.1 97 anti-sigma factor 107 2.59 22.25 17.79 S 1385 (Bacillales) ANX08733.1 200 hypothetical protein AS891_16390 149 6.17 11.24 19.2 M 1462526 (Virgibacillus massiliensis) ANX08734.1 591 hypothetical protein AS891_16395 235 5.89 25.67 71.8 M 1230341 (Salimicrobium jeotgali) ANX08766.1 232 glycosylase 296 2.95 34.27 73.87 G 1385 (Bacillales) ANX08767.1 75 hypothetical protein AS891_16570 229 3.42 31.19 49.33 1385 (Bacillales) ANX08768.1 399 peptigoglycan-binding protein LysM 190 4.71 27.5 34.86 M 1385 (Bacillales) ANX08769.1 248 oxidoreductase 300 3 26.48 168.13 S 561440 (Lysinibacillus sinduriensis) ANX08781.1 143 hypothetical protein AS891_16640 193 2.78 30.24 45.77 S 1385 (Bacillales) ANX08820.1 170 hypothetical protein AS891_16845 243 3.98 29.6 34.61 S 1385 (Bacillales) ANX08824.1 200 thiamine-binding protein 339 3.97 31.86 115.28 S 1385 (Bacillales) ANX08878.1 88 phage portal protein 98 2.88 31.2 18.35 S 1385 (Bacillales) ANX08883.1 148 phage portal protein 265 4.32 29.5 59.71 S 1385 (Bacillales) ANX08884.1 147 phage portal protein 288 2 26.37 101.26 S 1385 (Bacillales) ANX08889.1 118 phage portal protein 87 2 29.39 15.13 1385 (Bacillales) ANX08892.1 275 phage portal protein 112 2.99 34.11 18.03 1385 (Bacillales) ANX08910.1 85 hypothetical protein AS891_17305 322 4.1 25.59 110.44 S 44251 (Paenibacillus durus) ANX08913.1 423 ATPase 354 6.05 33.08 97.47 O 1385 (Bacillales) ANX08934.1 392 UDP-glucosyltransferase 251 3.96 24.41 72.55 C, G 1385 (Bacillales) ANX08941.1 191 hypothetical protein AS891_17460 141 5.78 32.12 20.22 1385 (Bacillales) ANX08945.1 55 hypothetical protein AS891_17480 103 1.95 25.77 29.73 1385 (Bacillales) ANX08949.1 320 protoheme IX farnesyltransferase 286 10.81 33.59 87.87 O 1313 (Streptococcus pneumoniae) ANX08952.1 159 aminoacyl-tRNA deacylase 176 4.96 30.3 73.26 S 1385 (Bacillales) ANX08954.1 168 acetyltransferase 299 3.47 25.15 67.93 J 1385 (Bacillales) ANX08963.1 94 hypothetical protein AS891_17570 62 3 10.08 17.06 1313296 (Paenibacillus uliginis N3/975) ANX08965.1 153 hypothetical protein AS891_17580 174 2.98 31.02 21.32 1460636 (Geomicrobium sp. JCM 19039) ANX08969.1 409 hypothetical protein AS891_17600 308 2 10.79 19.04 1684312 (Exiguobacterium sp. BMC-KP) ANX09055.1 97 hypothetical protein AS891_18055 120 2 31.07 15.78 1385 (Bacillales) ANX09057.1 115 DNA-binding protein 271 2.97 29.94 77.05 K 1386078 (Pseudomonas sp. EGD-AK9) ANX09058.1 178 hypothetical protein AS891_18070 205 3.78 17.63 36.43 459525 (Fictibacillus solisalsi) ANX09059.1 309 hypothetical protein AS891_18075 274 3.59 23.66 55.75 2021314 (Fictibacillus aquaticus) ANX09067.1 371 isomerase 329 3.78 24.86 80.38 M 1385 (Bacillales) ANX09070.1 228 2-phosphosulfolactate phosphatase 365 3.8 31.05 80.97 T 1385 (Bacillales) ANX09076.1 342 oxidoreductase 277 4.48 31.91 91.56 S 1385 (Bacillales) ANX09107.1 441 NTD biosynthesis operon protein NtdA 181 4.52 28.63 39.93 E 1385 (Bacillales) ANX09109.1 350 NTD biosynthesis operon oxidoreductase NtdC 244 4.67 25.99 35.93 S 1385 (Bacillales) ANX09110.1 401 MFS transporter 152 3 24.24 21.91 G 1385 (Bacillales) ANX09111.1 175 transcriptional regulator 181 2.99 30.06 41 K 1385 (Bacillales) ANX09118.1 299 NAD(P)-dependent oxidoreductase 190 5.97 33.34 99.83 S 1385 (Bacillales) ANX09127.1 323 iron-dicitrate ABC transporter substrate-binding protein 152 4.67 30.06 32.52 P 1385 (Bacillales) ANX09201.1 393 aspartate aminotransferase 323 5.6 32.6 120.5 E 1385 (Bacillales) ANX09204.1 331 general stress protein 302 2 22.43 169.96 C 1385 (Bacillales) ANX09257.1 154 MarR family transcriptional regulator 329 2.8 22.33 51.88 K 1385 (Bacillales) ANX09312.1 160 MarR family transcriptional regulator 358 2.98 22.8 73.47 K 1385 (Bacillales) ANX09313.1 518 MFS transporter 215 8.12 29.92 63.44 G 1385 (Bacillales) ANX09315.1 417 MFS transporter 293 2.92 28.97 48.73 G 1313 (Streptococcus pneumoniae) ANX09316.1 205 TetR family transcriptional regulator 338 2.99 32.14 57.5 K 1313 (Streptococcus pneumoniae) ANX09319.1 182 PadR family transcriptional regulator 243 4.91 32.88 78.19 K 1385 (Bacillales) ANX09324.1 392 oxidoreductase 217 7.55 26.76 122.08 S 1313 (Streptococcus pneumoniae) ANX09325.1 313 hypothetical protein AS891_19580 261 2 26.36 142.27 G 1385 (Bacillales) ANX09326.1 314 transcriptional regulator 289 3.37 23.46 55.05 K 1385 (Bacillales) ANX09331.1 527 PTS alpha-glucoside transporter subunit IIBC 191 2.68 29.65 98.03 G 1385 (Bacillales) ANX09332.1 254 RpiR family transcriptional regulator 218 5.98 29.73 59.95 K 1385 (Bacillales) ANX09333.1 449 6-phospho-alpha-glucosidase 201 2 28.73 125.66 G 1385 (Bacillales) ANX09353.1 286 6-phosphogluconate dehydrogenase 255 7.6 28.49 97.46 I 1385 (Bacillales) ANX09374.1 384 spore gernimation protein 260 4.91 19.18 46.92 S 1157490 (Tumebacillus flagellatus) ANX09376.1 358 spore gernimation protein 308 2.91 14.38 39.69 S 278991 (Aneurinibacillus terranovensis) ANX09394.1 478 anion permease 276 2 31.81 137.98 P 1385 (Bacillales) ANX09395.1 420 pectate lyase 244 2.99 32.05 89.97 G 28031 (Lysinibacillus fusiformis) ANX09398.1 315 iron citrate ABC transporter substrate-binding protein 203 3.96 25.62 66.59 P 1385 (Bacillales) ANX09399.1 333 iron-siderophore ABC transporter permease 158 4.83 32.17 48.06 P 1385 (Bacillales) ANX09400.1 333 iron-dicitrate transporter subunit FecD 216 2 32.86 76 P 1385 (Bacillales) ANX09402.1 487 hypothetical protein AS891_19975 351 3 7.68 44.66 S 1385 (Bacillales) ANX09428.1 369 oxidoreductase 323 4.52 28 66.83 C, H 91061 (Bacilli) ANX09429.1 167 MarR family transcriptional regulator 288 2.62 15.07 81.61 K 91061 (Bacilli) ANX09430.1 330 hypothetical protein AS891_20120 306 4.77 22.26 76.2 S 1385 (Bacillales) ANX09433.1 164 hypothetical protein AS891_20140 275 5.36 32.86 42.25 91061 (Bacilli) ANX09437.1 446 alpha-glucosidase/alpha-galactosidase 304 2.69 21.02 111.43 G 1385 (Bacillales) ANX09439.1 318 protein lplB 235 3.59 35.19 117.58 G 1385 (Bacillales) ANX09441.1 857 hypothetical protein AS891_20180 260 3.32 32.24 81.03 1385 (Bacillales) ANX09447.1 220 hypothetical protein AS891_20210 298 3.75 29.67 111.45 S 1385 (Bacillales) ANX09449.1 761 AraC family transcriptional regulator 207 2.97 26.46 42.66 K 1385 (Bacillales) ANX09453.1 368 DNA-binding response regulator 255 4.75 28 43.06 T 1385 (Bacillales) ANX09454.1 577 two-component sensor histidine kinase 235 3.89 32.52 61.35 T 1385 (Bacillales) ANX09457.1 180 acetyltransferase 207 2 29.5 58.94 S 1385 (Bacillales) ANX09461.1 286 NAD(P)-dependent oxidoreductase 361 9.2 21.08 127.7 S 1313 (Streptococcus pneumoniae) ANX09462.1 147 hypothetical protein AS891_20290 351 2.92 17.68 132.38 S 2 (Bacteria) ANX09463.1 194 TetR family transcriptional regulator 352 3.94 25.42 118.03 K 1385 (Bacillales) ANX09470.1 68 hypothetical protein AS891_20335 100 2 25.21 24.93 S 186817 (Bacillaceae) ANX09472.1 382 hypothetical protein AS891_20345 382 2.99 5.35 113.95 S 1443435 (Aneurinibacillus tyrosinisolvens) ANX09473.1 599 ATP-dependent helicase 357 2 6.4 216.99 L 1443435 (Aneurinibacillus tyrosinisolvens) ANX09474.1 879 DNA methyltransferase 365 2 5.58 155.44 L 1443435 (Aneurinibacillus tyrosinisolvens) ANX09476.1 300 hypothetical protein AS891_20365 331 2.61 16.91 50.46 L 1532552 (Anaerobacillus sp. NB2006) ANX09497.1 424 amino acid permease 221 8.9 26.86 54.17 E 1313 (Streptococcus pneumoniae) ANX09531.1 341 hypothetical protein AS891_20645 341 4.43 11.34 92.92 S 1385 (Bacillales) ANX09533.1 254 hypothetical protein AS891_20655 281 3.97 31.79 67.11 S 1385 (Bacillales) ANX09538.1 829 transcriptional regulator 248 3.24 31.23 15.47 K 1385 (Bacillales) ANX09540.1 465 restriction endonuclease 305 1.29 5.67 29.33 V 1571 (Sporosarcina ureae) ANX09541.1 343 restriction endonuclease 353 2.85 10.73 88.43 V 1571 (Sporosarcina ureae) ANX09546.1 392 methylase 364 3 0.99 131.14 L 1637999 (Verrucomicrobia bacterium IMCC26134) ANX09547.1 427 methylase 376 1 1.94 69.79 L 351091 (Oscillibacter valericigenes) ANX09551.1 101 hypothetical protein AS891_20755 156 2.5 18.15 28.62 1221996 (Quasibacillus thermotolerans) ANX09592.1 152 MarR family transcriptional regulator 227 6.67 20.05 44.16 K 1385 (Bacillales) ANX09597.1 114 hypothetical protein AS891_21020 134 9.31 34.41 21.19 1385 (Bacillales) ANX09612.1 270 GntR family transcriptional regulator 244 4.89 31.53 71.27 K 1385 (Bacillales) ANX09614.1 300 carboxylesterase 272 5.26 28.24 57.7 S 47500 (Aneurinibacillus migulanus) ANX09628.1 61 hypothetical protein AS891_21175 118 3.75 32.32 17.42 1765683 (Acidibacillus ferrooxidans) ANX09633.1 109 transcriptional regulator 293 4.07 27.26 125.69 K 1313 (Streptococcus pneumoniae) ANX09634.1 190 hydrolase 259 6.88 20.14 87.03 S 1385 (Bacillales) ANX09635.1 141 hypothetical protein AS891_21215 311 2.99 33.36 120.8 I 1385 (Bacillales) ANX09637.1 287 transporter 261 4.72 22.54 165.5 E, G 1385 (Bacillales) ANX09638.1 219 hypothetical protein AS891_21230 231 8.24 33.82 45.22 1313 (Streptococcus pneumoniae) ANX09640.1 124 hypothetical protein AS891_21240 101 6.9 31.49 20.61 1313 (Streptococcus pneumoniae) ANX09648.1 197 glutamine amidotransferase 267 1.96 8.02 101.92 S 621109 (Virgibacillus subterraneus) ANX09660.1 266 hypothetical protein AS891_21345 223 1 5.9 32.88 483937 (Paenibacillus riograndensis) ANX09663.1 329 endopeptidase 215 4.9 28.24 60.4 M 459525 (Fictibacillus solisalsi) ANX09665.1 108 hypothetical protein AS891_21370 87 2.96 12.56 20.76 S 1460649 (Geomicrobium sp. JCM 19055) ANX09666.1 797 hypothetical protein AS891_21375 122 7.21 22.7 30.11 S 459525 (Fictibacillus solisalsi) ANX09673.1 352 DNA relaxase NicK 187 4.78 19.51 59.12 K 459525 (Fictibacillus solisalsi) ANX09674.1 480 cell division protein FtsK 164 3.87 18.76 60.6 D 459525 (Fictibacillus solisalsi) ANX09675.1 126 hypothetical protein AS891_21420 200 3.83 15.49 42.34 S 746691 (Oceanobacillus kimchii) ANX09677.1 64 ICEBs1 excisionase 199 1.96 10.46 71.44 1460649 (Geomicrobium sp. JCM 19055) ANX09678.1 127 transcriptional regulator 239 2.98 23.78 53.68 K 746691 (Oceanobacillus kimchii) ANX09679.1 169 peptidase 158 2.45 16.64 22.82 E 2751 (Carnobacterium maltaromaticum) ANX09680.1 368 integrase 225 2.55 15.61 56.7 L 746691 (Oceanobacillus kimchii) ANX09684.1 147 MarR family transcriptional regulator 398 1.99 8.39 140.71 K 2015052 (Bacillaceae bacterium SAOS 7) ANX09685.1 146 EVE domain-containing protein 321 2.81 18.72 126.11 S 2015203 (Bacillaceae bacterium SAS-127) ANX09723.1 150 hypothetical protein AS891_21715 261 6.96 33.48 93.71 S 91061 (Bacilli) ANX09729.1 149 DNA mismatch repair protein MutT 224 6.3 33.43 37.35 L 1385 (Bacillales) ANX09734.1 283 hypothetical protein AS891_21770 217 2.62 35.2 28.12 T 1385 (Bacillales) ANX09776.1 104 transcriptional regulator 227 1.99 34.92 82.82 K 1385 (Bacillales) ANX09797.1 281 alpha/beta hydrolase 292 2 16.36 82.98 S 1385 (Bacillales) ANX09798.1 75 hypothetical protein AS891_22110 194 5.21 20.92 82.8 S 1385 (Bacillales) ANX09799.1 473 phenolic acid decarboxylase 139 2 25.97 77.49 H 1385 (Bacillales) ANX09810.1 408 MFS transporter 282 8.31 18.62 33.36 G 1385 (Bacillales) ANX09815.1 93 transcriptional regulator 280 8.51 20.51 116.86 K 1385 (Bacillales) ANX09842.1 271 sugar ABC transporter permease 201 5.62 26.75 122.54 P 1385 (Bacillales) ANX09843.1 95 hypothetical protein AS891_05555 245 7.9 21.76 94.45 S 1385 (Bacillales) ANX09875.1 240 transcriptional regulator 293 7.85 30.03 153.68 K 1385 (Bacillales) ANX09878.1 326 oxidoreductase 289 7.87 14.82 170.17 C 1385 (Bacillales) ANX09879.1 265 glutamate racemase 312 8.38 22.5 128.75 M 379896 (Paenibacillus fonticola) ANX09880.1 75 transcriptional regulator 233 3.97 28.9 78.94 K 55079 (Aneurinibacillus) ANX09898.1 196 hypothetical protein AS891_12335 255 4.01 34.62 68.17 M 1385 (Bacillales) ANX09934.1 497 hypothetical protein AS891_18355 211 12.59 29.03 69.76 C, H 1313 (Streptococcus pneumoniae) ANX09960.1 292 TetR family transcriptional regulator 232 13.9 34.58 33.26 K 1385 (Bacillales) Supplementary Table 5. Putative horizonally transferred genes of B . subtilis subsp. spizizenii TU-B-10T Query Length Product Hits Self Close Distal COG Match AEP84913.1 37 ribosomal protein L36 395 1.97 12.48 353.93 J 407036 (Sediminibacillus albus) AEP84931.1 394 site-specific recombinase, phage integrase family protein 300 2 21.89 62.01 L 1385 (Bacillales) AEP84932.1 177 conserved hypothetical protein 153 3.81 34.68 19.93 S 1385 (Bacillales) AEP84935.1 77 helix-turn-helix, putative 88 1.98 12.39 27.1 S 1385 (Bacillales) AEP84936.1 177 bacteriophage-related protein 173 2 11.22 37.08 1385 (Bacillales) AEP84939.1 657 conserved hypothetical protein 281 1.99 14.98 82.31 1385 (Bacillales) AEP84940.1 252 phage recombination protein Bet 242 1.92 5.15 75.71 L 1385 (Bacillales) AEP84941.1 190 conserved hypothetical protein 239 1.99 23.16 120.88 S 1385 (Bacillales) AEP84952.1 348 oxidoreductase, FAD/FMN-binding protein 296 1 34.88 149.79 C 1385 (Bacillales) AEP84953.1 177 glyoxalase family protein 289 1 3.34 116.47 S 1385 (Bacillales) AEP84960.1 173 DNA methylase 293 1.97 22.45 108.44 K 1385 (Bacillales) AEP84961.1 339 modification methylase Bsu 319 2 15.41 72.42 L 1385 (Bacillales) AEP84962.1 223 conserved hypothetical protein 195 1.23 10.85 27.44 1385 (Bacillales) AEP84963.1 292 conserved hypothetical protein 208 1.91 23.19 45.07 S 1385 (Bacillales) AEP84966.1 305 phage Mu protein F like protein 182 2.81 31.2 27.35 S 1385 (Bacillales) AEP84968.1 393 conserved hypothetical protein 127 2.14 27.58 25.82 1385 (Bacillales) AEP84969.1 311 conserved hypothetical protein 151 2 36.2 22.59 S 1385 (Bacillales) AEP84977.1 147 conserved hypothetical protein 288 1.99 26.52 103.89 S 1385 (Bacillales) AEP84978.1 149 conserved hypothetical protein 266 4.01 30.1 58.77 S 1385 (Bacillales) AEP84983.1 88 conserved hypothetical protein 105 2.65 31.12 19.98 S 1385 (Bacillales) AEP84986.1 192 conserved hypothetical protein 211 5.43 35.83 32.87 S 1385 (Bacillales) AEP84992.1 72 putative holin 161 3.75 35.35 45.46 S 1385 (Bacillales) AEP84999.1 351 conserved hypothetical protein 388 1 0.52 112.83 1385 (Bacillales) AEP85000.1 394 site-specific recombinase, phage integrase family protein 300 2 21.89 62.01 L 1385 (Bacillales) AEP85001.1 177 conserved hypothetical protein 153 3.81 34.68 19.93 S 1385 (Bacillales) AEP85004.1 77 helix-turn-helix, putative 88 1.98 12.39 27.1 S 1385 (Bacillales) AEP85005.1 177 bacteriophage-related protein 173 2 11.22 37.08 1385 (Bacillales) AEP85008.1 657 conserved hypothetical protein 281 1.99 14.98 82.31 1385 (Bacillales) AEP85009.1 252 phage recombination protein Bet 242 1.92 5.15 75.71 L 1385 (Bacillales) AEP85010.1 190 conserved hypothetical protein 240 2.99 23.16 120.88 S 1385 (Bacillales) AEP85021.1 348 oxidoreductase, FAD/FMN-binding protein 296 1 34.88 149.79 C 1385 (Bacillales) AEP85022.1 177 glyoxalase family protein 289 1 3.34 116.47 S 1385 (Bacillales) AEP85029.1 173 DNA methylase 293 1.97 22.45 108.44 K 1385 (Bacillales) AEP85030.1 339 modification methylase Bsu 319 2 15.41 72.42 L 1385 (Bacillales) AEP85031.1 223 conserved hypothetical protein 196 2.23 10.85 27.44 1385 (Bacillales) AEP85032.1 292 conserved hypothetical protein 208 1.91 23.19 45.07 S 1385 (Bacillales) AEP85035.1 305 phage Mu protein F like protein 182 2.81 31.2 27.35 S 1385 (Bacillales) AEP85037.1 393 conserved hypothetical protein 127 2.14 27.58 25.82 1385 (Bacillales) AEP85038.1 311 conserved hypothetical protein 151 2 36.2 22.59 S 1385 (Bacillales) AEP85046.1 147 conserved hypothetical protein 288 1.99 26.52 103.89 S 1385 (Bacillales) AEP85047.1 149 conserved hypothetical protein 266 4.01 30.1 58.77 S 1385 (Bacillales) AEP85052.1 88 conserved hypothetical protein 105 2.65 31.12 19.98 S 1385 (Bacillales) AEP85055.1 192 conserved hypothetical protein 211 5.43 35.83 32.87 S 1385 (Bacillales) AEP85061.1 72 putative holin 162 4.75 35.35 45.46 S 1385 (Bacillales) AEP85068.1 351 conserved hypothetical protein 388 1 0.52 112.83 1385 (Bacillales) AEP85069.1 407 transporter, major facilitator family 216 2 35.54 51.99 G 1385 (Bacillales) AEP85070.1 422 acetylornithine aminotransferase 359 2 23.48 127.38 E 1385 (Bacillales) AEP85071.1 316 inositol-1-monophosphatase, putative 316 2 20.82 54.11 G 1385 (Bacillales) AEP85072.1 348 oxidoreductase, zinc-binding protein 293 1.98 26.42 73.4 E 1385 (Bacillales) AEP85075.1 248 YbbA 207 4.01 30.77 51.51 S 1385 (Bacillales) AEP85079.1 529 transcription factor, AraC family 198 1.99 33.4 41.63 K 1385 (Bacillales) AEP85104.1 335 beta-lactamase 150 1.98 31.07 73.64 V 1385 (Bacillales) AEP85105.1 276 transcriptional regulator, putative 256 2 21.48 104.6 K 1385 (Bacillales) AEP85129.1 726 conserved hypothetical protein 153 3.94 29.7 32 M 1385 (Bacillales) AEP85130.1 275 transcriptional regulator, AraC/XylS family 270 2.76 34.93 120.22 K 1385 (Bacillales) AEP85134.1 152 endopeptidase LytF 261 4.99 11.56 107.17 S 86667 (Jeotgalibacillus marinus) AEP85135.1 273 lipoprotein, putative 235 5.41 26.73 38.84 1385 (Bacillales) AEP85140.1 312 extracellular metalloprotease 196 4.78 29.07 37.7 O 1385 (Bacillales) AEP85142.1 294 carboxylesterase NA 275 6.15 29.64 58.53 S 1385 (Bacillales) AEP85146.1 445 erythromycin esterase superfamily 174 5.46 31.95 28.9 S 1385 (Bacillales) AEP85150.1 249 glucosamine-6-phosphate isomerase 313 3.91 20.24 144.2 G 1385 (Bacillales) AEP85162.1 455 putative glucarate transporter 221 3.93 34.15 123.54 G 1385 (Bacillales) AEP85168.1 312 YcbK 324 3.74 16.17 56.92 S 1385 (Bacillales) AEP85174.1 411 proteinase inhibitor I4, serpin 388 2 12.33 85.24 O 1385 (Bacillales) AEP85187.1 128 conserved membrane protein YczC 305 6.16 25.09 49.98 S 1385 (Bacillales) AEP85192.1 386 protein NatB 256 2 36.35 36.75 V 1385 (Bacillales) AEP85203.1 231 zinc uptake system ATP-binding protein zurA 313 2 31.94 152.18 P 1385 (Bacillales) AEP85213.1 93 transcription regulator 281 7.37 20.19 115.92 K 86667 (Jeotgalibacillus marinus) AEP85220.1 659 alpha-amylase 128 2.68 31.01 31.93 G 1385 (Bacillales) AEP85228.1 198 conserved hypothetical protein 365 8.48 21.78 116.86 S 1385 (Bacillales) AEP85230.1 197 tunicamycin resistance protein 337 6.54 35.93 135.28 S 1385 (Bacillales) AEP85234.1 324 LysR substrate binding domain protein 290 7.74 35.84 74.65 K 1385 (Bacillales) AEP85250.1 194 conserved hypothetical protein 208 4.56 13.38 73.02 M 1385 (Bacillales) AEP85251.1 390 conserved hypothetical protein 195 2 30.64 40.77 1385 (Bacillales) AEP85256.1 59 Yx01 protein 269 2.42 34.55 105.62 C 1385 (Bacillales) AEP85273.1 408 transporter, major facilitator family 288 8.09 18.36 34.55 G 1385 (Bacillales) AEP85286.1 473 YclC 144 2 25.98 80.59 H 1385 (Bacillales) AEP85287.1 75 conserved domain protein 189 5.16 20.36 79.75 S 1385 (Bacillales) AEP85289.1 281 hydrolase, alpha/beta fold family, putative 293 2 16.27 83.06 S 1385 (Bacillales) AEP85297.1 488 YclI 140 2.79 33.62 40.03 V 1385 (Bacillales) AEP85308.1 301 YcnC 240 12.62 33.9 33.87 K 1385 (Bacillales) AEP85311.1 104 YczG 244 2 34.17 90.76 K 1385 (Bacillales) AEP85353.1 283 YdaK 215 2.59 35.9 28.16 T 1385 (Bacillales) AEP85364.1 150 YdaT 258 6.49 33.58 93.08 S 1385 (Bacillales) AEP85407.1 248 oxidoreductase 255 2 27.67 121.22 S 1385 (Bacillales) AEP85409.1 375 alpha/beta hydrolase superfamily protein 235 2 17.86 153.09 S 1385 (Bacillales) AEP85413.1 428 amino acid permease family protein 220 1 29.33 49.26 E 1385 (Bacillales) AEP85415.1 191 TetR-family regulatory protein 280 1.93 29.9 107.23 K 1385 (Bacillales) AEP85417.1 337 oxidoreductase, zinc-binding protein 238 2.8 24.52 120.47 C 1385 (Bacillales) AEP85422.1 265 dihydrodipicolinate synthase family protein, putative 314 2.19 30.67 74.18 E 1385 (Bacillales) AEP85426.1 208 transporter, LysE family 336 2 24.22 110.26 E 1385 (Bacillales) AEP85427.1 123 integral membrane protein 185 1.99 32.42 74.43 S 1385 (Bacillales) AEP85434.1 230 transcriptional regulator, GntR family 321 2 27.12 107.91 K 1385 (Bacillales) AEP85440.1 166 caax amino protease family 266 2.83 27.97 42.15 S 1385 (Bacillales) AEP85442.1 237 lipoprotein, putative 228 6.63 31.57 42.76 1385 (Bacillales) AEP85443.1 235 carbonyl reductase 393 1.99 31.59 148.72 S 1385 (Bacillales) AEP85449.1 287 transporter, dme family 261 4.61 22.43 164.99 E, G 1385 (Bacillales) AEP85451.1 141 MaoC like domain, putative 314 2.92 34.55 126.85 I 1385 (Bacillales) AEP85452.1 190 conserved hypothetical protein 253 6.68 19.44 79.18 S 1385 (Bacillales) AEP85464.1 483 two-component sensor histidine kinase YclK 214 2.86 18.93 62.79 T 1385 (Bacillales) AEP85465.1 237 two-component response regulator YclJ 315 4.79 28.87 129.92 T 1385 (Bacillales) AEP85481.1 229 transport protein 306 1.98 35.49 147.31 S 1385 (Bacillales) AEP85482.1 270 transcription regulator of multidrug-efflux transporter 243 4.77 30.61 70.35 K 1385 (Bacillales) AEP85490.1 122 conserved hypothetical protein 207 1.94 33.58 51.98 1385 (Bacillales) AEP85496.1 199 YdgC 301 2 36.67 78.19 K 1385 (Bacillales) AEP85497.1 111 YdgD 133 8.08 34.13 21.6 1385 (Bacillales) AEP85499.1 341 YisS 261 2 15.99 137.63 G 1385 (Bacillales) AEP85500.1 334 HTH-type transcriptional regulator DegA 174 4.23 32.4 49.44 K 1385 (Bacillales) AEP85501.1 447 sugar transporter family protein 150 3.53 36.41 38.56 G 1385 (Bacillales) AEP85502.1 273 D-Tagatose 3-epimerase, putative 368 2 14.03 76.39 G 1385 (Bacillales) AEP85504.1 188 transcriptional regulator, TetR family, putative 301 1.98 30.3 53.87 K 1385 (Bacillales) AEP85508.1 153 transcriptional regulator, MarR family 202 5.72 19.32 39.76 K 1385 (Bacillales) AEP85514.1 107 integral membrane protein 222 1.98 36.63 76.75 S 1385 (Bacillales) AEP85561.1 228 PspA 325 5.75 35.85 134.39 K, T 1385 (Bacillales) AEP85563.1 255 YdjH 283 3.84 31.43 67.2 S 1385 (Bacillales) AEP85565.1 337 Ion channel family 341 4.47 11.43 96.35 S 1385 (Bacillales) AEP85599.1 424 amino acid permease family protein 234 8.36 26.62 57.26 E 1385 (Bacillales) AEP85621.1 386 McrA 241 2.26 23.78 36.45 V 86667 (Jeotgalibacillus marinus) AEP85625.1 306 conserved hypothetical protein 373 1.96 18.66 144.92 1385 (Bacillales) AEP85629.1 194 YezE 347 3.91 25.65 115.61 K 1385 (Bacillales) AEP85630.1 147 YesE 353 2.91 17.49 130.81 S 1385 (Bacillales) AEP85631.1 282 YesF 362 8.02 21.41 137.31 S 1313 (Streptococcus pneumoniae) AEP85635.1 180 acetyltransferase, gnat family 215 2 30.75 61.74 S 1385 (Bacillales) AEP85637.1 577 histidine kinase family protein 236 3.77 32.6 62.58 T 1385 (Bacillales) AEP85638.1 368 response regulator YesM 255 4.61 27.89 42.91 T 1385 (Bacillales) AEP85643.1 761 transcriptional regulator, AraC family protein 208 2.92 26.61 42.83 K 1385 (Bacillales) AEP85645.1 220 YesU 295 3.69 29.89 109.95 S 1385 (Bacillales) AEP85651.1 857 hypothetical protein GYO_0969 259 3.26 32.18 80.95 1385 (Bacillales) AEP85652.1 502 lipoprotein LplA 262 6.39 34.73 87.81 G 1385 (Bacillales) AEP85653.1 318 protein LplB 234 3.59 35.74 116.24 G 1385 (Bacillales) AEP85655.1 446 putative glucosidase LplD 306 2.65 20.83 113.12 G 1385 (Bacillales) AEP85659.1 192 conserved hypothetical protein 266 4.64 29.55 33.31 86667 (Jeotgalibacillus marinus) AEP85663.1 308 putative transport protein YetK 309 4.67 23.83 83.76 S 1385 (Bacillales) AEP85664.1 167 transcriptional regulator, MarR family 294 2.59 15.44 83.57 K 1385 (Bacillales) AEP85665.1 379 FAD binding domain, putative 329 3.49 27.21 67.5 C, H 1385 (Bacillales) AEP85666.1 262 Mrr restriction system protein 390 1.99 16.22 123.02 V 1385 (Bacillales) AEP85692.1 487 conserved hypothetical protein 349 2.99 7.56 44.56 S 1385 (Bacillales) AEP85693.1 266 ferrichrome transport ATP-binding protein FhuC 317 2 34.97 149.36 P 1385 (Bacillales) AEP85694.1 333 iron(III) dicitrate transport system permease protein FecD 216 2 32.83 75.57 P 1385 (Bacillales) AEP85695.1 320 ferrichrome transport system permease protein FhuB 165 3.96 35.06 51.43 P 1385 (Bacillales) AEP85696.1 315 iron(III) dicitrate-binding periplasmic protein 207 3.91 25.43 67.68 P 1385 (Bacillales) AEP85699.1 420 pectate lyase 248 2.97 31.94 91.52 G 1385 (Bacillales) AEP85700.1 478 2-oxoglutarate/malate translocator 275 2 31.96 138.03 P 1385 (Bacillales) AEP85722.1 150 conserved hypothetical protein 221 3.96 22.77 27.92 S 159743 (Paenibacillus terrae) AEP85743.1 317 3-hydroxyisobutyrate dehydrogenase family protein 250 5.98 24.81 85.25 I 1385 (Bacillales) AEP85762.1 449 maltose-6'-phosphate glucosidase 177 2 28.05 109.37 G 1385 (Bacillales) AEP85763.1 254 HTH-type transcriptional regulator GlvR 215 5.88 29.82 59.41 K 1385 (Bacillales) AEP85764.1 527 pts system maltose-specific eiicb component 194 2.67 29.58 99.7 G 1385 (Bacillales) AEP85769.1 314 putative HTH-type transcriptional regulator YfiF 293 3.36 23.68 55.7 K 1385 (Bacillales) AEP85771.1 313 sugar phosphate isomerase/epimerase 259 1.99 26.55 141.9 G 1385 (Bacillales) AEP85772.1 392 oxidoreductase 225 6.52 27.55 126.25 S 1385 (Bacillales) AEP85773.1 388 histidine kinase YfiK 226 12.6 35.59 26.64 T 1385 (Bacillales) AEP85780.1 182 transcriptional regulator 245 4.87 33.03 78.43 K 1385 (Bacillales) AEP85785.1 518 YfiU 215 8.03 29.73 63.52 G 1385 (Bacillales) AEP85786.1 160 YfiV 353 2.95 23.2 72 K 1385 (Bacillales) AEP85801.1 110 conserved hypothetical protein 210 8.75 35.64 33.3 1385 (Bacillales) AEP85802.1 288 alpha/beta hydrolase fold protein 298 1.98 35.45 117.07 S 1385 (Bacillales) AEP85845.1 154 transcriptional regulator, MarR family 330 2.73 21.58 51.54 K 1385 (Bacillales) AEP85857.1 359 ggdef domain protein 192 4.23 35.47 30.92 T 1385 (Bacillales) AEP85898.1 331 protein iolS 301 2 22.58 167.58 C 1385 (Bacillales) AEP85901.1 299 NAD-dependent epimerase/dehydratase 401 2 21.7 125.58 G, M 1385 (Bacillales) AEP85904.1 393 putative aspartate aminotransferase 333 5.47 32.2 124.48 E 1385 (Bacillales) AEP85990.1 323 YhfQ 148 4.59 29.52 30.8 P 1385 (Bacillales) AEP86000.1 299 short chain dehydrogenase family protein 182 5.9 33.38 95.81 S 1385 (Bacillales) AEP86008.1 522 FAD-dependent monooxygenase 225 10.49 25.71 73.18 C, H 1385 (Bacillales) AEP86009.1 175 YhjH 180 2.94 29.77 41.55 K 1385 (Bacillales) AEP86011.1 406 glucose/mannose:H+ symporter 158 2.9 24.05 22.71 G 1385 (Bacillales) AEP86012.1 350 oxidoreductase, gfo/idh/moca family 250 4.55 26.97 36.73 S 1385 (Bacillales) AEP86014.1 441 NTD biosynthesis operon protein NtdA 183 4.38 28.61 40.03 E 1385 (Bacillales) AEP86046.1 342 YisS 276 4.44 32.01 92.66 S 1385 (Bacillales) AEP86055.1 228 sporulation putative 2-phosphosulfolactate phosphatase YitC 368 3.59 31.52 83.8 T 1385 (Bacillales) AEP86058.1 371 sporulation protein YitF 329 3.62 24.68 82.22 M 1385 (Bacillales) AEP86151.1 93 YjcS 108 9.57 29.67 20.25 S 1385 (Bacillales) AEP86156.1 167 acetyltransferase, gnat family 295 3.41 23.99 68.76 J 1385 (Bacillales) AEP86158.1 160 YbaK/ebsC protein 235 4.63 31.95 103.64 S 1385 (Bacillales) AEP86162.1 320 protoheme IX farnesyltransferase 281 9.73 32.71 88.38 O 1385 (Bacillales) AEP86165.1 55 conserved domain protein 92 1.97 26.65 24.61 1385 (Bacillales) AEP86168.1 191 conserved hypothetical protein 137 5.32 32 19.89 1385 (Bacillales) AEP86175.1 392 YjiC 261 3.77 22.2 75.95 C, G 1385 (Bacillales) AEP86197.1 423 cell-division protein 353 5.98 33.16 97.33 O 1385 (Bacillales) AEP86200.1 59 ycii-related domain superfamily 271 3.47 19.4 110.38 S 44251 (Paenibacillus durus) AEP86218.1 275 conserved hypothetical protein 113 2.98 33.97 18.25 1385 (Bacillales) AEP86219.1 311 conserved hypothetical protein 141 2 35.97 20.88 S 1385 (Bacillales) AEP86226.1 147 conserved hypothetical protein 289 1.99 26.06 101.8 S 1385 (Bacillales) AEP86227.1 148 conserved hypothetical protein 272 4.29 29.4 61.96 S 1385 (Bacillales) AEP86232.1 88 conserved hypothetical protein 101 2.86 31.66 19.6 S 1385 (Bacillales) AEP86235.1 192 conserved hypothetical protein 215 5.62 35.81 34.22 S 1385 (Bacillales) AEP86256.1 272 pyrroline-5-carboxylate reductase 3 212 2.48 36.29 38.72 E 1385 (Bacillales) AEP86282.1 367 glycosyl transferase 256 2.87 21.94 49.72 M 1385 (Bacillales) AEP86292.1 199 YkoE 321 2.97 35.95 113.04 S 1385 (Bacillales) AEP86293.1 200 ykof-related family protein 339 3.87 31.48 112.23 S 1385 (Bacillales) AEP86296.1 171 peptidase propeptide and ypeb domain protein 247 3.79 29.69 33.47 S 1385 (Bacillales) AEP86337.1 143 conserved hypothetical protein 193 2.76 30.41 46.71 S 1385 (Bacillales) AEP86349.1 75 endopeptidase LytF 173 3.14 23.22 39.02 M 1385 (Bacillales) AEP86350.1 311 glycosyl hydrolase, family 18 320 3.17 35.36 74.91 G 1385 (Bacillales) AEP86443.1 39 conserved hypothetical protein 96 1.98 25.02 32.07 S 1385 (Bacillales) AEP86698.1 225 polyketide synthesis protein 321 2 21.5 101.92 S 1385 (Bacillales) AEP86699.1 288 malonyl CoA-acyl carrier protein transacylase 114 3.8 24.69 47.24 I 1385 (Bacillales) AEP86700.1 324 polyketide synthesis protein 275 1.99 18.36 89.22 Q 1385 (Bacillales) AEP86701.1 760 malonyl CoA-acyl carrier protein transacylase, putative 123 2.56 26.11 48.17 H 1385 (Bacillales) AEP86702.1 82 conserved domain protein 229 2.73 13.55 91.49 I, Q 1385 (Bacillales) AEP86703.1 415 putative polyketide beta-ketoacyl synthase 264 8.47 19.65 99.83 I, Q 1385 (Bacillales) AEP86704.1 420 TaC protein 255 2.96 25.3 141.96 I 1385 (Bacillales) AEP86705.1 256 conserved hypothetical protein 274 9 22.14 100.69 I 1385 (Bacillales) AEP86706.1 249 conserved hypothetical protein 206 3.59 22.34 94.22 M 1385 (Bacillales) AEP86708.1 4563 KR domain family 132 12.3 29.23 18.38 Q 1385 (Bacillales) AEP86709.1 4260 KR domain family 133 4.97 25.22 22.43 Q 1385 (Bacillales) AEP86713.1 118 conserved hypothetical protein 131 2 18.14 24.16 1385 (Bacillales) AEP86749.1 235 endo-1,4-beta-xylanase A 297 1.99 24.2 139.45 G 1385 (Bacillales) AEP86757.1 144 deoxyuridine 5'-triphosphate nucleotidohydrolase superfamily 295 2.9 35.16 153.37 F 1385 (Bacillales) AEP86761.1 279 thymidylate synthase 270 3.79 32.08 95.08 F 1385 (Bacillales) AEP86817.1 134 YngA protein 251 3.99 34.96 70.5 S 1385 (Bacillales) AEP86829.1 188 acetyltransferase, gnat family, putative 205 2.74 28.05 80.3 J 1385 (Bacillales) AEP86832.1 2611 amino acid adenylation domain protein 86 3.6 23.55 22.39 Q 1385 (Bacillales) AEP86833.1 5365 amino acid adenylation domain protein 124 2.79 23.88 30.1 Q 1385 (Bacillales) AEP86834.1 3971 aminotransferase, class III family 113 2.8 22.95 23.88 Q 1385 (Bacillales) AEP86835.1 399 malonyl CoA-acyl carrier protein transacylase 188 2 26.06 53.39 I 1385 (Bacillales) AEP86844.1 284 HTH-type transcriptional regulator GltR 204 3.31 32.71 67.3 K 1385 (Bacillales) AEP86851.1 122 replication termination protein 153 4 28.92 56.19 L 1385 (Bacillales) AEP86856.1 252 conserved hypothetical protein 213 4.57 29.7 55.92 558169 (Lentibacillus jeotgali) AEP86857.1 330 ABC-type MDR transport system, permease component 183 3.77 18.07 25.71 1385 (Bacillales) AEP86858.1 248 ABC-type MDR transport system, permease component 187 2.9 19.79 39.69 V 1385 (Bacillales) AEP86860.1 183 transcriptional regulator, MarR/EmrR family 258 1.99 27.04 71.93 K 1385 (Bacillales) AEP86862.1 487 carbohydrate kinase, FGGY family 207 2.94 33.44 40.28 G 1385 (Bacillales) AEP86863.1 333 putative D-isomer specific 2-hydroxyacid dehydrogenase 199 10.36 26.38 63.95 C 1385 (Bacillales) AEP86865.1 97 conserved hypothetical protein 132 2 29.24 21.92 1385 (Bacillales) AEP86872.1 232 YoaJ 224 2.5 29.59 89.34 M 1385 (Bacillales) AEP86873.1 225 conserved membrane protein YoaK 266 5.45 33.53 45.68 S 1385 (Bacillales) AEP86874.1 345 pectin lyase 197 5.48 24 73.33 G 1385 (Bacillales) AEP86875.1 228 acetoin(diacetyl) reductase 322 2.7 28.9 153.96 C 1385 (Bacillales) AEP86877.1 238 necrosis and ethylene inducing protein 256 2 21.86 104.91 S 1385 (Bacillales) AEP86887.1 290 putative HTH-type transcriptional regulator YoaU 164 3.86 32.25 40.24 K 1385 (Bacillales) AEP86888.1 292 YoaV 308 3.79 24.25 69.72 S 1385 (Bacillales) AEP86892.1 296 caax amino protease family 249 3.62 13.33 37.47 S 1385 (Bacillales) AEP86897.1 206 chitin binding protein 116 1.99 31.78 41.84 S 1385 (Bacillales) AEP86901.1 213 endo-1,4-beta-xylanase A 275 2.82 31.65 155.31 G 1385 (Bacillales) AEP86905.1 243 beta-glucanase 185 4.77 36.39 83.67 G 1385 (Bacillales) AEP86908.1 210 acetyltransferase, gnat family 255 3.89 16.27 49.59 S 1385 (Bacillales) AEP86932.1 312 YobV 232 4.63 28.08 68.08 K 1385 (Bacillales) AEP86937.1 260 conserved hypothetical protein 297 6.22 32.9 55.8 1385 (Bacillales) AEP86943.1 502 Na+/solute symporter 260 2.59 20.34 112.86 S 1385 (Bacillales) AEP86951.1 114 YozO 361 3.71 22.36 95.07 S 1385 (Bacillales) AEP87010.1 382 3-phytase 251 5.77 33.02 74.97 I 1385 (Bacillales) AEP87011.1 341 polysaccharide biosynthesis protein CapD 273 3.22 35.67 81.19 M 1385 (Bacillales) AEP87053.1 194 xanthine phosphoribosyltransferase 205 2.96 35.02 118.04 F 1385 (Bacillales) AEP87055.1 330 2-keto-3-deoxygluconate permease 251 2.99 32.09 103.59 P 1385 (Bacillales) AEP87056.1 196 khg/kdpg aldolase 250 3.83 34.07 68.64 G 1385 (Bacillales) AEP87058.1 339 HTH-type transcriptional regulator KdgR 232 2 31.21 69.95 K 1385 (Bacillales) AEP87204.1 439 putative NAD-dependent malic enzyme 1 323 2.98 35.11 126.67 C 1385 (Bacillales) AEP87221.1 113 antibiotic biosynthesis monooxygenase family protein 292 7.53 35.36 129.32 S 1385 (Bacillales) AEP87285.1 224 zinc metalloprotease 416 1 6.87 150.12 S 1385 (Bacillales) AEP87286.1 1061 type I restriction-modification system, R subunit 419 1 2.46 136.95 V 1385 (Bacillales) AEP87287.1 425 type I restriction-modification enzyme, S subunit 400 1 2.58 72.62 V 1385 (Bacillales) AEP87288.1 958 type I restriction-modification system, M subunit 428 1 2.3 131.41 V 1385 (Bacillales) AEP87291.1 324 DNA integration/recombination protein 254 1.19 22.52 63.56 L 1385 (Bacillales) AEP87418.1 57 ribosomal protein S21 335 1 33.01 263.7 J 1385 (Bacillales) AEP87453.1 238 YqeB 281 9.43 26.27 68.82 1385 (Bacillales) AEP87457.1 368 alanine racemase, N- domain family 366 1.99 36.32 110.61 E 1385 (Bacillales) AEP87458.1 96 endoribonuclease L-PSP superfamily 351 1 24.6 129.89 J 1385 (Bacillales) AEP87461.1 124 translation initiation inhibitor 347 1.99 32.68 109.73 J 1385 (Bacillales) AEP87486.1 363 two-component sensor histidine kinase 322 1.8 24.32 69.34 T 1385 (Bacillales) AEP87488.1 137 conserved hypothetical protein 384 1.85 13.04 109.41 S 1385 (Bacillales) AEP87492.1 282 transcription antiterminator 228 1 20.84 95.09 K 1385 (Bacillales) AEP87493.1 468 6-phospho-beta-glucosidase 253 1 8.46 136.8 G 1385 (Bacillales) AEP87494.1 617 pts system beta-glucoside-specific eiibca component 211 1 16.08 89.65 G 1385 (Bacillales) AEP87499.1 188 cysteine dioxygenase type I 223 1.97 3.64 60.97 S 1385 (Bacillales) AEP87502.1 244 NADP oxidoreductase, coenzyme f420-dependent 308 2 27.89 130.02 S 1385 (Bacillales) AEP87505.1 246 oxygen-insensitive NADPH nitroreductase 160 1.99 25.7 75.82 C 1385 (Bacillales) AEP87512.1 152 spermine/spermidine acetyltransferase 235 4.55 32.72 81.14 K 1385 (Bacillales) AEP87518.1 189 flavin reductase like domain, putative 400 2 27.07 100.6 S 1385 (Bacillales) AEP87520.1 281 BmrR 228 2.22 32.88 33.87 K 1385 (Bacillales) AEP87522.1 110 MmcQ 344 1.99 25.07 172.56 S 1385 (Bacillales) AEP87524.1 607 serine protease 261 2.73 16.37 107.56 I 1385 (Bacillales) AEP87526.1 176 RNA polymerase sigma factor, SigZ family 316 2.76 10.33 118.75 K 1385 (Bacillales) AEP87527.1 326 aldo/keto reductase 286 7.75 14.72 167.98 C 1385 (Bacillales) AEP87528.1 278 chitosanase 302 2.91 23.15 70.69 M 1385 (Bacillales) AEP87531.1 118 conserved hypothetical protein 296 9.13 8.46 90.85 S 1385 (Bacillales) AEP87532.1 119 conserved hypothetical protein 292 8.5 9.21 96.19 S 1385 (Bacillales) AEP87539.1 134 transcriptional regulator, MerR family 309 5.93 13.39 151.85 K 1385 (Bacillales) AEP87540.1 244 4-carboxymuconolactone decarboxylase domain protein 329 3.34 5.55 153.38 S 1385 (Bacillales) AEP87544.1 275 fructose permease IID component 252 3 11.57 149.39 G 1385 (Bacillales) AEP87545.1 269 fructose permease IIC component 291 2.96 15.52 146.88 G 1385 (Bacillales) AEP87546.1 162 fructose-specific phosphotransferase enzyme IIB component 266 3.95 18.97 125.93 G 1385 (Bacillales) AEP87547.1 146 fructose-specific phosphotransferase enzyme IIA component 248 4.89 16.92 75.67 G 1385 (Bacillales) AEP87548.1 935 sigma-54 interaction domain family 191 5.88 33.04 57.19 K 1385 (Bacillales) AEP87557.1 98 conserved hypothetical protein 351 5.1 21.47 111.46 86667 (Jeotgalibacillus marinus) AEP87560.1 180 methyltransferase domain family 295 6.02 25.42 44.53 Q 1385 (Bacillales) AEP87626.1 240 conserved hypothetical protein 305 7.54 29.23 162.86 K 1385 (Bacillales) AEP87683.1 274 conserved domain protein 224 3.37 34.32 30.36 S 1385 (Bacillales) AEP87722.1 297 hypothetical protein GYO_3119 177 1.99 20.9 18.56 S 1385 (Bacillales) AEP87735.1 324 endo-a1,5-arabinanase 264 4.62 34.06 98.75 G 1385 (Bacillales) AEP87781.1 63 conserved hypothetical protein 154 1.98 29.25 44.05 1385 (Bacillales) AEP87875.1 166 conserved hypothetical protein 177 3.48 15.96 43.1 1385 (Bacillales) AEP87917.1 134 YtkC 157 2.98 34.76 21.52 S 1385 (Bacillales) AEP87961.1 193 YuaD 337 2.91 33.7 107.75 S 1385 (Bacillales) AEP87968.1 87 YubF 285 1.99 33.55 82.32 S 1385 (Bacillales) AEP87969.1 282 Exo-glucosaminidase LytG 109 3.86 20.13 28.51 N, U 1385 (Bacillales) AEP87975.1 69 hypothetical protein GYO_3396 182 1 34.98 55.43 L 86667 (Jeotgalibacillus marinus) AEP87980.1 104 L-rhamnose 1-epimerase 251 2.88 33.5 129.73 S 1385 (Bacillales) AEP87981.1 486 rhamnulokinase 207 2.92 22.09 92.56 G 1385 (Bacillales) AEP88038.1 289 competence regulatory protein ComQ 191 6.98 35.25 21.25 H 1385 (Bacillales) AEP88063.1 69 conserved domain protein 327 2.96 18.57 200.89 S 1385 (Bacillales) AEP88116.1 116 hydroxyisourate hydrolase 323 4.53 20.43 153.27 F 1385 (Bacillales) AEP88121.1 237 cytoChrome c-type biogenesis protein CcdA 204 1 36.71 71.35 O 1385 (Bacillales) AEP88124.1 277 putative xanthine dehydrogenase subunit C 331 2.85 31.2 51.38 C 1385 (Bacillales) AEP88129.1 143 glycosyl transferase family, helical bundle domain protein 166 2.41 13.15 25.21 M 1385 (Bacillales) AEP88132.1 242 HTH-type transcriptional regulator FrlR 198 15.34 35.3 47.01 K 1385 (Bacillales) AEP88169.1 127 YusQ 279 4.18 20.24 114.04 S 1385 (Bacillales) AEP88171.1 295 HTH-type transcriptional regulator GltR 220 4.31 36.42 74.94 K 1385 (Bacillales) AEP88194.1 226 protein LiaH 252 2 33.99 54.24 K, T 1385 (Bacillales) AEP88202.1 263 YvrD 349 5.73 34.77 147.75 S 1385 (Bacillales) AEP88211.1 409 ABC transporter 247 6.8 33.19 61.06 V 1385 (Bacillales) AEP88213.1 397 ABC transporter 152 2 25.33 18.76 V 1385 (Bacillales) AEP88229.1 141 stress response protein YvgO 127 3.95 32.58 28.49 1385 (Bacillales) AEP88243.1 632 membrane protein, putative 224 2.58 32.21 31.16 S 1385 (Bacillales) AEP88244.1 218 transcriptional regulator, GntR family 191 2 31.12 58.35 K 1385 (Bacillales) AEP88248.1 119 multidrug resistance protein, SMR family 292 3.8 36.15 108.86 P 1385 (Bacillales) AEP88249.1 190 transcriptional regulator, TetR family 234 2 28.79 51.79 K 1385 (Bacillales) AEP88258.1 135 transcriptional repressor RghR 244 5.93 34.37 53.85 K 1385 (Bacillales) AEP88259.1 139 YvaO 243 4.47 33.73 47.61 K 1385 (Bacillales) AEP88261.1 566 methyl-accepting chemotaxis protein 246 2.64 33.7 37.35 D 1385 (Bacillales) AEP88262.1 421 subtilin biosynthesis sensor protein SpaK 149 3.89 18.3 37.81 T 1385 (Bacillales) AEP88263.1 220 subtilin biosynthesis regulatory protein SpaR 224 1.98 22.05 103.88 T 1385 (Bacillales) AEP88264.1 203 SpaG 199 3.92 23.56 43.16 P 1385 (Bacillales) AEP88265.1 251 SpaE 218 2 20.89 57.53 S 1385 (Bacillales) AEP88266.1 238 bacitracin transport ATP-binding protein BcrA 195 3.95 28.28 106.63 V 1385 (Bacillales) AEP88269.1 441 subtilin biosynthesis protein SpaC 249 1.98 14.11 50.49 S 1385 (Bacillales) AEP88270.1 614 conserved hypothetical protein 291 2 16.15 82.02 S 1385 (Bacillales) AEP88271.1 1030 putative subtilin biosynthesis protein SpaB 201 3.63 15.2 31.37 S 1385 (Bacillales) AEP88284.1 232 conserved hypothetical protein 211 2.82 30.74 23.68 1385 (Bacillales) AEP88296.1 464 arabinose-proton symporter 265 2.94 29.01 102.98 G 1385 (Bacillales) AEP88303.1 255 glycosyl hydrolase, family 18 283 3.23 34.86 71.54 G 361277 (Terribacillus saccharophilus) AEP88311.1 205 conserved hypothetical protein 181 2 24.82 39.39 S 1385 (Bacillales) AEP88312.1 109 transcriptional regulator, PadR family 285 2 30.39 113.64 K 1385 (Bacillales) AEP88330.1 322 YvfF 234 3.86 29.67 66.42 M 1385 (Bacillales) AEP88334.1 505 YvfB 223 8.54 35.78 33.31 M 1385 (Bacillales) AEP88335.1 344 YveT 176 2.92 33.17 27.52 M 1385 (Bacillales) AEP88348.1 161 phenolic acid decarboxylase 237 2 31.45 159.48 Q 1385 (Bacillales) AEP88349.1 151 conserved hypothetical protein 262 7.12 20.11 98.69 S 1385 (Bacillales) AEP88351.1 451 beta-lactamase 224 5.53 31.25 58.79 V 1385 (Bacillales) AEP88358.1 104 quaternary ammonium compound-resistance protein sugE 371 2.91 32.06 143.35 P 1385 (Bacillales) AEP88363.1 757 glycosyl hydrolase, family 65 148 6.36 35.32 56.21 G 1385 (Bacillales) AEP88364.1 293 YvdJ 169 4.41 19.56 21.23 S 1385 (Bacillales) AEP88365.1 278 YvdI 201 5.54 26.56 120.62 P 1385 (Bacillales) AEP88366.1 435 YvdH 192 4.23 25.03 98.68 P 1385 (Bacillales) AEP88367.1 417 YvdG 180 2.92 24.71 82.31 G 1385 (Bacillales) AEP88368.1 589 alpha amylase, catalytic domain subfamily, putative 172 11.24 31.55 66.17 G 1385 (Bacillales) AEP88369.1 316 YvdE 214 5.9 22.7 98.42 K 1385 (Bacillales) AEP88404.1 194 pectate lyase 268 5.33 16.8 95.78 G 86667 (Jeotgalibacillus marinus) AEP88414.1 157 YvnA 183 2.9 25.43 32.6 K 1385 (Bacillales) AEP88415.1 405 putative cytochrome P450 CypX 330 3.9 21.65 124.66 Q 1385 (Bacillales) AEP88416.1 248 YvmC 290 3.88 19.79 56.93 1385 (Bacillales) AEP88418.1 169 YvmB 170 2.32 31.52 43.85 K 1385 (Bacillales) AEP88431.1 187 transcriptional regulator, TetR family protein 212 2.92 32.06 38.77 K 1385 (Bacillales) AEP88461.1 98 ComF operon protein 2 141 3.54 28.36 25.48 1385 (Bacillales) AEP88471.1 259 conserved membrane-spanning protein, putative 227 1.14 13.07 29.92 1385 (Bacillales) AEP88474.1 219 bacitracin transport ATP-binding protein BcrA 284 1.52 23.59 129.28 P 1385 (Bacillales) AEP88485.1 634 glycosyl transferase, group 2 family protein 108 3.5 10.7 19.2 M 1385 (Bacillales) AEP88491.1 659 putative glycosyltransferase 123 2.96 11.69 20.15 M 1385 (Bacillales) AEP88492.1 506 glycosyl transferase group 1 family protein 196 2.65 17.51 30.77 M 1385 (Bacillales) AEP88498.1 1166 glycosyltransferase 218 1.14 16.48 24.14 M 1385 (Bacillales) AEP88504.1 237 ribitol-5-phosphate cytidylyltransferase 230 2.99 29.94 125.15 I 1385 (Bacillales) AEP88505.1 341 alcohol dehydrogenase, zinc-dependent 197 1.99 28.79 101.36 E 1385 (Bacillales) AEP88506.1 389 putative ribitolphosphotransferase 174 4.48 23.19 40.64 M 1385 (Bacillales) AEP88507.1 623 CDP-Glycerol:Poly(glycerophosphate) glycerophosphotransferase family 185 3.59 22.5 47.68 M 1385 (Bacillales) AEP88518.1 149 PGA biosynthesis protein CapC 346 1.99 35.49 111.39 S 1385 (Bacillales) AEP88543.1 761 acetyl-CoA carboxylase, biotin carboxylase 341 2.62 24.98 109.52 S 1385 (Bacillales) AEP88544.1 288 transcriptional regulator, putative 279 2.61 14.37 43.5 K 1385 (Bacillales) AEP88549.1 183 hypothetical protein GYO_3979 116 1.92 31.19 17.94 1385 (Bacillales) AEP88567.1 212 sortase family protein 264 11.1 28.38 72.79 M 1313 (Streptococcus pneumoniae) AEP88569.1 358 two-component response regulator 248 2.92 29.84 58.53 T 1385 (Bacillales) AEP88570.1 1026 two component sensor histidine kinase, putative YwpD 220 3.01 31.83 49.65 T 1385 (Bacillales) AEP88584.1 153 conserved hypothetical protein 316 7.09 24.08 64.08 1385 (Bacillales) AEP88586.1 116 nitrogen regulatory protein P-II 359 3 33.24 163.4 E 1385 (Bacillales) AEP88588.1 145 sporulation VanZ like family protein YwnJ 143 4.82 22.66 18.27 S 1385 (Bacillales) AEP88592.1 596 NeuB family 265 2.91 17.89 34.53 M 1385 (Bacillales) AEP88600.1 124 urease, beta subunit 359 1.99 24.85 162.77 E 1385 (Bacillales) AEP88601.1 105 urease, gamma subunit 380 4.98 23.52 235.11 E 1385 (Bacillales) AEP88602.1 233 ABC transporter 380 2 21.56 164.59 E 1385 (Bacillales) AEP88603.1 249 ABC transporter 398 2.98 24.3 198.4 E 1385 (Bacillales) AEP88604.1 356 putative membrane protein 368 2.97 24.8 159.96 E 1385 (Bacillales) AEP88605.1 300 ABC transporter 391 2 28.56 200.49 E 1385 (Bacillales) AEP88606.1 418 putative periplasmic binding protein 387 2 29.94 225.68 E 1385 (Bacillales) AEP88607.1 62 conserved domain protein 215 2.86 32.7 75.26 S 1385 (Bacillales) AEP88673.1 209 membrane protein, putative 301 1.96 35.88 71.61 S 1385 (Bacillales) AEP88686.1 448 YwiA protein 297 6.69 17.08 42.77 L 1385 (Bacillales) AEP88688.1 239 YwhQ protein 299 1.99 22.74 65.48 S 1385 (Bacillales) AEP88691.1 427 putative zinc-protease AlbF 304 3.62 16.84 30.75 O 1385 (Bacillales) AEP88700.1 215 ABC transporter, ATP-binding protein 245 1.99 30.05 88.83 P 1385 (Bacillales) AEP88709.1 139 YwhA 284 3.9 35.02 47.69 K 1385 (Bacillales) AEP88725.1 375 putative bacilysin exporter BacE 224 3.97 21.2 19.44 G 1385 (Bacillales) AEP88726.1 472 alanine-anticapsin ligase bacD 231 2.98 27.01 29.57 I 1385 (Bacillales) AEP88729.1 204 bacilysin biosynthesis protein BacA 279 3.93 28.95 47.41 E 1385 (Bacillales) AEP88730.1 412 transporter, major facilitator family 184 2.94 35.57 26.18 G 1385 (Bacillales) AEP88738.1 240 spore coat polysaccharide biosynthesis protein SpsF 271 2 33.04 56.89 M 1385 (Bacillales) AEP88739.1 373 spore coat polysaccharide biosynthesis protein SpsE 315 2.68 31.64 83.03 M 1385 (Bacillales) AEP88740.1 289 spore coat polysaccharide biosynthesis protein and putative acetyltransferase, GNAT family, SpsD 238 2 22.21 22.31 M 1385 (Bacillales) AEP88741.1 389 spore coat polysaccharide biosynthesis protein and putative transaminase SpsC 324 1.99 32.58 144.78 E 1385 (Bacillales) AEP88777.1 223 putative HTH-type transcriptional regulator YwcC 208 3.83 18.91 21.87 1385 (Bacillales) AEP88783.1 385 imelysin family protein 280 2 29.31 70.51 P 1385 (Bacillales) AEP88800.1 286 general stress protein A 124 5.55 30.03 35.13 M 1385 (Bacillales) AEP88837.1 450 citrate/malate transporter 220 4.08 27.1 101.3 C 1385 (Bacillales) AEP88838.1 560 peptidase, M48 family 333 13.36 23.88 26.9 O 1385 (Bacillales) AEP88848.1 213 conserved hypothetical protein 185 4.62 25.45 21.59 1385 (Bacillales) AEP88852.1 406 putative sensor-like histidine kinase protein YxjM 177 5.05 21.08 26.84 T 1385 (Bacillales) AEP88853.1 218 putative sensory transduction protein YxjL 293 2.94 28.87 120.86 T 1385 (Bacillales) AEP88857.1 162 conserved hypothetical protein 316 3.66 31.75 80.71 S 1385 (Bacillales) AEP88874.1 610 pts system beta-glucoside-specific eiibca component 204 2 33.28 82.4 G 1385 (Bacillales) AEP88881.1 382 conserved hypothetical protein 195 3.89 26.55 66.11 S 1385 (Bacillales) AEP88883.1 179 conserved hypothetical protein 258 2 30.95 49.84 S 1385 (Bacillales) AEP88891.1 148 universal stress family protein 350 2.97 27.9 104.35 T 1385 (Bacillales) AEP88898.1 329 oxidoreductase 224 2.63 12.92 112.02 S 1385 (Bacillales) AEP88910.1 275 membrane protein, putative 356 2.97 29.77 89.13 S 1385 (Bacillales) AEP88911.1 370 ethanolamine transporter 288 6.58 29.13 124.07 E 1385 (Bacillales) AEP88916.1 275 amino acid ABC transporter, periplasmic amino acid-binding portion 226 1.95 29.73 77.58 E 1385 (Bacillales) AEP88924.1 117 sporulation protein YxeD 127 5.1 25.21 21.06 1385 (Bacillales) AEP88926.1 321 ferrichrome-binding protein 159 5.89 29.83 53.59 P 1385 (Bacillales) AEP88936.1 438 minor myo-inositol transporter IolF 244 6.88 35.26 77.09 G 1385 (Bacillales) AEP88942.1 251 DNA-binding protein iolR 250 4.22 32.31 87.55 K 1385 (Bacillales) AEP88946.1 461 arabinose-proton symporter 144 4.81 35.46 45.26 G 1385 (Bacillales) AEP88951.1 445 putative aldehyde dehydrogenase AldX 286 2.97 29.83 116.04 C 1385 (Bacillales) AEP88952.1 141 YxaI 279 3.61 26.85 50.78 S 1386078 (Pseudomonas sp. EGD-AK9) AEP88954.1 151 RDD family, putative 255 3.78 32.02 62.5 S 1385 (Bacillales) AEP88956.1 337 quercetin 2,3-dioxygenase 325 2.92 27.36 65.06 S 1385 (Bacillales) AEP88958.1 356 short chain dehydrogenase 291 2 21.17 112.69 S 1385 (Bacillales) AEP88963.1 343 general stress protein 30 212 3.44 30.68 51.49 M 1385 (Bacillales) AEP88972.1 410 Fic protein family 222 2 14.07 60.09 S 1385 (Bacillales) AEP88974.1 450 conserved hypothetical protein 318 2 15.73 96.64 S 1385 (Bacillales) AEP88976.1 622 pts system beta-glucoside-specific eiibca component 256 2 25.45 127.1 G 1385 (Bacillales) AEP88979.1 240 membrane protein, putative 129 2 8.54 49.05 1385 (Bacillales) AEP88980.1 209 ABC transporter, ATP-binding protein 251 3.9 12.87 58.96 P 1385 (Bacillales) AEP88981.1 252 conserved hypothetical protein 187 3.72 7.38 26.73 1385 (Bacillales) AEP88982.1 319 radical SAM domain protein 255 2.88 6.98 41.92 S 1385 (Bacillales) AEP88986.1 680 conserved hypothetical protein 170 1.33 4.03 27.76 1385 (Bacillales) AEP88987.1 1566 hypothetical protein GYO_4430 327 1 5.01 55.67 1385 (Bacillales) AEP88994.1 245 conserved hypothetical protein 204 6.68 35.47 27.59 S 1385 (Bacillales) AEP89002.1 483 heavy metal sensor kinase 264 2.63 21.51 86.52 T 1385 (Bacillales) AEP89003.1 227 response regulator 360 2.95 21.44 203.78 T 1385 (Bacillales) AEP89016.1 67 conserved domain protein 116 2.6 24.09 40.02 1385 (Bacillales) AEP89026.1 125 transcriptional regulator 214 4.02 35.16 101.97 K 1385 (Bacillales) AEP89029.1 436 YybO 294 5.92 33.12 86.29 G 1385 (Bacillales) AEP89030.1 244 transcriptional regulator, MerR family protein 329 6.29 30.29 88.05 K 1385 (Bacillales) AEP89032.1 129 conserved hypothetical protein 224 5.68 36.64 92.32 S 1385 (Bacillales) AEP89038.1 59 conserved hypothetical protein 63 1.95 19.75 19.79 K 2024554 (Virgibacillus sp. IO3-P2-C2) AEP89039.1 220 metallo-beta-lactamase superfamily, putative 233 3.47 33.21 46.93 S 1385 (Bacillales) AEP89040.1 117 cinnamoyl ester hydrolase 253 3.58 34.71 97.11 K 1385 (Bacillales) AEP89045.1 157 conserved hypothetical protein 243 2 31.04 87.04 S 1385 (Bacillales) AEP89046.1 142 conserved hypothetical protein 257 2.61 33.85 94.27 S 1385 (Bacillales) AEP89049.1 299 caax amino protease family 293 5.79 34.1 46.44 S 1385 (Bacillales) AEP89050.1 451 major facilitator superfamily 246 4.15 26.57 82.52 G 1385 (Bacillales) AEP89053.1 311 catabolite control protein B 184 3.69 35.59 50.41 K 1385 (Bacillales) AEP89054.1 252 exodeoxyribonuclease III 118 2 35.96 51.3 L 1385 (Bacillales) AEP89065.1 155 conserved hypothetical protein 175 3.45 19.9 24.5 S 1385 (Bacillales) Supplementary Table 6. COG distribution of putative horizontally transferred genes COG KCTC 3135T TU-B-10T C Energy production and conversion 13 3.34% 16 3.51% G Carbohydrate transport and metabolism 36 9.25% 50 10.96% H Coenzyme transport and metabolism 5 1.29% 5 1.10% D Cell cycle control, cell division, chromosome partitioning 2 0.51% 1 0.22% E Amino acid transport and metabolism 19 4.88% 24 5.26% F Nucleotide transport and metabolism 4 1.03% 4 0.88% I Lipid transport and metabolism 10 2.57% 12 2.63% Q Secondary metabolites biosynthesis, transport and catabolism 8 2.06% 11 2.41% J Translation, ribosomal structure and biogenesis 5 1.29% 6 1.32% K Transcription 53 13.62% 58 12.72% T Signal transduction mechanisms 10 2.57% 20 4.39% L Replication, recombinaiton and repair 17 4.37% 11 2.41% M Cell wall/ membrane/ envelope biogenesis 29 7.46% 27 5.92% N Cell motility 1 0.26% 1 0.22% O Post-translational modification, protein turnover, and chaperones 9 2.31% 7 1.54% P Inorganic ion transport and metabolism 14 3.60% 18 3.95% S Function unknown 99 25.45% 132 28.95% U Intracellular trafficking, secretion, and vesicular transport 2 0.51% 1 0.22% V Defense mechanisms 8 2.06% 13 2.85% N.A. Not matched to database 45 11.57% 39 8.55% Total 389 456 Supplementary Table 7. Antibiotic registance genes of B . subtilis subsp. subtilis KCTC 3135T and B . subtilis subsp. spizizenii TU-B-10T Target name (accession) Target gene number (KCTC 3135T) Target gene number (TU-B-10T) Description of target Efflux pump ABC efflux pump (RF0007) 7 7 ATP-binding cassette (ABC) antibiotic efflux pump [ARO:0010001] msbA (RF0107) 1 1 msbA: ATP-binding cassette (ABC) antibiotic efflux pump [ARO:3000460] tet_MFS_efflux (RF0134) 1 N.A tetracycline resistance MFS efflux pump: selectively pump out tetracycline or tetracycline derivatives [ARO:3000239] norA (RF0109) N.A 1 norA: major facilitator superfamily (MFS) antibiotic efflux pump [ARO:3000391] Beta-lactamase MoxA (RF0105) 1 1 MoxA beta-lactamase (class a) [ARO:3001215] ClassD (RF0056) 1 1 Class D beta-lactamases [ARO:3000075;ARO:3000017] Other vanY (RF0159) N.A 1 VanY: glycopeptide resistance gene [ARO:3000077] AAC3 (RF0002) 1 N.A Aminoglycoside Acetyltransferase (AAC3) [ARO:3000322] mprF (RF0106) 1 1 mprF: peptide antibiotic resistance gene [ARO:3000863] Supplementary Table8. The tarABDF, tagABDF and tarIJKL tblastn similarities from 60 genomes B . subtilis subspecies Strain name tarA tarB tarD tarF tagA tagB tagD tagF tarI tarJ tarK tarL Clade T30 100.00 99.74 100.00 100.00 59.53 49.21 76.74 64.21 100.00 100.00 99.23 99.84 W23 100.00 99.74 100.00 100.00 59.53 49.21 76.74 64.21 100.00 100.00 99.23 99.84 RFWG1A4 100.00 99.48 100.00 72.18 59.53 49.47 83.12 58.30 100.00 100.00 99.23 99.68 NRS 231 100.00 99.74 100.00 100.00 59.53 49.21 76.74 64.21 100.00 100.00 99.23 99.84 B . subtilis subsp. spizizenii JCM 2499 100.00 99.74 100.00 99.65 59.53 49.21 76.74 69.96 100.00 100.00 95.79 99.69 BST 100.00 99.74 100.00 100.00 59.53 49.21 76.74 64.21 100.00 100.00 99.23 99.84 ATCC 6633 100.00 99.74 100.00 100.00 59.53 49.21 76.74 64.21 100.00 100.00 99.23 99.84 MJ01 97.28 95.04 96.12 64.97 59.53 48.94 78.29 55.99 99.16 100.00 97.17 99.19 B . subtilis subsp. inaquosorum KCTC 13429T 96.89 93.99 96.12 65.48 59.92 49.21 77.52 55.21 99.16 99.71 96.40 98.38 B . subtilis subsp. spizizenii HUK15 97.67 95.30 82.95 30.10 59.92 48.41 73.64 31.25 99.16 100.00 97.43 98.71 HJ5 94.55 93.43 95.35 72.54 59.92 48.79 77.52 55.47 98.73 99.71 94.86 98.38 XF-1 94.55 93.30 95.35 72.54 59.92 49.59 77.52 55.47 98.73 99.71 94.86 98.38 A BSD-2 94.55 93.21 95.35 72.54 59.92 48.94 77.52 55.47 98.73 99.71 94.86 98.38 BAB-1 94.55 93.21 95.35 72.54 59.92 48.94 77.52 55.47 98.73 99.71 94.86 98.38 UD1022 94.55 93.99 94.57 64.72 59.92 48.94 78.29 54.95 98.73 99.71 94.86 98.38 B . subtilis subsp. subtilis YP1 94.55 92.43 95.35 64.72 59.53 48.94 77.52 54.95 99.16 99.71 94.86 98.38 HJ0-6 94.16 92.95 96.12 93.15 59.53 48.94 76.74 63.28 98.73 99.71 95.12 98.38 OH 131.1 94.16 92.95 96.12 93.15 59.53 48.94 76.74 63.28 98.73 99.71 95.12 36.13 BSP1 94.55 92.69 96.10 64.72 59.53 48.94 83.12 54.95 98.31 99.71 95.41 98.44 D12-5 94.55 92.69 95.35 64.72 59.53 48.94 76.74 54.95 98.31 99.71 95.12 98.38 JS 94.94 92.15 96.90 69.44 60.31 49.47 75.97 60.94 98.31 99.41 95.12 98.06 B . subtilis subsp. inaquosorum DE111 96.89 93.73 75.19 58.46 59.92 49.87 90.70 56.85 99.16 99.71 96.40 98.38 B . subtilis subsp. spizizenii TU-B-10T 97.67 94.78 75.19 59.90 59.92 47.88 89.15 54.10 99.58 100.00 97.17 98.87 BEST195 60.16 51.34 75.97 62.34 62.50 52.62 93.80 57.92 33.19 24.64 30.23 31.69 BS38 60.16 50.53 75.97 62.34 62.50 52.52 93.80 57.92 33.19 24.92 30.23 31.69 29R7-12 60.16 50.53 75.97 62.34 62.50 52.52 93.80 57.92 33.19 24.64 30.23 31.69 B . subtilis subsp. subtilis CGMCC 2108 60.16 50.53 75.97 62.34 62.50 52.52 93.80 57.92 33.19 24.64 30.23 31.69 HRBS-10TDI13 60.16 50.53 75.97 62.34 62.50 52.52 93.80 57.92 33.19 24.64 30.23 31.69 KH2 60.16 50.53 75.97 62.34 62.50 52.52 93.80 57.92 33.19 24.64 30.23 31.69 B . subtilis subsp. inaquosorum J-5 53.31 52.86 75.19 63.78 66.41 58.01 91.47 75.62 33.19 25.33 31.53 32.97 RFWG1A3 62.92 53.58 76.74 61.98 94.61 85.71 100.00 93.98 31.72 24.64 31.49 32.41 RFWG4C10 62.92 51.32 76.74 61.98 94.26 87.14 100.00 93.98 31.72 24.64 30.08 32.41 B . subtilis subsp. spizizenii RFWG5B15 60.31 51.32 76.74 61.98 95.31 87.14 100.00 93.98 31.72 24.64 30.08 32.41 DV1-B-1 59.92 49.35 76.74 62.24 95.31 87.14 100.00 92.48 31.88 24.29 30.90 33.61 RO-NN-1 59.53 50.14 76.74 63.43 100.00 99.73 100.00 99.60 33.19 24.29 32.14 31.23 TOA 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 TO-A JPC 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 168G 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 168 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 3NA 59.53 48.94 76.74 63.43 100.00 99.74 100.00 100.00 33.19 24.92 31.82 31.87 6051-HGW 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 AG1839 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 BEST7003 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 25.00 32.14 31.87 BS16045 66.67 47.42 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 28.36 27.61 B BS34A 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 BS49 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 B . subtilis subsp. subtilis CU1050 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 delta6 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 JH642 substr. AG174 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 KCTC 1028 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 KCTC 3135T 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 LM 4-2 59.53 49.21 76.74 62.76 100.00 100.00 100.00 99.60 33.19 24.60 32.14 31.87 PS832 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 PY79 59.53 49.86 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.23 QB928 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 SZMC 6179J 59.53 49.21 76.74 63.43 100.00 100.00 100.00 100.00 33.19 24.92 32.14 31.87 BSn5 59.53 49.86 76.74 62.76 100.00 100.00 100.00 99.46 33.19 24.92 32.14 31.23 SG6 59.53 49.21 76.74 62.76 99.22 99.74 100.00 99.58 33.19 24.92 32.14 31.87 VV2 65.37 65.88 66.67 30.63 57.98 49.59 68.99 27.34 98.73 99.71 94.60 98.38 S . aureus subsp. aureus DSM 20231T 34.67 29.55 64.29 47.09 32.89 31.93 69.53 48.70 62.71 60.47 34.28 47.11