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Characterisation of Novel Isosaccharinic Acid Degrading Bacteria and Communities
University of Huddersfield Repository Kyeremeh, Isaac Ampaabeng Characterisation of Novel Isosaccharinic Acid Degrading Bacteria and Communities Original Citation Kyeremeh, Isaac Ampaabeng (2018) Characterisation of Novel Isosaccharinic Acid Degrading Bacteria and Communities. Doctoral thesis, University of Huddersfield. This version is available at http://eprints.hud.ac.uk/id/eprint/34509/ The University Repository is a digital collection of the research output of the University, available on Open Access. Copyright and Moral Rights for the items on this site are retained by the individual author and/or other copyright owners. Users may access full items free of charge; copies of full text items generally can be reproduced, displayed or performed and given to third parties in any format or medium for personal research or study, educational or not-for-profit purposes without prior permission or charge, provided: • The authors, title and full bibliographic details is credited in any copy; • A hyperlink and/or URL is included for the original metadata page; and • The content is not changed in any way. For more information, including our policy and submission procedure, please contact the Repository Team at: [email protected]. http://eprints.hud.ac.uk/ Characterisation of Novel Isosaccharinic Acid Degrading Bacteria and Communities Isaac Ampaabeng Kyeremeh, MSc (Hons) A thesis submitted to the University of Huddersfield in partial fulfilment of the requirements for the degree of Doctor of Philosophy Department of Biological Sciences September 2017 i Acknowledgement Firstly, I would like to thank Almighty God for His countenance and grace all these years. ‘I could do all things through Christ who strengthens me’ (Philippians 4:1) Secondly, my heartfelt gratitude and appreciation go to my main supervisor Professor Paul N. -
Table S5. the Information of the Bacteria Annotated in the Soil Community at Species Level
Table S5. The information of the bacteria annotated in the soil community at species level No. Phylum Class Order Family Genus Species The number of contigs Abundance(%) 1 Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus 1749 5.145782459 2 Bacteroidetes Cytophagia Cytophagales Hymenobacteraceae Hymenobacter Hymenobacter sedentarius 1538 4.52499338 3 Gemmatimonadetes Gemmatimonadetes Gemmatimonadales Gemmatimonadaceae Gemmatirosa Gemmatirosa kalamazoonesis 1020 3.000970902 4 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas indica 797 2.344876284 5 Firmicutes Bacilli Lactobacillales Streptococcaceae Lactococcus Lactococcus piscium 542 1.594633558 6 Actinobacteria Thermoleophilia Solirubrobacterales Conexibacteraceae Conexibacter Conexibacter woesei 471 1.385742446 7 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas taxi 430 1.265115184 8 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas wittichii 388 1.141545794 9 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas sp. FARSPH 298 0.876754244 10 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sorangium cellulosum 260 0.764953367 11 Proteobacteria Deltaproteobacteria Myxococcales Polyangiaceae Sorangium Sphingomonas sp. Cra20 260 0.764953367 12 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas panacis 252 0.741416341 -
Fictibacillus Phosphorivorans Gen. Nov., Sp. Nov. and Proposal to Reclassify
International Journal of Systematic and Evolutionary Microbiology (2013), 63, 2934–2944 DOI 10.1099/ijs.0.049171-0 Fictibacillus phosphorivorans gen. nov., sp. nov. and proposal to reclassify Bacillus arsenicus, Bacillus barbaricus, Bacillus macauensis, Bacillus nanhaiensis, Bacillus rigui, Bacillus solisalsi and Bacillus gelatini in the genus Fictibacillus Stefanie P. Glaeser,1 Wolfgang Dott,2 Hans-Ju¨rgen Busse3 and Peter Ka¨mpfer1 Correspondence 1Institut fu¨r Angewandte Mikrobiologie, Justus-Liebig-Universita¨t Giessen, D-35392 Giessen, Germany Peter Ka¨mpfer 2Institut fu¨r Hygiene und Umweltmedizin, RWTH Aachen, Germany peter.kaempfer 3Institut fu¨r Bakteriologie, Mykologie und Hygiene, Veterina¨rmedizinische Universita¨t, A-1210 Wien, @umwelt.uni-giessen.de Austria A Gram-positive-staining, aerobic, endospore-forming bacterium (Ca7T) was isolated from a bioreactor showing extensive phosphorus removal. Based on 16S rRNA gene sequence similarity comparisons, strain Ca7T was grouped in the genus Bacillus, most closely related to Bacillus nanhaiensis JSM 082006T (100 %), Bacillus barbaricus V2-BIII-A2T (99.2 %) and Bacillus arsenicus Con a/3T (97.7 %). Moderate 16S rRNA gene sequence similarities were found to the type strains of the species Bacillus gelatini and Bacillus rigui (96.4 %), Bacillus macauensis (95.1 %) and Bacillus solisalsi (96.1 %). All these species were grouped into a monophyletic cluster and showed very low sequence similarities (,94 %) to the type species of the genus Bacillus, Bacillus subtilis.Thequinonesystemof strain Ca7T consists predominantly of menaquinone MK-7. The polar lipid profile exhibited the major compounds diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. In addition, minor compounds of an unidentified phospholipid and an aminophospholipid were detected. No glycolipids were found in strain Ca7T, which was consistent with the lipid profiles of B. -
The Natural Product Biosynthetic Potential of Red Sea Nudibranch Microbiomes
The natural product biosynthetic potential of Red Sea nudibranch microbiomes Samar M. Abdelrahman1,2, Nastassia V. Patin3,4, Amro Hanora5, Akram Aboseidah2, Shimaa Desoky2, Salha G. Desoky2, Frank J. Stewart3,4,6 and Nicole B. Lopanik1,3 1 School of Earth and Atmospheric Sciences, Georgia Institute of Technology, Atlanta, GA, USA 2 Faculty of Science, Suez University, Suez, Egypt 3 School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA 4 Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, GA, USA 5 Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt 6 Department of Microbiology and Immunology, Montana State University, Bozeman, MT, USA ABSTRACT Background: Antibiotic resistance is a growing problem that can be ameliorated by the discovery of novel drug candidates. Bacterial associates are often the source of pharmaceutically active natural products isolated from marine invertebrates, and thus, important targets for drug discovery. While the microbiomes of many marine organisms have been extensively studied, microbial communities from chemically-rich nudibranchs, marine invertebrates that often possess chemical defences, are relatively unknown. Methods: We applied both culture-dependent and independent approaches to better understand the biochemical potential of microbial communities associated with nudibranchs. Gram-positive microorganisms isolated from nudibranchs collected in the Red Sea were screened for antibacterial and antitumor activity. To assess their biochemical potential, the isolates were screened for the presence of natural product biosynthetic gene clusters, including polyketide synthase (PKS) and Submitted 6 August 2020 non-ribosomal peptide synthetase (NRPS) genes, using PCR. The microbiomes of Accepted 18 November 2020 the nudibranchs were investigated by high-throughput sequencing of 16S rRNA Published 4 February 2021 amplicons. -
Molecular Phylogenetic Analyses of Diverse Cntl Alone (A) and Cntlm (B) Amino Acid Sequences from Bacteria
Electronic Supplementary Material (ESI) for Metallomics. This journal is © The Royal Society of Chemistry 2020 Supplementary Figures A B 96 Paenibacillus amylolyticus NBRC-15957 100 Paenibacillus amylolyticus NBRC-15957 49 Paenibacillus pabuli NBRC13638 91 Paenibacillus pabuli NBRC13638 28 50 Bacillus gaemokensis BL3-6 Bacillus gaemokensis BL3-6 67 99 Paenibacillus mucilaginosus K02-(B2K11200) Paenibacillus mucilaginosus K02-(B2K11200) 56 Paenibacillus vortex V453 98 Paenibacillus vortex V453 Lysinibacillus sphaericus C3-41 Lysinibacillus sphaericus C3-41 73 97 99 Lysinibacillus xylanilyticus JKR-42 100 Lysinibacillus xylanilyticus JKR-42 Actinosynnema mirum DSM43827 Actinosynnema mirum DSM43827 Austwickia chelonae NBRC105200 Austwickia chelonae NBRC105200 97 100 99 100 91 Glutamicibacter mysorens NBRC103060 98 Glutamicibacter mysorens NBRC103060 100 Arthrobacter arilaitensis RE117 100 Arthrobacter arilaitensis RE117 Staphylococcus pseudintermedius LMG-22219 Staphylococcus pseudintermedius LMG-22219 Staphylococcus epidermidis ATCC-12228 Staphylococcus epidermidis ATCC-12228 100 100 99 Staphylococcus aureus Mu50 100 Staphylococcus aureus Mu50 100 100 Staphylococcus argenteus 3688STDY6125130 100 Staphylococcus argenteus 3688STDY6125130 100 Fusobacterium varium NCTC10560 Fusobacterium mortiferum ATCC-9817 96 Fusobacterium ulcerans NCTC12112 100 Fusobacterium varium NCTC10560 Fusobacterium mortiferum ATCC-9817 100 Fusobacterium ulcerans NCTC12112 100 Fictibacillus phosphorivorans Ca7 100 Fictibacillus phosphorivorans Ca7 61 Fictibacillus arsenicus -
Adedayo OA.Pdf (3.909Mb)
GENETIC POLYMORPHISM OF PREVALENT ROOT NODULES BACTERIAL STRAIN FROM BAMBARA NUT (INDIGENOUS AFRICAN LEGUME) OAADEDAYO orcid.org/0000-0002-2151-6474 Dissertation submitted in fulfilment of the requirements for the degree Magister in Biology at Mafikeng Campus of the North-West University Supervisor: Prof. Olubukola 0 . Babalola Graduation October 2017 Student number: 27048187 http://dspace.nwu.ac.za/ NORTH-WEST UNIVERSITY ® 11111 YUNIBESITI YA BOKONE-BOPHIRIMA ...., NOORDWES·UNIVERSITEIT It all starts here ™ " GENETIC POLYMORPHISM OF PREVALENT ROOT NODULES BACTERIAL STRAIN FROM BAMBARA GROUNDNUT (INDIGENOUS AFRICAN LEGUME) BY OLALEKAN AYODELE ADEDAYO A Dissertation Submitted in Fulfilment of the requirements fo r the degree MASTER OF SCIENCE (BIOLOGY) DEPARTMENT OF BIOLOGICAL SCIENCES, FACULTY OF SCIENCE, AGRICULTURE AND TECHNOLOGY, NORTH-WEST UNIVERSITY, MAFIKENG CAMPUS, SOUTH AFRICA Supervisor: Professor Olubukola O. Babalola 2016 DECLARATION I, the undersigned, declare that this disse1iation submitted to the North-West University for the degree of Masters of Science in Biology in the Faculty of Science, Agriculture and Technology, School of Environmental and Health Sciences, and the work contained herein is my original work with exception of the citations and that this work has not been submitted at any other University in part or entirety for the award of any degree. STUDENT NAME Olalekan Ayodele ADEDAYO SIGNATURE ................................. DATE ....................................... SUPERVISOR'S NAME Professor Olubukola BABALOLA SIGNATURE ................................. DATE ....................................... 2 DEDICATION This dissertation is dedicated to the Almighty God who is the beginning and ending, the custodian of wisdom, knowledge and understanding, and for sparing my li fe to achieve this task to Him alone be praised. 3 ACKNOWLEDGEMENTS I would like to express my gratitude to the following people for their assistance. -
Soil Vanadium(V)-Reducing Related Bacteria Drive Community Response to Vanadium Pollution from a Smelting Plant Over Multiple Gradients
Downloaded from orbit.dtu.dk on: Sep 25, 2021 Soil vanadium(V)-reducing related bacteria drive community response to vanadium pollution from a smelting plant over multiple gradients Wang, Song; Zhang, Baogang; Li, Tingting; Li, Zongyan; Fu, Jie Published in: Environment International Link to article, DOI: 10.1016/j.envint.2020.105630 Publication date: 2020 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Wang, S., Zhang, B., Li, T., Li, Z., & Fu, J. (2020). Soil vanadium(V)-reducing related bacteria drive community response to vanadium pollution from a smelting plant over multiple gradients. Environment International, 138, [105630]. https://doi.org/10.1016/j.envint.2020.105630 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Environment International 138 (2020) 105630 -
Fruit Wastes
IJAMBR 3 (2015) 96-103 ISSN 2053-1818 Bacterial quality of postharvest Irvingia gabonensis (Aubry-Lecomte ex O’Rorke) fruit wastes Ebimieowei Etebu* and Gloria Tungbulu Department of Biological Sciences, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria. Article History ABSTRACT Received 01 October, 2015 Irvingia gabonensis is an economically important fruit tree. Although the fungal Received in revised form 28 postharvest quality of its fruits have been studied, bacteria associated with its October, 2015 Accepted 03 November, 2015 decay are yet unknown. Hence in this research, the bacterial quality of Irvingia fruit wastes was studied during 0, 3, 6 and 9 days after harvest (DAH). The Keywords: results obtained show that postharvest I. gabonensis fruits decayed with time. Irvingia gabonensis, Fruit weight and pH were significantly (P=0.05) influenced by DAH. Mean fruit Bacterial species weight at 0, 3, 6 and 9 DAH was 31.99, 29.46, 26.56 and 23.37 g respectively. Postharvest, Mean pH value decreased from 6.42 at 0 DAH to 6.31, 6.22 and 6.17 at 3, 6 and 9 Decay, DAH respectively. Phylogenetic analysis of 16S rRNA partial gene sequences Phylogenetic analysis. showed that bacterial species related to Bacillus, Enterobacter, Oceanobacillus and Staphylococcus were obtained from I. gabonensis fruits up to 3 DAH but not beyond. Whilst recommending proper washing of fresh Irvingia fruits to avoid Article Type: food poisoning, findings of this work offer the potential use of I. gabonensis fruit Full Length Research Article wastes as substrate for antibiotics production. ©2015 BluePen Journals Ltd. All rights reserved INTRODUCTION Irvingia gabonensis (Aubry-Lecomte ex O’Rorke) is a most known uses and is therefore considered the most highly, economically important fruit tree native to most valuable component of the fruit. -
Patterns of Horizontal Gene Transfer Into the Geobacillus Clade
Imperial College London London Institute of Medical Sciences Patterns of Horizontal Gene Transfer into the Geobacillus Clade Alexander Dmitriyevich Esin September 2018 Submitted in part fulfilment of the requirements for the degree of Doctor of Philosophy of Imperial College London For my grandmother, Marina. Without you I would have never been on this path. Your unwavering strength, love, and fierce intellect inspired me from childhood and your memory will always be with me. 2 Declaration I declare that the work presented in this submission has been undertaken by me, including all analyses performed. To the best of my knowledge it contains no material previously published or presented by others, nor material which has been accepted for any other degree of any university or other institute of higher learning, except where due acknowledgement is made in the text. 3 The copyright of this thesis rests with the author and is made available under a Creative Commons Attribution Non-Commercial No Derivatives licence. Researchers are free to copy, distribute or transmit the thesis on the condition that they attribute it, that they do not use it for commercial purposes and that they do not alter, transform or build upon it. For any reuse or redistribution, researchers must make clear to others the licence terms of this work. 4 Abstract Horizontal gene transfer (HGT) is the major driver behind rapid bacterial adaptation to a host of diverse environments and conditions. Successful HGT is dependent on overcoming a number of barriers on transfer to a new host, one of which is adhering to the adaptive architecture of the recipient genome. -
The Ancient Roots of Nicotianamine: Diversity, Role, Regulation and Evolution of Nicotianamine-Like Metallophores Clémentine Laffont, Pascal Arnoux
The ancient roots of nicotianamine: diversity, role, regulation and evolution of nicotianamine-like metallophores Clémentine Laffont, Pascal Arnoux To cite this version: Clémentine Laffont, Pascal Arnoux. The ancient roots of nicotianamine: diversity, role, regulation and evolution of nicotianamine-like metallophores. Metallomics, Royal Society of Chemistry, 2020, 12 (10), pp.1480-1493. 10.1039/D0MT00150C. cea-03125731 HAL Id: cea-03125731 https://hal-cea.archives-ouvertes.fr/cea-03125731 Submitted on 11 Feb 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. The ancient roots of nicotianamine: diversity, role, regulation and evolution of nicotianamine-like metallophores. Clémentine Laffont, Pascal Arnoux Aix Marseille Univ, CEA, CNRS, BIAM, Saint Paul-Lez-Durance, France F-13108. E-mails : [email protected] ; [email protected] ORCID : C. Laffont : 0000-0003-3067-1369 P. Arnoux: 0000-0003-4609-4893 Clémentine Pascal Arnoux Laffont obtained a received his PhD master’s degree in from the University environmental of Paris XI, Orsay microbiology at (France) in 2000. Université de Pau After postdoctoral et des Pays de positions at Toronto l’Adour (France). University (Canada) Since 2017, she and at the CEA Clémentine Laffont continued as a Pascal Arnoux Cadarache (France), PhD student he obtained a under the supervision of Pascal Arnoux at permanent position at the CEA in the the Molecular and Environmental Molecular and Environmental Microbiology Microbiology (MEM) team at the CEA – (MEM) team. -
Isolation and Diversity of Sediment Bacteria in The
bioRxiv preprint doi: https://doi.org/10.1101/638304; this version posted May 14, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Isolation and Diversity of Sediment Bacteria in the 2 Hypersaline Aiding Lake, China 3 4 Tong-Wei Guan, Yi-Jin Lin, Meng-Ying Ou, Ke-Bao Chen 5 6 7 Institute of Microbiology, Xihua University, Chengdu 610039, P. R. China. 8 9 Author for correspondence: 10 Tong-Wei Guan 11 Tel/Fax: +86 028 87720552 12 E-mail: [email protected] 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 bioRxiv preprint doi: https://doi.org/10.1101/638304; this version posted May 14, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 29 Abstract A total of 343 bacteria from sediment samples of Aiding Lake, China, were isolated using 30 nine different media with 5% or 15% (w/v) NaCl. The number of species and genera of bacteria recovered 31 from the different media significantly varied, indicating the need to optimize the isolation conditions. 32 The results showed an unexpected level of bacterial diversity, with four phyla (Firmicutes, 33 Actinobacteria, Proteobacteria, and Rhodothermaeota), fourteen orders (Actinopolysporales, 34 Alteromonadales, Bacillales, Balneolales, Chromatiales, Glycomycetales, Jiangellales, Micrococcales, 35 Micromonosporales, Oceanospirillales, Pseudonocardiales, Rhizobiales, Streptomycetales, and 36 Streptosporangiales), including 17 families, 41 genera, and 71 species. -
Targeting the ESKAPE Pathogens by Botanical and Microbial Approaches
University of South Florida Scholar Commons Graduate Theses and Dissertations Graduate School March 2020 Targeting the ESKAPE Pathogens by Botanical and Microbial Approaches Emily Dilandro University of South Florida Follow this and additional works at: https://scholarcommons.usf.edu/etd Part of the Microbiology Commons Scholar Commons Citation Dilandro, Emily, "Targeting the ESKAPE Pathogens by Botanical and Microbial Approaches" (2020). Graduate Theses and Dissertations. https://scholarcommons.usf.edu/etd/8186 This Thesis is brought to you for free and open access by the Graduate School at Scholar Commons. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Scholar Commons. For more information, please contact [email protected]. Targeting the ESKAPE Pathogens by Botanical and Microbial Approaches by Emily DiLandro A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science Department of Cell Biology, Microbiology & Molecular Biology College of Arts and Sciences University of South Florida Major professor: Lindsey N. Shaw, Ph.D. Prahathees Eswara, Ph.D. Larry Dishaw, Ph.D. Date of Approval: March 11, 2019 Keywords: Cinnamaldehyde, Secondary Metabolites Copywrite © 2020, Emily DiLandro Table of Contents List of Tables ................................................................................................................... iii List of Figures .................................................................................................................