Dynamics of Bacterial Community Structure in the Rhizosphere and Root Nodule of Soybean: Impacts of Growth Stages and Varieties
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UNIVERSIDAD POLITÉCNICA DE CARTAGENA ESCUELA TÉCNICA SUPERIOR DE INGENIERÍA AGRONÓMICA DEPARTAMENTO DE PRODUCCIÓN VEGETAL INGENIERO AGRÓNOMO PROYECTO FIN DE CARRERA: “AISLAMIENTO E IDENTIFICACIÓN DE LOS RIZOBIOS ASOCIADOS A LOS NÓDULOS DE ASTRAGALUS NITIDIFLORUS”. Realizado por: Noelia Real Giménez Dirigido por: María José Vicente Colomer Francisco José Segura Carreras Cartagena, Julio de 2014. ÍNDICE GENERAL 1. Introducción…………………………………………………….…………………………………………………1 1.1. Astragalus nitidiflorus………………………………..…………………………………………………2 1.1.1. Encuadre taxonómico……………………………….…..………………………………………………2 1.1.2. El origen de Astragalus nitidiflorus………………………………………………………………..4 1.1.3. Descripción de la especie………..…………………………………………………………………….5 1.1.4. Biología…………………………………………………………………………………………………………7 1.1.4.1. Ciclo vegetativo………………….……………………………………………………………………7 1.1.4.2. Fenología de la floración……………………………………………………………………….9 1.1.4.3. Sistema de reproducción……………………………………………………………………….10 1.1.4.4. Dispersión de los frutos…………………………………….…………………………………..11 1.1.4.5. Nodulación con Rhizobium…………………………………………………………………….12 1.1.4.6. Diversidad genética……………………………………………………………………………....13 1.1.5. Ecología………………………………………………………………………………………………..…….14 1.1.6. Corología y tamaño poblacional……………………………………………………..…………..15 1.1.7. Protección…………………………………………………………………………………………………..18 1.1.8. Amenazas……………………………………………………………………………………………………19 1.1.8.1. Factores bióticos…………………………………………………………………………………..19 1.1.8.2. Factores abióticos………………………………………………………………………………….20 1.1.8.3. Factores antrópicos………………..…………………………………………………………….21 -
Actes Congrès MICROBIOD 1
Actes du congrès international "Biotechnologie microbienne au service du développement" (MICROBIOD) Marrakech, MAROC 02-05 Novembre 2009 "Ce travail est publié avec le soutien du Ministère de l'Education Nationale, de l'Enseignement Supérieur, de la Formation des Cadres et de la Recherche Scientifique et du CNRST". MICROBIONA Edition www.ucam.ac.ma/microbiona 1 1 Mot du Comité d'organisation Cher (e) membre de MICROBIONA Cher(e) Participant(e), Sous le Haut Patronage de sa Majesté le Roi Mohammed VI, l'Association MICROBIONA et la Faculté des Sciences Semlalia, Université Cadi Ayyad, organisent du 02 au 05 Novembre 2009, en collaboration avec la Société Française de Microbiologie, le Pôle de Compétences Eau et Environnement et l'Incubateur Universitaire de Marrakech, le congrès international "Biotechnologie microbienne au service du développement" (MICROBIOD). Cette manifestation scientifique spécialisée permettra la rencontre de chercheurs de renommée internationale dans le domaine de la biotechnologie microbienne. Le congrès MICROBIOD est une opportunité pour les participants de mettre en relief l’importance socio-économique et environnementale de la valorisation et l’application de nouvelles techniques de biotechnologie microbienne dans divers domaines appliquées au développement et la gestion durable des ressources, à savoir l’agriculture, l'alimentation, l'environnement, la santé, l’industrie agro-alimentaire et le traitement et recyclage des eaux et des déchets par voie microbienne. Ce congrès sera également l’occasion pour les enseignants-chercheurs et les étudiants-chercheurs marocains d’actualiser leurs connaissances dans le domaine des biotechnologies microbiennes, qui serviront à l’amélioration de leurs recherches et enseignements. Le congrès MICROBIOD sera également l’occasion pour certains de nos collaborateurs étrangers de contribuer de près à la formation de nos étudiants en biotechnologie microbienne et ceci par la discussion des protocoles de travail, des méthodes d’analyse et des résultats obtenus. -
Revised Taxonomy of the Family Rhizobiaceae, and Phylogeny of Mesorhizobia Nodulating Glycyrrhiza Spp
Division of Microbiology and Biotechnology Department of Food and Environmental Sciences University of Helsinki Finland Revised taxonomy of the family Rhizobiaceae, and phylogeny of mesorhizobia nodulating Glycyrrhiza spp. Seyed Abdollah Mousavi Academic Dissertation To be presented, with the permission of the Faculty of Agriculture and Forestry of the University of Helsinki, for public examination in lecture hall 3, Viikki building B, Latokartanonkaari 7, on the 20th of May 2016, at 12 o’clock noon. Helsinki 2016 Supervisor: Professor Kristina Lindström Department of Environmental Sciences University of Helsinki, Finland Pre-examiners: Professor Jaakko Hyvönen Department of Biosciences University of Helsinki, Finland Associate Professor Chang Fu Tian State Key Laboratory of Agrobiotechnology College of Biological Sciences China Agricultural University, China Opponent: Professor J. Peter W. Young Department of Biology University of York, England Cover photo by Kristina Lindström Dissertationes Schola Doctoralis Scientiae Circumiectalis, Alimentariae, Biologicae ISSN 2342-5423 (print) ISSN 2342-5431 (online) ISBN 978-951-51-2111-0 (paperback) ISBN 978-951-51-2112-7 (PDF) Electronic version available at http://ethesis.helsinki.fi/ Unigrafia Helsinki 2016 2 ABSTRACT Studies of the taxonomy of bacteria were initiated in the last quarter of the 19th century when bacteria were classified in six genera placed in four tribes based on their morphological appearance. Since then the taxonomy of bacteria has been revolutionized several times. At present, 30 phyla belong to the domain “Bacteria”, which includes over 9600 species. Unlike many eukaryotes, bacteria lack complex morphological characters and practically phylogenetically informative fossils. It is partly due to these reasons that bacterial taxonomy is complicated. -
Influence of Rhizobia Inoculation on Biomass Gain and Tissue Nitrogen Content of Leucaena Leucocephala Seedlings Under Drought
Forests 2015, 6, 3686-3703; doi:10.3390/f6103686 OPEN ACCESS forests ISSN 1999-4907 www.mdpi.com/journal/forests Article Influence of Rhizobia Inoculation on Biomass Gain and Tissue Nitrogen Content of Leucaena leucocephala Seedlings under Drought Gabriela Pereyra 1,*, Henrik Hartmann 1, Beate Michalzik 2, Waldemar Ziegler 1 and Susan Trumbore 1 1 Max Planck Institute for Biogeochemistry, Hans-Knöll-str. 10, 07745 Jena, Germany; E-Mails: [email protected] (H.H.); [email protected] (W.Z.); [email protected] (S.T.) 2 Institute of Geography, Faculty of Chemical and Earth Sciences, Friedrich-Schiller-Universität Jena, Löbdergraben 32, 07743 Jena, Germany; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +49-3641-576176. Academic Editors: Reynaldo Campos Santana and Eric J. Jokela Received: 25 June 2015 / Accepted: 10 October 2015 / Published: 15 October 2015 Abstract: Anticipated increases in the frequency of heat waves and drought spells may have negative effects on the ability of leguminous trees to fix nitrogen (N). In seedlings of Leucaena leucocephala inoculated with Mesorhizobium loti or Rhizobium tropici, we investigated how the developmental stage and a short drought influenced overall biomass and the accumulation of carbon and N in plant tissues. In early developmental stages, the number of nodules and nodule biomass were correlated with total plant biomass and δ15N, and nodules and roots contributed 33%–35% of the seedling total N. Seedlings associated with R. tropici fixed more N and exhibited higher overall biomass compared with M. -
2010.-Hungria-MLI.Pdf
Mohammad Saghir Khan l Almas Zaidi Javed Musarrat Editors Microbes for Legume Improvement SpringerWienNewYork Editors Dr. Mohammad Saghir Khan Dr. Almas Zaidi Aligarh Muslim University Aligarh Muslim University Fac. Agricultural Sciences Fac. Agricultural Sciences Dept. Agricultural Microbiology Dept. Agricultural Microbiology 202002 Aligarh 202002 Aligarh India India [email protected] [email protected] Prof. Dr. Javed Musarrat Aligarh Muslim University Fac. Agricultural Sciences Dept. Agricultural Microbiology 202002 Aligarh India [email protected] This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically those of translation, reprinting, re-use of illustrations, broadcasting, reproduction by photocopying machines or similar means, and storage in data banks. Product Liability: The publisher can give no guarantee for all the information contained in this book. The use of registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. # 2010 Springer-Verlag/Wien Printed in Germany SpringerWienNewYork is a part of Springer Science+Business Media springer.at Typesetting: SPI, Pondicherry, India Printed on acid-free and chlorine-free bleached paper SPIN: 12711161 With 23 (partly coloured) Figures Library of Congress Control Number: 2010931546 ISBN 978-3-211-99752-9 e-ISBN 978-3-211-99753-6 DOI 10.1007/978-3-211-99753-6 SpringerWienNewYork Preface The farmer folks around the world are facing acute problems in providing plants with required nutrients due to inadequate supply of raw materials, poor storage quality, indiscriminate uses and unaffordable hike in the costs of synthetic chemical fertilizers. -
Reclassification of Rhizobium Tropici Type a Strains As Rhizobium Leucaenae Sp
International Journal of Systematic and Evolutionary Microbiology (2012), 62, 1179–1184 DOI 10.1099/ijs.0.032912-0 Reclassification of Rhizobium tropici type A strains as Rhizobium leucaenae sp. nov. Renan Augusto Ribeiro,1,23 Marco A. Rogel,33 Aline Lo´pez-Lo´pez,3 Ernesto Ormen˜o-Orrillo,1 Fernando Gomes Barcellos,4 Julio Martı´nez,3 Fabiano Lopes Thompson,5 Esperanza Martı´nez-Romero3 and Mariangela Hungria1 Correspondence 1Embrapa Soja, Cx. Postal 231, 86001-970, Londrina, Parana´, Brazil Ernesto Ormen˜o-Orrillo 2Universidade Estadual de Londrina, Department of Microbiology, Cx. Postal 60001, 86051-990, [email protected] Londrina, Parana´, Brazil 3Centro de Ciencias Geno´micas, Universidad Nacional Auto´noma de Me´xico, Cuernavaca, Morelos, Mexico 4Universidade Paranaense – UNIPAR, Cx. Postal 224, 87502-210, Umuarama, Parana´, Brazil 5UFRJ, Center of Health Sciences, Institute of Biology, Cx. Postal 68011, 21944-970, Rio de Janeiro, Brazil Rhizobium tropici is a well-studied legume symbiont characterized by high genetic stability of the symbiotic plasmid and tolerance to tropical environmental stresses such as high temperature and low soil pH. However, high phenetic and genetic variabilities among R. tropici strains have been largely reported, with two subgroups, designated type A and B, already defined within the species. A polyphasic study comprising multilocus sequence analysis, phenotypic and genotypic characterizations, including DNA–DNA hybridization, strongly supported the reclassification of R. tropici type A strains as a novel species. Type A strains formed a well-differentiated clade that grouped with R. tropici, Rhizobium multihospitium, Rhizobium miluonense, Rhizobium lusitanum and Rhizobium rhizogenes in the phylogenies of the 16S rRNA, recA, gltA, rpoA, glnII and rpoB genes. -
Characterization of New Exopolysaccharide Production by Rhizobium
International Journal of Biological Macromolecules 96 (2017) 361–369 Contents lists available at ScienceDirect International Journal of Biological Macromolecules journal homepage: www.elsevier.com/locate/ijbiomac Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate a,∗ a b Tereza Cristina Luque Castellane , João Carlos Campanharo , Luiz Alberto Colnago , b a a Isabel Duarte Coutinho , Érica Mendes Lopes , Manoel Victor Franco Lemos , a,∗ Eliana Gertrudes de Macedo Lemos a São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal b Embrapa Instrumentac¸ ão, Rua XV de Novembro 1452, São Carlos, São Paulo, 13560-970, Brazil a r t i c l e i n f o a b s t r a c t Article history: Exopolysaccharide (EPS) are produced by a diverse of rhizobia species and has been demonstrated to Received 28 June 2016 be a bioemulsifier with potential applications in the degradation of hydrocarbons. In the present study, Received in revised form attempts were made to obtain the new exopolysaccharide production by Rhizobium tropici (SEMIA 4080 23 November 2016 and MUTZC3) strains during growth on hydrocarbon substrate. Under the different cultivation condi- Accepted 29 November 2016 tions, the high molecular weight exopolysaccharides from Rhizobium tropici strains cultivated for 96 h Available online 20 December 2016 mainly consisted of carbohydrates (79–85%) and a low percentage of protein. The EPSC3-D differed from the others, with only 60% of carbohydrate. However, all strains produced polymers with distinct rheology Keywords: Bioemulsifier properties, such as viscosity of each EPS sample, suitable for different applications. In addition, RP- Exopolysaccharides HPLC, FTIR and NMR studies revealed EPS produced by rhizobia strains were similar indicating minimal Rhizobium tropici difference between EPS compositions. -
Türkiye'nin Eskişehir Ilinden, Phaseolus Vulgaris L.'Den Izole
www.biodicon.com Biological Diversity and Conservation ISSN 1308-8084 Online; ISSN 1308-5301 Print 11/2 (2018) 106-114 Research article/Araştırma makalesi Molecular identifications of rhizobial samples isolated from Phaseolus vulgaris L. in Eskişehir province of Turkey Cem Tolga GÜRKANLI *1, İbrahim ÖZKOÇ2, Merih KIVANÇ3 1 Ordu University, Fatsa Faculty of Marine Sciences, 52400, Fatsa, Ordu/Turkey 2Ondokuz Mayis University, Faculty of Arts and Science, Department of Biology, Atakum, Samsun/Turkey 3Anadolu University, Faculty of Science, Department of Biology, 26470, Eskişehir/Turkey Abstract In this study three rhizobial samples (R28, R51, R66) were anaysed using molecular phylogenetic methods. Rhizobial samples were isolated (from P. vulgaris L. root nodules collected from Eskişehir province of Turkey) and morphologically characterized in a previous study. Analyses were performed using a concatenated data set which composed of three housekeeping genes (recA, atpD and glnII). Additionally two symbiotic genes, nodA and nifH, were sequenced and analysed to determine the symbiotic plasmid type. As a result of housekeeping gene phylogeny, isolate R28 was found to be related to R. laguerreae. Although other two isolates, R51 and R66, placed in the same lineage with R. sophoriradicis, they showed enough divergence to be considered as a new species. But this presumption need to be confirmed with further investigations. Network analyses of nodA and nifH genes clearly showed that R28 has a unique symbiotic plasmid. On the other hand, symbiotic plasmids of R51 and R66 found to be related to p42d which is the most common symbiotic plasmid in rhizobia nodulating P. vulgaris L. in Europe and also Turkey. -
Evolution of Hiv and Its Vaccine
Journal of Current Perspectives in Applied Microbiology ISSN: 2278-1250 ©2012 Vol.1 (1) pp.1-5 JOURNAL OF CURRENT PERSPECTIVES IN APPLIED MICROBIOLOGY JULY 2012 VOLUME 1 NUMBER 1 ISSN: 2278-1250 CONTENTS S. Page Particulars No. No. 1 Viral Crusaders and Vaccine for HIV 3 P. Rajendran ………………………………………………………….. 2 Plant Growth Promoting Rhizobacteria for Biocontrol of 8 phytopathogens and Yield Enhancement of Phaseolus vulgaris L. Pankaj Kumar and R.C. Dubey…………..…………………………..... 3 Microbial Diversity in Coastal Mangrove Ecosystems 40 K. Kathiresan and R. Balagurunathan ………………………………... 4 Studies on Cadmium Biosorption by Cadmium Resistant 48 Bacterial Strains from Uppanar estuary, Cuddalore, Southeast Coast of India K. Mathivanan and R. Rajaram ……………………………………… 5 Isolation and Characterization of Plant Growth Promoting 54 Rhizobacteria (PGPR) from Vermistabilized Organic Substrates and their Effect on Plant Growth M. Prakash, V. Sathishkumar, T. Sivakami and N. Karmegam ……… 6 Isolation of Thermo Tolerant Escherichia coli from Drinking 63 Water of Namakkal District and Their Multiple Drug Resistance K. Srinivasan, C. Mohanasundari and K. Rasna .................................... 7 Isolation and Screening of Extremophilic Actinomycetes from 68 Alkaline Soil for the Biosynthesis of Silver Nanoparticles Vidhya and R. Balagurunathan ………………………………………. 8 BCL-2 Expression in Systemic Lupus Erythematosus Cases in 71 Chennai, Tamil Nadu M. Karthikeyan, P. Rajendran, S. Anandan, G. Ashok, S. Anbalagan, R. Nivedha,S. Melani Rajendran and Porkodi ………………………... 1 Journal of Current Perspectives in Applied Microbiology ISSN: 2278-1250 ©2012 Vol.1 (1) pp.1-5 July 2012, Volume 1, Number 1 ISSN: 2278-1250 Copy Right © JCPAM-2012 Vol. 1 No. 1 All rights reserved. No part of material protected by this copyright notice may be reproduced or utilized in any form or by any means, electronic or mechanical including photocopying, recording or by any information storage and retrieval system, without the prior written permission from the copyright owner. -
Compatibility Between Legumes and Rhizobia for the Establishment of a Successful Nitrogen-Fixing Symbiosis
G C A T T A C G G C A T genes Review Compatibility between Legumes and Rhizobia for the Establishment of a Successful Nitrogen-Fixing Symbiosis Joaquín Clúa, Carla Roda, María Eugenia Zanetti ID and Flavio A. Blanco * ID Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Centro Científico y Tecnológico-La Plata, Consejo Nacional de Investigaciones Científicas y Técnicas, 1900-La Plata, Argentina; [email protected] (J.C.); [email protected] (C.R.); [email protected] (M.E.Z.) * Correspondence: [email protected]; Tel.: +54-0221-4229-777 (ext. 33); Fax: +54-0221-4229-777 Received: 30 October 2017; Accepted: 8 February 2018; Published: 27 February 2018 Abstract: The root nodule symbiosis established between legumes and rhizobia is an exquisite biological interaction responsible for fixing a significant amount of nitrogen in terrestrial ecosystems. The success of this interaction depends on the recognition of the right partner by the plant within the richest microbial ecosystems on Earth, the soil. Recent metagenomic studies of the soil biome have revealed its complexity, which includes microorganisms that affect plant fitness and growth in a beneficial, harmful, or neutral manner. In this complex scenario, understanding the molecular mechanisms by which legumes recognize and discriminate rhizobia from pathogens, but also between distinct rhizobia species and strains that differ in their symbiotic performance, is a considerable challenge. In this work, we will review how plants are able to recognize and select symbiotic partners from a vast diversity of surrounding bacteria. -
Taxonomy of Rhizobia Frédéric Zakhia, Philippe De Lajudie
Taxonomy of rhizobia Frédéric Zakhia, Philippe de Lajudie To cite this version: Frédéric Zakhia, Philippe de Lajudie. Taxonomy of rhizobia. Agronomie, EDP Sciences, 2001, 21 (6-7), pp.569-576. 10.1051/agro:2001146. hal-00886134 HAL Id: hal-00886134 https://hal.archives-ouvertes.fr/hal-00886134 Submitted on 1 Jan 2001 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Agronomie 21 (2001) 569–576 569 © INRA, EDP Sciences, 2001 Review article Taxonomy of rhizobia Frédéric ZAKHIA, Philippe de LAJUDIE* Laboratoire des Symbioses Tropicales et Méditerranéennes, UMR 113 INRA/AGRO-M/CIRAD/IRD, TA 10 / J, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France (Received 15 January 2001; revised 8 March 2001; accepted 13 March 2001) Abstract – Rhizobia are the bacteria that form nitrogen-fixing symbioses with legumes. Based on their characterisation by polypha- sic taxonomy, their classification has undergone great changes in recent years. The current six rhizobium genera and 28 recognised species are reviewed here. Rhizobium / taxonomy Résumé – Taxonomie des rhizobia. Les rhizobia sont les bactéries qui forment des symbioses fixatrices d’azote avec des plantes de la famille des légumineuses. Suite à l’adoption de la taxonomie polyphasique comme critère de caractérisation, leur classification a subi de nombreux remaniements ces dernières années. -
Multi Locus Sequence Analysis of Root Nodule Isolates from Lotus
biblio.ugent.be The UGent Institutional Repository is the electronic archiving and dissemination platform for all UGent research publications. Ghent University has implemented a mandate stipulating that all academic publications of UGent researchers should be deposited and archived in this repository. Except for items where current copyright restrictions apply, these papers are available in Open Access. This item is the archived peer‐reviewed author‐version of: Multi Locus Sequence Analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov. C. Merabet, M. Martens, M. Mahdhi, F. Zakhia, A. Sy, C. Le Roux, O. Domergue, R. Coopman, A. Bekki, M. Mars, A.Willems and P. de Lajudie. In: International Journal of Systematic and Evolutionary Microbiology 60 (3), 664-674, 2010. http://ijs.sgmjournals.org/cgi/content/full/60/3/664 To refer to or to cite this work, please use the citation to the published version: Merabet C., M. Martens, M. Mahdhi, F. Zakhia, A. Sy, C. Le Roux, O. Domergue, R. Coopman, A. Bekki, M. Mars, A.Willems and P. de Lajudie. 2010. Int. J. Syst. Evol. Microbiol. 60:664-674. DOI 10.1099/ijs.0.012088-0 1 IJS/2009/012088 REVISED MS 2 3 Multi Locus Sequence Analysis of root nodule isolates from Lotus arabicus 4 (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa 5 (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer 6 garamanticus sp. nov. 7 8 C. Merabet1,2, M. Martens3, M. Mahdhi2,4, F.