Anti-PPM1B Antibody [K1b1] (ARG56955)

Product datasheet [email protected]

ARG56955 Package: 50 μl anti-PPM1B antibody [k1B1] Store at: -20°C

Summary

Product Description Mouse Monoclonal antibody [k1B1] recognizes PPM1B

Tested Reactivity Hu

Tested Application FACS, WB

Host Mouse

Clonality Monoclonal

Clone k1B1

Isotype IgG2a, kappa

Target Name PPM1B

Antigen Species Human

Immunogen Recombinant fragment around aa. 1-192 of Human PPM1B.

Conjugation Un-conjugated

Alternate Names Protein phosphatase 1B; PP2CBETA; PP2C-beta-X; PPC2BETAX; PP2C-beta; PP2CB; EC 3.1.3.16; Protein phosphatase 2C isoform beta

Application Instructions

Application table Application Dilution

FACS Assay-dependent

WB 1:1000 - 1:3000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Calculated Mw 53 kDa

Properties

Form Liquid

Purification Purification with Protein G.

Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.

Preservative 0.02% Sodium azide

Stabilizer 10% Glycerol

Concentration 1 mg/ml

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/3 Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links GeneID: 5495 Human

Swiss-port # O75688 Human

Gene Symbol PPM1B

Gene Full Name protein phosphatase, Mg2+/Mn2+ dependent, 1B

Background The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. [provided by RefSeq, Jul 2008]

Function Enzyme with a broad specificity. Dephosphorylates CDK2 and CDK6 in vitro. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB. [UniProt]

Images

ARG56955 anti-PPM1B antibody [k1B1] FACS image

Flow Cytometry: A549 cell line stained with ARG56955 anti-PPM1B antibody [k1B1] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.

ARG56955 anti-PPM1B antibody [k1B1] WB image

Western blot: 30 µg of MCF7 cell lysate stained with ARG56955 anti- PPM1B antibody [k1B1] at 1:2000.

www.arigobio.com 2/3 ARG56955 anti-PPM1B antibody [k1B1] FACS image

Flow Cytometry: HeLa cell line stained with ARG56955 anti-PPM1B antibody [k1B1] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.

ARG56955 anti-PPM1B antibody [k1B1] WB image

Western blot: 40 µg of A431 cell lysate stained with ARG56955 anti- PPM1B antibody [k1B1] at 1:1000.