Anti-MAML1 (Aa 655-754) Polyclonal Antibody (DPAB-DC3896) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use

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Anti-MAML1 (aa 655-754) polyclonal antibody (DPAB-DC3896) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Antigen Description This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. Immunogen MAML1 (NP_055572, 655 a.a. ~ 754 a.a) partial recombinant protein with GST tag. The sequence is AEQEKQQFQRHLTRPPPQYQDPTQGSFPQQVGQFTGSSAAVPGMNTLGPSNSSCPRVFPQA GNLMPMGPGHASVSSLPTNSGQQDRGVAQFPGSQNMPQS Source/Host Mouse Species Reactivity Human Conjugate Unconjugated Applications WB (Recombinant protein), ELISA, Size 50 μl Buffer 50 % glycerol Preservative None Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. GENE INFORMATION Gene Name MAML1 mastermind-like 1 (Drosophila) [ Homo sapiens (human) ] Official Symbol MAML1 Synonyms MAML1; mastermind-like 1 (Drosophila); Mam1; Mam-1; mastermind-like protein 1; mastermind homolog; 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Entrez Gene ID 9794 Protein Refseq NP_055572 UniProt ID Q92585 Chromosome Location 5q35 Pathway Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants; Delta-Notch Signaling Pathway; FBXW7 Mutants and NOTCH1 in Cancer; Generic Transcription Pathway Function peptide antigen binding; protein binding; protein kinase binding; transcription coactivator activity 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.

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MAML1 Antibody A
Revision 1 C 0 2 - t MAML1 Antibody a e r o t S Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) 8 0 Web: [email protected] 6 www.cellsignal.com 4 # 3 Trask Lane Danvers Massachusetts 01923 USA For Research Use Only. Not For Use In Diagnostic Procedures. Applications: Reactivity: Sensitivity: MW (kDa): Source: UniProt ID: Entrez-Gene Id: WB, IP H Endogenous 130 Rabbit Q92585 9794 Product Usage Information Application Dilution Western Blotting 1:1000 Immunoprecipitation 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. Specificity / Sensitivity MAML1 Antibody detects endogenous levels of total MAML1 protein. It does not cross- react with MAML2 and MAML3. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His811 of human MAML1. Antibodies are purified by protein A and peptide affinity chromatography. Background Mastermind-like (MAML) family of proteins are homologs of Drosophila Mastermind. The family is composed of three members in mammals: MAML1, MAML2, and MAML3 (1,2). MAML proteins form complexes with the intracellular domain of Notch (ICN) and the transcription factor CSL (RBP-Jκ) to regulate Notch target gene expression (3-5). MAML1 also interacts with myocyte enhancer factor 2C (MEF2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthin's tumors (7).
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Cystatin-B Negatively Regulates the Malignant Characteristics of Oral Squamous Cell Carcinoma Possibly Via the Epithelium Proliferation/ Differentiation Program
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International Journal of Molecular Sciences Article Selenite Inhibits Notch Signaling in Cells and Mice Michael Powers 1, Liu Liu 1, Dane Deemer 1, Selina Chen 1, Aaron Scholl 1, Masafumi Yoshinaga 2 and Zijuan Liu 1,* 1 Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA; [email protected] (M.P.); [email protected] (L.L.); [email protected] (D.D.); [email protected] (S.C.); [email protected] (A.S.) 2 Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL 33199, USA; myoshina@fiu.edu * Correspondence: [email protected]; Tel.: +1-248-370-3554 Abstract: Selenium is an essential micronutrient with a wide range of biological effects in mammals. The inorganic form of selenium, selenite, is supplemented to relieve individuals with selenium deficiency and to alleviate associated symptoms. Additionally, physiological and supranutritional selenite have shown selectively higher affinity and toxicity towards cancer cells, highlighting their potential to serve as chemotherapeutic agents or adjuvants. At varying doses, selenite extensively regulates cellular signaling and modulates many cellular processes. In this study, we report the identification of Delta–Notch signaling as a previously uncharacterized selenite inhibited target. Our transcriptomic results in selenite treated primary mouse hepatocytes revealed that the tran- scription of Notch1, Notch2, Hes1, Maml1, Furin and c-Myc were all decreased following selenite treatment. We further showed that selenite can inhibit Notch1 expression in cultured MCF7 breast adenocarcinoma cells and HEPG2 liver carcinoma cells. In mice acutely treated with 2.5 mg/kg Citation: Powers, M.; Liu, L.; selenite via intraperitoneal injection, we found that Notch1 expression was drastically lowered in Deemer, D.; Chen, S.; Scholl, A.; liver and kidney tissues by 90% and 70%, respectively.
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Computational Approach to Identify Mutations in Genes of Notch Signaling Pathway and Its Association with OSCC
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Supporting Information
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