RESEARCH ARTICLE A Comprehensive Assessment of the Effects of Transgenic Cry1Ac/Cry1Ab Rice Huahui 1 on Adult Micraspis discolor (Fabricius) (Coleoptera: Coccinellidae) Xia Zhou, Yunling Guo, Hua Kong, Jiao Zuo, Qixing Huang, Ruizong Jia, Anping Guo*, Lin Xu Key laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of a11111 Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Science, Haikou, Hainan, 571101, P.R. China *
[email protected] Abstract OPEN ACCESS Micraspis discolor (Fabricius) (Coleoptera: Coccinellidae) is a widely distributed coleoptera Citation: Zhou X, Guo Y, Kong H, Zuo J, Huang Q, predator in southern Asia in rice ecosystem, and adult M. discolor feed on both rice pollen Jia R, et al. (2016) A Comprehensive Assessment of and soft-bodied arthropods. Bitrophic bioassay and tritrophic bioassay were conducted to the Effects of Transgenic Cry1Ac/Cry1Ab Rice Huahui 1 on Adult Micraspis discolor (Fabricius) evaluate the potential impact of Cry1Ac/Cry1Ab-expressing rice Huahui 1 and its non- (Coleoptera: Coccinellidae). PLoS ONE 11(2): transgenic counterpart Minghui 63 on fitness parameters of adult M. discolor. The results e0142714. doi:10.1371/journal.pone.0142714 showed that the survival, and fecundity of this beetle’ adults were not different when they Editor: Mario Soberón, Instituto de Biotecnología, fed on Bt rice or non-Bt rice pollen or Nilaparvata lugens (Stål) reared on Bt rice or non-Bt Universidad Nacional Autónoma de México, MEXICO rice. Toxicity assessment to ensure M. discolor adults were not sensitive to Cry1Ab or Received: March 27, 2015 Cry1Ac protein independent from the pollen background, M.