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201700153.Pdf Continuation Page from ST – 470 (Application for Plant Variety Protection Certificate) 22. CONTINUED FROM FRONT (Please provide a statement as to the limitation and sequence of generations that may be certified.) 201700153 23. CONTINUED FROM FRONT (Please provide the date of first sale, disposition, transfer, or use for each country and the circumstances, if the variety (including any harvested material) or a hybrid produced from this variety has been sold, disposed of, transferred, or used in the U.S. or other countries.) 24. CONTINUED FROM FRONT (Please give the country, date of filing or issuance, and assigned reference number, if the variety or any component of the variety is protected by intellectual property right (Plant Breeder's Right or Patent).) U.S. Patent Application date: March 31, 2016 - Serial No. 15/086545 ST - 470 (2012) designed by the Plant Variety Protection Office Page 7 of 10 CLARIFICATION OF DATA IN EXHIBITS B AND C Please note the data presented in Exhibit B and C, "Objective Description of Variety," are collected in Johnston, Iowa. The quantitative data presented in Exhibit B are from two sample t-tests using data collected during the two growing 201700153 seasons shown, 2015 and 2016. Qualitative trait data are presented from environments where the data best represents the variety(ies). The traits in Exhibit B collectively show distinct differences between the two varieties. For each given year of data collection, our experimental design was arranged so entries with similar maturities were planted side by side with one replication of the new variety grown in each environmental location. The experiment procedures are carried out over a two year period with observation plots planted in 17.42 ft., 4 row plots for each variety. Approximately 30 plants emerge per row and are thinned for a total of 24 too 26 plants in each of the 4 rows for a total of approximately 96 to 104 plants being evaluated in each environment and a minimum of 192 to 208 plants across multiple years. For plant level traits, we sampled up to 20 representative plants from a single row of the 4 row plot (group) of plants. For plot level traits we evaluated the 4 row plot (group) and observed a representative score or average on the 96-104 plants in the group within an experiment. Growing Degree Precipitation Growing Degree Precipitation Units (GDUs) (Inches) Units (GDUs) (Inches) 2015 2015 2016 2016 Month Johnston, IA Johnston, IA Johnston, IA Johnston, IA May 429 4.78 402 2.86 June 647 8 719 3.14 July 748 8.62 729 8.38 August 650 3.71 709 6.88 September 623 7.61 574 4.01 Total 3097 33.83 3133 25.27 Growing Degree Units use following formula: GDU = ((T1+T2)/2)-50 Where T1 = minimum temperature for a given day with 50 degrees Fahrenheit as the minimum temperature used and 86 degrees Fahrenheit is the maximum temperature used. Where T2 = maximum temperature for a given day with 86 degrees Fahrenheit as the maximum temperature used and 50 degrees Fahrenheit is the minimum temperature used. GDUs are calculated each day and accumulated (summed) over certain number of days. ST - 470 (2012) designed by the Plant Variety Protection Office Exhibit C (Corn) 9. COB: Standard Deviation Sample Size __20.3 __ __ mm Cob Diameter at mid-point __________0.73 ________19 __ __1 Cob Color (Munsell Code __________________________________)2.5Y9/2 1= White (ex. Munsell 5Y 9/1 or 2.5Y 8.5/2 ) 2= Pink (ex, Munsell 2.5R 7/6 or 5R 5/6 ) 3= Red (ex. Munsell 2.5R 4/8 or 10RP 4/8 ) 4= Other (descr be ) 201700153 10. DISEASE RESISTANCE (Rate from 1 (most susceptible) to 9 (most resistant); leave blank if not tested; leave Race or Strain Options blank if polygenic): A. Leaf Blights, Wilts, and Local Infection Diseases ___ Anthracnose Leaf Blight (Colletotrichum graminicola) ___ Common Rust (Puccinia sorghi) ___ Common Smut (Ustilago maydis) ___ Eyespot (Kabatiella zeae) ___ Goss’s Wilt (Clavibacter michiganense spp. nebraskense) ___ Gray Leaf Spot (Cercospora zeae-maydis) ___ Helminthosporium Leaf Spot (Bipolaris zeicola) Race __________ ___ Northern Leaf Blight (Exserohilum turcicum) Race __________ ___ Southern Leaf Blight (Bipolaris maydis) Race __________ ___ Southern Rust (Puccinia polysora) ___ Stewart’s Wilt (Erwinia stewartii) ___ Other (Specify) ________________________________________________ B. Systemic Diseases ___ Corn Lethal Necrosis (MCMV and MDMV) ___ Head Smut (Sphacelotheca reiliana) ___ Maize Chlorotic Dwarf Virus (MCDV) ___ Maize Chlorotic Mottle Virus (MCMV) ___ Maize Dwarf Mosaic Virus (MDMV) Strain ______________ ___ Sorghum Downy Mildew of Corn (Peronosclerospora sorghi) ___ Other (Specify) ________________________________________________ C. Sta k Rots ___ Anthracnose Stalk Rot (Colletotrichum graminicola) ___ Diplodia Stalk Rot (Stenocarpella maydis) ___ Fusarium Sta k Rot (Fusarium moniliforme) ___ Gibberella Sta k Rot (Gibberella zeae) ___ Other (Specify) ________________________________________________ D. Ear and Kernel Rots ___ Aspergillus Ear and Kernel Rot (Aspergillus flavus) ___ Diplodia Ear Rot (Stenocarpella maydis) ___ Fusarium Ear and Kernel Rot (Fusarium moniliforme) ___ Gibberella Ear Rot (Gibberella zeae) ___ Other (Specify) ____________________________________ 11. INSECT RESISTANCE (Rate from 1 (most susceptible) to 9 (most resistant) Leave blank if not tested): Standard Deviation Sample Size __ Banks Grass Mite (Oligonychus pratensis) Corn Earworm (Helicoverpa zea) __ Leaf-Feeding Silk Feeding: __ __ __·__ mg larval wt. ____________ ________ __ Ear Damage __ Corn Leaf Aphid (Rhopalosiphum maidis) __ Corn Sap Beetle (Carpophilus dimidiatus) ST-470-28 (06/2015) designed by the Plant Variety Protection Office Page 6 of 8 Exhibit C (Corn) 11. INSECT RESISTANCE (continued) Standard Deviation Sample Size Fall Armyworm (Spodoptera frugiperda) __ Leaf-Feeding Silk Feeding: __ __ __·__ mg larval wt. ____________ ________ __ Maize Weevil (Sitophilus zeamaize) 201700153 __ Northern Rootworm (Diabrotica barberi) __ Southern Rootworm (Diabrotica undecimpunctata) Southwestern Corn Borer (Diatraea grandiosella) __ Leaf-Feeding Stalk Tunneling: __ __ __·__ cm tunneled/plant ____________ ________ __ Two-spotted Spider Mite (Tetranychus urticae) __ Western Rootworm (Diabrotica virgifera virgifera) __ Other (Specify) ____________________________________ 12. AGRONOMIC TRAITS: __ Stay Green (at 65 days after anthesis) (Rate on a scale of 1 = worst to 9 = excellent) __ __·__ % Dropped Ears (at 65 days after anthesis) __ __·__ % Pre-anthesis Brittle Snapping __ __·__ % Pre-anthesis Root Lodging __ __·__ % Post-anthesis Root Lodging (at 65 days after anthesis) __ __ __·__ Kg/ha Yield of Inbred Per Se (at 12-13% grain moisture) 13. MOLECULAR MARKERS: (0 = data unavailable; 1 = data available but not supplied; 2 = data supplied) __ Isozymes __ RFLP’s __ RAPD’s SSRs SNPs __ Other (Specify) _________________________________________________ Characteristics of Isozyme Polymorphism: 1= Absent 2= Present ___ Acp1 ___ Acp4 ___ Adh1 ___ Dia1 ___ Dia2 ___ Idh1 ___ Idh2 ___ Mdh1 ___ Mdh2 ___ Mdh3 ___ Mmm ___ Mdh4 ___ Mdh5 ___ Mdh6 ___ Pgd1 ___ Pgd2 ___ Pgi1 ___ Pgm1 ___ Pgm2 ___ Phi1 ST-470-28 (06/2015) designed by the Plant Variety Protection Office Page 7 of 8 Exhibit C (Corn) REFERENCES: Butler, D.R. 1954. A System for the Classification of Corn Inbred Lines. PhD Thesis. Ohio State University. Emerson, R.A., G.W. Beadle, and A.C. Fraser. 1935. A Summary of Linkage Studies in Maize. Cornell A.E.S., Mem. 180. Farr, D. F., G. F. Bills, G.P. Chamuris, A.Y. Rossman. 1989. Fungi on Plant and Plant Products in the United States. The American Phytopathological Society. St. Paul, MN. Inglett, G. E. (Ed.) 1970. Corn: Culture, Processing, Products. Avi Publishing Company, Westport, CT. Jugenheimer, R. W. 1976. Corn: Improvement, Seed Production, and Uses. John Wiley & Sons, New York. 201700153 McGee, D.C. 1988. Maize Diseases. APS Press. St. Paul, MN. 150 pp. Munsell Color Chart for Plant Tissues. Macbeth. P.O. Box 230, Newburgh, NY 12551-0230. The Mutants of Maize. 1968. Crop Science Society of America, Madison, WI. Shurtleff, M.C. 1980. Compendium of Corn Diseases. APS Press. St. Paul, MN. 105 pp. Sprague, G.F., and J.W. Dudley (Editors). 1988. Corn and Corn Improvement. Third Edition. Agronomy Monograph 18. ASA, CSSA, SSSA, Madison, WI. Stringfield, G.H. Maize Inbred Lines of Ohio. Ohio A.E.S., Bul. 831. 1959. U. S. Department of Agriculture. 1936. 1937. Yearbook. COMMENTS: (e.g., state how heat units were calculated, standard inbred seed source, and/or where data was collected. Continue in Exhibit D.) ST-470-28 (06/2015) designed by the Plant Variety Protection Office Page 8 of 8 .
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