A peer-reviewed open-access journal ZooKeys 445: 57–76 (2014) A new quadrannulate species of Orobdella ... 57 doi: 10.3897/zookeys.445.7999 RESEARCH ARTICLE http://zookeys.pensoft.net Launched to accelerate biodiversity research A new quadrannulate species of Orobdella (Hirudinida, Arhynchobdellida, Orobdellidae) from central Honshu, Japan Takafumi Nakano1 1 Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan Corresponding author: Takafumi Nakano (
[email protected]) Academic editor: Fredric Govedich | Received 31 May 2014 | Accepted 05 September 2014 | Published 13 October 2014 http://zoobank.org/E3A379D5-EF2B-4378-BA88-6C662EF1BEA7 Citation: Nakano T (2014) A new quadrannulate species of Orobdella (Hirudinida, Arhynchobdellida, Orobdellidae) from central Honshu, Japan. ZooKeys 445: 57–76. doi: 10.3897/zookeys.445.7999 Abstract A new quadrannulate species of Orobdella, Orobdella masaakikuroiwai sp. n., from the mountainous re- gion of central Honshu, Japan is described. This is only the second small species known within this genus, with a body length of less than 4 cm for mature individuals. Phylogenetic analyses using nuclear 18S rDNA and histone H3 as well as mitochondrial COI, tRNACys, tRNAMet, 12S, tRNAVal, 16S, and ND1 markers showed that O. masaakikuroiwai sp. n. is the sister species of the quadrannulate O. whitmani Oka, 1895. Phylogenetic relationships within O. masaakikuroiwai sp. n. conducted using mitochondrial markers reveled a distinction between eastern and western phylogroups. Keywords Hirudinea, Hirudinida, Orobdella, new species, gastroporous, molecular phylogeny, Japan Introduction The genus Orobdella Oka, 1895 is an East Asian terrestrial macrophagous leech taxon assigned to the family Gastrostomobdellidae Richardson, 1971, along with the South- east Asian terrestrial macrophagous genus Gastrostomobdella Moore, 1929 (Richardson 1971).