Supplemental Table 17: Sulfhydrated Protein Pathway Enrichment in CGL KO Liver KEGG Pathway (DR) p‐val (adj) ‐LOG10(p‐val adj) Involved Gene No KEGG

KEGG Pathway (Unchanged) p‐val (adj) ‐LOG10(p‐val adj) Involved Gene Vitamin B6 metabolism 0.0442 1.35 PNPO,PDXK,AOX1,AOX3 Peroxisome 1.26E‐07 6.90 SOD2,ACAA1B,PIPOX,ACOX1,HACL1,EPHX2,SOD1,XDH,HSD17B4,PRDX5,IDH1,AGXT,CAT,HAO1,SCP2,HMGCL,PRDX1,NUDT7,ACAA1A,ECH1 Proteasome 5.84E‐09 8.23 PSMD4,PSMB4,PSMB1,PSMA2,PSMB6,PSMA6,PSMB5,PSME1,PSMB8,PSMB7,PSMA7,PSMB2,PSMA1,PSMA4,PSMA5,PSMB3 Arginine and proline metabolism 0.00186 2.73 ARG1,CNDP2,GOT1,ALDH9A1,ALDH4A1,ALDH2,OAT,GOT2,LAP3,AGMAT,ALDH7A1 Histidine metabolism 0.0000753 4.12 FTCD,AMDHD1,HAL,ASPA,CNDP2,ALDH9A1,ALDH2,UROC1,ALDH7A1 DLST,SDHB,PGAM1,MDH2,MDH1,OGDH,SHMT1,DLD,ALDH6A1,SDHA,GLUD1,TKT,ESD,ACO2,GPT,TPI1,ACAT2,MUT,GLDC,GOT1,ECHS1,TALDO1,IDH1,CPS1,AGXT,CAT,HAO1,ALDOB,PRPS1,GOT2,ACAT1,PCCB,TKFC,PKLR,PGP,GAPDH,PGK Carbon metabolism 8.76E‐21 20.06 1,ENO1 2‐Oxocarboxylic acid metabolism 0.0136 1.87 ACO2,GPT,ACY1,GOT1,IDH1,GOT2 Selenocompound metabolism 0.00964 2.02 TXNRD1,SCLY,SEPHS1,SEPSECS,KYAT1,SEPHS2 Cysteine and methionine metabolism 0.0000013 5.89 APIP,MDH2,MDH1,CBS,GOT1,AHCY,GSS,GCLM,GOT2,MAT1A,MAT2B,BHMT2,LDHA,BHMT Butanoate metabolism 0.00016 3.80 ACAT2,ECHS1,HMGCS2,HADH,BDH2,HMGCL,ACAT1,ALDH5A1,ABAT Propanoate metabolism 0.000585 3.23 DLD,ALDH6A1,ACAT2,MUT,ECHS1,ACAT1,PCCB,ABAT,LDHA Glycine, serine and threonine metabolism 0.00000196 5.71 GNMT,PGAM1,CHDH,PIPOX,SHMT1,DLD,CBS,GLDC,AGXT,GRHPR,DMGDH,ALDH7A1,BHMT Pyruvate metabolism 3.94E‐08 7.40 MDH2,MDH1,DLD,ACAT2,GLO1,HAGH,ALDH9A1,ALDH2,LDHD,ACAT1,GRHPR,PKLR,ALDH7A1,LDHA Tryptophan metabolism 0.00000051 6.29 HAAO,GCDH,OGDH,ACAT2,ECHS1,ALDH9A1,CAT,HADH,ALDH2,ACAT1,KYAT1,ALDH7A1,AOX1,AOX3 Valine, leucine and isoleucine degradation 4.06E‐14 13.39 ACAA1B,DLD,ALDH6A1,AUH,MCCC2,ACAT2,MUT,ECHS1,ALDH9A1,HMGCS2,HADH,HMGCL,ALDH2,HIBADH,ACAT1,PCCB,ACAA1A,ACAA2,ALDH7A1,ABAT,AOX1,AOX3 Drug metabolism ‐ other enzymes 1.56E‐07 6.81 GSTM3,MGST1,MPO,NME2,DPYS,XDH,GSTO1,HPRT1,GSTA3,CMPK1,CES1F,CES2E,DPYD,UPB1,NME1,GSTM2,CES1D,GSTM1,IMPDH2,NME3 Fatty acid degradation 2.51E‐07 6.60 GCDH,ACAA1B,ACOX1,ACAT2,ECI1,ECHS1,ACADL,ALDH9A1,HADH,ALDH2,ACAT1,ACAA1A,ACAA2,ALDH7A1,ADH1 Fatty acid metabolism 0.0464 1.33 ACAA1B,ACOX1,ACAT2,ECHS1,ACADL,HADH,ACAT1,ACAA1A,ACAA2 Glyoxylate and dicarboxylate metabolism 3.94E‐11 10.40 MDH2,MDH1,SHMT1,DLD,ACO2,ACAT2,MUT,GLDC,AGXT,CAT,HAO1,ACAT1,PCCB,GRHPR,PGP Glutathione metabolism 0.00318 2.50 GSTM3,MGST1,GSTO1,GSTA3,IDH1,GSS,GCLM,GSR,LAP3,GSTM2,GSTM1,GPX1 Arginine biosynthesis 1.97E‐07 6.71 ARG1,GLUD1,GPT,ACY1,GOT1,ASL,CPS1,OTC,GOT2,ASS1 Alanine, aspartate and glutamate metabolism 2.27E‐07 6.64 ASPA,GLUD1,GPT,NIT2,GOT1,ASL,CPS1,AGXT,ALDH4A1,GOT2,ALDH5A1,ABAT,ASS1 Tyrosine metabolism 0.00555 2.26 GSTZ1,HGD,GOT1,HPD,FAH,GOT2,AOX1,AOX3,ADH1 Synthesis and degradation of ketone bodies 0.00686 2.16 ACAT2,HMGCS2,BDH2,HMGCL,ACAT1 Biosynthesis of amino acids 1.75E‐13 12.76 PGAM1,ARG1,SHMT1,TKT,ACO2,GPT,ACY1,TPI1,CBS,GOT1,TALDO1,ASL,IDH1,CPS1,ALDOB,OTC,PRPS1,GOT2,MAT1A,PKLR,MAT2B,GAPDH,PGK1,ENO1,ASS1 beta‐Alanine metabolism 0.000112 3.95 ALDH6A1,DPYS,CNDP2,ECHS1,ALDH9A1,ALDH2,DPYD,UPB1,ALDH7A1,ABAT Glycolysis / Gluconeogenesis 0.0000237 4.63 PGAM1,DLD,TPI1,ALDH9A1,ALDOB,PGM1,ALDH2,GALM,PKLR,ALDH7A1,GAPDH,PGK1,LDHA,ENO1,ADH1 Lysine degradation 0.000796 3.10 GCDH,DLST,PIPOX,OGDH,ACAT2,ECHS1,ALDH9A1,HADH,ALDH2,ACAT1,BBOX1,ALDH7A1

HAAO,FTCD,PAFAH1B2,GCDH,DLST,SDHB,ACAA1B,APIP,PGAM1,QDPR,AMDHD1,CHDH,PIPOX,PNPO,MDH2,ETNPPL,ARG1,HAL,PCBD1,MDH1,OGDH,SHMT1,DLD,ASPA,ACOX1,NME2,GSTZ1,PYGL,ALDH6A1,AUH,SDHA,NDUFS6,MCCC2,G LUD1,TKT,EPHX2,CMBL,DPYS,ACO2,GPT,CPOX,HGD,ACY1,TPI1,ACAT2,MUT,CBS,XDH,NDUFV2,HSD17B4,CNDP2,GLDC,NT5C2,PCYT2,GOT1,ECHS1,URAH,TALDO1,ASL,HPRT1,IDH1,NDUFS1,CPS1,ACADL,AGXT,SCLY,BPNT1,SEPHS1,ALDH9 A1,PRDX6,SORD,HAO1,IMPA1,AHCY,GSS,HMGCS2,HADH,GCLM,BDH2,UOX,ALDOB,ALAD,SCP2,HMGCL,CMPK1,ALDH4A1,AK2,KHK,PGM1,UGDH,PAICS,HPD,ALDH2,HIBADH,FAH,QPRT,OAT,OTC,PRPS1,GOT2,CES1F,ACAT1,PCCB,PDXK,DP YD,UPB1,TKFC,UROC1,ATP5H,GALM,GRHPR,ALDH5A1,GALT,ACAA1A,ACAA2,GCH1,NME1,MAT1A,NDUFV1,UQCRFS1,ADK,KYAT1,LAP3,BLVRB,AGMAT,PKLR,MAT2B,DMGDH,BHMT2,PGP,ACP1,GPHN,SEPHS2,SUOX,ALDH1A1,ALDH7A1, Metabolic pathways 3.31E‐31 30.48 CES1D,GAPDH,ABAT,GDA,PGK1,IMPDH2,LDHA,ENO1,AOX1,PPCDC,MOCS1,AOX3,PTGES3,GANAB,NME3,ADH1,BHMT,URAD,ASS1 Citrate cycle (TCA cycle) 0.000782 3.11 DLST,SDHB,MDH2,MDH1,OGDH,DLD,SDHA,ACO2,IDH1

KEGG Pathway (AL) p‐val (adj) ‐LOG10(p‐val adj) Involved Gene No KEGG