Risk Assessment and Recommended Disease Screening
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A Manual For Control of Infectious Diseases in Amphibian Survival Assurance Colonies and Reintroduction Programs Version 2.0: updated 2017 Based on: Proceedings from a Workshop: 16–18 February 2009 San Diego Zoo Cover photos courtesy of Allan Pessier and Ron Holt. A contribution of the IUCN/SSC Conservation Breeding Specialist Group in collaboration with Amphibian Ark, San Diego Zoo, and Zoo Atlanta IUCN encourages meetings, workshops and other fora for the consideration and analysis of issues related to conservation, and believes that reports of these meetings are most useful when broadly disseminated. The opinions and views expressed by the authors may not necessarily reflect the formal policies of IUCN, its Commissions, its Secretariat or its members. © Copyright CBSG 2017 The designation of geographical entities in this book, and the presentation of the material, do not imply the expression of any opinion whatsoever on the part of IUCN concerning the legal status of any country, territory, or area, or of its authorities, or concerning the delimitation of its frontiers or boundaries. Pessier, A.P. and J.R. Mendelson III (eds.). 2017. A Manual for Control of Infectious Diseases in Amphibian Survival Assurance Colonies and Reintroduction Programs, Ver. 2.0. IUCN/SSC Conservation Breeding Specialist Group: Apple Valley, MN. An electronic version of this report can be downloaded at www.cbsg.org <http://www.cbsg.org/> . This project was supported by grant LG-25-08-0066 from the Institute of Museum and Library Services. Any views, findings, conclusions or recommendations expressed in this publication do not necessarily represent those of the Institute of Museum and Library Services. A MANUAL FOR CONTROL OF INFECTIOUS DISEASES IN AMPHIBIAN SURVIVAL ASSURANCE COLONIES AND REINTRODUCTION PROGRAMS EDITORS: ALLAN P. PESSIER & JOSEPH R. MENDELSON III PROCEEDINGS FROM A WORKSHOP: 16–18 FEBRUARY 2009 Zoological Society of San Diego Organized by: Allan P. Pessier, DVM, Dipl. ACVP San Diego Zoo & Joseph R. Mendelson III, PhD Zoo Atlanta Facilitated by: Onnie Byers, PhD IUCN Conservation Breeding Specialist Group Updated March 2017 by: Allan P. Pessier, DVM, Dipl. ACVP San Diego Zoo & Joseph R. Mendelson III, PhD Zoo Atlanta Benjamin Tapley, MSc ZSL London Zoo Matt Goetz Durrell Wildlife Conservation Trust Table of Contents 1. INTRODUCTION………………………………………………………….………………………………….……….. 8 2. LIST OF CONTRIBUTORS………………………………………………….………………………….….……….. 10 3. RISK ASSESSMENT………………………………………………………….….……………………….…………. 11 3.0 INTRODUCTION…………………………………………….………………..………….………….. 11 3.1 GENERAL RECOMMENDATIONS FOR REINTRODUCTION AND TRANSLOCATION PROGRAMS………………………… .………………………….………… 11 3.2 DISEASE RISK MITIGATION FOR CAPTIVE BREEDING AND REINTRODUCTION PROGRAMS…………………………….………………………………………..….………….. 13 3.3 TRANSLOCATION……………………………………….…………………………….……………. 19 3.4 REFERENCES……………………………………..…………………………………….…………… 20 4. BIOSECURITY………………………………………………………………………………………….……………. 21 4.0 INTRODUCTION……………………………………………………..………………….…………… 21 4.1 WHAT IS "BIOSECURITY"?............................................................................. 22 4.2 BIOSECURITY DEFINITIONS…………………………………… ………….…………….….…… 23 4.3 COSMOPOLITAN COLLECTIONS AND RISKS OF DISEASE FOR WILD AMPHIBIANS… ... 23 4.4 LEVELS OF BIOSECURITY…………………………………………………………………..…..… 24 4.5 SUMMARY OF BEST PRACTICES AND ISOLATION SECURITY LEVELS……………….….... 30 4.6 BIOSECURITY PRACTICES……………………………………………………………………….… 33 4.7 STAFF TRAINING AND IMPLEMENTATION OF BIOSECURITY PRACTICES………………… 35 4.8 LONG-TERM ISOLATION OF AMPHIBIANS DESTINED FOR REINTRODUCTION TO THE WILD……………………………………………………………….…………….…….. 35 4.9 FACILITIES FOR LONG-TERM ISOLATION……………………………………………….….…. 36 4.10 HUSBANDRY STAFF HYGIENE AND PROTECTIVE CLOTHING……………………….….…. 37 4.11 HUSBANDRY ROUTINES……………………………………………………………………….... 39 4.12 ENCLOSURE SANITATION……………………………………………………………………...… 41 4.13 EQUIPMENT, ENCLOSURE SUBSTRATES AND DECORATION…………………….….....… 41 4.14 WASTEWATER DISPOSAL…………………………………………………………………..….… 42 4.15 SOLID WASTE DISPOSAL……………………………………………………………….………… 44 4.16 SOURCES OF WATER………………………………………………………………………….….. 45 4.17 SOURCES OF FOOD……………………………………………………………………………...… 45 4.18 FACILITY DESIGN AND BUILDING SPECIFICATIONS………………………………….………. 46 4.19 REFERENCES……………………………………………………………………………….……..… 48 5. CLEANING AND DISINFECTION…………………………………………………………………..……………. 50 5.0 INTRODUCTION…………………………………………………………………………………….… 50 5.1 PRINCIPLES OF CLEANING & DISINFECTION………………………………..……………….…. 51 5.2 CHEMICAL DISINFECTANTS………………………………………………………..…………….… 53 5.3 NON-CHEMICAL METHODS OF DISINFECTION………………………………………………... 56 5.4 CLEANING AND DISINFECTION OF ANIMAL ENCLOSURES……………………………..……. 58 5.5 DISINFECTION OF EQUIPMENT AND TOOLS……………………………………….…………... 59 5.6 DISINFECTION AND STERILIZATION OF SUBSTRATES AND CAGE FURNITURE.…………... 59 5.7 DISINFECTION OF LIVE PLANTS………………………………………………………….……….. 61 5.8 DISINFECTION AND STERILIZATION OF WATER…………………………………….……….... 61 5.9 DISINFECTION AND BIOSECURITY IN THE FIELD……………………………………..………… 62 5.10 TABLE OF DISINFECTANTS…………………………………………………………….…….……. 66 5. 11 REFERENCES…………………………………………………………………………….….…….... 67 6. QUARANTINE…………………………………………………………………………………………..…….…... 70 6.0 INTRODUCTION………………………………………………………………………..……………. 70 6.1 GOALS AND DEVELOPMENT OF QUARANTINE PROGRAMS……………………..……...... 70 6.2 RISK ASSESSMENT IN AMPHIBIAN QUARANTINE………………………………..…………... 72 6.3 QUARANTINE FACILITY AND QUARANTINE STAFF CONSIDERATIONS………..…….….… 73 6.4 ANIMAL HUSBANDRY CONSIDERATIONS IN QUARANTINE……………….…..…… ……... 75 6.5 LENGTH OF THE QUARANTINE PERIOD……………………………………….……..….…..... 79 6.6 MEDICAL CONSIDERATIONS IN QUARANTINE…………………………………………...…… 81 6.7 LABORATORY TESTING AND DISEASE TREATMENT IN QUARANTINE…………….…..….. 84 6.8 NECROPSY……………………………………………………………………………..……..……... 85 6.9 TESTING AND TREATMENT FOR INTERNAL PARASITES………………………..…….…...…. 86 6.10 TESTING AND TREATMENT FOR AMPHIBIAN CHYTRID FUNGI……..….….….………….. 88 6.11 TESTING FOR RANAVIRUS…………………………………………………………....…….……. 93 6.12 CLINICAL PATHOLOGY (HEMATOLOGY AND CLINICAL BIOCHEMISTRY)……..…………. 96 6.13 OTHER INFECTIOUS DISEASES ENCOUNTERED IN QUARANTINE……………..….….…….97 6.14 REFERENCES…………………………………………………………………………………….….. 101 7. DIAGNOSTIC TESTING………………………………………………………………………………………….…105 7.0 INTRODUCTION…………………………………………………….……………………….….…… 106 7.1 DISEASE SURVEILLANCE………………………………………….…………………………..…… 103 7.2 EVALUATION OF LABORATORIES THAT PERFORM MOLECULAR DIAGNOSTIC TESTING………………………………………….……………..………..……. 110 7.3 DIAGNOSTIC TESTING FOR AMPHIBIAN CHYTRID FUNGI (BATRACHOCHYTRIUM DENDROBATIDIS AND BATRACHOCHYTRIUM SALAMANDRIVORANS)………….. …. 115 7.4 RANAVIRUS INFECTION…………………………………………………….…..……….……….. 132 7.5 PERKINSUS-LIKE PROTOZOAL DISEASE OF RANID FROGS…………………..…….………. 142 7.6 DISEASE REPORTING AND PARTICIPATION IN DISEASE DATABASES.………..….………. 145 7.7 TESTING AND SURVEILLANCE FOR FECAL PARASITES………………….…..…..…..…….… 146 7.8 HEMATOLOGY AND SERUM BIOCHEMISTRY……………………………….…….…………... 155 7.9 LABORATORIES THAT ACCEPT AMPHIBIAN SAMPLES………………..….…….…………... 157 7.10 REFERENCES…………………………………………………………..……………….……………159 8. TREATMENT AND CONTROL OF DISEASES……………………………………..……………….…..……... 167 8.0 INTRODUCTION………………………………………………………………………….……….…..167 8.1 TREATMENT AND CONTROL METHODS FOR AMPHIBIAN CHYTRID FUNGI…..………….168 8.2 CONTROL OF RANAVIRUS INFECTIONS…………………………………………………..….... .180 8.3 CREATING SPECIFIC-PATHOGEN-FREE AMPHIBIAN POPULATIONS……….…………….. 182 8.4 PARASITE MONITORING AND TREATMENT………………………………….…………..…… 186 8.5 ELECTROLYTE FORMULAS…………………………………………………………..………..….. 190 8.6 EUTHANASIA…………………………………………………………………………..……….….. 190 8.7 REFERENCES…………………………………………………………………………..…….……… 191 9. NECROPSY…………………………………………………………………………………………………..……..195 9.0 INTRODUCTION………………………………………………………………………….…………. 195 9.1 NECROPSY PREPARATION…………………………………………….…………..……………… 196 9.2 MATERIALS NEEDED…………………………………………………..………………………….. 196 9.3 PERFORMING THE NECROPSY……………………………………….………………………….. 197 9.4 ANATOMICAL NOTES FOR AMPHIBIAN NECROPSIES………….…………….……………… 201 9.5 SAMPLE COLLECTION DURING MORTALITY EVENTS……………….……..……………….. 204 9.6 SHIPMENT OF SAMPLES………………………………………………………..….……………… 206 9.7 REFERENCES………………………………………………………………..…….…………………. 206 APPENDIX 1: METHODS TO INDIVIDUALLY IDENTIFY THE AMPHIBIAN SPECIMEN……………….…. 208 APPENDIX 2: WASTEWATER TREATMENT......................................................................... 227 APPENDIX 3: ISOLATED AMPHIBIAN ROOMS AT OMAHA’S HENRY DOORLY ZOO…………...……. 231 APPENDIX 4 DISEASE RISK ANALYSIS AND HEALTJ SURVEILLANCE FOR THE SPECIES RECOVERY PROGRAMME ……………..……………………………………………………………………………..…………240 CHAPTER 1 INTRODUCTION The International Union for the Conservation of Nature (IUCN) has estimated that 30% of all amphibian species are threatened with extinction, and at least 484 species are Critically Endangered (http://www.iucnredlist.org). The major contributing factor of the most drastic amphibian population declines is the disease chytridiomycosis caused by amphibian chytrid fungi, specifically Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans. These fungi, disseminated by anthropogenic means, can reduce amphibian biodiversity at new locations in alarmingly short periods of time. Thus, understanding and controlling infectious diseases such as chytridiomycosis have become a major focus of both in situ and ex situ amphibian conservation efforts worldwide. To formulate a response to the crisis of global amphibian extinctions, the Species Survival Commission (SSC) of the IUCN convened