Supramolecular Studies on the Behaviour of Different Chiral

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!1A; ,  (11), 4814–4820. (228) Mohanty, A.; Dey, J. Effect of the Headgroup Structure on the Aggregation Behavior and Stability of Self-Assemblies of Sodium N-[4-(n-Dodecyloxy)benzoyl]-L-Aminoacidates in Water. *605=2:  , (3), 1033–1040. (229) El-Hachemi, Z.; Mancini, G.; Ribó, J. M.; Sorrenti, A. Role of the Hydrophobic Effect in the Transfer of Chirality from Molecules to Complex Systems: From Chiral Surfactants to Porphyrin/surfactant Aggregates. 
5
1.5
$7,  ,   (45), 15176–15184. (230) Shinitzky, M.; Haimovitz, R. Chiral Surfaces in Micelles of Enantiomeric N-Palmitoyl-and N- Stearoylserine. 
5
1.5
$7, ,  (9), 12545–12549. (231) Domínguez, A.; Fernández, A.; González, N.; Iglesias, E.; Montenegro, L. Determination of Critical Micelle Concentration of Some Surfactants by Three Techniques. 
1.5
-=, ,  (10), 1227– 1231. (232) Tahirat, N.; Luis, A.; Mindy, L. Determination of Critical Micelle Concentrations Using UV-Visible Spectroscopy. 
201
$,1
#.;  , (1), 1–6. (233) Yu, D.; Huang, F.; Xu, H. Determination of Critical Concentrations by Synchronous Fluorescence Spectrometry. 6*4
.<17-;  , (1), 47–49. (234) Tanhaei, B.; Saghatoleslami, N.; Chenar, M. P.; Ayati, A.; Hesampour, M.; Mänttäri, M. Experimental Study of CMC Evaluation in Single and Mixed Surfactant Systems, Using the UV–Vis Spectroscopic Method. 
$=:/*,<*6<;
.<.:0  ,  (3), 357–362. (235) Polavarapu, P. L.; Vijay, R. Chiroptical Spectroscopy of Surfactants. 
!1A;
1.5
  ,  (21), 5112–5118. (236) Boleda, M. D.; Briones, P.; Farrés, J.; Tyfield, L.; Pi, R. Experimental Design: A Useful Tool for PCR Optimization. 27<.,1629=.; ,  (1), 134–140. (237) Chien, A.; Edgar, D. B.; Trela, J. M. Deoxyribonucleic Acid Polymerase from the Extreme Thermophile Thermus Aquaticus. 
*,<.:274 ,   (3), 1550–1557. (238) Haugand, R. P.; Yue, S. T.; Millard, P. J.; Roth, B. L. Cyclic-Substituted Unsymmetrical Cyanine Dyes. US5436134, 1995. (239) Jadhav, V. M.; Valaske, R.; Maiti, S. Interaction between 14mer DNA Oligonucleotide and Cationic Surfactants of Various Chain Lengths. 
!1A;
1.5
  ,  (29), 8824–8831. (240) %1.
74472-*4
75*26
(1.:.
!1A;2,;
1.52;<:A
%.,167470A
*6-
27470A
..<; Evans, D. F., Wennerström, H., Eds.; VCH: New York, 1994. (241) Zhu, D.-M.; Evans, R. K. Molecular Mechanism and Thermodynamics Study of Plasmid DNA and Cationic Surfactants Interactions. *605=2:  , (8), 3735–3743. (242) Diamant, H.; Andelman, D. Self-Assembly in Mixtures of Polymers and Small Associating Molecules. *,:7574.,=4.;  , (21), 8050–8061. (243) Kypr, J.; Kejnovská, I.; Ren iuk, D.; Vorlí ková, M. Circular Dichroism and Conformational Polymorphism of DNA. =,4.2,
,2-;
#.;  ,  (6), 1713–1725. (244) Grueso, E.; Kuliszewska, E.; Roldan, E.; Perez-Tejeda, P.; Prado-Gotor, R.; Brecker, L. DNA Conformational Changes Induced by Cationic Gemini Surfactants: The Key to Switching DNA Compact Structures into Elongated Forms. #$
->  , (37), 29433–29446. (245) Khouri, S. J.; Buss, V. Circular Dichroism and Derivative Spectra Study of the Excitonic Aggregation of Pinacyanol by Aerosol-OT. 8.6

!1A;
1.5  , , 34–40. (246) Chang, Y.-M.; Chen, C. K.-M.; Hou, M.-H. Conformational Changes in DNA upon Ligand Binding Monitored by Circular Dichroism. 6<

74
$,2  ,  (3), 3394–3413. (247) 1*8<.:
  "=*6<2<*<2>.
 ;.
77<8:26<
%2<:*<276

.<17- /7:
$<=-A260
!:7<.26 
6<.:*,<276;; Brenowitz, M., Senear, D. F., Shea, M. A., Ackers, G. K., Eds.; Methods in Enzymology; Elsevier, 1986; Vol. 130. (248) Watson, R. J.; Schildkraut, I.; Qiang, B.-Q.; Martin, S. M.; Visentin, L. P. NdeI: A Restriction Endonuclease from Neisseria Denitrificans Which Cleaves DNA at 5 -CATATG-3 Sequences. $
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1.5  ,  , 6004–6011. (253) Engel, R. H.; Riggit, S. J. Intestinal Absorption of Heparin Facilitated by Sulfated or Sulfonated Surfactants. 
!1*:5
$,2 ,  (6), 706–710. (254) Song, W. W.; Li, N. B.; Luo, H. Q. Gemini Surfactant Applied to the Heparin Assay at the Nanogram Level by Resonance Rayleigh Scattering Method. 6*4
27,1.5  , (1), 1–6. (255) Sun, W.; Han, J.; Li, Q.; Jiao, K. Spectrophotometric and Voltammetric Studies on the Interaction of Heparin with Crystal Violet and Its Analytical Application. $7=<1
/:2,*6

1.5  , , 42–46. (256) Yongliang, C.; Malek, M.; Gulilat, G. Transfection Reagents. US8785200(B2), 2012. (257) Zhou, T.; Llizo, A.; Li, P.; Wang, C.; Guo, Y.; Ao, M.; Bai, L. High Transfection Efficiency of Homogeneous DNA Nanoparticles Induced by Imidazolium Gemini Surfactant as Nonviral Vector.  !1A;
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$,2  ,  (12), 3103–3111. (260) Cardoso, A. M.; Morais, C. M.; Cruz, A. R.; Silva, S. G.; Luísa, M.; Marques, E. F.; Pedroso, M. C.; Lima, D.; Jurado, A. S. New Serine-Derived Gemini Surfactants as Gene Delivery Systems. =:

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  ,  (32), 8172–8180. (286) Castiglioni, E.; Biscarini, P.; Abbate, S. Experimental Aspects of Solid State Circular Dichroism. 12:*42

296 12. Annex


BB9L

297 12. Annex

298 12. Annex


BB9L


FMC-&
=A5;9G
C:
D! 89D9B89BH
GIF:57H5BHG

25000X 25000X

50000X 50000X

20000X 15000X

=;IF9 . CryoTEM images of   at three different pH’s: 2 (top), natural (middle) and 12 (bottom).

299 12. Annex

20000X 20000X

5000X 12000X

15000X 12000X

=;IF9 . CryoTEM images of -  at three different pH’s: 2 (top), natural (middle) and 12 (bottom).

300 12. Annex

15000X 5000X

3000X 15000X

25000X 20000X

=;IF9 . CryoTEM images of   at three different pH’s: 2 (top), natural (middle) and 12 (bottom).

301 12. Annex

12000X 12000X

15000X 3000X

30000X 5000X

=;IF9 . CryoTEM images of -  at three different pH’s: 2 (top), natural (middle) and 12 (bottom).

302 12. Annex

 
CFF9@5H=CB
7IFJ9G
C:
%,

a) b)

1.0 1.0

0.8 0.8 C12 1 T12 1 C12 2 T12 2 0.6 C12 3 0.6 T12 3

0.4 0.4 Correlation Coefficient 0.2 Correlation Coefficient 0.2

0.0 0.0

10-1 100 101 102 103 104 105 106 107 108 10-1 100 101 102 103 104 105 106 107 108 c)Time (μs) d) Time (μs)

1.0 1.0 T16 1 C16 1 T16 2 0.8 C16 2 0.8 T16 3 C16 3

0.6 0.6

0.4 0.4

Correlation Coefficient 0.2 Correlation Coefficient 0.2

0.0 0.0

10-1 100 101 102 103 104 105 106 107 108 10-1 100 101 102 103 104 105 106 107 108 Time (μs) Time (μs)

=;IF9 . Correlation function of the DLS measurements of surfactant a)  , b) - , c)   and d) - .

303 12. Annex

  A=GG=CB
,D97HF5
C:
' 9@,H5FO 7CAD@9L9G
5H
8=::9F9BH GIF:57H5BH
7CB79BHF5H=CBG

1.2 1.2

1.0 C12 1.0 T12 0.8 0.8

0.6 0.6

0.4

Normalized Intensity 0.4 Normalized Intensity

0.2 0.2

0.0 0.0 540 560 580 600 620 640 660 680 700 540 560 580 600 620 640 660 680 700 1.2 Wavelength (nm) 1.2 Wavelength (nm)

1.0 1.0 C16 T16

0.8 0.8

0.6 0.6

0.4 0.4 Normalized Intensity Normalized Intensity

0.2 0.2

0.0 0.0 540 560 580 600 620 640 660 680 700 540 560 580 600 620 640 660 680 700 Wavelength (nm) Wavelength (nm)

1.4 1.4

1.2 1.2 CTAB DTAB 1.0 1.0

0.8 0.8

0.6 0.6 Normalized Intensity

Normalized Intensity 0.4 0.4

0.2 0.2

0.0 0.0 540 560 580 600 620 640 660 680 700 540 560 580 600 620 640 660 680 700 Wavelength (nm) Wavelength (nm)

=;IF9 . Emission Spectra of DNA-GelStar complexes at different surfactant concentrations.

304 12. Annex

 
CAD5F=GCB
69HK99B
H<9
F9GI@HG
C6H5=B98
:FCA

5B8 &,

To compare the obtained results, the superposition of the DEA and the EMSA results were represented. These two techniques give us different information about the formed complexes, but the behaviour of these surfactants with DNA can be compared.

Superposed graphics are shown in Figure 148. Blue line represents the Zhp-3 and purple line show the CMC of the surfactant.

1.2 1.0 C12 1.0 T12 0.8 DEA DEA EMSA 0.8 EMSA 0.6 0.6

0.4 0.4 Normalized data Normalized data 0.2 0.2

0.0 0.0

0.1 1 10 100 1000 10000 0.1 1 10 100 1000 10000 Concentration (μM) Concentration (μM) 1.0 1.0 C16 T16 0.8 DEA 0.8 DEA EMSA EMSA

0.6 0.6

0.4 0.4 Normalized Intensity 0.2 Normalized Intensity 0.2

0.0 0.0

0.1 1 10 100 1000 0.1 1 10 100 1000 Concentration (μM) Concentration (μM)

1.0 1.4

CTAB 1.2 DTAB 0.8 DEA 1.0 EMSA DEA EMSA 0.6 0.8

0.6 0.4 0.4 Normalized Intensity 0.2 Normalized Intensity 0.2

0.0 0.0 -0.2 0.1 1 10 100 1000 1 10 100 1000 10000 Concentration (μM) Concentration (μM) =;IF9 . Superposed results of DEA and EMSA experiments. Blue line represents the

3 Z

Results of both techniques are in very good agreement with each other and they verify that all the obtained results are reproducible and correct.

As it is shown, some information about the mechanism of the formation of the complexes can be find out. Difference between the 12-carbon and 16-carbon chain length can be observed. On the one hand, in both   and - , the concentration of the critical intensity (from DEA) corresponds to the starting point of the decrease of the free DNA concentration (from EMSA), while in   and - , the concentration of the critical intensity (from DEA) corresponds to the final point of the decrease of the free DNA concentration (from EMSA). It clearly means that the mechanism of the complex formation is different depending on the chain length and it seems to be independent on the stereochemistry.

Once the concentration of surfactant is above the CAC, surfactant aggregates start to form a complex with free DNA. In the case of   and - , the binding avoids GelStar® to bind DNA, reducing the intensity in DEA but they do not decrease the negative charges of the DNA. Once the CCI is reached, concentration of free DNA starts to decrease. After the Zhp-2 (in DEA), they show a broad peak where the intensity is increased and then it is decreased again. Comparing the results of DEA and EMSA, it is observed that the maximum of this broad peak corresponds to the Zhp-3 (blue line). After this point, the formation of the positively charged complex or the precipitation starts.

On the other hand,   and -  not only avoid GelStar® to bind DNA but also reduce the negative charges of the DNA until the Zhp-2 is reached. Then, the non-charged complex is obtained (Zhp-3, blue line) and the formation of the positively charged complex or the precipitation starts.

In all cases, CMC is above the concentration at which the positively charged complex starts to be formed. The formation of micelles (or other surfactant aggregates) in solution favours that these new aggregates bind DNA forming the cationic complex.

306 12. Annex

  '5G9
CCH
)F=BH=B;
GG5M

NruI:

=;IF9 . Resulting images from the electrophoresis of the NruI DNAse Foot Printing Assays. Concentrations are shown in μM of surfactant/ppm of DNA.

307 12. Annex

Standard DNAse:

=;IF9  . Resulting images from the electrophoresis of the DNAse Foot Printing Assays. Concentrations are shown in μM of surfactant/ppm of DNA.

308 12. Annex

  -F5BG:97H=CB
GHI8=9G

=;IF9  . Images of the cells and images of the fluorescence microscope of the transfection studies of compounds DTAB, -  and  .

309 12. Annex

=;IF9 . Images of the cells and mages of the fluorescence microscope of the transfection studies of compounds CTAB, -  and  .

310 12. Annex

  )FC;F5A
d5@7I@Ge

Calculs is a program created using Bash code using the terminal to control the status of the computational calculations and to show which calculations are finished. Additionally, it shows all the useful information of each calculation.

Example of the output of the program:

Picard:/users/bernat>calculs

*******************************************************************************************************************************************

Hola bernat! Avui es dia 10/03/16, son les 09:48:47 i aquests son els teus calculs:

*******************************************************************************************************************************************

BORG2: max 12 jobs, 96 procs

Job ID Username Queue Jobname NDS Nodes S Time Main Borg Starting time ------156059.kirk.uab bernat borg2 trans16h-monomer-optbp.gjf 1 12 R 07:13 borg2-21 Mar 10 02:35:06 156074.kirk.uab bernat borg2 trans16-monomer-spbg.xyz 1 12 R 00:47 borg2-29 Mar 10 09:00:31

# Total borg2: 2 (2 + 0) # Estas fent servir 24 procs

********************************************************************************************************************************************

ACABATS: trans16-monomer-optbp.xyz (borg1)

********************************************************************************************************************************************

COMENTARIS:

******************************************* πππππππππππππππππππππππππππ *******************************************

Code of the program:

#!/bin/bash

# AQUEST ES EL FITXER FINAL DEL PROGRAMA. # SI VOLS UTILITZAR-LO, PRIMER HAS DE CREAR UNA CARPETA QUE ES DIGUI estatcalculs DINS DE LA TEVA CARPETA bin. # CANVIA EL TEU NOM D’USUARI. ALLA ON POSA bernat, POSA-HI EL TEU NOM. POTS SUBSTITUIR EL NOM DE TOT EL FITXER. # ASSEGURA’T QUE LES CUES ON TU CORRES ESTAN ACTIVADES --> NO HAN DE TENIR EL SIMBOL # AL DAVANT. # JA POTS EXECUTAR EL PROGRAMA. LA PRIMERA VEGADA QUE L’EXECUTIS ET SORTIRA ALGUN ERROR PERQUE S’HAN D’AUTOGENERAR UNS FITXERS. # A PARTIR D’ARA JA NOMES CAL QUE FACIS SERVIR EL PROGRAMA. SI EL TENS DINS DE LA CARPETA bin, NOMES ESCRIVINT calculs PODRAS EXECUTAR-LO. # PER ELIMINAR ELS CALCULS ACABATS, HAS D’ANAR A LA CARPETA estatcalculs I OBRIR EL FITXER acabatsnomdelacua (p.ex. acabatsborg1). # ELIMINA EL NOM DEL CALCUL QUE HAGI ACABAT I SURT DEL FITXER FENT :wq, DEIXANT EL FITXER EN BLANC. # POTS EDITAR EL .bashrc PER TAL DE POSAR UN ALIAS PER OBRIR AQUESTS FITXERS. PER EXEMPLE, endb1 POT SERVIR PER VEURE ELS FITXERS QUE HAN ACABAT A BORG1. # ENDAVANT I GAUDIR DEL PROGRAMA! # NO CAL DIR QUE QUALSEVOL ERROR QUE TROBIS O QUALSEVOL MILLORA, ESTAREM ENCANTATS DE QUE ENS HO DIGUIS. #Darrera actualitzacio: 06/02/2014 16:36 clear tinccalculs=$(qstat -u bernat |grepbernat -c) if [ "$tinccalculs"==0 ];then echo "" echo -e "\e[0;31m ******************************************************************************************************************* \e[0m" echo "" echo "" echo "NO TENS CAP CALCUL CORRENT!" echo "" echo "" echo -e " \e[0;36mACABATS:\e[0m" grep . /users/bernat/bin/estatcalculs/acabats_borg1 | awk ’{print " "$0" (borg1)"}’ grep . /users/bernat/bin/estatcalculs/acabats_borg2 | awk ’{print " "$0" (borg2)"}’ grep . /users/bernat/bin/estatcalculs/acabats_borg3 | awk ’{print " "$0" (borg3)"}’

311 12. Annex

echo "" echo -e "\e[0;31m ******************************************************************************************************************* \e[0m" echo "" exit fi touch /users/bernat/bin/estatcalculs/noms_antic1 touch /users/bernat/bin/estatcalculs/noms_antic2 touch /users/bernat/bin/estatcalculs/noms_antic3 touch /users/bernat/bin/estatcalculs/date_antic touch /users/bernat/bin/estatcalculs/num_antic username=$(whoami) hora=$(date | awk ’{print $4}’) dia=$(date +"%d/%m/%y") echo "" echo -e "\e[0;31m ******************************************************************************************************************* \e[0m" echo "" echo -e "\e[0;36m Hola ${username}! Avui es dia $dia, son les $hora i aquests son els teus calculs: \e[0m" echo "" gruppropi=$(id -gn) grup=$(infocues $gruppropi |grep"Estat"|awk ’{print $6}’) for i in $grup;do cua=$(qstat -u $username $i) if test -n "$cua";then total=$(qstat -u ${username} $i |grep$username -c) #a = Job Id, Username and Queue a=$(qstat $i -u ${username} | awk ’NR>5 {print " "$1"s" " " $2 " " $3 " " }’) #b = Jobname b=$(qstat -f $(qstat -u ${username} $i | awk ’NR>5 {print $1"es"}’) | grep -e "Job_Name"|awk ’{print $3}’) #c = NDS and Nodes c=$(qstat $i -n1u ${username} | awk ’NR>5 {print " "$6 " " $7}’) #d = Status and Time d=$(qstat $i -n1u ${username} | awk ’NR>5 {print $10 " " $11}’) #e = starting date e=$(qstat -f $(qstat -u ${username} $i | awk ’NR>5 {print $1"es"}’) | grep -e "start_time"|awk ’{print $4 " " $5 " " $6}’)

#nombre de calculs run_borg=$(qstat -u $username $i |grep " R " -c) queue_borg=$(qstat -u $username $i |grep " Q " -c) maxprocs=$(infocues $i | awk ’NR>9 && NR<11 {print $3}’)

# comença el programa! echo " Job ID Username Queue">>output echo " ------">>output if test -n "${total}";then echo "${a}">>output echo "Jobname">>output echo "------">>output echo "${b}">>output echo "NDS Nodes">>output echo "------">>output echo "${c}">>output echo "S Time">>output echo "-----">>output echo "${d}">>output echo "Main Borg">>output echo "------">>output long=$(qstat $i -n1u ${username} | awk ’NR>5 {print $12 }’) for j in ${long};do short=${j:0:8} echo $short >> output done echo "Starting time">>output echo "------">>output echo "${e}">>output fi

#fila on comença cada bloc inb1=$((0 + 1)) inb2=$((0 + $total + 3)) inb3=$((0 + 2 * $total + 5)) inb3b=$((0 + 3 * $total + 7)) inb4=$((0 + 4 * $total + 9)) inb5=$((0 + 5 * $total + 11))

#fila on acaba cada bloc fib1=$((0 + $total + 2 )) fib2=$((0 + 2 * $total + 4)) fib3=$((0 + 3 * $total + 6)) fib3b=$((0 + 4 * $total + 8)) fib4=$((0 + 5 * $total + 10)) fib5=$((0 + 5 * $total + $run_borg + 12))

#el que conte cada bloc 312 12. Annex

(sed -n ${inb1},${fib1}p output)>file1 (sed -n ${inb2},${fib2}p output)>file2 (sed -n ${inb3},${fib3}p output)>file3 (sed -n ${inb3b},${fib3b}p output)>file4 (sed -n ${inb4},${fib4}p output)>file5 (sed -n ${inb5},${fib5}p output)>file6 echo -e "\e[0;31m ******************************************************************************************************************* \e[0m" echo "" if [ "$i"=="borg1" ];then echo -e "\e[0;32m BORG1\e[0m: max 10 jobs, 32 procs" else if [ "$i"=="borg2" ];then echo -e "\e[0;32m BORG2\e[0m: max 12 jobs, 96 procs" else if [ "$i"=="borg3" ];then echo -e "\e[0;32m BORG3\e[0m: " fi fi fi

echo "" paste file1 file2 file3 file4 file5 file6 | column -s $’\t’-t echo "" echo " # Total $i: $total ($run_borg + $queue_borg)" suma=$(qstat -u $username $i |grep’R ’|awk ’{print $7}’) n=0 for k in $suma;do n=$(($k + $n)) done echo " # Estas fent servir $n procs" echo "" #elimina els fitxers temporals creats rm file{1..6} rm output fi done echo -e "\e[0;31m ******************************************************************************************************************* \e[0m" totalborg1=$(qstat -u bernat borg1) totalborg2=$(qstat -u bernat borg2) totalborg3=$(qstat -u bernat borg3) total=$(qstat -u bernat |grepbernat -c) antic1=$(cat /users/bernat/bin/estatcalculs/noms_antic1) antic2=$(cat /users/bernat/bin/estatcalculs/noms_antic2) antic3=$(cat /users/bernat/bin/estatcalculs/noms_antic3) dateantic=$(cat /users/bernat/bin/estatcalculs/date_antic | awk ’{print $2 " " $3 " " $4}’) if test -n "$totalborg1";then noms1=$(qstat -f $(qstat -u ${username} borg1 | awk ’NR>5 {print $1"es"}’) | grep -e "Job_Name"|awk ’{print $3}’) echo "$noms1">>actual1 for i in $antic1;do comproba1=$(grep "$i" actual1 |grep"$i" -c) if [ "$comproba1"==0 ];then echo $i >> /users/bernat/bin/estatcalculs/acabats_borg1 fi done rm actual1 else for i in $antic1;do echo $i >> /users/bernat/bin/estatcalculs/acabats_borg1 done fi if test -n "$totalborg2";then noms2=$(qstat -f $(qstat -u ${username} borg2 | awk ’NR>5 {print $1"es"}’) | grep -e "Job_Name"|awk ’{print $3}’) echo "$noms2">>kk2 for i in $antic2;do comproba=$(grep "$i" kk2 |grep"$i" -c) if [ "$comproba"==0 ];then echo $i >> /users/bernat/bin/estatcalculs/acabats_borg2 fi done rm kk2 else for i in $antic2;do comproba2=$(grep "$i" /users/bernat/bin/estatcalculs/acabats_borg2 |grep"$i" -c) if [ "$comproba2"==0 ];then echo $i >> /users/bernat/bin/estatcalculs/acabats_borg2 fi done fi if test -n "$totalborg3";then noms3=$(qstat -f $(qstat -u ${username} borg3 | awk ’NR>5 {print $1"es"}’) | grep -e "Job_Name"|awk ’{print $3}’) echo "$noms3">>kk3 for i in $antic3;do comproba=$(grep "$i" kk3 |grep"$i" -c) 313 12. Annex

if [ "$comproba"==0 ];then echo $i >> /users/bernat/bin/estatcalculs/acabats_borg3 fi done rm kk3 else for i in $antic3;do comproba3=$(grep "$i" /users/bernat/bin/estatcalculs/acabats_borg3 |grep"$i" -c) if [ "$comproba3"==0 ];then echo $i >> /users/bernat/bin/estatcalculs/acabats_borg3 fi done fi if test -n "$totalborg1";then antic1_bis=$(qstat -f $(qstat -u ${username} borg1 | awk ’NR>5 {print $1"es"}’) | grep -e "Job_Name"|awk ’{print $3}’) echo "$antic1_bis">/users/bernat/bin/estatcalculs/noms_antic1 else echo "" > /users/bernat/bin/estatcalculs/noms_antic1 fi if test -n "$totalborg2";then antic2_bis=$(qstat -f $(qstat -u ${username} borg2 | awk ’NR>5 {print $1"es"}’) | grep -e "Job_Name"|awk ’{print $3}’) echo "$antic2_bis">/users/bernat/bin/estatcalculs/noms_antic2 else echo "" > /users/bernat/bin/estatcalculs/noms_antic2 fi if test -n "$totalborg3";then antic3_bis=$(qstat -f $(qstat -u ${username} borg3 | awk ’NR>5 {print $1"es"}’) | grep -e "Job_Name"|awk ’{print $3}’) echo "$antic3_bis">/users/bernat/bin/estatcalculs/noms_antic3 else echo "" > /users/bernat/bin/estatcalculs/noms_antic3 fi date > /users/bernat/bin/estatcalculs/date_antic numcalcantic=$(cat /users/bernat/bin/estatcalculs/num_antic) numcalcactual=$(qstat -u bernat |grepbernat -c) resta=$(($numcalcantic - $numcalcactual)) qstat -u bernat | grep bernat -c > /users/bernat/bin/estatcalculs/num_antic echo "" echo -e " \e[0;36mACABATS:\e[0m" touch /users/bernat/bin/estatcalculs/acabats_borg1 touch /users/bernat/bin/estatcalculs/acabats_borg2 touch /users/bernat/bin/estatcalculs/acabats_borg3 grep . /users/bernat/bin/estatcalculs/acabats_borg1 | awk ’{print " "$0" (borg1)"}’ grep . /users/bernat/bin/estatcalculs/acabats_borg2 | awk ’{print " "$0" (borg2)"}’ grep . /users/bernat/bin/estatcalculs/acabats_borg3 | awk ’{print " "$0" (borg3)"}’ echo "" echo -e "\e[0;31m ******************************************************************************************************************* \e[0m" echo "" echo -e " \e[0;36mCOMENTARIS:\e[0m" grep . /users/bernat/bin/estatcalculs/comentaris_calculs | awk ’{print " "$0}’ echo "" echo -e "\e[0;31m ******************************************* \e[1;33m?~@?~@?~@\e[m\e[1;31m?~@?~@?~@\e[m\e[1;33m?~@?~@?~@\e[m\e[1;31m?~@?~@?~@\e[m\e[1;33m?~@?~@?~@\e[m\ e[1;31m?~@?~@?~@\e[m\e[1;33m?~@?~@?~@\e[m\e[1;31m?~@?~@?~@\e[m\e[1;33m?~@?~@?~@\e[m\e[0;31m ******************************************* \e[0m" echo "" # AUTORS: Pi-Boleda, B.; Navarro, J. # Amb la col·laboració de: Canales, I. End of the program.

314 12. Annex

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,MADCG=IA Berlin, June 2015

315 12. Annex

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,MADCG=IA Berlin, June 2015

316 12. Annex

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C7HCF5@G Bellaterra, June 2016

It was awarded with the First Price.

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