1 Revista Agrotecnologia, Ipameri, V.11, N.2, P.1-13, 2020 WOLFAPPLE
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WOLFAPPLE (SOLANUM LYCOCARPUM ST. HILL) STARCH: CHEMICAL AND PHYSICAL PROPERTIES FRUTA-DO-LOBO (SOLANUM LYCOCARPUM ST. HILL): PROPRIEDADES QUÍMICAS E FÍSICAS DO AMIDO. Adriana dos Santos Fernandes1, Diego Palmiro Ramirez Ascheri1, Marcos Paulo Batista2, Jocielle Conceição Oliveira Cardoso3, Jacqueline Nascimento Gomes3, Yago Silva de Sousa1. Abstract: In this study, starch from S. informações sobre sua caracterização. Para lycocarpum fruit (wolfapple) was extracted to isto, realizou-se extração do amido e o cálculo determine physical and chemical properties do seu rendimento. Determinou-se também sua and provide more information and further composição química (cinzas, extrato etéreo, evidence on its characteristics. Starch yield fibras, proteínas e teor de amilose); forma e was calculated. The chemical composition of tamanho dos grânulos; análise de viscosidade the wolfapple starch was determined for ash, (RVA); análise calorimétrica (DSC) e análise ether extract, fiber, protein and amylose cristalográfica. A extração do amido da fruta content. The shape and size of the granules de S. lycocarpum teve um rendimento de were assessed, as well as rapid visco analysis 27,9% e este se caracterizou por apresentar (RVA); differential scanning calorimetry 0,19% ±0,01 de cinzas, 0,07% ± 0,00 de (DSC); and crystallographic analysis. The extrato etéreo, 0,32% ± 0,01de proteínas, yield of the wolfapple starch was 27.9% and 0,03% ± 0,01 de fibras e 29,16% ± 0,09 de the chemical composition was characterized amilose. A análise de imagem do amido by the presence of 0.19% ± 0.01 ash, 0.07% ± mostrou grânulos com formato cônico e 0.00 ether extract, 0.32 % ± 0.01% proteins, diâmetros médios e respectivos desvios padrão 0.03% ± 0.01 fibers, and 29.16% ± 0.09 de 16,59±3,68 e 16,52±3,36 μm, para diâmetro amylose. Starch image analysis presented maior e menor, respectivamente. As análises conical shaped granules with average da viscosidade de pasta; calorimétrica e diameters and respective standard deviations cristalográfica revelou que o amido da fruta de of 16.59 ± 3.68 and 16.52 ± 3.36 μm, for the S. lycocarpum mostrou perfil com largest and smallest diameter, respectively. características desejáveis, como boa Paste viscosity, calorimetric, and estabilidade a alta temperatura, o que o elege crystallographic analyses showed that the como um bom ingrediente a ser usado em starch from wolfapple has desirable diversos setores alimentícios e industriais. characteristics such as stability at high temperature, making it a promising material in PALAVRAS-CHAVE: Amilose, gelatinização, various food and industrial sectors. grânulos de amido. KEYWORDS: Amylose, gelatinization, 1Universidade Estadual de Goiás, Campus CET starch granule. - Henrique Santillo. [email protected]; 2Universidade Federal de Goiás, Campus Resumo: O objetivo deste trabalho foi extrair Samambaia. e determinar as propriedades físicas e químicas 3Instituto Federal de Goiás. do amido da fruta de S. lycocarpum (fruta-do- lobo) visando obter e disponibilizar mais 1 Revista Agrotecnologia, Ipameri, v.11, n.2, p.1-13, 2020 INTRODUCTION consumed by humans (SPIER, 2010). Starch is a plant-produced polymer Starch can be extracted from cereal grains, stored in small and semicrystalline granules vegetables and tubers, and has a variety of composed by two main components: food and non-food applications amylose and amylopectin. Amylose (SCHIRMER et al., 2013; FERNANDES et predominantly consists of linear chains, al., 2019). typically consisting of α (1-4) D-glucose The starch industry extracts starch units, while amylopectin has large and from various sources and processes it into a branched chains formed by α (1-4) D- wide variety of products, such as native glucose units in the rectilinear portions and starches, glucose, maltose and fructose α bonds ( 1-6) in the branches (BENINCA syrups, dextrose and maltodextrins et al., 2013). It is the main source of reserve (CEREDA, 2002). Figure 1 shows some carbohydrate in plants and an important applications of starch, both in natural and source of energy for many organisms, modified forms. providing between 70 to 80% of the calories Figure 1. Main applications of starch in Brazil. Source: Adapted from Beninca et al, 2013. Among other usages in the food used due to its viscosity, gelling power, industries, starch can facilitate food adhesion, tendency to retrograde, among processing and is used as thickener in soups, other properties affected by the broths, and meat sauces. It provides amylose/amylopectin ratio as well as the suspended solids and texture, gelling agents shape and size of granules (CIACCO; in cured processed meat, stabilizers in salad CRUZ, 1987). dressings, and increase the shelf life of In the textile industry, starch is used products (CEREDA, 2002). Starch is also for gumming threads to facilitate weaving 2 Revista Agrotecnologia, Ipameri, v.11, n.2, p.1-13, 2020 and in papermaking to produce papers of The most prominent starchy species different strengths and high print quality in the world market are corn, potato, and (BENINCA, et al., 2013). In glue and cassava. However, recent studies have adhesive manufacturing, in general, it is searched for new sources of starch with used for providing more viscous products, potential to be used in various sectors for its easy preparation and for combining (DHANAPAL et al., 2012; KASIRAJAN; with various resins and synthetic emulsions NGOUAJIO, 2012). Industrial applications (CIACCO; CRUZ, 1987). It can be used in consider availability and physicochemical the composition of biodegradable characteristics of the starch, which may packaging (FERNANDES et al., 2019). In vary according to its origin. Thus, every culture media, starch can be useful as an natural or native starch is considered unique inductor of the production of enzymes and (MATSUGUMA et al., 2009). microbial biomass. In addition, it can be The Cerrado region in Brazil has a hydrolyzed and the sugar produced from variety of plants with very specific hydrolysis can be fermented to produce characteristics due to their adaptations to ethanol used for food, beverage, the climate and soil. These plants are still pharmacological and laboratory purposes little explored and could be used as a source (AJIBOLA et al., 2012). The glucose of starch. Solanum lycocarpum St. Hill , obtained from starch is also widely used in commonly known as wolfapple, belongs to the manufacture of organic acids such as the family Solanaceae, the most common citric acid, glutamic acid, lactic acid, and genus found in the Brazilian Cerrado amino acids such as lysine, or (Figure 2). It can produce from 40 to 100 commercialized as crystalline glucose. fruits, of masses varying between 400 to Therefore, it is very important to have 900g, with good potential for starch different starch sources available for this extraction (CAMPOS, 1994; very wide market (AQUINO et al., 2015). FERNANDES et al.; 2019). 3 Revista Agrotecnologia, Ipameri, v.11, n.2, p.1-13, 2020 Figure 2. S. lycocarpum a) plant; b) flower; c) and d) fruit cross section. Source: Adapted from Campos (1994). In the present study, starch from S. properties and provide more information lycocarpum fruit (wolfapple) was extracted and further evidence on its characteristics. to determine physical and chemical MATERIAL AND METHODS washed under running water for starch S. lycocarpum fruits were collected in separation and decantation in plastic the rural area of the municipality of containers. Following decantation, the Anápolis-GO. Pulp samples were collected material was washed with absolute alcohol in paper bags and stored in a refrigerator for to remove resins and vacuum filtered. After starch extraction. purification, the starch was vacuum dried at To obtain the starch, the pulp of the 40°C to constant weight. The dehydrated peeled fruits was cut into small pieces with starch was kept in a desiccator containing a stainless steel knife and stored in plastic silica gel for 12h and reduced to powder buckets containing a 5 g.L-1 sodium using mortar and pestle. Finally, the metabisulphite solution to prevent material was sieved to 48 mesh to obtain the browning. dry sample for analysis. The pulp was milled in a The yield was calculated as the ratio “CROTON” MA580 knife mill between the weight of the peeled pulp and (MARCONI, Piracicaba, Brazil) and sieved the starch produced. The material was to 10 mesh (1700 µm). The pulp was again characterized considering ash, fiber, ether sieved (150 to 400 mesh), and repeatedly extract, and protein content (AOAC, 2000). 4 Revista Agrotecnologia, Ipameri, v.11, n.2, p.1-13, 2020 Amylose content was determined according Calorimetric analysis was performed to the methodology proposed by Williams on a DSC Q200 differential scanning et al. (1970). calorimeter (TA Instruments, New Castle, Image analysis was used to evaluate USA), using indium calibration. To the shape and size of the isolated starch determine the gelatinization temperature, granules. The samples were collected with approximately 5.0 mg of the sample (at platinum wire on a glass slide, mixed with a known moisture) was placed into an airtight drop of distilled water, and covered with a aluminum container. The scanning profile coverslip. The slides were examined with a used was balanced at 5ºC, heating at 110 LEICA DME optical microscope (Wetzlar, ºC, heating rate of 10ºC.min-1, and flow of Germany), and the selected images were 50 mL nitrogen per minute. Gelatinization analyzed with the software LAS EZ. enthalpy