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WO 2017/105987 Al 22 June 2017 (22.06.2017) W P O P C T

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WO 2017/105987 Al 22 June 2017 (22.06.2017) W P O P C T (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/105987 Al 22 June 2017 (22.06.2017) W P O P C T (51) International Patent Classification: Ave, Po Box 552, Johnston, Iowa 5013 1 (US). ORAL, C07K 14/21 (2006.01) C12R 1/38 (2006.01) Jarred Kenneth; 7250 Nw 62nd Ave, Po Box 552, John C12N 15/82 (2006.01) A01N 63/02 (2006.01) ston, Iowa 5013 1 (US). PEREZ-ORTEGA, Claudia; 7250 Nw 62nd Ave, Po Box 552, Johnston, Iowa 5013 1 (21) International Application Number: (US). ROSEN, Barbara Ann Marie; 7250 Nw 62nd Ave, PCT/US2016/06553 1 Po Box 552, Johnston, Iowa 501 3 1 (US). SCHELLEN- (22) International Filing Date: BERGER, Ute; 914 Moreno Avenue, Palo Alto, Califor 8 December 2016 (08.12.2016) nia 94303 (US). WEI, Jun-Zhi; 7250 Nw 62nd Ave, Po Box 552, Johnston, Iowa 5013 1 (US). XIE, Weiping; (25) Filing Language: English 7250 Nw 62nd Ave, Po Box 552, Johnston, Iowa 5013 1 (26) Publication Language: English (US). ZHONG, Xiaohong; 7250 Nw 62nd Ave, Po Box 552, Johnston, Iowa 5013 1 (US). ZHU, Genhai; 7250 Nw (30) Priority Data: 62nd Ave, Po Box 552, Johnston, Iowa 5013 1 (US). 62/269,482 18 December 201 5 (18. 12.2015) US (74) Agent: BAUER, S., Christopher; PIONEER HI-BRED (71) Applicants: PIONEER HI-BRED INTERNATIONAL, INTERNATIONAL, INC., 7100 N.W. 62nd Avenue, P.O. INC. [US/US]; 7100 N.W. 62nd Avenue, P.O. Box 1014, Box 1014, Johnston, Iowa 5013 1-1014 (US). Johnston, Iowa 5013 1-1014 (US). E I DU PONT DE NEMOURS AND COMPANY [US/US]; Chestnut Run (81) Designated States (unless otherwise indicated, for every Plaza, 974 Centre Road, P.O. Box 291 5, Wilmington, kind of national protection available): AE, AG, AL, AM, Delaware 19805 (US). AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (72) Inventors: LIU, Lu; 7250 Nw 62nd Ave, Po Box 552, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, Johnston, Iowa 5013 1 (US). LUM, Amy; 7250 Nw 62nd HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, [Continued on nextpage] (54) Title: INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE (57) Abstract: Compositions and methods for controlling pests are Fig. 1A provided. The methods involve transforming organisms with a nucle ic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microor ganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for sub - sequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homolog ous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemi- pteran and nematode pest populations and for producing composi tions with insecticidal activity. ™ < 00 o o o w o 2017/105987 AI III III II II III III ill II 11llll III I lllll II I II KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). VN, ZA, ZM, ZW. Published: (84) Designated States (unless otherwise indicated, for every — with international search report (Art. 21(3)) kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, — with sequence listing part of description (Rule 5.2(a)) TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE CROSS-REFERENCE TO RELATED APPLICATIONS This application claims priority to United States Provisional Application No. 61/269482, filed December 18, 201 5, which is hereby incorporated herein in its entirety by reference. REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY The official copy of the sequence listing is submitted electronically via EFS-Web as an ASCII formatted sequence listing with a file named "6048WOPCT_Sequence_Listing" created on September 19, 2016, and having a size of 172 kilobytes and is filed concurrently with the specification. The sequence listing contained in this ASCII formatted document is part of the specification and is herein incorporated by reference in its entirety. FIELD This disclosure relates to the field of molecular biology. Provided are novel genes that encode pesticidal proteins. These pesticidal proteins and the nucleic acid sequences that encode them are useful in preparing pesticidal formulations and in the production of transgenic pest-resistant plants. BACKGROUND Biological control of insect pests of agricultural significance using a microbial agent, such as fungi, bacteria or another species of insect affords an environmentally friendly and commercially attractive alternative to synthetic chemical pesticides. Generally speaking, the use of biopesticides presents a lower risk of pollution and environmental hazards and biopesticides provide greater target specificity than is characteristic of traditional broad- spectrum chemical insecticides. In addition, biopesticides often cost less to produce and thus improve economic yield for a wide variety of crops. Certain species of microorganisms of the genus Bacillus are known to possess pesticidal activity against a range of insect pests including Lepidoptera, Diptera, Coleoptera, Hemiptera and others. Bacillus thuringiensis {Bt) and Bacillus popilliae are among the most successful biocontrol agents discovered to date. Insect pathogenicity has also been attributed to strains of B. larvae, B. lentimorbus, B. sphaericus and B. cereus. Microbial insecticides, particularly those obtained from Bacillus strains, have played an important role in agriculture as alternatives to chemical pest control. Crop plants have been developed with enhanced insect resistance by genetically engineering crop plants to produce pesticidal proteins from Bacillus. For example, corn and cotton plants have been genetically engineered to produce pesticidal proteins isolated from strains of Bacillus thuringiensis. These genetically engineered crops are now widely used in agriculture and have provided the farmer with an environmentally friendly alternative to traditional insect-control methods. While they have proven to be very successful commercially, these genetically engineered, insect-resistant crop plants provide resistance to only a narrow range of the economically important insect pests. In some cases, insects can develop resistance to different insecticidal compounds, which raises the need to identify alternative biological control agents for pest control. Accordingly, there remains a need for new pesticidal proteins with different ranges of insecticidal activity against insect pests, e.g., insecticidal proteins which are active against a variety of insects in the order Lepidoptera and the order Coleoptera including but not limited to insect pests that have developed resistance to existing insecticides. SUMMARY In one aspect compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding sequences for pesticidal and insecticidal polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. Compositions also include the pesticidal polypeptide sequences and antibodies to those polypeptides. Compositions also comprise transformed bacteria, plants, plant cells, tissues and seeds. In another aspect isolated or recombinant nucleic acid molecules are provided encoding IPD082 polypeptides including amino acid substitutions, deletions, insertions, and fragments thereof. Provided are isolated or recombinant nucleic acid molecules capable of encoding IPD082 polypeptides of SEQ ID NO: 2, SEQ ID NO: 4 , SEQ ID NO: 6 , SEQ ID NO: 8 , SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26 or SEQ ID NO: 28 as well as amino acid substitutions, deletions, insertions, fragments thereof, and combinations thereof. Nucleic acid sequences that are complementary to a nucleic acid sequence of the embodiments or that hybridize to a sequence of the embodiments are also encompassed. The nucleic acid sequences can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. The nucleotide or amino acid sequences may be synthetic sequences that have been designed for expression in an organism including, but not limited to, a microorganism or a plant. In another aspect IPD082 polypeptides are encompassed. Also provided are isolated or recombinant IPD082 polypeptides of SEQ ID NO: 2, SEQ ID NO: 4 , SEQ ID NO: 6 , SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26 or SEQ ID NO: 28 as well as amino acid substitutions, deletions, insertions, fragments thereof and combinations thereof. In another aspect methods are provided for producing the polypeptides and for using those polypeptides for controlling or killing a Lepidopteran, Coleopteran, nematode, fungi, and/or Dipteran pests. The transgenic plants of the embodiments express one or more of the pesticidal sequences disclosed herein.
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