Details About Three Fatty Acid Oxidation Pathways Occurring in Man
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Abstract DRAYTON, JOSEPHINE BENTLEY
Abstract DRAYTON, JOSEPHINE BENTLEY. Methylated Medium- and Long-Chain Fatty Acids as Novel Sources of Anaplerotic Carbon for Exercising Mice. (Under the direction of Dr. Jack Odle and Dr. Lin Xi.) We hypothesized that methylated fatty acids (e.g. 2-methylpentanoic acid (2MeP), phytanic acid or pristanic acid) would provide a novel source of anaplerotic carbon and thereby enhance fatty acid oxidation, especially under stressed conditions when tricarboxylic acid (TCA) cycle intermediates are depleted. The optimal dose of 2MeP, hexanoic acid (C6) and pristanic acid for increasing in vitro [1-14C]-oleic acid oxidation in liver or skeletal muscle homogenates from fasted mice was determined using incremental doses of 0, 0.25, 0.5, 0.75, or 1.0 mM. The 0.25 mM amount maximally stimulated liver tissue oxidation of 14 14 14 [1- C]-oleate to CO2 and [ C]-acid soluble products (ASP; P < 0.05). Similar incubations of 0.25 mM 2MeP, C6, palmitate, phytanic acid, or pristanic acid or 0.1 mM malate or propionyl-CoA were conducted with liver or skeletal muscle homogenates from exercised or sedentary mice. In vitro oxidation of [1-14C]-oleic acid in liver homogenates with 2MeP 14 14 increased mitochondrial CO2 accumulation (P <0.05), but no change in [ C]-ASP accumulation as compared to C6 (P > 0.05). Phytanic acid treatment increased [14C]-ASP accumulation in liver tissue as compared to palmitate (P < 0.05). Exercise increased [14C] accumulations (P < 0.05). Results were consistent with our hypothesis that methyl-branched fatty acids (2-MeP, phytanic and pristanic acids) provide a novel source of anaplerotic carbon and thereby stimulate in vitro fatty acid oxidation in liver and skeletal muscle tissues. -
+1. Introduction 2. Cyrillic Letter Rumanian Yn
MAIN.HTM 10/13/2006 06:42 PM +1. INTRODUCTION These are comments to "Additional Cyrillic Characters In Unicode: A Preliminary Proposal". I'm examining each section of that document, as well as adding some extra notes (marked "+" in titles). Below I use standard Russian Cyrillic characters; please be sure that you have appropriate fonts installed. If everything is OK, the following two lines must look similarly (encoding CP-1251): (sample Cyrillic letters) АабВЕеЗКкМНОопРрСсТуХхЧЬ (Latin letters and digits) Aa6BEe3KkMHOonPpCcTyXx4b 2. CYRILLIC LETTER RUMANIAN YN In the late Cyrillic semi-uncial Rumanian/Moldavian editions, the shape of YN was very similar to inverted PSI, see the following sample from the Ноул Тестамент (New Testament) of 1818, Neamt/Нямец, folio 542 v.: file:///Users/everson/Documents/Eudora%20Folder/Attachments%20Folder/Addons/MAIN.HTM Page 1 of 28 MAIN.HTM 10/13/2006 06:42 PM Here you can see YN and PSI in both upper- and lowercase forms. Note that the upper part of YN is not a sharp arrowhead, but something horizontally cut even with kind of serif (in the uppercase form). Thus, the shape of the letter in modern-style fonts (like Times or Arial) may look somewhat similar to Cyrillic "Л"/"л" with the central vertical stem looking like in lowercase "ф" drawn from the middle of upper horizontal line downwards, with regular serif at the bottom (horizontal, not slanted): Compare also with the proposed shape of PSI (Section 36). 3. CYRILLIC LETTER IOTIFIED A file:///Users/everson/Documents/Eudora%20Folder/Attachments%20Folder/Addons/MAIN.HTM Page 2 of 28 MAIN.HTM 10/13/2006 06:42 PM I support the idea that "IA" must be separated from "Я". -
Cytochrome P450 Enzymes in Oxygenation of Prostaglandin Endoperoxides and Arachidonic Acid
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy 231 _____________________________ _____________________________ Cytochrome P450 Enzymes in Oxygenation of Prostaglandin Endoperoxides and Arachidonic Acid Cloning, Expression and Catalytic Properties of CYP4F8 and CYP4F21 BY JOHAN BYLUND ACTA UNIVERSITATIS UPSALIENSIS UPPSALA 2000 Dissertation for the Degree of Doctor of Philosophy (Faculty of Pharmacy) in Pharmaceutical Pharmacology presented at Uppsala University in 2000 ABSTRACT Bylund, J. 2000. Cytochrome P450 Enzymes in Oxygenation of Prostaglandin Endoperoxides and Arachidonic Acid: Cloning, Expression and Catalytic Properties of CYP4F8 and CYP4F21. Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from Faculty of Pharmacy 231 50 pp. Uppsala. ISBN 91-554-4784-8. Cytochrome P450 (P450 or CYP) is an enzyme system involved in the oxygenation of a wide range of endogenous compounds as well as foreign chemicals and drugs. This thesis describes investigations of P450-catalyzed oxygenation of prostaglandins, linoleic and arachidonic acids. The formation of bisallylic hydroxy metabolites of linoleic and arachidonic acids was studied with human recombinant P450s and with human liver microsomes. Several P450 enzymes catalyzed the formation of bisallylic hydroxy metabolites. Inhibition studies and stereochemical analysis of metabolites suggest that the enzyme CYP1A2 may contribute to the biosynthesis of bisallylic hydroxy fatty acid metabolites in adult human liver microsomes. 19R-Hydroxy-PGE and 20-hydroxy-PGE are major components of human and ovine semen, respectively. They are formed in the seminal vesicles, but the mechanism of their biosynthesis is unknown. Reverse transcription-polymerase chain reaction using degenerate primers for mammalian CYP4 family genes, revealed expression of two novel P450 genes in human and ovine seminal vesicles. -
Mechanistic Studies on Medium Chain Acyl-Coa Dehydrogenase
Acyl-CoA Dehydrogenases: Mechanistic studies on Medium Chain Acyl-CoA Dehydrogenase Dissertation zur Erlangung des akademischen Grades des Doktors der Naturwissenschaften (Dr. rer. nat.) an der Universität Konstanz (Fachbereich Biologie) vorgelegt von Vasile Robert Gradinaru Konstanz, im Juni 2005 Tag der mündlichen Prüfung : 9. November 2005 Referent: Prof. Dr. Sandro Ghisla Referent: Prof. Dr. Peter Macheroux Acknowledgements I am greatly indebted to my supervisor, Prof. Dr. Sandro Ghisla, for his advice and support. He has been an excellent supervisor providing insightful comments and constructive criticism throughout this PhD project. I would also like to thank my colleagues in the Universities of Konstanz and Iasi for their advice, encouragement and friendship, without which I would certainly not have completed this thesis. In particular, I would like to thank Prof. Dr. Richard Schowen, Prof. Dr. Peter Macheroux, Prof. Dr. Colin Thorpe, Prof. Dr. Jung-Ja. Kim, Prof. Dr. Tatiana Nicolaescu, Prof. Dr. Constantin Ciugureanu, Prof. Dr. Robert Bach, Dr. Olga Dmitrenko, Susanne Feindler-Boeckh, Gudrun Vogt, Elmi Leisner, Karl Janko, Lili Smau, Nasser Ibrahim, Phaneeswara Rao Kommoju, Sudarshan Rao Ande, Lakshminarayana Kaza, Cosmin Pocanschi, Paula Bulieris for their active interest during the course of this project. My gratitude also goes to Prof. Alexandru Cecal for encouraging me to embark on this PhD. I express my loving thanks to my wife Luiza whose love, support, patience and understanding made this work easier. Above all, I wish to express my sincerest gratitude to my parents, who made my studies possible and who have always encouraged me. This study was financiarlly supported by the Deutsche Forschungsgemeinschaft (Gh 2/6-4). -
Substrate Specificity of Rat Liver Mitochondrial Carnitine Palmitoyl Transferase I: Evidence Against S-Oxidation of Phytanic Acid in Rat Liver Mitochondria
FEBS 15107 FEBS Letters 359 (1995) 179 183 Substrate specificity of rat liver mitochondrial carnitine palmitoyl transferase I: evidence against s-oxidation of phytanic acid in rat liver mitochondria Harmeet Singh*, Alfred Poulos Department of Chemical Pathology, Women's and Children's Hospital 72 King William Road, North Adelaide, SA 5006, Australia Received 23 December 1994 demonstrated that CPTI activity is reversibly inhibited by Abstract The two branched chain fatty acids pristanic acid malonyl-CoA. Recently, it has been shown that malonyl-CoA (2,6,10,14-tetramethylpentadecanoic acid) and phytanic acid inhibits peroxisomal, microsomal and plasma membrane car- (3,7,11,15-tetramethylhexadecanoic acid) were converted to co- nitine acyl transferases [8,10,13]. Therefore, in order to under- enzyme A thioesters by rat liver mitochondrial outer membranes. However, these branched chain fatty acids could not be converted stand the role of CPTI in fatty acid oxidation, CPTI must be to pristanoyl and phytanoyl carnitines, respectively, by mitochon- separated from other carnitine acyl transferases. In view of the drial outer membranes. As expected, the unbranched long chain difficulties in isolating CPTI in the active state, we decided to fatty acids, stearic acid and palmitic acid, were rapidly converted investigate CPTI activity in highly purified mitochondrial outer to stearoyl and palmitoyl carnitines, respectively, by mitochon- membranes from rat liver. We present evidence that branched drial outer membranes. These observations indicate that the chain fatty acids with ~- or fl-methyl groups are poor substrates branched chain fatty acids could not be transported into mito- for CPTI, suggesting that these branched chain fatty acids chondria. -
Inborn Errors of Metabolism Test Requisition
LABORATORY OF GENETICS AND GENOMICS Mailing Address: For local courier service and/or inquiries, please contact 513-636-4474 • Fax: 513-636-4373 3333 Burnet Avenue, Room R1042 www.cincinnatichildrens.org/moleculargenetics • Email: [email protected] Cincinnati, OH 45229 INBORN ERRORS OF METABOLISM TEST REQUISITION All Information Must Be Completed Before Sample Can Be Processed PATIENT INFORMATION ETHNIC/RACIAL BACKGROUND (Choose All) Patient Name: ___________________ , ___________________ , ________ European American (White) African-American (Black) Last First MI Native American or Alaskan Asian-American Address: ____________________________________________________ Pacific Islander Ashkenazi Jewish ancestry ____________________________________________________ Latino-Hispanic _____________________________________________ Home Phone: ________________________________________________ (specify country/region of origin) MR# __________________ Date of Birth ________ / ________ / _______ Other ____________________________________________________ (specify country/region of origin) Gender: Male Female BILLING INFORMATION (Choose ONE method of payment) o REFERRING INSTITUTION o COMMERCIAL INSURANCE* Insurance can only be billed if requested at the time of service. Institution: ____________________________________________________ Policy Holder Name: _____________________________________________ Address: _____________________________________________________ Gender: ________________ Date of Birth ________ / ________ / _______ -
Colorectal Cancer and Omega Hydroxylases
1 The differential expression of omega-3 and omega-6 fatty acid metabolising enzymes in colorectal cancer and its prognostic significance Abdo Alnabulsi1,2, Rebecca Swan1, Beatriz Cash2, Ayham Alnabulsi2, Graeme I Murray1 1Pathology, School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, AB25, 2ZD, UK. 2Vertebrate Antibodies, Zoology Building, Tillydrone Avenue, Aberdeen, AB24 2TZ, UK. Address correspondence to: Professor Graeme I Murray Email [email protected] Phone: +44(0)1224 553794 Fax: +44(0)1224 663002 Running title: omega hydroxylases and colorectal cancer 2 Abstract Background: Colorectal cancer is a common malignancy and one of the leading causes of cancer related deaths. The metabolism of omega fatty acids has been implicated in tumour growth and metastasis. Methods: This study has characterised the expression of omega fatty acid metabolising enzymes CYP4A11, CYP4F11, CYP4V2 and CYP4Z1 using monoclonal antibodies we have developed. Immunohistochemistry was performed on a tissue microarray containing 650 primary colorectal cancers, 285 lymph node metastasis and 50 normal colonic mucosa. Results: The differential expression of CYP4A11 and CYP4F11 showed a strong association with survival in both the whole patient cohort (HR=1.203, 95% CI=1.092-1.324, χ2=14.968, p=0.001) and in mismatch repair proficient tumours (HR=1.276, 95% CI=1.095-1.488, χ2=9.988, p=0.007). Multivariate analysis revealed that the differential expression of CYP4A11 and CYP4F11 was independently prognostic in both the whole patient cohort (p = 0.019) and in mismatch repair proficient tumours (p=0.046). Conclusions: A significant and independent association has been identified between overall survival and the differential expression of CYP4A11 and CYP4F11 in the whole patient cohort and in mismatch repair proficient tumours. -
ACADS Rabbit Pab
Leader in Biomolecular Solutions for Life Science ACADS Rabbit pAb Catalog No.: A0945 2 Publications Basic Information Background Catalog No. This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the A0945 acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been Observed MW associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative 44kDa splicing results in two variants which encode different isoforms. Calculated MW 44kDa Category Primary antibody Applications WB, IHC, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 35 P16219 IHC 1:50 - 1:100 Immunogen 1:50 - 1:200 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ACADS (NP_000008.1). Synonyms ACADS;ACAD3;SCAD Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using ACADS antibody (A0945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunofluorescence analysis of U2OS embedded human liver cancer using embedded human liver damage using cells using ACADS antibody (A0945). Blue: ACADS antibody (A0945) at dilution of ACADS antibody (A0945) at dilution of DAPI for nuclear staining. -
Old Cyrillic in Unicode*
Old Cyrillic in Unicode* Ivan A Derzhanski Institute for Mathematics and Computer Science, Bulgarian Academy of Sciences [email protected] The current version of the Unicode Standard acknowledges the existence of a pre- modern version of the Cyrillic script, but its support thereof is limited to assigning code points to several obsolete letters. Meanwhile mediæval Cyrillic manuscripts and some early printed books feature a plethora of letter shapes, ligatures, diacritic and punctuation marks that want proper representation. (In addition, contemporary editions of mediæval texts employ a variety of annotation signs.) As generally with scripts that predate printing, an obvious problem is the abundance of functional, chronological, regional and decorative variant shapes, the precise details of whose distribution are often unknown. The present contents of the block will need to be interpreted with Old Cyrillic in mind, and decisions to be made as to which remaining characters should be implemented via Unicode’s mechanism of variation selection, as ligatures in the typeface, or as code points in the Private space or the standard Cyrillic block. I discuss the initial stage of this work. The Unicode Standard (Unicode 4.0.1) makes a controversial statement: The historical form of the Cyrillic alphabet is treated as a font style variation of modern Cyrillic because the historical forms are relatively close to the modern appearance, and because some of them are still in modern use in languages other than Russian (for example, U+0406 “I” CYRILLIC CAPITAL LETTER I is used in modern Ukrainian and Byelorussian). Some of the letters in this range were used in modern typefaces in Russian and Bulgarian. -
Ataxia with Loss of Purkinje Cells in a Mouse Model for Refsum Disease
Ataxia with loss of Purkinje cells in a mouse model for Refsum disease Sacha Ferdinandussea,1,2, Anna W. M. Zomerb,1, Jasper C. Komena, Christina E. van den Brinkb, Melissa Thanosa, Frank P. T. Hamersc, Ronald J. A. Wandersa,d, Paul T. van der Saagb, Bwee Tien Poll-Thed, and Pedro Britesa Academic Medical Center, Departments of aClinical Chemistry (Laboratory of Genetic Metabolic Diseases) and dPediatrics, Emma’s Children Hospital, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; bHubrecht Institute, Royal Netherlands Academy of Arts and Sciences 3584 CT Utrecht, The Netherlands; and cRehabilitation Hospital ‘‘De Hoogstraat’’ Rudolf Magnus Institute of Neuroscience, 3584 CG Utrecht, The Netherlands Edited by P. Borst, The Netherlands Cancer Institute, Amsterdam, The Netherlands, and approved October 3, 2008 (received for review June 23, 2008) Refsum disease is caused by a deficiency of phytanoyl-CoA hy- Clinically, Refsum disease is characterized by cerebellar droxylase (PHYH), the first enzyme of the peroxisomal ␣-oxidation ataxia, polyneuropathy, and progressive retinitis pigmentosa, system, resulting in the accumulation of the branched-chain fatty culminating in blindness, (1, 3). The age of onset of the symptoms acid phytanic acid. The main clinical symptoms are polyneuropathy, can vary from early childhood to the third or fourth decade of cerebellar ataxia, and retinitis pigmentosa. To study the patho- life. No treatment is available for patients with Refsum disease, genesis of Refsum disease, we generated and characterized a Phyh but they benefit from a low phytanic acid diet. Phytanic acid is knockout mouse. We studied the pathological effects of phytanic derived from dietary sources only, specifically from the chloro- acid accumulation in Phyh؊/؊ mice fed a diet supplemented with phyll component phytol. -
BMC Genomics Biomed Central
BMC Genomics BioMed Central Research article Open Access Differential gene expression in femoral bone from red junglefowl and domestic chicken, differing for bone phenotypic traits Carl-Johan Rubin1, Johan Lindberg2, Carolyn Fitzsimmons3, Peter Savolainen2, Per Jensen4, Joakim Lundeberg2, Leif Andersson3,5 and Andreas Kindmark*1 Address: 1Department of Medical Sciences, Uppsala University, Sweden, 2Department of Gene Technology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden, 3Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden, 4IFM Biology, Linköping University, SE-585 83, Linköping, Sweden and 5Department of Medical Biochemistry and Microbiology, Uppsala University, Box 597, SE-75124 Uppsala, Sweden Email: Carl-Johan Rubin - [email protected]; Johan Lindberg - [email protected]; Carolyn Fitzsimmons - [email protected]; Peter Savolainen - [email protected]; Per Jensen - [email protected]; Joakim Lundeberg - [email protected]; Leif Andersson - [email protected]; Andreas Kindmark* - [email protected] * Corresponding author Published: 2 July 2007 Received: 2 April 2007 Accepted: 2 July 2007 BMC Genomics 2007, 8:208 doi:10.1186/1471-2164-8-208 This article is available from: http://www.biomedcentral.com/1471-2164/8/208 © 2007 Rubin et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Osteoporosis is frequently observed among aging hens from egg-producing strains (layers) of domestic chicken. -
The Role of Alpha Oxidation in Lipid Metabolism, 2017
THE ROLE OF ALPHA OXIDATION IN LIPID METABOLISM Benjamin John Jenkins Darwin College Medical Research Council – Human Nutrition Research Department of Biochemistry University of Cambridge This dissertation is submitted for the degree of Doctor of Philosophy July 2018 DECLARATION This dissertation is the result of my own work and includes nothing, which is the outcome of work done in collaboration except as declared in the preface and specified in the text. It is not substantially the same as any that I have submitted, or, is being concurrently submitted for a degree or diploma or other qualification at the University of Cambridge or any other University or similar institution except as declared in the Preface and specified in the text. I further state that no substantial part of my dissertation has already been submitted, or, is being concurrently submitted for any such degree, diploma or other qualification at the University of Cambridge or any other University or similar institution except as declared in the Preface and specified in the text. In accordance with the University of Cambridge guidelines, this thesis does not exceed 60,000 words. Signed: ______________________________________________________________ Date: _______________________________________________________________ Benjamin John Jenkins BSc. MSc. Darwin College, Silver Street, Cambridge, CB3 9EU by i 2014 Word Template Template Friedman Friedman & Morgan Morgan & ii The Role of Alpha Oxidation in Lipid Metabolism, 2017 ABSTRACT Recent findings have shown an inverse association between the circulating levels of pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0) with the risk of pathological development in type 2 diabetes, cardio vascular disease and neurological disorders. From previously published research, it has been said that both these odd chain fatty acids are biomarkers of their dietary intake and are significantly correlated to dietary ruminant fat intake.