Product datasheet Anti-MIEF1 Antibody Catalog Number: A06887-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-MIEF1 Antibody Name MIEF1 Source Rabbit IgG Species Reactivity human, mouse, rat Tested Application WB,IHC-P,ICC/IF,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human MIEF1 recombinant (Position: S189-T463). Purification Immunogen affinity purified. Observed MW 51KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section IHC-(P)： 1:50-400 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Mitochondrial dynamic protein MID51 (MID51) also known as mitochondrial elongation factor 1 (MIEF1) or Smith-Magenis syndrome region candidate gene 7 protein-like (SMCR7L) is a protein that in humans is encoded by the SMCR7L gene. The SMCR7L gene codes for a protein that has been called MiD51/MIEF1 and shown to regulate mitochondrial fission by interacting with the Drp1 and FIS1.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-MIEF1 Antibody Catalog Number: A06887-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. IHC analysis analysis of anti- MIEF1 using anti- MIEF1 antibody (A06887-1). The antibody (A06887-1). sample well of each lane detected in paraffin- was loaded with 50ug of embedded section of sample under reducing human renal clear cell conditions. Lane 1: carcinoma tissue. human HT1080 whole cell Biotinylated goat anti- lysates, Lane 2: human rabbit IgG was used as Jurkat whole cell lysates, secondary antibody. The Lane 3: human K562 tissue section was whole cell lysates, Lane 4: developed using Strepavi human HL-60 whole cell din-Biotin-Complex lysates, Lane 5: human (SABC) (Catalog # HEK293 whole cell SA1022) with DAB as the lysates, Lane 6: human chromogen. U937 whole cell lysates, Lane 7: human HEPG2 whole cell lysates, Lane 8: rat heart tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: mouse kidney tissue lysates. Use rabbit anti- MIEF1 1:1000, probed with a goat anti- rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MIEF1 at approximately 51KD. The expected band size for MIEF1 is at 51KD.

Figure 3. IHC analysis Figure 4. ICC analysis using anti- MIEF1 using anti- MIEF1 antibody (A06887-1). antibody (A06887-1). was detected in paraffin- detected in immersion embedded section of fixed U2OS cell line. Cells human rectal cancer were stained using the tissue. Biotinylated goat Dylight488-conjugated anti-rabbit IgG was used Anti-rabbit IgG as secondary antibody. Secondary Antibody The tissue section was (green)Catalog # BA1127)

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-MIEF1 Antibody Catalog Number: A06887-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

developed using Strepavi and counterstained with din-Biotin-Complex DAPI (blue). (SABC) (Catalog # SA1022) with DAB as the chromogen.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3

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