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Dissertation LFS Corrected 08-22-16 EXPRESSION AND ROLE OF FIBROBLAST GROWTH FACTOR 9 (FGF9) IN BOVINE FOLLICULOGENESIS By LUÍS FERNANDO SCHÜTZ Doctor of Veterinary Medicine Federal University of Rio Grande do Sul Porto Alegre, Brazil 2007 Master of Science in Animal Science Santa Catarina State University Lages, Brazil Submitted to the Faculty of the Graduate College of the Oklahoma State University in partial fulfillment of the requirements for the Degree of DOCTOR OF PHILOSOPHY July, 2016 EXPRESSION AND ROLE OF FIBROBLAST GROWTH FACTOR 9 (FGF9) IN BOVINE FOLLICULOGENESIS Dissertation Approved: Dr. Leon J. Spicer Dissertation Adviser Dr. Robert Wettemann Dr. Glenn Zhang Dr. Peter Hoyt ii ACKNOWLEDGEMENTS To my beloved wife, for her love, support, encouragement, and understanding. She made all the steps of the arduous pathway to achieve this degree much easier. With her by my side, I feel much stronger. To my family in Brazil: my beloved parents, my brother, Lipe, and my sister, Ane, for their love, faith, and support. And to my beautiful niece, Sofia, my sister-in-law, Manuela, and my brother-in-law, Joni. To my advisor and mentor, Dr. Leon Spicer, for accepting me as a student, for giving me priceless lessons and advice whenever I needed, for stimulating me to develop a critical thinking, and for teaching me how to perform sound science in the field of ovarian folliculogenesis. And to his family, Michael, Melissa, Anna, and Richie, for their friendship and support. To the Franceschi family: my mother-in-law, Pierina, and my father-in-law, Adi, for their support and encouragement; to my brother-in-law, Junior, his wife, Janine, and his great boys, Bernardo and Bruno; and to my sister-in-law, Sheila, and her fiancé, Fábio. To my committee members, Dr. Robert Wettemann, Dr. Peter Hoyt, and Dr. Glenn Zhang for their teaching and support. To the Faculty of Animal Science Department at OSU, specially Dr. Clint Rusk, Dr. Jennifer Hernandez-Gifford, and Dr. Craig Gifford, who always have been very supportive. To all my friends in Brazil, who always encourage me and make my life happier just because I know they will always be there for me. To the friends and colleagues I met in OSU, especially Marco Albonico, Lingna Zhang, Britt Boehmer, Bahaa Aloqaily, Pauline Aad, Breanne Morell, Belinda and Renee iii Acknowledgements reflect the views of the author and are not endorsed by committee members or Oklahoma State University. Gomez, Cheyenne Robinson, Maria Chiara Perego, Denise Tsuzukibashi, Morgan Totty, Patrick Jones, Phillip Gunter, and Kyle McLean. Their friendship and support surely made my work and my life in America much more pleasant. To the Brazilian friends that I have met in Stillwater, especially Rolf and Bia, Tony and Thaís, Samuel and Tathiane, among many others. To my friends and advisors, Dr. Marcelo Bertolini and Dr. Valério Portela, for their support and encouragement. To my grandparents, who are no longer with me on earth, but from whom I carry not just my genetic and epigenetic heritage, but everlasting love. To God, who has always blessed my life, providing me opportunities to meet great people wherever I go. iv Acknowledgements reflect the views of the author and are not endorsed by committee members or Oklahoma State University. Name: LUÍS FERNANDO SCHÜTZ Date of Degree: JULY, 2016 Title of Study: EXPRESSION AND ROLE OF FIBROBLAST GROWTH FACTOR 9 (FGF9) IN BOVINE FOLLICULOGENESIS Major Field: ANIMAL SCIENCE Abstract: In the ovaries, many members of the FGF family are involved in the regulation of folliculogenesis. One of these members, FGF9, has been characterized as an anti-differentiation factor for its effects on steroidogenesis of granulosa (GC) and theca cells (TC) of cattle, but whether endogenous production of FGF9 changes during follicular development of cattle was unknown. Also, in order to have a comprehensive view of how FGFs regulate folliculogenesis, information of how endogenous production of their receptors (FGFRs) is also required. The first objectives of our studies presented herein was to characterize FGF9, FGFR1c, FGFR2c, FGFR3c, and FGFR4 mRNA abundance in GC and TC during development of dominant follicles in dairy cattle. Estrous cycles of non-lactating dairy cattle were synchronized, and ovaries were collected on either day 3-4 (n = 8) or day 5-6 (n = 8) post-ovulation for GC and TC mRNA extraction from small (1-5 mm), medium (5.1-8 mm) or large (8.1-18 mm) follicles for PCR analysis. In GC, FGF9, FGFR1c, FGFR2c, and FGFR3c mRNA abundance was greater (P < 0.05) in estrogen (E2)- inactive (i.e., concentrations of E2 < progesterone, P4) follicles than in large E2- active (i.e., concentrations of E2 > P4) follicles. In TC, FGF9 mRNA abundance was greater (P < 0.05) in large E2-inactive follicles than in large E2-active follicles on day 3-4 post-ovulation whereas medium E2-inactive follicles have greater (P < 0.05) FGFR1c and FGFR4 mRNA abundance than large E2-active and E2–inactive follicles on day 5-6 post-ovulation. In addition, FGFR1c, FGFR2c, FGFR3c, and FGFR4 mRNA abundance was greater (P < 0.05) in medium E2-inactive follicles on day 5-6 post-ovulation than on day 3-4 post-ovulation. Following, we aimed to investigate effects of FGF9 on TC through the use of microarray (Affymetrix GeneChip Bovine Genome Arrays) and the bioinformatic tool Ingenuity Pathway Analysis. This software identified differentially expressed transcripts in 346 pathways in response to FGF9 in TC involved in functions such as cell cycle, proliferation, survival, and steroidogenesis. Taken together, these studies reinforce the importance of FGFs in folliculogenesis of cattle. v TABLE OF CONTENTS Chapter Page I. INTRODUCTION ......................................................................................................1 II. REVIEW OF LITERATURE ....................................................................................4 1. Fibroblast growth factors family of proteins and their receptors .........................4 2. Role of fibroblast growth factors on ovarian physiology ....................................7 2.1. Role of fibroblast growth factors and gonadotropins on ovarian steroidogenesis ................................................................................................................................10 2.2. Role of fibroblast growth factors in proliferation of granulosa and theca cells................................. .......................................................................................13 2.3. Role of fibroblast growth factors in ovarian follicular fate ..........................15 2.4. Role of fibroblast growth factors in luteal function ......................................17 2.5. Role of fibroblast growth factor receptors on ovarian physiology ...............18 3. Role of other growth factors on ovarian physiology .........................................21 3.1. IGF system ....................................................................................................22 3.2. Transforming growth factor β family ...........................................................27 3.2.1. Role of TGF-α and TGF-β on folliculogenesis ......................................28 3.2.2. Role of activin, inhibin, and follistatin on folliculogenesis ...................29 3.2.3. Role of oocyte-derived GDF-9, BMP-6, and BMP-15 on folliculogenesis ................................................................................................................................31 3.2.4. Role of BMPs on folliculogenesis .........................................................33 3.2.5. Role of AMH on folliculogenesis ..........................................................34 4. Bioinformatics tools for microarray analysis .....................................................35 5. References .........................................................................................................41 III. CHANGES IN FIBROBLAST GROWTH FACTOR 9 (FGF9) mRNA IN GRANULOSA AND THECA CELLS DURING OVARIAN FOLLICULAR GROWTH IN DAIRY CATTLE ...........................................................................91 1. Abstract ..............................................................................................................91 2. Introduction ........................................................................................................92 3. Material and methods .........................................................................................93 Animals and Experimental Designs .................................................................93 Cell and Follicular Fluid Collection ................................................................94 RNA Extraction and Qualitative PCR (qPCR) ................................................94 Radioimmunoassays (RIA) and IGF1 ELISA .................................................95 FGF9 Western Blotting ....................................................................................96 vi Statistical Analyses ..........................................................................................97 4. Results ................................................................................................................98 GC FGF9 mRNA Abundance ..........................................................................98 TC FGF9 mRNA Abundance ..........................................................................98 Follicle Size, E2, P4, and Free IGF1 Concentrations in Follicular Fluid ........99 vi Correlations between FFL Concentrations of Hormones
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