Redalyc.LACCASE ACTIVITY of Pycnoporus Cinnabarinus GROWN in DIFFERENT CULTURE SYSTEMS
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Revista Mexicana de Ingeniería Química ISSN: 1665-2738 [email protected] Universidad Autónoma Metropolitana Unidad Iztapalapa México Villegas, E.; Téllez-Téllez, M.; Rodríguez, A.; Carreón-Palacios, A.E.; Acosta-Urdapilleta, M.L.; Kumar-Gupta, V.; Díaz-Godínez, G. LACCASE ACTIVITY OF Pycnoporus cinnabarinus GROWN IN DIFFERENT CULTURE SYSTEMS Revista Mexicana de Ingeniería Química, vol. 15, núm. 3, 2016, pp. 703-710 Universidad Autónoma Metropolitana Unidad Iztapalapa Distrito Federal, México Available in: http://www.redalyc.org/articulo.oa?id=62048168003 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Vol. 15, No. 3 (2016) 703-710 Revista Mexicana de Ingeniería Química LACCASE ACTIVITY OF PycnoporusCONTENIDO cinnabarinus GROWN IN DIFFERENT CULTURE SYSTEMS ACTIVIDADVolumen DE LACASA 8, número 3, DE 2009Pycnoporus / Volume 8, cinnabarinusnumber 3, 2009CRECIDO EN DIFERENTES SISTEMAS DE CULTIVO E. Villegas2, M. Tellez-T´ ellez´ 3, A. Rodr´ıguez2, A.E. Carreon-Palacios´ 1, M.L. Acosta-Urdapilleta3, 213 Derivation and applicationV. Kumar-Guptaof the Stefan-Maxwell4, G. Dequations´ıaz-God ´ınez1* 1Laboratory of Biotechnology, Research Center for Biological Sciences, Autonomous University of Tlaxcala, Tlaxcala, Mexico. 2Laboratory of Structure-Function (Desarrollo y aplicación and Protein de las Engineering, ecuaciones de Biotechnology Stefan-Maxwell) Research Center of the Autonomous University of Stephen Whitaker the State of Morelos, Cuernavaca, Morelos. 3 Laboratory of Mycology, Biology Research Center, Autonomous University of the State of Morelos, Cuernavaca, Morelos. 4Molecular Glycobiotechnology Group, Discipline of Biochemistry, National University of Ireland Galway, Galway, Ireland. Biotecnología / BiotechnologyReceived March 10, 2016; Accepted May 30, 2016 245 Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo Abstract Activity and zymogramintemperizados patterns ofen suelos laccases y sedimentos produced by Pycnoporus cinnabarinus (HEMIM-79) grown in three systems of submerged fermentation (Biodegradation (airlift modeling reactor, stirred of sludge tank bioreactors reactor and of Erlenmeyertotal petroleum flasks) hydrocarbons and in solid-state weathering fermentation in soil using polyurethane foam as inert support, were studied. A culture medium based in glucose, yeast extract and mineral salts and sediments) including copper sulfate as laccases inductor, was used in all grown systems. Very different activity levels were observed depending on the growthS.A. Medina-Moreno, system and inS. allHuerta-Ochoa, cases the zymogram C.A. Lucho-Constantino, patterns were similar.L. Aguilera-Vázquez, So, in this strain,A. Jiménez- the number of isoenzymes was independentGonzález y M. of Gutiérrez-Rojas culture conditions but their amount changed, showing the highest values in the culture in shake flasks followed259 Crecimiento, by the solid-state sobrevivencia fermentation, y adaptación followed de Bifidobacterium by stirred tank infantis reactor a condiciones and the lowest ácidas activity was observed in airlift reactor. It is suggested that the difference in laccase activity could be due to the type of growth given in each system, the pellets (Growth, by their survival small size and areadaptation the metabolically of Bifidobacterium more activeinfantis compared to acidic conditions) to the big mass produced in the airlift reactor; although theL. stirredMayorga-Reyes, tank showed P. Bustamante-Camilo, small pieces of mycelium, A. Gutiérrez-Nava, not showed E. an Barranco-Florido adequate growth byy A. mechanical Azaola- damage to cells. In the caseEspinosa of solid culture, the fungus growth was in mycelium form which is similar to its natural habitat. Keywords: airlift265 reactor, Statistical Erlenmeyer approach flasks,to optimization isoenzymes, of ethanol laccases, fermentation solid-state by fermentation, Saccharomyces stirred cerevisiae tank reactor. in the Resumen presence of Valfor® zeolite NaA Se obtuvo la actividad y los patrones zimograficos´ de lacasas producidas por Pycnoporus cinnabarinus (HEMIM-79) crecido en tres sistemas(Optimización de fermentaci estadísticaon´ sumergida de la fermentación (reactor airlift,etanólica reactor de Saccharomyces de tanque agitado cerevisiae y matraces en presencia Erlenmeyer) de y en fermentacion´ en estadozeolita solido´ Valfor® usando zeolite espuma NaA) de poliuretano como soporte inerte. En todos los sistemas se utilizo´ un medio de cultivo con glucosa, extracto de levadura, sales minerales y sulfato de cobre como inductor de lacasas. Se observaron G. Inei-Shizukawa, H. A. Velasco-Bedrán, G. F. Gutiérrez-López and H. Hernández-Sánchez diferentes niveles de actividad en funcion´ del sistema de crecimiento y en todos los casos los patrones de zimograf´ıa fueron similares. Por lo tanto, en esta cepa, el numero´ de isoenzimas fue independiente de las condiciones de cultivo, pero la cantidad cambiIngenieríao,´ mostrando de procesos los valores / Process mas´ engineering altos en el cultivo en matraz agitado, seguido de la fermentacion´ en estado solido,´ luego del271 reactor Localización de tanque de una agitado, planta la industrial: actividad mRevisiónas´ baja secrítica observ y adecuacióno´ en el reactor de los airlift. criterios Se sugiereempleados que en la diferencia en actividad de lacasaesta podrdecisión´ıa deberse al tipo de crecimiento dado en cada sistema, siendo los pellets por su pequeno˜ tamano˜ los metabolicamente mas´ activos en comparacion´ con la gran masa producida en el reactor airlift y el tanque agitado aunque (Plant site selection: Critical review and adequation criteria used in this decision) mostro´ pequenos˜ trozos de micelio, no hubo un adecuado crecimiento por el dano˜ mecanico´ causado a las celulas.´ En el J.R. Medina, R.L. Romero y G.A. Pérez caso del cultivo solido,´ el hongo se desarrollo´ en forma de micelio el cual es lo mas parecido a su habitat natural. Palabras clave : reactor airlift, matraces Erlenmeyer, isoenzimas, lacasas, fermentacion´ en estado solido,´ reactor de tanque agitado. * Corresponding author. E-mail: [email protected] Tel/Fax. 248-48-154-82 Publicado por la Academia Mexicana de Investigacion´ y Docencia en Ingenier´ıa Qu´ımica A.C. 703 Villegas et al./ Revista Mexicana deIngenier ´ıaQu ´ımica Vol. 15, No. 3 (2016) 703-710 1 Introduction various substrates, indicating that laccase isoforms were specific for each species. The differences Lignin is a complex and heterogeneous aromatic observed in number and position of isoforms in the biopolymer which provides strength to the wood gel, suggest that laccase zymograms might be a structure and protects it against microbial attack. way to differentiate species of this genus. In other The most effective lignin degraders in nature are the study, the cultures of Pleurotus ostreatus grown in white-rot fungi which belong to the basidiomycetes submerged fermentation produced laccase at 13,000 (Ander and Eriksson, 1978). Enzymes involved in UL−1, with a biomass production of 5.6 g L−1 and the degradation of lignin oxidize phenolic structures four laccase isoforms, however, cultures grown in and catalyze the generation of highly reactive solid-state fermentation had a much lower laccase radicals that can also degrade non phenolic structures activity of 2,430 U L−1, biomass production of 4.5 (Boominathan and Reddy, 1992; Thurston, 1994). g L−1, and three laccase isoforms (Tellez-T´ ellez´ et The three classes of extracellular lignin modifying al., 2008). D´ıaz et al. (2013) reported that the enzymes generally recognized are lignin peroxidase, initial pH of the growing medium is an important Mn dependent peroxidase and laccase. White- factor for regulating the expression of laccase genes rot fungi produce one, two or the three of these which have an effect on the activity and number of phenoloxidase enzymes and this appears to be a laccase isoenzymes produced by Pleurotus ostreatus feature among this group of fungi (Orth et al., 1993). in submerged fermentation (SmF). Laccase (ρ-diphenol: oxygen oxidoreductases) was Guzman´ (2003) considers that Pycnoporus first demonstrated in the exudates of Rhus vernicifera, sanguineus is a tropical variant of Pycnoporus the Japanese lacquer tree (Yoshida, 1883). Later it cinnabarinus from the temperate zone, adapted to was demonstrated in fungi (Bertrand, 1896). Laccases, man disturbed sites, where it is common in fallen are either mono or multimeric copper-containing logs and fences, always in sunny places. It is oxidases that catalyze the reduction of oxygen to closely related species, Pycnoporus coccineus, and water accompanied by the oxidation of a phenolic Pycnoporus sanguineus. These fungi are recognized substrates. Molecular oxygen serves as the terminal as efficient lignin decomposers, in spite of its relatively electron acceptor and is thus reduced to two molecules simple lignin modifying enzyme system composed of of water, as one electron oxidation of a substrate is laccases (Eggert et al., 1996). These features make coupled to a four-electron reduction of oxygen, the Pycnoporus species an attractive group of white-rot reaction mechanism cannot be straight forward. When basidiomycetes for the production and purification of oxidized by a laccase, the reducing substrate loses laccases.