Histone H1t Rabbit Pab

Total Page:16

File Type:pdf, Size:1020Kb

Histone H1t Rabbit Pab Leader in Biomolecular Solutions for Life Science Histone H1t Rabbit pAb Catalog No.: A18496 Basic Information Background Catalog No. Histones are basic nuclear proteins responsible for nucleosome structure of the A18496 chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is Observed MW wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with 30kDa linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent Calculated MW histone that is a member of the histone H1 family. Transcripts from this gene lack polyA 22kDa tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. Category Primary antibody Applications WB, IHC Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 3010 P22492 IHC 1:50 - 1:200 Immunogen A synthetic peptide corresponding to a sequence within amino acids 150-207 of human Histone H1t (NP_005314.2). Synonyms H1.6;H1FT;H1t;dJ221C16.2;HIST1H1T Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using Histone H1t antibody (A18496) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded human esophageal using embedded mouse kidney using Histone embedded rat lung using Histone H1t Histone H1t Rabbit pAb (A18496) at H1t Rabbit pAb (A18496) at dilution of Rabbit pAb (A18496) at dilution of 1:100 dilution of 1:100 (40x lens). 1:100 (40x lens). (40x lens). Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..
Recommended publications
  • Co-Occurrence Based Meta-Analysis of Scientific Texts
    Vol. 21 no. 9 2005, pages 2049–2058 BIOINFORMATICS ORIGINAL PAPER doi:10.1093/bioinformatics/bti268 Data and text mining Co-occurrence based meta-analysis of scientific texts: retrieving biological relationships between genes R. Jelier1,∗ G. Jenster2, L. C. J. Dorssers3, C. C. van der Eijk1, E. M. van Mulligen1, B. Mons1 and J. A. Kors1 1Department of Medical Informatics, 2Department of Urology and 3Department of Pathology, Erasmus MC—University Medical Center, Rotterdam, The Netherlands Received on July 15, 2004; revised on December 22, 2004; accepted on January 6, 2005 Advance Access publication January 18, 2005 ABSTRACT are studied, the number of relevant publications will frequently be Motivation: The advent of high-throughput experiments in molecu- prohibitively large. This renders the traditional approach of manu- lar biology creates a need for methods to efficiently extract and use ally searching bibliographic databases for every gene and reading information for large numbers of genes. Recently, the associative scientific articles inadequate. It is therefore an important challenge concept space (ACS) has been developed for the representation of at this time to make the available information both accessible and information extracted from biomedical literature. The ACS is a Euc- interpretable for molecular biologists. lidean space in which thesaurus concepts are positioned and the An interesting current development is the use of annotations of distances between concepts indicates their relatedness. The ACS genes with gene ontology (GO) terms (Ashburner et al., 2000; Camon uses co-occurrence of concepts as a source of information. In this et al., 2004) for the analysis of the results of microarray experiments paper we evaluate how well the system can retrieve functionally (Zhang et al., 2004; Al Shahrour et al., 2004).
    [Show full text]
  • 1 Meiotic Sex Chromosome Inactivation Is Disrupted in Sterile
    Genetics: Early Online, published on January 10, 2013 as 10.1534/genetics.112.148635 Meiotic sex chromosome inactivation is disrupted in sterile hybrid male house mice Polly Campbell*,1, Jeffrey M. Good§, and Michael W. Nachman* *Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721 §Division of Biological Sciences, University of Montana, Missoula, Montana 59812 1 Copyright 2013. Running head: Disrupted X inactivation in hybrid male mice Keywords: Haldane’s rule, meiosis, postmeiotic sex chromatin, speciation, spermatogenesis 1Corresponding author: Department of Ecology and Evolutionary Biology, Biosciences West 333, 1041 E Lowell St., University of Arizona, Tucson, AZ 85721. E-mail: [email protected] 2 ABSTRACT In male mammals, the X and Y chromosomes are transcriptionally silenced in primary spermatocytes by meiotic sex chromosome inactivation (MSCI) and remain repressed for the duration of spermatogenesis. Here, we test the longstanding hypothesis that disrupted MSCI might contribute to the preferential sterility of heterogametic hybrid males. We studied a cross between wild-derived inbred strains of Mus musculus musculus and M. m. domesticus in which sterility is asymmetric: F1 males with a M. m. musculus mother are sterile or nearly so while F1 males with a M. m. domesticus mother are normal. In previous work, we discovered widespread over-expression of X-linked genes in the testes of sterile but not fertile F1 males. Here, we ask whether this over-expression is specifically a result of disrupted MSCI. To do this, we isolated cells from different stages of spermatogenesis and measured the expression of several genes using quantitative PCR. We found that X over-expression in sterile F1 primary spermatocytes is coincident with the onset of MSCI and persists in postmeiotic spermatids.
    [Show full text]
  • Early-Life Exposure to the Antidepressant Fluoxetine Induces A
    Early-life exposure to the antidepressant fluoxetine induces a male-specific transgenerational disruption of the stress axis and exploratory behavior in adult zebrafish, Danio rerio Marilyn Nohely Vera Chang Thesis submitted to the Faculty of Graduate and Postdoctoral Studies in partial fulfillment of the requirements for the Doctorate in Philosophy degree in Biology with specialization in Chemical and Environmental Toxicology Department of Biology Faculty of Science University of Ottawa © Marilyn Nohely Vera Chang, Ottawa, Canada, 2018 ACKNOWLEDGMENTS Finishing this thesis has been both a challenging and gratifying experience. There are many people whom I must express my sincere gratitude toward as the work presented in this thesis would not have been possible without their contributions and never-ending support. First and foremost, I would like to acknowledge my supervisors, Dr. Vance Trudeau and Dr. Thomas Moon. I want to say how grateful I am for providing me with the opportunity to purse my academic and professional interests. I was first accepted by Tom and I will be forever thankful that he took a shot on me. Also, thanks to you Vance for adopting me when I was lab-less and for believing in my abilities as a scientist. Thank you to both of you for your outstanding guidance, patience and friendship which have given me an amazing experience in these past 6 years, you have been excellent mentors. Your concerns for your student’s well-being and mental health were bonus that I will not soon forget. I don’t think there are enough words to describe how thankful and fortunate I am to have benefit from your supervision not only at the academic and scientific level but also at the personal level, for all my accomplishments during this doctorate, I will forever be indebted to both of you.
    [Show full text]
  • Multiple Routes to Oncogenesis Are Promoted by the Human Papillomavirus–Host Protein Network
    Published OnlineFirst September 12, 2018; DOI: 10.1158/2159-8290.CD-17-1018 RESEARCH ARTICLE Multiple Routes to Oncogenesis Are Promoted by the Human Papillomavirus–Host Protein Network Manon Eckhardt 1 , 2 , 3 , Wei Zhang 4 , Andrew M. Gross 4 , John Von Dollen 1 , 3 , Jeffrey R. Johnson 1 , 2 , 3 , Kathleen E. Franks-Skiba1 , 3 , Danielle L. Swaney 1 , 2 , 3 , 5 , Tasha L. Johnson 3 , Gwendolyn M. Jang 1 , 3 , Priya S. Shah1 , 3 , Toni M. Brand 6 , Jacques Archambault 7 , Jason F. Kreisberg 4 , 5 , Jennifer R. Grandis 5 , 6 , Trey Ideker4 , 5 , and Nevan J. Krogan 1 , 2 , 3 , 5 ABSTRACT We have mapped a global network of virus–host protein interactions by purifi cation of the complete set of human papillomavirus (HPV) proteins in multiple cell lines followed by mass spectrometry analysis. Integration of this map with tumor genome atlases shows that the virus targets human proteins frequently mutated in HPV − but not HPV + cancers, providing a unique opportunity to identify novel oncogenic events phenocopied by HPV infection. For example, we fi nd that the NRF2 transcriptional pathway, which protects against oxidative stress, is activated by interaction of the NRF2 regulator KEAP1 with the viral protein E1. We also demonstrate that the L2 HPV protein physically interacts with the RNF20/40 histone ubiquitination complex and promotes tumor cell inva- sion in an RNF20/40-dependent manner. This combined proteomic and genetic approach provides a systematic means to study the cellular mechanisms hijacked by virally induced cancers. SIGNIFICANCE : In this study, we created a protein–protein interaction network between HPV and human proteins.
    [Show full text]
  • Histone Variants and Their Post-Translational Modifications in Primary Human Fat Cells
    Linköping University Post Print Histone Variants and Their Post-Translational Modifications in Primary Human Fat Cells Asa Jufvas, Peter Strålfors and Alexander Vener N.B.: When citing this work, cite the original article. Original Publication: Asa Jufvas, Peter Strålfors and Alexander Vener, Histone Variants and Their Post- Translational Modifications in Primary Human Fat Cells, 2011, PLOS ONE, (6), 1. http://dx.doi.org/10.1371/journal.pone.0015960 Licensee: Public Library of Science (PLoS) http://www.plos.org/ Postprint available at: Linköping University Electronic Press http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-65933 Histone Variants and Their Post-Translational Modifications in Primary Human Fat Cells A˚ sa Jufvas, Peter Stra˚lfors, Alexander V. Vener* Department of Clinical and Experimental Medicine, Linko¨ping University, Linko¨ping, Sweden Abstract Epigenetic changes related to human disease cannot be fully addressed by studies of cells from cultures or from other mammals. We isolated human fat cells from subcutaneous abdominal fat tissue of female subjects and extracted histones from either purified nuclei or intact cells. Direct acid extraction of whole adipocytes was more efficient, yielding about 100 mg of protein with histone content of 60% –70% from 10 mL of fat cells. Differential proteolysis of the protein extracts by trypsin or ArgC-protease followed by nanoLC/MS/MS with alternating CID/ETD peptide sequencing identified 19 histone variants. Four variants were found at the protein level for the first time; particularly HIST2H4B was identified besides the only H4 isoform earlier known to be expressed in humans. Three of the found H2A potentially organize small nucleosomes in transcriptionally active chromatin, while two H2AFY variants inactivate X chromosome in female cells.
    [Show full text]
  • A Multiprotein Occupancy Map of the Mrnp on the 3 End of Histone
    Downloaded from rnajournal.cshlp.org on October 6, 2021 - Published by Cold Spring Harbor Laboratory Press A multiprotein occupancy map of the mRNP on the 3′ end of histone mRNAs LIONEL BROOKS III,1 SHAWN M. LYONS,2 J. MATTHEW MAHONEY,1 JOSHUA D. WELCH,3 ZHONGLE LIU,1 WILLIAM F. MARZLUFF,2 and MICHAEL L. WHITFIELD1 1Department of Genetics, Dartmouth Geisel School of Medicine, Hanover, New Hampshire 03755, USA 2Integrative Program for Biological and Genome Sciences, University of North Carolina, Chapel Hill, North Carolina 27599, USA 3Department of Computer Science, University of North Carolina, Chapel Hill, North Carolina 27599, USA ABSTRACT The animal replication-dependent (RD) histone mRNAs are coordinately regulated with chromosome replication. The RD-histone mRNAs are the only known cellular mRNAs that are not polyadenylated. Instead, the mature transcripts end in a conserved stem– loop (SL) structure. This SL structure interacts with the stem–loop binding protein (SLBP), which is involved in all aspects of RD- histone mRNA metabolism. We used several genomic methods, including high-throughput sequencing of cross-linked immunoprecipitate (HITS-CLIP) to analyze the RNA-binding landscape of SLBP. SLBP was not bound to any RNAs other than histone mRNAs. We performed bioinformatic analyses of the HITS-CLIP data that included (i) clustering genes by sequencing read coverage using CVCA, (ii) mapping the bound RNA fragment termini, and (iii) mapping cross-linking induced mutation sites (CIMS) using CLIP-PyL software. These analyses allowed us to identify specific sites of molecular contact between SLBP and its RD-histone mRNA ligands. We performed in vitro crosslinking assays to refine the CIMS mapping and found that uracils one and three in the loop of the histone mRNA SL preferentially crosslink to SLBP, whereas uracil two in the loop preferentially crosslinks to a separate component, likely the 3′hExo.
    [Show full text]
  • Reduction in Reproductive Lifespan of Tissue Inhibitor of Metalloproteinase
    REPRODUCTIONRESEARCH RNA polymerase II interacts with the Hspa1b promoter in mouse epididymal spermatozoa Donald C Wilkerson and Kevin D Sarge Department of Molecular and Cellular Biochemistry, University of Kentucky, 741 S. Limestone Street, Lexington, Kentucky 40536, USA Correspondence should be addressed to K D Sarge; Email: [email protected] Abstract The Hspa1b (Hsp70.1) gene is one of the first genes expressed after fertilization, with expression occurring during the minor zygotic genome activation (ZGA) in the absence of stress. This expression can take place in the male pronucleus as early as the one-cell stage of embryogenesis. The importance of HSPA1B for embryonic viability during times of stress is supported by studies showing that depletion of this protein results in a significant reduction in embryos developing to the blastocyte stage. Recently, we have begun addressing the mechanism responsible for allowing expression of Hspa1b during the minor ZGA and found that heat shock transcription factor (HSF) 1 and 2 bind the Hspa1b promoter during late spermatogenesis. In this report, we have extended those studies using western blots and chromatin immunoprecipitation assays and found that RNA polymerase II (Pol II) is present in epididymal spermatozoa and bound to the Hspa1b promoter. These present results, in addition to our previous results, support a model in which the binding of HSF1, HSF2, SP1, and Pol II to the promoter of Hspa1b would allow the rapid formation of a transcription-competent state during the minor ZGA, thereby allowing Hspa1b expression. Reproduction (2009) 137 923–929 Introduction promoter-driven reporter genes confirm this embryonic pattern of expression (Thompson et al.
    [Show full text]
  • Ernas and Superenhancer Lncrnas Are Functional in Human Prostate Cancer
    Hindawi Disease Markers Volume 2020, Article ID 8847986, 17 pages https://doi.org/10.1155/2020/8847986 Research Article eRNAs and Superenhancer lncRNAs Are Functional in Human Prostate Cancer Xiaona Zhang,1,2,3 Panpan Pang,2,3,4 Min Jiang,1,2,3 Qunfa Cao,2,3 Huili Li,2,3 Yi Xu,5 Yao Li ,4 Xue Chen ,1 and Junsong Han 2,3 1Department of Pathology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China 2National Engineering Center for Biochip at Shanghai, Shanghai, China 3Shanghai Biochip Corporation, Shanghai, China 4Shanghai Engineering Research Center of Industrial Microorganisms, School of Life Science, Fudan University, Shanghai, China 5Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Chinese Medicine, Shanghai, China Correspondence should be addressed to Yao Li; [email protected], Xue Chen; [email protected], and Junsong Han; [email protected] Received 23 June 2020; Revised 27 July 2020; Accepted 14 August 2020; Published 24 September 2020 Academic Editor: Mingjun Shi Copyright © 2020 Xiaona Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Prostate cancer (PCa) is one of the most commonly diagnosed cancers in males worldwide. lncRNAs (long noncoding RNAs) play a significant role in the occurrence and development of PCa. eRNAs (enhancer RNAs) and SE-lncRNAs (superenhancer lncRNAs) are important elements of lncRNAs, but the role of eRNAs and SE-lncRNAs in PCa remains largely unclear. In this work, we identified 681 eRNAs and 292 SE-lncRNAs that were expressed differentially in PCa using a microarray.
    [Show full text]
  • Supplemental Data.Pdf
    Supplementary material -Table of content Supplementary Figures (Fig 1- Fig 6) Supplementary Tables (1-13) Lists of genes belonging to distinct biological processes identified by GREAT analyses to be significantly enriched with UBTF1/2-bound genes Supplementary Table 14 List of the common UBTF1/2 bound genes within +/- 2kb of their TSSs in NIH3T3 and HMECs. Supplementary Table 15 List of gene identified by microarray expression analysis to be differentially regulated following UBTF1/2 knockdown by siRNA Supplementary Table 16 List of UBTF1/2 binding regions overlapping with histone genes in NIH3T3 cells Supplementary Table 17 List of UBTF1/2 binding regions overlapping with histone genes in HMEC Supplementary Table 18 Sequences of short interfering RNA oligonucleotides Supplementary Table 19 qPCR primer sequences for qChIP experiments Supplementary Table 20 qPCR primer sequences for reverse transcription-qPCR Supplementary Table 21 Sequences of primers used in CHART-PCR Supplementary Methods Supplementary Fig 1. (A) ChIP-seq analysis of UBTF1/2 and Pol I (POLR1A) binding across mouse rDNA. UBTF1/2 is enriched at the enhancer and promoter regions and along the entire transcribed portions of rDNA with little if any enrichment in the intergenic spacer (IGS), which separates the rDNA repeats. This enrichment coincides with the distribution of the largest subunit of Pol I (POLR1A) across the rDNA. All sequencing reads were mapped to the published complete sequence of the mouse rDNA repeat (Gene bank accession number: BK000964). The graph represents the frequency of ribosomal sequences enriched in UBTF1/2 and Pol I-ChIPed DNA expressed as fold change over those of input genomic DNA.
    [Show full text]
  • CREB-Dependent Transcription in Astrocytes: Signalling Pathways, Gene Profiles and Neuroprotective Role in Brain Injury
    CREB-dependent transcription in astrocytes: signalling pathways, gene profiles and neuroprotective role in brain injury. Tesis doctoral Luis Pardo Fernández Bellaterra, Septiembre 2015 Instituto de Neurociencias Departamento de Bioquímica i Biologia Molecular Unidad de Bioquímica y Biologia Molecular Facultad de Medicina CREB-dependent transcription in astrocytes: signalling pathways, gene profiles and neuroprotective role in brain injury. Memoria del trabajo experimental para optar al grado de doctor, correspondiente al Programa de Doctorado en Neurociencias del Instituto de Neurociencias de la Universidad Autónoma de Barcelona, llevado a cabo por Luis Pardo Fernández bajo la dirección de la Dra. Elena Galea Rodríguez de Velasco y la Dra. Roser Masgrau Juanola, en el Instituto de Neurociencias de la Universidad Autónoma de Barcelona. Doctorando Directoras de tesis Luis Pardo Fernández Dra. Elena Galea Dra. Roser Masgrau In memoriam María Dolores Álvarez Durán Abuela, eres la culpable de que haya decidido recorrer el camino de la ciencia. Que estas líneas ayuden a conservar tu recuerdo. A mis padres y hermanos, A Meri INDEX I Summary 1 II Introduction 3 1 Astrocytes: physiology and pathology 5 1.1 Anatomical organization 6 1.2 Origins and heterogeneity 6 1.3 Astrocyte functions 8 1.3.1 Developmental functions 8 1.3.2 Neurovascular functions 9 1.3.3 Metabolic support 11 1.3.4 Homeostatic functions 13 1.3.5 Antioxidant functions 15 1.3.6 Signalling functions 15 1.4 Astrocytes in brain pathology 20 1.5 Reactive astrogliosis 22 2 The transcription
    [Show full text]
  • Transcriptional Regulators Are Upregulated in the Substantia Nigra
    Journal of Emerging Investigators Transcriptional Regulators are Upregulated in the Substantia Nigra of Parkinson’s Disease Patients Marianne Cowherd1 and Inhan Lee2 1Community High School, Ann Arbor, MI 2miRcore, Ann Arbor, MI Summary neurological conditions is an established practice (3). Parkinson’s disease (PD) affects approximately 10 Significant gene expression dysregulation in the SN and million people worldwide with tremors, bradykinesia, in the striatum has been described, particularly decreased apathy, memory loss, and language issues. Though such expression in PD synapses. Protein degradation has symptoms are due to the loss of the substantia nigra (SN) been found to be upregulated (4). Mutations in SNCA brain region, the ultimate causes and complete pathology are unknown. To understand the global gene expression (5), LRRK2 (6), and GBA (6) have also been identified changes in SN, microarray expression data from the SN as familial markers of PD. SNCA encodes alpha- tissue of 9 controls and 16 PD patients were compared, synuclein, a protein found in presynaptic terminals that and significantly upregulated and downregulated may regulate vesicle presence and dopamine release. genes were identified. Among the upregulated genes, Eighteen SNCA mutations have been associated with a network of 33 interacting genes centered around the PD and, although the exact pathogenic mechanism is cAMP-response element binding protein (CREBBP) was not confirmed, mutated alpha-synuclein is the major found. The downstream effects of increased CREBBP- component of protein aggregates, called Lewy bodies, related transcription and the resulting protein levels that are often found in PD brains and may contribute may result in PD symptoms, making CREBBP a potential therapeutic target due to its central role in the interactive to cell death.
    [Show full text]
  • Network-Based Analysis of Key Regulatory Genes Implicated in Type
    www.nature.com/scientificreports OPEN Network‑based analysis of key regulatory genes implicated in Type 2 Diabetes Mellitus and Recurrent Miscarriages in Turner Syndrome Anam Farooqui1, Alaa Alhazmi2, Shaful Haque3, Naaila Tamkeen4, Mahboubeh Mehmankhah1, Safa Tazyeen1, Sher Ali5 & Romana Ishrat1* The information on the genotype–phenotype relationship in Turner Syndrome (TS) is inadequate because very few specifc candidate genes are linked to its clinical features. We used the microarray data of TS to identify the key regulatory genes implicated with TS through a network approach. The causative factors of two common co‑morbidities, Type 2 Diabetes Mellitus (T2DM) and Recurrent Miscarriages (RM), in the Turner population, are expected to be diferent from that of the general population. Through microarray analysis, we identifed nine signature genes of T2DM and three signature genes of RM in TS. The power‑law distribution analysis showed that the TS network carries scale‑free hierarchical fractal attributes. Through local‑community‑paradigm (LCP) estimation we fnd that a strong LCP is also maintained which means that networks are dynamic and heterogeneous. We identifed nine key regulators which serve as the backbone of the TS network. Furthermore, we recognized eight interologs functional in seven diferent organisms from lower to higher levels. Overall, these results ofer few key regulators and essential genes that we envisage have potential as therapeutic targets for the TS in the future and the animal models studied here may prove useful in the validation of such targets. Te medical systems and scientists throughout the world are under an unprecedented challenge to meet the medical needs of much of the world’s population that are sufering from chromosomal anomalies.
    [Show full text]