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Understanding the Pathogenesis of Porcine Circovirus Type 2 (PCV2)-Associated Diseases Tanja Ilse Opriessnig Iowa State University

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Understanding the Pathogenesis of Porcine Circovirus Type 2 (PCV2)-Associated Diseases Tanja Ilse Opriessnig Iowa State University Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 2006 Understanding the pathogenesis of porcine circovirus type 2 (PCV2)-associated diseases Tanja Ilse Opriessnig Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Microbiology Commons, and the Veterinary Pathology and Pathobiology Commons Recommended Citation Opriessnig, Tanja Ilse, "Understanding the pathogenesis of porcine circovirus type 2 (PCV2)-associated diseases " (2006). Retrospective Theses and Dissertations. 1481. https://lib.dr.iastate.edu/rtd/1481 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Understanding the pathogenesis of porcine circovirus type 2 (PCV2)-associated diseases by Tanja Ilse Opriessnig A dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Major: Veterinary Pathology Program of Study Committee: Patrick G. Halbur, Major Professor Bruce H. Janke Mark R. Ackermann Richard B. Evans Eileen L. Thacker Iowa State University Ames, Iowa 2006 Copyright © Tanja Ilse Opriessnig, 2006. All rights reserved. UMI Number: 3218983 UMI Microform 3218983 Copyright 2006 by ProQuest Information and Learning Company. All rights reserved. This microform edition is protected against unauthorized copying under Title 17, United States Code. ProQuest Information and Learning Company 300 North Zeeb Road P.O. Box 1346 Ann Arbor, MI 48106-1346 ii Graduate College Iowa State University NOTE: Electronic theses will not contain the signed thesis approval page here. iii TABLE OF CONTENTS ABSTRACT ..........................................................................................................................viii CHAPTER 1. GENERAL INTRODUCTION ........................................................................ 1 Disseration Organization .......................................................................................................... 1 Introduction............................................................................................................................... 2 CHAPTER 2. LITERATURE REVIEW ................................................................................. 6 Historical Background .............................................................................................................. 6 Taxonomy ................................................................................................................................. 7 Biologic and Physical Properties .............................................................................................. 8 Replication and Binding Strategy ............................................................................................. 8 Genome Organization, Genetic Characterization, and Proteins ............................................. 10 Host Range.............................................................................................................................. 10 Epidemiology.......................................................................................................................... 15 Pathogenesis............................................................................................................................ 18 PCV2-associated Diseases...................................................................................................... 23 Factors that play a Role in PCV2-associated Diseases........................................................... 35 Diagnosis................................................................................................................................. 44 Control of PCV2-associated Diseases .................................................................................... 50 CHAPTER 3. DERIVATION OF PORCINE CIRCOVIRUS TYPE 2-NEGATIVE PIGS FROM POSITIVE BREEDING HERDS............................................................................. 57 Summary................................................................................................................................. 57 Introduction............................................................................................................................. 58 Materials and Methods............................................................................................................ 58 Results .................................................................................................................................... 61 Discussion............................................................................................................................... 63 Implications............................................................................................................................. 65 iv References............................................................................................................................... 66 CHAPTER 4. EFFECT OF VACCINATION WITH SELECTIVE BACTERINS ON CONVENTIONAL PIGS INFECTED WITH TYPE 2 PORCINE CIRCOVIRUS............ 76 Abstract................................................................................................................................... 76 Introduction............................................................................................................................. 77 Materials and Methods............................................................................................................ 78 Results..................................................................................................................................... 82 Discussion............................................................................................................................... 85 Acknowledgments................................................................................................................... 88 References............................................................................................................................... 88 CHAPTER 5. EFFECTS OF THE TIMING OF THE ADMINISTRATION OF MYCOPLASMA HYOPNEUMONIAE BACTERIN ON THE DEVELOPMENT OF LESIONS ASSOCIATED WITH PORCINE CIRCOVIRUS TYPE 2............................... 99 Abstract................................................................................................................................... 99 Introduction........................................................................................................................... 100 Materials and Methods.......................................................................................................... 100 Results................................................................................................................................... 104 Discussion............................................................................................................................. 106 Acknowledgments................................................................................................................. 110 References............................................................................................................................. 110 CHAPTER 6. PORCINE CIRCOVIRUS TYPE 2 INFECTION DECREASES THE EFFICACY OF A MODIFIED LIVE PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS VACCINE.......................................................... 123 Abstract................................................................................................................................. 123 Introduction........................................................................................................................... 124 Materials and Methods.......................................................................................................... 126 Results................................................................................................................................... 130 v Discussion............................................................................................................................. 132 Acknowledgments................................................................................................................. 135 References............................................................................................................................. 135 CHAPTER 7. EFFECT OF PORCINE PARVOVIRUS VACCINATION ON THE DEVELOPMENT OF PMWS IN SEGREGATED EARLY WEANED PIGS COINFECTED WITH TYPE 2 PORCINE CIRCOVIRUS AND PORCINE PARVOVIRUS................................................................................................................... 146 Abstract................................................................................................................................. 146 Introduction........................................................................................................................... 147 Materials and Methods.......................................................................................................... 149 Results..................................................................................................................................
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