Sensing How to Balance
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Ultrastructural Study of the Granule Cell Domain of the Cochlear Nucleus in Rats: Mossy Fiber Endings and Their Targets
THE JOURNAL OF COMPARATIVE NEUROLOGY 369~345-360 ( 1996) Ultrastructural Study of the Granule Cell Domain of the Cochlear Nucleus in Rats: Mossy Fiber Endings and Their Targets DIANA L. WEEDMAN, TAN PONGSTAPORN, AND DAVID K. RYUGO Center for Hearing Sciences, Departments of Otolaryngoloby-Head and Neck Surgery and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 2 1205 ABSTRACT The principal projection neurons of the cochlear nucleus receive the bulk of their input from the auditory nerve. These projection neurons reside in the core of the nucleus and are surrounded by an external shell, which is called the granule cell domain. Interneurons of the cochlear granule cell domain are the target for nonprimary auditory inputs, including projections from the superior olivary complex, inferior colliculus, and auditory cortex. The granule cell domain also receives projections from the cuneate and trigeminal nuclei, which are first-order nuclei of the somatosensory system. The cellular targets of the nonprimary projections are mostly unknown due to a lack of information regarding postsynaptic profiles in the granule cell areas. In the present paper, we examined the synaptic relationships between a heterogeneous class of large synaptic terminals called mossy fibers and their targets within subdivisions of the granule cell domain known as the lamina and superficial layer. By using light and electron microscopic methods in these subdivisions, we provide evidence for three different neuron classes that receive input from the mossy fibers: granule cells, unipolar brush cells, and a previously undescribed class called chestnut cells. The distinct synaptic relations between mossy fibers and members of each neuron class further imply fundamentally separate roles for processing acoustic signals. -
Consensus Paper: Cerebellar Development
Cerebellum DOI 10.1007/s12311-015-0724-2 CONSENSUS PAPER Consensus Paper: Cerebellar Development Ketty Leto1,2 & Marife Arancillo3 & Esther B. E. Becker4 & Annalisa Buffo1,2 & Chin Chiang5 & Baojin Ding6 & William B. Dobyns 7,8 & Isabelle Dusart9,10 & Parthiv Haldipur7 & Mary E. Hatten11 & Mikio Hoshino12 & Alexandra L. Joyner13 & Masanobu Kano14 & Daniel L. Kilpatrick6 & Noriyuki Koibuchi15 & Silvia Marino16 & Salvador Martinez17 & Kathleen J. Millen7 & Thomas O. Millner16 & Takaki Miyata18 & Elena Parmigiani1,2 & Karl Schilling19 & Gabriella Sekerková20 & Roy V. Sillitoe3 & Constantino Sotelo21 & Naofumi Uesaka14 & Annika Wefers 22 & Richard J. T. Wingate23 & Richard Hawkes24 # The Author(s) 2015. This article is published with open access at Springerlink.com Abstract The development of the mammalian cerebellum is processes of cerebellar ontogenesis, highlighting the neuro- orchestrated by both cell-autonomous programs and inductive genic strategies used by developing progenitors, the genetic environmental influences. Here, we describe the main programs involved in cell fate specification, the progressive * Ketty Leto 10 Centre National de la Recherche Scientifique, CNRS, UMR8246, [email protected] INSERM U1130, Neuroscience Paris Seine, France, 75005 Paris, France 11 Laboratory of Developmental Neurobiology, The Rockefeller 1 Department of Neuroscience Rita Levi Montalcini, University of University, New York, NY 10065, USA Turin, via Cherasco 15, 10026 Turin, Italy 12 Department of Biochemistry and Cellular Biology, National Institute -
Purkinje Cell Migration Disorder By
CEREBELLAR CORTICOGENESIS IN THE LYSOSOMAL ACID PHOSPHATASE (ACP2) MUTANT MICE: PURKINJE CELL MIGRATION DISORDER BY NILOUFAR ASHTARI A Thesis Submitted to the Faculty of Graduate Studies of The University of Manitoba in Partial Fulfilment of the Requirements for the Degree of MASTER OF SCIENCE Department of Human Anatomy and Cell Science University of Manitoba Winnipeg, Manitoba Copyright © 2017 by Niloufar Ashtari 1 Abstract In a mutant mouse called nax as the result of mutation in Lysosomal Acid phosphatase (Acp2), layers of the cerebellar cortex are impaired and monolayer Purkinje cells (Pcs) turn to multi-layered Pcs that ectopically invade the molecular layer. We investigated reelin-Dab1 signaling as an important pathway for Pcs migration and monolayer formation in cerebellum. ERK1/2 is a member of mitogen activated kinases family and suggested to be a downstream of reelin signaling. We hypothesize that the establishment of mono-layered Pcs rely on reelin through ERK1/2 pathway. Acp2 mutant mice were used for this study and molecular expression and distribution were assessed by immunohistochemistry, RT-PCR, western blotting, and cell culture. Results suggest that reelin may modulate the ERK1/2 expression, thus lower expression of reelin and higher phosphorylation of Dab1 leads to over expression of the ERK1/2 that causes the Pcs to over migrate and form multilayer in nax cerebellar cortex. i TABLE OF CONTENTS LISTOFABBREVIATIONS………………………………………………..…... IV LIST OF TABLES……………………………………...…………………...….. Vii LIST OF FIGURES…………………………………………………….………. Viii CHAPTER 1: INTRODUCTION…………………………………….………… 1 1.1 Cerebellum ……………………………………………………........………. 1 1.2 Development of Central Nervous System………………………………….. 2 1.3 Development of the cerebellum………………………………….................. 3 1.4 Specification of cerebellar germinal zones…………………………………. -
Staining of Cerebellar Cortex Granular Layer Interneurons with Natural Dye of Madder Anneh Mohammad Gharravi
Gharravi Cerebellum & Ataxias (2016) 3:12 DOI 10.1186/s40673-016-0050-6 RESEARCH Open Access Staining of cerebellar cortex granular layer interneurons with natural dye of Madder Anneh Mohammad Gharravi Abstract Background: The objective of the present study was an investigation of root Rubia Tinctorum (Madder) as a natural dye to identification of granular layer interneurons of the rat cerebellum. Methods: Seven to ten micrometre sections were collected from the cerebellum and stained only with Madder for 2, 24 and 48 h. Other sections were stained with Madder then with hematoxyllin, cresyl violet, eosin, light green. Microscopic identification of cells was performed based on cell morphology, reaction and binding of with the dye. All data were expressed as mean ± SD in and significance was set at p ≤0.05. Results: Madder with alum as mordant resulted a deep red staining of interneurons. Unipolar brush cells (UBCs) were observed with a cell body diameter intermediate between granule and Golgi cells in the superficial layer of the granular layer. Golgi cells were identified almost as large as Purkinje cells with irregular rounded or polygonal morphology. Lugaro cells were observed as spindle-shaped cells adjacent to Purkinje layer. Conclusion: Results of the present study showed that mader could stain granular layer interneurons in cerebellum cortex of rat. Keywords: Madder, Cerebellum, Unipolar brush cells, Lugaro cell, Golgi neurons Background with all cerebellar cortical neurons and fibers and they Histologically, the cerebellar cortex is divided into three function as inhibitory interneurons. These spindle-shaped layers: the molecular, the Purkinje and the granular cells locate just underneath the Purkinje cell layer are layers. -
Use of Calcium-Binding Proteins to Map Inputs in Vestibular Nuclei of the Gerbil
THE JOURNAL OF COMPARATIVE NEUROLOGY 386:317–327 (1997) Use of Calcium-Binding Proteins to Map Inputs in Vestibular Nuclei of the Gerbil GOLDA ANNE KEVETTER* AND ROBERT B. LEONARD Departments of Otolaryngology, Anatomy and Neurosciences, and Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas 77555 ABSTRACT We wished to determine whether calbindin and/or calretinin are appropriate markers for vestibular afferents, a population of neurons in the vestibular nuclear complex, or cerebellar Purkinje inputs. To accomplish this goal, immunocytochemical staining was observed in gerbils after lesions of the vestibular nerve central to the ganglion, the cerebellum, or both. Eleven to fourteen days after recovery, the brain was processed for immunocytochemical identification of calretinin and calbindin. After lesion of the vestibular nerve, no calretinin staining was seen in any of the vestibular nuclei except for a population of intrinsic neurons, which showed no obvious change in number or staining pattern. Calbindin staining was reduced in all nuclei except the dorsal part of the lateral vestibular nuclei. The density of staining of each marker, measured in the magnocellular medial vestibular nucleus, was signifi- cantly reduced. After the cerebellar lesion, no differences in calretinin staining were noted. However, calbindin staining was greatly reduced in all nuclei. The density of staining, measured in the caudal medial vestibular nucleus, was significantly lower. After a combined lesion of the cerebellum and vestibular nerve, the distribution and density of calretinin staining resembled that after vestibular nerve section alone, whereas calbindin staining was no longer seen. This study demonstrates that calretinin and calbindin are effective markers for the identification of vestibular afferents. -
Cranial Nerves 1, 5, 7-12
Cranial Nerve I Olfactory Nerve Nerve fiber modality: Special sensory afferent Cranial Nerves 1, 5, 7-12 Function: Olfaction Remarkable features: – Peripheral processes act as sensory receptors (the other special sensory nerves have separate Warren L Felton III, MD receptors) Professor and Associate Chair of Clinical – Primary afferent neurons undergo continuous Activities, Department of Neurology replacement throughout life Associate Professor of Ophthalmology – Primary afferent neurons synapse with secondary neurons in the olfactory bulb without synapsing Chair, Division of Neuro-Ophthalmology first in the thalamus (as do all other sensory VCU School of Medicine neurons) – Pathways to cortical areas are entirely ipsilateral 1 2 Crania Nerve I Cranial Nerve I Clinical Testing Pathology Anosmia, hyposmia: loss of or impaired Frequently overlooked in neurologic olfaction examination – 1% of population, 50% of population >60 years Aromatic stimulus placed under each – Note: patients with bilateral anosmia often report nostril with the other nostril occluded, eg impaired taste (ageusia, hypogeusia), though coffee, cloves, or soap taste is normal when tested Note that noxious stimuli such as Dysosmia: disordered olfaction ammonia are not used due to concomitant – Parosmia: distorted olfaction stimulation of CN V – Olfactory hallucination: presence of perceived odor in the absence of odor Quantitative clinical tests are available: • Aura preceding complex partial seizures of eg, University of Pennsylvania Smell temporal lobe origin -
Cranial Nerve VIII
Cranial Nerve VIII Color Code Important (The Vestibulo-Cochlear Nerve) Doctors Notes Notes/Extra explanation Please view our Editing File before studying this lecture to check for any changes. Objectives At the end of the lecture, the students should be able to: ✓ List the nuclei related to vestibular and cochlear nerves in the brain stem. ✓ Describe the type and site of each nucleus. ✓ Describe the vestibular pathways and its main connections. ✓ Describe the auditory pathway and its main connections. Due to the difference of arrangement of the lecture between the girls and boys slides we will stick to the girls slides then summarize the pathway according to the boys slides. Ponto-medullary Sulcus (cerebello- pontine angle) Recall: both cranial nerves 8 and 7 emerge from the ventral surface of the brainstem at the ponto- medullary sulcus (cerebello-pontine angle) Brain – Ventral Surface Vestibulo-Cochlear (VIII) 8th Cranial Nerve o Type: Special sensory (SSA) o Conveys impulses from inner ear to nervous system. o Components: • Vestibular part: conveys impulses associated with body posture ,balance and coordination of head & eye movements. • Cochlear part: conveys impulses associated with hearing. o Vestibular & cochlear parts leave the ventral surface* of brain stem through the pontomedullary sulcus ‘at cerebellopontine angle*’ (lateral to facial nerve), run laterally in posterior cranial fossa and enter the internal acoustic meatus along with 7th (facial) nerve. *see the previous slide Auditory Pathway Only on the girls’ slides 04:14 Characteristics: o It is a multisynaptic pathway o There are several locations between medulla and the thalamus where axons may synapse and not all the fibers behave in the same manner. -
Imaging Features Differentiating Vestibular Ganglion From
Click HERE for more articles at Annals, Academy of Medicine, Singapore homepage Differentiating Ganglion from Schwannoma—Yi-Wei Wu et al 65 Original Article Imaging Features Differentiating Vestibular Ganglion from Intracanalicular Schwannoma on Single-Sequence Non-Contrast Magnetic Resonance Imaging Study 1 1 1 2 Yi-Wei Wu, MD, MMed, FRCR, Amit Karandikar, MBBS, FRCR, FAMS, Julian PN Goh, MBBS, FRCR, Tiong Yong Tan, MBBS, FRCR, FAMS Abstract Introduction: This study aimed to identify imaging features on single-sequence non- contrast magnetic resonance imaging (MRI) that differentiate the vestibular ganglion from small intracanalicular schwannomas. Materials and Methods: Ninety patients (42 men and 48 women; age: 24‒87 years old) with 102 internal auditory canal (IAC) nodules (59 vestibular ganglia and 43 intracanalicular schwannoma) who underwent both single- sequence T2-weighted (T2W) non-contrast enhanced MRI studies and contrast-enhanced T1-weighted (T1W) MRI studies between May 2012 and April 2017 were evaluated. The length, width, distance to the IAC fundus and length/width ratios for all lesions were obtained and compared among groups. Diagnostic performance and cutoff values of the parameters were evaluated with receiver operating characteristics curve analysis. Area under the curve (AUC) value was calculated. Results: Vestibular ganglia have significantly smaller lengths and widths compared to intracanalicular vestibular schwannomas (1.7 ± 0.4 mm and 1.0 ± 0.2 mm versus 5.6 ± 3.0 mm and 3.7 ± 1.5 mm). They are more fusiform in shape compared to vestibular schwannomas (length/width ratio: 1.8 ± 0.4 versus 1.5 ± 0.4). The lesion width demonstrated the highest diagnostic performance (AUC: 0.998). -
M. Dauzvardis, Phd. 7/10/12 NERVES LISTED ACCORDING to FUNCTIONAL COMPONENTS
M. Dauzvardis, PhD. 7/10/12 NERVES LISTED ACCORDING TO FUNCTIONAL COMPONENTS SPECIAL SOMATIC AFFERENT (SSA) II. Optic: Optic stimuli are received by the rods and cones, relayed to second and third order neurons which comprise the optic nerve. (About half the fibers decussate in the optic chiasma). Impulses are along the optic nerve and optic tract to the lateral geniculate bodies of the diecephalon. Relay is then from the lateral geniculate bodies to the calcarine fissure area of the occipital lobes. VIII. Auditory (Acoustic) (Vestibulocochlear) A. Sound stimulates hair cells (receptors) of the Organ of Corti in the cochlea and impulses are carried along bipolar neurons with their cell bodies in the spiral ganglion to the dorsal and ventral cochlear nuclei of the medulla (hearing). B. Motion of the endolymph stimulates receptors in the sacculus and utriculus and the ampullae of the three semicircular canals. These impulses are carried along bipolar neurons whose cell bodies are in the vestibular ganglion and terminate in the medial, lateral, superior and spinal vestibular nuclei of the medulla (equilibrium). GENERAL SOMATIC AFFERENT (GSA) V. Trigeminal Ophthalmic branch: from cornea, conjunctiva, eyelid, forehead and nose. Maxillary branch: from skin of cheek, lower lid, side of nose and upper jaw, upper teeth, mucosa of mouth and maxillary sinuses. Mandibular branch: from skin of ear pinna, ear canal, temporal area, lower jaw and teeth, mucosa of mouth, gums and general sensation from anterior two‐thirds of the tongue. All of the above neurons have their cell bodies in the trigeminal ganglion and terminate in the sensory nuclei of the 5th nerve. -
Forward Signaling by Unipolar Brush Cells in the Mouse Cerebellum
Cerebellum DOI 10.1007/s12311-015-0693-5 ORIGINAL PAPER Forward Signaling by Unipolar Brush Cells in the Mouse Cerebellum Stijn van Dorp1 & Chris I. De Zeeuw 1,2 # The Author(s) 2015. This article is published with open access at Springerlink.com Abstract Unipolar brush cells (UBCs) are glutamatergic in- Introduction terneurons prominently present in the granular layer of the vestibulocerebellum. UBCs engage in extensive synaptic con- Forward processing, in particular the absence of recurrent tact with a single presynaptic mossy fiber and signal to down- excitation, is a defining feature of cerebellar architecture stream granule cells through an elaborate network of mossy and computation. In the granular layer of the fiber-like axons. Ultrastructural examinations and electro- vestibulocerebellum, unipolar brush cells (UBCs) provide physiological recordings in organotypic slice cultures have a powerful forward excitatory action onto granule cells indicated that UBCs target not only granule cells but also other through a cortex-intrinsic network of mossy fiber-like UBCs, thus forming chains of two or perhaps more intercon- axons [1, 2]. UBCs are characterized by an elaborate nected UBCs. In this report, we show recordings of spontane- brush-like dendrite (Fig. 1a) that forms an unusually exten- ous and evoked (di)synaptic events in granule cells and UBCs sive synaptic contact with a single presynaptic mossy fiber in fresh cerebellar slices from juvenile mice (5–7 weeks). The rosette [3]. This highly specialized configuration has been patterns of arrival of synaptic events were consistent with the proposed to facilitate prolonged entrapment of glutamate presence of a presynaptic UBC, and recordings from UBCs in the synaptic cleft, underlying complex temporal trans- displayed spontaneous protracted synaptic events characteris- formations of incoming mossy fiber signals [4, 5]. -
Auditory & Vestibular Systems Steven Mcloon Department of Neuroscience University of Minnesota
Auditory & Vestibular Systems Steven McLoon Department of Neuroscience University of Minnesota 1 The auditory & vestibular systems have many similarities. • The sensory apparatus for both are in canals embedded in the bone of the inner ear. • Receptor cells (hair cells) for both are mechanosensory cells with fine stereocilia. • Information for both is carried into the brain via the vestibulochoclear nerve (cranial nerve VIII). 2 Auditory System • The auditory system detects and interprets sound. • Sound is the vibration of air molecules similar to ripples in water that propagate from a thrown rock. • The sound waves have an amplitude (loudness) and frequency (pitch). 3 Auditory System • Humans can typically hear 20 – 20,000 hertz (cycles per second). 4 Auditory System • Humans can typically hear 20 – 20,000 hertz (cycles per second). http://en.wikipedia.org/wiki/Audio_frequency 5 Auditory System • As a person ages, he/she loses the ability to hear high and low frequencies. 6 Auditory System External ear: • includes the pinna, external auditory meatus (ear canal) and tympanic membrane (ear drum). • The pinna and canal collect sound and guide it to the tympanic membrane. • The tympanic membrane vibrates in response to sound. 7 Auditory System Middle ear: • It is an air filled chamber. • The eustachian tube (auditory tube) connects the middle ear chamber with the pharynx (throat). • Three tiny bones in the chamber transfer the vibration of the tympanic membrane to the oval window of the inner ear. • Two tiny muscles can dampen the movement of the tympanic membrane and bones to protect against a loud sound. 8 Auditory System Inner ear: • The cochlea is a snail shaped tube incased in bone. -
Early Trigeminal Ganglion Afferents Enter the Cerebellum Before the Purkinje Cells Are Born and Target the Nuclear Transitory Zo
Brain Structure and Function (2019) 224:2421–2436 https://doi.org/10.1007/s00429-019-01916-7 ORIGINAL ARTICLE Early trigeminal ganglion aferents enter the cerebellum before the Purkinje cells are born and target the nuclear transitory zone Hassan Marzban1,3 · Maryam Rahimi‑Balaei1 · Richard Hawkes2 Received: 24 May 2018 / Accepted: 25 June 2019 / Published online: 29 June 2019 © Springer-Verlag GmbH Germany, part of Springer Nature 2019 Abstract In the standard model for the development of climbing and mossy fber aferent pathways to the cerebellum, the ingrowing axons target the embryonic Purkinje cell somata (around embryonic ages (E13–E16 in mice). In this report, we describe a novel earlier stage in aferent development. Immunostaining for a neuroflament-associated antigen (NAA) reveals the early axon distributions with remarkable clarity. Using a combination of DiI axon tract tracing, analysis of neurogenin1 null mice, which do not develop trigeminal ganglia, and mouse embryos maintained in vitro, we show that the frst axons to innervate the cerebellar primordium as early as E9 arise from the trigeminal ganglion. Therefore, early trigeminal axons are in situ before the Purkinje cells are born. Double immunostaining for NAA and markers of the diferent domains in the cerebellar primordium reveal that aferents frst target the nuclear transitory zone (E9–E10), and only later (E10–E11) are the axons, either collaterals from the trigeminal ganglion or a new aferent source (e.g., vestibular ganglia), seen in the Purkinje cell plate. The fnding that the earliest axons to the cerebellum derive from the trigeminal ganglion and enter the cerebellar primordium before the Purkinje cells are born, where they seem to target the cerebellar nuclei, reveals a novel stage in the development of the cerebellar aferents.