(12) United States Patent (10) Patent No.: US 6,958,472 B2 Zubarev (45) Date of Patent: Oct
USOO6958472B2 (12) United States Patent (10) Patent No.: US 6,958,472 B2 Zubarev (45) Date of Patent: Oct. 25, 2005 (54) MASS SPECTROMETRY METHODS USING 4,988,869 A 1/1991 Aberth ELECTRON CAPTURE BY ONS 5,340,983 A 8/1994 Deinzer et al. 5,374,828 A 12/1994 Boumseek et al. (75) Inventor: Roman Zubarev, Uppsala (SE) 5,493,115 A * 2/1996 Deinzer et al. ............. 250/281 6,653,622 B2 * 11/2003 Franzen ............ ... 250/282 (73) Assignee: Syddansk Universitet, Odense (DK) 2003/0183760 A1 * 10/2003 Tsybin et al. ............... 250/292 (*) Notice: Subject to any disclaimer, the term of this OTHER PUBLICATIONS patent is extended or adjusted under 35 R. W. Vachet et al., “Novel Peptide Dissociation: Gas-Phase U.S.C. 154(b) by 0 days. Intramolecular Rearrangement of Internal Amino Acid Resi (21) Appl. No.: 10/471.454 dues”, Jun. 18, 1997, pp. 5481-5488, Journal of the Ameri can Chemical Society, vol. 119, No. 24. (22) PCT Filed: Mar. 22, 2002 (86) PCT No.: PCT/DK02/00195 (Continued) S371 (c)(1), Primary Examiner John R. Lee (2), (4) Date: Apr. 2, 2004 ASSistant Examiner Zia R. Hashmi (74) Attorney, Agent, or Firm-Harness, Dickey & Pierce, (87) PCT Pub. No.: WO02/078048 PLC PCT Pub. Date: Oct. 3, 2002 (57) ABSTRACT (65) Prior Publication Data Methods and apparatus are provided to obtain efficient US 2004/015.5180 A1 Aug. 12, 2004 Electron capture dissociation (ECD) of positive ions, par ticularly useful in the mass Spectrometric analysis of com Related U.S. Application Data plex Samples Such as of complex mixtures and large bio (60) Provisional application No.
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