14Th International Botrytis Symposium Abstract Book
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Three New Species of B Otryotinia on Ranunculaceae1
THREE NEW SPECIES OF B OTRYOTINIA ON RANUNCULACEAE1 G. L. HENNEBERT2 Institut agronomique, Universite de Louvain, Hervelee, Belgium AND J. W. GROVES3 Research Branch, Department of Agriculture, Ottawa, Canada Received October 10, 1962 Abstract Three new species of Botryotinia on Caltha palustris L., Ranunculus septen- trionalis Poir., and Ficaria verna Huds. (Ranunculaceae) are described as B. calthae Hennebert and Elliott, B. ranunculi Hennebert and Groves, and B. ficariarum Hennebert. Each of the three species has a Botrytis state of the 73. cinerea complex, and they thus constitute additions to the species already segregated from that complex, i.e. Botryotinia fuckeliana, 73. convoluta, B. draytoni, and B. pelargonii. The Botrytis state of B. ficariarum can be distinguished morp hologically. While B. ranunculi is a North American species and B. ficariarum an European one, B. calthae is reported from both continents. Introduction Botryotinia, a genus of the Sclerotiniaceae, was erected by Whetzel (1945) for four species formerly assigned to Sclerotinia Fuckel, but which differ from the true Sclerotinia species mainly in their erumpent, planoconvexoid sclerotia which are firmly attached to the substrate, and in the possession of a conidial state belonging to the form-genus Botrytis Pers. The type species is Botryotinia convoluta (Drayton) Whetzel in which the conidial state is a Botrytis of the cinerea type or B. cinerea sensu lato, and the other species included were Botryotinia fuckeliana (De Bary) Whetzel, of which the conidial state is Botrytis cinerea Pers. or B. cinerea sensu stricto, and Botryotinia ricini (Godfrey) Whetz. and B. porri (v. Beyma) Whetz. In the latter two species the conidial states would not be considered Botrytis species of the cinerea type. -
High-Level Classification of the Fungi and a Tool for Evolutionary Ecological Analyses
Fungal Diversity (2018) 90:135–159 https://doi.org/10.1007/s13225-018-0401-0 (0123456789().,-volV)(0123456789().,-volV) High-level classification of the Fungi and a tool for evolutionary ecological analyses 1,2,3 4 1,2 3,5 Leho Tedersoo • Santiago Sa´nchez-Ramı´rez • Urmas Ko˜ ljalg • Mohammad Bahram • 6 6,7 8 5 1 Markus Do¨ ring • Dmitry Schigel • Tom May • Martin Ryberg • Kessy Abarenkov Received: 22 February 2018 / Accepted: 1 May 2018 / Published online: 16 May 2018 Ó The Author(s) 2018 Abstract High-throughput sequencing studies generate vast amounts of taxonomic data. Evolutionary ecological hypotheses of the recovered taxa and Species Hypotheses are difficult to test due to problems with alignments and the lack of a phylogenetic backbone. We propose an updated phylum- and class-level fungal classification accounting for monophyly and divergence time so that the main taxonomic ranks are more informative. Based on phylogenies and divergence time estimates, we adopt phylum rank to Aphelidiomycota, Basidiobolomycota, Calcarisporiellomycota, Glomeromycota, Entomoph- thoromycota, Entorrhizomycota, Kickxellomycota, Monoblepharomycota, Mortierellomycota and Olpidiomycota. We accept nine subkingdoms to accommodate these 18 phyla. We consider the kingdom Nucleariae (phyla Nuclearida and Fonticulida) as a sister group to the Fungi. We also introduce a perl script and a newick-formatted classification backbone for assigning Species Hypotheses into a hierarchical taxonomic framework, using this or any other classification system. We provide an example -
The 100 Years of the Fungus Collection Mucl 1894-1994
THE 100 YEARS OF THE FUNGUS COLLECTION MUCL 1894-1994 Fungal Taxonomy and Tropical Mycology: Quo vadis ? Taxonomy and Nomenclature of the Fungi Grégoire L. Hennebert Catholic University of Louvain, Belgium Notice of the editor This document is now published as an archive It is available on www.Mycotaxon.com It is also produced on CD and in few paperback copies G. L. Hennebert ed. Published by Mycotaxon, Ltd. Ithaca, New York, USA December 2010 ISBN 978-0-930845-18-6 (www pdf version) ISBN 978-0-930845-17-9 (paperback version) DOI 10.5248/2010MUCL.pdf 1894-1994 MUCL Centenary CONTENTS Lists of participants 8 Forword John Webser 13 PLENARY SESSION The 100 Year Fungus Culture Collection MUCL, June 29th, 1994 G.L. Hennebert, UCL Mycothèque de l'Université Catholique de Louvain (MUCL) 17 D. Hawksworth, IMI, U.K. Fungal genetic resource collections and biodiversity. 27 D. van der Mei, CBS, MINE, Netherlands The fungus culture collections in Europe. 34 J. De Brabandere, BCCM, Belgium The Belgian Coordinated Collections of Microorganisms. 40 Fungal Taxonomy and tropical Mycology G.L. Hennebert, UCL Introduction. Fungal taxonomy and tropical mycology: Quo vadis ? 41 C.P. Kurtzman, NRRL, USA Molecular taxonomy in the yeast fungi: present and future. 42 M. Blackwell, Louisiana State University, USA Phylogeny of filamentous fungi deduced from analysis of molecular characters: present and future. 52 J. Rammeloo, National Botanical Garden, Belgium Importance of morphological and anatomical characters in fungal taxonomy. 57 M.F. Roquebert, Natural History Museum, France Possible progress of modern morphological analysis in fungal taxonomy. 63 A.J. -
Functional Analysis of Mating Type Genes and Transcriptome Analysis During Fruiting Body Development of Botrytis Cinerea
This is a repository copy of Functional Analysis of Mating Type Genes and Transcriptome Analysis during Fruiting Body Development of Botrytis cinerea. White Rose Research Online URL for this paper: http://eprints.whiterose.ac.uk/144689/ Version: Published Version Article: Rodenburg, S.Y.A., Terhem, R.B., Veloso, J. et al. (2 more authors) (2018) Functional Analysis of Mating Type Genes and Transcriptome Analysis during Fruiting Body Development of Botrytis cinerea. mBio, 9 (1). e01939-17. ISSN 2150-7511 https://doi.org/10.1128/mBio.01939-17 Reuse This article is distributed under the terms of the Creative Commons Attribution (CC BY) licence. This licence allows you to distribute, remix, tweak, and build upon the work, even commercially, as long as you credit the authors for the original work. More information and the full terms of the licence here: https://creativecommons.org/licenses/ Takedown If you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing [email protected] including the URL of the record and the reason for the withdrawal request. [email protected] https://eprints.whiterose.ac.uk/ RESEARCH ARTICLE crossm Functional Analysis of Mating Type Genes and Transcriptome Analysis during Fruiting Body Development of Botrytis cinerea Sander Y. A. Rodenburg,a,b Razak B. Terhem,a* Javier Veloso,a,c Joost H. M. Stassen,a* Jan A. L. van Kana aLaboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands bWageningen University, Bioinformatics Group, Wageningen, The Netherlands cDepartment of Plant Physiology, Faculty of Sciences, University of A Coruña, A Coruña, Spain ABSTRACT Botrytis cinerea is a plant-pathogenic fungus producing apothecia as sex- ual fruiting bodies. -
Botrytis Cinerea
Sexual development of Botrytis species Razak Bin Terhem Thesis committee Promotor Prof. Dr P.J.G.M de Wit Professor of Phytopathology Wageningen University Co-promotor Dr J.A.L. van Kan Assistant professor, Laboratory of Phytopathology Wageningen University Other members Prof. Dr T.W. Kuyper, Wageningen University Dr A.J.M. Debets, Wageningen University Prof. Dr H.A.B. Wösten, Utrecht University Dr G.H.J. Kema, Wageningen University This research was conducted under the auspices of the Graduate School of Experimental Plant Sciences Sexual development of Botrytis species Razak Bin Terhem Thesis submitted in fulfilment of the requirements for the degree of doctor at Wageningen University by the authority of the Rector Magnificus Prof. Dr A.P.J. Mol, in the presence of the Thesis Committee appointed by the Academic Board to be defended in public on Monday 14 September 2015 at 4 p.m. in the Aula. Razak Bin Terhem Sexual development of Botrytis species, 188 pages. PhD thesis, Wageningen University, Wageningen, NL (2015) With references, with summaries in English ISBN 978-94-6257-414-4 Contents Chapter 1 General introduction and outline of the thesis 7 Chapter 2 Transcriptome and functional analysis of fruiting 25 body development of Botrytis cinerea Chapter 3 Functional analysis of hydrophobin genes in sexual 87 development of Botrytis cinerea Chapter 4 Mating type and sexual fruiting body of Botrytis 111 elliptica, the causal agent of fire blight in lily Chapter 5 A novel Botrytis species is associated with a newly 131 emergent foliar disease in cultivated Hemerocallis Chapter 6 General discussion 159 Summary 179 Acknowledgements 181 Curriculum vitae 184 List of publications 185 Education statement of the graduate school 186 Chapter 1 General introduction and outline of the thesis Part of this chapter was published in: Kevin D. -
Fungicide Resistance in Botrytis Cinerea from Strawberry - Molecular Mechanisms and Management Anja Grabke Clemson University, [email protected]
Clemson University TigerPrints All Theses Theses 5-2014 Fungicide Resistance in Botrytis cinerea from Strawberry - Molecular Mechanisms and Management Anja Grabke Clemson University, [email protected] Follow this and additional works at: https://tigerprints.clemson.edu/all_theses Part of the Agronomy and Crop Sciences Commons, Biology Commons, and the Plant Pathology Commons Recommended Citation Grabke, Anja, "Fungicide Resistance in Botrytis cinerea from Strawberry - Molecular Mechanisms and Management" (2014). All Theses. 1922. https://tigerprints.clemson.edu/all_theses/1922 This Thesis is brought to you for free and open access by the Theses at TigerPrints. It has been accepted for inclusion in All Theses by an authorized administrator of TigerPrints. For more information, please contact [email protected]. FUNGICIDE RESISTANCE IN BOTRYTIS CINEREA FROM STRAWBERRY - MOLECULAR MECHANISMS AND MANAGEMENT A Thesis Presented to the Graduate School of Clemson University In Partial Fulfillment of the Requirements for the Degree Master of Science Plant and Environmental Sciences by Anja Grabke May 2014 Accepted by: Dr. Guido Schnabel, Committee Chair Dr. Paula Agudelo Dr. Julia Kerrigan ABSTRACT The United States is the largest producer of strawberries worldwide, generating approximately $2.4 billion worth of fruit each year. Strawberry production, however, is often threatened by pathogens. One of the most destructive fungal pathogens is Botrytis cinerea, the causal agent of gray mold disease. The control of gray mold in commercial fields is largely dependent on the application of fungicides, including the dicarboximide iprodione and the hydroxyanilide fenhexamid. Because both fungicides are prone to resistance development, a survey was conducted to determine the occurrence and prevalence of fungicide resistance. -
Drivers of Fungal Community Composition and Function In
DRIVERS OF FUNGAL COMMUNITY COMPOSITION AND FUNCTION IN TEMPERATE FORESTS A dissertation submitted to Kent State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy By Matthew D. Gacura December 2018 © Copyright All rights reserved Except for previously published materials i Dissertation written by Matthew David Gacura B.S., Youngstown State University, 2007 M.S., Youngstown State University, 2009 Ph.D., Kent State University, 2018 Approved by Christopher B. Blackwood, Ph.D. , Chair, Doctoral Dissertation Committee Mark W. Kershner, Ph.D. , Members, Doctoral Dissertation Committee Xiaozhen Mou, Ph.D. Mandy J. Munro-Stasiuk, Ph.D. Abdul Shakoor, Ph.D. Accepted by Laura G. Leff, Ph.D. , Chair, Department of Biological Sciences James L. Blank, Ph.D. , Dean, College of Arts and Sciences ii TABLE OF CONTENTS TABLE OF CONTENTS…………………………………………….…………………………...iii LIST OF FIGURES…………………………….………………….………………………………v LIST OF TABLES……………….………………………………………………………………..x ACKNOWLEDGEMENTS……………………………………………………………………...xii I. CHAPTER 1: INTRODUCTION………………………..……………………………1 REFERENCES……………………..………………………………………………..20 II. CHAPTER 2: NICHE VS NEUTRAL: FACTORS INFLUENCING THE STRUCTURE OF SAPROTROPHIC FUNGAL COMMUNITIES AT FINE AND LARGE SPATIAL SCALES……………...…………………………………………35 ABSTRACT………………………………………………………………………….35 INTRODUCTION…………………..……………………………………………….36 MATERIALS AND METHODS…………...……………………..…………………40 RESULTS……………………..……………………………………………………..47 DISCUSSION……………..……………………………………………………..…..51 ACKNOWLEDGEMENTS………………………………………………………….60 -
Botryotina and Botrytis Species
BOTRYOTINIA AND BOTRYTIS SPECIES taxonomy physiology and pathogenicity Monograph No. 15 1977 Agriculture Canada BOTRYOTINIA AND BOTRYTIS SPECIES: taxonomy, physiology, and pathogenicity A guide to the literature W. R. Jarvis Research Station Harrow, Ontario N0R 1G0 Research Branch Canada Department of Agriculture Monograph No. 15 1977 Copies of this publication may be obtained from INFORMATION DIVISION CANADA DEPARTMENT OF AGRICULTURE OTTAWA K1A 0C7 'MINISTER OF SUPPLY AND SERVICES CANADA 1977 2M-38745-7.77 Cat. No.: A54-3/15 ISBN No.: D-662-00794-8 Hignell Printing Limited Contract No.: 0KT7-1078 CONTENTS PART 1 Introduction 9 PART 2 Taxonomy 11 Taxonomic review 11 Sclerotiniaceae 11 Botryotinia 12 Botryotinia and Botrytis 13 Botrytis 14 The basis for classification 16 Numerical taxonomy 17 Biochemical differentiation 18 Variability 18 Sexual reproduction 18 PART 3 Form and Function 20 Anatomy and morphology 20 Mycelium 20 Conidiophores and conidia 20 Microconidia 21 Chlamydospores 22 Oidia 22 Haustoria 22 Appressoria 23 Organs of attachment 23 Sclerotia 23 Apothecia 25 Ultrastructure 25 Cytology 27 Variants 29 Adaptation to fungicides 31 Growth 33 Effects of temperature 33 Effects of relative humidity 34 Effects of light 35 Phototropism 38 Effects of pH 39 Effects of age and nutrition 39 Staling 41 Effects of volatile metabolites 42 Effects of atmospheric gases and pollutants 42 Autotropism 44 Rheotropism 44 Sporulation inhibitors 45 Storage 45 3 Metabolism 46 Selective media 46 Carbohydrates 46 Nitrogen 48 Amino acids and proteins 49 -
Table of Contents 1
Botrytis cinerea: mating types distribution and NEP1 protein sequence analysis TABLE OF CONTENTS 1. RESUMO ................................................................................................................................ 2 2. RESUMO ALARGADO ............................................................................................................ 3 3. ABSTRACT ............................................................................................................................. 6 4. lNTRODUCTION ..................................................................................................................... 7 4.1. THE PATHOGEN BOTRYTIS CINEREA ............................................................................ 8 4.2. SEXUAL REPRODUCTION AND MATING GENETICS .................................................... 11 4.2.1. BOTRYTIS CINEREA MATING SYSTEM ................................................................ 13 4.3. BOTRYTIS CINEREA GENETIC TRANSFORMATION ..................................................... 14 4.4. AIM OF THE RESEARCH ............................................................................................... 15 5. MATERIALS AND METHODS ............................................................................................... 16 5.1. MATING TYPE PCR-BASED SCREENING ...................................................................... 16 5.2. uORF PCR-BASED TARGETED MUTAGENESIS ............................................................. 17 5.3. NEP1 PROTEIN SEQUENCE STUDY -
Botryotinia Fuckeliana (De Bary) Whetzel Against Vinclozolin and the Phenylpyrrole CGA 173506
Comparative studies on genetic variability and fungicide resistance in Botryotinia fuckeliana (de Bary) Whetzel against vinclozolin and the phenylpyrrole CGA 173506 Inauguraldissertation zur Erlangung der Würde eines Doktors der Philosophie vorgelegt der Philosophisch- Naturwissenschaftlichen Fakultät der Universität Basel von Urs Werner Hilber aus Degersheim- Magdenau SG Basel, 1992 n BASLER - SCHNELLDRUCK : ~ BERNHARD SCHLATTMANN ~@Jm:.t-;(.!:...JURASTAA.SSE 5-5 TElefON 081 35 37 37 40 5 3 8ASEL Comparative studies on genetic variability and fungicide resistance in Botryotinia fuckeliana (de Bary) Whetzel against vinclozolin and the phenylpyrrole CGA 173506 Inauguraldissertation zur Erlangung der Würde eines Doktors der Philosophie vorgelegt der Philosophisch- Naturwissenschaftlichen Fakultät der Universität Basel von Urs Werner Hilber aus Degersheim- Magdenau SG Basel, 1992 Genehmigt von der Philosophisch- Naturwissenschaftlichen Fakultät auf Antrag der Herren Professoren Dr. F.J. Schwinn und Dr. T. Boiler Basel, den 28. April 1992 Prof. Dr. J.-M. Le Tensorer (Dekan) Dedicated to MajaBodmer Contents Preface 1 Disease cyde 2 Structure formulae 3 General Introduction 4 1. Field study on the population dynamics of Botryotinia fuckeliana (de Bary) Whetzel resistant to dicarboximides 8 1.1 lntroduction 8 1.2 Materialsand Methods 8 1.3 Results 10 1.4 Discussion 12 2. Studies on the base~line sensitivity of Botryotinia fuckeliana (de Bary) Whetzel against CGA 173506 15 1.1 Introduction 15 1.2 Materialsand Methods 15 1.3 Results 18 1.4 Discussion 22 3. Stu.dies on the occurrence of laboratory resistance to vindozolin and CGA 173506 in Botryotinia fuckeliana (de Bary) Whetzel and its influence on fitness parameters 24 1.1 Introduction 24 1.2 Materialsand Methods 25 1.3 Results 31 1.4 Discussion 47 4. -
Characterization of the Field Fludioxonil Resistance and Its Molecular Basis in Botrytis Cinerea from Shanghai Province in China
microorganisms Article Characterization of the Field Fludioxonil Resistance and Its Molecular Basis in Botrytis cinerea from Shanghai Province in China Weizhen Wang 1,†, Yuan Fang 1,†, Muhammad Imran 1 , Zhihong Hu 1, Sicong Zhang 1, Zhongqiao Huang 1 and Xili Liu 1,2,* 1 Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China; [email protected] (W.W.); [email protected] (Y.F.); [email protected] (M.I.); [email protected] (Z.H.); [email protected] (S.Z.); [email protected] (Z.H.) 2 State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712110, China * Correspondence: [email protected] † These authors equally contributed to this work. Abstract: Botrytis cinerea is a destructive necrotrophic pathogen that can infect many plant species. The control of gray mold mainly relies on the application of fungicides, and the fungicide fludioxonil is widely used in China. However, the field fungicide resistance of B. cinerea to this compound is largely unknown. In this study, B. cinerea isolates were collected from different districts of Shanghai province in 2015–2017, and their sensitivity to fludioxonil was determined. A total of 65 out of 187 field isolates (34.76%) were found to be resistant to fludioxonil, with 36 (19.25%) showing high resistance and 29 (15.51%) showing moderate resistance. Most of these resistant isolates also showed Citation: Wang, W.; Fang, Y.; Imran, resistance to iprodione, and some developed resistance to fungicides of other modes of action. AtrB M.; Hu, Z.; Zhang, S.; Huang, Z.; Liu, gene expression, an indicator of MDR1 and MDR1h phenotypes, was not dramatically increased in X. -
Managing Botrytis Bunch Rot Gray Mold Or Bunch Rot Can Cause Serious Yield Loss but There Are a Variety of Tools That Can Be Used to Protect Grapes
MICHIGAN Grape facts Managing botrytis bunch rot Gray mold or bunch rot can cause serious yield loss but there are a variety of tools that can be used to protect grapes. By Safa Alzohairy and Timothy Miles; MSU Department of Plant, Soil and Microbial Sciences Key messages » Avoidance – Avoid cluster damage from other pests such as insects, birds and other grape diseases. » Exclusion – Exclude the pathogen by harvesting in a timely fashion and increasing canopy airflow. » Resistance – Choose resistant varieties when possible. Safa Alzohairy, MSU » Protection – Protect flowers and clusters with Figure 1. Botrytis bunch rot caused by Botrytis cinerea (cv. Riesling). fungicides when the infection risk is high. » Eradication – Eradicate old canes and clusters Disease symptoms each season. In early spring, infected buds and young shoots will turn brown, while in late spring, V-shaped, large, Pathogen irregular brown areas appear on the leaves (Figure Botrytis cinerea 2A). Grape inflorescences may appear blighted when infected but flower infection mostly remains Kingdom: Fungi quiescent until veraison (Figure 2B). At veraison Division: Ascomycota and beyond, the fungus infects the clusters directly from the skin or through wounds caused by various Class: Leotiomycetes factors including bird claws or feeding, insects or Asexual form: Botrytis cinerea rain cracks. Sexual form: Botryotinia fuckeliana or also known as Infected berries can dry out in arid conditions while Botryotinia cinerea in wet weather, infected berries are covered with grayish mold. This mold contains fluffy mycelia and Introduction millions of spores or conidia. As infection proceeds, Botrytis cinerea, or the causal agent of gray mold and bunch rot, is one of the most important pathogens of grapes worldwide.