DOCSLIB.ORG

Seasonal Changes in Bacterioplankton Nutrient Limitation and Their Effects on Bacterial Community Composition in the NW Mediterranean Sea

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Seasonal Changes in Bacterioplankton Nutrient Limitation and Their Effects on Bacterial Community Composition in the NW Mediterranean Sea AQUATIC MICROBIAL ECOLOGY Vol. 44: 241–252, 2006 Published October 10 Aquat Microb Ecol OPENPEN ACCESSCCESS Seasonal changes in bacterioplankton nutrient limitation and their effects on bacterial community composition in the NW Mediterranean Sea Jarone Pinhassi1,*, Laura Gómez-Consarnau1, Laura Alonso-Sáez2, Maria Montserrat Sala2, Montserrat Vidal3, Carlos Pedrós-Alió2, Josep M. Gasol2 1Marine Microbiology, Department of Biology and Environmental Sciences, University of Kalmar, Barlastgatan 11, 39182 Kalmar, Sweden 2Institut de Ciències del Mar-CMIMA (CSIC), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Catalunya, Spain 3Departament d’Ecologia, Universitat de Barcelona, Avda. Diagonal 645, 08028 Barcelona, Catalunya, Spain ABSTRACT: Bacterioplankton growth is expected to depend on the availability of organic and in- organic nutrients. Still, no studies have investigated how the magnitude and type of nutrient limi- tation experienced by marine bacteria change on a temporal scale. We carried out a series of nutrient enrichment experiments to examine the variability in nutrient limitation of bacteria in the NW Mediterranean Sea, at monthly intervals, over an 18 mo period. Short-term enrichment bioassays (24 h incubation) showed that bacterial P limitation could occur throughout the year, but was most pronounced during spring and summer, coinciding with very low concentrations of dissolved in- organic phosphorus and chlorophyll a, and higher N:P ratios. During the non-stratified period in autumn and winter, bacteria were at times strongly C limited. Inorganic nitrogen limitation was not detected at any time. Long-term bioassays with and without enrichment, where growth was moni- tored until stationary phase using the seawater dilution culture approach, largely confirmed the results from the short-term bioassays. Analysis of the bacterial assemblages in these cultures, using denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene sequencing, suggested that the growth of some central components of the native bacterioplankton assemblage (i.e. specific Roseobacter and Flavobacteria phylotypes) was restricted due to the limited availability of P in spring and summer. We conclude that seasonal variability in the type and severity of nutrient limitation can substantially contribute to the regulation of bacterioplankton growth and community composition, and thereby affect the turnover of dissolved organic matter and inorganic nutrients in the sea. KEY WORDS: Marine bacteria · Nutrients · Bioassays · Seasonal variability · Diversity Resale or republication not permitted without written consent of the publisher INTRODUCTION dissolved inorganic nutrients in the photic zone of the sea are mainly regulated through physical mixing with Marine microbes experience seasonally changing deep water, e.g. autumn and winter overturn or growth conditions that eventually lead to differences upwelling, leading to increased concentrations and in the structuring of community composition over sea- biological consumption by microorganisms that leads sonal scales. Changes in inorganic nutrient availability to their depletion. The variation in nutrient regimes are of decisive importance for defining ocean primary affects phytoplankton production, diversity and suc- productivity and for regulating phytoplankton commu- cession (Smayda & Reynolds 2001, 2003), but an nity composition and succession. The concentrations of understanding of how seasonal changes in nutrient *Email: [email protected] © Inter-Research 2006 · www.int-res.com 242 Aquat Microb Ecol 44: 241–252, 2006 availability affect bacterioplankton heterotrophic pro- different phytoplankton, indicating that dissolved duction and community composition is still lacking. organic matter from different algae select for different The primary limiting nutrient for bacterial growth bacteria (Pinhassi et al. 2004, Abell & Bowman 2005, differs between sea areas. It is generally assumed that Grossart et al. 2005). Further, experiments with estuarine growth of bacterioplankton in the Mediterranean Sea bacterioplankton show that different complex dissolved is limited by the availability of inorganic P (Zweifel et organic carbon sources selectively stimulate the al. 1993, Thingstad et al. 1998, 2005, Zohary & Robarts growth of specific phylotypes (Covert & Moran 2001, 1998, Sala et al. 2002, Tanaka et al. 2004, Pitta et al. Kisand & Wikner 2003). In North Sea and east Mediter- 2005), while, for example, the availability of organic C ranean seawater dilution cultures, enrichment with eas- or inorganic P or Fe can limit the growth of bacteria in ily degradable carbon sources (e.g. glucose) favored the the subarctic Pacific, the Sargasso Sea or Antarctica growth of Vibrio species (Eilers et al. 2000, Pinhassi & (Kirchman 1990, Pakulski et al. 1996, Cotner et al. Berman 2003). Also, the growth of Gammaproteobac- 1997, Rivkin & Anderson 1997). Considering the often teria was preferentially stimulated by Fe enrichment in large seasonal fluctuations in nutrient concentrations different seas where bacterial growth was limited by Fe in the sea, variability in the type of bacterial nutrient availability (Hutchins et al. 2001). These studies focused limitation can be expected (Sala et al. 2002). Indeed, it mainly on the selective role of organic matter on the has been speculated that inconsistent findings of C or P growth of specific bacteria, but also considered aspects limitation in the Sargasso Sea may be explained by of inorganic nutrient availability. Taken together, they temporal variability (Cotner et al. 1997, Carlson et al. suggest that changes in the availability of both organic 2002, 2004). Studies in freshwater environments have and inorganic nutrients could be important for defining shown substantial seasonal variability in bacterio- the composition of bacterial communities in the sea. plankton nutrient limitation in relation to nutrient In the present study, we carried out a series of nutri- availability, with bacterial growth responding primar- ent enrichment experiments to examine the extent of ily to P or combined P and C enrichments (Morris & organic and/or inorganic nutrient limitation of marine Lewis 1992, Carlsson & Caron 2001). However, in the bacterioplankton in a defined coastal marine area over sea, little is known about the temporal variability in the an 18 mo period. To estimate nutrient limitation, we kind and extent of nutrient limitation experienced by used 2 complementary bioassay approaches that are bacterioplankton and its consequences in terms of frequently used to investigate bacterial growth char- regulating bacterial growth. acteristics (see Pinhassi & Berman 2003 for details Because heterotrophic bacteria are the major con- of methodologies). Short-term enrichment bioassays sumers of dissolved organic matter in the aquatic envi- (24 h incubation) were used to estimate the immediate ronment, limitation of bacterial growth by organic or growth response of the bacteria. Long-term bioassays, inorganic nutrients can have important consequences whereby growth was monitored until stationary phase in terms of biogeochemical C cycling. For example, using the seawater dilution culture approach, allowed modeling, experimental, and in situ results all show us to evaluate how differences in nutrient availability that labile DOC can accumulate when bacteria are lim- affected particular members of the bacterioplankton ited by inorganic nutrients (Zweifel et al. 1995, assemblage. It was hypothesized that differences in Thingstad et al. 1997, 1999), which may occur for the composition of the bacterial assemblage develop- example in the P limited Baltic Sea in summer (Zweifel ing in seawater cultures with and without nutrient et al. 1995). In the Sargasso Sea, DOC also accumu- enrichment would depend on the type and extent of in lates in summer (Carlson et al. 1994, 2002). In this sea, situ nutrient limitation. however, bacteria are limited by the availability of organic C in summer, and labile DOC is continuously taken up by bacteria while semilabile DOC resistant to MATERIALS AND METHODS rapid degradation by bacteria accumulates (Carlson et al. 2002, 2004). Thus, bacterial nutrient limitation may In situ sampling. NW Mediterranean Sea water from define whether DOC accumulated in surface waters is 0.5 m depth was collected at monthly intervals labile or not. This has important consequences in the between 28 January 2003 and 19 July 2004 (see long term for defining the quality of DOC that is Table 1), 1 km off the coast in the Bay of Blanes (The exported to deep waters by convective processes Blanes Bay Microbial Observatory, 41° 40’ N, 2° 48’ E, (Murray et al. 1994) and in the short term for determin- 60 km north of Barcelona, Spain). Water was filtered ing the ability of bacteria to take advantage of pulses of through a 200 µm mesh net, collected in a 25 l polycar- inorganic nutrients (Hagström et al. 2001). bonate bottle (Nalgene), and brought to the laboratory Bacterioplankton community structure changes re- for processing within 3 h. For convenience, the sam- latively fast in response to the growth and decay of pling on 4 March 2003 is referred to as ‘February Pinhassi et al.: Seasonality in bacterioplankton nutrient limitation 243 2003’, to avoid confusion with the sampling on 25 the leucine incorporation data. Such delays in increase March 2003 (i.e. ‘March 2003’). in bacterial abundance compared to leucine incor- Chlorophyll a (chl a). Chl a was measured fluoro- poration
Load more

Recommended publications
  • Horizontal Operon Transfer, Plasmids, and the Evolution of Photosynthesis in Rhodobacteraceae
    The ISME Journal (2018) 12:1994–2010 https://doi.org/10.1038/s41396-018-0150-9 ARTICLE Horizontal operon transfer, plasmids, and the evolution of photosynthesis in Rhodobacteraceae 1 2 3 4 1 Henner Brinkmann ● Markus Göker ● Michal Koblížek ● Irene Wagner-Döbler ● Jörn Petersen Received: 30 January 2018 / Revised: 23 April 2018 / Accepted: 26 April 2018 / Published online: 24 May 2018 © The Author(s) 2018. This article is published with open access Abstract The capacity for anoxygenic photosynthesis is scattered throughout the phylogeny of the Proteobacteria. Their photosynthesis genes are typically located in a so-called photosynthesis gene cluster (PGC). It is unclear (i) whether phototrophy is an ancestral trait that was frequently lost or (ii) whether it was acquired later by horizontal gene transfer. We investigated the evolution of phototrophy in 105 genome-sequenced Rhodobacteraceae and provide the first unequivocal evidence for the horizontal transfer of the PGC. The 33 concatenated core genes of the PGC formed a robust phylogenetic tree and the comparison with single-gene trees demonstrated the dominance of joint evolution. The PGC tree is, however, largely incongruent with the species tree and at least seven transfers of the PGC are required to reconcile both phylogenies. 1234567890();,: 1234567890();,: The origin of a derived branch containing the PGC of the model organism Rhodobacter capsulatus correlates with a diagnostic gene replacement of pufC by pufX. The PGC is located on plasmids in six of the analyzed genomes and its DnaA- like replication module was discovered at a conserved central position of the PGC. A scenario of plasmid-borne horizontal transfer of the PGC and its reintegration into the chromosome could explain the current distribution of phototrophy in Rhodobacteraceae.
    [Show full text]
  • Draft Genomic Sequence of Nereida Ignava CECT 5292T, a Marine Bacterium of the Family Rhodobacteraceae David R
    Arahal et al. Standards in Genomic Sciences (2016) 11:21 DOI 10.1186/s40793-016-0141-2 SHORT GENOME REPORT Open Access Draft genomic sequence of Nereida ignava CECT 5292T, a marine bacterium of the family Rhodobacteraceae David R. Arahal*, María J. Pujalte and Lidia Rodrigo-Torres Abstract Nereida ignava strain 2SM4T (= CECT 5292T = DSM 16309T = CIP 108404T = CCUG 49433T) is a marine bacterium belonging to the Roseobacter group of the family Rhodobacteraceae within the class Alphaproteobacteria. The strain was isolated from sea water surrounding cultivated oysters 2–3 miles off the Mediterranean coast near Valencia (Spain) and was phylogenetically related to uncultured clones of gall symbiont bacteria of some species of Prionitis alga. Here we describe the genome sequence and annotation of this organism, the type strain of the single species of this genus. The genome comprised 2,888,349 bp, 2,872 protein-coding genes and 52 RNA genes. The annotation revealed the capacity to produce bacteriocins, vitamins and auxins. Besides, it contained sulfur cycling related genes. Keywords: Nereida ignava, Rhodobacteraceae, Mediterranean Sea, Strictly aerobic, Slightly halophilic, Marine bacteria Introduction of the red algal genus Prionitis [1]. Gall formation in this Nereida is a genus of the Roseobacter group, within the alga was reported to be induced by bacteria phylogenet- family Rhodobacteraceae, order Rhodobacterales, Class ically related to the Roseobacter group [6, 7]. It is known Alphaproteobacteria, so far containing only one species, that this relationship is species specific and coevolution Nereida ignava [1]. At the time of writing there are 197 exists between host and bacteria [8].
    [Show full text]
  • Photosynthesis Is Widely Distributed Among Proteobacteria As Demonstrated by the Phylogeny of Puflm Reaction Center Proteins
    fmicb-08-02679 January 20, 2018 Time: 16:46 # 1 ORIGINAL RESEARCH published: 23 January 2018 doi: 10.3389/fmicb.2017.02679 Photosynthesis Is Widely Distributed among Proteobacteria as Demonstrated by the Phylogeny of PufLM Reaction Center Proteins Johannes F. Imhoff1*, Tanja Rahn1, Sven Künzel2 and Sven C. Neulinger3 1 Research Unit Marine Microbiology, GEOMAR Helmholtz Centre for Ocean Research, Kiel, Germany, 2 Max Planck Institute for Evolutionary Biology, Plön, Germany, 3 omics2view.consulting GbR, Kiel, Germany Two different photosystems for performing bacteriochlorophyll-mediated photosynthetic energy conversion are employed in different bacterial phyla. Those bacteria employing a photosystem II type of photosynthetic apparatus include the phototrophic purple bacteria (Proteobacteria), Gemmatimonas and Chloroflexus with their photosynthetic relatives. The proteins of the photosynthetic reaction center PufL and PufM are essential components and are common to all bacteria with a type-II photosynthetic apparatus, including the anaerobic as well as the aerobic phototrophic Proteobacteria. Edited by: Therefore, PufL and PufM proteins and their genes are perfect tools to evaluate the Marina G. Kalyuzhanaya, phylogeny of the photosynthetic apparatus and to study the diversity of the bacteria San Diego State University, United States employing this photosystem in nature. Almost complete pufLM gene sequences and Reviewed by: the derived protein sequences from 152 type strains and 45 additional strains of Nikolai Ravin, phototrophic Proteobacteria employing photosystem II were compared. The results Research Center for Biotechnology (RAS), Russia give interesting and comprehensive insights into the phylogeny of the photosynthetic Ivan A. Berg, apparatus and clearly define Chromatiales, Rhodobacterales, Sphingomonadales as Universität Münster, Germany major groups distinct from other Alphaproteobacteria, from Betaproteobacteria and from *Correspondence: Caulobacterales (Brevundimonas subvibrioides).
    [Show full text]
  • CGM-18-001 Perseus Report Update Bacterial Taxonomy Final Errata
    report Update of the bacterial taxonomy in the classification lists of COGEM July 2018 COGEM Report CGM 2018-04 Patrick L.J. RÜDELSHEIM & Pascale VAN ROOIJ PERSEUS BVBA Ordering information COGEM report No CGM 2018-04 E-mail: [email protected] Phone: +31-30-274 2777 Postal address: Netherlands Commission on Genetic Modification (COGEM), P.O. Box 578, 3720 AN Bilthoven, The Netherlands Internet Download as pdf-file: http://www.cogem.net → publications → research reports When ordering this report (free of charge), please mention title and number. Advisory Committee The authors gratefully acknowledge the members of the Advisory Committee for the valuable discussions and patience. Chair: Prof. dr. J.P.M. van Putten (Chair of the Medical Veterinary subcommittee of COGEM, Utrecht University) Members: Prof. dr. J.E. Degener (Member of the Medical Veterinary subcommittee of COGEM, University Medical Centre Groningen) Prof. dr. ir. J.D. van Elsas (Member of the Agriculture subcommittee of COGEM, University of Groningen) Dr. Lisette van der Knaap (COGEM-secretariat) Astrid Schulting (COGEM-secretariat) Disclaimer This report was commissioned by COGEM. The contents of this publication are the sole responsibility of the authors and may in no way be taken to represent the views of COGEM. Dit rapport is samengesteld in opdracht van de COGEM. De meningen die in het rapport worden weergegeven, zijn die van de auteurs en weerspiegelen niet noodzakelijkerwijs de mening van de COGEM. 2 | 24 Foreword COGEM advises the Dutch government on classifications of bacteria, and publishes listings of pathogenic and non-pathogenic bacteria that are updated regularly. These lists of bacteria originate from 2011, when COGEM petitioned a research project to evaluate the classifications of bacteria in the former GMO regulation and to supplement this list with bacteria that have been classified by other governmental organizations.
    [Show full text]
  • Appendix 1. Validly Published Names, Conserved and Rejected Names, And
    Appendix 1. Validly published names, conserved and rejected names, and taxonomic opinions cited in the International Journal of Systematic and Evolutionary Microbiology since publication of Volume 2 of the Second Edition of the Systematics* JEAN P. EUZÉBY New phyla Alteromonadales Bowman and McMeekin 2005, 2235VP – Valid publication: Validation List no. 106 – Effective publication: Names above the rank of class are not covered by the Rules of Bowman and McMeekin (2005) the Bacteriological Code (1990 Revision), and the names of phyla are not to be regarded as having been validly published. These Anaerolineales Yamada et al. 2006, 1338VP names are listed for completeness. Bdellovibrionales Garrity et al. 2006, 1VP – Valid publication: Lentisphaerae Cho et al. 2004 – Valid publication: Validation List Validation List no. 107 – Effective publication: Garrity et al. no. 98 – Effective publication: J.C. Cho et al. (2004) (2005xxxvi) Proteobacteria Garrity et al. 2005 – Valid publication: Validation Burkholderiales Garrity et al. 2006, 1VP – Valid publication: Vali- List no. 106 – Effective publication: Garrity et al. (2005i) dation List no. 107 – Effective publication: Garrity et al. (2005xxiii) New classes Caldilineales Yamada et al. 2006, 1339VP VP Alphaproteobacteria Garrity et al. 2006, 1 – Valid publication: Campylobacterales Garrity et al. 2006, 1VP – Valid publication: Validation List no. 107 – Effective publication: Garrity et al. Validation List no. 107 – Effective publication: Garrity et al. (2005xv) (2005xxxixi) VP Anaerolineae Yamada et al. 2006, 1336 Cardiobacteriales Garrity et al. 2005, 2235VP – Valid publica- Betaproteobacteria Garrity et al. 2006, 1VP – Valid publication: tion: Validation List no. 106 – Effective publication: Garrity Validation List no. 107 – Effective publication: Garrity et al.
    [Show full text]
  • HASELHORST-THESIS.Pdf (2.779Mb)
    TYPICAL COLLEGE STUDENT DIET FOOD DERIVED MICROORGANISMS AND THEIR RELATION TO THE HUMAN GASTROINTESTINAL MICROFLORA A Senior Scholar Thesis by ALEXANDRIA JOANN HASELHORST Submitted to the Office of Undergraduate Research Texas A&M University in partial fulfillment of the requirements for the designation as UNDERGRADUATE RESEARCH SCHOLAR April 2010 Major: Nutritional Sciences TYPICAL COLLEGE STUDENT DIET FOOD DERIVED MICROORGANISMS AND THEIR RELATION TO THE HUMAN GASTROINTESTINAL MICROFLORA A Senior Scholar Thesis by ALEXANDRIA JOANN HASELHORST Submitted to the Office of Undergraduate Research Texas A&M University in partial fulfillment of the requirements for the designation as UNDERGRADUATE RESEARCH SCHOLAR Approved by: Research Advisor: Dr. Suresh Pillai Associate Dean for Undergraduate Research: Robert C. Webb April 2010 Major: Nutritional Sciences iii ABSTRACT Typical College Student Diet Food Derived Microorganisms and Their Relation to the Human Gastrointestinal Microflora. (April 2010) Alexandria Joann Haselhorst Department of Nutrition Texas A&M University Research Advisor: Dr. Suresh Pillai Department of Poultry Science & Nutrition and Food Science Current research in the field of obesity has shown that obese people generally have a higher percentage of Firmicutes compared to Bacteroidetes in their gastrointestinal tracts than lean people. The goal of this study was to assess whether or not different foods contain unique bacterial profiles and if there is a correlation between foods and the types of bacteria that may be present. This study was based on diets that are typically consumed by college students. Six meals, two breakfasts, two lunches, and two dinners were created based off a class project that detailed the diet of Texas A&M University students.
    [Show full text]
  • Supplementary Fig. 5
    PFspades_M3_96_0.952241;Bacteria;Proteobacteria;Alphaproteobacteria;Rickettsiales;Mitochondria;NA Matam_M3_6315;Bacteria;Proteobacteria;Alphaproteobacteria;Rickettsiales;Mitochondria;NA PFspades_M3_102_1.274286;Bacteria;Proteobacteria;Alphaproteobacteria;Rickettsiales;Mitochondria;NA PFspades_M2_93_1.298496;Bacteria;Proteobacteria;Alphaproteobacteria;Rickettsiales;Mitochondria;NA Matam_M4_5440;Bacteria;Proteobacteria;Alphaproteobacteria;Rickettsiales;Mitochondria;NAPFspades_M4_75_1.305007;Bacteria;Proteobacteria;Alphaproteobacteria;Rickettsiales;Mitochondria;NA 0.999 A Matam_M3_6487;Bacteria;Proteobacteria;Alphaproteobacteria;Rickettsiales;Mitochondria;NA PFspades_M2_97_0.823083;Bacteria;Proteobacteria;Alphaproteobacteria;Rickettsiales;Mitochondria;NA 0.841 0.876 Matam_M3_5437;Bacteria;Proteobacteria;Gammaproteobacteria;SAR86Matam_M3_5438;Bacteria;Proteobacteria;Gammaproteobacteria;SAR86 clade;NA;NA clade;NA;NA 0.787 Matam_M2_1930;Bacteria;Proteobacteria;Gammaproteobacteria;SAR86 clade;NA;NA Matam_M3_3768;Bacteria;Proteobacteria;Gammaproteobacteria;SAR86 clade;NA;NA Matam_M3_1904;Bacteria;Proteobacteria;Gammaproteobacteria;SAR86 clade;NA;NA Matam_M2_1678;Bacteria;Proteobacteria;Gammaproteobacteria;SAR86 clade;NA;NA Matam_M2_1258;Bacteria;Proteobacteria;Alphaproteobacteria;NA;NA;NA Matam_M4_1919;Bacteria;Proteobacteria;Gammaproteobacteria;SAR86 clade;NA;NA Matam_M3_1916;Bacteria;Proteobacteria;Gammaproteobacteria;SAR86 clade;NA;NA 1.000 Matam_M2_1974;Bacteria;Proteobacteria;Gammaproteobacteria;SAR86 clade;NA;NA Matam_M2_1403;Bacteria;Proteobacteria;Gammaproteobacteria;Alteromonadales;Pseudoalteromonadaceae;Pseudoalteromonas
    [Show full text]
  • Beneckea PAUL BAUMANN, LINDA BAUMANN, and M
    JOURNAL OF BACTERIOLOGY, JUly 1971, P. 268-294 Vol. 107, No. 1 Copyright 0 1971 American Society for Microbiology Printed in U.S.A. Taxonomy of Marine Bacteria: the Genus Beneckea PAUL BAUMANN, LINDA BAUMANN, AND M. MANDEL Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822, and Department of Biology, The University of Texas, M. D. Anderson Hospital and Tumor Institute at Houston, Houston, Texas 77025 Received for publication 5 April 1971 One-hundred-and-forty-five isolates of marine origin were submitted to an exten- sive physiological, nutritional, and morphological characterization. All strains were gram-negative, facultatively anaerobic, straight or curved rods which were motile by means of flagella. Glucose was fermented with the production of acid but no gas. Sodium but no organic growth factors were required. None of the strains were able to denitrify or fix molecular nitrogen. The results of nutritional and physiolog- ical tests were submitted to a numerical analysis. On the basis of phenotypic simi- larity, nine groups were established. These groups could be distinguished from one another by multiple, unrelated, phenotypic traits. Six groups which had deoxyri- bonucleic acid (DNA) containing 45 to 48 moles per cent guanine plus cytosine (GC) were assigned to a redefined genus Beneckea. All of the strains in this genus, when grown in liquid medium, had a single, polar flagellum. When grown on a solid medium, many strains had peritrichous flagella. Two groups were similar to previously described species and were designated B. alginolytica and B. natriegens. The remaining four groups were designated B. campbellii, B. neptuna, B.
    [Show full text]
  • Photoreactivity of Riverine and Phytoplanktonic Dissolved Organic
    Photoreactivity of riverine and phytoplanktonic dissolved organic matter and its effects on the dynamics of a bacterial community from the coastal Mediterranean Sea Marine Blanchet, Camila Fernandez, Fabien Joux To cite this version: Marine Blanchet, Camila Fernandez, Fabien Joux. Photoreactivity of riverine and phytoplanktonic dissolved organic matter and its effects on the dynamics of a bacterial community from the coastal Mediterranean Sea. Progress in Oceanography, Elsevier, 2017, 10.1016/j.pocean.2017.03.003. hal- 01490486 HAL Id: hal-01490486 https://hal.sorbonne-universite.fr/hal-01490486 Submitted on 15 Mar 2017 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Revised version PROOCE_2016_165 – modifications are underlined Photoreactivity of riverine and phytoplanktonic dissolved organic matter and its effects on the dynamics of a bacterial community from the coastal Mediterranean Sea Marine Blanchet, Camila Fernandez, Fabien Joux* Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire d'Océanographie Microbienne (LOMIC), Observatoire Océanologique, F-66650, Banyuls/mer, France * corresponding author: [email protected] For submission to Progress in Oceanography Special issue MERMEX as full length research paper Abstract High solar radiation levels measured in the Mediterranean basin can result in photo-oxidation of dissolved organic matter (DOM) at the sea surface.
    [Show full text]
  • Tree Scale: 1 0.49 Bchy D Bacteria P Proteobacteria C Alphaproteobacteria O Rhodobacterales F Rhodobacteraceae G Maliponia S Maliponia Aquimaris WGS ID FXYF01 1/1-387
    bchY d Bacteria p Proteobacteria c Alphaproteobacteria o Rhodobacterales f Rhodobacteraceae g Rhodosalinus s Rhodosalinus sp003298775 WGS ID QNTQ01 1/1 389 0.54 bchY d Bacteria p Proteobacteria c Alphaproteobacteria o Rhodobacterales f Rhodobacteraceae g Pseudooctadecabacter s Pseudooctadecabacter jejudonensis WGS ID FWFT01 1/1-389 0.49 bchY GCA 000014045.1 ASM1404v1 protein ABG29845.1 chlorophyllide reductase subunit Y Roseobacter denitrificans OCh 114 /1-389 0.22bchY d Bacteria p Proteobacteria c Alphaproteobacteria o Rhodobacterales f Rhodobacteraceae g Ascidiaceihabitans s Ascidiaceihabitans donghaensis WGS ID OMOR01 1/1-389 Tree scale: 1 0.49 bchY d Bacteria p Proteobacteria c Alphaproteobacteria o Rhodobacterales f Rhodobacteraceae g Maliponia s Maliponia aquimaris WGS ID FXYF01 1/1-387 0.52 bchY d Bacteria p Proteobacteria c Alphaproteobacteria o Rhodobacterales f Rhodobacteraceae g Nereida s Nereida ignava WGS ID FORZ01 1/1-390 0.59 bchY d Bacteria p Proteobacteria c Alphaproteobacteria o Rhodobacterales f Rhodobacteraceae g Lutimaribacter s Lutimaribacter saemankumensis WGS ID FNEB01 1/1-389 0.89 bchY d Bacteria p Proteobacteria c Alphaproteobacteria o Rhodobacterales f Rhodobacteraceae g Pseudaestuariivita s Pseudaestuariivita atlantica WGS ID AQQZ01 1/1-390 0.46 bchY d Bacteria p Proteobacteria c Alphaproteobacteria o Rhodobacterales f Rhodobacteraceae g Jannaschia s Jannaschia seohaensis WGS ID QGDJ01 1/1-390 0.17 bchY d Bacteria p Proteobacteria c Alphaproteobacteria o Rhodobacterales f Rhodobacteraceae g Pontivivens s Pontivivens
    [Show full text]
  • From a Basic Microalga and an Acetic Acid Bacterium Cellulose Producer to a Living Symbiotic Biofilm
    materials Article From a Basic Microalga and an Acetic Acid Bacterium Cellulose Producer to a Living Symbiotic Biofilm Vítor Nóbrega 1, Marisa Faria 1,2, Antera Quintana 3, Manfred Kaufmann 4,5 , Artur Ferreira 6 and Nereida Cordeiro 1,5,* 1 LB3, Faculty of Science and Engineering, University of Madeira, 9000-390 Funchal, Portugal 2 Oceanic Observatory of Madeira (OOM), ARDITI, Madeira Tecnopolo, 9020-105 Funchal, Portugal 3 Banco Español de Algas, Instituto de Oceanografía y Cambio Global (IOCAG), Universidad de Las Palmas de Gran Canaria, 35214 Telde, Spain 4 Marine Biology Station of Funchal, Faculty of Life Sciences, University of Madeira, 9000-107 Funchal, Portugal 5 CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Porto, 4450-208 Matosinhos, Portugal 6 CICECO, Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal * Correspondence: ncordeiro@staff.uma.pt Received: 10 June 2019; Accepted: 10 July 2019; Published: 15 July 2019 Abstract: Bacterial cellulose (BC) has recently been the subject of a considerable amount of research, not only for its environmentally friendly biosynthesis, but also for its high potential in areas such as biomedicine or biomaterials. A symbiotic relationship between a photosynthetic microalga, Chlamydomonas debaryana, and a cellulose producer bacterium, Komagataeibacter saccharivorans, was established in order to obtain a viable and active biofilm. The effect of the growth media composition ratio on the produced living material was investigated, as well as the microalgae biomass quantity, temperature, and incubation time. The optimal temperature for higher symbiotic biofilm production was 30 ◦C with an incubation period of 14 days. The high microalgae presence, 0.75% w/v, and 60:40 HS:BG-11 medium (v/v) induced a biofilm microalgae incorporation rate of 85%.
    [Show full text]
  • Photosynthesis Is Widely Distributed Among Proteobacteria As Demonstrated by the Phylogeny of Puflm Reaction Center Proteins
    fmicb-08-02679 January 20, 2018 Time: 16:46 # 1 ORIGINAL RESEARCH published: 23 January 2018 doi: 10.3389/fmicb.2017.02679 Photosynthesis Is Widely Distributed among Proteobacteria as Demonstrated by the Phylogeny of PufLM Reaction Center Proteins Johannes F. Imhoff1*, Tanja Rahn1, Sven Künzel2 and Sven C. Neulinger3 1 Research Unit Marine Microbiology, GEOMAR Helmholtz Centre for Ocean Research, Kiel, Germany, 2 Max Planck Institute for Evolutionary Biology, Plön, Germany, 3 omics2view.consulting GbR, Kiel, Germany Two different photosystems for performing bacteriochlorophyll-mediated photosynthetic energy conversion are employed in different bacterial phyla. Those bacteria employing a photosystem II type of photosynthetic apparatus include the phototrophic purple bacteria (Proteobacteria), Gemmatimonas and Chloroflexus with their photosynthetic relatives. The proteins of the photosynthetic reaction center PufL and PufM are essential components and are common to all bacteria with a type-II photosynthetic apparatus, including the anaerobic as well as the aerobic phototrophic Proteobacteria. Edited by: Therefore, PufL and PufM proteins and their genes are perfect tools to evaluate the Marina G. Kalyuzhanaya, phylogeny of the photosynthetic apparatus and to study the diversity of the bacteria San Diego State University, United States employing this photosystem in nature. Almost complete pufLM gene sequences and Reviewed by: the derived protein sequences from 152 type strains and 45 additional strains of Nikolai Ravin, phototrophic Proteobacteria employing photosystem II were compared. The results Research Center for Biotechnology (RAS), Russia give interesting and comprehensive insights into the phylogeny of the photosynthetic Ivan A. Berg, apparatus and clearly define Chromatiales, Rhodobacterales, Sphingomonadales as Universität Münster, Germany major groups distinct from other Alphaproteobacteria, from Betaproteobacteria and from *Correspondence: Caulobacterales (Brevundimonas subvibrioides).
    [Show full text]