A peer-reviewed open-access journal ZooKeys 878:Phylogenetic 1–22 (2019) relationships and subgeneric classification of EuropeanEphedrus species 1 doi: 10.3897/zookeys.878.38408 RESEARCH ARTICLE http://zookeys.pensoft.net Launched to accelerate biodiversity research Phylogenetic relationships and subgeneric classification of European Ephedrus species (Hymenoptera, Braconidae, Aphidiinae) Korana Kocić1, Andjeljko Petrović1, Jelisaveta Čkrkić1, Milana Mitrović2, Željko Tomanović1 1 University of Belgrade-Faculty of Biology, Institute of Zoology. Studentski Trg 16, 11000 Belgrade, Serbia 2 Institute for Plant Protection and Environment, Department of Plant Pests, Banatska 33, 11000 Belgrade, Serbia Corresponding author: Korana Kocić (
[email protected]) Academic editor: K. van Achterberg | Received 21 July 2019 | Accepted 2 September 2019 | Published 7 October 2019 http://zoobank.org/9B51B440-ACFC-4E1A-91EA-32B28554AF56 Citation: Kocić K, Petrović A, Čkrkić J, Mitrović M, Tomanović Ž (2019) Phylogenetic relationships and subgeneric classification of EuropeanEphedrus species (Hymenoptera, Braconidae, Aphidiinae). ZooKeys 878: 1–22. https://doi. org/10.3897/zookeys.878.38408 Abstract In this study two molecular markers were used to establish taxonomic status and phylogenetic relation- ships of Ephedrus subgenera and species distributed in Europe. Fifteen of the nineteen currently known species have been analysed, representing three subgenera: Breviephedrus Gärdenfors, 1986, Lysephedrus Starý, 1958 and Ephedrus Haliday, 1833. The results of analysis of COI and EF1α molecular markers and morphological studies did not support this classification. Three clades separated by the highest genetic distances reported for the subfamily Aphidiinae on intrageneric level. Ephedrus brevis is separated from persicae and plagiator species groups with genetic distances of 19.6 % and 16.3 % respectively, while the distance between persicae and plagiator groups was 20.7 %.