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Validation Report: ELISA SRA 36600 • Angelonia Flower Break Virus (Anfbv)

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Validation Report: ELISA SRA 36600 • Angelonia Flower Break Virus (Anfbv) Validation Report: ELISA SRA 36600 • Angelonia flower break virus (AnFBV) Test Characteristics Test Name Angelonia flower break virus Capture Antibody Polyclonal (Rabbit) Catalog Number 36600 Detection Antibody Polyclonal (Rabbit) Acronym AnFBV Format DAS-ELISA Genus Carmovirus Diluents GEB/RUB6 Sample Dilution 1:10 Summary This ELISA test is a qualitative serological assay for the detection of Angelonia flower break virus (AnFBV) in ornamental leaves. AnFBV is a member of the Alphacarmovirus genus known for their non-enveloped, spherical, icosahedral-shaped virus particles. Diagnostic Sensitivity Analytical Sensitivity True Positives 20 Limit of Detection: 1:8,100 dilution of infected tissue (pathogen titer unknown) Correct Diagnoses 20 Percent 100% Analytical Specificity Inclusivity: Isolates and Geographic Regions Detected: AnFBV Germany isolate AnFBV Israel isolate AnFBV USA isolate Exclusivity: Cross-reacts With: None known Does Not Cross-react With: Alfalfa mosaic virus (AMV) Arabis mosaic virus (ArMV) Bean common mosaic virus (BCMV) Bean pod mottle virus (BPMV) Broad bean wilt virus 1 and 2 (BBWV-1,2) Calibrachoa mottle virus (CbMV) Carnation mottle virus (CarMV) Cucumber mosaic virus (CMV) Cucumber mosaic virus I (CMV I) Cucumber mosaic virus II (CMV II) Chrysanthemum virus B (CVB) Hibiscus chlorotic ringspot virus (HCRSV) Impatiens necrotic spot virus (INSV) Lettuce mosaic virus (LMV) Melon necrotic spot virus (MNSV) Nemesia ringspot virus (NeRNV) Pelargonium flower break virus (PFBV) Prunus necrotic ringspot virus (PNRSV) Agdia, Inc. 52642 County Road 1 Elkhart, IN 46514 p147.2 574-264-2014 / 800-622-4342 Revised: 09/07/2021 www.agdia.com / [email protected] Page 1 of 3 Does Not Cross-react With: Potyvirus group (Poty) Peanut stunt virus (PSV) Potato virus Y (PVY) Ribgrass mosaic virus (RMV) Tobacco mosaic virus (TMV) Tobacco mosaic virus, common strain (TMV-c) Tobacco rattle virus (TRV) Tobacco ringspot virus (TRSV) Tobacco streak virus (TSV) Tomato aspermy virus (TAV) Tomato mosaic virus (ToMV) Tomato ringspot virus (ToRSV) Tomato spotted wilt virus (TSWV) Diagnostic Specificity True Negatives 1,714 Correct Diagnoses 1,707 Percent 99.6% Selectivity: No Matrix Effect Observed With: Acalypha leaves Agashache leaves Ageratum leaves Ajuga leaves1 Alternanthera leaves Anagallis leaves Angelonia leaves Antirrhinum leaves Argyranthemum leaves Artemesia leaves Aster leaves Bacopa leaves Begonia leaves Bergenia leaves Bidens leaves Brachycome leaves Bracteantha leaves Brilliantasia leaves Bracteantha leaves Browallia leaves Brugmansia leaves Brunfelsia leaves Calibrachoa leaves Campanula leaves Caryoptersis leaves Chrysanthemum leaves Cleome leaves Coleus leaves2 Colocasia leaves Cosmos leaves Crhysocephal leaves Cuphea leaves Dahlia leaves Dianthus leaves3 Diascia leaves Ensete leaves Euphorbia leaves Euryops leaves Felecia leaves Fern leaves Fuchsia leaves Gaillardia leaves Gaura leaves Gazania leaves Geranium leaves Grebera leaves Gypspholia leaves Hedra Helix leaves Heliopsis leaves Heliotropium leaves Helychrysum leaves Hernaria leaves Heuchera leaves Heucherella leaves Hibiscus leaves Impatiens leaves Ipomoea leaves Lamium leaves Lantana leaves Laurus leaves Lavanda Stoecha leaves Lavander leaves Leucanthemum leaves Lobelia leaves Lysimachia leaves Mercardonia leaves Million Bells leaves Musa leaves Myositis leaves Nemesia leaves New Guinea leaves Nolana leaves Oenothera leaves Osteospermum leaves Otacantus leaves Oxalis leaves Pavonia leaves Pennisetum leaves Penstemon leaves Pentas leaves Pericaria leaves Perilla leaves Perostrophe leaves Petunia leaves Phlox leaves Plectranthus leaves Portulaca leaves Primula leaves Pseuderanthemum leaves Pulmonaria leaves Purslane leaves Quadrifolium leaves Rosa leaves Rosemary leaves Rudbeckia leaves Ruttya leaves Sagina leaves Salvia leaves4 Sanchesia leaves Santolina leaves Sanvitalia leaves Satureja leaves Scabiosa leaves Scaevola leaves Agdia, Inc. 52642 County Road 1 Elkhart, IN 46514 p147.2 574-264-2014 / 800-622-4342 Revised: 09/07/2021 www.agdia.com / [email protected] Page 2 of 3 Scoparia leaves Sedum leaves Senetti leaves Sphaeralcea leaves Spike leaves Stachys leaves Strepcapella leaves Strobilanthes leaves Superbells leaves Supermint leaves Supertunia leaves Surfinia leaves Sutera leaves Thymus leaves Tiarella leaves Torenia leaves5 Tradescantia leaves Tricyrtis leaves Trifolium leaves Turnera leaves Verbascum leaves Verbena leaves Vinca leaves Woegelia leaves 1False positive observed in 1 out of 3 samples of Ajuga. 2False positive observed in 5 out of 80 samples of Coleus. 3False positive observed in 1 out of 3 samples of Dianthus. 4False positive observed in 1 out of 17 samples of Salvia. 5False positive observed in 2 out of 28 samples of Torenia. Agdia, Inc. 52642 County Road 1 Elkhart, IN 46514 p147.2 574-264-2014 / 800-622-4342 Revised: 09/07/2021 www.agdia.com / [email protected] Page 3 of 3.
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