Nicotine Modulates Myd88-Dependent Signaling Pathway in Macrophages During Mycobacterial Infection
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Tessaracoccus Arenae Sp. Nov., Isolated from Sea Sand
TAXONOMIC DESCRIPTION Thongphrom et al., Int J Syst Evol Microbiol 2017;67:2008–2013 DOI 10.1099/ijsem.0.001907 Tessaracoccus arenae sp. nov., isolated from sea sand Chutimon Thongphrom,1 Jong-Hwa Kim,1 Nagamani Bora2,* and Wonyong Kim1,* Abstract A Gram-stain positive, non-spore-forming, non-motile, facultatively anaerobic bacterial strain, designated CAU 1319T, was isolated from sea sand and the strain’s taxonomic position was investigated using a polyphasic approach. Strain CAU 1319T grew optimally at 30 C and at pH 7.5 in the presence of 2 % (w/v) NaCl. Phylogenetic analysis, based on the 16S rRNA gene sequence, revealed that strain CAU 1319T belongs to the genus Tessaracoccus, and is closely related to Tessaracoccus lapidicaptus IPBSL-7T (similarity 97.69 %), Tessaracoccus bendigoensis Ben 106T (similarity 95.64 %) and Tessaracoccus T T flavescens SST-39 (similarity 95.84 %). Strain CAU 1319 had LL-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-9 (H4) as the predominant menaquinone, and anteiso-C15 : 0 as the major fatty acid. The polar lipids consisted of phosphatidylglycerol, phosphatidylinositol, two unidentified aminolipids, three unidentified phospholipids and one unidentified glycolipid. Predominant polyamines were spermine and spermidine. The DNA–DNA hybridization value between strain CAU 1319T and T. lapidicaptus IPBSL-7T was 24 %±0.2. The DNA G+C content of the novel strain was 69.5 mol %. On the basis of phenotypic and chemotaxonomic properties, as well as phylogenetic relatedness, strain CAU 1319Tshould be classified as a novel species of the genus Tessaracoccus, for which the name Tessaracoccus arenae sp. -
Complete Genomic Sequences of Propionibacterium Freudenreichii
UCLA UCLA Previously Published Works Title Complete genomic sequences of Propionibacterium freudenreichii phages from Swiss cheese reveal greater diversity than Cutibacterium (formerly Propionibacterium) acnes phages. Permalink https://escholarship.org/uc/item/7bf0f2q3 Journal BMC microbiology, 18(1) ISSN 1471-2180 Authors Cheng, Lucy Marinelli, Laura J Grosset, Noël et al. Publication Date 2018-03-01 DOI 10.1186/s12866-018-1159-y Peer reviewed eScholarship.org Powered by the California Digital Library University of California Cheng et al. BMC Microbiology (2018) 18:19 https://doi.org/10.1186/s12866-018-1159-y RESEARCH ARTICLE Open Access Complete genomic sequences of Propionibacterium freudenreichii phages from Swiss cheese reveal greater diversity than Cutibacterium (formerly Propionibacterium) acnes phages Lucy Cheng1,2†, Laura J. Marinelli1,2*†, Noël Grosset3, Sorel T. Fitz-Gibbon4, Charles A. Bowman5, Brian Q. Dang5, Daniel A. Russell5, Deborah Jacobs-Sera5, Baochen Shi6, Matteo Pellegrini4, Jeff F. Miller7,2, Michel Gautier3, Graham F. Hatfull5 and Robert L. Modlin1,2 Abstract Background: A remarkable exception to the large genetic diversity often observed for bacteriophages infecting a specific bacterial host was found for the Cutibacterium acnes (formerly Propionibacterium acnes) phages, which are highly homogeneous. Phages infecting the related species, which is also a member of the Propionibacteriaceae family, Propionibacterium freudenreichii, a bacterium used in production of Swiss-type cheeses, have also been described and are common contaminants of the cheese manufacturing process. However, little is known about their genetic composition and diversity. Results: We obtained seven independently isolated bacteriophages that infect P. freudenreichii from Swiss-type cheese samples, and determined their complete genome sequences. -
Alpine Soil Bacterial Community and Environmental Filters Bahar Shahnavaz
Alpine soil bacterial community and environmental filters Bahar Shahnavaz To cite this version: Bahar Shahnavaz. Alpine soil bacterial community and environmental filters. Other [q-bio.OT]. Université Joseph-Fourier - Grenoble I, 2009. English. tel-00515414 HAL Id: tel-00515414 https://tel.archives-ouvertes.fr/tel-00515414 Submitted on 6 Sep 2010 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. THÈSE Pour l’obtention du titre de l'Université Joseph-Fourier - Grenoble 1 École Doctorale : Chimie et Sciences du Vivant Spécialité : Biodiversité, Écologie, Environnement Communautés bactériennes de sols alpins et filtres environnementaux Par Bahar SHAHNAVAZ Soutenue devant jury le 25 Septembre 2009 Composition du jury Dr. Thierry HEULIN Rapporteur Dr. Christian JEANTHON Rapporteur Dr. Sylvie NAZARET Examinateur Dr. Jean MARTIN Examinateur Dr. Yves JOUANNEAU Président du jury Dr. Roberto GEREMIA Directeur de thèse Thèse préparée au sien du Laboratoire d’Ecologie Alpine (LECA, UMR UJF- CNRS 5553) THÈSE Pour l’obtention du titre de Docteur de l’Université de Grenoble École Doctorale : Chimie et Sciences du Vivant Spécialité : Biodiversité, Écologie, Environnement Communautés bactériennes de sols alpins et filtres environnementaux Bahar SHAHNAVAZ Directeur : Roberto GEREMIA Soutenue devant jury le 25 Septembre 2009 Composition du jury Dr. -
Tessaracoccus Massiliensis Sp. Nov., a New Bacterial Species Isolated from the Human Gut
TAXONOGENOMICS: GENOME OF A NEW ORGANISM Tessaracoccus massiliensis sp. nov., a new bacterial species isolated from the human gut E. Seck1, S. I. Traore1, S. Khelaifia1, M. Beye1, C. Michelle1, C. Couderc1, S. Brah2, P.-E. Fournier1, D. Raoult1,3 and G. Dubourg1 1) Aix-Marseille Université, URMITE, UM63, CNRS7278, IRD198, INSERM 1095, Faculté de médecine, Marseille, France, 2) Hôpital National de Niamey, Niamey, Niger and 3) Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia Abstract A new Actinobacterium, designated Tessaracoccus massiliensis type strain SIT-7T (= CSUR P1301 = DSM 29060), have been isolated from a Nigerian child with kwashiorkor. It is a facultative aerobic, Gram positive, rod shaped, non spore-forming, and non motile bacterium. Here, we describe the genomic and phenotypic characteristics of this isolate. Its 3,212,234 bp long genome (1 chromosome, no plasmid) exhibits a G+C content of 67.81% and contains 3,058 protein-coding genes and 49 RNA genes. © 2016 The Author(s). Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases. Keywords: culturomics, genome, human gut, taxono-genomics, Tessaracoccus massiliensis Original Submission: 23 February 2016; Revised Submission: 28 April 2016; Accepted: 3 May 2016 Article published online: 28 May 2016 development of new tools for the sequencing of DNA [5],we Corresponding author: G. Dubourg, Aix-Marseille Université, introduced a new way of describing the novel bacterial species URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Faculté de médecine, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, [6]. This includes, among other features, their genomic [7–11] France and proteomic information obtained by matrix-assisted laser E-mail: [email protected] desorption-ionization time-of-flight (MALDI-TOF-MS) analysis [12]. -
The Microbiota Continuum Along the Female Reproductive Tract and Its Relation to Uterine-Related Diseases
ARTICLE DOI: 10.1038/s41467-017-00901-0 OPEN The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases Chen Chen1,2, Xiaolei Song1,3, Weixia Wei4,5, Huanzi Zhong 1,2,6, Juanjuan Dai4,5, Zhou Lan1, Fei Li1,2,3, Xinlei Yu1,2, Qiang Feng1,7, Zirong Wang1, Hailiang Xie1, Xiaomin Chen1, Chunwei Zeng1, Bo Wen1,2, Liping Zeng4,5, Hui Du4,5, Huiru Tang4,5, Changlu Xu1,8, Yan Xia1,3, Huihua Xia1,2,9, Huanming Yang1,10, Jian Wang1,10, Jun Wang1,11, Lise Madsen 1,6,12, Susanne Brix 13, Karsten Kristiansen1,6, Xun Xu1,2, Junhua Li 1,2,9,14, Ruifang Wu4,5 & Huijue Jia 1,2,9,11 Reports on bacteria detected in maternal fluids during pregnancy are typically associated with adverse consequences, and whether the female reproductive tract harbours distinct microbial communities beyond the vagina has been a matter of debate. Here we systematically sample the microbiota within the female reproductive tract in 110 women of reproductive age, and examine the nature of colonisation by 16S rRNA gene amplicon sequencing and cultivation. We find distinct microbial communities in cervical canal, uterus, fallopian tubes and perito- neal fluid, differing from that of the vagina. The results reflect a microbiota continuum along the female reproductive tract, indicative of a non-sterile environment. We also identify microbial taxa and potential functions that correlate with the menstrual cycle or are over- represented in subjects with adenomyosis or infertility due to endometriosis. The study provides insight into the nature of the vagino-uterine microbiome, and suggests that sur- veying the vaginal or cervical microbiota might be useful for detection of common diseases in the upper reproductive tract. -
Bacteria Isolated from Bengal Cat (Felis Catus
bioRxiv preprint doi: https://doi.org/10.1101/625079; this version posted May 1, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 2 3 4 Bacteria isolated from bengal cat (Felis catus × 5 Prionailurus bengalensis) anal sac secretions produce 6 volatile compounds associated with animal signaling 7 8 9 Mei S. Yamaguchi 1, Holly H. Ganz 2 , Adrienne W. Cho 2, Thant H. Zaw2, Guillaume Jospin2, Mitchell 10 M. McCartney1, Cristina E. Davis1, Jonathan A. Eisen*2, 3, 4, David A. Coil2 11 12 13 14 1 Department of Mechanical and Aerospace Engineering, University of California, Davis, CA, United States 15 2 Genome Center, University of California, Davis, CA, United States 16 3 Department of Evolution and Ecology, University of California, Davis, CA, United States 17 4 Department of Medical Microbiology and Immunology, University of California, Davis, Davis, CA, 18 United States 19 20 21 * Corresponding author 22 Email: [email protected] (JE) 23 24 bioRxiv preprint doi: https://doi.org/10.1101/625079; this version posted May 1, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 25 Abstract 26 Anal sacs are an important odor producing organ found across the mammalian Order Carnivora. -
Downloaded from the NBCI FTP Server As Genbank files and Consisted of Two Strains of P
G C A T T A C G G C A T genes Article A Pan-Genome Guided Metabolic Network Reconstruction of Five Propionibacterium Species Reveals Extensive Metabolic Diversity Tim McCubbin 1, R. Axayacatl Gonzalez-Garcia 1, Robin W. Palfreyman 1 , Chris Stowers 2, Lars K. Nielsen 1 and Esteban Marcellin 1,* 1 Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia; [email protected] (T.M.); [email protected] (R.A.G.-G.); [email protected] (R.W.P.); [email protected] (L.K.N.) 2 Corteva Agriscience, Indianapolis, IN 46268, USA; [email protected] * Correspondence: [email protected] Received: 31 July 2020; Accepted: 10 September 2020; Published: 23 September 2020 Abstract: Propionibacteria have been studied extensively since the early 1930s due to their relevance to industry and importance as human pathogens. Still, their unique metabolism is far from fully understood. This is partly due to their signature high GC content, which has previously hampered the acquisition of quality sequence data, the accurate annotation of the available genomes, and the functional characterization of genes. The recent completion of the genome sequences for several species has led researchers to reassess the taxonomical classification of the genus Propionibacterium, which has been divided into several new genres. Such data also enable a comparative genomic approach to annotation and provide a new opportunity to revisit our understanding of their metabolism. Using pan-genome analysis combined with the reconstruction of the first high-quality Propionibacterium genome-scale metabolic model and a pan-metabolic model of current and former members of the genus Propionibacterium, we demonstrate that despite sharing unique metabolic traits, these organisms have an unexpected diversity in central carbon metabolism and a hidden layer of metabolic complexity. -
INVESTIGATING the ACTINOMYCETE DIVERSITY INSIDE the HINDGUT of an INDIGENOUS TERMITE, Microhodotermes Viator
INVESTIGATING THE ACTINOMYCETE DIVERSITY INSIDE THE HINDGUT OF AN INDIGENOUS TERMITE, Microhodotermes viator by Jeffrey Rohland Thesis presented for the degree of Doctor of Philosophy in the Department of Molecular and Cell Biology, Faculty of Science, University of Cape Town, South Africa. April 2010 ACKNOWLEDGEMENTS Firstly and most importantly, I would like to thank my supervisor, Dr Paul Meyers. I have been in his lab since my Honours year, and he has always been a constant source of guidance, help and encouragement during all my years at UCT. His serious discussion of project related matters and also his lighter side and sense of humour have made the work that I have done a growing and learning experience, but also one that has been really enjoyable. I look up to him as a role model and mentor and acknowledge his contribution to making me the best possible researcher that I can be. Thank-you to all the members of Lab 202, past and present (especially to Gareth Everest – who was with me from the start), for all their help and advice and for making the lab a home away from home and generally a great place to work. I would also like to thank Di James and Bruna Galvão for all their help with the vast quantities of sequencing done during this project, and Dr Bronwyn Kirby for her help with the statistical analyses. Also, I must acknowledge Miranda Waldron and Mohammed Jaffer of the Electron Microsope Unit at the University of Cape Town for their help with scanning electron microscopy and transmission electron microscopy related matters, respectively. -
APUTS) Reporting Terminology and Codes Microbiology (V1.0
AUSTRALIAN PATHOLOGY UNITS AND TERMINOLOGY (APUTS) Reporting Terminology and Codes Microbiology (v1.0) 1 12/02/2013 APUTS Report Information Model - Urine Microbiology Page 1 of 1 Specimen Type Specimen Macro Time Glucose Bilirubin Ketones Specific Gravity pH Chemistry Protein Urobilinogen Nitrites Haemoglobin Leucocyte Esterases White blood cell count Red blood cells Cells Epithelial cells Bacteria Microscopy Parasites Microorganisms Yeasts Casts Crystals Other elements Antibacterial Activity No growth Mixed growth Urine MCS No significant growth Klebsiella sp. Bacteria ESBL Klebsiella pneumoniae Identification Virus Fungi Growth of >10^8 org/L 10^7 to 10^8 organism/L of mixed Range or number Colony Count growth of 3 organisms 19090-0 Culture Organism 1 630-4 LOINC >10^8 organisms/L LOINC Significant growth e.g. Ampicillin 18864-9 LOINC Antibiotics Susceptibility Method Released/suppressed None Organism 2 Organism 3 Organism 4 None Consistent with UTI Probable contamination Growth unlikely to be significant Comment Please submit a repeat specimen for testing if clinically indicated Catheter comments Sterile pyuria Notification to infection control and public health departments PUTS Urine Microbiology Information Model v1.mmap - 12/02/2013 - Mindjet 12/02/2013 APUTS Report Terminology and Codes - Microbiology - Urine Page 1 of 3 RCPA Pathology Units and Terminology Standardisation Project - Terminology for Reporting Pathology: Microbiology : Urine Microbiology Report v1 LOINC LOINC LOINC LOINC LOINC LOINC LOINC Urine Microbiology Report -
Propionibacterium Acnes
Host modulating properties of Propionibacterium acnes Inaugural – Dissertation to obtain the academic degree Doctor rerum naturalium (Dr. rer. nat.) Submitted to the Department of Biology, Chemistry and Pharmacy of Freie Universität Berlin Tim Nam, Mak from Kuala Lumpur, Malaysia 2012 千里之行,始于足下 - 老子 - A journey of a thousand miles begins with the first step - Laozi - 2 This thesis is based on research conducted from 2008 to 2012 at the Max Planck Institute for Infection Biology in Berlin, Germany, under the supervision of Prof. Dr. Thomas F Meyer and Prof. Dr. Holger Brüggemann Reviewers First Reviewer: Prof. Dr. Rupert Mutzel Institute für Biologie Freie Universität Berlin Second Reviewer: Prof. Dr. Thomas F Meyer Max Planck Institute for Infection Biology, Chariteplatz 1, Berlin Date of Defense: 15th July 2012 3 Declaration I hereby declare that the work presented in this thesis has been conducted independently and without any inappropriate support, and that all sources of content, experimental or intellectual, are suitably referenced and acknowledged. I further declare that this thesis has not been submitted before, either in the same or a different form, to this or any other university for a degree. Tim Nam, Mak Berlin, 14 February 2012 4 Acknowledgement First and foremost, I would like to thank Professor Thomas F. Meyer for providing me support, critical discussion to my project and the opportunity to work at this great laboratory. I am also grateful to Professor Rupert Mutzel who gave me good advices and being part of my thesis committee. My special thanks to my direct supervisor Holger Brüggemann. Thank you very much for your patience to guide me step by step from the beginning of my PhD. -
( 12 ) United States Patent
US010398154B2 (12 ) United States Patent ( 10 ) Patent No. : US 10 , 398 , 154 B2 Embree et al. ( 45 ) Date of Patent: * Sep . 3 , 2019 (54 ) MICROBIAL COMPOSITIONS AND ( 58 ) Field of Classification Search METHODS OF USE FOR IMPROVING MILK None PRODUCTION See application file for complete search history . (71 ) Applicant: ASCUS BIOSCIENCES , INC ., San Diego , CA (US ) (56 ) References Cited ( 72 ) Inventors: Mallory Embree, San Diego , CA (US ) ; U . S . PATENT DOCUMENTS Luke Picking , San Diego , CA ( US ) ; 3 , 484 , 243 A 12 / 1969 Anderson et al . Grant Gogul , Cardiff , CA (US ) ; Janna 4 ,559 , 298 A 12 / 1985 Fahy Tarasova , San Diego , CA (US ) (Continued ) (73 ) Assignee : Ascus Biosciences , Inc. , San Diego , FOREIGN PATENT DOCUMENTS CA (US ) CN 104814278 A 8 / 2015 ( * ) Notice : Subject to any disclaimer , the term of this EP 0553444 B1 3 / 1998 patent is extended or adjusted under 35 U .S . C . 154 (b ) by 0 days. (Continued ) This patent is subject to a terminal dis OTHER PUBLICATIONS claimer . Borling , J , Master' s thesis, 2010 . * (21 ) Appl. No .: 16 / 029, 398 ( Continued ) ( 22 ) Filed : Jul. 6 , 2018 Primary Examiner - David W Berke- Schlessel (65 ) Prior Publication Data ( 74 ) Attorney , Agent, or Firm — Cooley LLP US 2018 /0325966 A1 Nov . 15 , 2018 ( 57 ) ABSTRACT Related U . S . Application Data The disclosure relates to isolated microorganisms — includ (63 ) Continuation of application No. ing novel strains of the microorganisms - microbial consor PCT/ US2014 /012573 , filed on Jan . 6 , 2017 . tia , and compositions comprising the same . Furthermore , the disclosure teaches methods of utilizing the described micro (Continued ) organisms, microbial consortia , and compositions compris (51 ) Int. -
Assessment of the Microbiota in Microdissected Tissues of Crohn's
Hindawi Publishing Corporation International Journal of Inflammation Volume 2012, Article ID 505674, 11 pages doi:10.1155/2012/505674 Research Article Assessment of the Microbiota in Microdissected Tissues of Crohn’s Disease Patients Gert De Hertogh,1 Bart Lemmens,1 Peter Verhasselt,2 Ronald de Hoogt,2 Xavier Sagaert,1 Marie Joossens,3 Gert Van Assche,3 Paul Rutgeerts,3 Severine Vermeire,3 and Jeroen Aerssens2 1 Department of Morphology and Molecular Pathology, University Hospitals KULeuven, Minderbroedersstraat 12, 3000 Leuven, Belgium 2 Department Research Informatics & Integrated Genomics, Janssen Pharmaceutical Companies of Johnson & Johnson, Turnhoutseweg 30, 2340 Beerse, Belgium 3 Department of Gastroenterology, University Hospitals KULeuven, Herestraat 49, 3000 Leuven, Belgium Correspondence should be addressed to Gert De Hertogh, [email protected] Received 18 July 2011; Revised 5 September 2011; Accepted 14 September 2011 Academic Editor: Derek Jewell Copyright © 2012 Gert De Hertogh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The microbiota of the gastrointestinal tract is frequently mentioned as one of the key players in the etiopathogenesis of Crohn’s disease (CD). Four hypotheses have been suggested: the single, still unknown bacterial pathogen, an abnormal overall composition of the bowel microbiota (“dysbiosis”), an abnormal immunological reaction to an essentially normally composed microbiota, and increased bacterial translocation. We propose that laser capture microdissection of selected microscopic structures, followed by broad-range 16S rRNA gene sequencing, is an excellent method to assess spatiotemporal alterations in the composition of the bowel microbiota in CD.