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CONTRIBUTION TO THE IDENTIFICATION OF Pseudocercospora SPECIES IN IRAN

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34 < =) 2 %7>=? "=; $ "@ A %7# 8 79( :;( +# #$& !"! Pseudocercospora 6 !" "5( O=<) KMMN? > L ( $ JI#2 +2@ $' K#+2 HI G"C( $ $""( !"! E # & 2FG . BC!# 2 ) P. cruenta , ( Solanum nigrum 2 ) Pseudocercospora atromarginalis = =!" . =7P& #&I + $"( < ! "@ Diospyros lotus 2 ) P. kaki , ( Rubus sp. 2 ) P. heteromalla , ( Phaseolus vulgaris 2 ) P. griseola , ( Vigna sinensis = ( Vitis sylvestris 2 ) P. vitis 2 ( Salix alba 2 ) P. salicina , ( Punica granatum 2 ) P. punicae , ( D. kaki Phaeoisariopsis B=! E=4 T= . !"= =( S#< %#& # +# EA2# #& P. salicina 2 P. heteromalla , !" E # E +# . ! . . /# (V %#& # HI /&'P $ !" & B! . /P &U? Pseudocercospora griseola griseola

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U[@ . ?U N ( _# ? ?Q 2/ H%6 H_i# = ( M( (Hedjaroude 1976) 2U/ HU%6 8 2 ?_ (5J6 TSU 2U/ RS 8 Pseudocercospora Speg. P4Q UN ( U345 Jd 2 : ?D# TS 2/ RS !VUUE<# WUUD 8 P4UUQ XUU . (UU# ,UU5 HUU1 P4UUUQ 8 HUUU%6 ?UUU4^ KUUU JUUU6 XUUU HUUUN 2 8 /"5 Cercospora P4Q 1 (345 E Pseudocercospora U5 ` U5 . U3 :?U#9 J' U \U 2 :%ULS Z,[# 2/% % ?'4N 2 HN 2%Y H1 (Scharif & Ershad 1966) ?UQ 2U/ RS 8 (3L (; ]O5 (8%3 (S,^ 2/ K% ?'4N (% 8 J"3 XU HN ? N H = `-E# 2/ =1J'# 8 :?5 `'Ua% bU31 . U3 ?U_# 2U/ ?U41 1 (% 8 J"3 Pseudocercospora J' P4Q 8 H%6 ?4^ K 3L X !c (Crous & Braun 2003) =1 bN KU = 2/ RS ? ?Q 3L X'4^ e/ . %Q 2 K% ?UU'4N ?UU'% ]UUD# = %UU1 TE

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eN# ' p eN# 2 / H3 c4# /% % .4N'? H U' L ?a v s ' Nu ? ' 3 (%O3O ' # /2 ? 73 K% UU'? 4N ? UU' % ]UUD# UU#O '# v { ,4 'UU3 $%UUY . ] U' "S 8 RUS `U-E# 2 U/ K?U !VE<# Pn3 . ?5 P. vitis Vitis 2 (S,^| / K% '? 4N TE<# \U U_1 =9 \U #U3 %UQ K?U. %Q K% U'? 4N ? U' % ]UD# #O ' # {v ,4 ' 3 $%Y . ?UU41 %UUQ K?UU. UU %UUQ K% UU'? 4N % % 4NUU'? %UY HU1 UE sU^% N U !%Ua H1 TE<# q U3% 8 K% '? 4N Hd !VE<# K% '? 4N % % 4N'? 2 2 ( UN ( S,^| / K% '? 4N \ ': 3 b% D# U 6 S H_i# %# Olympus BH2 g%O3O #' P. griseola Phaseolus HU1 ]VU# e ' U3 HU% U1 ( U/ 'e U3 U/ K? X 8 H H_3% #3 . . ?5 K[ g%O3O #' } } ' H H_3% #3 . Nerium 2 #O'# { X'1 #3 iS . YN 2 C 7! P. neriella HU%6 : J5 H1 w%1# !.7Y x%,[# F ' CD X v v #O '# 8 k-p #3 iS . X ' U1 8 . U3 :?U5 H Pseudocercospora P4Q 8 2 U#O'# ~v { X'U1 $%UY 1 ?4-1 / K% ?'4N . v P. heteromalla = %U4. UD HU%6 U/ H%6 X P. danaicola Danae HN ?451 (# ? ?Q = 2% 1%O' '# 21 P. salicina ~ #O'# } 8 ,N $%Y 1 :%N / K% ?'4N . v . U3 :?5 H / H%6 X %V ]#N ` ' a% H# 2 XUUU5 (UUU-'d (%UUU 8 J"UUU3 UUU/ K% ?UUU'4N . ~ 8 A ' HN Phaseolus vulgaris 2 :?5 H_i# H%, P. paraguayensis Eucalyptus JU6 = 8 Phaeoisariopsis griseola K 1 X 2 2 (%UU 8 J"UU3 HUU1 ] UU ,# 2 :%UULS UU/ K% .4NUU'? ~ Pseudocercospora griseola ? U ?Q KU U1 %1 :?5 P. sphaerellae-eugeniae Eugenia = U 2 U/ RUS 3L / H%6 3 . %5 ( # ( _# r r ?_# 2/?41 2 /% % .4N'? } UL9 8 ( ', U3 U[4 X 141 ? :?#9 ( ) 5 ?41 { 2 !? H1 ?41 ?S /% % .4N'? } . ?U :?U5 UNy :?U5 ( U31 /2 H%, qC :?< H Zizyphus 2 k_<4#'p c4# /% % ?'4N . r 2 2 U1 =6?U; T' E

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? ?41 }{ ?4 / :?',d (N? 3 (S,^| P. atromarginalis Solanum 2 (S,^| U_1 U3 JU' H,' 6 K% ?'4N % E :?.S UL9 $%UY ?4 9 (# ='1 H8 F Y 8 /% % ?'4N . K%U-'/ ?U5 2U'6 :8?U #O'# r{ × { K% ?'4N 2 (8%UUU3 UUU/ K% ?UUU'4N UUU#O'# } { X'UUU1 (. ]OU5 ) ?U51 (U, e'EUf U# U3 \ :'3 (N? P. abelmoschi Hibiscus = UU- 6 =UU3 Rubus sp. 2 : :?UU5 (UU31 HUU%, UU ( %UU8 UUL k_ Pseudocercospora heteromalla Vigna 2 eN# o" % % ?'4N 2/ H3 . XU 8 A'U . %U5 (U# JU6 = 8 1 X' 21 P. cruenta KUUU ( ) UUU5 =UUU 2UUU/ RUUUS UUU3L v eN# 'p % % '? 4N /2 H3 . Rubus sp. Pseudocercospora rubi . 3 :?#9 2 { × v{ kU' H 1 K% ?'4N % % ?'4N _1 . v 1 ?41 =?1 :%N 2/% % ?'4N X5 1 'd H%6 Diospyros 2 #O'# { × {v/ P. heteromalla . %U5 (# J ,# H%6 8 :?4N 2/?41 P. kaki !Uc eU/ U1 J' #3 ]'O< ]D# WD 8 X'4^ e/ v v{ × { kU' H1 K% ?'4N % % ?'4N _1 . v #U3 P. heteromalla HU%6 HN 2%Y H1 ? Punica 2 #O'# }~{ × {v/ P. punicae li3 #3 P. rubi H%6 / t1 (D li3 Pseudocercospora heteromalla (Syd.) Deighton, o?U,. \U :'U3 U/ HO %5. (# ]'O< / t1 ( Trans. Brit. Mycol. Soc. 88: 388. (1987)

( v/ /v{) HO . ?4 / ?# H,' (/6 e4# ]U ,# 2 :%LS H'5@ ?4 / ?# H,' ?# / HO sUU^%N 2UU/ HUU3 /% % ?UU'4N UU3 UU# (UU-'# (/U6 ?451 (# 2 :%LS H1 ] ,# 8 JN# J#S H1 (U liU3 ?4 U/ HU v{ 8 ]O

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[&=== 2 T=== FG (C) === B" === (B) "P" === (A) Rubus sp. 2 Pseudocercospora heteromalla . ZO=== .( .( &7(2&O( ]^ = \M( ) Fig. 1. Pseudocercospora heteromalla on Rubus sp. A. Conidiophores, B. Conidia C. Symptoms on leaf (Bar = 50 µm).

HUU%6 !VUUE<# UU1 :?UU5 (UU31 HUU%, !VUUE<# U/ K% ?U'4N U3 TE<# K% ?'4N ?'% ]D# ?4N (# qUU3% :?UU5 `'UUa% Pseudocercospora salicina (N?U U U3 2 H%U3 (S,^| hc5 c4# % % ?U'4N $%UY ?U4^/ UC1i# ( }~ ) =% ?U ?41 { ?451 (# ha 8 :% 1 :?',d 8 1 X' 21 3 <'1 (N? H%, X K% ?'4N %U5 (# :? (; ?41 ]D# / K% ?'4N (d1 %5. %5. (# J6 = U_1 U3 U6 kU-p K% ?U'4N % E :?.S Hibiscus cannabinus L. 2 : :?5 (31 2/ H%, ?UU5 2UU'6 :8?UU UU#O'# }v{ × v/ v{ K% ?UU'4N TE

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[&=== 2 T === FG (C) === B" === (B) "P" === (A) Salix alba 2 Pseudocercospora salicina . ZO=== .( .( &7(2&O ( ]^ = \ )M( Fig. 2. Pseudocercospora salicina on Salix alba . A. Conidiophores, B. Conidia C. Symptoms on leaf (Bar = 50 µm).

Vigna (L.) Endl. 2 : :?UU5 (UU31 2UU/ HUU%, . ' \ :'3 e'Ef K% ?'4N :?.S r H/v/ ' UU5 HUU39 =7 ' UU6 =UU3 sinensis Pseudocercospora atromarginalis (G.F. Atk.) Deighton, Mycol. Pap. 140: 139. (1976) =7 'UU6 =UU3 (IRAN 15301 F) 'UU' 29 ZUU,Q Solanum nigrum L. 2 : :?U5 ( U31 2 U/ HU %, (IRAN 15302 '' 29 Z,Q r /v/ 3 H_#%a (U 8 ' # 29 ZU,Q r r// =U[ ' /u =7 ' U6 =3 TE

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Cercospora jujubae S. Chowdhury KU %NU# rr r/r/ UUU3 H_#%UUUa =7 'UUU6 =UUU3 vulgaris K ( ) =1 bN 3L # ? : N ( _# . (IRAN 15297 F) 3?d 29 Z,Q UU3 KUU =%UU4. HUU1 Pseudocercospora jujubae U' 1% 2 U/ tU1 2 :?5 [ e 7. H1 HQ% 1 K% U'? 4N ? U' % ]UD# H U 9 X U . U3 :?U5 H U' 1% tU1 U H2 8 HUO =%U4. UD 2, 1 X' Z14# K%U# UL ?4-1 k-p /% % '? 4N 3 TE<# RUUUUS =9 :?UUUU44N UUUU[ ]UUUU#. 2UUUU;# % % '? 4N . X 4^' e/ . ?4 / ?Q%# ( %8 6 = . 3 :?5 ( _# Phaeoisariopsis griseola P4UQ 2 U/ HU%6 U3 8 < U' 1 HU%6 X K% '? 4N UU3 :?UU5 JUU6 UU' 1% 2 8 H UU 9 X 'UU,/ J UU' . . 3 Pseudocercospora (Crous et al. 2006) =O,/ bN . (Ershad 2009) Pseudocercospora kaki Goh & W.H. Hsieh, in RS s ' #%% N ' _f X '' _ %4# H1 F ' CD s Hsieh & Goh, Cercospora and similar fungi Phaeoisariopsis griseola from Taiwan: 109. (1990) 2 /? U' %&-N% ( 1( % 1 =3 Diospyros kaki L. 2 ::?5 (31 /2 H%, (SSU rDNA) ( #8%" DNA s^%N ?@ 8 H '@ x8 'UU' 29 ZUU,Q r /r/ ?UU4 = UU-6 WUUD 8 Phaeoisariopsis P4UUQ HUUN ?UU =

v : / / / x8 U' ' 29 Z,Q r /r/ 2 %N N H1 =66 :?5 J6 (Petrak & Esfandiari 1941) 2 ?4c3 r~ // X#?U5 =7 '6 =3 (IRAN 15314 F) HU%6 'X U,/ J ' (Khabiri 1958) 2 "d' Pn3 . 3 =?U8# =U3 (IRAN 15315 F) 2 ?/8 29 Z,Q w%U1# U/ J6 3 . 3 : N ( _# D. kaki 2 UUU5 uUUU6 29 ZUUU,Q } ~/}/ % UUUL5 UU5 ` UU5 (Esfandiari 1946) 2 ?4cUU3 HUU1 v ~/v/ LU5% =?U8# =3 (IRAN 476 F) K HN ?51 (#( } ) =O,/ =|%1 %4{ ( ~~ ) =7 '6 =3 (IRAN 477 F) J 5 29 Z,Q ( UU _# Diospyros lotus 2 Cercospora kaki AU 29 ZU,Q ~ ~/}/ U4O19 ]U;4Q ( J ?41 ( U31 1 (Hsieh & Goh 1990) :%6 H /' . ? : N %US ]U# L

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r /~/ ]U1 1 =?U8# =3 (IRAN 15316 F) sUU3Q UUL1 :UU^ X 'UU1 = UU^%-1 = UU' 3 =UU3 =UUU3 (IRAN 15317 F) (UUUQS9 29 ZUUU,Q =U3 (IRAN 483 F) U5 29 ZU,Q vv v/r/ (Dc%U1 U'# 29 ZU,Q ~r /}/ LU5% =?8# v~ // sU3Q U4 # X' ' 1 = ^%-1 = 3' =UU3 Vitis vinifera L. 2 .(IRAN 7129 F) . (IRAN 484 F) 5 29 Z,Q 2 2 ?UU,D# 29 ZUU,Q r // b%UU^ =?UU8# (Ershad 1990) 5 q3% = J6 X ' ~ /v/ UU5 =7 'UU6 =UU3 (IRAN 11696 F) KUUU %NUUU# =UUU1J '# 2 =9 HUUUN ?UUU5 Pseudocercospora vitis KU ( }~ ) [@ . 3 :?5 Pseudocercospora sphaerellae-eugeniae (Sacc.) Crous, Alfenas & R.W. Barreto, 2 2 U/ HU%, K% U'? 4N !VUE<# . UN ( _# = 8 Mycotaxon 64: 425. (1997) U[@ qU3% HUN HU9 U1 F ' CD X :?5 (31 Eugenia jambos L. 2 : :?U5 ( U31 2 U/ HU%, !Uc ( N?U 3 :?5 e ' 3 ` 'a% ( }~ ) v / P/ ' U UL1 :^ = ^%-1 = ' 3 =3 ( ( U; ?U41 ]UD# U/ K% '? 4N ( d1 HN 2 %Y H1 = UU' 3 =UU3 (IRAN 464 F) UU5 29 ZUU,Q ( ( %U 8 J"U3 U XU5 2 :%LS L9 \ %5 ( # :? U5 29 Z,Q vv /r/ P ' L1 :^ = ^%-1 U/ K% U'? 4N \U ( }~ ) U[@ HUN ( @ 3 . (IRAN 465 F) ]UD# ( U; %UQ HU1 : UN Ny : ' 2 :%LS ?U5 <4# ( ) 5 q3% = J6 X' . . 3 : O :5 K% '? 4N 2/?41 Cercospora eugeniae (Rangel) Chupp KU =9 HN H 9 K X ( .?S , 3 :?5 ( _# %N# =1J ' # 2 #<_` U# ?51 (# Cercosporina sphaerellae-eugeniae b?UU4L# (UUQS9 (UU-. ?UU,D# UUN =UU S9 8 =6?UU; H1 H 9 K K% '? 4N ? ' % ]D# = %1 TE<# H1 HQ% 1 (U 8'# Uf ?U,D# b?4L# ("Lia 2 %Q f'-. UU'' > Pseudocercospora sphaerellae-eugeniae ( ?US U/ HU%, 8 2 ?U_ = US U' d ]U' H1 . (Crous et al . 1997) Pseudocercospora vitis (Lev.) Speg., Annales . . ?4 , (# Mus. Nac. Hist. Nat. Buenos Aires 20: 438. (1910)

J( Vitis sylvestris Gmelin 2 : :?U5 (31 /2 H%, . %5 H_Q# ( '-; X# ( 111 -112 ) !Dca H1 H@7# LQ U' ' 29 ZU,Q r /v/ U3 H_#%Ua =7 ' 6 =3

} Iran. J. Plant Path., Vol. 48, No. 3, 2012: 111 -112

CONTRIBUTION TO THE IDENTIFICATION OF Pseudocercospora SPECIES IN IRAN

M. PIRNIA 1*, R. ZARE 2, H. R. ZAMANIZADEH 3, A. KHODAPARAST 4 and B. DJAVADI 2

(Received: 2. 7. 2011; Accepted: 18. 4. 2012)

Abstract Eight collections of Pseudocercospora species from different localities in Northern provinces of Iran were obtained and examined during Summer-Autumn of 2010. Furthermore all of specimens belonging to the genus Pseudocercospora deposited in fungal collection of Ministry of Jihad-e- Agriculture in Iranian Research Institute of Plant Protection were re-examined. Eight species on nine host plants were identified including: Pseudocercospora atromarginalis (on Solanum nigrum ), P. cruenta (on Vigna sinensis ), P. griseola (on Phaseolus vulgaris ), P. heteromalla (on Rubus sp.), P. kaki (on Diospyros lotus , D. kaki ), P. punicae (on Punica granatum ), P. salicina (on Salix alba ), P. vitis (on Vitis sylvestris ). Among these, P. heteromalla and P. salicina are new records for mycobiota of Iran. The name of Phaeoisariopsis griseola changed into Pseudocercospora griseola .Other species were already been reported from Iran.

Keywords: Pseudocercospora , New species, Host plant, Leaf spot.

See Persian text for figures and tables (Pages ).

*: Corresponding Author, Email: [email protected] 1. Assis. Prof. of Plant Pathol., Islamic Azad University, Damghan Branch, Damghan, Iran. 2. Res. Prof. and Researcher of Iranian Research Institute of Plant Protection, Tehran, Iran. 3. Assoc. Prof. of Plant Pathol., Islamic Azad University, Science and Research Branch, Tehran, Iran. 4. Assoc. Prof. of Plant Pathol., College of Agriculture, University of Guilan, Rasht, Iran.

111 Pirnia et al .: Contribution to the identification of Pseudocercospora species in iran

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