Test Code Test Name Components Specimen Method Comments Report *CD3 *CD19 2 mL (1 mL min.) serum from 1 SST AND 6 mL (4 mL *CD16 + min.) whole blood in 2 Lavender Top (EDTA) tubes ABORTION CD56*TNF-alpha AND 3 mL (2 mL min.) whole blood in 1 Green Top Flow Cytometry, Sample by Mon 4 Z785 PANEL *HLA DR, DQA1 (Sodium Heparin) tube. Ship immediately at CLIA, PCR pm; Report Wed & DQB1 (Class II) 18–22°C. DO NOT REFRIGERATE OR FREEZE. Specify Typing time, date and clinical details on test request form. ABSOLUTE 3 mL (1 mL min.) whole blood in 1 Lavender Top Electrical H022 EOSINOPHIL Daily (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Impedance, VCS COUNT; AEC ABSOLUTE 3 mL (1 mL min.) whole blood in 1 Lavender Top Electrical H052 LYMPHOCYTE Daily (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Impedance, VCS COUNT ABSOLUTE 3 mL (1 mL min.) whole blood in 1 Lavender Top Electrical H051 NEUTROPHIL Daily (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Impedance, VCS COUNT ACETYLCHOLINE Sample by 7th of RECEPTOR 2 mL (0.5 mL min.) serum from 1 Red Top (No the month; RW002 (ACHR) Additive) tube. Ship refrigerated or frozen. Report after 2–3 BLOCKING weeks This assay should not be requested for patients who have ACETYLCHOLINE recently received RECEPTOR 2 mL (1 mL min.) serum from 1 SST. Ship radio-isotopes Sample by Wed / S220 (ACHR) refrigerated or frozen. Avoid general anaesthetic or Radioimunoassay therapeutically or Sat 9 am; Report BINDING muscle relaxant drugs 24 hours prior to sampling. diagnostically due to Same day ANTIBODY potential assay interference. Also see MYASTHENIA GRAVIS PANEL.

PAGE 1 OF 399 ACETYLCHOLINE 2 mL (0.5 mL min.) serum from 1 Red Top (No Sample by 7th of RECEPTOR Additive) tube. Do not use SST gel barrier tubes. the month; RW003 (ACHR) Ship refrigerated or frozen. Avoid general Radiobinding Assay Report after 2–3 MODULATING anaesthetic or muscle relaxant drugs 24 hours prior weeks ANTIBODY to sampling. 3 ml (1 mL min.) amniotic fluid in a sterile screw ACETYLCHOLINE Sample by 7th of capped vial. Ship refrigerated or frozen. Indicate Sample should be STERASE, the month; RW004 gestational age, collection date, clinical indications Gel Electrophoresis drawn between 14- AMNIOTIC Report after 2–3 and the AFP and MoM results if available on the 21 weeks gestation FLUID weeks Test Request Form. *Acid 2 mL (0.5 mL min) serum from 1 SST. Keep whole ACID Phosphatase specimen refrigerated until serum can be separated. B054 PHOSPHATASE, Total*Acid Spectrophotometry Daily Separate serum, add 2 drops (60 uL) 5% Acetic acid PROSTATIC Phosphatase and mix. Ship refrigerated or frozen. Prostatic 2 mL (0.5 mL min.) serum from 1 SST. Keep whole ACID specimen refrigerated until serum can be separated. B053 PHOSPHATASE, Spectrophotometry Daily Separate serum, add 2 drops (60 uL) 5% Acetic acid TOTAL and mix. Ship refrigerated or frozen. 2 mL (0.5 mL min.) plasma from 1 Lavender Top (EDTA) tube. Draw blood between 8–10 a.m. Mix Sample Daily by 4 Chemiluminescent R040 ACTH well, separate and transfer plasma IMMEDIATELY pm; Report Same Immunoassay into a sterile screw capped vial and FREEZE. Ship day frozen. DO NOT THAW.

PAGE 2 OF 399 It is recommended 3 mL Whole blood in 1 Blue Top (Sodium Citrate) that patient tube. Mix thoroughly by inversion. Transport to Lab discontinues within 4 hours. If this is not possible, make PPP Heparin for 1 day within 1 hour of collection as follows: Centrifuge and Oral ACTIVATED sample at 3600 rpm for 15 min & transfer Anticoagulants for 7 Sample by PROTEIN C - supernatent to a clean plastic tube. Centrifuge this Electromechanical days prior to 2nd/4th Sat by 9 E016 RESISTANCE; supernatant again at 3600 rpm for 15 mins & finally Clot Detection sampling as these am; Report Next APCR trasfer the supernatant (PPP) to 1 labelled, clean drugs may affect Mon plastic screw capped vial. FREEZE IMMEDIATELY. test results. Ship frozen. DO NOT THAW. Overnight fasting is Discontinuation preferred. Duly filled Coagulation Requisition Form should be with prior is mandatory. consent from the treating Physician

ACTN3 (SPORTS Sample by Thu 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS100 GENE), PCR Sequencing 11 am; Report (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. GENOTYPING 10 days

PAGE 3 OF 399 *t (8;21) or LSI ETO/AML1 * inv (16) (p13q22) or t(16;16) (p13;q22) or ACUTE CBFB * t (15;17) MYELOID 8 mL (6 mL min.) whole blood from 2 Green Top or LSI PML/RARA Sample Daily by 6 LEUKEMIA (Sodium Heparin ) tubes AND 4 mL (2 mL min.) FISH & Conventional Z774 * pm; Report 7 (AML), Bone Marrow from 1 Green Top (Sodium Heparin) Karyotyping t(variable;11q23) Days CYTOGENETICS tube. Ship at 18-22°C. DO NOT FREEZE. ; MLL gene PANEL breakapart *Chromosome analysis, Hematological malignancy

*Cystatin C *Neutrophil Gelatinase - Associated CMIA, 5 mL ( 3 mL min.) serum from 1 SST AND 10 mL (5 Sample by Mon / ACUTE KIDNEY Lipocalin (NGAL) , Z805 mL min.) aliquot of random urine in a sterile screw Wed / Fri 4 pm; INJURY PANEL *Creatinine, Jaffe's reaction, capped container. Ship refrigerated or frozen. Report Same Day serum Indirect ISE *Fractional excretion of Sodium

PAGE 4 OF 399 *BCR/ABL or Philadelphia translocation *t(variable;11q2 ACUTE 3) ; MLL gene LYMPHOBLASTI 8 mL (6 mL min.) whole blood from 2 Green Top breakapart Sample Daily by 6 C LEUKEMIA (Sodium Heparin ) tubes AND 4 mL (2 mL min.) FISH & Conventional Z775 * t (12;21) pm; Report 7 (ALL), Bone Marrow from 1 Green Top (Sodium Heparin) Karyotyping (p13;q22); Days CYTOGENETICS tube. Ship at 18-22°C. DO NOT FREEZE. TEL/AML1 PANEL *Chromosome analysis, Hematological malignancy 2 mL (1 mL min.) serum from 1 SST OR 2 mL (1 mL ADENOSINE min.) plasma from 1 Lavender Top (EDTA) tube OR 2 R156 DEAMINASE ; mL (1 mL min.) Body Fluid / CSF in a sterile screw Spectrophotometry Daily ADA capped container. Ship refrigerated or frozen. Hemolysed samples are not acceptable. ADENOVIRUS Sample Daily by 1 ANTIGEN FOR 5 g fresh STOOL in a sterile screw capped container. M211 ICT pmReport Same GASTROENTERI Ship refrigerated only. DO NOT FREEZE. day TIS. STOOL ADENOVIRUS, 3 ml (2 mL min.) whole blood from 1 Lavender Top Sample by N126 QUALITATIVE, (EDTA) tube OR 3 ml (2 mL min.) CSF. Ship Real Time PCR Mon/Thu 11 am; PCR refrigerated. DO NOT FREEZE. Report Wed/Sat ADENOVIRUS Sample Daily by 1 ANTIGEN FOR 5 g fresh STOOL in a sterile screw capped container. M213 ICT pmReport Same RESPIRATORY Ship refrigerated only. DO NOT FREEZE. day INFECTIONS

PAGE 5 OF 399 ADRB2 GENOTYPING Sample by Sat 11 FOR BETA-2 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS102 PCR Sequencing am; Report 10 AGONIST (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. days RESPONSIVENES S

This test can be used in conjunction with other AFB Cultures Submit pure growth of AFB on appropriate media in on specific request screw capped container in sealed plastic bag. Ship as additional test. AFB refrigerated OR when used with other AFB culture For Nucleic Acid Sample Daily by IDENTIFICATION Automated M094 tests, the pure growth obtained in the Laboratory Probes, use test 3.30 pm; Report , RAPID; PNB Fluorescent from specimens already submitted will be code N001; for AFB 15 days TEST processed for Mycobacterium TB Complex / MOTT Antibiotic indentification. Sensitivity, use appropriate test code. Request as additional tests.

Miscellaneous specimens (Urine, Pus, Body Fluids like pleural, peritoneal, pericardial, gastric, bronchial, semen etc.) in a sterile, screw capped container OR Swabs in sterile normal saline OR Bone marrow in 1 Green Top (Sodium Heparin) tube Fluorescent and AFB STAIN, Auramine and For tissue specimens Sample Daily by OR smeared onto 4 clean glass slides fixed conventional P030 MISCELLANEOU Ziehl Neelsen use test code J050 3.30 pm; Report immediately in 95% ethanol for 30 minutes and air microscopic S staining AFB Stain Same day dried. For gastric lavage , patient should be fasting examination for previous 12 hours. Transport the gastric lavage specimens within 4 hrs of collection. Specify type of specimen on container and test request form. Ship refrigerated.

PAGE 6 OF 399 AFP (ALPHA Also see MATERNAL FETOPROTEIN), 3 mL (1 min.) Amniotic fluid. Avoid contamination Chemiluminescent R084 SERUM SCREENING Daily AMNIOTIC with maternal blood. Ship refrigerated or frozen. Immunoassay PANELS. FLUID AFP (ALPHA Recommended for FETOPROTEIN), 2 mL (0.5 mL min.) serum from 1 SST. Ship Chemiluminescent F001 cancer follow up Daily TUMOR refrigerated or frozen. Give brief clinical history. Immunoassay only. MARKER

The test is recommended only AFP (ALPHA between 14–22 2 mL (0.5 mL min.) serum from 1 SST. Ship Chemiluminescent R030 FETOPROTEIN), weeks of pregnancy. Daily refrigerated or frozen. Immunoassay MATERNAL Also see MATERNAL SERUM SCREENING PANELS.

Submit Throat swabs in Aerobic transport medium Gram staining and P094 ALBERT STAIN available from LPL OR 2 air dried smears on sterile Metachromatic Daily glass slides. Ship refrigerated. DO NOT FREEZE. staining Sample Mon 2 mL (1 mL min.) Cerebrospinal fluid (CSF) in a through Sat by 4 B094 ALBUMIN, CSF sterile screw capped container. Ship refrigerated or Nephelometry pm; Report Same frozen. day ALBUMIN, 2 mL (1 mL min.) Body fluid in a sterile screw P086 BCG Daily FLUID capped container. Ship refrigerated or frozen. Sample Mon 2 mL (1 mL min.) Cerebrospinal fluid (CSF) in a ALBUMIN & *CSF IgG/ Also see MULTIPLE through Sat by 4 Z088 sterile screw capped container. Ship refrigerated or Nephelometry IgG, CSF Albumin Ratio SCLEROSIS PANELs. pm; Report Same frozen. day ALBUMIN, 2 mL (0.5 mL min.) serum from 1 SST.Overnight B039 BCG Daily SERUM fasting is preferred. Ship refrigerated or frozen.

PAGE 7 OF 399 ALCOHOL ETHYL GLUCURONIDE Sample Daily by 5 (EtG) & ETHYL 5 mL (2 mL min.) aliquot of random urine without G184 LC-MS/MS pm; Report Next SULPHATE preservative. Ship refrigerated or frozen. day (EtS), QUANTITATIVE ASSAY, URINE

ALCOHOL 3 mL (2 mL min.) serum from 1 SST. Cleanse (ETHANOL) Alcohol K017 collection site with NON-ALCOHOLIC agent before Daily SCREEN, Dehydrogenase UV drawing blood. Ship refrigerated or frozen. SERUM ALDEHYDE 2 mL (0.5 mL min.) serum from 1 SST.Ship S024 CHOPRA TEST Tube clotting Daily refrigerated or frozen. FOR KALA AZAR

2 mL (0.5 mL min.) serum from 1 SST OR 2 mL (0.5 mL min) plasma from 1 Green Top (Sodium Heparin) B052 ALDOLASE Spectrophotometry Daily / Lavender Top (EDTA) tube. Hemolysed specimens are not acceptable. Ship refrigerated or frozen.

A complete investigation of 2 mL (0.5 mL min.) serum from 1 SST. Patient should Aldosteronepathoph Sample by Fri 9 ALDOSTERONE, be ambulatory / upright 2 hours prior to sampling. R049 CLIA ysiology must am; Report Same SERUM Ship refrigerated or frozen. Overnight fasting is include assessment day preferred. of PLASMA RENIN, ACTIVITY.

PAGE 8 OF 399 2 mL (1 mL min.) serum from 1 SST AND 3 mL (2.5 mL min.) plasma from 1 Lavender Top (EDTA) tube. ALDOSTERONE Collect blood between 8.00-10.00 am. Patient Sample by Fri 9 / PLASMA B175 should be ambulatory / upright 2 hours prior to CLIA am; Report Same RENIN DIRECT sampling. Separate plasma in a plastic transport vial day RATIO and freeze. Ship frozen. DO NOT THAW. Overnight fasting is preferred.

A complete 5 mL (1 mL min.) aliquot of 24-hour urine. Keep investigation of refrigerated during collection. Add boric acid ALDOSTERONE, Aldosteronepathoph Sample by Thu 4 (1g/100 ml urine) to the 24-hour collection to U039 24-HOUR CLIA ysiology must pm; Report Next maintain pH below 7.5. Mix well and aliquot. Ship URINE include assessment day refrigerated or frozen. Record 24-hour volume on of PLASMA RENIN, urine container and test request form. ACTIVITY.

Also see NTx ALKALINE 2 mL (1.5 mL min.) serum from 1 SST. Separate (COLLAGEN CROSS- Total alkaline Sample by Thu 9 PHOSPHATASE serum within 1 hour of collection. Ship refrigerated Agarose Gel LINKED N- E011 phosphatase & am; Report Next (ALP) or frozen. Hemolysed specimens are not acceptable. Electrophoresis TELOPEPTIDE);PYRIDI Isoensymes day ISOENZYMES Overnight fasting is preferred. NIUM COLLAGEN CROSS -LINKS Also see NTx (COLLAGEN CROSS- ALKALINE 2 mL (0.5 mL min.) serum from 1 SST. Hemolysed LINKED N- Sample by Tue 9 PHOSPHATASE, Enzyme R121 specimens are not acceptable. Ship refrigerated or TELOPEPTIDE); am; Report Same BONE-SPECIFIC; Immunoassay frozen. PYRIDINIUM day BSAP COLLAGEN CROSS LINKS ALKALINE 2 mL (0.5 mL min.) serum from 1 SST. Hemolysed B041 PHOSPHATASE; specimens are not acceptable. Ship refrigerated or IFCC Daily ALP frozen. Overnight fasting is preferred.

PAGE 9 OF 399 ALKAPTONURIA, 15 mL (10 mL min.) aliquot of random urine in a Gas Sample Daily by 5 Homogentisic G182 URINE, sterile screw capped container without Chromatography/Ma pm; Report 5 Acid QUANTITATIVE preservative. Ship refrigerated or frozen. ss Spectrometry days

10 mL (5 mL min.) aliquot of freshly voided urine in ALKAPTONURIA, a sterile screw capped container. No preservative G102 URINE, required. Wrap container in aluminium foil to Chemical Daily QUALITATIVE protect from light. Ship refrigerated or frozen. Clinical and drug history must accompany sample. Common Ragweed,Dermat ophagoides pteronyssinus, Dermatophagoid ALLERGY es farinae, ASTHMA / Sample Daily by Alterneria 6 mL (4 mL min.) serum from 2 SST's.Ship Z684 RHINITIS ImmunoCAP 11 am; Report 3 tenuis,Aspergillus refrigerated or frozen. SCREENING days fumigatus, PANEL ADULT Candida albicans,Penicilliu m notatum, House dust & Cockroach ALLERGY NON- Fish, Shrimp, Sample Daily by 6 mL (4 mL min.) serum from 2 SST's.Ship Z695 VEGETARIAN Lobster Mussel, ImmunoCAP 11 am; Report 3 refrigerated or frozen. PANEL 2 Tuna & Salmon days ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A038 BUCKWHEAT 11am; Report refrigerated or frozen. IgE (Kutu) Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A077 11am; Report CASEIN refrigerated or frozen. IgE Same Day

PAGE 10 OF 399 ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A030 CHICKEN 11am; Report refrigerated or frozen. IgE (Murgi) Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A007 CLADOSPORIU 11am; Report refrigerated or frozen. IgE M HERBARUM Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A071 11am; Report COCOA refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, COW 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A014 11am; Report DANDER refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, DOG 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A015 11am; Report DANDER refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, ELM 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A090 11am; Report (Papdi Chibil) refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A088 GOOSEFOOT 11am; Report refrigerated or frozen. IgE (Bathua) Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A008 11am; Report HOUSE DUST refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A066 11am; Report LENTIL (Dal) refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A034 11am; Report MELALEUCA refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, MILK 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A006 11am; Report (Doodh) refrigerated or frozen. IgE Same Day

PAGE 11 OF 399 ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A096 MULBERRY 11am; Report refrigerated or frozen. IgE (Shahtoot) Same Day Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A035 ALLERGY, OAT 11am; Report refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A046 11am; Report ONION (Pyaaz) refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, PEA 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A067 11am; Report (Matar) refrigerated or frozen. IgE Same Day ALLERGY,PENICI Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A003 LLIUM 11am; Report refrigerated or frozen. IgE NOTATUM Same Day Spinach, Cabbage, Paprika Tomato, ALLERGY Mushroom, Sample Daily by VEGETABLE 6 mL (4 mL min.) serum from 2 SST's.Ship Z531 Garlic, Onion, ImmunoCAP 11 am; Report 3 COMPREHENSIV refrigerated or frozen. Celery, Carrot, days E PANEL Potato, Cucumber & Brinjal Tomato, ALLERGY Sample Daily by Spinach, 6 mL (4 mL min.) serum from 2 SST's.Ship Z687 VEGETABLE ImmunoCAP 11 am; Report 3 Cabbage & refrigerated or frozen. PANEL 2 days Paprika

PAGE 12 OF 399 Ragweed, Goosefoot, Common Sample Daily by ALLERGY 6 mL (4 mL min.) serum from 2 SST's.Ship Z526 pigweed, ImmunoCAP 11 am; Report 3 WEED PANEL refrigerated or frozen. Cocklebur, days Mugwort & Rapeseed House dust, Mites likeDermatophag Sample Daily by ALLERGY DUST oides 6 mL (4 mL min.) serum from 2 SST's.Ship Z161 ImmunoCAP 11 am; Report 3 PANEL Pteronyssinus, refrigerated or frozen. days Dermatophagoid es Farinae & Cockroach Acacia, Olive, Willow, Sample Daily by ALLERGY TREE 6 mL (4 mL min.) serum from 2 SST's.Ship Z162 Melaleuca, ImmunoCAP 11 am; Report 3 PANEL refrigerated or frozen. White pine & days Eucalyptus Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A093 11 am; Report ALDER (Bhidur) refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A049 11am; Report APPLE (Seb) refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A005 ASPERGILLUS 11am; Report refrigerated or frozen. IgE FUMIGATUS Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A050 11am; Report BANANA (Kela) refrigerated or frozen. IgE Same Day

PAGE 13 OF 399 Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A040 ALLERGY, BEEF 11am; Report refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A069 11am; Report CAROB refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A059 11am; Report CARROT (Gajar) refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A047 CELERY 11am; Report refrigerated or frozen. IgE (Ajmoda) Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A073 CHEDDAR 11am; Report refrigerated or frozen. IgE (Cheese) Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A070 CHICKPEA 11am; Report refrigerated or frozen. IgE (Kabuli chana) Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A010 11am; Report COCKROACH refrigerated or frozen. IgE Same Day ALLERGY, COMMON Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A089 PIGWEED 11am; Report refrigerated or frozen. IgE (Kantewali Same Day Chauli) Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A095 11am; Report COTTONWOOD refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A061 CUCUMBER 11am; Report refrigerated or frozen. IgE (Kheera) Same Day

PAGE 14 OF 399 Sample Daily by ALLERGY, EGG 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A041 11am; Report YOLK (Anda) refrigerated or frozen. IgE Same Day ALLERGY, BERMUDA Sample Daily by GRASS 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A022 11am; Report (Cynodon refrigerated or frozen. IgE Same Day dactylon / Doob) ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A097 COCKLEBUR 11am; Report refrigerated or frozen. IgE (Bichha / Kutta) Same Day Sample Daily by ALLERGY, EGG 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A016 11am; Report WHITE (Anda) refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A045 11am; Report GARLIC (Lasoon) refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A019 11am; Report GLUTEN (Lasa) refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A013 11am; Report HORSE DANDER refrigerated or frozen. IgE Same Day ALLERGY, JOHNSON Sample Daily by GRASS 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A023 11am; Report (Sorghum refrigerated or frozen. IgE Same Day halepense / Jowar) ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A057 LEMON 11am; Report refrigerated or frozen. IgE (Neembu) Same Day

PAGE 15 OF 399 Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A080 11am; Report MANGO (Aam) refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by MEADOW 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A084 11am; Report GRASS (Lawn refrigerated or frozen. IgE Same Day grass) ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A091 MESQUITE 11am; Report refrigerated or frozen. IgE (Pahari Keekar) Same Day

Dermatophagoid Sample Daily by es 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A009 ALLERGY, MITES 11am; Report pteronyssinus, refrigerated or frozen. IgE Same Day Dermatophagoid es farinae ALLERGY, MUG Sample Daily by WORT 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A025 11am; Report (Artemisia refrigerated or frozen. IgE Same Day vulgaris / Barna) ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A079 MUTTON 11am; Report refrigerated or frozen. IgE (Meat) Same Day Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A094 ALLERGY, OAK 11am; Report refrigerated or frozen. IgE Same Day Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A032 ALLERGY, OLIVE 11am; Report refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A048 ORANGE 11am; Report refrigerated or frozen. IgE (Santara) Same Day

PAGE 16 OF 399 Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A054 11am; Report PAPRIKA refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, PEAR 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A056 11am; Report (Nashpati) refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A062 11am; Report PECAN refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A063 CASHEWNUT 11am; Report refrigerated or frozen. IgE (Kaju) Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A076 COCONUT 11am; Report refrigerated or frozen. IgE (Nariyal) Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A027 EUCALYPTUS 11am; Report refrigerated or frozen. IgE (Nilgiri / Safeda) Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A078 11am; Report LOBSTER refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A109 MUSHROOM 11am; Report refrigerated or frozen. IgE (Kukurmutta) Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A051 11am; Report PEACH (Aaru) refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A058 PINEAPPLE 11am; Report refrigerated or frozen. IgE (Ananas) Same Day Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A039 ALLERGY, PORK 11am; Report refrigerated or frozen. IgE Same Day

PAGE 17 OF 399 Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A060 11am; Report POTATO (Aloo) refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A098 RAPESEED 11am; Report refrigerated or frozen. IgE (Sarson) Same Day Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A074 ALLERGY, RYE 11am; Report refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A082 11am; Report RYEGRASS refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A037 11am; Report SESAME (Til) refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A017 11am; Report SOYBEAN refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A052 11am; Report SPINACH (Palak) refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A083 TIMOTHY 11am; Report refrigerated or frozen. IgE GRASS Same Day Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A121 ALLERGY, TUNA 11am; Report refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A042 11am; Report TURKEY refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A018 11am; Report WHEAT (Gehu) refrigerated or frozen. IgE Same Day

PAGE 18 OF 399 Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A068 11am; Report WHITE BEAN refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A033 11am; Report WILLOW refrigerated or frozen. IgE Same Day ALLERGY, CAT Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A011 EPITHELIUM & 11am; Report refrigerated or frozen. IgE DANDER Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A072 11am; Report COFFEE refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, FISH 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A031 11am; Report (Machli) refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A036 11am; Report MAIZE (Bhutta) refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A119 11am; Report MUSSEL refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A021 PEANUT 11am; Report refrigerated or frozen. IgE (Moongfali) Same Day Sample Daily by ALLERGY, RICE 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A029 11am; Report (Chawal) refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A043 TOMATO 11am; Report refrigerated or frozen. IgE (Tamatar) Same Day

PAGE 19 OF 399 Allergy Screen, 7 ALLERGY Sample Daily by Allergy Panels & 6 mL (4 mL min.) serum from 2 SST's. Ship Z089 COMPREHENSIV ImmunoCAP 11 am; Report 3 32 Individual refrigerated or frozen. E PROFILE days allergens

ALLERGY POST 7 Allergy Panels Sample Daily by 6 mL (4 mL min.) serum from 2 SST's. Ship Z092 SCREENING & 32 Individual ImmunoCAP 11 am; Report 3 refrigerated or frozen. PROFILE allergens days

Cat epithelium. Sample Daily by ALLERGY 6 mL (4 mL min.) serum from 2 SST's.Ship Z164 Cow, Dog & ImmunoCAP 11 am; Report 3 ANIMAL PANEL refrigerated or frozen. Horse Dander days Common Ragweed, Dermatophagoid es pteronyssinus, ALLERGY Dermatophagoid ASTHMA / es farinae, Sample Daily by RHINITIS 6 mL (4 mL min.) serum from 2 SST's.Ship Z685 Aspergillus ImmunoCAP 11 am; Report 3 SCREENING refrigerated or frozen. fumigatus, days PANEL Candida PEDIATRIC albicans, Penicillium notatum, Egg white, Milk & Cockroach

PAGE 20 OF 399 Wheat, Rice, Peanut, Soybean, Potato, ALLERGY Milk, ECZEMA Dermatophagoid Sample Daily by 6 mL (4 mL min.) serum from 2 SST's.Ship Z690 PANEL FOR es ImmunoCAP 11 am; Report 3 refrigerated or frozen. NON- pteronyssinus, days VEGETARIANS Dermatophagoid es farinae, Fish, Shrimp, Egg white & Meat

Wheat, Rice, Peanut, Lentil, Soybean, Potato, ALLERGY Milk, Sample Daily by ECZEMA 6 mL (4 mL min.) serum from 2 SST's.Ship Z689 Dermatophagoid ImmunoCAP 11 am; Report 3 PANEL FOR refrigerated or frozen. es pteronyssinus days VEGETARIANS & Dermatophagoid es farinae

Egg white, Milk, Sample Daily by ALLERGY FOOD 6 mL (4 mL min.) serum from 2 SST's.Ship Z691 Wheat, Soybean, ImmunoCAP 11 am; Report 3 PANEL2 refrigerated or frozen. Fish & Peanut days Orange, Apple, Banana, Peach, ALLERGY FRUIT Mango, Sample Daily by 6 mL (4 mL min.) serum from 2 SST's.Ship Z524 Strawberry, ImmunoCAP 11 am; Report 3 COMPREHENSIV refrigerated or frozen Pear, Lemon, days E PANEL Pineapple & Coconut

PAGE 21 OF 399 Strawberry, Sample Daily by ALLERGY, FRUIT 6 mL (4 mL min.) serum from 2 SST's.Ship Z693 Pear, Lemon & ImmunoCAP 11 am; Report 3 PANEL 2 refrigerated or frozen. Pineapple days Bermuda grass, Rye grass, Sample Daily by ALLERGY Timothy grass, 6 mL (4 mL min.) serum from 2 SST's.Ship Z525 ImmunoCAP 11 am; Report 3 GRASS PANEL Meadow grass, refrigerated or frozen. days Johnson grass & Bahia grass

Penicillium notatum, Cladosporium herbarum, Sample Daily by ALLERGY 6 mL (4 mL min.) serum from 2 SST's.Ship Z160 Aspergillus ImmunoCAP 11 am; Report 3 MOLD PANEL refrigerated or frozen. fumigatus, days Candida albicans & Alternaria tenuis

Pecan, Cashew Sample Daily by ALLERGY NUT nut, Pistachio, 6 mL (4 mL min.) serum from 2 SST's.Ship Z523 ImmunoCAP 11 am; Report 3 PANEL Walnut, Almond refrigerated or frozen. days & Peanut Sample Daily by ALLERGY Lentils, White 6 mL (4 mL min.) serum from 2 SST's.Ship Z522 ImmunoCAP 11 am; Report 3 PULSES PANEL bean & Chick pea refrigerated or frozen. days ALLERGY Sample Daily by 3 mL (1 mL min.) serum from 1 SST.Ship refrigerated A112 PHADIATOP, ImmunoCAP 11am; Report or frozen. PEDIATRIC Same Day Sample Daily by ALLERGY Total IgE and 3 mL (1 mL min.) serum from 1 SST.Ship refrigerated A001 ImmunoCAP 11 am; Report SCREEN Phadia Top or frozen. Same Day

PAGE 22 OF 399 Egg white, Cow's milk, Fish, Shimp, Wheat, Rice, Peanut, ALLERGY Lentil, Soybean, Sample Daily by ECZEMA Chick pea, 6 mL (4 mL min.) serum from 2 SST's.Ship Z527 ImmunoCAP 11 am; Report 3 COMPREHENSIV Lemon, Banana, refrigerated or frozen. days E PANEL Mites, Dog dander, Cow dander, Horse dander & Cat epithelium

Egg white, Milk, Wheat, Soybean, Sample Daily by ALLERGY FOOD 6 mL (4 mL min.) serum from 2 SST's.Ship Z091 Fish , Chicken, ImmunoCAP 11 am; Report 3 PANEL 1 refrigerated or frozen. Rice, Gluten, days Shrimp & Peanut ALLERGY NON- Pork, Beef, Egg, Sample Daily by 6 mL (4 mL min.) serum from 2 SST's.Ship Z520 VEGETARIAN Turkey & ImmunoCAP 11 am; Report 3 refrigerated or frozen. PANEL 1 Chicken days ALLERGY Tomato, Yeast, Sample Daily by 6 mL (4 mL min.) serum from 2 SST's.Ship Z686 VEGETABLE Garlic, Onion & ImmunoCAP 11 am; Report 3 refrigerated or frozen. PANEL 1 Celery days ALLERGY Carrot, Spinach, Sample Daily by 6 mL (4 mL min.) serum from 2 SST's.Ship Z688 VEGETABLE Potato & ImmunoCAP 11 am; Report 3 refrigerated or frozen. PANEL 3 Cucumber days

PAGE 23 OF 399 Elm, Bermuda grass, Rye grass, Timothy grass, Johnson grass, Meadow grass, Bahia grass, Alder, Mugwort, Common ALLERGY pigweed, Gluten, WHEEZE / Sample Daily by Pea, Casein, 6 mL (4 mL min.) serum from 2 SST's.Ship Z529 RHINITIS ImmunoCAP 11 am; Report 3 Housedust, refrigerated or frozen. COMPREHENSIV days Cocoa, Coffee, E PANEL ADULT Cheddar, Mesquite, Ragweed, Goosefoot, Cat epithelium, Dog dander, Cockroach, AlterneriaÇ

PAGE 24 OF 399 Elm, Lemon, Egg white, Milk, Rice, Lentil, Pea, White bean, Mesquite, Ragweed, Goosefoot, Cat ALLERGY epithelium, Dog WHEEZE / dander, Mites, Sample Daily by RHINITIS Cock roach, 6 mL (4 mL min.) serum from 2 SST's.Ship Z528 ImmunoCAP 11 am; Report 3 COMPREHENSIV Alterneria refrigerated or frozen. days E PANEL tenuis, PEDIATRIC Aspergillus fumigatus, Candida albicans, Penicillium notatum & Cladosporium herbarum

Oat, Maize, Sesame, Sample Daily by ALLERGY 6 mL (4 mL min.) serum from 2 SST's.Ship Z521 Buckwheat, Rye, ImmunoCAP 11 am; Report 3 CEREAL PANEL refrigerated or frozen. Wheat, Rice & days Carob ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A024 ACACIA 11 am; Report refrigerated or frozen. IgE (Babool) Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A075 ALMOND 11 am; Report refrigerated or frozen. IgE (Badam) Same Day

PAGE 25 OF 399 Sample Daily by ALLERGY, ANY 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific Z545 11am; Report 10 ALLERGENS refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, ANY 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific Z544 11am; Report 5 ALLERGENS refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A086 11am; Report BAHIA GRASS refrigerated or frozen. IgE Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A092 BRINJAL 11am; Report refrigerated or frozen. IgE (Baingan) Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A053 CABBAGE 11am; Report refrigerated or frozen. IgE (Bandgobi) Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A004 CANDIDA 11am; Report refrigerated or frozen. IgE ALBICANS Same Day ALLERGY Sample Daily by 3 mL (1 mL min.) serum from 1 SST.Ship refrigerated A111 PHADIATOP, ImmunoCAP 11 am; Report or frozen. ADULT Same Day Sample Daily by ALLERGY FRUIT Orange, Apple, 6 mL (4 mL min.) serum from 2 SST's.Ship Z692 ImmunoCAP 11 am; Report 3 PANEL 1 Banana & Peach refrigerated or frozen. days ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A028 ALTERNARIA 11 am; Report refrigerated or frozen. IgE TENUIS Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A064 PISTACHIO 11am; Report refrigerated or frozen. IgE (Pista) Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A120 11am; Report SALMON refrigerated or frozen. IgE Same Day

PAGE 26 OF 399 ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A020 SHRIMP (Prawn 11am; Report refrigerated or frozen. IgE / Jhinga) Same Day ALLERGY, Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A065 WALNUT 11am; Report refrigerated or frozen. IgE (Akhrot) Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A026 11am; Report WHITE PINE refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A087 11am; Report RAGWEED refrigerated or frozen. IgE Same Day Sample Daily by ALLERGY, 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A055 11am; Report STRAWBERRY refrigerated or frozen. IgE Same Day Sample Daily by 3 mL (2 ml min.) serum from 1 SST. Ship ImmunoCAP Specific A044 ALLERGY, YEAST 11am; Report refrigerated or frozen. IgE Same Day ALPHA-1- 2 mL (0.5 mL min.) serum from 1 Red Top (No Sample by ANTITRYPSIN Additive) tube. Separate serum from cells B096 Nephelometry Mon/Thu 4 pm; QUANTITATION; immediately. Ship refrigerated or frozen. Overnight Report Same day AAT fasting is preferred. ALPHA-2- 2 mL (0.5 mL min.) serum from 1 SST. Avoid Sample by Tue / B194 MACROGLOBUL hemolysis. Overnight fasting is preferred. Ship Nephelometry Fri 4 pm; IN refrigerated or frozen. ReportSame Day Sample by 7th of ALPHA 2 mL (1 mL min.) serum from 1 Red Top (No the month; RW009 SUBUNIT Additive) tube. Ship refrigerated or frozen. Reportafter 2–3 weeks

PAGE 27 OF 399 ALPHA Sample by Tue 11 THALASSEMIA 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS103 PCR am ; Report 9 MUTATION (EDTA) tube. Ship refrigerated. DO NOT FREEZE. days DETECTION ALPHA 4 mL (2 mL min.) Whole Blood in 1 Lavender top THALASSEMIA Sample by Thu (EDTA) tube each from both the parents AND 10 mL OS104 MUTATION PCR 11 am; Report 9 Amniotic fluid / CVS. Ship refrigerated. DO NOT DETECTION, days FREEZE. PRENATAL Sample by 7th of 2 mL (0.5 mL min.) serum from 1 Red Top (No ALPHA-1-ACID Fixed Rate Time the month; RW006 Additive) tube. Overnight fasting is preferred. Ship GLYCOPROTEIN Nephelometry Reportafter 2–3 refrigerated or frozen. weeks ALPHA-1- Sample by 7th of ANTITRYPSIN 2 mL (0.5 mL min.) serum from 1 Red Top (No the month; RW007 Isoelectrofocusing (AAT) Additive) tube. Ship refrigerated or frozen. Report after2–3 PHENOTYPE weeks 10 mL (5 mL min) dialysis fluid in metal free container available from LPL. Collect specimen directly into the container. Ship refrigerated or Use only specified Sample Daily by 6 ALUMINIUM, C001 frozen. Use powderless gloves during specimen ICPMS containers available pm; ReportNext DIALYSIS FLUID collection. Patients should refrain from taking from LPL. day antacids and aluminium containing medications prior to specimen collection.

PAGE 28 OF 399 Use only specified containers available 3 mL (1 mL min.) plasma from 1 metal free Royal from LPL. If Blue Top (K2EDTA) tube available from LPL. Ship gadolinium or iodine Sample Daily by 6 ALUMINIUM, refrigerated or frozen. Use powderless gloves containing contrast C002 ICPMS pm; Report Next PLASMA during specimen collection. Patients should refrain media has been day from taking antacids and aluminium containing administered, the medications prior to specimen collection. specimen should not be collected for 96 hours.

Use only specified containers available 3 mL (1.0 mL min.) serum from 1 metal free White from LPL. If Top ( Z-No Additive) tube available from LPL. Ship gadolinium or iodine Sample Daily by 6 ALUMINIUM, refrigerated or frozen. Use powderless gloves containing contrast C003 ICPMS pm; ReportNext SERUM during specimen collection. Patients shoursld media has been day refrain from taking antacids and aluminium administered, the containing medications prior to specimen collection. specimen should not be collected for 96 hours.

*Estradiol *FSH 3 mL (1 mL min.) serum from 1 SST. Collect AMENORRHOEA *LH *Prolactin specimen 4 hrs after the patient has awakened. Chemiluminescent For male patients Z094 Daily PROFILE *Progesterone* Overnight fasting is preferred. Ship refrigerated or Immunoassay use test code Z099. TSH frozen. 3 mL (1 mL min.) serum from 1 SST. Collect AMENORRHOEA *FSH *LH specimen 4 hrs after the patient has awakened. Chemiluminescent Z479 / ANDROLOGY Daily *Prolactin Overnight fasting is preferred. Ship refrigerated or Immunoassay SCREEN frozen.

PAGE 29 OF 399 Sample by 7th of 2 mL (1 mL min.) serum from 1 Red Top (No the month; RW270 AMIKACIN Additive) tube. Do not use SST gel barrier tubes. Immunoassay Reportafter 2–3 Ship refrigerated or frozen. weeks AMINO ACIDS 2 mL (1 mL min.) plasma from 1 Green Top (Sodium QUANTITATIVE, Sample by Mon / Heparin) tube. Transfer plasma into a sterile screw G175 PLASMA, 45 LC-MS/MS Wed 5 pm; capped vial. Ship refrigerated or frozen. Clinical AMINO ACIDS / Report 3days details and drug history must accompany sample. FULL PANEL 10 mL (5 mL min.) aliquot of 24 hour urine Also see LEAD; 5- collected in a dark coloured container / wrapped PORPHYRINS, AMINOLEVULINI Sample by Tue / completely with black paper or aluminium foil. Ship Column TOTAL; U099 C ACID (5 -ALA), Fri 9 am; Report refrigerated or frozen. Record 24 hour volume on Chromatography PORPHOBILINOGEN; QUANTITATIVE, Nextday test request form and urine container. Uncovered LEAD POISONING URINE vials exposed to light are unacceptable. PANEL

1 mL (0.5 mL min.) CSF in a sterile screw capped vial AMINO ACID, AND 2 mL (1 mL min.) plasma from 1 Green Top NON-KETOTIC (Sodium Heparin) tube. Transfer plasma into a Sample by Mon / HYPERGLYCINE G178 sterile screw capped vial. Ship refrigerated or LC-MS/MS Wed 5 MIA PANEL frozen. Clinical details and drug history must pm;Report 3 days QUANTITATIVE, accompany sample. CSF & Plasma specimens should CSF & PLASMA be drawn at the same time. AMINO ACID QUANTITATIVE, 1 mL (0.5 mL min.) CSF. Ship refrigerated or frozen. Sample by Mon / G176 CSF, 45 AMINO Clinical details and drug history must accompany LC-MS/MS Wed 5 pm; ACIDS / FULL sample. Report 3 days PANEL

PAGE 30 OF 399 AMINO ACIDS, 2 mL (1 mL min.) plasma from 1 Green Top (Sodium MAPLE SYRUP Sample by Mon / Heparin) tube. Transfer plasma into a sterile screw G179 URINE DISEASE LC-MS/MS Wed 5 pm; capped vial. Ship refrigerated or frozen. Clinical (MSUD) PANEL, Report 3days details and drug history must accompany sample. PLASMA

AMINO ACIDS, 1 mL (0.5 mL min.) CSF. Ship refrigerated or frozen. Thin Layer Sample by Mon / G106 QUALITATIVE, Clinical details and drug history must accompany Chromatography, Wed / Fri 4 pm; CSF sample. One dimensional Report 2 days

AMINO ACIDS, 5 mL (2 ml min.) aliquot of first morning urine in a QUALITATIVE, Thin Layer Sample by Mon / sterile screw capped container. No preservative G107 TWO Chromatography, Wed / Fri 4 pm; required. Ship refrigerated or frozen. Clinical details DIMENSIONAL, Two dimensional Report 2 days and drug history must accompany sample. URINE

5 mL (2 ml min.) aliquot of first morning urine in a sterile screw capped container. No preservative AMINO ACIDS, required AND 2 mL (1 mL min.) plasma from 1 Thin Layer Sample by Mon / QUALITATIVE, G109 Green Top (Sodium Heparin) tube. Transfer plasma Chromatography, Wed / Fri 4 URINE & into a sterile screw capped vial. Ship refrigerated or One dimensional pm;Report 2 days PLASMA frozen. Clinical details and drug history must accompany sample.

AMINO ACIDS, 5 mL (2 ml min.) aliquot of first morning urine in a QUANTITATIVE Sample by Mon / sterile screw capped container. No preservative G174 URINE, 45 LC-MS/MS Wed 5 pm; required. Ship refrigerated or frozen. Clinical details AMINO ACIDS / Report 3days and drug history must accompany sample. FULL PANEL

PAGE 31 OF 399 Also see LEAD; 5- PORPHYRINS, AMINOLEVULINI 10 mL (5 mL min.) random urine in a dark coloured Sample by Tue / Column TOTAL; U033 C ACID (5 -ALA), container / wrapped completely with black paper or Fri 9 am; Report Chromatography PORPHOBILINOGEN; QUALITATIVE, aluminium foil. Ship refrigerated or frozen. Nextday LEAD POISONING URINE PANEL AMINO ACIDS, 5 mL (2 ml min.) aliquot of first morning urine in a QUALITATIVE, Thin Layer Sample by Mon / sterile screw capped container. No preservative G104 ONE Chromatography, Wed / Fri 4 pm; required. Ship refrigerated or frozen. Clinical details DIMENSIONAL, One dimensional Report 2 days and drug history must accompany sample. URINE

*Alanine *Arginine *Citrulline 1 drop of heel prick blood each on 3 spots of filter AMINO ACIDS *Glycine paper available from LPL OR 2 mL (1 mL min.) Sample by Mon / QUANTITATIVE, Tandem Mass G165 *Leucine*Methio whole blood in 1 Lavender Top (EDTA) tube. Clinical Wed / Fri 4 pm; BLOOD, 10 Spectrometry nine *Ornithine details and drug history must accompany sample. Report 2 days AMINO ACIDS *Phenylalanine Ship refrigerated. DO NOT FREEZE. *Tyrosine *Valine

2 mL (1 mL min.) plasma from 1 Green Top (Sodium AMINO ACIDS, Thin Layer Sample by Mon / Heparin) tube. Transfer plasma into a sterile screw G105 QUALITATIVE, Chromatography, Wed / Fri 4 pm; capped vial. Ship refrigerated or frozen. Clinical PLASMA One dimensional Report 2 days details and drug history must accompany sample.

PAGE 32 OF 399 5 mL (2 ml min.) aliquot of first morning urine in a AMINO ACIDS, sterile screw capped container. No preservative QUANTITATIVE, required. AND 2 mL (1 mL min.) plasma from 1 Sample by Mon / URINE & G177 Green Top (Sodium Heparin) tube. Transfer plasma LC-MS/MS Wed 5 pm; PLASMA , 45 into a sterile screw capped vial. Ship refrigerated or Report 3days AMINO ACIDS / frozen. Clinical details and drug history must FULL PANEL accompany sample.

3 mL (1.5 mL min.) serum from 1 Red Top (No Sample by 7th of Amitryptyline & Additive) tube. Do not use SST gel barrier tubes. the month; RW012 AMITRIPTYLINE metabolite LC-MS/MS Ship refrigerated or frozen. Recommended Reportafter 2–3 Nortriptyline specimen: >12 hours post oral dose. weeks *PML RARA t(15;17)(q22;q12 ) GENE REARRANGEMEN T *AML ETO AML t(8;21) GENE 3 mL (2 mL min.) Whole blood / Bone marrow from Sample by Mon / Z535 CATEGORIZATIO REARRANGEMEN 1 Lavender Top (EDTA) tube. Ship refrigerated. DO Real Time PCR Thu 11 am; N, PCR T *Inv 16 ( p13 NOT FREEZE. ReportWed / Sat q22) / t(16;16)(p13;q22 ) GENE REARRANGEMEN T AML ETO t(8;21) GENE 3 mL (2 mL min.) Whole blood / Bone marrow from Sample by Mon / N045 REARRANGEME 1 Lavender Top (EDTA) tube. Ship refrigerated. DO Real Time PCR Thu 11 am; NT, PCR NOT FREEZE. ReportWed / Sat QUALITATIVE

PAGE 33 OF 399 Patients to report to 3 mL (2 mL min.) whole blood from 1 Lavender Top National Reference AMMONIA, (EDTA) tube. Separate plasma IMMEDIATELY and Lab, Rohini, New B061 Spectrophotometry Daily BLOOD transfer onto ice. Ship refrigerated or frozen. Avoid Delhi for testing. hemolysis. Outstation samples are not acceptable.

AMOEBIC Sample by Wed / 2 mL (0.5 mL min.) serum from 1 SST. Ship Enzyme S014 , IgG, / Sat 9 am; refrigerated or frozen. Immunoassay SERUM Report Same day

AMPHETAMINE 10 mL (5 ml min.) aliquot of random urine. Ship Lateral Flow K013 Daily SCREEN, URINE refrigerated or frozen. Immunoassay AMYLASE 10 mL (5 mL min.) aliquot of random urine. Ship U017 (DIASTASE), Spectrophotometry Daily refrigerated. DO NOT FREEZE URINE Sample by 7th of AMYLASE 3 mL (1 mL min.) serum from 1 SST. Avoid the month; RW013 Enzymatic ISOENZYMES hemolysis. Ship refrigerated or frozen. Report after 2–3 weeks 2 ml (1 mL min.) fluid in a sterile screw capped AMYLASE, P053 container. Ship refrigerated or frozen. Specify type Spectrophotometry Daily FLUID of fluid on test request form and sample container. AMYLASE, 2 mL (0.5 mL min.) serum from 1 SST. Avoid B050 Spectrophotometry Daily SERUM hemolysis. Ship refrigerated or frozen.

PAGE 34 OF 399 *Glucose (F&PP) *Urea *Creatinine *UricAcid*Choles terol *Triglycerides *CPK *HBDH *LDH *SGOT 3 mL (2 mL min.) serum from 1 SST, AND 2 mL (1.5 *SGPT *GGTP mL min) plasma from 1 Grey Top (Sodium Fluoride) ANALYZER 26; *Bilirubin, Total Spectrophotometry, tube EACH for fasting and PP glucose. Avoid use of Z015 SMA 26 TEST *Bilirubin, Direct Indirect ISE, Daily tourniquet and fist clenching during sampling. 12 PANEL *Bilirubin, Hexokinase hours overnight fasting essential. Ship refrigerated Indirect or frozen. *Protein, Total*Albumin *A:G Ratio *Alkaline Phosphatase *Calcium *Phosphorus *Sodium *PotasÇ

PAGE 35 OF 399 *Glucose (F) *Urea *Creatinine *Uric Acid*Cholesterol *SGOT *SGPT *GGTP 3 mL (2 mL min.) serum from 1 SST AND 2 mL (1.5 ANALYZER 18; *Bilirubin,Total mL min) plasma from 1 Grey Top (Sodium Spectrophotometry, Z018 SMA 18 TEST *Protein, Total Fluoride) tube. Avoid use of tourniquet and fist Indirect ISE, Daily PANEL *Albumin *A:G clenching during sampling. 12 hours overnight Hexokinase Ratio*Alkaline fasting essential. Ship refrigerated or frozen. Phosphatase *Calcium *Phosphorus*So dium *Potassium *Chloride ANCA Includes For SERIAL (ANTINEUTROP differentiation of DILUTIONS, use test Sample Daily by HIL perinuclear (p- 2 mL (1 mL min.) serum from 1 SST. Ship S171 codeS172. Also see 11 am; Report CYTOPLASMICA ANCA) and refrigerated or frozen. Overnight fasting is preferred. Assay ANCA-MPO, Same Day NTIBODIES), cytoplasmic (c- ANCAPR3. IFA ANCA) pattern. ANCA (ANTINEUTROP HIL Sample Daily by 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence Also see ANCA-MPO, S172 CYTOPLASMICA 11 am; Report refrigerated or frozen. Overnight fasting is preferred. Assay ANCA PR3. NTIBODIES), Next day IFA IN DILUTIONS

PAGE 36 OF 399 Also see ANCA-PR3; ANCA-MPO ANTINEUTROPHIL Sample by Tue / (ANTI Corresponds 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme CYTOPLASMIC Thu / Sat 9 S048 MYELOPEROXID with p-ANCA refrigerated or frozen. Overnight fasting is preferred. Immunoassay by am;Report Same ASEANTIBODIES immunofluorescence day ), EIA . Also see ANCA- ANCA-PR3 MPO; (SERIN ANTINEUTROPHIL Sample by Tue / Corresponds 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S047 PROTEINASE CYTOPLASMIC Thu / Sat 9 am; with c-ANCA refrigerated or frozen. Overnight fasting is preferred. Immunoassay 3ANTIBODIES), ANTIBODIES by Report Same day EIA immunofluorescence . 3 mL (1.5 mL min.) serum from 1 SST for each timed Synthetic ACTH specimen. Draw fasting baseline sample; administer (Synacthen) is ANDROSTENEDI 250 ug of synthetic ACTH (Synacthen) IV, draw notsupplied by Sample by Mon / ONE Chemiluminescent Z009 additional sample at 90 min. Ship refrigerated or LPL.Refer to Y Test Thu 4 pm ; STIMULATION Immunoassay frozen. Both tubes must be clearly marked with codes :Y014 Basal ReportSame day BY ACTH time drawn. Submit both tubes with 1 test request Y015 90 form. min. Y017 *Sex Hormone Sample by Mon / ANDROGEN Binding Globulin 3 mL (2 mL min.) serum from 1 SST. Specify age and Z553 CLIA Thu 4 pm; Report INDEX *Testosterone, sex on test request form. Ship refrigerated or frozen. Same day Total

PAGE 37 OF 399 In females, preferred sampling time is 1week 3 mL (1.5 mL min.) serum from 1 SST. An early before or after the Sample by Mon / ANDROSTENEDI Chemiluminescent R060 morning specimen is preferred. Ship refrigerated or menstrual period. Thu 4 pm; ONE Immunoassay frozen. Specify age and sex on test request form. Also See ReportSame day ANDROSTENEDIONES TIMULATION BY ACTH.

In females, Sample by Mon *FSH *LH preferred sampling ANDROLOGY 3 mL (2 mL min.) serum from 1 SST. Specify age and through Sat 9 Z099 *Testosterone, CLIA, RIA time isDay 2 / Day 3 PANEL sex on test request form. Ship refrigerated or frozen. am; Report Total & Free of the menstrual Same day cycle.

*Complete Electrical 3 mL (1.5 mL min.) serum from 1 SST AND 3 mL (2 Blood Count Impedence, VCS, ANEMIA PANEL mL min.) whole blood from 1 Lavender Top (EDTA) Z288 *Serum Iron Spectrophotometry, Daily BASIC tube. Ship refrigerated. Overnight fasting is Studies*Ferritin CLIA, preferred. *CRP Immunoturbidimetry

*Complete Blood Count *Serum Iron Electrical Studies*Ferritin 3 mL (1.5 mL min.) serum from 1 SST AND 3 mL (2 Impedence, VCS, ANEMIA PANEL *Protein Total mL min.) whole blood from 1 Lavender Top (EDTA) Spectrophotometry, Sample by 9 am; Z289 1 *Albumin *A:G tube. Ship refrigerated. Overnight fasting is CLIA, Report Next day Ratio*Transferrin preferred. Immunoturbidimetry *CRP , HPLC *Hemoglobin Electrophoresis

PAGE 38 OF 399 *Complete Blood Count 3 mL (1.5 mL min.) serum from 1 SST AND 3 mL (2 Electrical *Serum Iron mL min.) whole blood from 1 Lavender Top (EDTA) Impedence, VCS, Studies*Ferritin Sample Daily by 6 ANEMIA PANEL tube AND 2 mL (1 mL min.) whole blood from 1 Spectrophotometry, Z513 *Protein Total pm; Report Next 2 metal free Royal Blue Top (K2EDTA) tube available CLIA, *Albumin *A:G day from LPL. Ship refrigerated. Overnight fasting is Immunoturbidimetry Ratio*Transferrin preferred. , AAS *CRP *Lead Blood ANEUPLOIDY 5 mL ( 3 mL min.) Amniotic fluid in a sterile screw Sample by Tue / DETECTION, capped container. Ship refrigerated. DO NOT Quantitative N053 Fri 11 am; Report AMNIOTIC FREEZE. Give clinical history on Chromosome Fluorescence PCR Fri / Tue FLUID Analysis Test request form. 3 mL (2 mL min.) whole blood from 1 Lavender Top ANEUPLOIDY Sample by Tue / (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Quantitative N054 DETECTION, Fri 11 am; Report Give clinical history on Chromosome Analysis Test Fluorescence PCR BLOOD Fri / Tue request form. ANEUPLOIDY Submit curretted tissue in normal saline. Fetus not Formalin fixed Sample by Tue / DETECTION, accepted. Ship refrigerated. DO NOT FREEZE. Give Quantitative N055 specimen not Fri 11 am; Report PRODUCTS OF clinical history on Chromosome Analysis Test Fluorescence PCR preferred Fri/ Tue CONCEPTION request form. Sample by Sat 11 ANGELMAN 4 mL (2 mL min.) Whole Blood in 1 Lavender top Methylation specific OS105 am; Report 10 SYNDROME (EDTA) tube. Ship refrigerated. DO NOT FREEZE. PCR days Sample by 7th of 2 mL (1 mL min) plasma from 1 Lavender Top See also PLASMA the month; RW015 ANGIOTENSIN II Immunoassay (EDTA) tube. Ship refrigerated or frozen. RENIN, ACTIVITY. Reportafter 2–3 weeks ANGIOTENSIN 2 mL (1 mL min.) serum from 1 SST. Ship B063 CONVERTING Spectrophotometry Daily refrigerated or frozen. Overnight fasting is preferred. ENZYME; ACE

PAGE 39 OF 399 *Complete Blood Count 3 mL (2 mL min.) whole blood in 1 Lavender Top Electrical *Blood Group + (EDTA) tube AND 2 mL (1 mL min.) plasma from 1 Impedence,VCS, ANTENATAL Rh Factor *Urine Grey Top (Sodium Fluoride) tube AND 3 mL (1.5 mL Z290 EMT, Dipstick, Daily PANEL BASIC R/E *HIV-1 & 2 min.) serum from 1 SST AND 10 mL (5 mL min.) CMIA, Flocculation, *HBsAg aliquot of random urine. Ship refrigerated. DO NOT Hexokinase *VDRL*Glucose FREEZE. F / PP / R

*Complete Blood Count *Blood Group + 6 mL (3 mL min.) whole blood in 2 Lavender Top Electrical Rh Factor *Urine (EDTA) tubes AND 2 mL (1 mL min.) plasma from 1 Impedence, VCS, ANTENATAL R/E *HIV-1 & 2 Grey Top (Sodium Fluoride) tube AND 3 mL (1.5 mL Sample by 9 am; Z546 EMT, Dipstick, PANEL 1 *HBsAg min.) serum from 1 SST AND 10 mL (5 mL min.) Report Next day CMIA, Flocculation, *VDRL*Glucose aliquot of random urine. Ship refrigerated. DO NOT Hexokinase, HPLC F / PP / R FREEZE. *Hemoglobin electrophoresis

2 mL (1 mL min.) serum from 1 SST AND 3 mL (2 mL Also see ANTI B Sample Daily by 9 H046 ANTI A TITRE min.) whole blood in 1 Lavender Top (EDTA) tube. Tube TITRE & COOMBS am; Report Next Ship refrigerated. DO NOT FREEZE TEST. day

2 mL (1 mL min.) serum from 1 SST AND 3 mL (2 mL Also see ANTI A Sample Daily by 9 H047 ANTI B TITRE min.) whole blood in 1 Lavender Top (EDTA) tube. Tube Agglutination TITRE & am; Report Next Ship refrigerated. DO NOT FREEZE COOMBSTEST. day ANTI CYCLIC Chemiluminescent CITRULLINATED 2 mL (1 mL min.) serum from 1 SST. Ship B149 Microparticle Daily PEPTIDE; ANTI refrigerated or frozen. Immunoassay CCP

PAGE 40 OF 399 ANTI ds DNA For SERIAL Sample Daily by Double 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence S096 ANTIBODY, DILUTIONS use 11 am; Report Stranded, Native refrigerated or frozen. Overnight fasting is preferred. Assay CRITHIDIA IFA testcode S119. Same Day Sample by Mon ANTI - ds DNA 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme through Sat 9 S029 ANTIBODY, EIA refrigerated or frozen. Overnight fasting is preferred. Immunoassay am;Report Same day ANTI DNase B; Sample by Mon / 2 mL (1 mL min.) serum from 1 SST.Ship refrigerated Latex Enhanced B075 ANTIDEOXYRIB Thu 4 pm; Report or frozen. Nephelometry ONUCLEASE B Same day

ANTI - ds DNA Sample Daily by ANTIBODY, 3 mL (1.5 mL min.) serum from 1 SST. Ship Immunofluorescence S119 11 am; Report CRITHIDIA IFA refrigerated or frozen. Overnight fasting is preferred. Assay Nextday IN DILUTIONS ANTI IA-2; Sample by Fri 9 INSULINOMA 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S246 am; Report Next ASSOCIATED refrigerated or frozen. Immunoassay day. ANTIGEN ANTI Sample Daily by 4 MULLERIAN 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent B171 pm; Report Same HORMONE; refrigerated or frozen. Immunoassay Day AMH ANTI NMDA RECEPTOR / 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence Sample Daily by 9 S239 ANTI- refrigerated or frozen. Overnight fasting is preferred. Assay am; Report 5 days GLUTAMATE ANTIBODY

PAGE 41 OF 399 ANTI NUCLEAR ANTIBODY / Immunofluorescence For SERIAL Sample Daily by 2 mL (1 mL min.) serum from 1 SST. Ship S044 FACTOR (ANA / Assay (Hep-2 DILUTIONS, use test 11 am; Report refrigerated or frozen. Overnight fasting is preferred. ANF), IFA (HEP- Substrate) codeS109. Same Day 2)

ANTI NUCLEAR ANTIBODY / Immunofluorescence Sample Daily by FACTOR (ANA / 2 mL (1 mL min.) serum from 1 SST. Ship S109 Assay (Hep-2 11 am; Report ANF), IFA (HEP- refrigerated or frozen. Overnight fasting is preferred. Substrate) Nextday 2) IN DILUTIONS

ANTI NUCLEAR Sample Daily by 9 ANTIBODY / 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S027 am; Report Same FACTOR refrigerated or frozen. Overnight fasting is preferred. Immunoassay day (ANA/ANF), EIA

ANTI NUCLEAR ANTIBODY / Immunofluorescence Sample Daily by FACTOR (ANA / 2 mL (1 mL min.) serum from 1 SST. Ship S235 Assay (Hep-2 11 am; Report ANF), IFA (HEP- refrigerated or frozen. Overnight fasting is preferred. Substrate) Nextday 2) REFLEX TESTING

Anti-NMO (NEUROMYELITI 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence Sample Daily by 9 S238 S OPTICA ) refrigerated or frozen. Overnight fasting is preferred. Assay am; Report 5 days ANTIBODY /AQUAPORIN 4

PAGE 42 OF 399 ANTI SOLUBLE Sample by Sat 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme B154 LIVER am; Report Same refrigerated or frozen. Immunoassay ANTIGEN; SLA day Sample by Thu 9 ANTI - ss DNA 2 mL (1 mL min.) serum from 1 SST.Ship refrigerated Enzyme S097 am; Report Same ANTIBODY or frozen. Immunoassay day ANTI SPERM Sample by Wed / 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S012 ANTIBODY, Sat 9 am; Report refrigerated or frozen. Immunoassay SERUM Same day

ANTI 2 mL (1 mL min.) serum from 1 SST. Ship Also see ANTI DNase B073 STREPTOLYSIN - Immunoturbidimetry Daily refrigerated or frozen. B O (ASO) TITRE Combined assay ANTI with Anti TPO THYROGLOBULI 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent S095 Antibody (Test Code Daily N ANTIBODY; refrigerated or frozen. Immunoassay Z201) is ANTI Tg recommended *Antithyroglobuli This is the ANTI THYROID n Antibody 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent recommended test Z201 ANTIBODIES *Antithyroid Daily refrigerated or frozen. Immunoassay forThyroid PANEL Peroxidase Antibodies. Antibody

PAGE 43 OF 399 Combined assay with Anti Tg Antibody (Test Code Z201) is ANTI THYROID recommended. Anti PEROXIDASE 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent TPO replaces the S094 Daily ANTIBODY; refrigerated or frozen. Immunoassay earlier Anti Thyroid ANTI TPO Microsomal Antibody (TMA) because of greater specificity for the thyroid.

3 mL (1.5 mL min.) Platelet Poor Plasma (PPP) from 2 Blue Top (Sodium Citrate) tubes. Prepare PPP within 1 hour of collection as follows: Centrifuge sample at 3600 rpm for 15 min & transfer supernatent to a clean plastic tube. Centrifuge this supernatant again at 3600 rpm for 15 mins & finally Sample by 7th of ANTITHROMBIN transfer the supernatant (PPP) to 1 labelled, clean Fixed Rate Time the month; RW016 III, ANTIGENIC plastic screw capped vial. FREEZE IMMEDIATELY. Nephelometry reportafter 2–3 Ship frozen. DO NOT THAW. Overnight fasting is weeks. preferred. Duly filled Coagulation Requisition Form is mandatory Patient should avoid Anabolic steroids, Gemfibrozil, Heparin therapy, Asparaginase, Estrogens, Gestodene and Oral contraceptives 3 days prior to specimen collection.

PAGE 44 OF 399 It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium Citrate) discontinues tube. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral 1 hour of collection as follows: Centrifuge sample at Anticoagulants for 7 ANTITHROMBIN 3600 rpm for 15 min & transfer supernatent to a Sample by Thu 9 Photo-optical clot days prior to H101 ACTIVITY, clean plastic tube. Centrifuge this supernatant again am; Report Next detection sampling as these FUNCTIONAL at 3600 rpm for 15 mins & finally transfer the day drugs may affect supernatant (PPP) to 1 labelled, clean plastic screw testresults. capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician.

Sample by Mon APO E 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS106 PCR Hybridization 11 am; Report GENOTYPING (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. 10 days Also see LIPID PROFILE; LIPOPROTEIN 2 mL (1 mL min.) serum from 1 SST. Minimum 12 FRACTIONATION, APOLIPOPROTEI Includes Apo B / Z212 hour fasting is mandatory . Ship refrigerated or Immunoturbidimetry ULTRACENTRIFUGATI Daily NS A1 & B Apo A1 Ratio frozen. ON; HDL CHOLESTEROL SUBCLASSES; LIPOPROTEIN (a).

PAGE 45 OF 399 ARGININE 4 mL (2 mL min.) plasma from 2 Lavender Top Patient must be Sample by 7th of VASOPRESSIN / (EDTA) tubes. Draw blood in 2 pre-chilled tubes, fasting for 6 hours Extraction, the month; RW017 ANTI DIURETIC transfer in an ice bath to refrigerated centrifuge. prior to sampling. Radioimmunoassay Report after 2–3 HORMONE Separate plasma immediately and freeze within 1 No liquids including weeks (ADH), PLASMA hour. Ship frozen. DO NOT THAW water allowed.

4 mL (3 mL min.) whole blood from 1 Lavender Top Sample by Mon 4 G149 ARGINASE Spectrophotometry (EDTA) tube. Ship refrigerated. DO NOT FREEZE. pm; Report Thu

Use only specified containers available 4 mL (2 mL min.) whole blood in 1 metal free Royal from LPL. If Blue Top (K2EDTA) tube available from LPL. Ship gadolinium or iodine Sample Daily by 6 ARSENIC, refrigerated. DO NOT FREEZE. Patient should avoid containing contrast C006 ICPMS pm; Report Next BLOOD eating sea food and herbal supplements for 3 days media has been day prior to sample collection. Use powderless gloves administered, the during specimen collection. specimen should not be collected for 96 hours.

PAGE 46 OF 399 Recommended test for Arsenic 10 mL (5 mL min.) aliquot of 24-hour urine collected estimation. Use only in an acid washed (metal free) container kit specified containers available from LPL. Mix thoroughly. Transfer 10 mL available from LPL. aliquot to vial provided in kit. Measure 24 hour total Sample Daily by 6 ARSENIC, 24- Ifgadolinium or C004 volume and record on vial and test request form. ICPMS pm; Report Next HOUR URINE iodine containing Ship refrigerated or frozen. Patient should avoid day contrast media has eating sea food and herbal supplements for 3 days been administered, prior to sample collection. Use powderless gloves the specimen should during specimen collection. not be collected for 96 hours.

Use only specified containers available 10 mL (5 mL min.) aliquot of random urine collected from LPL. If in an acid washed (metal free) container available gadolinium or iodine ARSENIC, Sample Daily by 6 from LPL. Ship refrigerated or frozen. Patient should containing contrast C005 RANDOM ICPMS pm; Report Next avoid eating sea food and herbal supplements for 3 media has been URINE day days prior to sample collection. Use powderless administered, the gloves during specimen collection. specimen should not be collected for 96 hours.

PAGE 47 OF 399 Recommended as a follow up test if Anti Nuclear Antibody/Factor is positive in Z260 *CBC *CRP *RA ARTHRITIS PANEL 1. Factor*Anti Also see Electrical Nuclear 3 mL (2 mL min.) whole blood in 1 Lavender Top RHEUMATOID tests, Impedence, VCS, Sample Daily by 9 ARTHRITIS Antibody/ Factor (EDTA) tube AND 3 mL (1.5 mL min.) serum from 1 LUPUS Z261 Spectrophotometry,I am; Report Same PANEL 2 *Uric Acid SST. Ship refrigerated. DO NOT FREEZE. Overnight ERYTHEMATOSUS mmunoturbidimetry, Day *ASO*Compleme fasting is preferred. PANEL, EIA nt C3 SJOGREN'SSYNDROM *Complement C4 E ANTIBODIES, COLLAGEN DISEASES PANEL, PHOSPHOLIPID SYNDROME PANEL and DEEP VEIN Ç

PAGE 48 OF 399 This panel is recommended as initial work up for arthritis patients. Also see RHEUMATOID tests, *CBC *CRP *RA Electrical LUPUS 3 mL (2 mL min.) whole blood in 1 Lavender Top Factor*Anti Impedence,VCS, ERYTHEMATOSUS Sample Daily by 9 ARTHRITIS (EDTA) tube AND 3 mL (1.5 mL min.) serum from 1 Z260 Nuclear Spectrophotometry,I PANEL, SJOGREN'S am; ReportSame PANEL 1 SST. Ship refrigerated. DO NOT FREEZE. Overnight Antibody/ Factor mmunoturbidimetry, SYNDROME day fasting is preferred. *ASO *Uric Acid EIA ANTIBODIES, COLLAGEN DISEASES PANEL, PHOSPHOLIPID SYNDROME PANEL and DEEP VEIN THROMBOSIS PANEL.

3 mL (2 mL min.) whole blood in 1 Lavender Top Electrical *CBC *CRP *RA ARTHRITIS (EDTA) tube AND 3 mL (1.5 mL min.) serum from 1 Impedence, VCS, Z316 Factor *ASO Daily PANEL BASIC SST. Ship refrigerated. DO NOT FREEZE. Overnight Spectrophotometry,I *Uric Acid fasting is preferred. mmunoturbidimetry

ASCA (ANTI SACCHAROMYC Sample by Thu 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z675 ES CEREVISIAE am; Report Same refrigerated or frozen. Immunoassay ANTIBODY), IgA day & IgG PANEL

PAGE 49 OF 399 ASCA (ANTI Sample by Thu 9 SACCHAROMYC 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S219 am; Report Same ES CEREVISIAE refrigerated or frozen. Immunoassay day ANTIBODY), IgA

ASCA (ANTI Sample by Thu 9 SACCHAROMYC 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S218 am; Report Same ES CEREVISIAE refrigerated or frozen. Immunoassay day ANTIBODY), IgG

ASPERGILLUS Also see ALLERGY, Sample by Wed 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S102 ANTIBODY, INDIVIDUAL,ASPERGI am; Report Same refrigerated or frozen. Immunoassay IgG, SERUM LLUS. day ASPERGILLUS Also see ALLERGY, Sample by Wed 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S155 ANTIBODY, INDIVIDUAL,ASPERGI am; Report Same refrigerated or frozen. Immunoassay IgM, SERUM LLUS. day ASPERGILLUS Also see ALLERGY, Sample by Wed 9 ANTIBODIES 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z215 INDIVIDUAL,ASPERGI am; Report Same PANEL, IgG & refrigerated or frozen. Immunoassay LLUS. day IgM, SERUM Immunoassay, Sample by 7th of AUTOIMMUNE 3 mL(1.5 mL min.) serum from 1 Red Top (No Immunofluorescence the month; RW259 CEREBELLAR Additive) Tube.Do not use SST gel barrier tubes. Assay (IFA), Reportafter 2–3 ATAXIA PANEL Ship refrigerated or frozen. Immunoturbidimetry weeks , Radiobinding Assay AUTOIMMUNE *ANA *AMA Sample Daily by 3 mL (2 mL min.) serum from 1 SST. Ship Immunofluorescence Z619 HEPATITIS *ASMA *LKM 11 am; Report refrigerated or frozen. Assay PANEL Antibody Same Day

PAGE 50 OF 399 Individuals infected Sample by 7th of BABESIA 2 mL (0.5 mL min.) serum from 1 Red Top (No Immunofluorescence with malaria the month; RW019 ANTIBODIES, Additive) tube. Do not use SST gel barrier tubes. Assay mayfalsely test Reportafter 2–3 IgG & IgM Ship refrigerated or frozen. positive weeks BACTERIAL *H.influenzae 4 mL (2 mL min.) Whole Blood in 1 Lavender top Sample by Wed / MENINGITIS *N.meningitidis OS107 (EDTA) tube OR 1 mL CSF OR Throat swab. Ship PCR Sat 11 am; CONFIRMATOR * refrigerated. DO NOT FREEZE. Report Sat / Wed Y PANEL S.pneumoniae

*Mycoplasma BACTERIAL Submit Throat Swab / Nasopharyngeal swab / pneumoniae *S. Sample Daily ; OS108 PNEUMONIA Respiraory secretions / BAL fluid in a sterile screw PCR pneumoniae * Report 7 days PANEL , PCR capped container Ship refrigerated. DO NOT FREEZE. Chlymydia genus

*Group B Streptococcus *H.influenzae Type B 2 mL (1 mL min.) serum from 1 SST OR 2 mL (1 mL BACTERIAL *Streptococcus min.) Cerebrospinal fluid OR 5 mL (3 mL min.) S103 MENINGITIS pneumoniae Latex Agglutination Daily random urine in a sterile screw capped container. SCREEN *Neisseria Ship refrigerated or frozen. meningitidis Group A, C, Y, W135 & B *E.coli K1 Amobarbital, BARBITURATE Butabarbital, SCREEN, Butalbital, 10 mL (5 mL min.) aliquot of random urine. Ship Lateral Flow K014 Daily QUALITATIVE, Pentobarbital, refrigerated or frozen. Immunoassay URINE Phenobarbital and Secobarbital

PAGE 51 OF 399 B-CELL Sample by Sat 11 4 mL (2 mL min.) Whole Blood in 1 Lavender top PCR Fragment OS109 REARRANGEME am; Report 10 (EDTA) tube. Ship refrigerated. DO NOT FREEZE. analysis NT DETECTION days Sample by Mon / 5 mL (3 mL min.) Whole blood / Bone marrow in 1 BCR-ABL PCR, Wed / Fri 9 am; N035 Lavender Top (EDTA) tube. Ship refrigerated. DO Real Time PCR QUANTITATIVE Report Tue / Thu NOT FREEZE. / Sat Sample by Mon / 5 mL (3 mL min.) Whole blood / Bone marrow in 1 BCR-ABL PCR, Wed / Fri 9 am; N037 Lavender Top (EDTA) tube. Ship refrigerated. DO Real Time PCR QUALITATIVE Report Tue / Thu NOT FREEZE. / Sat BENCE JONES PROTEIN, 15 mL (10 mL min.) Urine in a sterile screw capped U103 Chemical Daily URINE, container. Ship refrigerated. DO NOT FREEZE. QUALITATIVE Chlordiazepoxide , Clonazepam, Demoxepam, Desalkylflurazepa BENZODIAZEPIN m Diazepam, E SCREEN, Flunitrazepam, 10 mL (5 mL min.) aliquot of random urine. Ship Lateral Flow K002 Daily QUALITATIVE, Flurazepam, refrigerated or frozen. Immunoassay URINE Lorazepam, Nitrazepam, Nordiazepam, Oxazepam & Triazolam BETA 2 mL (1 mL min.) plasma from 1 Lavender Top Sample by Mon 4 R168 CROSSLAPS; (EDTA) tube. Overnight fasting is mandatory. Ship ECLIA pm; Report Same BETA CTx refrigerated or frozen. day

PAGE 52 OF 399 BETA 2 Sample by Mon / 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme B178 GLYCOPROTEIN Thu 9 am; Report refrigerated or frozen. Immunoassay 1, IgM Same day BETA 2 Sample by Mon / GLYCOPROTEIN :2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z547 Thu 9 am; Report 1 PANEL, IgG, refrigerated or frozen. Immunoassay Same day IgM, IgA BETA 2 Sample by Mon / 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme B179 GLYCOPROTEIN Thu 9 am; Report refrigerated or frozen. Immunoassay 1, IgA Same day

BETA 2 Sample by Mon / 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme B177 GLYCOPROTEIN Thu 9 am; Report refrigerated or frozen. Immunoassay 1, IgG Same day

10 mL (5 mL min.) aliquot of 24-hour urine collected 6 BETA Sample by 7th of with 10g boric acid or keep urine refrigerated if HYDROXYCORTI Includes the month; RW020 collected without preservative. Record 24-hour LC-MS/MS SOL, 24-HOUR creatinine Reportafter 2–3 volume on urine container and test request form. URINE weeks Ship refrigerated or frozen. 10 mL (5 mL min.) aliquot of 24-hour urine in a BETA - 2 - sterile screw capped container. Do not use MICROGLOBULI U051 preservatives. Ship refrigerated or frozen. Record 24 CLIA Daily N, 24 HOUR hour volume on Test Request Form and urine URINE container. BETA - 2 - 2 mL (1 mL min.) serum from 1 SST. Ship R061 MICROGLOBULI refrigerated or frozen. Avoid hemolysis. Overnight CLIA Daily N, SERUM fasting is preferred.

PAGE 53 OF 399 BETA 4 mL (2 mL min.) Whole Blood in 1 Lavender top THALASSEMIA Sample by Tue *5 common (EDTA) tube from both the Parents AND 10 mL OS111 MUTATION PCR 11am; Report 10 mutations amniotic fluid / CVS. Ship refrigerated. DO NOT DETECTION , days FREEZE. PRENATAL Sample by 7th of BETA -2- 2 mL (1 mL min.) fluid in a sterile screw capped Electrophoresis, the month; RW245 TRANSFERRIN, container. Ship refrigerated or frozen. Immunofixation Reportafter 2–3 FLUID weeks BICARBONATE, 2 mL (1 mL min ) serum from 1 SST Ship Ion Selective Also see BLOOD GAS B162 Daily SERUM refrigerated or frozen. Electrode ANALYSIS Sample by BILE ACIDS, 2 mL (1 mL min.) serum from 1 SST. Ship Enzymatic Mon/Wed/Fri 9 B197 TOTAL, SERUM refrigerated or frozen. Overnight fasting is preferred. Colorimetric am; Report same day BILIRUBIN 2 mL (1 mL min.) serum from 1 SST. Ship Z106 TOTAL, DIRECT DPD, Calculated Daily refrigerated or frozen. & INDIRECT BILIRUBIN, 2 mL ( 1 mL min.) fluid in a sterile screw capped P099 DPD, Calculated Daily FLUID container. Ship refrigerated or frozen. BILIRUBIN, 2 mL (1 mL min.) serum from 1 SST. Ship B035 DPD Daily TOTAL refrigerated or frozen. BILIRUBIN, 2 mL (1 mL min.) serum from 1 SST. Ship B036 DPD Daily DIRECT refrigerated or frozen. BIOTINIDASE 2 mL (1 mL min.) serum from 1 SST OR 2 mL (1 mL Sample Daily by 4 ACTIVITY, G129 min.) plasma from 1 Green Top (Sodium Heparin) Enzyme Assay pm; Report Same QUANTITATIVE, tube. Ship refrigerated or frozen. Day BLOOD BIOTINIDASE, Sample by Tue / 1 drop of heel prick blood each on 3 spots of filter R165 NEWBORN Fluoroimmunoassay Thu / Sat 5 pm; paper available from LPL. Ship refrigerated or frozen. SCREEN Report 2 days

PAGE 54 OF 399 10 mL (5 mL min.) aliquot of 24-hour urine collected in an acid washed (metal free) container kit Sample by 7th of available from LPL. Ship refrigerated or frozen.To Inductively-Coupled BISMUTH, 24- the month; RW021 avoid potential contamination, do not measure 24- Plasma/Mass HOUR URINE Reportafter 2–3 hour volume. Avoid mineral supplements and Spectrometry weeks bismuth preparations like Pepto-Bismal for at least 1 week prior to specimen collection.

10 mL (5 mL min.) aliquot of random urine collected in an acid washed (metal free) container available Sample by 7th of BISMUTH, Inductively-Coupled from LPL. Ship refrigerated or frozen. Avoid mineral the month; RW022 RANDOM Plasma/Mass supplements and bismuth preparations like Pepto- Reportafter 2–3 URINE Spectrometry Bismal for at least 1 week prior to specimen weeks collection.

3 mL (2 mL min.) Plasma from 1 Lavender top Sample by Mon / BK VIRUS, PCR, N069 (EDTA) tube OR 15 ml (5 ml min.) Urine in a sterile Real Time PCR Thu 11 am; QUALITATIVE screw capped container. Ship refrigerated or frozen. ReportWed / Sat

3 ml (2 ml min) plasma from 1 Lavender Top (EDTA) Sample by Mon / BK VIRUS, PCR, N096 tube OR 15 ml (5 mL min.) random urine in a sterile Real Time PCR Thu 11 am; QUANTITATIVE screw capped container Ship refrigerated or frozen. ReportWed / Sat Sample by 7th of BLASTOMYCES 2 mL (0.5 mL min.) serum from 1 SST. Ship the month; RW023 ANTIBODY refrigerated or frozen. Reportafter 2–3 weeks BLEEDING Test conducted only H015 Conducted on patient. Ivy’s method Daily TIME; BT in LPL Laboratories

PAGE 55 OF 399 2 mL arterial blood in a preheparinised (1000 IU/mL) disposable syringe and needle. Avoid air BLOOD GAS ISE, Amperometry, bubbles. Mix gently 10 times. Seal needle with P001 ANALYSIS, Reflectance Daily rubber stopper. Sample to be drawn by referring ARTERIAL photometry doctor. Ship refrigerated. DO NOT FREEZE. Outstation samples not accepted. 2 mL venous blood in a pre-heparinised (1000 BLOOD GAS IU/mL) disposable syringe and needle. Avoid air ISE, Amperometry, P002 ANALYSIS, bubbles. Mix gently 10 times. Seal needle with Reflectance Daily VENOUS rubber stopper. Ship refrigerated. DO NOT FREEZE. photometry Outstation samples not accepted.

BLOOD PICTURE; 2 Peripheral blood smears. Give brief clinical H029 PERIPHERAL Microscopy Daily history. Ship in a slide mailer. BLOOD SMEAR EXAMINATION

Erythrocyte BLOOD GROUP *Forward & 4 mL (2 mL min.) whole blood in 1 Lavender Top Magnetized H206 ABO & Rh Daily Reverse grouping (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Technology / Tube FACTOR Agglutination BNP; B - TYPE 2 mL (1 mL min.) plasma from 1 Lavender Top Chemiliminescent B147 NATRIURETIC Daily (EDTA) tube. Ship refrigerated or frozen. Immunoassay PEPTIDE

PAGE 56 OF 399 3 mL (1.5 mL min.) serum from 1 SST AND 4 mL (3 mL min.) Platelet Poor Plasma (PPP) from 3 Blue Top (Sodium Citrate) tubes. Prepare PPP within 1 *Cardiolipin hour of collection as follows: Centrifuge sample at BOH (BAD Antibodies Panel 3600 rpm for 15 min & transfer supernatent to a EIA, Sample Daily by 3 OBSTETRIC Also see DEEP VEIN Z169 *Lupus clean plastic tube. Centrifuge this supernatant again Electromechanical pm; Report Next HISTORY) THROMBOSISPANEL. Anticoagulant at 3600 rpm for 15 mins & finally transfer the Clot Detection day PANEL Panel supernatant (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form is mandatory.

2 mL (1 mL min.) Bone Marrow Aspirate in 1 *Microscopy of Lavender Top (EDTA) tube AND 4 Bone Marrow Microscopy of BONE Bone Marrow Aspirate Smears AND 4 mL (2 mL min) whole blood Special stain Leishman / Jenner- Sample Daily by 1 MARROW Aspirate & in 1 Lavender Top (EDTA) tube AND 2 peripheral includes Prussian Z476 Giemsa stained pm; Report Next EXAMINATION Peripheral Blood blood smears. Air dry & fix all smears in 100% Blue Reaction for smears, Electrical day PANEL 1 *Special Stains Methanol for 30 minutes. Ship refrigerated. DO NOT Iron. Impedence, VCS *CBC FREEZE. Give clinical history on Bone Marrow Examination Test request form

2 mL (1 mL min.) Bone Marrow Aspirate in 1 *Microscopy of Lavender Top (EDTA) tube AND 4 Bone Marrow Bone Marrow Aspirate Smears AND 4 mL (2 mL min) whole blood Microscopy of Special stains BONE Aspirate & in 1 Lavender Top (EDTA) tube AND 2 peripheral Leishman /Jenner- include Prussian Sample Daily by 6 MARROW Z477 Peripheral Blood blood smears. Air dry & fix all smears in 100% Giemsa stained Blue Reaction for pm; Report 5 EXAMINATION *Special Stains Methanol for 30 minutes AND Bone Marrow smears, Electrical Iron & Reticulin days PANEL 2 *CBC * Trephine biopsy in 10% formal saline. Ship Impedence, VCS,HPE stain. Histopathology refrigerated. DO NOT FREEZE. Give clinical history on Bone Marrow Examination Test request form

PAGE 57 OF 399 BONE *Histopathology Submit specimen in 10% Formal-saline. Ship at Sample Daily by 6 MARROW, Processing, Staining, Z499 *Reticulin stain room temperature. Give clinical history on Bone pm; Report 5 TREPHINE Microscopy *Iron stain Marrow Examination Test request form. days BIOPSY PANEL Minimum 4 smears of bone marrow aspirate AND BONE Sample Daily by 2 peripheral blood smears. Fix smears in 95% Prussian Blue H227 MARROW, 12 noon; Report ethanol for 30 min and air dry. Give clinical history Reaction IRON STAIN Next day on test request form. Ship in a slide mailer. BRAF Submit formalin fixed paraffin embedded tissue Sample by Tue 11 N113 MUTATION block. Ship at room temperature. Block should have PCR, Sequencing AM, Report Mon ANALYSIS at least 10% tumor tissue. BRCA1 & Sample by 1st / *All BRCA1 & Submit 3 mL (2 mL min.) whole blood in 1 Lavender BRCA2 Genetic counselling 16th of every BRCA2 gene top (EDTA) tube. Ship refrigerated. DO NOT FREEZE. N081 MUTATION Sequencing will be providedwith month; Report mutations Consent & Clinical Information forms available from COMPREHENSIV prior appointment. 15th / 30th of mapped LPL are mandatory. E PANEL same month *BRCA1 exon2 (187delAG) * BRCA1 & BRCA1 Submit 3 mL (2 mL min.) whole blood in 1 Lavender Genetic counselling Sample Daily by 9 BRCA2 exon2(5385insc) top (EDTA) tube. Ship refrigerated. DO NOT FREEZE. SNP Genotyping will be provided N080 am; Report 8 MUTATION * BRCA2 exon11 Consent & Clinical Information forms available from using Microarray with prior weeks SCREEN (6174delT) * 5 LPL are mandatory. appointment. Experimental markers

*ER *PR Sample Daily by 6 *Includes Tumour biopsy in 10% Formal-Saline OR Formalin pmReport 5 BREAST Use test code J113 pathologist fixed tumour paraffin block OR 5 unstained sections Immunohistochemist days: Blocks / Z240 CANCER PANEL forPhotomicrograph. review for / alcohol fixed cytology smears on Poly-L-Lysine ry Sections / 1 Also see CA 15.3. presence of coated slides. Ship at room temperature. Smears7 days: malignant cells. Tissue

PAGE 58 OF 399 *ER *PR *Cathepsin D* Sample Daily by 6 Tumour biopsy in 10% Formal-Saline OR Formalin BREAST Includes pmReport5 days: fixed tumour paraffin block OR 6 unstained sections Immunohistochemist Use test code J113 Z242 CANCER PANEL pathologist Blocks / Sections / alcohol fixed cytology smears on Poly-L-Lysine ry forPhotomicrograph. 2 review for / Smears7 days: coated slides. Ship at room temperature. presence of Tissue malignant cells.

*ER *PR *HER-2 Sample Daily by 6 * Includes Tumour biopsy in 10% Formal-Saline OR Formalin pmReport 5 BREAST Use test code J113 Pathologist fixed tumour paraffin block OR 6 unstained sections Immunohistochemist days: Blocks / Z293 CANCER PANEL forPhotomicrograph. review for / alcohol fixed cytology smears on Poly-L-Lysine ry Sections / 3 Also see CA 15.3. presence of coated slides. Ship at room temperature. Smears7 days: malignant cells. Tissue

BREAST Chemiluminescent 2 mL (1 mL min.) serum from 1 SST. Ship Z388 MONITOR *CA 15.3 *CEA MicroparticleImmun Daily refrigerated or frozen. PANEL oassay Sample Daily by 6 *ER *PR *CA Tumour biopsy in 10 % Formal-Saline OR Formalin pmReport 5 15.3 *Includes fixed tumour paraffin block OR 5 unstained sections Immunohistochemist BREAST days: Blocks / pathologist / alcohol fixed cytology smears on Poly-L-Lysine ry, Use test code J113 Z387 METASTATIC Sections / review for coated slides. Ship at room temperature AND 2 mL Chemiluminescent forPhotomicrograph. MARKER Smears7 days: presence of (1 mL min.) serum from 1 SST. Ship refrigerated or Immunoassay TissueSame Day : malignant cells. frozen. CA 15.3 Sample by Tue / BRUCELLA 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S222 Fri 9 am; ANTIBODY, IgM refrigerated or frozen. Immunoassay ReportSame day

PAGE 59 OF 399 Includes Also see FEBRILE BRUCELLA Sample Daily by 6 antibody to 2 mL (0.5 mL min.) serum from 1 SST. Ship AGGLUTININSPANEL, S003 AGGLUTINATIO Tube Agglutination pm; Report Next B.abortus and refrigerated or frozen. WEIL FELIX TEST N TEST day B.melitensis andWIDAL TEST.

BRUCELLA Sample by Tue / 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z676 ANTIBODIES, Fri 9 am; refrigerated or frozen. Immunoassay IgG & IgM ReportSame day

Sample by Tue / BRUCELLA 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S221 Fri 9 am; ANTIBODY, IgG refrigerated or frozen. Immunoassay ReportSame day

Rinse mouth with water. Prepare 4 slides of Recommended test BUCCAL SMEAR scrapings from the buccal mucosa, 2 from left and 2 is Sample Daily by 6 FOR BARR J003 from right cheek. Fix immediately in 95% ethanol Aceto-orcein stain CHROMOSOMEANAL pm; Report Next BODIES (SEX for 30 min. Air dry and ship at room temperature. YSIS, BLOOD day CHROMATIN) Provide clinical details on test request form. (KARYOTYPE) BUN; BLOOD 2 mL (0.5 mL min.) serum from 1 SST. Ship B022 UREA Urease UV Daily refrigerated or frozen. NITROGEN 3 mL (1 mL min.) serum in 1 Red Top (No additive) Sample by 7th of tube. Separate serum from cells immediately. Ship the month; RW025 BUPROPION HPLC-MS/MS frozen. DO NOT THAW. Ideal sampling time is Reportafter 2-3 trough level prior to next dose. weeks CA 27.29; Sample by 7th of Also see CA 15.3, BREAST 2 mL (0.5 mL min.) serum from 1 SST. Ship the month; RW035 Immunoassay BREAST CANCER refrigerated or frozen. Reportafter 2–3 CANCERPANELS. MARKER weeks

PAGE 60 OF 399 CA 15.3; Chemiluminescent Also see CA 27.29, BREAST 2 mL (0.5 mL min.) serum from 1 SST. Ship F010 MicroparticleImmun BREAST Daily CANCER refrigerated or frozen. Provide brief clinical history. oassay CANCERPANELS MARKER CA 27.29 & CA LPL: DailyUSA: 15.3 BREAST 2 mL (1 mL min.) serum from 1 SST. Ship Sample by 7th of Z306 CANCER CMIA, Immunoassay refrigerated or frozen. the month;report MARKERS after 2–3 weeks PANEL (CA) CYFRA 21- Also see CEA & Sample by Mon 4 1; LUNG 2 mL (0.5 mL min.) serum from 1 SST. Ship Electrochemilumines NEURONE F015 pm; Report Same CANCER refrigerated or frozen. Provide brief clinical history. cence SPECIFICENOLASE Day MARKER (NSE).

10 mL (5 mL min.) aliquot of 24-hour urine collected Use only specified in an acid-washed (metal free) container kit containers available available from LPL. Mix thoroughly. Transfer 10 mL from LPL. If aliquot to vial provided in kit. Measure 24 hour total gadolinium or iodine Sample Daily by 6 CADMIUM, 24- volume and record on vial and test request form. containing contrast C008 ICPMS pm; Report Next HOUR URINE Ship refrigerated or frozen. Patient should avoid media has been day eating seafood at least 3 days prior to specimen administered, the collection. Use powderless gloves during specimen specimen should not collection. Avoid catheterized urine specimen as be collected for 96 rubber contains trace amounts of cadmium. hours.

PAGE 61 OF 399 Use only specified 10 mL (5 mL min.) aliquot of random urine collected containers available in an acid-washed (metal free) container available from LPL. If from LPL. Ship refrigerated or frozen. Patient should gadolinium or iodine CADMIUM, Sample Daily by 6 avoid eating seafood at least 3 days prior to containing contrast C009 RANDOM ICPMS pm; Report Next specimen collection. Use powderless gloves during media has been URINE day specimen collection. Avoid catheterized urine administered, the specimen as rubber contains trace amounts of specimen should not cadmium. be collected for 96 hours.

Use only specified containers available 4 mL (2 mL min.) whole blood from 1 metal free from LPL. If Royal Blue Top (K2EDTA) tube available from LPL. gadolinium or iodine Sample Daily by 6 CADMIUM, Ship refrigerated. DO NOT FREEZE. Patient should containing contrast C007 ICPMS pm; Report Next BLOOD avoid eating seafood at least 3 days prior to media has been day specimen collection. Use powderless gloves during administered, the specimen collection. specimen should not be collected for 96 hours.

CA 72.4; Sample by Mon / GASTRIC 2 mL (0.5 mL min.) serum from 1 SST. Ship Electrochemilumines F009 Thu 4 pm; CANCER refrigerated or frozen. Provide brief clinical history. cence ReportSame day MARKER 10 mL (5 mL min.) aliquot of 24 hour urine. Collect urine with 25 mL 50% concentrated HCl to maintain CALCIUM, 24 U011 pH below 3. Record 24-hour urine volume & patient Arsenazo III Daily HOUR URINE body weight on test request form and urine vial. Ship refrigerated or frozen.

PAGE 62 OF 399 CALCIUM / CREATININE 10 mL (5 mL min.) aliquot of random urine in 2 mL U096 RATIO, of 6N HCl to maintain pH below 3. Ship refrigerated Spectrophotometry Daily RANDOM or frozen. URINE 3 mL (1 mL min.) whole blood from 1 Green Top (Sodium Heparin) tube OR 2 mL (0.5 mL min.) serum CALCIUM, Ion Selective B055 from 1 SST. Avoid use of tourniquet and fist Daily IONIZED Electrode clenching during sampling. Overnight fasting is preferred. Ship refrigerated. DO NOT FREEZE. CALCIUM, 10 mL (5 mL min.) aliquot of random urine in 2 mL U073 RANDOM of 6N HCl to maintain pH below 3. Ship refrigerated Arsenazo III Daily URINE or frozen. 2 mL (0.5 mL min.) serum from 1 SST. Avoid use of CALCIUM, tourniquet and fist clenching during sampling. B042 Arsenazo III Daily SERUM Overnight fasting is preferred. Ship refrigerated or frozen. Also see 2 mL (0.5 mL min.) serum from 1 Red Top (No CALCITONIN Sample by Mon / Chemiluminescent R062 CALCITONIN Additive) tube. Ship frozen. DO NOT THAW. STIMULATIONBY Thu 4 pm; Immunoassay Overnight fasting is preferred. CALCIUM ReportSame day PENTAGASTRIN 2 mL (0.5 mL min.) serum from 1 Red Top (No Additive) tube for each timed specimen. Ship Refer to Y frozen. DO NOT THAW. All containers must be CodesY018 CALCITONIN clearly marked with time drawn and submitted with BasalY121 1 Sample by Mon / STIMULATION 1 test request form. Draw overnight fasting baseline Chemiluminescent minY019 2 Z046 Thu 4 pm; BY CALCIUM - specimen. With patient supine, administer Calcium Immunoassay min.Y020 5 ReportSame day PENTAGASTRIN Gluconate IV 2 mg/ kg body weight over 1 min, min.Y021 10 followed by Pentagastrin IV bolus 0.5 ug/kg body min.Y022 weight over 5 sec. Draw additional specimens at 1, Customized 2, 5 and 10 min.

PAGE 63 OF 399 10 mL (5 mL min.) aliquot of 2 hour urine collection in a sterile screw capped container. Follow detailed instructions as below:1) No food or drink after 6 pm a day prior to the test.2) On the day of the test do CALCIUM not void urine after 8 am.3) At 10 am void urine and EXCRETION 2 U097 discard.4) Drink a glass of water at 10 am and 11 Arsenazo III Daily HOUR FASTING am. Drink only deionized / distilled water.5) Collect URINE urine voided at 12 noon in the container with 6N HCl as the preservative.Ship refrigerated or frozen. Record 2 hour volume on the urine vial and test request form.

2 mL (0.5 mL min.) serum from 1 SST OR 3 mL (1 mL CALCIUM, min.) whole blood from 1 Green Top (Sodium Z117 TOTAL & Heparin) tube. Avoid use of tourniquet and fist ISE, Arsenazo III Daily IONIZED clenching during sampling. Overnight fasting is preferred. Ship refrigerated. DO NOT FREEZE. CALR 3 ml ( 2 ml min.) whole blood in 1 Lavender top Sample by Mon N112 MUTATION PCR, Sequencing (EDTA) tube. Ship refrigerated. DO NOT FREEZE 11 am; Report Sat DETECTION CAMPYLOBACT Sample Daily 1 5 g fresh STOOL in a sterile screw capped container. M215 ER ANTIGEN, ICT pmReport Same Ship refrigerated only. DO NOT FREEZE. STOOL day 10 mL (5 mL min.) aliqout of random urine in a Gas CANAVAN sterile screw capped container. Do not use Chromatography / Sample Daily by 9 G172 DISEASE preservatives. Ship refrigerated or frozen. Provide MassSpectrophotom am: Report 5 days brief clinical history etry Sample by 1st / Submit formalin fixed paraffin embedded tissue 15th of every CANCER 50 Next Generation N097 block. Ship at room temperature. Block should have month; Report GENE PANEL Sequencing at least 10% tumor tissue. on 15th / 30th of every month

PAGE 64 OF 399 CANDIDA 4 mL (2 mL min.) Whole Blood in 1 Lavender top Sample by Thu ALBICANS OS114 (EDTA) tube OR 1 mL CSF/Sputum/Fluids OR Tissue PCR 11 am; Report 9 DETECTION, in normal saline. Ship refrigerated. DO NOT FREEZE. days PCR

CANNABINOIDS (Delta-9-THC 10 mL (5 mL min.) aliquot of random urine. Ship Lateral Flow K003 Metabolites) / Daily refrigerated or frozen. Immunoassay MARIJUANA, SCREEN, URINE

CA 125; Chemiluminescent Also see ROMA (Risk OVARIAN 2 mL (0.5 mL min) serum from 1 SST. Ship F007 MicroparticleImmun of Daily CANCER refrigerated or frozen. Provide brief clinical history. oassay OvarianMalignancy) MARKER CA 19.9; Chemiluminescent PANCREATIC 2 mL (0.5 mL min.) serum from 1 SST. Ship F008 MicroparticleImmun Daily CANCER refrigerated or frozen. Provide brief clinical history. oassay MARKER 2 mL (0.5 mL min.) serum from 1 Red Top (No Additive) tube. Do not use SST gel barrier tubes. CARBAMAZEPIN Chemiluminescent D051 Ship refrigerated or frozen. Recommended Daily E Immunoassay specimen: just before the next dose is due at steady state. Sample by 7th of 4 mL (2 mL min.) serum from 1 Red Top (No CARBAMAZEPIN the month; RW036 Additive) tube. Do not use SST gel barrier tubes. LC-MS/MS E, FREE Report after 2–3 Ship refrigerated or frozen. weeks CARDIAC / *CPK-MB Mass * MYOCARDIAL 2 mL (1 mL min.) serum from 1 SST.Ship Z003 Troponin I * CLIA, ECLIA Daily INFARCTION refrigerated or frozen. Troponin T PANEL

PAGE 65 OF 399 Also see Sample by Mon PHOSPHOLIPID CARDIOLIPIN 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme through Sat 9 S053 SYNDROME PROFILE ANTIBODY, IgA refrigerated or frozen. Immunoassay amReport Same & DEEP VEIN day THROMBOSIS PANEL. 2 mL (1 mL min.) plasma from 1 Lavender Top Sample Daily by 5 CARDIOMETAB G181 (EDTA) tube. Ship refrigerated or frozen. Overnight LC-MS/MS pm; Report 5 OLIC PANEL fasting is mandatory. days Sample by Mon Also see CARDIOLIPIN 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme through Sat 9 S054 PHOSPHOLIPID ANTIBODY, IgM refrigerated or frozen. Immunoassay amReport Same SYNDROMEPROFILE . day Also see PHOSPHATIDYLSERIN E ANTIBODIES CARDIOLIPIN PANEL, Sample by Mon ANTIBODIES 4 mL (3 mL min.) serum from 2 SST's. Ship Enzyme PHOSPHOLIPID through Sat 9 Z102 PANEL, IgG, IgA refrigerated or frozen. Immunoassay PANEL, DEEP VEIN amReport Same & IgM THROMBOSIS PANEL day & LUPUS ANTICOAGULANT PANEL. Also see PHOSPHOLIPID Sample by Mon CARDIOLIPIN 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme SYNDROME through Sat 9 S055 ANTIBODY, IgG refrigerated or frozen. Immunoassay PROFILE & DEEP amReport Same VEIN THROMBOSIS day PANEL. 2 mL (1 mL min.) serum from 1 SST. Separate serum CARNITINE, *Carnitine Sample Daily by 9 G170 within 1 hour of collection. Ship refrigerated or LC-MS/MS SERUM *Acylcarnitine am; Report 2 days frozen.

PAGE 66 OF 399 3 mL (2 mL min.) serum from 1 Red Top (No Additive) tube. Transfer into sterile screw capped Sample by 7th of vial. Wrap in aluminium foil to protect from light. the month; RW038 CAROTENE Ship refrigerated or frozen. Overnight fasting for 12- HPLC Also see VITAMIN A. Reportafter 2–3 14 hours is mandatory. Patient must not consume weeks alcohol or Vitamin A supplement 24 hours prior to sampling.

50 mL (10 mL min.) aliquot of 24-hour urine. Collect urine with appropriate volume of 50% HCl, to maintain pH between 1-2. Do not use concentrated HCl. Record 24 hour volume on test request form and urine container. Ship refrigerated or frozen. Patient should strictly avoid Alpha-one blockers, Aminophylline, Amphetamines, Ampicillin, Beta- Includes blockers, Ephedrine, Imipramine, Methyldopa, MAO CATECHOLAMIN High Performance Also see Sample by Wed / Epinephrine, inhibitors, Nicotine, Phenacetin, Phenothiazine, U024 ES, 24-HOUR LiquidChromatograp PHEOCHROMOCYTO Sat 9 am; Report Norepinephrine Theophylline, Vasodilators, Aspirin, PAS, Alpha-2 URINE hy MAPROFILE 2 days and Dopamine. agonists, Bromocriptine, Calcium channel blockers ( long term use), Clofibrate, Propranolol, Penicillin, Reserpine, Thyroxine, Alpha-methyldopa, Isoproterenol, Labetalol, Mandelamine, Paracetamol, Cimetidine, Metoclopramide, vigorous exercise, alcoholic beverages, tea/coffee and tobacco at least 72 hours before and during specimen collection.

PAGE 67 OF 399 50 mL (10 mL min.) aliquot of 24-hour urine. Collect urine with appropriate volume of 50% HCl to maintain pH between 1-2. Do not use concentrated HCl. Record 24 hour volume on test request form and urine container. Ship refrigerated or frozen. Patient should strictly avoid Alpha-one blockers, Aminophylline, Amphetamines, Ampicillin, Beta- blockers, Ephedrine, Imipramine, Methyldopa, MAO CATECHOLAMIN High Performance Also see Sample byWed / inhibitors, Nicotine, Phenacetin, Phenothiazine, Z118 ES & VMA, 24- LiquidChromatograp PHEOCHROMOCYTO Sat 9 am; Report Theophylline, Vasodilators, Aspirin, PAS, Alpha-2 HOUR URINE hy MAPROFILE. 2 days agonists, Bromocriptine, Calcium channel blockers (long term use), Clofibrate, Propranolol, Penicillin, Reserpine, Thyroxine, Alpha-methyldopa, Isoproterenol, Labetalol, Mandelamine, Paracetamol, Cimetidine, Metoclopramide, vigorous exercise, alcoholic beverages, tea/coffee, tobacco , chocolate, vanilla & banana at least 72 hours before and during specimen collection.

PAGE 68 OF 399 8 mL (4 mL min.) plasma from 4 special Green Top (Sodium Heparin tubes containing 5 mg Sodium metabisulphite) available from LPL on request. Adhere to REQUESTED specimen volume. Draw specimen as follows: Patient should be at rest for 30 minutes after insertion of an IV cannula. Collect specimen with patient supine. Immerse specimen immediately in ice water. Separate plasma within 10 minutes, preferably in cold centrifuge, transfer into The recommended sterile screw capped vial and FREEZE immediately. test is Includes Ship frozen. DO NOT THAW. Patient should strictly High Performance CATECHOLAMINES, CATECHOLAMIN Epinephrine, Sample by Wed 9 R097 avoid drugs like Aldomet (Alpha methyldopa), LiquidChromatograp 24-HOUR URINE. ES, PLASMA Norepinephrine am; Report Fri Labetalol, Isoproterenol, Isoetharine, Alpha-one hy Also and Dopamine. blockers, Aminophylline, Amphetamines, Ampicillin, seePHEOCHROMOCY Beta-blockers, Ephedrine, Imipramine, Nicotine, TOMA PROFILE. Phenacetin, Theophylline, Phenothiazine, Vasodilators (Minoxidil, Nitrates & Hydralazine), Aspirin, PAS, Alpha-2 agonists, Bromocriptine, Calcium channel blockers (long term use), L-dopa, Cimetidine, clofibrate, MAO inhibitors, Propranolol, Penicillin, Reserpine, Thyroxine, vigorous exercise, severe stress and cigarette smoking at least 72 hrs before and during specimen collection.

PAGE 69 OF 399 50 mL (10 mL min.) aliquot of random urine. Collect urine with appropriate volume of 50% HCl , to maintain pH between 1–2. Do not use concentrated HCl. Ship refrigerated or frozen. Patient should strictly avoid Alpha-one blockers, Aminophylline, Amphetamines, Ampicillin, Beta-blockers, Ephedrine, Imipramine, Methyldopa, MAO Includes CATECHOLAMIN inhibitors, Nicotine, Phenacetin, Phenothiazine, High Performance Also see Sample by Wed / Epinephrine, U043 ES, RANDOM Theophylline, Vasodilators, Aspirin, PAS, Alpha-2 LiquidChromatograp PHEOCHROMOCYTO Sat 9 am; Report Norepinephrine URINE agonists, Bromocriptine, Calcium channel blockers hy MAPROFILE. 2 days and Dopamine. (long term use), Clofibrate, Propranolol, Penicillin, Reserpine, Thyroxine, Alpha-methyldopa, Isoproterenol, Labetalol, Mandelamine, Paracetamol, Cimetidine, Metoclopramide, vigorous exercise, alcoholic beverages, tea/coffee and tobacco at least 72 hours before and during specimen collection.

Also see C3 & C4 2 mL (1 mL min.) serum from 1 SST. Separate serum HISTOPATHOLOGY, Z108 COMPLEMENT within 1 hour of collection. Ship refrigerated or Immunoturbidimetry Daily DIRECTIMMUNOFLU PANEL frozen. Overnight fasting is preferred. ORESCENCE TESTS.

Sample by 7th of C6 2 mL (0.5 mL min.) serum from 1 SST. Ship Radial the month; RW031 COMPLEMENT refrigerated or frozen. Immunodiffusion Reportafter 2–3 weeks Sample by 7th of C9 2 mL (0.5 mL min.) serum from 1 SST. Ship Radial the month; RW034 COMPLEMENT refrigerated or frozen. Immunodiffusion Reportafter 2–3 weeks

PAGE 70 OF 399 Also see 2 mL (0.5 mL min.) serum from 1 SST. Separate C3 HISTOPATHOLOGY, H105 serum within 1 hour of collection. Ship refrigerated Immunoturbidimetry Daily COMPLEMENT DIRECTIMMUNOFLU or frozen. Overnight fasting is preferred. ORESCENCE TESTS.

Sample by 7th of C8 2 mL (0.5 mL min.) serum from 1 SST. Ship Radial the month; RW033 COMPLEMENT refrigerated or frozen. Immunodiffusion Reportafter 2–3 weeks

Also see 2 mL (0.5 mL min.) serum from 1 SST. Separate C4 HISTOPATHOLOGY, H106 serum within 1 hour of collection. Ship refrigerated Immunoturbidimetry Daily COMPLEMENT DIRECTIMMUNOFLU or frozen. Overnight fasting is preferred. ORESCENCE TESTS.

Sample by 7th of C7 2 mL (0.5 mL min.) serum from 1 SST. Ship Radial the month; RW032 COMPLEMENT refrigerated or frozen. Immunodiffusion Reportafter 2–3 weeks Sample by 7th of 2 mL (0.5 mL min.) serum from 1 SST. Separate C2 Radial the month; RW029 serum within 1 hour of collection. Ship refrigerated COMPLEMENT Immunodiffusion Reportafter 2–3 or frozen. weeks Sample by 7th of C5 2 mL (1 mL min.) serum from 1 SST. Ship Radial the month; RW030 COMPLEMENT refrigerated or frozen. Immunodiffusion Reportafter 2–3 weeks 2 g (0.5 g min.) fresh stool in a sterile screw capped C. DIFFICILE Also see CULTURE, container. Ship refrigerated. DO NOT FREEZE. For Immunochromatogr T006 TOXIN A & B, CLOSTRIDIUMDIFFICI Daily patients using diapers, first line the diaper with aphy STOOL LE. clean plastic to prevent absorption.

PAGE 71 OF 399 CEA; CARCINO Chemiluminescent 2 mL (1 mL min.) serum from 1 SST. Ship F003 EMBRYONIC MicroparticleImmun Daily refrigerated or frozen. Give brief clinical history. ANTIGEN oassay CEBPA Sample by Wed 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS115 MUTATION PCR 11 am; Report 8 (EDTA) tube. Ship refrigerated. DO NOT FREEZE. DETECTION days Also see Sample by Tue / CENTROMERE 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S079 EXTRACTABLE Fri 9 am; Report ANTIBODY refrigerated or frozen. Overnight fasting is preferred Immunoassay NUCLEARANTIGENS. Same day

Sample Mon through Sat by 4 CERULOPLASMI 2 mL (0.5 mL min.) serum from 1 SST. Overnight pm;Report Same B087 Nephelometry Also see COPPER. N fasting is preferred. Ship refrigerated or frozen. daySample Sun by 9 am: Report Same day

*Cervical PAP Collect Endocervical smears with a brush after Smear, Genital, inserting the speculum. Dip & swirl the brush Female (Liquid Microscopy of completely into the LBC vial with liquid fixative. Mix Sample by Wed / CERVICAL based) * Human Papanicolaou Do not take smears Z770 thoroughly. Use Special Collection Kit available from Sat 11 am; SCREEN Papilloma Virus stained smears, RT- during menstruation. LPL only. Ship refrigerated. DO NOT FREEZE. Brief Report Fri/ Tue (HPV) Detection PCR clinical history in Cytology Requisition Form is & Genotyping, mandatory. PCR, Qualitative

PAGE 72 OF 399 C1 ESTERASE INHIBITOR, 2 mL (0.5 mL min.) serum from 1 Red Top (No Sample by Wed 9 PROTEIN Additive) tube. Do not use gel barrier tubes. Radial H102 am; Report next QUANTITATION; Separate serum within 1 hour of collection. Ship Immunodiffusion Mon C1 INHIBITOR, refrigerated or frozen. Overnight fasting is preferred. QUANTITATIVE

C1 ESTERASE INHIBITOR, 2 mL (0.5 mL min.) serum from 1 Red Top (No Sample by 7th of FUNCTIONAL Additive) tube. Do not use gel barrier tubes. the month; RW027 Immunoassay ACTIVITY; C1 Separate serum within 1 hour of collection and Reportafter 2–3 INACTIVATOR, freeze. Ship frozen. DO NOT THAW. weeks FUNCTIONAL 3 mL (2 mL min.) Whole Blood / Plasma in 1 CHIKUNGUNYA Lavender Top (EDTA) Tube OR 2 mL (1 mL min.) Sample Daily by N131 VIRUS, PCR Serum from 1 SST OR 2 mL (1 mL min.) CSF in a Real Time PCR 11 am; Report QUALITATIVE sterile screw capped container. Ship refrigerated. Next day DO NOT FREEZE CHIKUNGUNYA 2 mL (1 mL min.) serum from 1 SST. Ship Immunochromatogr S209 VIRUS Daily refrigerated or frozen. aphy ANTIBODY, IgM CHIMERISM Sample Daily by 3 ml (2 ml min.) whole blood in 1 Lavender top PCR, Fragment N110 SPLIT CELL 11 am; Report 7 (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Analysis ANALYSIS days Indicate date of CHIMERISM, 4 ml (2 ml min) whole blood / Bone marrow in 1 transplant on Test Sample Daily by PCR, Fragment N058 POST- Lavender Top (EDTA) tube. Ship refrigerated. DO request form. Whole 11 am; Report 7 analysis ENGRAFTMENT NOT FREEZE. blood is the days preferred sample.

PAGE 73 OF 399 Whole blood is the CHIMERISM, preferred sample. Sample Daily by 4 ml (2 ml min) whole blood in 1 Lavender Top PCR, Fragment N059 PRE- Buccal swab/Nail 11 am; Report 7 (EDTA) tube. Ship refrigerated. DO NOT FREEZE analysis ENGRAFTMENT clippings also days acceptable.

CHIMERISM, Whole blood is the 4 ml (2 ml min) whole blood in 1 Lavender Top PRE- preferred sample. Sample Daily by (EDTA) tube each for Donor and Recipient. Ship PCR, Fragment N057 ENGRAFTMENT, Buccal swab/Nail 11 am; Report 7 refrigerated. DO NOT FREEZE. Indicate Donor and analysis DONOR & clippings also days Recipient on tubes appropriately. RECIPIENT acceptable. CHLAMYDOPHIL Sample by Fri 9 A 3 mL (1.5 mL min.) serum from 1 SST.Ship Enzyme S237 am; Report Same PNEUMONIAE refrigerated or frozen. Immunoassay day IgG *C.pneumoniae IgA, IgG & IgM Sample by 7th of CHLAMYDIA / *C.psittaci 3 mL (2 mL min.) serum from 1 Red Top (No Immunoflorescence the month; RW039 CHLAMYDOPHIL IgA,IgG & IgM Additive) tube. Ship refrigerated or frozen. Assay Reportafter 2–3 A SEROLOGY *C.trachomatis weeks IgA, IgG & IgM CHLAMYDIA TRACHOMATIS 15 ml (8 mL min.) first morning urine in a sterile Sample Daily by AND NEISSERIA screw capped container OR Vaginal/Endocervical Z779 Real Time PCR 11 am; Report GONORRHOEAE /Conjuctival swab in normal saline. Ship refrigerated Next day , PCR or frozen QUALITATIVE

PAGE 74 OF 399 *Chlamydia CHLAMYDIA Sample by Fri 9 trachomatis, IgG 3 mL (1.5 mL min.) serum from 1 SST.Ship Enzyme S156 IgG ANTIBODY am; Report Same *Chlamydophila refrigerated or frozen. Immunoassay PANEL day pneumoniae, IgG

CHLAMYDIA 10 mL (5 mL min.) urine in a sterile screw capped Sample Daily by Polymerase Chain N056 TRACHOMATIS, container OR Genital / Conjunctival swabs in 11 am; Report Reaction PCR normal saline. Ship refrigerated or frozen. Next day CHLAMYDIA Sample by Fri 9 3 mL (1.5 mL min.) serum from 1 SST.Ship Enzyme S236 TRACHOMATIS am; Report Same refrigerated or frozen. Immunoassay IgG day 10 mL (5 mL min.) aliquot of 24-hour urine. CHLORIDE, 24- Refrigerate during collection. Record 24-hour Indirect Ion U044 Daily HOUR URINE volume on test request form and urine container. Selective Electrode Ship refrigerated or frozen. CHLORIDE, 10 mL (5 mL min.) aliquot of random urine. Ship Indirect Ion U015 RANDOM Daily refrigerated or frozen. Selective Electrode URINE CHLORIDE, Submit 5 mL (2 mL min.) fluid in a sterile screw Indirect Ion P088 Daily FLUID capped container. Ship refrigerated or frozen. Selective Electrode

CHLORIDE, 2 mL (1 mL min.) serum from 1 SST. Ship Indirect Ion B046 Daily SERUM refrigerated or frozen. Selective Electrode

2 mL (1 mL min.) fluid in a sterile screw capped CHOLESTEROL, P054 container. Ship refrigerated or frozen. Specify type CHO-POD Daily FLUID of fluid on test request form and sample container. CHOLESTEROL, 2 mL (0.5 mL min.) serum from 1 SST. Ship Also see LIPID B025 CHO-POD Daily TOTAL refrigerated or frozen. PROFILE TESTS. CHOLINESTERAS 2 mL (0.5 mL min.) serum from 1 SST. Ship B065 Spectrophotometry Daily E, SERUM refrigerated or frozen.

PAGE 75 OF 399 Sample by Tue / CHROMOGRANI 2 mL (0.5 mL min.) plasma from 1 Lavender Top Enzyme R098 Fri 9 am; Report N A; CGA (EDTA) tube. Ship frozen. DO NOT THAW Immunoassay Same day CHROMOSOME ANALYSIS, Submit curretted tissue in normal saline. (Fetus not PRODUCTS OF If culture is accepted). Ship immediately at 18–22°C. Specimens CONCEPTION Culture, unsuccessful, FISH Sample Daily by 6 must reach LPL, New Delhi within 24 hours. Q015 REFLEX Microscopy, will be performed pm; Report 4 Formalin fixed specimen not acceptable. Give TESTING TO Karyotype, FISH for Aneuploidy weeks clinical history on Chromosome Analysis Test FISH FOR detection. request form ANEUPLOIDY DETECTION CHROMOSOME 4 mL (2 mL min.) Whole blood in 1 Lavender Top Microarray 180K ANALYSIS FOR Sample Daily by (EDTA) tube. Ship refrigerated. DO NOT FREEZE. with focus on N091 AUTISM, 11 am; Report 25 Give clinical history on Chromosome Analysis Test Autism specific MICROARRAY days request form. genes 180K

40 mg (20 mg min.) Chorionic villus biopsy collected aseptically in 10 mL transport medium available CHROMOSOME from LPL. Sample to be collected under strict Culture, Sample Daily by 6 ANALYSIS, aseptic precautions. Ship refrigerated immediately. Q018 Microscopy, pm; Report 21 CHORIONIC DO NOT FREEZE. Specimen must reach LPL New Karyotype days VILLUS Delhi within 48 hours. Test is recommended between 10-13 weeks of gestation.Consent form for Prenatal Genetic Testing is mandatory.

PAGE 76 OF 399 4 mL (2 mL min.) Cord blood in 1 Green Top (Sodium Heparin) tube.Avoid clot formation during sampling. Ship refrigerated immediately. DO NOT CHROMOSOME Culture, Sample Daily by 9 FREEZE. Specimen must reach LPL, New Delhi within Q019 ANALYSIS, Microscopy, am; Report 10 24 hrs. Test is recommended between 18-20 weeks CORD BLOOD Karyotype days of gestation. Consent form for Prenatal Genetic Testing is mandatory. Provide gestational age of patient.

Use only specified containers available 10 mL (5 mL min.) aliquot of 24-hour urine collected from LPL. If in an acid washed (metal free) container kit gadolinium or iodine CHROMIUM, available from LPL. Mix thoroughly. Transfer 10 mL Sample Daily by 6 containing contrast C012 24-HOUR aliquot to vial provided in kit. Measure 24 hour total ICPMS pm; Report Next media has been URINE volume and record on vial and test request form. day administered, the Ship refrigerated or frozen. Use powderless gloves specimen should not during specimen collection. be collected for 96 hours.

Submit tumor tissue in a sterile screw capped container in Hank's / Ringer's solution. Ship CHROMOSOME refrigerated immediately. DO NOT FREEZE. Culture, Trim tissue free of Sample Daily by 9 Q014 ANALYSIS, Specimen must reach LPL, New Delhi within 24 hrs. Microscopy, fat, normal and am; Report 21 SOLID TUMOR Formalin fixed specimen not acceptable. Give Karyotype necrotic tissue. days clinical history on Chromosome Analysis Test request form. CHROMOSOME 4 ml (2 ml min). Whole blood in 1 Green top Culture, ANALYSIS, Sample Daily by 6 (Sodium Heparin ) tube. Ship refrigerated Microscopy, High Q020 HIGH pm; Report 21 immediately. DO NOT FREEZE. Give clinical history Resolution RESOLUTION, days on Chromosome Analysis Test Request Form. Karyotype NEONATAL

PAGE 77 OF 399 Use only specified containers available from LPL. If 10 mL (5 mL min.) aliquot of random urine collected gadolinium or iodine CHROMIUM, Sample Daily by 6 in an acid washed (metal free) container available containing contrast C013 RANDOM ICPMS pm; Report Next from LPL. Ship refrigerated or frozen. Use media has been URINE day powderless gloves during specimen collection. administered, the specimen should not be collected for 96 hours.

CHROMOFIC CHROMOSOME 4 mL (2 mL min.) whole blood in 1 lavender top Affymetrix Sample Daily by 4 SNP (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Q023 CytoScan™ 750K pm; Report 10 MICROARRAY Give clinical history on Genomic Microarray Microarray days 750K, HIGH Requisition form. RESOLUTION

CHROMOTOUC Test can also be H performed on CHROMOSOME Formalin-fixed, Submit 5 mg (2 mg min.) curretted tissue in normal Sample Daily by 4 SNP Affymetrix Optima paraffin-embedded Q025 saline. Give clinical history on Genomic Microarray pm; Report 10 MICROARRAY Suite™ Microarray tissue or Products of Requisition form. days OPTIMA, conception (POC) PRODUCTS OF specimens that fail CONCEPTION to grow in culture

PAGE 78 OF 399 Use only specified containers available from LPL. If 4 mL (2 mL min.) whole blood from 1 metal free gadolinium or iodine Sample Daily by 6 CHROMIUM, Royal Blue Top (K2EDTA) tube available from LPL. containing contrast C052 ICPMS pm; Report Next BLOOD Ship refrigerated. DO NOT FREEZE. Use powderless media has been day gloves during specimen collection. administered, the specimen should not be collected for 96 hours.

Amnotic fluid: 15 mL (10 mL min.) in a sterile screw capped container. Chorionic villus: 30 mg (20 mg CHROMOTOUC min.) biopsy collected asceptically in 10 mL H transport medium available from LPL. CHROMOSOME Sample Daily by 4 Umblical Cord blood: 4 mL (2 mL min.) cord blood in Affymetrix Optima Q024 SNP pm; Report 10 1 Lavender top (EDTA) tube. Avoid clot formation Suite™ Microarray MICROARRAY days during sampling. Ship refrigerated immediately. DO OPTIMA, NOT FREEZE. Give clinical history on Genomic PRENATAL Microarray Requisition form. Consent form for Prenatal genetic testing is mandatory.

2–3 mL (1.5 mL min.) heparinized Bone Marrow OR CHROMOSOME 3 mL (2 mL min.) whole blood in 1 Green Top Culture, Robotic ANALYSIS FOR (Sodium Heparin) tube. Ship refrigerated Preferred sample is Sample Daily by 9 Q005 Microscopy, HEMATOLOGIC immediately. DO NOT FREEZE. Specimen must reach Bone Marrow . am; Report 4 days Karyotype MALIGNANCY LPL, New Delhi within 24 hrs. Give clinical history on Chromosome Analysis Test request form.

PAGE 79 OF 399 5 mL (3 mL min.) whole blood in 2 Green Top CHROMOSOME (Sodium Heparin) tubes. Ship refrigerated Culture, Robotic Sample Daily by 9 ANALYSIS Q008 immediately. DO NOT FREEZE. Specimen must reach Microscopy, am; Report 12 (KARYOTYPE), LPL, New Delhi within 24 hrs. Give clinical history Karyotype days BLOOD on Chromosome Analysis Test request form.

CHROMOSOME ANALYSIS FOR 4 mL (2 mL min.) Whole blood in 1 Lavender Top Microarray 180K Sample Daily by UNIPATERNAL (EDTA) tube. Ship refrigerated. DO NOT FREEZE. N092 with focus to detect 11 am; Report 25 DISOMY (UPD), Give clinical history on Chromosome Analysis Test UPD days MICROARRAY request form. 180K

CHROMOSOME 4 mL (2 mL min.) Whole blood in 1 Lavender Top Sample Daily by ANALYSIS, (EDTA) tube. Ship refrigerated. DO NOT FREEZE. N074 Microarray 11 am; Report 25 MICROARRAY Give clinical history on Chromosome Analysis Test days 60K request form. 2–3 ml (1.5 mL min.) heparinized bone marrow in 1 CHROMOSOME Green Top (Sodium Heparin) tube. Ship refrigerated Culture, Sample Daily by 9 Q016 ANALYSIS, immediately. DO NOT FREEZE. Specimen must reach Microscopy, am; Report 7 days PHILADELPHIA LPL, New Delhi within 24 hrs. Give clinical history Karyotype on Chromosome Analysis Test request form.

PAGE 80 OF 399 It is recommended 20 mL (15 mL min.) Amniotic fluid in a sterile screw- to discard the initial capped container. Sample to be collected under 2 - 3 mL of aspirate, CHROMOSOME strict aseptic precautions. Ship refrigerated Culture, since this "first Sample Daily by 6 ANALYSIS, immediately. DO NOT FREEZE. Specimen must Q017 Microscopy, draw" may contain pm; Report 21 AMNIOTIC reach LPL New Delhi within 48 hours. Test is Karyotype some maternal cells days FLUID recommended between 15-20 weeks of trapped within the gestation.Consent form for Prenatal Genetic Testing needle during is mandatory. insertion.

20 mL (15 mL min.) Amniotic fluid in a sterile screw- capped container. Sample to be collected under CHROMOSOME strict aseptic precautions. Ship refrigerated INTERPHASE Sample Daily by 4 immediately. DO NOT FREEZE. Specimen must Q022 PROFILING, FISH pm ; Report 7 reach LPL New Delhi within 48 hours. Test is AMNIOTIC days recommended between 15-20 weeks of gestation. FLUID Consent form for Prenatal Genetic Testing is mandatory.

CHROMOSOME Submit 5 mg (2 mg. min) Placental tissue OR Fetal INTERPHASE tissue (Facia lata / diaphragm / tendon / skin / Sample Daily by 4 Q021 PROFILING, tissue from internal organs (if fresh) / chest wall FISH pm; Report 7 PRODUCTS OF cartilage particularly if macerated) in Normal saline days CONCEPTION / 10% Formalin.. Ship refrigerated. DO NOT FREEZE.

*CBC * C- Electrical Reactive Protein 5 mL (3 mL min.) serum from 1 SST AND 3 mL (1 mL Impedence, CHRONIC * LFT *KFT *CPK min.) whole blood from 1 Lavender Top (EDTA) tube Immunoturbidimetry Z799 FATIGUE * Glucose, AND 2 mL (1 mL min.) plasma from 1 Grey Top Daily , SYNDROME fasting * Iron (Sodium Fluoride ) tube. 12 hours overnight fasting Spectrophotometry, Studies * TSH, essential. Ship refrigerated or frozen. IFCC, Hexokinase Ultrasensitive

PAGE 81 OF 399 Sample Daily by 6 CHYLE 10 mL (5 mL min.) aliquot of random urine. Ship P072 Staining, Microscopy pm; Report Next EXAMINATION refrigerated or frozen. day Patients on CIRCULATING Adriamycin must TUMOR CELLS 15 mL (7.5 mL min.) whole blood in 2 Special Cell wait a minimum of 7 Contact National RW247 (CTC) FOR save tubes available from LPL. Ship ambient Cell search system days post Reference Lab, COLORECTAL immediately. administration NewDelhi CANCER before blood is drawn for this test.

Patients on CIRCULATING Adriamycin must TUMOR CELLS 15 mL (7.5 mL min.) whole blood in 2 Special Cell wait a minimum of 7 Contact National RW248 (CTC) FOR save tubes available from LPL. Ship ambient Cell search system days post Reference Lab, PROSTATE immediately. administration NewDelhi CANCER before blood is drawn for this test.

Patients on Adriamycin must CIRCULATING 15 mL (7.5 mL min.) whole blood in 2 Special Cell wait aminimum of 7 Contact National TUMOR CELLS RW246 save tubes available from LPL. Ship ambient Cell search system days post Reference Lab, (CTC) FOR immediately. administration NewDelhi BREAST CANCER before blood is drawn for this test. Also see KIDNEY Sample Daily by 4 CITRATE, 24- 10 mL (5 mL min.) aliquot of 24-hour urine. Ship Spectrophotometry, G147 STONEFORMATION pm; Report Same HOUR URINE frozen. DO NOT THAW. Enzymatic tests. Day C-KIT Sample by Wed MUTATION 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS118 PCR 11 am; Report 9 DETECTION , (EDTA) tube. Ship refrigerated. DO NOT FREEZE. days PCR

PAGE 82 OF 399 Includes 4 mL (2 mL min.) serum from 1 Red Top (No Sample by 7th of metabolite N- Additive) tube. Do not use SST gel barrier tubes. the month; RW044 CLOMIPRAMINE LC-MS/MS Desmethylclomip Ship refrigerated or frozen. Recommended Reportafter 2–3 ramine specimen: >12 hours post oral dose. weeks 3 mL (1.5 mL min.) serum from 1 Red Top (No Sample by 7th of Additive) tube. Do not use SST gel barrier tubes. the month; RW045 CLONAZEPAM Ship refrigerated or frozen. Recommended LC-MS/MS Report after 2–3 specimen: Before the administration of the weeks nextdose. 3 mL (1.5 mL min.) serum from 1 Red Top (No Sample by 7th of Measured as Additive) tube. Do not use SST gel barrier tubes. the month; RW046 CLORAZEPATE metabolite LC-MS/MS Ship refrigerated or frozen. Draw sample before Reportafter 2–3 Nordiazepam. next dose. weeks CLOSTRIDIUM Sample by Wed / DIFFICILE 5g (3g min.) Stool in a leak proof screw capped OS119 PCR Sat 11 pm; DETECTION, container. Ship refrigerated. DO NOT FREEZE. Report Mon / Tue PCR CLOT 5 mL whole blood in a special graduated tube H037 RETRACTION available from LPL. Insert a wooden stick Manual Daily TEST immediately and permit blood to clot. CLOTTING Test conducted only H016 Conducted on patient. Lee & White Daily TIME; CT in LPL Laboratories 2 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th of Additive) tube. Ship refrigerated or frozen. the month; RW047 CLOZAPINE LC-MS/MS Recommended specimen: 0.5 to 1 hour before next Reportafter 2–3 oral dose at steady state. weeks

PAGE 83 OF 399 BT and CT will be 3 mL (1.5 mL min.) whole blood in 1 Lavender Top performed at LPL (EDTA) tube. Ship refrigerated. DO NOT FREEZE and Laboratories. It is 3 mL Whole blood in 1 Blue Top (Sodium Citrate) recommended that tube. Mix thoroughly by inversion. Transport to Lab patient discontinues *CBC *BT *CT within 4 hrs. If this is not possible, make PPP within Electrical Heparin for 1 day *Prothrombin 1 hour of collection as follows: Centrifuge sample at Impedence, Photo- and Oral Sample by Wed / COAGULATION studies Z041 3600 rpm for 15 min & transfer supernatent to a opticalClot Anticoagulants for 7 Sat 9 am; Report PROFILE 2 *APTT*Thrombin clean plastic tube. Centrifuge this supernatant again Detection, Ivy, Lee days prior to Fri/ Tue. Time*Factor VIII at 3600 rpm for 15 mins & finally transfer the & White sampling as these Functional supernatant (PPP) to 1 labelled, clean plastic screw drugs may affect capped vial. FREEZE IMMEDIATELY. Ship frozen. DO test results. NOT THAW. Overnight fasting is preferred. Duly Discontinuation filled Coagulation Requisition Form is mandatory. should be with prior consent from tÇ

3 mL (1.5 mL min.) whole blood in 1 Lavender Top (EDTA) tube. Ship refrigerated. DO NOT FREEZE AND 3 mL Whole blood in 1 Blue Top (Sodium Citrate) *CBC *BT *CT tube. Mix thoroughly by inversion. Transport to Lab *Prothrombin within 4 hrs. If this is not possible, make PPP within Electrical studies 1 hour of collection as follows: Centrifuge sample at Impedence, Photo- BT and CT will be COAGULATION Z042 *APTT*Thrombin 3600 rpm for 15 min & transfer supernatent to a opticalClot performed at Daily PROFILE 1. TimeDoes not clean plastic tube. Centrifuge this supernatant again Detection, Ivy, Lee LPLLaboratories include Factor at 3600 rpm for 15 mins & finally transfer the & White VIII assay supernatant (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form is mandatory.

PAGE 84 OF 399 3 mL (1.5 mL min.) whole blood in 1 Lavender Top (EDTA) tube. Ship refrigerated. DO NOT FREEZE AND 3 mL Whole blood in 1 Blue Top (Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hrs. If this is not possible, make PPP within *Platelet Count 1 hour of collection as follows: entrifuge sample at Photo-optical Clot COAGULATION H243 *Prothrombin 3600 rpm for 15 min & transfer supernatent to a Detection,Electrical Daily SCREEN studies *APTT clean plastic tube. Centrifuge this supernatant again Impedence at 3600 rpm for 15 mins & finally transfer the supernatant (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form is mandatory.

Use only specified containers available 4 mL (2 mL min.) whole blood in 1 metal free Royal from LPL. If Blue Top (K2EDTA) tube available from LPL. Ship gadolinium or iodine Sample Daily by 6 refrigerated. DO NOT FREEZE. Patient should avoid containing contrast C014 COBALT, BLOOD ICPMS pm; Report Next mineral supplements, Vitamin B12 or Vitamin B media has been day Complex 3 days prior to specimen collection. Use administered, the powderless gloves during specimen collection. specimen should not be collected for 96 hours.

PAGE 85 OF 399 Use only specified containers available 10 mL (5 mL min.) aliquot of random urine collected from LPL. If in an acid washed (metal free) container available gadolinium or iodine COBALT, Sample Daily by 6 from LPL. Ship refrigerated or frozen. Patient should containing contrast C016 RANDOM ICPMS pm; Report Next avoid mineral supplements, Vitamin B12 or Vitamin media has been URINE day B Complex 3 days prior to specimen collection. Use administered, the powderless gloves during specimen collection. specimen should not be collected for 96 hours.

Use only specified 10 mL (5 mL min.) aliquot of 24-hour urine collected containers available in metal free container kit available from LPL. Mix from LPL. If thoroughly. Transfer 10 mL aliquot to vial provided gadolinium or iodine in kit. Measure 24 hour total volume and record on Sample Daily by 6 COBALT, 24- containing contrast C015 vial and test request form. Ship refrigerated or ICPMS pm; Report Next HOUR URINE media has been frozen. Patient should avoid mineral supplements, day administered, the Vitamin B12 or Vitamin B Complex 3 days prior to specimen should not specimen collection. Use powderless gloves during be collected for 96 specimen collection. hours.

Measured COCAINE & 10 mL (5 mL min.) aliquot of random urine in a primarily as Lateral Flow K011 METABOLITE sterile screw capped container. Ship refrigerated or Daily metabolite Immunoassay SCREEN, URINE frozen. Benzoylecgonine.

PAGE 86 OF 399 False-negatives may occur in patients with solitary pulmonary lesions. Patients with active Sample by 7th of Histoplasmosis may COCCIDIOIDES 3 mL (1.5 mL min.) serum from 1 SST. Ship the month; RW048 Immunodiffusion generate cross ANTIBODY refrigerated or frozen. Reportafter 2–3 reactions. Elevated weeks serum antibodies may be present in patients with Meningeal coccidiocomycosis.

*ANA *Anti-ds- COLLAGEN DNA *Sm *SS- Sample by Tue / DISEASE A/Ro*SS-B/La 4 mL (2.5 mL min.) serum from 2 SST’s. Ship Enzyme Also see ARTHRITIS Z155 Fri 9 am; Report ANTIBODIES *Scl-70 *Jo-1 refrigerated or frozen. Immunoassay PANELS. Same day PANEL *Centromere *U1RNP Also see individual Sample by 7th of 3 mL (1.5 mL min.) serum from 1 SST. Separate COMPLEMENT, Automated ComplementCompon the month; RW049 serum within 1 hour of collection and freeze TOTAL (CH50) liposome lysis assay ents by name (C1 to Reportafter 2–3 immediately Ship frozen. DO NOT THAW C9) weeks

PAGE 87 OF 399 2 mL (1 mL min.) plasma each from 2 Grey Top *SMA 26 (Sodium Fluoride) tubes for Glucose F & PP AND 3 *Hemogram mL (2 mL min.) serum from 1 SST AND 3 mL (2 mL COMPLETE Sample Daily by 9 Z048 *Lipid Profile, min.) whole blood from 1 Lavender Top (EDTA) tube See individual assays BODY PANEL am; Report 3days Extended*Urine AND 10 mL (5 mL min.) aliquot of random urine. R/E Ship refrigerated. DO NOT FREEZE. 12 hours overnight fasting is mandatory.

*Hemoglobin *PCV *TLC *RBC Count *MCV *MCH *MCHC *Platelet Count COMPLETE For Peripheral *Automated DLC 3 mL (2 mL min.) whole blood in 1 Lavender Top Electrical Z021 BLOOD COUNT; smear register code Daily *Absolute (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Impedence, VCS CBC H029 separately. Differential Counts *RDWDoes not include ESR & Peripheral Smear

Sample by Mon COMT 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS121 PCR Sequencing 11 am ; Report 8 GENOTYPING (EDTA) tube. Ship refrigerated. DO NOT FREEZE. days CONNEXIN 26 Sample by Wed 4 mL (2 mL min.)Whole Blood in 1 Lavender top OS122 MUTATION PCR 11 am; Report 9 (EDTA) tube. Ship refrigerated. DO NOT FREEZE. DETECTION days CONNEXIN 30 Sample by Wed 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS123 MUTATION PCR Sequencing 11 am; Report 9 (EDTA) tube. Ship refrigerated. DO NOT FREEZE. DETECTION days

PAGE 88 OF 399 Erythrocyte Sample Daily by 9 , 2 mL (1 mL min.) serum from 1 SST. Ship H027 Magnetised am; Report Same INDIRECT refrigerated or frozen. Technology Day Erythrocyte Sample Daily by 9 COOMBS TEST, 3 mL (2 mL min.) whole blood from 1 Lavender Top H028 Magnetised am; Report Same DIRECT (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Technology Day

Use only specified containers available from LPL. If barium 10 mL (5 mL min.) aliquot of random urine collected containing contrast COPPER, Sample Daily by 6 in an acid washed (metal free) container available media has been C018 RANDOM ICPMS pm; Report Next from LPL. Ship refrigerated or frozen. Use administered, the URINE day powderless gloves during specimen collection. specimen should not be collected for 96 hours. Also see CERULOPLASMIN.

Use only specified containers available from LPL. If gadolinium, iodine 3 mL (1 mL min.) serum from 1 metal free White or barium containing Sample Daily by 6 COPPER, Top (Z-No Additive) tube available from LPL. Ship C019 ICPMS contrast media has pm; Report Next SERUM refrigerated or frozen. Use powderless gloves been administered, day during specimen collection. the specimen should not be collected for 96 hours. Also see CERULOPLASMIN.

PAGE 89 OF 399 Use only specified containers available 10 mL (5 mL min.) aliquot of 24-hour urine collected from LPL. If barium in an acid-washed (metal free) container kit containing contrast available from LPL. Mix thoroughly. Transfer 10 mL Sample Daily by 6 COPPER, 24- media has been C017 aliquot to vial provided in kit. Measure 24 hour total ICPMS pm; Report Next HOUR URINE administered, the volume and record on vial and test request form. day specimen should not Ship refrigerated or frozen. Use powderless gloves be collected for 96 during specimen collection. hours. Also see CERULOPLASMIN.

2 mL (1 mL min.) serum from 1 Red Top (No Additive) tube. Diurnal variation present; take CORTICOSTERO sample between 8–10 a.m OR 4–6 p.m. Specify Sample Daily by 4 G199 LC-MS/MS NE time and date on specimen container and test pm; Report2 days request form. Overnight fasting is preferred. Ship refrigerated or frozen. 10 mL (5 mL min.) aliquot of 24-hour urine. Collect CORTISOL, urine with 10 g of boric acid to maintain sample Chemiluminescent U021 FREE, 24- integrity. Record 24 hour volume on test request Daily Immunoassay HOUR URINE form and urine container. Ship refrigerated or frozen. 2 mL (1 mL min.) serum from 1 SST. Take sample CORTISOL, between 11 p.m–12 midnight. Specify time and date Chemiluminescent R043 MIDNIGHT, on sample container and test request form. Ship Daily Immunoassay SERUM refrigerated or frozen. Four hours fasting is recommended.

PAGE 90 OF 399 2 mL (1 mL min.) serum from 1 SST for each timed specimen. Recommended timings: Morning: 7-9 Also see CORTISOL, CORTISOL, a.m; Evening: 3-5 p.m. Overnight fasting is preferred STIMULATION BY MORNING & Chemiluminescent Z075 for the morning specimen, four hours fasting is ACTH; CORTISOL Daily EVENING, Immunoassay recommended for the evening specimen. Specify SUPPRESSION BY SERUM time and date of collection on container and test DEXAMETHASONE request form. Ship refrigerated or frozen. Synthetic ACTH ( 2 mL (0.5 mL min.) serum from 1 SST for each timed Synacthen) is specimen . Draw fasting baseline sample; administer notsupplied by CORTISOL 250 ug of synthetic ACTH (Synacthen) IV, draw LPL.Refer to Y Test Chemiluminescent Z010 STIMULATION additional samples at 30 and 60 min. Ship codes :Y028 Daily Immunoassay BY ACTH refrigerated or frozen. All tubes must be clearly BasalY029 30 marked with time drawn. Submit all tubes with 1 min.Y030 60 test request form. min.Y031 Customized 2 mL (1 mL min.) serum from 1 SST. Diurnal Also see CORTISOL, CORTISOL, variation present; take specimen between 7-9 a.m. STIMULATIONBY SERUM Chemiluminescent R016 Specify time and date on sample container and test ACTH; CORTISOL Daily (MORNING Immunoassay request form. Ship refrigerated or frozen. Overnight SUPPRESSIONBY SAMPLE) fasting is preferred. DEXAMETHASONE. 2 mL (1 mL min.) serum from 1 Red Top (No Additive) tube. Diurnal variation present; take CORTISONE, sample between 8–10 a.m OR 4–6 p.m. Specify Sample Daily by 4 G198 LC-MS/MS SERUM time and date on specimen container and test pm; Report2 days request form. Overnight fasting is preferred. Ship refrigerated or frozen.

PAGE 91 OF 399 CORTISOL SUPPRESSION BY Administer tablet 1 mg Dexamethasone orally at 11 Patient should not Chemiluminescent Z058 DEXAMETHASO p.m. at night. Draw 2 mL (1 mL min.) serum from 1 be on Daily Immunoassay NE, SST at 8 am next morning. steroidmedication. OVERNIGHT LOW DOSE 10 mL (5 mL min.) aliquot of 24-hour urine in 10 g CORTISONE boric acid in a screw capped container OR keep Sample by 7th of (COMPOUND urine refrigerated during collection if preservative is the month; RW054 LC-MS/MS E), 24-HOUR not used. Record 24- hour volume on test request reportafter 2–3 URINE form and specimen container. Ship refrigerated or weeks. frozen. 2 mL (1 mL min.) serum from 1 Red Top (No Additive) tube. Diurnal variation present; take CORTISONE : *Cortisone sample between 8–10 a.m OR 4–6 p.m. Specify Sample Daily by 4 G200 CORTISOL LC-MS/MS *Cortisol *Ratio time and date on specimen container and test pm; Report2 days RATIO request form. Overnight fasting is preferred. Ship refrigerated or frozen. Sample by 7th of CORTISOL 2 mL (1 mL min.) serum from 1 Red Top (No LC-MS/MS, the month; RW053 FREE, SERUM Additive) tube. Ship refrigerated or frozen. Equilibrium Dialysis Report after 2–3 weeks 5ml (2ml min) saliva in a sterile screw capped container.Collect specimen before brushing teeth. The ideal sampling Sample by Mon 9 CORTISOL, Avoid stress, exercise, eating & drinking 30 minutes time is late at night R175 ECLIA am; Report Same SALIVA prior to sample collection. Rinse mouth thoroughly between 11pm to day before collecting sample. Ship referigerated or 12 midnight. frozen.

PAGE 92 OF 399 CORTISOL Day 1: 2 mL (1 mL min.) serum from 1 SST. Draw Patient should not SUPPRESSION baseline Cortisol sample at 8 a.m.Administer tablet be on steroid BY 4 mg Dexamethasone orally at 11 p.m. at night.Day Chemiluminescent medication.Refer to Z059 DEXAMETHASO 2: Draw 2nd specimen at 8 a.m next morning.Both Daily Immunoassay Y codesY038 NE, containers must be marked with time and date BasalY039 Post OVERNIGHT drawn, and submitted with one test request form. Dexamethasone HIGH DOSE Ship refrigerated or frozen.

2 mL (1 mL min.) serum from 1 SST. Diurnal Also see CORTISOL, CORTISOL, variation present; take sample between 3-5 p.m. STIMULATIONBY SERUM Chemiluminescent R017 Specify time and date on sample container and test ACTH; CORTISOL Daily (EVENING Immunoassay request form. Ship refrigerated or frozen. Four SUPPRESSIONBY SAMPLE) hours fasting is recommended. DEXAMETHASONE Sample by Mon 9 COXSACKIE 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S110 am; Report Same ANTIBODY, IgG refrigerated or frozen. Immunoassay Day Sample by Mon 9 COXSACKIE 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S176 am; Report Same ANTIBODY, IgM refrigerated or frozen. Immunoassay Day Sample by 7th of COXSACKIE A Includes 2, 4, 7, 2 mL (1 mL min.) serum from 1 SST.Ship refrigerated Complement the month; RW056 ANTIBODY 9, 1 & 16 or frozen. Fixation Reportafter 2–3 PANEL weeks COXSACKIE Sample by Mon 9 ANTIBODIES, 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z217 am; Report Same IgG & IgM refrigerated or frozen. Immunoassay Day PANEL

PAGE 93 OF 399 Glucagon is not supplied by 2 mL (0.5 mL min.) serum from 1 SST for each timed LPL.Refer to Y specimen. Draw baseline fasting specimen. Codes:Y041 C-PEPTIDE Administer 1 mg Glucagon I.V. Draw additional Chemiluminescent Basal Y042 Z276 STIMULATION specimens at 5, 10 and 15 minutes post-Glucagon. Daily Immunoassay 5 min. BY GLUCAGON Note time drawn on each container label and test Y043 10 min. request form. 12 hours overnight fasting mandatory Y044 15 . Ship refrigerated or frozen. min. Y045 Customized Also see GLUCOSE C-PEPTIDE, 2 mL (0.5 mL min.) serum from 1 SST. Ship Chemiluminescent TOLERANCE TEST R055 FASTING, refrigerated or frozen. Overnight fasting is Daily Immunoassay WITH C-PEPTIDE SERUM mandatory. (Z037). C-PEPTIDE, Also see GLUCOSE POST 2 mL (0.5 mL min.) serum from 1 SST 2 hours after Chemiluminescent TOLERANCE TEST R157 Daily PRANDIAL, starting meal. Ship refrigerated or frozen. Immunoassay WITH C-PEPTIDE SERUM (Z037).

2 mL (1 mL min.) plasma from 1 GreyTop (Sodium Fluoride) tube AND 2 mL (0.5 mL min.) refer to Y corresponding serum from 1 SST for each timed CodesY074 specimen. Draw baseline fasting specimen. Dissolve BasalY075 0.5 C-PEPTIDE 75g Glucose in adults / 1.75 g / Kg body weight in Chemiluminescent hrY076 1.0 hrY077 RESPONSE TO children (not exceeding 75g) in 300 mL water. Z037 Immunoassay,Hexoki 1.5 hrY078 2.0 Daily GLUCOSE, 5 Administer orally over a period of 5 minutes. Draw nase hrY079 2.5 hrY080 HOURS additional specimens at 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.0 hrY081 4.0 4.0 & 5.0 hours. Note time drawn and test required hrY082 5.0 hrY083 on each container label and test request form. Customized Overnight fasting mandatory. Ship frozen. DO NOT THAW. Urine specimens NOTREQUIRED.

PAGE 94 OF 399 5 mL (1 mL min.) aliquot of 24 hour urine. Do not C-PEPTIDE, 24- use preservatives. Record 24 hr urine volume on Chemiluminescent U095 Daily HOUR URINE container and Test Request Form. Ship frozen. DO Immunoassay NOT THAW. Also see CPK 2 mL (0.5 mL min.) serum from 1 SST. Hemolysed ISOENZYME CPK; CREATINE B029 specimens are not acceptable. Ship refrigerated or IFCC ELECTROPHORESIS, Daily KINASE frozen. ALDOLASE &CARNITINE. CPK See ALDOLASE & 2 mL (1.5 mL min.) fresh serum from 1 SST. Sample Wed by 9 ISOENZYME Includes Total Agarose gel CARNITINE for E007 Hemolysed specimens are not acceptable. Ship am; Report Same ELECTROPHORE CPK. Electrophoresis Muscular refrigerated or frozen. day SIS Dystrophies. 2 mL (1 mL min.) serum from 1 SST. Hemolysed R169 CPK-MB MASS specimens are not acceptable. Ship refrigerated or ECLIA Daily frozen. 2 mL (1 mL min.) serum from 1 SST. Hemolysed Also see CPK Enzymatic B051 CPK-MB specimens are not acceptable. Ship refrigerated or ISOENZYME Daily Immunoinhibition frozen. ELECTROPHORESIS. Sample by 7th of 2 mL (0.5 mL min.) serum from 1 SST. Separate C1q Radial the month; RW028 serum within 1 hour of collection. Ship refrigerated COMPLEMENT Immunodiffusion Reportafter 2–3 or frozen. weeks C-REACTIVE 2 mL (0.5 mL min.) serum from 1 SST. Ship B074 Immunoturbidimetry Daily PROTEIN; CRP refrigerated or frozen. Overnight fasting is preferred.

C-REACTIVE 2 mL (0.5 mL min.) serum from 1 SST. Ship B134 PROTEIN, Immunoturbidimetry Daily refrigerated or frozen. CARDIO; hsCRP CREATININE, 10 mL (5 mL min.) aliquot of random urine in a Compensated Jaffe's U045 RANDOM sterile screw capped container.. Ship refrigerated or Reaction IDMS Daily URINE frozen. Traceable

PAGE 95 OF 399 10 mL (5 mL min.) aliquot of 24-hour urine AND 2 CREATININE Includes serum mL (0.5 mL min.) serum from 1 SST. Record patient Compensated Jaffe's Z107 CLEARANCE and urine height, weight and total 24-hour urinary volume on Reaction IDMS Daily TEST creatinine. test request form and urine container. Ship Traceable refrigerated or frozen. Compensated Jaffe's CREATININE, 2 mL (0.5 mL min.) serum from 1 SST. Ship B023 Reaction IDMS Daily SERUM refrigerated or frozen. Traceable Compensated Jaffe's CREATININE, 10 mL (5 mL min.) fluid in a sterile screw capped P082 Reaction IDMS Daily FLUID container. Ship refrigerated or frozen. Traceable 10 mL (5 mL min.) aliquot of 24-hour urine. Record CREATININE, Compensated Jaffe's 24-hour volume and patient weight on test request U007 24-HOUR Reaction IDMS Daily form and urine container. Ship refrigerated or URINE Traceable frozen. CRYOGLOBULIN 3 mL (2 mL min.) whole blood from 1 Lavender Top Sample Daily by 9 H115 S QUALITATIVE Precipitation method (EDTA) tube. Ship at 18-22° C. DO NOT FREEZE. am; Report 5 days TEST

3 mL (1.5 mL min.) serum. Collect blood in a Immunofixation PREWARMED Red Top (No Additive) tube using a Strictly adhere to CRYOGLOBULIN Electrophoresis PRE-WARMED syringe and needle at 37°C. Allow to Immunofixation temperature Sample Daily by 9 H117 S PANEL for classification clot and centrifuge preferably at 37°C. Separate Electrophoresis requirements for am; Report 5 days of Cryoglobulins serum and transport at room temperature. DO NOT sampling. FREEZE. CRYPTOCOCCUS 2 mL (0.5 mL min.) serum from 1 Red Top (No Sample by Wed 9 Enzyme S112 ANTIGEN, Additive) tube. Do not use SST gel barrier tubes. am; Report Same Immunoassay SERUM Ship refrigerated or frozen. day CRYPTOSPORIDI UM / Includes Conventional Sample Daily by 5 g (1 g min.) of fresh stool in special transport T011 ISOSPORAIDENT Modified Ziehl microscopic 3.30 pm; Report medium available from LPL. Ship at 18–22°C. IFICATION, Neelsen stain. examination Same Day STOOL

PAGE 96 OF 399 Sample by Wed 9 CRYPTOCOCCUS 2 mL (0.5 mL min.) Cerebrospinal fluid in a sterile Enzyme S111 am; Report Same ANTIGEN, CSF screw capped container. Ship refrigerated or frozen. Immunoassay day CRYPTOSPORIDI Sample Daily by 1 5 g fresh STOOL in a sterile screw capped container. M202 UM ANTIGEN, ICT pm;Report Same Ship refrigerated only. DO NOT FREEZE. STOOL day Sample by 7th of 2 mL (0.5 mL min.) serum from 1 Red Top (No CRYPTOCOCCUS Also see CULTURE, the month; RW057 Additive) tube. Separate serum immmediately. Ship Tube Agglutination ANTIBODY FUNGUS Reportafter 2–3 refrigerated or frozen. weeks CRYTOCOCCUS 4 mL (2 mL min.) Whole Blood in 1 Lavender top Sample Daily by NEOFORMANS OS124 (EDTA) tube OR 1 mL CSF/Sputum/Fluids OR Tissue PCR 11 am; Report DETECTION, in normal saline. Ship refrigerated. DO NOT FREEZE. 18 days PCR

Also see MULTIPLE SCLEROSIS PANELs; MYELIN- ASSOCIATED CSF PROTEIN GLYCOPROTEIN; ELECTROPHORE Sample Daily by 9 2 mL (1.5 mL min.) CSF in a sterile screw capped Agarose gel MYELIN ASSOCIATED E004 SIS / am; Report Next container. Ship refrigerated. DO NOT FREEZE. Electrophoresis GLYCOPROTEIN OLIGOCLONAL day SULFATED BANDS GLUCURONIC PARAGLOBOSIDE AUTOANTIBODY, IgM.

PAGE 97 OF 399 Also see MULTIPLE SCLEROSIS PANELs; MYELIN- CSF & SERUM ASSOCIATED PROTEIN 2 mL (1.5 mL min.) CSF in a sterile screw capped GLYCOPROTEIN; Agarose gel / Sample Daily by 9 ELECTROPHORE container AND 2 mL (1.5 mL min.) serum from 1 MYELIN ASSOCIATED E018 Capillary am; Report Next SIS / SST. Serum should be separated within 1 hour GLYCOPROTEIN Electrophoresis day OLIGOCLONAL ofcollection. Ship refrigerated. DO NOT FREEZE. SULFATED BANDS GLUCURONICPARAG LOBOSIDE AUTOANTIBODY, IgM.

Collect 8-10 mL blood aseptically in special Plus Sample Daily by Includes Rapid Automated CULTURE, Aerobic bottle available from LPL. For children less Also see CULTURE, 3.30 pmReport ANTIBIOTIC Aerobic Culture, M010 AEROBIC, than 12 yrs collect 2 mL blood in Special Paeds Plus ANAEROBIC,BLOOD, Interim: 24 & 48 SENSITIVITY if Identification and BLOOD, RAPID bottle available from LPL. Mix by gentle swirling. RAPID. hrs Final: indicated. Sensitivity Ship at room temperature. 6 days

Includes Ear swab innoculated in special Aerobic Transport Conventional CULTURE, Sample Daily by ANTIBIOTIC Medium available from LPL. Specify left or right ear Culture, Automated M015 AEROBIC, EAR 3.30 pm; Report SENSITIVITY if swab on transport medium vial and test request Identification & SWAB 3 days indicated. form. Ship refrigerated. Sensitivity

PAGE 98 OF 399 This test code includes Bone Marrow & body Collect 8-10 mL body fluids aseptically in special fluids from sterile Sample Daily by CULTURE, Includes Plus Aerobic bottle available from LPL. For children Rapid Automated sites 3.30 pmReport AEROBIC, ANTIBIOTIC less than 12 yrs OR low fluid volumes, collect 3 mL Aerobic Culture, M133 (Pleural/Pericardial/ Interim: 24 & 48 BODY FLUIDS, SENSITIVITY if (0.5 mL min.) in Special Paeds Plus bottle available Identification and Synovial/Ascitic/CSF/ hrs Final: RAPID indicated. from LPL. Mix by gentle swirling. Ship at room Sensitivity / Ocular). This test 6 days temperature. code does not include Urine / Pus specimens.

Includes Eye swab innoculated in special Aerobic Transport Conventional CULTURE, Sample Daily by ANTIBIOTIC Medium available from LPL. Specify left or right eye Culture, Automated M014 AEROBIC, EYE 3.30 pm; Report SENSITIVITY if swab on transport medium vial & test request form. Identification & SWAB 3days indicated. Ship refrigerated. Sensitivity

CULTURE, Submit catheter tip in sterile screw capped Conventional Includes AEROBIC, container / 5 mL (1 mL min.) body effusion, culture and Sample Daily by ANTIBIOTIC P058 MISCELLANEOU aspirates, etc., in sterile screw capped container OR Automated 3.30 pm; Report SENSITIVITY if S WITH GRAM swab in Aerobic Transport medium. Specify Sensitivity, Gram 3days indicated. STAIN collection site on container and test request form. stain

Includes Submit first morning mid stream urine in a special Conventional Sample Daily by CULTURE, ANTIBIOTIC urine collection vacutainer kit available from LPL as Culture, Automated M007 3.30 pm; Report URINE SENSITIVITY if per instructions enclosed OR sterile screw capped Identification & 3days indicated. container. Ship refrigerated. Sensitivity

Includes Conventional Sample Daily by CULTURE, ANTIBIOTIC Submit 2-5 mL (1 mL min.) CSF in a sterile screw Culture, Automated M145 3.30 pm; Report AEROBIC, CSF SENSITIVITY if capped container. Ship at 18–22°C. Identification & 3 days indicated. Sensitivity

PAGE 99 OF 399 Submit nasal / throat swab (mucus membrane of Does not include Sample Daily by CULTURE, pharynx / tonsils) obtainedby rubbing over affected M078 ANTIBIOTIC Conventional culture 3.30 pm; Report DIPHTHERIA / localised lesion, in Amie's transport medium SENSITIVITY. 3days available from LPL.Ship refrigerated. Submit swab from urethra, anus, eye, endocervix or Conventional Does not include Sample Daily by CULTURE, pharynx in Amie's charcoal medium available from culture and M018 ANTIBIOTIC 3.30 pm; Report GONOCOCCUS LPL, Specify collection site on transport container Automated SENSITIVITY. 3days and test request form. Ship at room temperature. identification Sample Daily by Does not Submit pus, intrauterine devices, exudate,CSF, 3.30 pm;Report CULTURE, include pleural or other fluids, tissues in an Anaerobic Anaerobic culture M072 Interim : 1 week ACTINOMYCES ANTIBIOTIC transport medium available from LPL. Ship at on specific media Final : 3 SENSITIVITY. 18–22°C. weeks Includes Nasal swab innoculated in special Aerobic Transport Conventional CULTURE, Sample Daily by ANTIBIOTIC Medium available from LPL. Specify left or right Culture, Automated M017 AEROBIC, 3.30 pm; Report SENSITIVITY if nasal swab on transport medium vial and test Identification & NASAL SWAB 3days indicated. request form. Ship refrigerated. Sensitivity

Includes Conventional Submit pus in sterile screw capped container OR Sample Daily by CULTURE, ANTIBIOTIC Culture, Automated M012 pus swab innoculated in special Aerobic Transport 3.30 pm; Report AEROBIC, PUS SENSITIVITY if Identification & Medium available from LPL. Ship refrigerated. 3days indicated. Sensitivity

PAGE 100 OF 399 If AFB is suspected, use test code M050 CULTURE, AFB, Includes Conventional RAPID. If fungus is CULTURE, Sample Daily by ANTIBIOTIC Submit 5–10 mL (1 mL min.) sputum in a sterile Culture, Automated suspected, use test M011 AEROBIC, 3.30 pm; Report3 SENSITIVITY if screw capped container. Ship refrigerated. Identification & code M104 SPUTUM days indicated. Sensitivity CULTURE, FUNGUS, and/or M096 CULTURE, FUNGUS, NOCARDIA.

If N. gonorrhoeae is Includes Conventional CULTURE, Vaginal swab innoculated in Amie's charcoal suspected, usetest Sample Daily by ANTIBIOTIC Culture, Automated M016 AEROBIC, transport medium available from LPL. Ship code M018 3.30 pm; Report SENSITIVITY if Identification & VAGINAL SWAB refrigerated. CULTURE, 3days indicated. Sensitivity GONOCOCCUS.

PAGE 101 OF 399 Blood/Bone Marrow: Collect 8 mL (5 mL min.) Blood/Bone marrow in Myco F / Lytic bottle available from LPL. Mix by gentle inversion 8–10times. Ship at 18–22°C.CSF: Collect 2 mL (1 mL min.) CSF in a Red Top (No Additive) tube or sterile screw capped container. Do not use SST gel barrier tubes. Ship refrigerated. Pus/Body fluids (Pleural/ Pericardial /Ascitic/Synovial/Ocular) Aspirates/ Sample Daily by Semen /BAL / Bronchial washings: Submit as much Rapid Automated For AFB 3.30 pmReport Does not include as possible (1 mL min.) in sterile screw capped CULTURE, AFB Fluorescent AFB Antitubercular Interim: 14 days ANTI- container. Ship refrigerated.Endometrial (MYCOBACTERI Culture and Sensitivity, & 4 weeks M139 TUBERCULAR curettings/Tissue: Submit in sterile normal saline in UM), RAPID Automated AFB useappropriate test Final: 6 DRUG a sterile screw capped container. Ship WITH PNB TEST Identification by code. Request as weeksPNB test: SENSITIVITY. refrigerated.Swabs: Submit swabs in 1 mL sterile PNB test. additional tests. 10 days after AFB normal saline in sterile screw capped container. culture ispositive Ship refrigerated. Sputum/Urine: Submit 2 Spot (random) morning samples, 5–10 mL (1 mL min.) sputum / 10 mL (5 mL min.) urine in sterile screw capped container.Do not pool the samples. Ship refrigerated.Gastric Lavage: Submit 5–10 mL (2 mL min.) gastric lavage in a sterile screw capped container. Ship refrigerated . Overnight fasting is mandatory.

Submit as much as possible (1 mL min.) CSF OR Pus/Body Fluids Conventional Does not include Sample Daily by CULTURE, (Pleural/Pericardial/Ascitic/Synovial/Ocular)/ anaerobic culture M068 ANTIBIOTIC 3.30 pm;Report ANAEROBIC Aspirates OR Endometrial curettings/ Tissues OR and Automated SENSITIVITY. 8 days Bone Marrow in Anaerobic transport medium Identification available from LPL . Ship at room temperature.

PAGE 102 OF 399 This test includes CULTURE, Does not body fluid from Collect 8-10 mL body fluids aseptically in special Rapid Automated Sample Daily by ANAEROBIC, include sterile sites. This M134 Plus Anerobic bottle available from LPL. Mix by Anaerobic Culture 3.30 pm; Report BODY FLUID, ANTIBIOTIC test code does not gentle swirling. Ship at room temperature. and Identification 6days RAPID SENSITIVITY. include Urine /Pus specimens. 5 mL (1 mL min.) stool in a sterile screw capped Includes Conventional container within 6 hours. If there is a delay of more Sample Daily by CULTURE, ANTIBIOTIC Culture, Automated M076 than 6 hours collect specimen in special Stool 3.30 pm; Report CHOLERA SENSITIVITY if Identification & Culture Transport Medium available from LPL. Ship 3days indicated. Sensitivity refrigerated.DO NOT FREEZE Submit tissue in normal saline /catheter tip in sterile CULTURE, Includes screw capped container / 5 mL (1 mL min.) body Conventional Sample Daily by AEROBIC, ANTIBIOTIC effusion, aspirates, etc., in sterile screw capped Culture, Automated M020 3.30 pm; Report MISCELLANEOU SENSITIVITY if container OR swab in Aerobic Transport medium. Identification & 3days S indicated. Specify collection site on container and test request Sensitivity form. CULTURE, Does not include Collect 8-10 mL blood aseptically in special Plus Rapid Automated Also see CULTURE, Sample Daily by M021 ANAEROBIC, ANTIBIOTIC Anerobic bottle available from LPL. Mix by gentle Anaerobic Culture AEROBIC, 3.30 pm; Report BLOOD, RAPID SENSITIVITY swirling. Ship at room temperature. and Identification BLOOD,RAPID. 6days CULTURE ESBL (EXTENDED Rectal / Wound SWABS / Pure GROWTH in Amies Sample Daily by SPECTRUM M195 transport medium. Ship refrigerated only. DO NOT Chromogenic 11 am;Report BETA- FREEZE. Next day LACTAMASE), SCREENING Includes Sample Daily by CULTURE, Collect 8-10 mL whole blood / body fluids ANTIBIOTIC 3.30 pmReport FUNGUS, aseptically in a Myco / F-Lytic bottle available from Rapid Automated M024 SENSITIVITY for Interim: 2 days BLOOD / BODY LPL. Mix by gentle swirling. Ship at room Fungus Culture Yeast only if Final: 15 FLUIDS RAPID temperature. indicated. days

PAGE 103 OF 399 Submit Throat / Eye / Ear swab OR Sputum in Conventional Does not include Sample Daily by CULTURE, Amie'sTransport Medium available from LPL OR CSF culture and M095 ANTIBIOTIC 3.30 pm; Report HAEMOPHILUS in sterile screw capped container. Ship at room Automated SENSITIVITY. 3days temperature. identification CULTURE KPC Rectal / Wound SWABS / Pure GROWTH in Amies Sample Daily by M198 (CARBAPENEMA transport medium. Ship refrigerated only. DO NOT Chromogenic 11 amReport SE), SCREENING FREEZE. Next day

CULTURE MRSA (METHICILLIN Nasal / Axilla / Groin / Wound SWABS / Pure Sample Daily by RESISTANT M197 GROWTH in Amies transport medium. Ship Chromogenic 11 amReport STAPHYLOCOCC refrigerated only. DO NOT FREEZE. Next day US AUREUS), SCREENING

Sample Daily by Submit pus from discharging sinus / sputum in Does not include 3.30 pm;Report CULTURE, sterile screw capped container without fixatives OR M096 ANTIBIOTIC Conventional culture Interim : 1 week NOCARDIA tissue/ biopsy material / swabs in 1 mL sterile SENSITIVITY. Final: 3 normal saline. Ship refrigerated. weeks CULTURE, 180 mL potable water in (3 × 60 mL) sterile, screw WATER; Sample Daily by capped containers. Ship refrigerated. Specify water M035 PRESUMPTIVE Multiple tube test 3.30 pm; Report source on containers and test request form. Strictly COLIFORM 3days adhere to requested volumes. COUNT If C. diphtheriae is Includes Conventional CULTURE, suspected, usetest Sample Daily by ANTIBIOTIC Submit throat swab in Amie's transport medium Culture, Automated M009 AEROBIC, code M078 3.30 pm; Report SENSITIVITY if available from LPL. Ship refrigerated. Identification & THROAT SWAB CULTURE, 3days indicated. Sensitivity DIPHTHERIA.

PAGE 104 OF 399 CULTURED Sub-culture, Stability of the AEROBIC Submit pure isolate on appropriate media. Ship Sample Daily by Automated isolate will be M141 BACTERIAIDENT refrigerated. DO NOT FREEZE. Specify collection site 3.30 pm; Report identification and determined by the IFICATION AND and provide brief clinical history. 3days Sensitivity laboratory. SENSITIVITY

Mixed bacteria CULTURED culture sensitivity or Submit pure known isolate on appropriate media. Sub-culture and Sample Daily by AEROBIC fungus sensitivity or M140 Ship refrigerated. DO NOT FREEZE. Specify collection Automated 3.30 pm; Report BACTERIASENSI AFB sensitivity are site and name of the isolate grown. Sensitivity Nextday TIVITY not included in this test. Specimens submitted in the *Amikacin laboratory for AFB *Kanamycin Submit pure growth of Mycobacterium tuberculosis culture are CULTURED AFB *Capreomycin complex on appropriate media in sterile screw preserved for a ANTITUBERCUL *Clofazimine capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by AR DST, RAPID *Ethionamide refrigerated OR when used with other AFB culture Automated the culture is M182 3.30 pm; Report 10 SECOND *Ofloxacin * or Mycobacteria identification tests the pure Fluorescent positive. If 21days LINE DRUGS Levofloxacin * growth obtained in the Laboratory from specimens Antitubercular PANEL Moxifloxacin already submitted will be processed for sensitivity is *PAS * Antitubercular Sensitivity. required on this Cycloserine sample, kindly order the test within 15 days

PAGE 105 OF 399 Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB *Ofloxacin capped container in a sealed plastic bag. Ship period of 15 days if ANTITUBERCUL *Levofloxacin Sample Daily by refrigerated OR when used with other AFB culture Automated the culture is M183 AR DST, RAPID *Moxifloxacin 3.30 pm; Report or Mycobacteria identification tests, the pure Fluorescent positive. If 5 SECOND LINE *PAS 21days growth obtained in the Laboratory from specimens Antitubercular DRUGS PANEL *Cycloserine already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days Specimens submitted in the laboratory for AFB culture are Submit pure growth of MOTT on appropriate media CULTURED AFB preserved for a in sterile screw capped container in a sealed plastic OTHER THAN period of 15 days if bag. Ship refrigerated OR when used with other AFB Sample Daily by TUBERCULOSIS Broth Microdilution the culture is M181 culture or Mycobacteria identification tests the 3.30 pm; Report (MOTT), DST (MIC) positive. If pure growth (MOTT) obtained in the Laboratory 7days FOR RAPID Antitubercular specimens already submitted will be processed for GROWERS sensitivity is MOTT Drug Sensitivity. required on this sample, kindly order the test within 15 days Includes Conventional CULTURE, Sample Daily by ANTIBIOTIC Submit semen in a sterile screw capped container. Culture, Automated M013 AEROBIC, 3.30 pm; Report SENSITIVITY if Ship refrigerated. Identification & SEMEN 3days indicated. Sensitivity

PAGE 106 OF 399 Blood/Bone Marrow: Collect 8 mL (5 mL min.) Blood/Bone marrow in Myco F / Lytic bottle available from LPL. Mix by gentle inversion 8–10 times. Ship at 18–22°C.CSF: Collect 2 mL (1 mL min.) CSF in a Red Top (No Additive) tube or sterile screw capped container. Do not use SST gel barrier tubes. Ship refrigerated.Pus/Body fluids Sample Daily by (Pleural/Pericardial/Ascitic/ Synovial/Ocular) 3.30 pmReport Aspirates /Semen/ BAL Bronchial washings: Submit For AFB Interim: 14 days Does not include as much as possible (1 mL min.) in sterile screw Antitubercular & 4 weeks CULTURE, AFB ANTI- capped container. Ship refrigerated.Endometrial Automated Sensitivity, use Final: 6 M050 (MYCOBACTERI TUBERCULAR curettings/Tissue: Submit in sterile normal saline in Fluorescent, ICT, appropriate test weeksPositive UM), RAPID DRUG a sterile screw capped container. Ship MALDI-TOF code. Request as results between SENSITIVITY. refrigerated.Swabs: Submit swabs in 1 mL sterile additional tests. 2-42 daysshall be normal saline in sterile screw capped container. informed Ship refrigerated.Sputum/Urine: Submit 2 Spot telephonically. (random) morning samples, 5-10 mL (1 mL min.) sputum/10 mL (5 mL min.) urine in sterile screw capped container. Do not pool the samples.Ship refrigerated.Gastric Lavage: Submit 5-10 mL (2 mL min.) gastric lavage in a sterile screw capped container. Ship refrigerated . Overnight fasting is mandatory.

Does not Conventional CULTURE, Also see C. DIFFICILE Sample Daily by include 5 g (1 g min.) of fresh stool in Anaerobic transport anaerobic culture M070 CLOSTRIDIUM TOXIN A andB, 3.30 pm; Report ANTIBIOTIC medium available from LPL. Ship refrigerated. and Automated DIFFICILE STOOL. 8days SENSITIVITY. Identification

PAGE 107 OF 399 CSF: 2 mL (1 mL min.) in a Red Top (No Additive) tube or sterile screw capped vial. Do not use SST gel barrier tubes. Ship refrigerated.Bone Marrow: 2 mL (0.5 mL min.) Bonemarrow in 1 Green Top (Sodium Heparin) tube. Ship refrigerated.Pus/Body fluids (Pleural/ Pericardial/ Ascitic/ Synovial/ Ocular/ Bronchial washings/BAL). Aspirates /Semen/ Stool: Submit as much as possible (1 mL/1g min.) in sterile screw capped container. Ship refrigerated. Sample Daily by Endometrial curettings/Tissue: Submitin sterile 3.30 pm;Report normal saline in a sterile screw capped container. Contact lab within Interim 1: 1 week Does not include Ship refrigerated.Swabs: Submit swabs in 1 mL 10 days of release of CULTURE, Conventional Interim M104 ANTIFUNGAL sterile normal saline in sterile screw capped result if Antifungal FUNGUS Fungus Culture 2: 2 weeks SENSITIVITY. container. Ship refrigerated. Sputum/Urine: Submit drug sensitivity is Interim 3: 3 2 Spot (random) morning samples, 5–10 mL (1 mL required. weeks min.) sputum/10 mL (5 mL min.) urine in sterile Final: 4 weeks screw capped container. Shiprefrigerated.Skin: Submit 2–3 mm scrapings from periphery of active skin lesion in sterile container or between 2 sterile glass slides. Wrap slides in black paper. Ship refrigerated.Hair: Submit plucked 10–12 infected, lustreless hair including root in a sterile container. Ship refrigerated.Nail: Submit clippings and powdery material from friable and discolored region of affected nail. Ship refrigerated.

Also see C. DIFFICILE Includes Conventional Submit 2–5 g (1 g min.) stool in a Special Stool TOXIN A and B and Sample Daily by CULTURE, ANTIBIOTIC Culture, Automated M008 Culture Transport Medium available from LPL. Ship CULTURE, 3.30 pm; Report STOOL SENSITIVITY if Identification & refrigerated. CLOSTRIDIUM 3days indicated. Sensitivity DIFFICILE.

PAGE 108 OF 399 Mixed bacteria culture identification or fungus identification or AFB identification are CULTURED not included in this Does not include Submit pure isolate on appropriate media. Ship Sub-culture and Sample Daily by AEROBIC test. For ANTIBIOTIC M069 ANTIBIOTIC refrigerated. DO NOT FREEZE. Specify collection site Automated 3.30 pm; Report BACTERIAIDENT SENSITIVITY, use SENSITIVITY. and provide brief clinical history. identification Nextday IFICATION test code M140 CULTURED, AEROBIC, BACTERIA SENSITIVITY. Request as additional test.

Specimens submitted in the laboratory for AFB culture are Submit pure growth of MOTT on appropriate media CULTURED AFB preserved for a in sterile screw capped container in a sealed plastic OTHER THAN period of 15 days if bag. Ship refrigerated OR when used with other Sample Daily by TUBERCULOSIS Broth Microdilution the culture is M180 AFB culture or Mycobacteria identification tests the 3.30 pm; Report (MOTT), DST (MIC) positive. If pure growth (MOTT) obtained in the Laboratory 14days FOR SLOW Antitubercular specimens already submitted will be processed for GROWERS sensitivity is MOTT Drug Sensitivity. required on this sample, kindly order the test within 15 days

PAGE 109 OF 399 CULTURED Does not include Conventional fungus Sample Daily by Submit pure isolate on appropriate medium. Ship M093 FUNGUS ANTIFUNGAL culture and 3.30 pm; Report refrigerated. DO NOT FREEZE. IDENTIFICATION SENSITIVITY identification 3–28days Streptomycin, Specimens Isoniazid, submitted in the Rifampicin, laboratory for AFB Ethambutol Submit pure growth of Mycobacterium tuberculosis culture are Pyrazinamide complex on appropriate media in sterile screw preserved for a CULTURED AFB Clofazimine capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL Ethionamide refrigerated OR when used with other AFB culture Automated the culture is M176 3.30 pm; Report AR DST, 15 Capreomycin or Mycobacteria identification tests, the pure Fluorescent positive. If 21days DRUGS PANEL Kanamycin growth obtained in the Laboratory from specimens Antitubercular Amikacin already submitted will be processed for sensitivity is Ofloxacin +PAS Antitubercular Sensitivity. required on this Cycloserine sample, kindly order Levofloxacin the test within 15 Moxifloxacin days Streptomycin, Specimens Isoniazid, submitted in the Rifampicin, laboratory for AFB Ethambutol Submit pure growth of Mycobacterium tuberculosis culture are Pyrazinamide complex on appropriate media in sterile screw preserved for a Clofazimine CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Ethionamide Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M224 Capreomycin 3.30 pm; Report AR DST, 16 or Mycobacteria identification tests, the pure Fluorescent positive. If Kanamycin 21days DRUGS PANEL growth obtained in the Laboratory from specimens Antitubercular Amikacin already submitted will be processed for sensitivity is Ofloxacin +PAS Antitubercular Sensitivity. required on this Cycloserine sample, kindly order Levofloxacin the test within 15 Moxifloxacin * days Linezolid

PAGE 110 OF 399 Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M109 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days STREPTOMYCIN growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M110 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days ISONIAZID growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days

PAGE 111 OF 399 Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M177 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days CYCLOSERINE growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M115 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days ETHIONAMIDE growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days CULTURED Does not include Submit pure isolate on appropriate medium. Ship Sub-Culture and Sample Daily by ANAEROBIC M092 ANTIBIOTIC refrigerated. DO NOT FREEZE. Specify collection site Automated 3.30 pm; Report BACTERIAIDENT SENSITIVITY. and provide brief clinical history. Identification 8days IFICATION

PAGE 112 OF 399 Specimens submitted in the laboratory for AFB Streptomycin,Iso Submit pure growth of Mycobacterium tuberculosis culture are niazid,Rifampicin, complex on appropriate media in sterile screw preserved for a Ethambutol CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Pyrazinamide Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M107 +Clofazimine 3.30 pm; Report AR DST, 10 or Mycobacteria identification tests, the pure Fluorescent positive. If Ethionamide 21days DRUGS PANEL growth obtained in the Laboratory from specimens Antitubercular Capreomycin already submitted will be processed for sensitivity is Kanamycin Antitubercular Sensitivity. required on this Amikacin sample, kindly order the test within 15 days Specimens submitted in the Streptomycin, laboratory for AFB Isoniazid, Submit pure growth of Mycobacterium tuberculosis culture are Rifampicin, complex on appropriate media in sterile screw preserved for a Ethambutol CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Pyrazinamide Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M123 Clofazimine 3.30 pm; Report AR DST, 11 or Mycobacteria identification tests, the pure Fluorescent positive. If Ethionamide 21days DRUGS PANEL growth obtained in the Laboratory from specimens Antitubercular Capreomycin already submitted will be processed for sensitivity is Kanamycin Antitubercular Sensitivity. required on this Amikacin sample, kindly order +Ofloxacin the test within 15 days

PAGE 113 OF 399 Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Streptomycin,Iso Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M105 niazid,Rifampicin, 3.30 pm; Report AR DST, 4 or Mycobacteria identification tests, the pure Fluorescent positive. If Ethambutol 21days DRUGS PANEL growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB Streptomycin,Iso capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL niazid,Rifampicin, refrigerated OR when used with other AFB culture Automated the culture is M106 3.30 pm; Report AR DST, 5 Ethambutol + or Mycobacteria identification tests, the pure Fluorescent positive. If 21days DRUGS PANEL Pyrazinamide growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days

PAGE 114 OF 399 Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M225 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days LINEZOLID growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M117 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days CAPREOMYCIN growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days

PAGE 115 OF 399 Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M114 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days AMIKACIN growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a capped container in a sealed plastic bag. Ship period of 15 days if CULTURED AFB Sample Daily by refrigerated OR when used with other AFB culture Automated the culture is M120 ANTITUBERCUL 3.30 pm; Report or Mycobacteria identification tests, the pure Fluorescent positive. If AR DST, PAS 21days growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days

PAGE 116 OF 399 Submit pure growth of Mycobacterium tuberculosis complex on appropriate media in sterile screw CULTURED AFB capped container in a sealed plastic bag. Ship Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated M111 u 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent 21days RIFAMPICIN growth obtained in the Laboratory from specimens already submitted will be processed for Antitubercular Sensitivity. Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M112 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days ETHAMBUTOL growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days

PAGE 117 OF 399 Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M113 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days KANAMYCIN growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M119 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days OFLOXACIN growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days

PAGE 118 OF 399 Specimens submitted in the Streptomycin, laboratory for AFB Isoniazid, Submit pure growth of Mycobacterium tuberculosis culture are Rifampicin, complex on appropriate media in sterile screw preserved for a Ethambutol CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Pyrazinamide Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M124 Clofazimine 3.30 pm; Report AR DST, 12 or Mycobacteria identification tests, the pure Fluorescent positive. If Ethionamide 21days DRUGS PANEL growth obtained in the Laboratory from specimens Antitubercular Capreomycin already submitted will be processed for sensitivity is Kanamycin Antitubercular Sensitivity. required on this Amikacin sample, kindly order +Ofloxacin + PAS the test within 15 days Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M108 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days PYRAZINAMIDE growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days

PAGE 119 OF 399 Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M179 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days MOXIFLOXACIN growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M178 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days LEVOFLOXACIN growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days

PAGE 120 OF 399 Specimens submitted in the laboratory for AFB Submit pure growth of Mycobacterium tuberculosis culture are complex on appropriate media in sterile screw preserved for a CULTURED AFB capped container in a sealed plastic bag. Ship period of 15 days if Sample Daily by ANTITUBERCUL refrigerated OR when used with other AFB culture Automated the culture is M118 3.30 pm; Report AR DST, or Mycobacteria identification tests, the pure Fluorescent positive. If 21days CLOFAZIMINE growth obtained in the Laboratory from specimens Antitubercular already submitted will be processed for sensitivity is Antitubercular Sensitivity. required on this sample, kindly order the test within 15 days CULTURED MOLD Submit Pure GROWTH of Mold on appropriate ANTIFUNGAL Sample Daily by 9 M223 media in sterile screw capped container. Ship Micro-broth DRUG am; Report 7days refrigerated. DO NOT FREEZE. SENSITIVITY (DST) TEST 3 mL (2 mL min.) Whole blood in 1 Lavender Top Sample Daily by 5 CYCLOSPORINE (EDTA) tube. Ship refrigerated. Ideal Sampling Time: G159 LC-MS/MS pm; Report Same A Just before the next dose (Trough levels). Provide day date and time of sampling, amount & time of dose. Sample by Sat 11 CYP3A4*22 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS126 PCR, Sequencing am; Report 10 GENOTYPING (EDTA) tube. Ship refrigerated. DO NOT FREEZE. days Sample by Wed CYP2C19 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS125 PCR, Sequencing 11 am; Report GENOTYPING (EDTA) tube. Ship refrigerated. DO NOT FREEZE. 11 days

PAGE 121 OF 399 Sample by Mon / 2 mL (1 mL min.) serum from 1 SST. Ship B173 CYSTATIN C Nephelometry Wed / Fri 4 pm; refrigerated or frozen. Report Same day

CYSTICERCOSIS Sample by Mon 9 (TAENIA 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S033 am; Report Same SOLIUM) refrigerated or frozen. Immunoassay day ANTIBODY, IgG CYSTIC 4 mL (2 mL min.) Whole Blood in 1 Lavender top Sample Daily by FIBROSIS (EDTA) tube OR 10 mL Amniotic fluid in a sterile OS127 PCR, Sequencing 11 am; Report MUTATION screw capped container. Ship refrigerated. DO NOT 13 days DETECTION FREEZE. CYSTIC 1 drop of heel prick blood each on 3 spots of filter Sample Tue / Thu FIBROSIS, paper available from LPL. Clinical details and drug R162 Fluoroimmunoassay / Sat by 5 pm; NEWBORN, history must accompany sample. Ship refrigerated Report 2 days SCREEN or frozen CYTOLOGY Collect smears onto 2 glass slides and fix (PAP SMEAR), immediately in Absolute alcohol for 30 min and air Microscopy of Sample Daily by 6 Do not take smears J006 GENITAL, dry. Do not allow smears to air dry before fixation. Papanicolaou pm; Report Next during menstruation. FEMALE, Brief clinical history in Cytology Requisition Form is stained smears day CONVENTIONAL mandatory. Collect Endocervical smears with a brush after CYTOLOGY inserting the speculum. Dip & swirl the brush (PAP SMEAR), completely into the container with liquid fixative. Microscopy of Sample Daily by 6 Do not take smears J152 GENITAL, Mix thoroughly. Use Special Collection Kit available Papanicolaou pm; Report Next during menstruation. FEMALE, from LPL only. Ship at room temperature. Brief stained smears day LIQUID BASED clinical history in Cytology Requisition Form is mandatory.

PAGE 122 OF 399 Submit Body fluids (Ascitic / Pleural / Pericardial / CSF/ Synovial / Prostatic / Urethral) OR Bronchial washings / Lavage OR Sputum OR second morning Urine specimen OR Nipple discharge in a sterile CYTOLOGY screw capped container. Mix with equal quantities Microscopy of Sample Daily by 6 J011 EXAMINATION, of 50% alcohol. Alternatively centrifuge, make Papanicoloau / pm; Report Next BODY FLUIDS smears from the deposit and fix in Absolute alcohol MGG stained smears day for 30 minutes and air dry. Do not allow smears to air dry before fixation. Specimens in syringes are not acceptable. Brief clinical history in Cytology Requisition Form is mandatory.

Submit cytopathological material in a screw capped CYTOLOGY, Sample Daily by 6 container and fix immediately in equal quantity of J074 CELL BLOCK Cell block pm; Report 3 50% alcohol for 30 min. Give brief clinical history on EXAMINATION days Cytopathology Test Requisition form.

Aspirate material from Breast / Superficial lumps / CT or USG guided deep lumps. Smear on 4 glass CYTOLOGY, slides and fix immediately in Absolute alcohol for 30 Microscopy of Sample Daily by 6 FINE NEEDLE J004 minutes. Air dry completely. Do not allow smears Papanicoloau / pm; Report Next ASPIRATION; to air dry before fixation. Specimens in syringes are MGG stained smears day FNAC not acceptable. Brief clinical history in Cytology Requisition Form is mandatory. CYTOLOGY Submit cytopathological slides previously reported Sample Daily by 6 CONSULTATION upon. Previous report(s), clinical history to be J008 Microscopy pm; Report Next , SECOND submitted with the Cytopathology Test Requisition day OPINION form.

PAGE 123 OF 399 CYTOMEGALOVI RUS (CMV) 2 mL (0.5 mL min.) serum from 1 SST. Ship Z122 ANTIBODIES CLIA Daily refrigerated or frozen. PANEL, IgG & IgM CYTOMEGALOVI 2 mL (0.5 mL min.) serum from 1 SST. Ship S041 RUS (CMV) CLIA Daily refrigerated or frozen. ANTIBODY, IgG

CYTOMEGALOVI 2 mL (0.5 mL min.) serum from 1 SST. Ship S022 RUS (CMV) CLIA Daily refrigerated or frozen. ANTIBODY, IgM CYTOMEGALOVI Sample Daily by 9 2 mL (0.5 mL min.) serum from 1 SST. Ship Enzyme B156 RUS (CMV) am; Report Same refrigerated or frozen. Immunoassay AVIDITY, IgG Day

5 mL (3 mL min.) Amniotic fluid in a sterile screw capped container collected after 21 weeks of pregnancy for Prenatal testing OR 10 mL(5 mL min) Urine/Saliva in a sterile screw capped container OR 2 mL(1 mL min) Plasma from 1 Lavendar top (EDTA) CYTOMEGALOVI tube OR 2 mL(1 mL min) Serum from 1 Red top (No Sample by Mon / RUS (CMV), N017 additive) tube OR 2 mL(1 mL min) CSF/BAL fluid in a Real time PCR Thu 11 am; QUALITATIVE sterile screw capped conmtainer OR 5 g (min 3 g) ReportWed / Sat PCR Fresh Tissue in normal saline.Ship refrigerated. Separate serum / plasma aseptically within 2 hours of collection. 12 hours overnight fasting is recommended. Brief clinical history and nature of specimen on Test Request Form is mandatory.

PAGE 124 OF 399 CYTOMEGALOVI 2 mL (1 mL min.) plasma from 1 Lavender Top ( Sample by Mon / RUS (CMV), EDTA) tube. Separate plasma aseptically within 6 N031 Real Time PCR Thu 11 am; DNA,QUANTITA hours of collection. Ship refrigerated or frozen. 12 ReportWed / Sat TIVE, PCR hours overnight fasting is recommended.

3 mL Whole blood in 1 Blue Top (Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hrs. If this is not possible, make PPP within 1 hour of collection as follows: Centrifuge sample at Also see DEEP VEIN 3600 rpm for 15 min & transfer supernatent to a THROMBOSIS PANEL D – DIMER, clean plastic tube. Centrifuge this supernatant again H134 Immunoturbidimetry & FIBRINOGEN Daily QUANTITATIVE at 3600 rpm for 15 mins & finally transfer the DEGRADATION supernatent (PPP) to one labelled clean plastic PRODUCTS. screw capped vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form is mandatory.

It is recommended 2 mL (1 mL min.) serum from 1 SST AND 6 mL that patient *Antithrombin Whole blood in 2 Blue Top (Sodium Citrate) tubes. discontinues Activity Mix thoroughly by inversion. Transport to Lab Heparin for 1 day Functional within 4 hrs. If this is not possible make PPP within 1 andOral DEEP VEIN *CardiolipinAntib hour of collection as follows: Centrifuge sample at Anticoagulants for 7 Electromechanical & THROMBOSIS odies IgG and 3600 rpm for 15 min & transfer supernatent to a days prior to Sample by Wed 7 Z056 Photo-optical Clot PANEL; DVT IgM *dRVVT clean plastic tube. Centrifuge this supernatant again sampling as these am; Report Fri Detection, EIA PANEL *Protein C at 3600 rpm for 15 mins & finally transfer the drugs may affect Functional supernatant (PPP) to 1 labelled, clean plastic screw test results. *Protein S capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation Functional NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician

PAGE 125 OF 399 Recommended test is NS1 Antigen by ELISA in the first 5 DENGUE FEVER days of fever. After Sample Daily by 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z674 ANTIBODIES 7-10 days of fever, 12 noon; Report refrigerated or frozen. Immunoassay IgG & IgM, EIA the recommended same day test is Dengue feverantibodies IgG & IgM by ELISA.

Recommended test is NS1 Antigen by ELISA in the first 5 DENGUE FEVER days of fever. After ANTIBODIES 2 mL (1 mL min.) serum from 1 SST. Ship S151 ICT 7-10 days of fever, Daily PANEL, IgG & refrigerated or frozen. the recommended IgM test is Dengue fever antibodies IgG & IgM by ELISA.

Recommended test is NS1 Antigen by *Dengue fever ELISA in the first 5 DENGUE FEVER antibodies IgG & ICT, Lateral Flow days of fever. After 2 mL (1 mL min.) serum from 1 SST. Ship Z510 COMBINED IgM *Dengue Immuno- 7-10 days of fever, Daily refrigerated or frozen. PANEL fever NS1 chromatography the recommended antigen test is Dengue feverantibodies IgG & IgM by ELISA.

PAGE 126 OF 399 Recommended test is NS1 Antigen by ELISA in the first 5 DENGUE FEVER days of fever. After Sample Daily by 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S217 IgG ANTIBODY, 7-10 days of fever, 12 noon; Report refrigerated or frozen. Immunoassay EIA the recommended same day test is Dengue feverantibodies IgG & IgM by ELISA.

Recommended test is NS1 Antigen by ELISA in the first 5 DENGUE FEVER 2 mL (1 mL min.) serum from 1 SST OR 2 mL (1 mL Lateral Flow days of fever. After H063 NS1 ANTIGEN, min.) plasma from 1 Lavender Top (EDTA) tube. Ship Immunochromatogr 7-10 days of fever, Daily RAPID refrigerated or frozen. aphy the recommended test is Dengue feverantibodies IgG & IgM by ELISA.

DENGUE FEVER Sample Daily by :2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S225 NS1 ANTIGEN, 12 noon; Report refrigerated or frozen. Immunoassay EIA same day

PAGE 127 OF 399 Recommended test is NS1 Antigen by ELISA in the first 5 DENGUE FEVER days of fever. After Sample Daily by 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S216 IgM ANTIBODY, 7-10 days of fever, 12 noon; Report refrigerated or frozen. Immunoassay EIA the recommended same day test is Dengue feverantibodies IgG & IgM by ELISA.

3 mL (2 mL min.) Whole Blood / Plasma in 1 DENGUE & Lavender Top (EDTA) Tube OR 2 mL (1 mL min.) Sample Daily by CHIKUNGUNYA Z853 Serum from 1 SST OR 2 mL (1 mL min.) CSF in a Real Time PCR 11 am; Report VIRUSES, PCR sterile screw capped container. Ship refrigerated. Next day QUALITATIVE DO NOT FREEZE 3 mL (2 mL min.) Whole Blood / Plasma in 1 DENGUE VIRUS, Lavender Top (EDTA) Tube OR 2 mL (1 mL min.) Sample Daily by N132 PCR Serum from 1 SST OR 2 mL (1 mL min.) CSF in a Real Time PCR NA 11 am; Report QUALITATIVE sterile screw capped container. Ship refrigerated. Next day DO NOT FREEZE Sample by 7th of 3 mL (1.5 mL min.) serum from 1 Red Top (No DEOXYCORTICO the month; RW058 Additive) tube. Separate serum from cells LC-MS/MS STERONE Reportafter 2–3 immediately. Ship refrigerated or frozen. weeks 4 mL (2 mL min.) serum from 1 Red Top (No Sample by 7th of Additive) tube. Separate serum and transfer into the month; RW059 DESIPRAMINE screw capped vial. Do not use SST gel barrier tubes. LC-MS/MS Reportafter 2–3 Ship refrigerated or frozen. Recommended weeks specimen: >12 hours post oral dose. DESMOGLEIN Sample by Mon 9 2 mL (1 mL min.) serum from 1 SST. Ship S099 (DSG) 1 AND 3 IFA am; Report Wed refrigerated or frozen. Overnight fasting is preferred. ANTIBODY 6 pm

PAGE 128 OF 399 2 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th of DEXAMETHASO Additive) tube. Do not use SST gel barrier tubes. the month; RW060 LC-MS/MS NE Draw specimen between 8–10 A.M. Ship Reportafter 2–3 refrigerated or frozen. weeks DHEA; 2 mL (0.5 mL min.) serum from 1 SST. Overnight Also see DHEA Sample by Tue 9 Enzyme R100 DEHYDROEPIAN fasting is preferred. Specify age and sex on test STIMULATION am; Report Same Immunoassay DROSTERONE request form. Ship refrigerated or frozen. BYACTH. day DHEA SULPHATE; 2 mL (0.5 mL min.) serum from 1 SST. Overnight Chemiluminescent R044 DEHYDROEPIAN fasting is preferred. Specify age and sex on test MicroparticleImmun Daily DROSTERONE request form. Ship refrigerated or frozen. oassay SULPHATE 2 mL (0.5 mL min.) serum from 1 SST for each timed Synthetic ACTH specimen. Draw fasting baseline sample; administer (Synacthen) is not DHEA 250 ug of synthetic ACTH (Synacthen) IV, draw supplied by LPL. Sample by Tue 9 Enzyme Z022 STIMULATION additional sample at 90 min. Ship refrigerated or Refer to Y CODES am; Report Same Immunoassay BY ACTH frozen. Both tubes must be clearly marked with Y046 BasalY047 day time drawn. Submit both tubes with 1 test request 90 min.Y049 form. Customized 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium DIABETES *Glucose F / PP / Fluoride) tube AND 3 mL (1.5 mL min.) whole Z312 Hexokinase, HPLC Daily PANEL BASIC R *HbA1c blood in 1 Lavender Top (EDTA) tube. Ship refrigerated. DO NOT FREEZE. 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium DIABETES *Glucose F + PP Fluoride) tube each AND 10 mL (5 mL min.) aliquot Z359 Hexokinase, Dipstick Daily SCREEN *Urine Glucose of first morning urine. Ship refrigerated. DO NOT FREEZE. Overnight fasting is mandatory.

PAGE 129 OF 399 2 mL (1 mL min.) plasma each from 2 Grey Top *Glucose F + PP (Sodium Fluoride) tubes for Glucose F + PP AND 3 *Cholesterol * DIABETES mL (1.5 mL min.) serum from 1 SST AND 3 mL (2 Spectrophotometry, Z285 Triglycerides*Hb Daily PANEL 1 mL min.) whole blood from 1 Lavender Top (EDTA) HPLC A1c *Urea tube. Ship refrigerated. DO NOT FREEZE. Minimum *Creatinine 12 hours fasting is mandatory.

*Glucose F + PP *HbA1c *Uric 2 mL (1 mL min.) plasma each from 2 Grey Top Acid *Creatinine (Sodium Fluoride) tubes for Glucose F + PP AND 3 *Cholesterol mL (2 mL min.) serum from 1 SST AND 3 mL (2 mL DIABETES Spectrophotometry, Z286 *HDL *LDL min.) whole blood from 1 Lavender Top (EDTA) tube Daily PANEL 2 HPLC, Dipstick Calculated AND 10 mL (5 mL min.) aliquot of first morning *Triglyceride urine Ship refrigerated. DO NOT FREEZE. 12 hours *Microalbumin strict overnight fasting is mandatory. *Urine R/E *GAD-65* Anti- DIABETES TYPE IA2* Insulin Sample by Fri 9 1, 5 mL (3 mL min.) serum from 1 SST. Ship Enzyme Z833 Autoantibody* am; Report Next AUTOIMMUNE refrigerated or frozen. Immunoassay, IFA Islet Cell day PROFILE Antibody 3 mL (2 mL min.) serum from 1 Red Top (No Sample by 7th of Includes Additive) tube. Do not use SST gel barrier tubes. the month; RW061 DIAZEPAM metabolite Ship refrigerated or frozen. Recommended LC-MS/MS Reportafter 2–3 Nordiazepam. specimen: just before the next dose is due at steady weeks state. 2 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th of Additive) tube. Do not use SST gel barrier tubes. the month; RW062 DIGITOXIN Separate serum from cells immediately. Ship Immunoassay Reportafter 2–3 refrigerated or frozen. Ideal sampling time: just weeks before the next dose is due at steady state.

PAGE 130 OF 399 2 mL (1 mL min.) serum from 1 Red Top (No Additive) tube. Do not use SST gel barrier tubes. Separate serum within 2 hours of collection. Ship Chemiluminescent D008 DIGOXIN Daily refrigerated or frozen. Trough: just before the next Immunoassay dose is due at steady state. Peak : 6–8 hours after dose. 5α- Sample by Thu 9 DIHYDROTESTO 2 mL (1 mL min.) serum from 1 SST. Specify age and Enzyme R103 am; Report Same STERONE; 5α- sex on test request form. Ship refrigerated or frozen. Immunoassay Day DHT Sample by 7th of DIHYDROTESTO 5 mL (2.5 mL min.) serum from 2 SST's. Ship the month; RW063 STERONE LC-MS / MS refrigerated or frozen. reportafter 2–3 (DHT), FREE weeks. 2 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th of Additive) tube. Separate serum from cells the month; RW064 DISOPYRAMIDE Immunoassay immediately. Do not use SST gel barrier tubes. Ship Reportafter 2–3 refrigerated or frozen. weeks

3 mL (1 ml min.) whole blood in 1 Lavender Top It is recommended (EDTA) tube. Ship refrigerated. DO NOT FREEZE. that patient AND 6 mL Whole blood in 2 Blue Top (Sodium discontinues Citrate) tubes. Mix thoroughly by inversion. Heparin for 1 day Transport to Lab within 4 hrs. If this is not possible, and Oral DISSEMINATED *CBC make PPP within 1 hour of collection as follows: Anticoagulants for 7 INTRAVASCULA *Prothrombin Photo-optical Clot Centrifuge sample at 3600 rpm for 15 min & days prior to H245 R studies *APTT Detection,Electrical Daily transfer supernatent to a clean plastic tube. sampling as these COAGULATION *Fibrinogen* D- Impedence Centrifuge this supernatant again at 3600 rpm for drugs may affect (DIC) PROFILE Dimer 15 mins & finally transfer the supernatant (PPP) to 1 test results. labelled, clean plastic screw capped vial. FREEZE Discontinuation IMMEDIATELY. Ship frozen. DO NOT THAW. should be with prior Overnight fasting is preferred. Duly filled consent from the Coagulation Requisition Form is mandatory. treating Physician.

PAGE 131 OF 399 DLC (DIFFERENTIAL Electronic Cell 3 mL (1 mL min.) whole blood in 1 Lavender Top Also see ABSOLUTE H023 LEUCOCYTE Cytometry, VCS Daily (EDTA) tube. Ship refrigerated. DO NOT FREEZE. COUNTS. COUNT), &Microscopy AUTOMATED DLC ( DIFFERENTIAL 2 mL (1 mL min.) fluid in a sterile screw capped P084 Manual count Daily LEUCOCYTE container. Ship refrigerated. DO NOT FREEZE. COUNT), FLUID

3 mL (2 mL min.) whole blood from 1 Lavender Top DNA ANALYSIS (EDTA) tube for each Parent and Child. Ship Sample Daily by For 2 individuals N107 FOR KINSHIP refrigerated. DO NOT FREEZE. Paternity Consent PCR, STR analysis 11 am; Report 10 price is Rs.10,000/- TESTING form with photograph and identification document days for each individual is mandatory.

3 mL (2 mL min.) whole blood from 1 Lavender Top DNA ANALYSIS (EDTA) tube for each Parent and Child. Ship Sample Daily by For 2 individuals N106 FOR PATERNITY refrigerated. DO NOT FREEZE. Paternity Consent PCR, STR analysis 11 am; Report 10 price is Rs.10,000/- TESTING form with photograph and identification document days for each individual is mandatory.

3 mL (2 mL min.) Whole blood from 1 Lavender Top DNA ANALYSIS (EDTA) tube for each individual. Ship refrigerated. Sample Daily by FOR PATERNAL For 2 individuals N115 DO NOT FREEZE. Paternity Consent form with PCR, STR analysis 11 am; Report 12 Y price is Rs.20,000/- photograph and identification document for each days CHROMOSOME individual is mandatory. Sample Daily by 5 10 mL ( 5 mL min.) aliquot of first morning urine OR G140 DNPH, URINE Biochemical pm; Report Same post feed urine. Ship refrigerated or frozen. Day

PAGE 132 OF 399 3 mL (1.5 mL min.) serum from 1 Red Top (No Sample by 7th of Includes Additive) tube. Do not use SST gel barrier tubes. the month; RW065 DOXEPIN metabolite LC-MS/MS Ship refrigerated or frozen. Recommended Reportafter 2–3 Nordoxepin. specimen: >12 hours post oral dose. weeks DPD Gene Sample by Sat 9 Mutations (5- 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS130 PCR am; Report 10 FU toxicity) (EDTA) tube. Ship refrigerated. DO NOT FREEZE. days detection Also see ALKALINE PHOSPHATASE ISOENZYMES; ALKALINE PHOSPHATASE, BONE SPECIFIC; COLLAGEN CROSS- LINKED N- DPYD 20 ml (10 mL min.) aliquot of first morning urine. Sample by Mon 4 Chemiluminescent TELOPEPTIDE; R122 (DEOXYPYRIDIN Ship refrigerated or frozen. Wrap the specimen in pm; Report Same Immunoassay OSTEOPOROSIS OLINE), URINE aluminium foil to protect from light. Day PANEL. Deoxypyridinoline, when used for monitoring therapy, should be collected at the same time on each occasion as for the Ç Sample by Tue 11 DRPLA GENE 4 mL (2 mL min.) Whole Blood in 1 Lavender top PCR Fragment OS132 am; Report 10 ANALYSIS (EDTA) tube. Ship refrigerated. DO NOT FREEZE. analysis days

PAGE 133 OF 399 * Opiates *Benzodiazepine *Cannabinoids*C DRUGS OF ocaine & 10 mL (5 mL min.) aliquot of random urine in a ABUSE, 7 Lateral Flow V617 metabolites sterile screw capped container. Ship refrigerated or Daily DRUGS URINE Immunoassay *Amphetamine* frozen. SCREEN Barbiturate * Phencyclidine (PCP)

DUCHENNE / BECKER MUSCULAR Sample by Mon 3 mL (2 mL min.) Whole blood in 1 lavender top N116 DYSTROPHY MLPA 11 AM; Report (EDTA) tube. Ship refrigerated. DO NOT FREEZE. (DMD / BMD) Sat GENE MUTATION ECHINOCOCCUS Also see Sample by Tue / (HYDATID 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme ECHINOCOCCUS S036 Sat 9 am; SEROLOGY) refrigerated or frozen. Immunoassay DETECTION FOR ReportSame day. IgG, SERUM SCOLICES. ECHINOCOCCUS DETECTION Sample Daily by 6 1 mL (0.5 mL min.) Cyst fluid in a sterile screw P096 FOR SCOLICES Microscopy pm; Report Next capped container. Ship refrigerated or frozen. IN HYDATID day CYST FLUID ECHOVIRUS Sample by Mon 9 ANTIBODIES 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z218 am; Report Same PANEL, IgG & refrigerated or frozen. Immunoassay day IgM Sample by Mon 9 ECHOVIRUS 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S177 am; Report Same ANTIBODY, IgM refrigerated or frozen. Immunoassay day

PAGE 134 OF 399 Sample by Mon 9 ECHOVIRUS 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S125 am; Report Same ANTIBODY, IgG refrigerated or frozen. Immunoassay day Submit formalin fixed paraffin embedded block EGFR, Paraffin block must containing tumor tissue. Ship at room temperature. PCR, Fragment Sample by Tue 11 N072 MUTATIONAL contain minimum Provide a copy of the Histopathology report. Analysis, Sequencing am; Report Mon ANALYSIS 10% tumor cells Indicate site of biopsy and provide clinical history ELECTROLYTES, *Sodium 10 mL (5 mL min.) aliquot of random urine in a Indirect Ion Z380 RANDOM *Potassium sterile screw capped container. Ship refrigerated or Daily Selective Electrode URINE *Chloride frozen. ELECTRON Submit Kidney/ Muscle/ Nerve /Other tissue Sample Daily by 6 Transmission J154 MICROSCOPY biopsies in 3% Buffered Glutaraldehyde solution pm; Report 7 Electron microscopy (TEM) available from LPL. Ship refrigerated. days *Sodium 10 mL (5 mL min.) aliquot of 24-hour urine in a ELECTROLYTES, Indirect Ion Z383 *Potassium sterile screw capped container. Ship refrigerated or Daily 24 HOUR URINE Selective Electrode *Chloride frozen. *Sodium 3 mL (1.5 mL min.) serum from 1 SST. Avoid ELECTROLYTES, Indirect Ion Z020 *Potassium hemolysis. Ship refrigerated or frozen. Separate Daily SERUM Selective Electrode *Chloride serum within 1 hour of collection. ENDOMYSIAL For SERIAL Sample Daily by 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence S078 ANTIBODY, DILUTIONS use test 11 am; Report refrigerated or frozen. Overnight fasting is preferred. Assay IgA, IFA code S105. Same Day ENDOMYSIAL Sample Daily by ANTIBODY, 3 mL (1.5 mL min.) serum from 1 SST. Ship Immunofluorescence S105 11 am; Report IgA, IFA IN refrigerated or frozen. Overnight fasting is preferred. Assay Next day DILUTIONS

PAGE 135 OF 399 *Hyaluronic acid (HA) * Amino terminal Propeptide type ENHANCED 3 mL (1.5 mL min.) serum from 1 SST. Ship Chemiluminescent Sample by Mon / III Procollagen Z819 LIVER FIBROSIS refrigerated or frozen. Overnight fasting is Microparticle Thu 4 pm; Report (PIIINP) *Tissue (ELF) PANEL mandatory. Immunoassay Next day inhibitor Metalloproteinas e 1 (TIMP-1) * ELF score

ENTEROVIRUS 5 g fresh STOOL in a sterile screw capped container. Sample Daily by 1 M210 ANTIGEN, ICT Ship refrigerated only. DO NOT FREEZE. pmR Same day STOOL ENTEROHEMOR RHAGIC E.COLI, 5 g fresh STOOL in a sterile screw capped container. Sample Daily by 1 M204 ICT VEROTOXIN 1 Ship refrigerated only. DO NOT FREEZE. pmR Same day & 2 ANTIGEN ENTEROVIRUSE 3ml (2mL min.) whole blood from 1 Lavender Top Sample by S, N125 (EDTA) tube OR 3ml (2mL min.) CSF. Ship Real Time PCR Mon/Thu 11 am; QUALITATIVE, refrigerated. DO NOT FREEZE. Report Wed/Sat PCR EPIDERMAL For SERIAL Sample Daily by 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence S195 (PEMPHIGUS) DILUTIONS use test 11 am; Report refrigerated or frozen. Overnight fasting is preferred. Assay ANTIBODY, IFA code S198. Same day

EPIDERMAL Sample Daily by (PEMPHIGUS) 3 mL (1.5 mL min.) serum from 1 SST. Ship Immunofluorescence S198 11 am; Report ANTIBODY, IFA refrigerated or frozen. Overnight fasting is preferred. Assay Nextday IN DILUTIONS

PAGE 136 OF 399 EPSTEIN BARR 3 ml (2 mL min.) whole blood from 1 Lavender Top Sample by VIRUS (EBV), N127 (EDTA) tube OR 3 ml (2 mL min.) CSF. Ship Real Time PCR Mon/Thu 11 am; QUALITATIVE refrigerated. DO NOT FREEZE. Report Wed/Sat PCR EPSTEIN-BARR VIRUS, Sample by 7th of ANTIBODY TO 2 mL (1 mL min) serum from 1 SST. Ship refrigerated the month; RW067 Immunoassay EARLY or frozen. Reportafter 2–3 ANTIGEN (D), weeks IgG EPSTEIN-BARR VIRUS Sample by Wed / ANTIBODY TO 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S070 Sat 9 am; Report VIRAL CAPSID refrigerated or frozen. Immunoassay Same day ANTIGEN (VCA), IgM EPSTEIN BARR VIRUS ANTIBODIES TO Sample by Wed / 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z172 VIRAL CAPSID Sat 9 am; Report refrigerated or frozen. Immunoassay ANTIGEN Same day (VCA), IgG & IgM EPSTEIN-BARR VIRUS Sample by Wed / ANTIBODY TO 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S069 Sat 9 am; Report VIRAL CAPSID refrigerated or frozen. Immunoassay Same day ANTIGEN (VCA), IgG

PAGE 137 OF 399 EPSTEIN-BARR VIRUS, Sample by 7th of ANTIBODY TO 2 mL (0.5 mL min.) serum from 1 SST. Separate the month; RW068 Immunoassay NUCLEAR serum immediately. Ship refrigerated or frozen. Reportafter 2–3 ANTIGEN weeks (EBNA), IgG Sample by Mon / ERYTHROPOIETI 2 mL (0.5 mL min.) serum from 1 SST. Ship Chemiluminescent R018 Thu 4 pm; N; EPO refrigerated or frozen. Immunoassay ReportSame day ESR; 3 mL (1.5 mL min.) fasting whole blood in 1 Capillary ERYTHROCYTES H009 Lavender Top (EDTA) tube. Ship refrigerated. DO Photometry / Daily EDIMENTATION NOT FREEZE. Westergren RATE ESTRADIOL 3 mL (1 mL min.) serum from 1 SST. Specify age and Chemiluminescent R019 Daily (E2), SERUM sex on test request form. Ship refrigerated or frozen. Immunoassay

ESTRIOL Also see MATERNAL 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent R101 UNCONJUGATE SERUM SCREEN3 Daily refrigerated or frozen. Immunoassay D / FREE (µE3) and 4.

Submit tumour tissue in 10% Formal saline OR Sample Daily by 6 Includes Formalin fixed parafiin embedded block OR Alcohol pm; Report Block pathologist Use test code J113 ESTROGEN fixed Fine Needle Aspirate / Cytology Smears OR 5 Immunohistochemist / Section/ J009 review for the for RECEPTOR; ER unstained sections on Poly-L-Lysine Coated Slides. ry Smears : 5 days presence of Photomicrograph. Ship at room temperature. Provide a copy of the Tissues malignant cells. Histopathology / Cytopathology report. : 7 days Sample by 7th of ESTROGEN, 3 mL (1.5 mL min.) serum from 1 Red Top (No Extraction, the month; RW069 TOTAL, SERUM Additive) tube. Ship refrigerated or frozen. Radioimmunoassay Reportafter 2–3 weeks

PAGE 138 OF 399 2 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th of Additive) tube. Do not use SST gel barrier tubes. the month; RW070 ETHOSUXIMIDE Immunoassay Ship refrigerated or frozen. Ideal sampling time: Reportafter 2–3 Trough level, just prior to next dose. weeks

3 mL (2 mL min.) whole blood in 1 Lavender Top Sample Daily by 5 G161 EVEROLIMUS (EDTA) tube. Ship refrigerated. Ideal sampling time: LC-MS/MS pm. Report Same 1 hour before next dose (12 Hour Trough) Day

*Glucose (F ) *Creatinine *Uric Acid *Cholesterol *Triglycerides *SGOT *SGPT 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium *GGTP Fluoride) tube for Glucose, F AND 4 mL (2 mL min.) Spectrophotometry, EXECUTIVE *Bilirubin, Total serum from 1 SST AND 3 mL (2 mL min.) whole Electrical Z011 OFFICERS Daily *Protein, Total blood from 1 Lavender Top (EDTA) tube. Ship Impedence, VCS, PANEL *Albumin *A:G refrigerated. 12 hours overnight fasting is EMT Ratio *Alkaline mandatory. Phosphatase *CBC *Blood Group & Rh factor *TSH Ultrasensitive EXTRACTABLE Also see NUCLEAR Combined EXTRACTABLE Sample Mon ANTIGENS group screening 2 mL (1 mL min.) serum from 1 SST. Ship NUCLEARANTIGENS through Sat by 9 S118 Line Immunoassay (ENA), test for 15 refrigerated or frozen. (ENA), am; Report Same QUALITATIVE antigens QUANTITATIVEPROFI day PROFILE LE

PAGE 139 OF 399 EXTRACTABLE Individual NUCLEAR antibodies to Sample by Tue / ANTIGENS *Sm *U1RNP 3 mL (1.5 mL min.) serum from 1 SST. Ship Enzyme Z101 Fri 9 am; Report (ENA), *SS-A/Ro *SS- refrigerated or frozen. Immunoassay Same day QUANTITATIVE B/La *Jo-1 *Scl- PROFILE 70 *Centromere

10 mL (5–7 mL min.) whole blood from 3 Lavender FABRY DISEASE, This tests deficiency Sample Daily by 4 Top (EDTA) / Green Top (Sodium Heparin) tubes. G127 QUANTITATIVE, Enzyme Assay of the enzyme Alpha pm; Report 4 Ship refrigerated. DO NOT FREEZE.Clinical details BLOOD Galactosidase A. Days must accompany sample.

It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium Citrate) discontinues tube. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral 1 hour of collection as follows: Centrifuge sample at Anticoagulants for 7 3600 rpm for 15 min & transfer supernatant to a Sample by Thu 9 FACTOR II, Photo-optical Clot days prior to H173 clean plastic tube. Centrifuge this supernatant again am; Report Next FUNCTIONAL Detection sampling as these at 3600 rpm for 15 mins & finally transfer the day drugs may affect supernatant (PPP) to 1 labelled, clean plastic screw test results. capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician.

PAGE 140 OF 399 It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium Citrate) discontinues tube. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral 1 hour of collection as follows: Centrifuge sample at Anticoagulants for 7 3600 rpm for 15 min & transfer supernatant to a Sample by Thu 9 FACTOR V, Electromechanical days prior to H174 clean plastic tube. Centrifuge this supernatant again am; Report Next FUNCTIONAL Clot Detection sampling as these at 3600 rpm for 15 mins & finally transfer the day drugs may affect supernatant (PPP) to 1 labelled, clean plastic screw test results. capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician.

It is recommended that patient discontinues 3 mL Whole blood in 1 Blue Top(Sodium Citrate) Heparin for 1 day tube. Mix thoroughlyby inversion. Transport to Lab and Oral within 4 hrs. If this is not possible, make PPP within Anticoagulants for 7 1 hour of collection as follows: Centrifuge sample at days prior to 3600 rpm for 15 min & transfer supernatant to a Sample by Wed / FACTOR VIII Photo-Optical Clot sampling as these H240 clean plastic tube. Centrifuge this supernatant again Sat 9 am; Report FUNCTIONAL Detection drugs may affect at 3600 rpm for 15 mins & finally transfer the Fri/ Tue test results. supernatant (PPP) to 1 labelled, clean plastic screw Discontinuation capped vial. FREEZE IMMEDIATELY. Ship frozen. DO should be with prior NOT THAW. Overnight fasting is preferred. Duly consent from the filled Coagulation Requisition Form is mandatory. treating Physician. Also see COAGULATION PÇ

PAGE 141 OF 399 It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium Citrate) discontinues tube. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral 1 hour of collection as follows: Centrifuge sample at Anticoagulants for 7 3600 rpm for 15 min & transfer supernatant to a Sample by Thu 9 FACTOR X, Electromechanical days prior to H176 clean plastic tube. Centrifuge this supernatant again am; Report Next FUNCTIONAL Clot Detection sampling as these at 3600 rpm for 15 mins & finally transfer the day drugs may affect supernatant (PPP) to 1 labelled, clean plastic screw test results. capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician.

It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium Citrate) discontinues tube. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral 1 hour of collection as follows:Centrifuge sample at Anticoagulants for 7 3600 rpm for 15 min & transfer supernatant to a Sample by Thu 9 FACTOR XI, Photo-optical Clot days prior to H177 clean plastic tube. Centrifuge this supernatant again am; Report Next FUNCTIONAL Detection sampling as these at 3600 rpm for 15 mins & finally transfer the day drugs may affect supernatant (PPP) to 1 labelled, clean plastic screw test results. capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician

PAGE 142 OF 399 It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium Citrate) discontinues tube. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral 1 hour of collection as follows: Centrifuge sample at Anticoagulants for 7 3600 rpm for 15 min & transfer supernatant to a Sample by Thu 9 FACTOR XII, Photo-optical Clot days prior to H179 clean plastic tube. Centrifuge this supernatant again am; Report Next FUNCTIONAL Detection sampling as these at 3600 rpm for 15 mins & finally transfer the day drugs may affect supernatant (PPP) to 1labelled, clean plastic screw test results. capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician.

It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium Citrate) discontinues tube. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral FACTOR XIII 1 hour of collection as follows: Centrifuge sample at Anticoagulants for 7 (CLOT 3600 rpm for 15 min & transfer supernatant to a Sample Daily by 1 Clot Dissolution with days prior to H041 SOLUBILITY), clean plastic tube. Centrifuge this supernatant again pm; Report Next 5M Urea sampling as these FUNCTIONAL, at 3600 rpm for 15 mins & finally transfer the day drugs may affect QUALITATIVE supernatant (PPP) to 1 labelled, clean plastic screw test results. capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician.

PAGE 143 OF 399 It is recommended that patient discontinues 3 mL Whole blood in 1 Blue Top (Sodium Citrate) Heparin for 1 day tube. Mix thoroughly by inversion. Transport to Lab and Oral within 4 hrs. If this is not possible, make PPP within Anticoagulants for 7 1 hour of collection as follows: Centrifuge sample at days prior to 3600 rpm for 15 min & transfer supernatant to a Sample by Wed / FACTOR IX, Photo-optical Clot sampling as these H175 clean plastic tube. Centrifuge this supernatant again Sat 9 am; Report FUNCTIONAL Detection drugs may affect at 3600 rpm for 15 mins & finally transfer the Fri/ Tue test results. supernatant (PPP) to 1 labelled, clean plastic screw Discontinuation capped vial. FREEZE IMMEDIATELY. Ship frozen. DO should be with prior NOT THAW. Overnight fasting is preferred. Duly consent from the filled Coagulation Requisition Form is mandatory. treating Physician. Also see HEMOPHILIA PANEL.

*Factor V Leiden FACTOR V Mutation Sample by Mon / LEIDEN *Prothrombin 3 mL (2 mL min.) Whole blood in 1Lavender Top H209 Real Time PCR Thu 11 am; MUTATION Gene Mutation (EDTA) tube. Shiprefrigerated. DO NOT FREEZE. ReportWed / Sat ANALYSIS *MTHFR Gene Mutation

PAGE 144 OF 399 It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium Citrate) discontinues tube. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral 1 hour of collection as follows: Centrifuge sample at Anticoagulants for 7 3600 rpm for 15 min & transfer supernatant to a Sample by Thu 9 FACTOR VII Photo-optical Clot days prior to H178 clean plastic tube. Centrifuge this supernatant again am; Report Next FUNCTIONAL Detection sampling as these at 3600 rpm for 15 mins & finally transfer the day drugs may affect supernatant (PPP) to 1 labelled, clean plastic screw test results. capped vial. FREEZE IMMEDIATELY. Ship frozen.DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician.

It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium Citrate) discontinues tube. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral 1 hour of collection as follows: Centrifuge sample at Anticoagulants for 7 3600 rpm for 15 min & transfer supernatant to a Sample by Wed / FACTOR VIII Electromechanical days prior to H247 clean plastic tube. Centrifuge this supernatant again Sat 9 am; Report INHIBITOR Clot Detection, EIA sampling as these at 3600 rpm for 15 mins & finally transfer the Fri/ Tue drugs may affect supernatant (PPP) to 1 labelled, clean plastic screw test results. capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician.

PAGE 145 OF 399 FANCONI'S 5 mL (3 mL min.) whole blood from 1 Green Top Sample Daily by 6 ANEMIA, XX025 (Sodium Heparin) tube. Ship at 18-22°C. DO NOT Stress Cytogenetics pm; Report 7 STRESS FREEZE Days CYTOGENETICS 2 mL (1 mL min.) serum from 1 SST. Ship Sample by Mon FATTY LIVER *GGTP refrigerated or frozen. Overnight fasting is through Sat 9 Z837 IFCC,GPO-POD INDEX *Triglycerides mandatory. Record patient height, weight, waist am; Report same circumference on test request form. day *Brucella abortus *Brucella FEBRILE melitensis AGGLUTININS *Salmonella Sample Daily by 6 PANELCombine 3 mL (2 mL min.) serum from 1 SST. Ship Latex & Tube Also see BRUCELLA Z124 paratyphi Type pm; Report Next d Brucella, refrigerated or frozen. Agglutination ANTIBODY, IgG& IgM AH and Type BH day Widal and Weil *Salmonella Felix tests panel typhi H & O *OX2 *OXK *OX19 Sample by Mon 9 Submit 5g (2g min.) Stool in a clean dry container. Enzyme S247 FECAL ELASTASE am; Report Same Ship refrigerated or frozen. Immunoassay day. Sample by Mon 9 FECAL Submit 5g (2g min.) Stool in a clean dry container. Enzyme S231 am; Report Same CALPROTECTIN Ship refrigerated. DO NOT FREEZE. Immunoassay day 2 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th of Additive) tube. Ship refrigerated or frozen. Ideal the month; RW071 FELBAMATE LC-MS/MS sampling time: 1 hour before next dose. Do not use Reportafter 2–3 SST gel barrier tubes. weeks

PAGE 146 OF 399 Also see AMINO FERRIC Sample Daily by 5 10 mL (5 mL min.) aliquot of random urine. Ship ACID PANELS, G141 CHLORIDE Chemical pm; Report Same refrigerated or frozen. QUALITATIVE AND TEST, URINE day QUANTITATIVE. 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent H060 FERRITIN Daily refrigerated or frozen. Immunoassay 3 mL (2 mL min.) whole blood in 1 Lavender Top Electrical *Hemogram (EDTA) tube AND 2 mL (1 mL min.) serum from 1 impedence, VCS, Z390 FEVER PANEL 1 *MP *Widal Daily SST AND 10 mL (5 mL min.) aliquot of random urine. Microscopy,Agglutin *Urine R/E Ship refrigerated. Overnight fasting is preferred ation, Dipstick Electrical 3 mL (2 mL min.) whole blood in 1 Lavender Top impedence, VCS, *Hemogram (EDTA) tube AND 2 mL (1 mL min.) serum from 1 Microscopy, Sample Daily by 6 *MP *Widal SST AND 10 mL (5 mL min.) aliquot of random urine. Agglutination, pm;Report Z391 FEVER PANEL 2 *Urine R/E Ship refrigerated AND 10 mL Blood collected Dipstick, IFCC, Interim : 24 hrs & *SGPT * Aerobic aseptically in Special Plus Aerobic bottle available Conventional 48 hrs Blood Culture from LPL. Ship at 18–22°C. Overnight fasting is culture & Final : 6 Days preferred. Automated sensitivity

3 mL Whole blood in 1 Blue Top (Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hrs. If this is not possible, make PPP within 1 hour of collection as follows: Centrifuge sample at FIBRINOGEN, 3600 rpm for 15 min & transfer supernatant to a Photo-optical Clot B088 CLOTTING clean plastic tube. Centrifuge this supernatant again Daily Detection ACTIVITY at 3600 rpm for 15 mins & finally transfer the supernatant (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form is mandatory.

PAGE 147 OF 399 3 mL Whole blood in 1 Blue Top (Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hrs. If this is not possible, make PPP within 1 hour of collection as follows: Centrifuge sample at FIBRINOGEN 3600 rpm for 15 min & transfer supernatant to a Also see D-DIMER & DEGRADATION H026 clean plastic tube. Centrifuge this supernatant again Latex Agglutination DEEP VEIN Daily PRODUCTS at 3600 rpm for 15 mins & finally transfer the THROMBOSIS PANEL (FDP), BLOOD supernatant (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen.DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form is mandatory.

2 mL (1 mL min.) Plasma from 1 Lavender Top Sample by 7th of FIBROBLAST (EDTA) tube. Separate plasma within 6 hours of the month; RW206 GROWTH Immunoassay collection in a cold centrifuge. Freeze immediately. Reportafter 2–3 FACTOR; FGF2 Ship frozen. DO NOT THAW. weeks

*CMV, IgG *Selenium, If Gadolinium or Serum * RA Iodine containing factor * Anti contrast media has Nuclear 4 mL ( 3 mL min.) serum from 1 SST AND 2 mL (1 mL ICPMS, CLIA, been administered, Sample Daily by 6 FIBROMYALGIA Z800 Antibody (ANA) min.) serum from 1 White top (Z-No Additive) tube Immunoturbidimetry specimen should not pm; Report Next PANEL * Creatine available from LPL. Ship refrigerated or frozen. , IFCC, EIA be collected for 96 day Phosphokinase hours Use only (CPK), TSH, specified containers Ultrasensitive *C- available from LPL. Reactive Protein

2 mL (1 mL min.) whole blood from 1 Green Top FILARIA Immunochromatogr S170 (Sodium Heparin) tube OR 2 mL (1 mL min.) serum Daily ANTIGEN aphy from 1 SST. Ship refrigerated. DO NOT FREEZE.

PAGE 148 OF 399 FIP1L1-PGDFRA 4 ml (2 ml min.) Whole blood / Bone marrow from Sample by Tue 11 GENE OS135 1 Lavender Top (EDTA) tube. Ship refrigerated. DO PCR am; Report 10 REARRANGEME NOT FREEZE days NT DETECTION

FISH - ALK-1 Sample Daily by 4 Formalin fixed paraffin embedded tissue block. Ship XX031 Breakapart FISH pm; Report 7 at room temperature Rearrangement Days. FISH - Amnio 5 mL Amniotic fluid in a sterile screw capped Consent form for one probe Sample Daily by 4 container. Ship refrigerated. DO NOT FREEZE. Prenatal Genetic XX015 (Trisomy 21) / FISH pm; Report 7 Clinical history mandatory. Consent form for Testing is Down Days. Prenatal Genetic Testing is mandatory. mandatory. syndrome FISH - Amnio 5 mL Amniotic fluid in a sterile screw capped Consent form for Sample Daily by 4 three probes container. Ship refrigerated. DO NOT FREEZE. Prenatal Genetic XX017 FISH pm; Report 7 (Trisomy 18, X, Clinical history mandatory. Consent form for Testing is Days. Y) Prenatal Genetic Testing is mandatory. mandatory. 5 mL Amniotic fluid in a sterile screw capped Consent form for FISH - Amnio Sample Daily by 4 container. Ship refrigerated. DO NOT FREEZE. Prenatal Genetic XX016 two probes FISH pm; Report 7 Clinical history mandatory. Consent form for Testing is (Trisomy 13; 21) Days. Prenatal Genetic Testing is mandatory. mandatory. FISH - Aneuploidy Submit Placenta (villi) or Fascia lata, diaphragm, detection, tendon, skin, tissue from internal organs (if fresh), Products of chest wall cartilage (particularly if macerated) in Sample Daily by 4 XX036 Conception normal saline.Ship immediately at 18-22°C. FISH pm; Report 7 (POC) using Formalin fixed specimen not acceptable. Give Days. chromosomes clinical history on Chromosome Analysis Test 13,18, 21, X Request Form. and Y

PAGE 149 OF 399 FISH - BCR / 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) Sample Daily by 4 ABL or Bone Marrow from 2 Green Top (Sodium Heparin) XX001 FISH pm; Report 7 Philadelphia tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical Days. translocation history mandatory. FISH - Bladder cancer recurrence: Submit 10 mL Urine in a sterile screw capped Sample Daily by 4 XX024 Chromosomes container. Ship refrigerated. DO NOT FREEZE. FISH pm; Report 7 3,7, 17 & p16 Clinical history mandatory. Days. gene on chromosome 9

*del11q23(ATM) *del17p13.1(TP5 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) 3) *del13q14.3 Sample Daily by 4 Bone Marrow from 2 Green Top (Sodium Heparin) XX023 FISH - CLL Panel *del13q34 FISH pm; Report 7 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical *trisomy 12 Days. history mandatory. *IGH Gene breakapart

5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) Sample Daily by 4 Bone Marrow from 2 Green Top (Sodium Heparin) XX029 FISH - C-MYC FISH pm; Report 7 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical Days. history mandatory. 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) Sample Daily by 4 FISH - del 13q Bone Marrow from 2 Green Top (Sodium Heparin) XX005 FISH pm; Report 7 or LSI D 13S319 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical Days. history mandatory. FISH - EGFR Sample Daily by 4 Formalin fixed paraffin embedded tissue block. Ship XX012 Gene FISH pm; Report 7 at room temperature amplification Days.

PAGE 150 OF 399 Formalin fixed paraffin embedded tissue block / FISH- Sample Daily by 4 three 4µ sections on Poly-L-Lysine coated slides. XX051 EQUIVOCAL FISH pm; Report 5 Ship at room temperature. Clinical history HER2 (ERBB2) days mandatory. FISH-EWING SARCOMA Formalin fixed paraffin embedded tissue block / Sample Daily by 4 (EWS), 22q12 three 4µ sections on Poly-L-Lysine coated slides. XX048 FISH pm; Report 7 (EWSR1) Ship at room temperature. Clinical history days REARRANGEME mandatory. NT

FISH- Eosinophilic 5 mL (3 mL min.) Bone marrow OR 5 mL ( 3 mL Sample Daily by 4 Leukemia Panel min.) Whole blood in 1 Green Top (Sodium Heparin) XX045 FISH pm; Report 7 (PDGFRA, tube. Ship at 18-22°C. DO NOT FREEZE. Clinical days PDGFRB, FGFR1 history mandatory. and CBFB)

FISH-FGFR1 5 mL (3 mL min.) Bone marrow OR 5 mL ( 3 mL Sample Daily by 4 (8p12) GENE min.) Whole blood in 1 Green Top (Sodium Heparin) XX044 FISH pm; Report 7 REARRANGEME tube. Ship at 18-22°C. DO NOT FREEZE. Clinical days NT history mandatory. FISH - HER2 Sample Daily by 4 Formalin fixed paraffin embedded tissue block. Ship XX011 (ERBB2) FISH pm; Report 7 at room temperature amplification Days. 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) Sample Daily by 4 FISH - inv (16) Bone Marrow from 2 Green Top (Sodium Heparin) XX004 FISH pm; Report 7 or LSI CBFB tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical Days. history mandatory. FISH - MDS 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) Panel - Sample Daily by 4 Bone Marrow from 2 Green Top (Sodium Heparin) XX022 Chromosomes FISH pm; Report 7 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical 5q, 7q, 8q & Days. history mandatory. 20q.

PAGE 151 OF 399 Formalin fixed paraffin embedded tissue block / FISH-MET Sample Daily by 4 three 4µ sections on Poly-L-Lysine coated slides. XX050 (7q31) FISH pm; Report 7 Ship at room temperature. Clinical history AMPLIFICATION Days. mandatory. Del 13q14.3,del 17p13.1, t(11;14) 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) FISH - Multiple Sample Daily by 4 CCND1/IGH, Bone Marrow from 2 Green Top (Sodium Heparin) XX027 Myeloma (5 FISH pm; Report 7 t(4;14) tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical Probes) Days. FGFR3/IGH, and history mandatory. t(14;16) IGH/MAF Submit formalin fixed paraffin embedded tissue Sample Daily by 4 FISH-MYCN XX046 block / three 4 µ sections on Poly-L-Lysine coated FISH pm; Report 7 AMPLIFICATION slides. Clinical history mandatory. days FISH - Microdeletion 5 mL (3 mL min.) whole blood from 1 Green Top Sample Daily by 4 XX038 detection for (Sodium Heparin) tube. Ship at 18-22°C. DO NOT FISH pm; Report 7 Williams FREEZE. Clinical history mandatory. Days. syndrome Del 13q14.3,del 17p13.1, FISH - Multiple t(11;14) 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) Myeloma with Sample Daily by 4 CCND1/IGH, Bone Marrow from 2 Green Top (Sodium Heparin) XX032 CD138 positive FISH pm; Report 7 t(4;14) tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical selection cells Days. FGFR3/IGH, and history mandatory. ( 5 Probes) t(14;16) IGH/MAF FISH - NSCLC Formalin fixed paraffin embedded tissue block / Sample Daily by 4 (Non-small cell Z787 three 4u sections on Poly-L-Lysine coated slides. FISH pm; Report 10 lung cancer) Ship at room temperature. Days ALK1 & ROS1

PAGE 152 OF 399 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) FISH - Opposite Sample Daily by 4 Bone Marrow from 2 Green Top (Sodium Heparin) XX010 Sex BMT (XX / FISH pm; Report 7 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical XY) Days. history mandatory. FISH - Prader 5 mL (3 mL min.) whole blood from 1 Green Top Sample Daily by 4 XX018 Willi Syndrome (Sodium Heparin) tube. Ship at 18-22°C. DO NOT FISH pm; Report 7 SNRPN FREEZE. Clinical history mandatory. Days.

FISH - Prenatal 6 mL Amniotic fluid in a sterile screw capped Consent form for Sample Daily by 4 screening panel container. Ship refrigerated. DO NOT FREEZE. Prenatal Genetic XX030 FISH pm; Report 7 (13,18,21,X Clinical history mandatory. Consent form for Testing is Days. and Y) Prenatal Genetic Testing is mandatory. mandatory.

FISH-PDGFR 4 mL (3 mL min.) Bone marrow OR 4 mL ( 3 mL min.) alpha (4q12) Sample Daily by 4 Whole blood in 1 Green Top (Sodium Heparin) tube. XX042 GENE FISH pm; Report 7 Ship at 18-22°C. DO NOT FREEZE. Clinical history REARRANGEME days mandatory. NT FISH-PDGFR 4 mL (3 mL min.) Bone marrow OR 4 mL ( 3 mL min.) beta (5q33) Sample Daily by 4 Whole blood in 1 Green Top (Sodium Heparin) tube. XX043 GENE FISH pm; Report 7 Ship at 18-22°C. DO NOT FREEZE. Clinical history REARRANGEME days mandatory. NT Sample Daily by 4 FISH - 1p and Formalin fixed paraffin embedded tissue block. Ship XX013 FISH pm; Report 7 19q Codeletion at room temperature Days. 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) Sample Daily by 4 Bone Marrow from 2 Green Top (Sodium Heparin) XX026 FISH - 17 p FISH pm; Report 7 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical Days. history mandatory.

PAGE 153 OF 399 FISH - 22q 5 mL (3 mL min.) whole blood from 1 Green Top Sample Daily by 4 deletion or LSI XX014 (Sodium Heparin) tube. Ship at 18-22°C. DO NOT FISH pm; Report 7 Di George / FREEZE. Clinical history mandatory. Days. VCFS 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) FISH - 11q 23 or Sample Daily by 4 Bone Marrow from 2 Green Top (Sodium Heparin) XX007 LSI MLL Gene FISH pm; Report 7 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical breakapart Days. history mandatory. 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) FISH - 14q 32.3 Sample Daily by 4 Bone Marrow from 2 Green Top (Sodium Heparin) XX009 or LSI IGH Gene FISH pm; Report 7 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical breakapart Days. history mandatory. FISH- ROS1 Formalin fixed paraffin embedded tissue block / Sample Daily by 4 (6Q22) three 4µ sections on Poly-L-Lysine coated slides. XX049 FISH pm; Report 7 REARRANGEME Ship at room temperature. Clinical history days NT mandatory. FISH - SYNOVIAL SARCOMA Submit formalin fixed paraffin embedded tissue Sample Daily by 4 XX047 (SS18 / SYT ) block / three 4 µ sections on Poly-L-Lysine coated FISH pm; Report 7 18q11.2 GENE slides. Clinical history mandatory. days REARRANGEME NT 5 mL (3 mL min.) whole blood from 1 Green Top Sample Daily by 4 XX028 FISH - SRYGENE (Sodium Heparin) tube. Ship at 18-22°C. DO NOT FISH pm; Report 7 FREEZE. Clinical history mandatory. Days. 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) FISH - t(8;21) Sample Daily by 4 Bone Marrow from 2 Green Top (Sodium Heparin) XX003 or LSI FISH pm; Report 7 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical ETO/AML1 Days. history mandatory.

PAGE 154 OF 399 FISH - t(8;14) or 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) MYC/IGH for Sample Daily by 4 Bone Marrow from 2 Green Top (Sodium Heparin) XX039 Burkitt's & Non- FISH pm; Report 7 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical Hodgkin Days. history mandatory. lymphoma 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) FISH - t(11;14) Sample Daily by 4 Bone Marrow from 2 Green Top (Sodium Heparin) XX006 or LSI FISH pm; Report 7 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical IGH/CCNDI Days. history mandatory. 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) Sample Daily by 4 FISH - t (12;21) Bone Marrow from 2 Green Top (Sodium Heparin) XX008 FISH pm; Report 7 or LSI TEL/AML1 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical Days. history mandatory. 5 mL (3 mL min.) whole blood OR 4 mL ( 2 mL min.) FISH - t (15;17) Sample Daily by 4 Bone Marrow from 2 Green Top (Sodium Heparin) XX002 or LSI FISH pm; Report 7 tubes. Ship at 18-22°C. DO NOT FREEZE. Clinical PML/RARA Days. history mandatory. 5 mL (3 mL min.) Whole blood / Cord blood from 2 Green Top (Sodium Heparin) tubes OR 5 mL (3 mL Consent form for FISH - Trisomy Sample Daily by 4 min.) Amniotic fluid in a sterile screw capped Prenatal Genetic XX037 21 / Down FISH pm; Report 7 container OR 2g Chorionic villus biopsy in normal Testing is syndrome Days. saline. Ship at specified temperatures.Consent form mandatory. for Prenatal Genetic Testing is mandatory. 3 mL (1.5 mL min) serum from 1 Red Top (No Sample by 7th of Additive) tube. Do not use SST gel barrier tubes. the month; RW072 FLECAINIDE LC-MS/MS Ship refrigerated or frozen. Ideal sampling time: 1 Reportafter 2–3 hour before next dose. weeks

PAGE 155 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H151 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 25 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H232 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 123 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H149 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 22 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H222 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 79a at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form.

PAGE 156 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H142 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 11c at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H143 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 13 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H229 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 138 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H144 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 14 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form.

PAGE 157 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H145 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 15 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H156 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 41a at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H231 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 64 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H139 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 7 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form.

PAGE 158 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H223 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 117 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H138 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 5 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H162 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same HLA DR at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL CYTOMETRY, min.) Bone marrow in 1 Green Top (Sodium Sample Daily by 9 H237 CD 34 (STEM heparin) tube OR 1 mL(0.5mL min) Cord blood / Flow Cytometry am. Report Same CELL) Apheresis sample in a sterile screw capped day ENUMERATION container. Ship immediately at 2 –8°C. DO NOT FREEZE. Specify time, date and clinical details on test request form.

PAGE 159 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top FLOW (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 CYTOMETRY, Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H167 IgG Heavy min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same Chain at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day (Cytoplasmic) Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H141 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 10 (CALLA) at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H181 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 103 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 Z166A CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 16 + 56 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form.

PAGE 160 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H147 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 19 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 Z219A CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 4 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H236 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 79b at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H203 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD MPO at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form.

PAGE 161 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H160 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same FMC-7 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H136 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 3 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H154 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 34 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H230 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 36 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form.

PAGE 162 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H135 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 2 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H157 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 45 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H161 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same GLYCOPHORIN at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H153 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 33 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form.

PAGE 163 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H155 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 38 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 Z147A CYTOMETRY, - min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 8 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H172 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same TdT at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H228 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same ZAP-70 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form.

PAGE 164 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H158 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 61 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 CYTOMETRY, H168 min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same IgG Heavy at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Chain (Surface) Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top FLOW (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 CYTOMETRY, Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H169 IgM Heavy min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same Chain at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day (Cytoplasmic) Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 CYTOMETRY, H170 min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same IgM Heavy at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Chain (Surface) Specify time, date and clinical details on test request form.

PAGE 165 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top FLOW (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 CYTOMETRY, Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H171 Kappa & min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same Lambda Light at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Chains Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H148 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 20 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H234 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 1a at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 FLOW Green Top (Sodium Heparin) tube OR 2 mL (1 mL Sample Daily by 9 H150 CYTOMETRY, min.) heparinized Bone marrow. Ship immediately Flow Cytometry am. Report Same CD 23 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. 5 mL (3 mL min.) Whole blood / Bone marrow in 1 Sample by Mon / FLT3 GENE Real Time PCR, N052 Lavender Top (EDTA) tube. Ship refrigerated. DO Thu 11 am ; MUTATION Fragment Analysis NOT FREEZE. Clinical history is mandatory. Report Wed / Sat

PAGE 166 OF 399 FLUID Biochemistry and 2 mL (1mL min.) Cerebrospinal fluid / Nasal fluid in a EXAMINATION, microscopy of P012 sterile screw capped container. Ship refrigerated. Daily ROUTINE, CSF/ Giemsa stained DO NOT FREEZE. NASAL FLUID smears FLUID 5 ml (2 mL min.) fluid in a sterile screw capped Biochemistry and EXAMINATION, container. Ship refrigerated. DO NOT FREEZE. microscopy of P013 Daily ROUTINE, Specify type of fluid on Test Request Form and Giemsa stained BODY FLUIDS container. smears 3 mL (1.5 mL min.) serum from 1 Red Top (No Sample by 7th of Includes Additive) tube. Do not use SST gel barrier tubes. the month; RW073 FLUOXETINE metabolite LC-MS/MS Ship refrigerated or frozen. Ideal sampling time: >12 Reportafter 2–3 Norfluoxetine. hours post oral dose. weeks 2ml(1 ml min.) serum form 1SST. Ship refrigerated or frozen. Provide maternal Date of Birth The test is done (dd/mm/yy), LMP, USG report between 11-13 using FMF approved FMF FIRST weeks gestation including CRL, NT & Nasal Bone, Sample by Tues / kits. Enclose Z781 TRIMESTER number of fetuses; Diabetic status, body weight; ECLIA Thu / Sat 9 am; demographic details SCREEN IVF, Smoking & Previous h/o Trisomy 21. Enclose Report same day in maternal serum Patient demographic details in Maternal Serum screen request form Screen Request Form. Valid between 9-13 weeks gestation (Ideal 10-13 weeks). 2 mL (1 mL min.) whole blood from 1 Lavender Top (EDTA) tube AND 2 mL (1 mL min.) serum from 1 Sample Daily by 2 FOLATE (FOLIC Includes Serum Chemiluminescent R048 Red Top (No Additive) tube. Wrap tubes in pm; Report Same ACID), RBC Folate. Immunoassay aluminium foil to protect from light. Ship Day refrigerated or frozen. 2 mL (1 mL min.) serum from 1 Red Top (No FOLATE (FOLIC Chemiluminescent R046 Additive) tube. Wrap tube in aluminium foil to Daily ACID), SERUM Immunoassay protect from light. Ship refrigerated or frozen.

PAGE 167 OF 399 FOOD Sample Daily by 1 2 mL (1 mL min.) serum from 1 SST. Ship A110 INTOLERANCE Protein Microarray pm; Report 2 refrigerated or frozen. TEST days *Creatinine, FRACTIONAL serum * 3 mL (2 mLmin.) serum from 1 SST AND 10 mL(5 mL Jaffe's reaction, B191 EXCRETION OF Creatinine, urine min) aliquot of random urine in a sterile screw Daily Indirect ISE SODIUM *Sodium, serum capped container. Ship refrigerated or frozen. *Sodium, urine FRAGILE X Sample by Tue 11 (FMR1) 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS137 PCR am; Report 10 MUTATION (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. days SCREEN 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent Also see MATERNAL B151 FREE BETA HCG Daily refrigerated or frozen. Immunoassay SERUM SCREEN 2. FREIDREICHS Sample by Tue 11 ATAXIA 4 mL (2 mL min.) Whole Blood in 1 Lavender top PCR Fragment OS138 am; Report 10 MUTATION (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. analysis days ANALYSIS Also see GLYCOSYLATED/GLY 2 mL (1 mL min.) serum from 1 SST. Ship CATEDHEMOGLOBIN B071 FRUCTOSAMINE Spectrophotometry Daily refrigerated or frozen. , LIPID PROFILE & MICROALBUMIN URINE. In females, FSH; FOLLICLE 2 mL (1 mL min.) serum from 1 SST. Ship preferred sampling Chemiluminesent R080 STIMULATING refrigerated or frozen. Specify age, sex and day of time is Day 2 / Day 3 Daily Immunoassay HORMONE menstrual cycle on test request form. of the menstrual cycle.

PAGE 168 OF 399 2 mL (1 mL min.) serum from 1 SST for each timed FSH & LH refer to Y specimen . Draw fasting baseline specimen; STIMULATION Codes:Y050 administer GnRH 2.5 ug/kg body weight (100 ug BY BasalY051 30 maximum) as IV bolus; draw additional specimens Chemiluminescent Z079 GONADOTROPI min.Y052 45 Daily at 30, 45 & 60 min for FSH and LH. Ship refrigerated Immunoassay NRELEASING min.Y053 60 or frozen. All containers must be clearly marked HORMONE min.Y054 with time drawn and submitted with one test (GnRH) Customized request form.

In females, 3 mL (1.5 mL min.) serum from 1 SST. Ship preferred sampling Chemiluminescent Z126 FSH & LH refrigerated or frozen. Specify age, sex & day of time is Day 2 / Day 3 Daily Immunoassay menstrual cycle on test request form. of the menstrual cycle.

Submit Skin, Nail scrapings, Hair ( including root), FUNGUS Cerebrospinal fluid, in a sterile screw capped EXAMINATION, container OR 2 mL whole blood / bone marrow in 1 Sample Daily by ROUTINE, KOH Green Top (Sodium Heparin) tube. Ship at 18–22°C. Fluorescent P021 3.30 pm; Report CALCOFLUOR Submit Body fluid, Sputum, Bronchial washings / microscopy Same day WHITE Tissue in a sterile screw capped container OR PREPARATION Oral/Genital/wound swabs in 0.5 mL sterile normal saline. Ship refrigerated. 2 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th of Additive) tube. Separate serum immediately. Ship the month; RW074 GABAPENTIN LC-MS/MS refrigerated or frozen. Ideal sampling time: 2 hours Reportafter 2–3 after last dose. weeks GAD-65 (GLUTAMIC Sample by Sat 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme B150 ACID am: Report Same refrigerated or frozen. Immunoassay DECARBOXYLAS day E- 65), IgG

PAGE 169 OF 399 4 mL (2 mL min.) whole blood from 1 Green Top *Galactosemia (Sodium Heparin) tube AND 4 mL (2 mL min.) Screening CONTROL blood Sample Daily by 4 GALACTOSEMIA CONTROL blood from 1 Green Top (Sodium Heparin) Spot Test, Enzyme G119 *Galactosemia should not be taken pm; Report 5 PANEL 1 tube. Ship refrigerated. DO NOT FREEZE. Avoid assay Classical from blood relatives. Days sample collection for 60 days post transfusion. (Transferase) Clinical and drug history must accompany sample.

4 mL (2 mL min.) whole blood from 1 Green Top *Galactosemia (Sodium Heparin) tube AND 4 mL (2 mL min.) Screening CONTROL blood from 1 Green Top (Sodium Heparin) *Galactosemia CONTROL blood Sample Daily by 4 GALACTOSEMIA tube AND 2 mL (1 mL min.) plasma from 1 Green Spot Test, Enzyme G120 Classical should not be taken pm; Report 5 PANEL 2 Top (Sodium Heparin) tube. Ship refrigerated. DO assay (Transferase) from blood relatives. days NOT FREEZE. Avoid sample collection for 60 days *Galactose post transfusion. Clinical and drug history must Plasma accompany sample. *Galactosemia 4 mL (2 mL min.) whole blood from 1 Green Top Classical (Sodium Heparin) tube. Ship refrigerated. DO NOT Sample Daily by 4 GALACTOSEMIA Spot Test, Enzyme G121 (Transferase)*Gal FREEZE. Avoid sample collection for 60 days post pm; Report 5 PANEL 3 assay actosemia transfusion. Clinical and drug history must days (Epimerase) accompany sample. GALACTOMANN Sample Mon AN 2 mL (1 mL min.) serum from 1 SST. Ship through Sat by S242 (ASPERGILLUS EIA refrigerated or frozen. 12 Noon; Report ANTIGEN), Same day SERUM 1 drop of heel prick blood each on 3 spots of filter GALACTOSEMIA Sample by Tue / paper available from LPL. Clinical details and drug R164 , NEWBORN Fluoroimmunoassay Thu / Sat 5 pm; history must accompany sample. Ship refrigerated SCREEN Report 2 days or frozen.

PAGE 170 OF 399 4 mL (2 mL min.) whole blood from 1 Green Top GALACTOSEMIA (Sodium Heparin) tube. Ship refrigerated. DO NOT Sample Daily by 4 (EPIMERASE), G118 FREEZE. Avoid sample collection for 60 days post Enzyme assay pm; Report 5 QUANTITATIVE, transfusion. Clinical and drug history must Days BLOOD accompany sample. GALACTOSEMIA Sample by Tue (GALT) GENE 4 mL (2 mL min.) Whole Blood from 1 Lavender top OS140 PCR Sequencing 11am; Report 11 MUTATION (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. days DETECTION GALACTOSEMIA 4 mL (2 mL min.) whole blood from 1 Green Top (Sodium Heparin) tube. Ship refrigerated. DO NOT Sample Daily by 4 CLASSICAL(TRA G117 FREEZE. Avoid sample collection for 60 days post Enzyme assay pm; Report 5 NSFERASE), transfusion. Clinical and drug history must Days QUANTITATIVE, accompany sample. BLOOD Sample must be 4 mL (2 mL min.) whole blood from 1 Green Top kept cold as GALACTOSE -1- (Sodium Heparin) tube. Immerse specimen Galactose -1- Sample Daily by 4 PHOSPHATE,QU G115 immediately in ice water. Ship refrigerated. DO NOT Fluorometry phosphate levels fall pm; Report 5 ANTITATIVE,BL FREEZE. Clinical and drug history must accompany upto 50% within 4 Days OOD sample. hours if kept at room temperature.

GALACTOSE, 2 mL (1 mL min.) plasma from 1 Green Top (Sodium Sample Daily by 4 G116 QUANTITATIVE, Heparin) tube. Ship refrigerated or frozen. Clinical / Fluorometry pm; Report 3 PLASMA drug history & diet must accompany sample. days

PAGE 171 OF 399 4 mL (2 mL min.) whole blood from 1 Green Top (Sodium Heparin) tube AND 4 mL (2 mL min.) GALACTOSEMIA Beutler CONTROL blood Sample Daily by 4 CONTROL blood from 1 Green Top (Sodium Heparin) G114 SCREENING, Fluorescence Spot should not be taken pm; Report Next tube. Ship refrigerated. DO NOT FREEZE. Avoid BLOOD Test from blood relatives. day sample collection for 60 days post transfusion. Clinical and drug history must accompany sample. GANCICLOVIR Sample by Tue 11 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS141 RESISTANCE PCR Sequencing am; Report 9 (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. DETECTION days Sample by 7th of GANGLIOSIDE 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme the month; RW075 GD1a refrigerated or frozen. Overnight fasting preferred. Immunoassay Reportafter 2–3 ANTIBODY, IgG weeks Sample by 7th of GANGLIOSIDE 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme the month; RW076 GD1a refrigerated or frozen. Overnight fasting preferred. Immunoassay Reportafter 2–3 ANTIBODY, IgM weeks Sample by 7th of GANGLIOSIDE 2 mL (1 mL min.) serum from 1 SST. Ship the month; RW080 GQ1B Immunoassay refrigerated or frozen. Overnight fasting is preferred. Reportafter 2–3 ANTIBODY, IgG weeks *GM1 *GM2 GANGLIOSIDE Sample by Tue 9 *GM3 *GD1a 2 mL (1 mL min.) serum from 1 SST. Ship S234 ANTIBODY Immunoblot am; Report Same *GD1b *GT1b refrigerated or frozen. PROFILE, IgM day *GQ1b Sample by 7th of GANGLIOSIDE 2 mL (1 mL min.) serum from 1 SST. Ship the month; RW077 GD1b Immunoassay refrigerated or frozen. Overnight fasting preferred. Reportafter 2–3 ANTIBODY, IgG weeks Sample by 7th of GANGLIOSIDE 2 mL (1 mL min.) serum from 1 SST. Ship the month; RW078 GD1b Immunoassay refrigerated or frozen. Overnight fasting preferred. Reportafter 2–3 ANTIBODY, IgM weeks

PAGE 172 OF 399 Sample by 7th of GANGLIOSIDE 2 mL (1 mL min.) serum from 1 SST. Ship the month; RW079 ANTIBODIES, Immunoassay refrigerated or frozen. Overnight fasting preferred. Reportafter 2–3 IgG & IgM weeks. *GM1 *GM2 GANGLIOSIDE Sample by Tue 9 *GM3 *GD1a 2 mL (1 mL min.) serum from 1 SST. Ship S233 ANTIBODY Immunoblot am; Report Same *GD1b *GT1b refrigerated or frozen. PROFILE, IgG day *GQ1b 2 mL (1 mL min.) serum from 1 SST. Ship frozen. DO NOT THAW. It is recommended that patient discontinues proton pump inhibitor drugs like Omeprazole, Esomeprazole, Pantoprazole, Sample by Mon / Chemiluminescent R020 GASTRIN Lansoprazole & Rabeprazole for 7 days prior to Thu 4 pm; Immunoassay sampling as these drugs may affect test results. ReportSame day Discontinuation should be with prior consent from the treating Physician. Overnight fasting is mandatory. 2 mL (1 mL min.) serum from 1 SST for each timed Refer to Y codes: specimen. Draw baseline specimen; administer Y058 BasalY059 Secretin 2 U/kg body weight IV as a bolus injection, 5 min.Y060 10 GASTRIN and draw additional post secretin specimens at 5, min.Y061 15 Sample by Mon / Chemiluminescent Z061 STIMULATION 10, 15, 20, 25 and 30 minutes for Gastrin. Ship min.Y062 20 Thu 4 pm; Immunoassay BY SECRETIN Frozen. DO NOT THAW. All containers must be min.Y122 25 ReportSame day clearly marked with time drawn and submitted with min.Y123 30 one test request form. Overnight fasting is min.Y063 mandatory Customized GAUCHER 10 mL (7.5 mL min.) whole blood in 3 Lavender Top This tests the Sample Daily by 4 DISEASE, (EDTA) OR Green Top (Sodium heparin) tubes. Ship deficiency of G125 Enzyme assay pm; Report 4 QUANTITATIVE, refrigerated. DO NOT FREEZE.Clinical details must enzyme Beta Days BLOOD accompany sample. Glucosidase.

PAGE 173 OF 399 GENETIC Submit 3 mL (2 mL min.) whole blood in 1 Lavender Genetic counselling Sample Daily by 9 MAPPING FOR top (EDTA) tube. Ship refrigerated. DO NOT FREEZE. SNP Genotyping N076 will be providedwith am; Report 8 WHOLE Consent & Clinical Information forms available from using Microarray prior appointment. weeks GENOME LPL are mandatory.

GENETIC Submit 3 mL (2 mL min.) whole blood in 1 Lavender Genetic counselling Sample Daily by 9 MAPPING FOR top (EDTA) tube. Ship refrigerated. DO NOT FREEZE. SNP Genotyping N077 will be providedwith am; Report 8 ONCOLOGY / Consent & Clinical Information forms available from using Microarray prior appointment. weeks CANCER LPL are mandatory. Patient & Relative to report to National Reference GENETIC G173 Laboratory, Rohini, New Delhi OR Telephonically Counselling Daily COUNSELLING with prior appointment. GENETIC Submit 3 mL (2 mL min.) whole blood in 1 Lavender Genetic counselling Sample Daily by 9 MAPPING FOR top (EDTA) tube. Ship refrigerated. DO NOT FREEZE. SNP Genotyping N078 will be providedwith am; Report 8 CARDIOVASCUL Consent & Clinical Information forms available from using Microarray prior appointment. weeks ARDISORDERS LPL are mandatory.

GENETIC Submit 3 mL (2 mL min.) whole blood in 1 Lavender Genetic counselling Sample Daily by 9 MAPPING FOR top (EDTA) tube. Ship refrigerated. DO NOT FREEZE. SNP Genotyping N079 will be providedwith am; Report 8 GYNECOLOGICA Consent & Clinical Information forms available from using Microarray prior appointment. weeks LDISORDERS LPL are mandatory.

6 mL (3 mL min.) first morning Sputum OR 6 mL (3 mL min) concentrated sediments prepared from *Genexpert with induced or expectorated Sputum OR 6 mL (3 mL Sample Daily by Rifampicin min) BAL fluid OR 2 mL (1 mL min.) CSF OR 6 mL (3 Real Time PCR, 3.30 pm;Report: GENEXPERT.CO Blood & Swabs are Z518 resistance * mL min) Body fluids / Aspirates OR 2 mL (1 mL min.) Automated Interim:1 day, 14 M not acceptable Culture AFB, Pus in a sterile screw capped container OR Fresh fluorescent, ICT days & 4 weeks Rapid tissue biopsies including endometrial aspirate / Final: 6 weeks curettage in sterile normal saline Ship refrigerated. DO NOT FREEZE.

PAGE 174 OF 399 3 mL (1 mL min.) Sputum OR 3 mL (1 mL min) GENEXPERT concentrated sediments prepared from induced or MTB WITH expectorated Sputum OR 3 mL (1 mL min) BAL fluid Sample Daily by RIFAMPICIN OR 2 mL (1 mL min.) CSF OR 5 mL (3 mL min) Body Blood & Swabs are N061 Real Time PCR 11 am; Report RESISTANCE, fluids/ Aspirates OR Pus in a sterile screw capped not acceptable Same Day QUALITATIVE, container OR Fresh tissue biopsies including PCR endometrial aspirate / curettage in sterile normal saline. Ship refrigerated. DO NOT FREEZE.

GERM CELL *AFP * HCG, 3 mL (2 mL min.) serum from 1 SST. Ship CMIA, Z813 Daily TUMOR PANEL Total *LDH refrigerated or frozen. Spectrophotometry GFR calculation by this formula is not GFR 2 mL (1 mL min.) serum from 1 SST. Ship applicable for (GLOMERULAR Spectrophotometry, B168 refrigerated or frozen. Patient's age & sex is patient's less than Daily FILTRATION Calculation mandatory. 18 years. Race also RATE) plays an important role. GGTP (GAMMA GLUTAMYL 2 mL (1 mL min.) serum from 1 SST. Ship B034 IFCC Daily TRANSPEPTIDAS refrigerated or frozen. E)

GIEMSA STAIN, Submit Peripheral Blood / Bone Marrow OR Smears Sample Daily by 6 PERIPHERAL H211 fixed in absolute alcohol for 30 minutes and air Microscopy pm; Report BLOOD / BONE dried. Ship refrigerated. NextDay MARROW

Submit Body fluids/Pus/Exudates/ FNA aspirates OR Sample Daily by 6 GIEMSA STAIN, P211 Smears fixed in absolute alcohol for 30 minutes and Microscopy pm; Report BODY FLUIDS air dried. Ship refrigerated. NextDay

PAGE 175 OF 399 GLIADIN Sample by Thu 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S158 DEAMIDATED am; Report Same refrigerated or frozen. Immunoassay ANTIBODY, IgA day GLIADIN DEAMIDATED Sample by Thu 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z204 ANTIBODIES am; Report Same refrigerated or frozen. Immunoassay PANEL, IgA & day IgG GLIADIN Sample by Thu 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S159 DEAMIDATED am; Report Same refrigerated or frozen. Immunoassay ANTIBODY, IgG day

GLOMERULAR BASEMENT Sample Daily by 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence S098 MEMBRANE 11 am; Report refrigerated or frozen. Overnight fasting is preferred. Assay (GBM) Same Day ANTIBODY, IFA

3 mL (1.5 mL min.) plasma from 1 Lavender Top Sample by 7th of (EDTA) tube. Separate plasma in a plastic transport the month; RW081 GLUCAGON Extraction, RIA vial & freeze immediately. Ship frozen. DO NOT Reportafter 2–3 THAW. Overnight fasting is mandatory. weeks 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium GLUCOSE, B001 Fluoride) tube. Overnight fasting is mandatory. Ship Hexokinase Daily FASTING (F) refrigerated or frozen.

PAGE 176 OF 399 As per American 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium Diabetes Association Fluoride) tube AND 10 mL (5 mL min.) aliquot of (ADA) & WHO corresponding urine for each timed specimen. recommended test Collect fasting baseline plasma and urine specimen for diagnosis of (0 hr). Dissolve 75g Glucose in adults / 1.75 g / Kg Diabetes Mellitus is GLUCOSE body weight in children (not exceeding 75g) in Hexokinase, Strip G010. For pregnant G001 ESTIMATION, 300mL water. Administer orally over a period of 5 Daily test patient use test 2.5 HOURS minutes. Collect additional plasma and urine code G009. Also see samples at 0.5, 1.0, 1.5, 2.0 & 2.5 hours. Note time INSULIN & C- drawn on each tube, urine vial and test request PEPTIDE TESTS. form. Specify amount of glucose administered on Refer TO Y CODES: test request form. Overnight fasting mandatory. Y064 BasalY065 Ship refrigerated or frozen. 0.5 hrY066 1.0 hrÇ

As per American 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium Diabetes Association Fluoride) tube AND 10 mL (5 mL min.) aliquot of (ADA) & WHO corresponding urine for each timed specimen. recommended test Collect fasting baseline plasma and urine specimens for diagnosis of (0 hr). Dissolve 75g Glucose in adults / 1.75 g / Kg GLUCOSE Diabetes Mellitus is body weight in children (not exceeding 75g) in ESTIMATION Hexokinase, Strip G010. Also see G002 300mL water. Administer orally over a period of 5 Daily EXTENDED, 5 test INSULIN & C- minutes. Collect additional plasma and urine HOURS PEPTIDE TESTS. samples at 0.5, 1.0, 1.5, 2.0, 2.5. 3.0, 4.0 and 5.0 Refer to Y CODES: hours. Note time drawn on each tube, urine vial Y064 BasalY065 and test request form. Specify amount of glucose 0.5 hrY066 1.0 administered on test request form. Overnight hrY067 1.5 hrY068 fasting mandatory . Ship refrigerated or frozen. 2.0 hrY069 Ç

PAGE 177 OF 399 Two step screening procedure to 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium Diagnose Fluoride) tube for each timed specimen. Draw GLUCOSE Gestational Diabetes baseline fasting specimen. Dissolve 100 g of glucose TOLERANCE as Recommended in 300 ml water.Administer orally over a period of 5 B196 TEST (GTT), Hexokinase by American Daily minutes. Draw additional specimens at 1.0 , 2.0 & PREGNANCY, Diabetes 3.0 hrs. Note time drawn on each container label 100g GLUCOSE AssociationRefer to and test request form. Overnight fasting is Y CODES: Y124 mandatory. Ship refrigerated or frozen. Basal Y125 1 hrY126 2 hrs

Procedure to GLUCOSE Diagnose 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium CHALLENGE Gestational Diabetes Fluoride) tube drawn 2 hours after oral B195 TEST (GCT), Hexokinase as Recommended Daily administration of 75 g glucose. Overnight fasting is PREGNANCY, by Diabetes in NOT mandatory. Ship refrigerated or frozen. 75G GLUCOSE Pregnancy Study Group India (DIPSI)

PAGE 178 OF 399 This is the recommended test for pregnant 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium patients. If results Fluoride) tube for each timed specimen. Draw are normal in a GLUCOSE baseline fasting specimen. Dissolve 75g Glucose clinically suspected TOLERANCE in300 mL water. Administer orally over a period of 5 case of Gestational G009 TEST (GTT), Hexokinase Daily minutes. Draw additional specimens at 1.0 & 2.0 Diabetes mellitus, a PREGNANCY, hours. Note time drawn on each container label repeat test in third 75g GLUCOSE and test request form. Overnight fasting trimester is mandatory. Ship refrigerated or frozen. recommended. Refer to Y CODES: Y124 Basal Y125 1 hrY126 2 hrs

2 mL (1 mL min.) plasma each for Fasting and Post GLUCOSE, Prandial specimens from 1 Grey Top (Sodium FASTING (F) Fluoride) tube. Overnight fasting mandatory. Z131 AND POST Collect fasting specimen. Ask patient to have a Hexokinase Daily MEAL (PP), 2 normal to heavy meal. Note the time patient starts HOURS the meal. Collect PP sample exactly after 2 hours. Ship refrigerated or frozen 2 mL (1 mL min.) Body fluid in a sterile screw GLUCOSE, P085 capped container. Ship refrigerated. DO NOT Hexokinase Daily FLUID FREEZE.

PAGE 179 OF 399 Also see GLUCOSE TOLERANCE TEST. Use the following test codes if glucose is administered OR GLUCOSE, 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium PP time is POST B002 Fluoride) tube. Collect sample exactly 2 hours after Hexokinase altered.Code Daily PRANDIAL (PP), starting meal. Ship refrigerated or frozen. Meal/GlucoseB187 2 HOURS 0.5 Hr after 75 gB143 1.0 Hr after 75 gB006 1.5 Hrs afteÇ GLUCOSE CHALLENGE 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium TEST (GCT); Fluoride) tube drawn 1 hour after oral G004 Hexokinase Daily GLUCOSE 1 administration of 50 g glucose. Does not include a HOUR fasting specimen . Ship refrigerated or frozen. OBSTETRICAL

2 mL (1 mL min.) plasma from 1 Grey Top (Sodium Fluoride) tube each for Fasting and Post glucose As per American specimens. Collect fasting specimen. Dissolve 75g GLUCOSE Diabetes Association Glucose in adults / 1.75 g / Kg body weight in TOLERANCE (ADA) & WHO this is G010 children (not exceeding 75g) in 300mL water. Hexokinase Daily TEST, ORAL the recommended Administer orally over a period of 5 minutes. Collect (OGTT) test for diagnosis of post glucose sample exactly after 2 hours. Overnight Diabetes Mellitus. fasting is mandatory. Ship refrigerated or frozen. Urine Specimens NOT REQUIRED.

GLUCOSE, 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium B003 Hexokinase Daily RANDOM (R) Fluoride) tube. Ship refrigerated or frozen.

PAGE 180 OF 399 GLUTATHIONE- S- Sample Daily by 9 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS142 TRANSFERASE PCR am; Report 29 (EDTA) tube. Ship refrigerated. DO NOT FREEZE. MUTATION days DETECTION The test can help to GLYCEMARK identify patients Sample by (1,5 - 2 mL (1 mL min.) serum from 1 SST. Ship Enzymatic who may need B198 Mon/Thu 9 am; ANHYDROGLUCI refrigerated or frozen. Colorimetric therapy changes Report same day TOL) SERUM despite a “good” HbA1c GM1 10 ml (7.5 mL min.) whole blood from 3 Lavender GANGLIOSIDOSI This tests deficiency Sample Daily by 4 Top (EDTA) / Green Top (Sodium heparin) tubes. G123 S, Enzyme Assay of enzyme Beta pm; Report 4 Ship refrigerated. DO NOT FREEZE. Clinical details QUANTITATIVE, Galactosidase. Days must accompany sample. BLOOD GM2 GANGLIOSIDOSI S, 10 ml (7.5 mL min.) whole blood from 3 Lavender This tests deficiency Sample Daily by 4 QUANTITATIVE, Top (EDTA) / Green Top (Sodium heparin) tubes. of enzyme Total G124 Enzyme Assay pm; Report 4 BLOOD; TAY Ship refrigerated. DO NOT FREEZE. Clinical details Hexosaminidase A & days SACHS AND must accompany sample. B. SANDHOFFDISE ASE *Glomerular GOODPASTURE’ Basement Sample by Mon / 3 ml (1.5 mL min.) serum from 1 SST. Ship Enzyme Z200 S SYNDROME Membrane Wed 9 am; refrigerated or frozen. Immunoassay PANEL Antibody*ANCA- ReportNext day PR3 / c-ANCA G6PD GENE 4 mL (2 mL min.)Whole Blood in 1 Lavender top Sample by Tue 11 OS139 MUTATION PCR (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. am; Report 8 days DETECTION

PAGE 181 OF 399 1 drop of heel prick blood each on 3 spots of filter G-6-PD, Sample by Tue / paper available from LPL. Clinical details and drug R158 NEWBORN Fluoroimmunoassay Thu / Sat 5 history must accompany sample. Ship refrigerated SCREEN pm;Report 2 days or frozen. Sample Mon G-6-PD, 3 mL (2 mL min.) whole blood in 1 Lavender Top through Sat by 4 H018 Spectrophotometry QUANTITATIVE (EDTA) tube. Ship refrigerated. DO NOT FREEZE. pm;Report Same day Submit specimens in a sterile screw capped Microscopy of Gram P025 GRAM STAIN container OR smeared onto 2 sterile glass slides in a Daily stained smears slide mailer.Ship refrigerated. GROUP A Sample Daily 1 Throat Swab in a sterile screw capped container. M214 STREPTOCOCCA ICT pmReport Same Ship refrigerated only. DO NOT FREEZE. L ANTIGEN day Patient should be at 2 mL (1 mL min.) serum from 1 SST for each timed rest for 30 minutes specimen. Draw baseline specimen after an prior to basal GROWTH overnight fast. Administer Glucose orally 1.75 g/kg sample HORMONE body weight (maximum 75g). Draw additional Chemiluminescent Z069 collectionrefer to Y Daily SUPPRESSION specimens at 60 & 120 min for GH. Ship refrigerated Immunoassay Codes:Y100 Basal BY GLUCOSE or frozen. All containers must be clearly marked Y102 60 min.Y104 with time drawn and submitted with 1 test request 120 min. Y105 form. Customized

PAGE 182 OF 399 Patient should be at rest for 30 minutes prior to sample collection. Also see GROWTH 2 mL (1 mL min.) serum from 1 SST. Ship GROWTH Chemiluminescent HORMONE R031 refrigerated or frozen. Overnight fasting is Daily HORMONE; GH Immunoassay STIMULATION TEST, mandatory. GROWTH HORMONE SUPPRESSION TEST & GROWTH DISORDER PANEL. Sample by 7th of GROWTH 2 mL (1 mL min.) serum from 1 Red Top (No the month; RW082 HORMONE Radiobinding Assay Additive) tube. Ship refrigerated or frozen. Reportafter 2–3 ANTIBODY weeks Patient should be at GROWTH *Growth Sample by Mon / 3 mL (1.5 mL min.) serum from 1 SST. Ship frozen. Chemiluminescent rest for 30 minutes Z208 DISORDER Hormone Thu 4 pm; DO NOT THAW. Overnight fasting is mandatory. Immunoassay prior to sample PANEL *IGFBP-3 *IGF-I ReportSame day collection. GROWTH 3 mL (1 mL min.) serum from 1 Red Top (No Patient to avoid all Sample by 7th of HORMONE Additive) tube. Separate serum into a sterile screw Extraction, medications that the month; RW083 RELEASINGHOR capped vial and freeze immediately. Ship frozen DO Radioimmunoassay may influence Reportafter 2–3 MONE; GHRH NOT THAW. pituitary secretion. weeks

PAGE 183 OF 399 2 mL (1 mL min.) serum from 1 SST for each timed specimen. Draw baseline specimen after an Patient should be at overnight fast. Administer stimulating agent rest for 30 minutes Clonidine (Arkamine) 300 ug OR 5ug / Kg body prior to basal weight orally. Draw additional specimens at 30, 60 GROWTH sample & 90 mins. for Growth Hormone. Ship refrigerated HORMONE Chemiluminescent collection.Refer to Y Z300 or frozen. All containers must be clearly marked Daily STIMULATION Immunoassay Codes:Y094 Basal with time drawn and submitted with one test TEST Y095 30 min. Y096 request form.Other stimulating agents that can be 60 min. Y097 90 used are:Insulin (0.10 - 0.15 U / Kg body weight IV min. Y098 followed by additional samples at 30, 60 & 90 Customized min).Exercise (20 min. vigorous exercise followed by immediate sampling) HAINS MYCOBACTERIU M TUBERCULOSIS Submit 3mL (2mL min.) Sputum / bronchoalveolar PCR, Line probe Sample by Sat 11 Z835 FIRST & lavages / bronchial aspirates / Culture growth in a assay am, ReportFri SECOND LINE sterile screw capped container. DRUG RESISTANCE PROFILE HAINS MYCOBACTERIU Submit 2 mL (1 mL min) Sputum / Bronchoalveolar M Lavage / Pleural aspirates / CSF / Body fluid OR Sample by Tue / PCR, Line probe N068 TUBERCULOSISF Cultured growth on appropriate media in a sterile Fri 11 am, assay IRST LINE DRUG screw capped container in a sealed plastic bag. Ship ReportThu / Mon RESISTANCE refrigerated. DO NOT FREEZE PROFILE

PAGE 184 OF 399 HAINS MYCOBACTERIU M Submit 3mL (2mL min.) Sputum / bronchoalveolar TUBERCULOSISS PCR, Line probe Sample by Sat 11 N117 lavages / bronchial aspirates / Culture growth in a ECOND LINE assay am, ReportFri sterile screw capped container. DRUG RESISTANCE PROFILE 2 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th of Additive) tube. Do not use SST gel barrier tubes. the month; RW084 HALOPERIDOL LC-MS/MS Collect specimen 11–17 hours after the last dose. Reportafter 2–3 Ship refrigerated or frozen. weeks HAM TEST; Also see CD 55 & CD PAROXYSMAL 59 - Paroxysmal NOCTURNALHE Sample Daily by 9 3 mL (2 mL min.) whole blood from 1 Lavender Top Nocturnal H119 MOGLOBINURIA Gel Technique am; Report Same (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Hemoglobinuria (PNH), Day confirmatory test by SCREENING Flow Cytometry. TEST Sample by HAPTOGLOBIN 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS144 PCR Wed/Sat 11 am; GENOTYPING (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. Report 6 days 2 mL (1 mL min.) serum from 1 SST. Overnight B100 HAPTOGLOBIN Immunoturbidimetry Daily fasting is preferred. Ship refrigerated or frozen. Also see HbA1c; High Performance FRUCTOSAMINE, 3 mL (2 mL min.) whole blood in 1 Lavender Top B080 GLYCOSYLATED LiquidChromatograp LIPID PROFILE & Daily (EDTA) tube. Ship refrigerated. DO NOT FREEZE. HEMOGLOBIN hy MICROALBUMINURI A.

PAGE 185 OF 399 HBDH; ALPHA - HYDROXYBUTYR 2 mL (1 mL min.) serum from 1 SST. Ship B030 GSCC Daily ATEDEHYDROG refrigerated or frozen. ENASE HCG, BETA, TOTAL, Chemiluminescent 2 mL (1 mL min.) serum from 1 SST. Ship F002 QUANTITATIVE, MicroparticleImmun Daily refrigerated or frozen. TUMOR oassay MARKER HCG, BETA, Chemiluminescent Also see MATERNAL TOTAL, 2 mL (1 mL min.) serum from 1 SST. Ship R021 MicroparticleImmun SERUM SCREEN 3 & Daily QUANTITATIVE, refrigerated or frozen. oassay 4. MATERNAL HCG, BETA, TOTAL, 10 mL (5 mL min.) aliquot of random urine in a Chemiluminescent U075 QUANTITATIVE, sterile screw capped container. Ship refrigerated or MicroparticleImmun Daily RANDOM frozen. oassay URINE HCG, BETA, 10 mL (5 mL min.) aliquot of 24-hour urine in a Chemiluminescent TOTAL, U022 sterile screw capped container. Ship refrigerated or MicroparticleImmun Daily QUANTITATIVE, frozen. oassay 24 HOUR URINE Sample by 7th of HDL 4 mL (2 mL min.) serum from 1 Red Top (No Precipitation, the month; RW085 CHOLESTEROL *HDL-2 *HDL-3 Additive) tube. Minimum 12 hours overnight fasting Colorimetric, Reportafter 2–3 SUBCLASSES is mandatory. Ship refrigerated or frozen Enzymatic weeks HDL 2 mL (0.5 mL min.) serum from 1 SST. Ship Enzymatic Also see LIPID B128 CHOLESTEROL, Daily refrigerated or frozen. Immunoinhibition PROFILE TESTS. DIRECT

PAGE 186 OF 399 *hsCRP *Uric 3 mL (2 mL min.) serum from 1 SST AND 2 mL (1 mL Immunoturbidimetry acid *Glucose min.) plasma from 1 Grey Top (Sodium Fluoride) , Z615 HEART SCREEN Fasting *Lipid Daily tube. Ship refrigerated or frozen. Minimum 12 Hexokinase,Spectrop profile basic hours fasting is mandatory. hotometry, CMIA *Homocysteine

HE4; HUMAN Also see ROMA (Risk Sample by Mon / 2 mL (1 mL min.) serum from 1 SST. Ship F024 EPIDIDYMIS CMIA of Ovarian Thu 4 pm; refrigerated or frozen. PROTEIN 4 Malignancy) ReportSame day

HEINZ BODIES, 2 air dried peripheral blood smears. Ship in a slide H204 PERIPHERAL mailer at room temperature. Give brief clinical Microscopy Daily BLOOD history. HELICOBACTER PYLORI Giemsa staining for Sample by Thu 9 3 mL (1.5 mL min.) serum from 1 SST. Ship Enzyme Z121 ANTIBODIES H. pylori also am; Report Same refrigerated or frozen. Immunoassay PANEL, IgA & available. day IgG Giemsa staining for Sample by Thu 9 HELICOBACTER 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S082 H. pylori also am; Report Same PYLORI, IgG refrigerated or frozen. Immunoassay available. day Giemsa staining for Sample by Thu 9 HELICOBACTER 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S083 H. pylori also am; Report Same PYLORI, IgA refrigerated or frozen. Immunoassay available. day HELICOBACTER 5g (3g min.) stool in a leakproof screw capped Sample by Mon 9 PYLORI Enzyme S227 container. Do not use matchbox or absorbent am; Report Same ANTIGEN, Immunoassay material. Ship refrigerated or frozen. day STOOL HEMOCHROMA Sample Daily by 9 TOSIS 2 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS143 PCR am; Report 32 MUTATION (EDTA) tube. Ship refrigerated. DO NOT FREEZE. days DETECTION

PAGE 187 OF 399 HEMOGLOBIN Sample by Tue/ D IRAN 4 mL (2 mL min.)Whole Blood in 1 Lavender top OS146 PCR, Sequencing Thu 9 am; Report MUTATION (EDTA) tube. Ship refrigerated. DO NOT FREEZE. 8 days ANALYSIS HEMOGLOBIN Sample by Tue/ E (GLU 26LYS) 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS148 PCR, Sequencing Thu 9 am; Report MUTATION (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. 8 days ANALYSIS HEMOGLOBIN; 3 mL (1 mL min.) whole blood in 1 Lavender Top H001 Photometry Daily Hb (EDTA) tube. Ship refrigerated. DO NOT FREEZE. HEMOGLOBIN Sample by Tue/ D PUNJAB 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS147 PCR, Sequencing Thu 9 am; Report MUTATION (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. 8 days ANALYSIS HPLC is the method of choice. Hemoglobin electrophoresis 4 mL (2 mL min.) whole blood from 1 Lavender Top (Alkaline pH) on HEMOGLOBIN Includes High Performance Sample Daily by (EDTA) tube. Ship refrigerated. DO NOT FREEZE. cellulose acetate will E002 HPLC/ELECTROP Hematological LiquidChromatograp 10 am; Report Must Record date of birth, sex, race and religion on be carried out, if HORESIS Indices. hy/ Electrophoresis Same Day the test request form. necessary, in selected cases, at no additional charge and reported after 3 days. HEMOGLOBIN, 10 mL (5 mL min.) aliquot of random urine. Ship U047 Strip Test Daily FREE, URINE refrigerated.

PAGE 188 OF 399 *Hemoglobin *PCV *TLC *RBC Count *MCV*MCH Electrical *MCHC *Platelet 4 mL (2 mL min.) whole blood in 1 Lavender Top Impedence, VCS, Peripheral smear Z019 HEMOGRAM Count (EDTA) tube. Ship refrigerated. DO NOT Capillary reported in Daily *Automated FREEZE.Overnight fasting is preferred. Photometry / abnormal cases only DLC*Absolute Westergren Differential counts *RDW *ESR

3 mL Whole blood in 1 Blue Top(Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hrs. If this is not possible, make PPP within 1 hour of collection as follows: Centrifuge sample at *Factor VIII HEMOPHILIA A 3600 rpm for 15 min & transfer supernatant to a Sample by 1st & *vWF Antigen * Electromechanical H241 CARRIER clean plastic tube. Centrifuge this supernatant again 3rd Mon 11 am; Factor VIII / vWF Clot Detection, EIA DETECTION at 3600 rpm for 15 mins & finally transfer the Report Next day Ratio supernatant (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form is mandatory.

PAGE 189 OF 399 It is recommended that patient discontinues 3 mL Whole blood in 1 Blue Top (Sodium Citrate) Heparin for 1 day tube. Mix thoroughly by inversion. Transport to Lab and Oral within 4 hrs. If this is not possible, make PPP within Anticoagulants for 7 1 hour of collection as follows: Centrifuge sample at days prior to 3600 rpm for 15 min & transfer supernatant to a Photo-optical & sampling as these Sample by Wed / HEMOPHILIA *APTT *Factor Z374 clean plastic tube. Centrifuge this supernatant again Electromechanical drugs may affect Sat 9 am; Report PANEL VIII *Factor IX at 3600 rpm for 15 mins & finally transfer the Clot Detection test results. Fri / Tue supernatant (PPP) to 1 labelled, clean plastic screw Discontinuation capped vial. FREEZE IMMEDIATELY. Ship frozen. DO should be with prior NOT THAW. Overnight fasting is preferred. Duly consent from the filled Coagulation Requisition Form is mandatory. treating Physician. Also see COAGULATION PROFIÇ

10 mL (5 mL min.) aliquot of random urine. First morning specimen is preferred. Ship refrigerated. Sample Daily by 9 HEMOSIDERIN, DO NOT FREEZE OR Centrifuge urine at 3200 rpm Prussian Blue U046 am; Report Same URINE for 15 min. Smear deposit on 4 glass slides, air dry, Reaction day fix in 95% ethanol for 30 min. Ship at room temperature.

PAGE 190 OF 399 2 mL (1 mL min.) platelet poor plasma from 2 Blue Top (Sodium Citrate) tubes. Make PPP within 1 hour of collection as follows: Centrifuge sample at 3600 Sample by 7th of rpm for 15 min & transfer supernatent to a clean HEPARIN, ANTI the month; RW086 plastic tube. Centrifuge this supernatant again at Chromogenic Xa ASSAY Reportafter 2–3 3600 rpm for 15 mins & finally transfer the weeks supernatant (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. Overnight fasting is preferred. *Anti-HAV IgM *HBsAg *HBe Ag Sample by Mon HEPATITIS Chemiluminescent * Anti-HBe*Anti- 4 mL (2 mL min.) serum from 2 SST's. Ship through Sat 9 Z372 ACUTE VIRUS MicroparticleImmun HBc IgM *Anti- refrigerated or frozen. am; Report Same CONFIRMATION oassay, EIA HCV *Anti-HEV day IgM HEPATITIS B Chemiluminescent SURFACE 2 mL (1 mL min.) serum from 1 SST. Ship S057 MicroparticleImmun Daily ANTIBODY; refrigerated or frozen. oassay ANTI HBs HEPATITIS B 2 mL (1 mL min.) serum from 1 SST. Indicate on test SURFACE Chemiluminescent request form that specimen has been found positive S116 ANTIGEN MicroparticleImmun Daily and that a confirmation procedure isneeded. Ship (HBsAg) oassay refrigerated or frozen. CONFIRMATION

HEPATITIS B SURFACE Chemiluminescent 2 mL (1 mL min.) serum from 1 SST. Ship S215 ANTIGEN MicroparticleImmun Daily refrigerated or frozen. (HBsAg), oassay QUANTITATIVE

PAGE 191 OF 399 For Hepatitis B HEPATITIS B surface antigen SURFACE confirmation by Chemiluminescent ANTIGEN; 2 mL (1 mL min.) serum from 1 SST. Ship neutralization, use S056 MicroparticleImmun Daily HBsAg; refrigerated or frozen. test code S116 oassay AUSTRALIA HEPATITIS B ANTIGEN SURFACE ANTIGEN CONFIRMATION. HEPATITIS B 3 mL (2 mL min.) plasma from 2 Lavender Top The minimum viral Sample Daily by VIRAL DRUG N041 (EDTA) tubes. Separate plasma aseptically within 2 Sequencing load required is 11 am; Report 7 RESISTANCE & hours of collection. Ship refrigerated or frozen. 2000 IU/mL days GENOTYPING HEPATITIS B 2 mL (1 mL min.) plasma from 1 Lavender Top Sample by Tue / VIRUS, PCR, (EDTA) tube. Separate plasma aseptically within 2 Polymerase Chain Thu / Sat 11 am; N011 QUALITATIVE;H hours of collection. Ship refrigerated or frozen. Reaction Report Wed / Fri BV DNA PCR, Overnight fasting is recommended. / Mon QUALITATIVE HEPATITIS Be Chemiluminescent 2 mL (1 mL min.) serum from 1 SST. Ship S059 ANTIGEN; MicroparticleImmun Daily refrigerated or frozen. HBeAg oassay

Chemiluminescent HEPATITIS Be * Anti-HBe * 2 mL (1 mL min.) serum from 1 SST. Ship Z137 MicroparticleImmun Daily PANEL HBeAg refrigerated or frozen. oassay

HEPATITIS C Chemiluminescent 2 mL (1 mL min.) serum from 1 SST. Ship S062 ANTIBODY MicroparticleImmun Daily refrigerated or frozen. (Anti-HCV) oassay

PAGE 192 OF 399 HEPATITIS C 3 mL (2 mL min.) serum from 2 SST's OR 3 mL (2 mL VIRAL RNA min.) plasma from 2 Lavender Top (EDTA) tubes. Sample by Tue / Avoid freeze thaw N028 (HCV RNA) Separate serum/plasma aseptically within 2 hours of Real Time PCR Fri 11 am; cycles. QUANTITATIVE, collection. 12 hours fasting is recommended. Ship ReportThu / Mon REAL TIME PCR refrigerated or frozen. 2 mL (1 mL min.) serum from 1 SST OR 2 mL (1 mL HEPATITIS C min.) plasma from 1 Lavender Top (EDTA) tube. Sample by Tue / N032 VIRAL RNA, Separate serum/plasma aseptically within 1 hour of Real Time PCR Fri 11 am; GENOTYPE collection. 12 hours fasting is recommended. Ship ReportThu / Mon refrigerated or frozen. Sample by Mon HEPATITIS E 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme through Sat 9 S051 ANTIBODY refrigerated or frozen. Immunoassay am; Report Same (Anti-HEV), IgM day HEPATITIS E 2 ml (1 ml min.) serum from 1 SST OR 2 ml (1 ml Sample by Mon / VIRUS min.) plasma from 1 Lavender top (EDTA) tube. N109 Real Time PCR Thu11 am; DETECTION, Separate plasma / serum within 4 hours of Report Wed / Sat PCR collection. Ship refrigerated or frozen. HEPATITIS G 4 mL (2 mL min.) plasma from 1 Lavender Top Sample by Thu VIRUS RNA (EDTA) tube. Separate plasma aseptically within 2 OS150 PCR 11 am; Report DETECTION , hours of collection. Ship refrigerated or frozen. 12 Wed PCR hours fasting is recommended. *Anti-HAV, IgG *Anti-HAV IgM HEPATITIS A & *Anti-HBc Chemiluminescent 4 mL (2 mL min.) serum from 2 SST's. Ship Z365 B VIRUS Total*Anti-HBc MicroparticleImmun Daily refrigerated or frozen. EVALUATION IgM *Anti-HBs oassay *HBsAg *Anti- HBe*HBeAg HEPATITIS *Anti-HAV IgM Chemiluminescent ACUTE 2 mL (1 mL min.) serum from 1 SST. Ship Z133 *HBsAg *Anti-HB MicroparticleImmun Daily DIAGNOSTIC refrigerated or frozen. Core IgM oassay PANEL

PAGE 193 OF 399 *Anti-HAV IgM Sample by Mon HEPATITIS *HBsAg *Anti-HB 2 mL (1 mL min.) serum from 1 SST. Ship through Sat 9 Z371 ACUTE VIRUS Core IgM*Anti- CMIA, EIA refrigerated or frozen. am; Report Same SCREEN HCV *Anti-HEV day IgM

HEPATITIS *CMV IgM *HSV Sample by Mon ATYPICAL IgM *Varicella 4 mL (2 mL min.) serum from 2 SST's. Ship / Thu 9 am; Z373 CMIA, EIA VIRUS Zoster IgM*EBV refrigerated or frozen. Report Thu / CONFIRMATION (VCA), IgM Mon

HEPATITIS B Chemiluminescent CORE 2 mL (1 mL min.) serum from 1 SST. Ship S058 MicroparticleImmun Daily ANTIBODY refrigerated or frozen. oassay (Anti-HBc), IgM HEPATITIS A 4 mL (2 mL min.) plasma from 1 Lavender Top VIRUS RNA (EDTA) tube. Separate plasma aseptically within 2 Sample by Fri 11 OS145 PCR DETECTION, hours of collection. Ship refrigerated or frozen. 12 am; Report Wed PCR hours fasting is recommended. *HBsAg *Anti- HBs *HBeAg Chemiluminescent HEPATITIS B 4 mL (2 mL min.) serum from 2 SST’s. Ship Z017 *Anti-HBe *Anti- MicroparticleImmun Daily PROFILE refrigerated or frozen. HBc IgM *Anti- oassay HBc Total HEPATITIS B VIRUS 3 mL (2 mL min.) plasma from 2 Lavender Top GENOTYPING, Sample Daily by (EDTA) tubes. Separate plasma asceptically within 2 Sequencing, Real Avoid freeze thaw Z602 DRUGRESISTAN 11 am; Report 7 hours of collection. Ship refrigerated or frozen. 12 Time PCR cycles CE & days hours fasting is recommended QUANTITATIVE PCR

PAGE 194 OF 399 6 mL (4 mL min.) serum from 2 SSTs OR 6 mL (4 mL This test is not *HCV RNA min.) plasma from 2 Lavender Top (EDTA) tubes. intended for use in Sample by Tue / HEPATITIS C Quantitative Z511 Separate serum/plasma aseptically within 2 hours of Real Time PCR the initial diagnosis Fri 11 am; VIRAL COMBO Real Time PCR * collection. Ship refrigerated or frozen. 12 hours or confirmation of ReportThu / Mon HCV Genotype fasting is recommended. HCV infection.

HEPATITIS E Sample by Mon / 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S202 ANTIBODY Wed / Fri 9 am; refrigerated or frozen. Immunoassay (Anti-HEV), IgG Report Same day *Anti-HAV, IgG *Anti-HAV IgM *Anti-HBc HEPATITIS, Total*Anti-HBc Chemiluminescent Sample by Mon / VIRAL, IgM *Anti-HBs 6 mL (4 mL min.) serum from 2 SST’s. Ship Z138 MicroparticleImmun Wed / Fri 9 am; COMPREHENSIV *HBsAg *Anti- refrigerated or frozen. oassay, EIA Report Same day E PANEL HBe*HBe Ag *Anti-HCV *Anti- HEV IgG *Anti- HEV IgM HEPATITIS A Chemiluminescent 2 mL (1 mL min.) serum from 1 SST. Ship S090 ANTIBODY MicroparticleImmun Daily refrigerated or frozen. (Anti-HAV), IgG oassay

HEPATITIS A Chemiluminescent 2 mL (1 mL min.) serum from 1 SST. Ship S061 ANTIBODY MicroparticleImmun Daily refrigerated or frozen. (Anti-HAV), IgM oassay HEPATITIS A *Anti-HAV, IgG 2 mL (1 mL min.) serum from 1 SST. Ship Z132 DIAGNOSTIC CMIA Daily *Anti-HAV, IgM refrigerated or frozen. PANEL Chemiluminescent HEPATITIS B *HBsAg * HBeAg 2 mL (1 mL min.) serum from 1 SST. Ship Z134 Microparticle Daily CHRONIC PANEL * Anti-HBe refrigerated or frozen. Immunoassay

PAGE 195 OF 399 HEPATITIS B CORE Chemiluminescent 2 mL (1 mL min.) serum from 1 SST. Ship S115 ANTIBODY Microparticle Daily refrigerated or frozen. (Anti-HBc), Immunoassay TOTAL HEPATITIS B *Anti-HBc Total Chemiluminescent 2 mL (1 mL min.) serum from 1 SST. Ship Z135 IMMUNITY *Anti-HBs MicroparticleImmun Daily refrigerated or frozen. SCREEN *HBsAg oassay HEPATITIS B Chemiluminescent INFANT *HBsAg *Anti- 2 mL (1 mL min.) serum from 1 SST. Ship Z136 MicroparticleImmun Daily FOLLOW-UP HBs refrigerated or frozen. oassay PANEL HEPATITIS B 3 mL (2 mL min.) plasma from 2 Lavender Top Sample by Tue / VIRAL DNA (EDTA) tubes OR 2 mL (1 mL min.) serum from 1 Avoid freeze thaw Thu / Sat 11 am; N027 (HBV DNA) SST. Separate plasma / serum aseptically within 2 Real Time PCR cycles Report Wed / Fri QUANTITATIVE, hours of collection. Ship refrigerated or frozen. 12 / Mon REAL TIME PCR hours fasting is recommended.

HEPATITIS Be Chemiluminescent 2 mL (1 mL min.) serum from 1 SST. Ship S060 ANTIBODY; MicroparticleImmun Daily refrigerated or frozen. Anti-HBe oassay

HEPATITIS C 2 mL (1 mL min.) serum from 1 SST OR 2 mL (1 mL VIRAL, PCR, min.) plasma from 1 Lavender Top (EDTA) tube. Sample by Tue / Polymerase Chain Avoid freeze thaw N004 QUALITATIVE; Separate serum/plasma aseptically within 2 hours of Fri 11 am;Repot Reaction cycles. HCV RNA, PCR, collection. Ship refrigerated or frozen. Overnight Thu/ Mon QUALITATIVE fasting is recommended. HEPATITIS D 4 mL (2 mL min.) plasma from 1 Lavender Top Sample by Thu VIRUS (EDTA) tube. Separate plasma aseptically within 2 OS149 PCR 11 am; Report DETECTION, hours of collection. Ship refrigerated or frozen. 12 Wed PCR hours fasting is recommended.

PAGE 196 OF 399 HEPATITIS Sample by Wed DELTA 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S117 11 am; Report ANTIBODY refrigerated or frozen. Immunoassay Same Day (Anti-HDV), IgM

HERPES SIMPLEX VIRUS 2 mL (1 mL min.) serum from 1 SST. Ship S021 CLIA Daily (HSV) 1+2, IgG, refrigerated or frozen. SERUM

HERPES SIMPLEX VIRUS 2 mL (1 mL min.) serum from 1 SST. Ship S071 CLIA Daily (HSV) 1+2, IgM, refrigerated or frozen. SERUM

For detection of HERPES Acute HSV Sample by Tue / SIMPLEX VIRUS 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S214 infections, HSV PCR Fri 9 am; Report (HSV) 2 IgM, refrigerated or frozen. Immunoassay is the recommended Same Day SERUM test. HERPES For detection of SIMPLEX VIRUS Enzyme Acute HSV Sample by Tue / (HSV) 1 3 mL (2 mL min.) serum from 1 SST. Ship Immunoassay Z556 infections, HSV PCR Fri 9 am; Report ANTIBODIES refrigerated or frozen. Chemiliminescent is the recommended Same Day PANEL, IgG & Immunoassay test. IgM, SERUM For detection of HERPES Acute HSV Sample by Tue / SIMPLEX VIRUS 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent S211 infections, HSV PCR Fri 9 am; Report (HSV) 1 IgG, refrigerated or frozen. Immunoassay is the recommended Same Day SERUM test.

PAGE 197 OF 399 For detection of HERPES Acute HSV Sample by Tue / SIMPLEX VIRUS 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S213 infections, HSV PCR Fri 9 am; Report (HSV) 1 IgM, refrigerated or frozen. Immunoassay is the recommended Same Day SERUM test. HERPES For detection of SIMPLEX VIRUS Acute HSV Sample by Tue / (HSV) 2 3 mL (2 mL min.) serum from 1 SST. Ship Chemiluminescent Z557 infections, HSV PCR Fri 9 am; Report ANTIBODIES refrigerated or frozen. Immunoassay is the recommended Same Day PANEL, IgG & test. IgM, SERUM For detection of HERPES Acute HSV Sample by Tue / SIMPLEX VIRUS 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent S212 infections, HSV PCR Fri 9 am; Report (HSV) 2 IgG, refrigerated or frozen. Immunoassay is the recommended Same Day SERUM test.

2 mL (1 mL min.) CSF/EDTA Plasma in a sterile screw HERPES capped container OR Blister fluid/Swab from base of SIMPLEX VIRUS Polymerase Chain Sample by Mon / ulcer/ Scrapings from base of ulcer in 1 mL sterile Z303 (HSV) TYPE 1 & Reaction, Thu 11 am; normal saline or phosphate buffered saline (PBS) OR 2, PCR, Amplification ReportWed / Sat Fresh tissue in sterile normal saline OR Paraffin QUALITATIVE embedded tissue. Ship refrigerated or frozen.

2 mL (1 mL min.) CSF/EDTA Plasma in a sterile screw HERPES capped container OR Blister fluid/Swab from base of SIMPLEX VIRUS Polymerase Chain Sample by Mon / ulcer/ Scrapings from base of ulcer in 1 mL sterile N013 (HSV) TYPE 2, Reaction, Thu 11 am; normal saline or phosphate buffered saline (PBS) OR PCR, Amplification ReportWed / Sat Fresh tissue in sterile normal saline OR Paraffin QUALITATIVE embedded tissue. Ship refrigerated or frozen.

PAGE 198 OF 399 2 mL (1 mL min.) CSF/EDTA Plasma in a sterile screw HERPES capped container OR Blister fluid / Swab from base SIMPLEX VIRUS Polymerase Chain Sample by Mon / of ulcer/ Scrapings from base of ulcer in 1 mL sterile N012 (HSV) TYPE I, Reaction, Thu 11 am; normal saline or phosphate buffered saline (PBS) OR PCR, Amplification Report Wed / Sat Fresh tissue in sterile normal saline OR Paraffin QUALITATIVE embedded tissue. Ship refrigerated or frozen.

HERPES SIMPLEX VIRUS (HSV) 1+2 2 mL (1 mL min.) serum from 1 SST. Ship Z205 CLIA Daily ANTIBODIES refrigerated or frozen. PANEL, IgG & IgM, SERUM HERPES VIRUS Sample by 7th of 6 (HHV-6) 2 mL (1 mL min.) serum from 1 Red Top (No Immunofluorescence the month; RW088 ANTIBODY Additive) tube. Ship refrigerated or frozen. Assay Reportafter 2–3 PANEL, IgG & weeks IgM HHH SYNDROME; HYPERORNITHI NEMIA - 15 mL (10 mL min.) aliquot of random urine in a Sample Daily by 5 G185 HYPERAMMON sterile screw capped container. No preservative LC-MS/MS, GC-MS pm; Report 5 EMIA - required. Ship refrigerated or frozen. days HOMOCITRULLI NURIA SYNDROME

PAGE 199 OF 399 50 mL (10 mL min.) aliquot of 24-hour urine. Collect urine with appropriate volume of 50% HCl to maintain pH between 1–2. Do not use concentrated HCl. Record 24 hour volume on test request form and urine container. Ship refrigerated or frozen. 5-HIAA (5- Patient should strictly avoid banana, kiwi, walnut, HYDROXY High Performance Sample by Wed / avocado, brinjal, pineapple, plum, tomato & drugs U034 INDOLE ACETIC LiquidChromatograp Sat 9 am; Report like Fluorouracil, Melphalan, Paracetamol & ACID), 24- hy Fri / Tue Acetaminophen, Heparin, L-dopa, Reserpine, HOUR URINE Salicylates, Chlorpromazine, Imipramine, Isoniazid, MAO inhibitors, Phenothiazines, Promethazine, alcoholic beverages, caffeine, tea/coffee, tobacco and strenuous exercise at least 72 hrs before and during specimen collection.

50 mL (10 mL min.) aliquot of random urine. Add 50% HCl drop by drop to bring the pH between 1-2. Do not use concentrated HCl. Ship refrigerated or 5 - HIAA (5- frozen. Patient should strictly avoid banana, kiwi, HYDROXY walnut, avocado, brinjal, pineapple, plum, tomato & High Performance Sample by Wed / INDOLE ACETIC drugs like Fluorouracil, Melphalan, Paracetamol & U038 Liquid Sat 9 am; Report ACID), Acetaminophen, Heparin, L-dopa, Reserpine, Chromatography Fri / Tue RANDOM Salicylates, Chlorpromazine, Imipramine, Isoniazid, URINE MAO inhibitors, Phenothiazines, Promethazine, alcoholic beverages, caffeine, tea/coffee, tobacco and strenuous exercise at least 72 hrs before and during specimen collection.

*Androstenedion 5 mL (3 mL min.) serum from 2 SST’s. An early Sample by Mon HIRSUTISM e *DHEA Z040 morning specimen is preferred. Ship refrigerated or CLIA, CMIA, RIA / Thu 9 am; PANEL Sulphate *Free frozen. Specify age and sex on test request form. Report Same Day Testosterone

PAGE 200 OF 399 5 mL (3 mL min.) aliquot of 24-hour urine. Collect urine with 10 mL of 50% 6N HCl to maintain pH Sample by 7th of between 1–3. Record 24 hour volume on test HISTAMINE, 24- the month; RW089 request form and urine container. Ship refrigerated Immunoassay HOUR URINE Reportafter 2–3 or frozen. Avoid direct sunlight, antihistamines, oral weeks corticosteroids and H2 receptor blockers, 24 hours prior to specimen collection. HISTO / CYTOPATHOLO GY / Submit stained Histopathology sections/ Cytology 2 Magnifications Sample Daily by 6 IMMUNOHISTO smears / IHC stained sections. Ship at room provided on J113 Digital Photography pm; Report Next CHEMISTRY temperature in a slide mailer. Brief clinical history in Photographic paper day (IHC), Test Requisition Form is mandatory. or CD ROM. PHOTOMICROG RAPH Slides / Blocks shall be issued Only representative HISTO/CYTOPAT from the slides / blocks will HOLOGY, Submit written request for issue of slide(s) / block(s) Histopathology J119 NA be issued on advise SLIDE(S) / to department of Histo / Cytopathology. department 72 of the referring BLOCK(S), ISSUE hours after consultant. receiving the request. Also see LUPUS ERYTHEMATOSUS Sample by Thu 9 HISTONE 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S019 PANEL & am; Report Same ANTIBODIES refrigerated or frozen. Overnight fasting is preferred. Immunoassay EXTRACTABLE day NUCLEAR ANTIGENS.

PAGE 201 OF 399 HISTOPATHOLO GY, DIRECT IMMUNOFLUOR Submit Skin / Conjunctival Biopsy specimen in Cryoprocessing, Use test code J113 Sample Daily by 6 * C3 *C1q *IgA Z158 ESCENCE buffered normal saline. Biopsy should measure Fluorescence for pm; Report 4 *IgG *IgM (DIF),SKIN / minimum 3 mm. Ship refrigerated. Microscopy Photomicrograph. days CONJUNCTIVAL BIOPSY, HISTOPATHOLO GY, DIRECT IMMUNO- FLUORESCENCE Submit Skin / Conjunctival Biopsy specimen in Cryoprocessing, . Use test code J113 Sample Daily by 6 J040 (DIF),SKIN / buffered normal saline. Biopsy should measure Fluorescence for pm; Report 4 CONJUNCTIVAL minimum 3 mm.Ship refrigerated. Microscopy Photomicrograph. days BIOPSY, COMPLEMENT C1q *Complement C3 HISTOPATHOLO Submit Kidney Biopsy specimen in buffered normal *Complement GY, DIRECT saline. Kidney biopsy should measure minimum 1 Cryoprocessing, Use test code J113 Sample Daily by 6 C1q *IgA * IgG Z829 IMMUNOFLUOR cm in length. Ship refrigerated. Brief clinical history Fluorescence for pm; Report 3 *IgM *Kappa ESCENCE (DIF), in Renal Biopsy form for Native / Transplant kidneys Microscopy Photomicrograph. days Light Chains KIDNEY BIOPSY is mandatory. *Lambda Light Chains HISTOPATHOLO GY, DIRECT IMMUNO- FLUORESCENCE Submit Skin / Conjunctival Biopsy specimen in Cryoprocessing, Use test code J113 Sample Daily by 6 J024 (DIF),SKIN / buffered normal saline. Biopsy should measure Fluorescence for pm; Report 4 CONJUNCTIVAL minimum 3 mm. Ship refrigerated. Microscopy Photomicrograph. days BIOPSY, COMPLEMENT C3

PAGE 202 OF 399 HISTOPATHOLO GY, DIRECT IMMUNO- Submit Skin / Conjunctival Biopsy specimen in Cryoprocessing, Use test code J113 Sample Daily by 6 J026 FLUORESCENCE buffered normal saline. Biopsy should measure Fluorescence for pm; Report 4 (DIF),SKIN / minimum 3 mm. Ship refrigerated. Microscopy Photomicrograph. days CONJUNCTIVAL BIOPSY, IgG

HISTOPATHOLO GY, DIRECT IMMUNO- Submit Skin / Conjunctival Biopsy specimen in Cryoprocessing, Use test code J113 Sample Daily by 6 J027 FLUORESCENCE buffered normal saline. Biopsy should measure Fluorescence for pm; Report 4 (DIF),SKIN / minimum 3 mm. Ship refrigerated. Microscopy Photomicrograph. days CONJUNCTIVAL BIOPSY, IgM

Histopathology sections or paraffin embedded HISTOPATHOLO Special stains tissue blocks previously reported upon. Ship at Sample Daily by 6 GY, BIOPSY - performed only on J007 room temperature. Previous report(s), clinical Microscopy pm; Report 3 SECOND request, as history and other details to be submitted along with days OPINION additional tests. the test request form.

Use Test Code J116 for Large Bone Submit specimen in 10% Formal-saline. Formal- Resections. Bone saline should cover the specimen completely. Ship specimens require HISTOPATHOLO Sample Daily by 6 at room temperature. Brief clinical history in Processing, Staining decalciflcation & J118 GY, BIOPSY, pm; Report Histopathology Requisition Form is mandatory. For & Microscopy reporting time can BONE maximum 12 days patients with multiple specimens kindly register all vary. Special stains specimens under single Lab number. performed only on request, as additional tests.

PAGE 203 OF 399 *Esophagectomy *Gastrectomy *Mastectomy *Hemi / Total Colectomy *Large Bone Resection*Ovaria Bone specimens n Tumor HISTOPATHOLO Submit specimen in 10% Formal-saline. Formal- require Resection Sample Daily by 6 GY, BIOPSY, saline should cover the specimen completely. Ship decalciflcation and *Radical pm; Report 5 LARGE at room temperature. Brief clinical history in Processing, Staining reporting will take J116 Nephrectomy days. Bone COMPLEX / Histopathology Requisition Form is mandatory. For & Microscopy 14-16 days. Special for Cancer specimens 14 - CANCER patients with multiple specimens kindly register all stains performed *Radical Neck 16 days SPECIMEN specimens under single Lab number. only on request, as Dissection additional tests. *Radical Hysterectomy *Radical Orchidectomy *Soft tissue tumor resections *Head & Neck Ç

PAGE 204 OF 399 *Endometrium *Cervical biopsy *Endoscopic Submit specimen in 10% Formal-saline. Formal- biopsies *Trucut Sample Daily by HISTOPATHOLO saline should cover the specimen completely. Ship Special stains biopsies 11 am; Report GY, BIOPSY, at room temperature. Brief clinical history in Processing, Staining performed only on J001 *Appendix Nextday Sample SMALL Histopathology Requisition Form is mandatory. For & Microscopy request, as *FallopianTubes Daily 11 am to 6 SPECIMEN patients with multiple specimens kindly register all additional tests. *Conjunctival pm; Report 2days specimens under single Lab number. biopsy *Small diagnostic / Incision biopsies

*Histopathology *Special Stains Submit 2 Kidney Biopsy specimens; 1 specimen in Each Biopsy should *DIF for IgG, IgA, Cryoprocessing, buffered normal saline for DIF studies AND 1 measure minimum 1 HISTOPATHOLO IgM, C3 & C1q Fluorescence and Sample Daily by 6 specimen in 10% formal saline for Histopathology. cm in length. Special Z353 GY, KIDNEY *IHC for C4d & Light Microscopy, pm; Report 5 Ship refrigerated. Brief clinical history in Renal stains include PAS, BIOPSY PANEL 1 SV40 (Transplant IHC, Digital days Biopsy form for Native / Transplant kidneys is Masson Trichrome, biopsy) Photography mandatory. Silver & Congo Red. *Photomicrograp h *Histopathology *Special Stains Each Biopsy should *IHC for C4d & Submit Kidney Biopsy specimen in 10% formal measure minimum 1 HISTOPATHOLO Light Microscopy, Sample Daily by 6 SV40 saline. Ship at room temperature. Brief clinical cm in length. Special Z355 GY, KIDNEY IHC, pm; Report 5 (Transplant history in Renal Biopsy form for Native / Transplant stains include PAS, BIOPSY PANEL 2 DigitalPhotography days biopsy) kidneys is mandatory. Masson Trichrome, *Photomicrograp Silver & Congo Red. h

PAGE 205 OF 399 *Histopathology *Special Stains Submit 3 Kidney Biopsy specimens; 1 specimen in Cryoprocessing, *DIF for IgG,IgA, Each Biopsy should buffered normal saline for DIF studies AND 1 Fluorescence and IgM, C3 & C1q measure minimum 1 HISTOPATHOLO specimen in 10% formal saline for Histopathology Light Microscopy, Sample Daily by 6 *IHC for C4d & cm in length. Special Z830 GY, KIDNEY AND 1 specimen in 3% Bufferred Glutaraldehyde IHC, Digital pm; Report 7 SV40 (Transplant stains include PAS, BIOPSY PANEL 3 solution available from LPL. Ship refrigerated. Brief Photography, days biopsy) Masson Trichrome, clinical history in Renal Biopsy form for Native / Transmission *Photomicrograp Silver & Congo Red. Transplant kidneys is mandatory. Electron Microscopy h * Electron Microscopy

HISTOPATHOLO Histopathology sections or paraffin embedded Special Stains GY, KIDNEY tissue blocks previously reported upon. Ship at Performed On Sample Daily by 6 Z850 BIOPSY room temperature. Previous report(s), clinical Light Microscopy Request Depending pm; Report 4 SECOND history and other details to be submitted along with On Availability Of days OPINION the test request form. Unstained Slides.

HISTOPATHOLO GY, DIRECT IMMUNOFLUOR Submit Skin / Conjunctival Biopsy specimen in Cryoprocessing, Use test code J113 Sample Daily by 6 J025 ESCENCE (DIF), buffered normal saline. Biopsy should measure Fluorescence for pm; Report 4 SKIN / minimum 3 mm. Ship refrigerated. Microscopy Photomicrograph. days CONJUNCTIVAL BIOPSY, IgA

PAGE 206 OF 399 *Breast lump *Pilonidal sinus *Fistula / Sinus *Lymph node *Ovarian cyst *Gall bladder Submit specimen in 10% Formal-saline. Formal- *Prostate (TURP HISTOPATHOLO saline should cover the specimen completely. Ship Special stains / Enucleation) Sample Daily by 6 GY, BIOPSY, at room temperature. Brief clinical history in Processing, Staining performed only on J092 *Superficial pm; Report 3 MEDIUM Histopathology Requisition Form is mandatory. For & Microscopy request, as lumps *Brain & days SPECIMEN patients with multiple specimens kindly register all additional tests. Spinal cord specimens under single Lab number. tumors * Small excision biopsies *Fibroids (Enucleated) *Products of conception

Biopsy should measure minimum 1.5 cm in length. *Histopathology HISTOPATHOLO Submit Liver Biopsy specimen in 10% formal saline. Special stains Sample Daily by 6 *Special Stains Light Microscopy, Z386 GY, LIVER Ship at room temperature. Brief clinical history in include PAS, PAS-D, pm; Report 5 *Photomicrograp Digital Photography BIOPSY PANEL Histopathology Requisition Form ismandatory. Masson Trichrome, days h Iron & Reticulin. Congo Red Staining is done if required.

PAGE 207 OF 399 HISTOPATHOLO Submit Histopathology Sections OR Paraffin Sample Daily by 6 GY, embedded tissue blocks. Ship at room temperature. Polarising J114 pm; Report Next POLARISINGMIC Previous report(s), clinical history and other details Microscopy day ROSCOPY to be submitted along with the test request form.

Submit tissue biopsy in 10% formal saline OR HISTOPATHOLO formalin fixed paraffin embedded tissue block OR GY, SPECIAL Sample Daily by 6 FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J050 STAIN, ACID pm; Report 3 slides and air dried. Ship at room temperature. Brief Microscopy FAST (Z.N days clinical history in Histopathology Requisition Form is STAIN) mandatory.

Submit tissue biopsy in 10% formal saline OR HISTOPATHOLO formalin fixed paraffin embedded tissue block OR Sample Daily by 6 GY, SPECIAL FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J084 pm; Report 3 STAIN, ALCIAN slides and air dried. Ship at room temperature. Brief Microscopy days BLUE clinical history in Histopathology Requisition Form is mandatory.

Submit tissue biopsy in 10% formal saline OR HISTOPATHOLO formalin fixed paraffin embedded tissue block OR GY, SPECIAL Sample Daily by 6 FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J052 STAIN, pm; Report 3 slides and air dried. Ship at room temperature. Brief Microscopy AMYLOID days clinical history in Histopathology Requisition Form is (CONGO RED) mandatory.

Submit tissue biopsy in 10% formal saline OR HISTOPATHOLO formalin fixed paraffin embedded tissue block OR Sample Daily by 6 GY, SPECIAL FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J095 pm; Report 3 STAIN, COPPER slides and air dried. Ship at room temperature. Brief Microscopy days (RHODANINE) clinical history in Histopathology Requisition Form is mandatory.

PAGE 208 OF 399 Submit tissue biopsy in 10% formal saline OR HISTOPATHOLO formalin fixed paraffin embedded tissue block OR GY, SPECIAL Sample Daily by 6 FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J078 STAIN, pm; Report 3 slides and air dried. Ship at room temperature. Brief Microscopy FONTANA days clinical history in Histopathology Requisition Form is MASSON mandatory.

HISTOPATHOLO Submit tissue biopsy in 10% formal saline OR GY, SPECIAL formalin fixed paraffin embedded tissue block OR Sample Daily by 6 STAIN, FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J079 pm; Report 3 GOMORI’S slides and air dried. Ship at room temperature. Brief Microscopy days METHENAMINE clinical history in Histopathology Requisition Form is SILVER mandatory.

Submit tissue biopsy in 10% formal saline OR HISTOPATHOLO formalin fixed paraffin embedded tissue block OR Sample Daily by 6 GY, SPECIAL FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J053 pm; Report 3 STAIN, IRON slides and air dried. Ship at room temperature. Brief Microscopy days (PERL’S STAIN) clinical history in Histopathology Requisition Form is mandatory.

Submit tissue biopsy in 10% formal saline OR HISTOPATHOLO formalin fixed paraffin embedded tissue block OR GY, SPECIAL Sample Daily by 6 FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J081 STAIN, pm; Report 3 slides and air dried. Ship at room temperature. Brief Microscopy MASSON days clinical history in Histopathology Requisition Form is TRICHROME mandatory.

PAGE 209 OF 399 Submit tissue biopsy in 10% formal saline OR HISTOPATHOLO formalin fixed paraffin embedded tissue block OR Sample Daily by 6 GY, SPECIAL FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J080 pm; Report 3 STAIN, slides and air dried. Ship at room temperature. Brief Microscopy days MUCICARMINE clinical history in Histopathology Requisition Form is mandatory.

Submit tissue biopsy in 10% formal saline OR HISTOPATHOLO formalin fixed paraffin embedded tissue block OR GY, SPECIAL Sample Daily by 6 FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J054 STAIN, pm; Report 3 slides and air dried. Ship at room temperature. Brief Microscopy PERIODIC ACID days clinical history in Histopathology Requisition Form is SCHIFF (PAS) mandatory. HISTOPATHOLO Submit tissue biopsy in 10% formal saline OR GY, SPECIAL formalin fixed paraffin embedded tissue block OR STAIN, Sample Daily by 6 FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J082 PHOSPHOTUNG pm; Report 3 slides and air dried. Ship at room temperature. Brief Microscopy STIC ACID days clinical history in Histopathology Requisition Form is HEMATOXYLIN mandatory. (PTAH) Submit tissue biopsy in 10% formal saline OR HISTOPATHOLO formalin fixed paraffin embedded tissue block OR Sample Daily by 6 GY, SPECIAL FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J059 pm; Report 3 STAIN, slides and air dried. Ship at room temperature. Brief Microscopy days RETICULIN clinical history in Histopathology Requisition Form is mandatory.

PAGE 210 OF 399 Submit tissue biopsy in 10% formal saline OR HISTOPATHOLO formalin fixed paraffin embedded tissue block OR Sample Daily by 6 GY, SPECIAL FNAC specimen, alcohol fixed, smeared onto 4 glass Special stain, J083 pm; Report 3 STAIN, VAN slides and air dried. Ship at room temperature. Brief Microscopy days GIESONS clinical history in Histopathology Requisition Form is mandatory.

Submit specimen in 10% Formal-saline. Formal- Histopathology saline should cover the specimen completely. Ship HISTOPATHOLO examination Sample Daily by 6 at room temperature. Brief clinical history in Processing, Staining Z701 GY,SKIN BIOPSY *Special stains pm; Report 5 Histopathology Requisition Form is mandatory. For & Microscopy PANEL *Photomicrograp days patients with multiple specimens kindly register all h specimens under single Lab number. HISTOPLASMA Sample by 7th of ANTIBODY, 1 mL (0.5 mL min.) Serum from 1 SST. Ship the month; RW269 Immunodiffusion IMMUNODIFFU refrigerated or frozen. Reportafter 2–3 SION weeks HIV 1 & 2 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent ANTIBODIES, Includes p24 S010 refrigerated or frozen. Patient consent on HIV MicroparticleImmun Daily SCREENING Antigen Consent form is mandatory. oassay TEST Sample by Tue / HIV 1 & 2 2 mL (1 mL min.) serum from 1 SST. Ship Thu / Sat 9 S063 ANTIBODIES, Western Blot refrigerated or frozen. am;Report Same WESTERN BLOT day 4 mL (2 mL min.) plasma from 1 Lavender Top HIV-2 (EDTA) tube. Separate plasma aseptically within 2 Sample by Mon OS151 DETECTION , PCR hours of collection. Ship refrigerated or frozen. 12 11 am; Report Fri PCR hours fasting is recommended.

PAGE 211 OF 399 HIV 1 2 mL (1 mL min.) plasma from 1 Lavender Top Sample by 1st / Reverse GENOTYPING & (EDTA) tube. Separate plasma within 6 hours of 15th; Report on N060 Transcriptase PCR DRUG collection in a plastic transport vial and freeze 15th / 30th of and DNA Sequencing RESISTANCE immediately. Ship frozen. DO NOT THAW. every month;

HIV 1 RNA PCR: 3 mL (2 mL min.) plasma from 2 Lavender Top QUANTITATIVE, (EDTA) tubes. Separate plasma aseptically within 2 REAL TIME PCR hours ofcollection. Ship refrigerated or frozen. 12 WITH CD3, CD4 Sample by Wed / hours fasting is recommended. FLOW: 4 mL (3 mL Real Time PCR, Flow H216 / CD8 Sat 11 am; min.) whole blood from 1 Lavender Top (EDTA) AND Cytometry (IMMUNE ReportFri / Tue 1 Green top (Sodium Heparin) tube each. Ship at 18- DEFICIENCY 22°C. DO NOT REFRIGERATE OR FREEZE. Specify PANEL 2) time, date and clinical details on Test Request Form. MARKERS 3 mL (2 mL min.) plasma from 2 Lavender Top HIV 1 RNA Sample by Wed / (EDTA) tubes. Separate plasma aseptically within 2 Avoid freeze thaw N029 QUANTITATIVE, Real Time PCR Sat 11 am; hours of collection. Ship refrigerated or frozen. 12 cycles. REAL TIME PCR Report Fri/ Tue hours fasting is recommended.

HIV 1 RNA PCR: 3 mL (2 mL min.) plasma from 2 Lavender Top QUANTITATIVE, (EDTA) tubes. Separate plasma aseptically within 2 REAL TIME PCR hours of collection. Ship refrigerated or frozen. 12 Sample by Wed / WITH CD3 / hours fasting is recommended.FLOW: 4 mL (3 mL Real Time PCR, Flow H215 Sat 11 am; CD4 (IMMUNE min.) whole blood from 1 Lavender Top (EDTA) AND Cytometry ReportFri / Tue DEFICIENCY 1 Green top (Sodium Heparin) tube each. Ship at 18- PANEL 4) 22°C. DO NOT REFRIGERATE OR FREEZE. Specify MARKERS time, date and clinical details on Test Request Form.

4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L021 HLA - A2 (A*02) a.m; Report Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE.

PAGE 212 OF 399 Doctor's prescription on the 4 mL (3 mL min.) Whole blood in 1 Lavender Top HLA - ABC letter head with Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L025 (CLASS 1) stamp AND 1 a.m; Report Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex TYPING photograph of Nextday FREEZE. recipient / donor is mandatory. Doctor's prescription on the 4 mL (3 mL min.) Whole blood in 1 Lavender Top HLA - AB letter head with Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L005 (CLASS 1) stamp AND 1 a.m; Report Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex TYPING photograph of Nextday FREEZE. recipient / donor is mandatory. 4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 HLA - B22 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L019 a.m; Report (B*22) Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE. 4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L026 HLA - B7 (B*07) a.m; Report Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE. 4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 HLA - B38 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L009 a.m; Report (B*38) Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE. 4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 HLA - B5 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L008 a.m; Report (B*51/52) Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE. 4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L007 HLA - B8 (B*08) a.m; Report Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE.

PAGE 213 OF 399 4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 HLA - B15 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L010 a.m; Report (B*15) Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE. 4 mL (2 mL min.) whole blood in 1 Lavender Top Sample Daily by 9 HLA-B27, FLOW L004 (EDTA) / Green Top (Sodium Heparin) tube. Ship at Flow Cytometry am; Report Same CYTOMETRY 18–22°C. DO NOT REFRIGERATE OR FREEZE. Day 4 mL (3 mL min.) Whole blood in 1 Lavender Top HLA B LOCUS Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L028 TYPING - ANY a.m; Report Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex ANTIGEN Nextday FREEZE. HLA - B27, Sample Daily by 9 3 mL (2 mL min.) whole blood from 1 Lavender Top N044 QUALITATIVE Real Time PCR am; Report Same (EDTA) tube. Ship refrigerated. DO NOT FREEZE. PCR Day

Doctor's prescription on the letter head with stamp AND RECIPIENT: 6 mL (3 mL min.) whole blood in 1 photograph of Yellow Top (ACD) tube AND 3 mL (2 mL min.) serum recipient is from 1 SST. Collect specimen preferably 48 hours Complement mandatory. Above HLA after dialysis.CADAVERIC DONOR: 12 mL (10 mL Dependent specimen Sample Daily; L038 CADAVERIC min.) whole blood in 2 Yellow Top (ACD) tubes. Ship Cytotoxicity with requirements are for Report 8 hrs CROSS MATCH refrigerated. DO NOT FREEZE. Brief history in Organ DTT treatment a single donor being Transplant Clinical information & Consent form is cross matched with mandatory. a single receipient. For additional cross matches, send additional specimens of donÇ

PAGE 214 OF 399 HLA - CELIAC DISEASE (DR- 4 mL (3 mL min.) Whole blood in 1 Lavender Top DQB1-DQA1 For Pediatric cases Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 L017 HAPLOTYPE SSP - PCR use Microtainers a.m; Report Yellow Top (ACD) tube. Ship refrigerated. DO NOT ASSOCIATION available from LPL Nextday FREEZE. INCLUDING DQ2,DQ8) 4 mL (3 mL min.) Whole blood in 1 Lavender Top HLA C LOCUS Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L032 TYPING, ANY a.m; Report Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex ANTIGEN Nextday FREEZE. 4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 HLA - Cw6 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L020 a.m; Report (C*06) Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE. HLA DNA TYPING FOR *A, B, C, DR & 4 mL (3 mL min.) Whole blood in 1 Lavender Top Also see OPPOSITE BONE Sample Daily by 9 DQ Low (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 SEX BMT MISMATCH N024 MARROW SSP - PCR a.m; Report 2 Resolution Yellow Top (ACD) tube. Ship refrigerated. DO NOT AND CHIMERISM TRANSPLANT, days Typing FREEZE. tests. 10 ANTIGENS, PCR 4 mL (3 mL min.) Whole blood in 1 Lavender Top HLA DNA HIGH (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Sample Daily by 9 RESOLUTION N086 Yellow Top (ACD) tube OR Buccal swab collected in PCR, Sequencing a.m; Report 7 TYPING - A a sterile container. Ship refrigerated. DO NOT days. LOCUS FREEZE. 4 mL (3 mL min.) Whole blood in 1 Lavender Top HLA DNA HIGH (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Sample Daily by 9 RESOLUTION N070 Yellow Top (ACD) tube OR Buccal swab collected in PCR, Sequencing a.m; Report 8 TYPINGA, B, C, a sterile container. Ship refrigerated. DO NOT days. DR & DQ FREEZE.

PAGE 215 OF 399 Doctor's HLA DNA prescription on the 4 mL (3 mL min.) Whole blood in 1 Lavender Top TYPING FOR *A, B & DR Low letter head with Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - N023 KIDNEY / LIVER Resolution stamp AND 1 a.m; Report 2 Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex TRANSPLANT, Typing photograph each of days FREEZE. PCR recipient and donor is mandatory. 4 mL (3 mL min.) Whole blood in 1 Lavender Top HLA DNA HIGH (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Sample Daily by 9 RESOLUTION N087 Yellow Top (ACD) tube OR Buccal swab collected in PCR, Sequencing a.m; Report 7 TYPING - B a sterile container. Ship refrigerated. DO NOT days. LOCUS FREEZE. 4 mL (3 mL min.) Whole blood in 1 Lavender Top HLA DNA HIGH (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Sample Daily by 9 RESOLUTION N083 Yellow Top (ACD) tube OR Buccal swab collected in PCR, Sequencing a.m; Report 7 TYPING - C a sterile container. Ship refrigerated. DO NOT days. LOCUS FREEZE. 4 mL (3 mL min.) Whole blood in 1 Lavender Top HLA DNA HIGH (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Sample Daily by 9 RESOLUTION N085 Yellow Top (ACD) tube OR Buccal swab collected in PCR, Sequencing a.m; Report 7 TYPING - DQB1 a sterile container. Ship refrigerated. DO NOT days. LOCUS FREEZE. 4 mL (3 mL min.) Whole blood in 1 Lavender Top HLA DNA HIGH (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Sample Daily by 9 RESOLUTION N084 Yellow Top (ACD) tube OR Buccal swab collected in PCR, Sequencing a.m; Report 7 TYPING - DRB1 a sterile container. Ship refrigerated. DO NOT days. LOCUS FREEZE.

PAGE 216 OF 399 HLA DNA TYPING FOR 4 mL (3 mL min.) Whole blood in 1 Lavender Top BONE *A, B & DR Low Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - N025 MARROW Resolution a.m; Report 2 Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex TRANSPLANT, 6 Typing days FREEZE. ANTIGENS, LUMINEX HLA DONOR SPECIFIC IgG RECIPIENT: 3 mL (2 mL min.) serum from 1 SST. ANTIBODIES & Collect specimen preferably 48 hours after dialysis. Sample by Tue / Luminex Fluoro - PANEL DONOR: 12 mL (10 mL min.) whole blood in 2 Thu / Sat 9 L037 Beads X-Map REACTIVE Yellow Top (ACD) tubes. Ship refrigerated. DO NOT amReport Wed / technology ANTIBODIES FREEZE. Brief history in Organ Transplant Clinical Fri / Mon 6 pm SCREEN information & Consent form form is mandatory. PROFILE Doctor's prescription on the 4 mL (3 mL min.) Whole blood in 1 Lavender Top letter head with Sample Daily by 9 HLA - DQB1 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L036 stamp AND 1 a.m; Report TYPING Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex photograph of Nextday FREEZE. recipient / donor is mandatory. 4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 HLA - DR7 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L016 a.m; Report (DRB1*07) Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE. Doctor's prescription on the 4 mL (3 mL min.) Whole blood in 1 Lavender Top HLA - DR & letter head with Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L018 DQB1 (CLASS II) stamp AND 1 a.m; Report Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex TYPING photograph of Nextday FREEZE. recipient / donor is mandatory.

PAGE 217 OF 399 Doctor's prescription on the 4 mL (3 mL min.) Whole blood in 1 Lavender Top HLA - DR letter head with Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L006 (CLASS II) stamp AND 1 a.m; Report Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex TYPING photograph of Nextday FREEZE. recipient / donor is mandatory. 4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 HLA - DR5 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L014 a.m; Report (DRB1*11/12) Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE. 4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 HLA - DR3 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L012 a.m; Report (DRB1*03) Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE. 4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 HLA - DR4 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L013 a.m; Report (DRB1*04) Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE. 4 mL (3 mL min.) Whole blood in 1 Lavender Top Sample Daily by 9 HLA - DR2 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Reverse SSOP - L011 a.m; Report (DRB1*02) Yellow Top (ACD) tube. Ship refrigerated. DO NOT Luminex Nextday FREEZE.

PAGE 218 OF 399 Doctor's prescription on the letter head with stamp AND 1 RECIPIENT: 3 mL (2 mL min.) serum from 1 photograph each of HLA DSA SST.Collect specimen preferably 48 hours after recipient and donor Sample by Tue (DONOR dialysis.DONOR: 12 mL (10 mL min.) whole blood in is mandatory. Above Luminex X-Map /Thu / Sat 9 L022 SPECIFIC IgG 2 Yellow Top (ACD) tubes. Ship refrigerated. DO specimen technology am;Report Wed / ANTIBODIES), NOT FREEZE. Brief history in Organ Transplant requirements are for Fri / Mon CLASS I & II Clinical information & Consent form form is a single donor being mandatory. cross matched with a single receipient. For additional cross matches, send additional sÇ

HLA 4 mL (3 mL min.) Whole blood in 1 Lavender Top HYPERSENSITIVI (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Sample Daily by 9 L029 TY TO Yellow Top (ACD) tube OR Buccal swab collected in PCR, Sequencing a.m; Report 7 ABACAVIR a sterile container. Ship refrigerated. DO NOT days. (B*57;01) FREEZE. HLA 4 mL (3 mL min.) Whole blood in 1 Lavender Top HYPERSENSITIVI (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Sample Daily by 9 TY TO L030 Yellow Top (ACD) tube OR Buccal swab collected in PCR, Sequencing a.m; Report 7 CARBAMAZEPIN a sterile container. Ship refrigerated. DO NOT days. E & PHENYTOIN FREEZE. (B*15;02) HLA 4 mL (3 mL min.) Whole blood in 1 Lavender Top HYPERSENSITIVI (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Sample Daily by 9 L031 TY TO Yellow Top (ACD) tube OR Buccal swab collected in PCR, Sequencing a.m; Report 7 ALLOPURINOL a sterile container. Ship refrigerated. DO NOT days. (B*58;01) FREEZE.

PAGE 219 OF 399 HLA - 4 mL (3 mL min.) Whole blood in 1 Lavender Top NARCOLEPSY Sample Daily by 9 (EDTA) tube OR 6 mL (3 mL min.) Whole blood in 1 Sequencing Based L027 (DRB1*15, a.m; Report 7 Yellow Top (ACD) tube. Ship refrigerated. DO NOT Typing (SBT) DQB1*06:02, days. FREEZE. DQA1*01:02)

HLA - PANEL 3 mL (1.5 mL min.) serum from 1 SST. Ship REACTIVE IgG refrigerated or frozen.Collect specimen preferably Luminex X-Map Sample by Thu; L024 ANTIBODIES 48 hours after dialysis. Brief history in Organ technology Report Sat (PRA), CLASS II, Transplant Clinical information & Consent form is QUANTITATIVE mandatory.

HLA - PANEL 3 mL (1.5 mL min.) serum from 1 SST. Ship REACTIVE IgG refrigerated or frozen.Collect specimen preferably Luminex X-Map Sample by Thu; L023 ANTIBODIES 48 hours after dialysis. Brief history in Organ technology Report Sat (PRA), CLASS I, Transplant Clinical information & Consent form is QUANTITATIVE mandatory.

HLA - PANEL 3 mL (1.5 mL min.) serum from 1 SST. Ship Sample by Mon / REACTIVE IgG refrigerated or frozen.Collect specimen preferably Luminex X-Map Wed / Fri 9 L003 *Class I *Class II ANTIBODIES 48 hours after dialysis. Brief history in Organ technology amReport Tue / (PRA), SCREEN Transplant Clinical information form is mandatory. Thu / Sat

HLA SINGLE ANTIGEN BEAD 2 mL (1 mL min) serum from 1 SST .Ship refrigerated Luminex X-Map Sample by Thu 9 L035 ASSAY FOR or frozen. technology am; Report Sat CLASS II IgG ANTIBODIES

PAGE 220 OF 399 HLA SINGLE ANTIGEN BEAD 2 mL (1 mL min) serum from 1 SST . Ship Luminex X-Map Sample by Thu 9 L034 ASSAY FOR refrigerated or frozen. technology am; Report Sat CLASS I IgG ANTIBODIES

Doctor's prescription on the letter head with stamp AND 1 RECIPIENT: 6 mL (3 mL min.) whole blood in 1 photograph each of Yellow Top (ACD) tube AND 3 mL (2 mL min.) serum recipient and donor from 1 SST. Collect specimen preferably 48 hours Complement HLA T & B CELL is mandatory. Above Sample Daily by 1 after dialysis.DONOR: 12 mL (10 mL min.) whole Dependent L002 LYMPHOCYTE specimen pm; Report Next blood in 2 Yellow Top (ACD) tubes. Ship Cytotoxicity with CROSS MATCH requirements are for day refrigerated. DO NOT FREEZE. Brief history in Organ DTT treatment a single donor being Transplant Clinical information & Consent form is cross matched with mandatory. a single receipient. For additional cross matches, send additional sÇ

PAGE 221 OF 399 RECIPIENT: 2 mL (1 mL min) serum from 1 SST AND 4 mL (2 mL min) whole blood from 1 Green Top (Sodium Heparin) tube. DONOR: 3 mL (2 mL min) whole blood in 3 Green Top (Sodium Heparin) tubes. Ship immediately at 18- 22 ͦ C. DO NOT FREEZE. Brief history in Organ Transplant Clinical HLA T & B CELL information & Consent form is mandatory. Doctor's Sample Daily by 9 FLOW L033 prescription on the letter head with stamp AND 1 Flow cytometry am; Report Next CYTOMETRY, photograph each of recipient and donor is day CROSSMATCH mandatory. Above specimen requirements are for a single donor being cross matched with a single receipient. For additional cross matches, send additional specimens of donors. Request as additional tests. Collect specimen preferably 48 hours after dialysis.

PAGE 222 OF 399 *Uterus with cervix *Uterus with cervix and adnexae / fibroids *Ovarian tumors / Large cysts *Eyeball Submit specimen in 10% Formal-saline. Formal- HlSTOPATHOLO (tumorous) saline should cover the specimen completely. Ship Special stains Sample Daily by 6 GY, BIOPSY, *Non tumorous at room temperature. Brief clinical history in Processing, Staining performed only on J012 pm; Report 4 LARGE conditions of Histopathology Requisition Form is mandatory. For & Microscopy request, as days SPECIMEN Thyroid gland / patients with multiple specimens kindly register all additional tests. Testes / Kidney - specimens under single Lab number. nephrectomy / Intestinal resection *Lymph node (Block dissection) *Placenta *EyeÇ

Submit Nasal / Throat / Nasopharyngeal Swab H1N1 (SWINE Sample Daily by inoculated in special viral transport medium FLU) 11 am: Report N034 available from LPL. Ship refrigerated in a special Real Time PCR QUALITATIVE, Same Day transport box available from LPL. Clinical History is REAL TIME PCR (except Sundays) mandatory

PAGE 223 OF 399 * Glucose, HOMA-IR; Fasting *C- 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium INSULIN Peptide, Fasting Sample Daily by fluoride) tube AND 2 mL (1 mL min.) serum from 1 Z828 RESISTANCE *Beta cell Hexokinase, CLIA 4 pm; Report SST. Ship refrigerated or frozen. Overnight fasting is INDEX, C- function *Insulin Same Day mandatory. PEPTIDE MODEL sensitivity *HOMA-IR index

*Glucose-F * HOMA-IR ; Insulin-F*Beta 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium Hexokinase, Sample Daily by INSULIN cell function* fluoride) tube AND 2 mL (1 mL min.) serum from 1 Chemiluminescent Z827 4 pm; Report RESISTANCE Insulin SST. Ship refrigerated or frozen. Overnight fasting is MicroparticleImmun Same Day INDEX sensitivity*HOM mandatory. oassay A-IR index HOMOCYSTEINE 2 mL (1 mL min.) serum from 1 SST. Place the Chemiluminescent , sample immediately on ice & separate serum within R143 MicroparticleImmun Daily QUANTITATIVE, 1 hour of collection. Ship refrigerated or frozen. oassay SERUM Overnight fasting is preferred. 50 mL (10 mL min.) aliquot of 24 Hour urine. Add HOMOVANILLIC 50% HCl drop by drop to bring the pH between 1–2. High Performance Also see NEURONE Sample by Wed / U036 ACID (HVA), 24- Do not use concentrated HCl. Ship refrigerated or LiquidChromatograp SPECIFIC ENOLASE Sat 9 am; Report HOUR URINE frozen. Patient should strictly avoid L-Dopa at least hy and VMA. Fri / Tue 72 hours before and during collection.

50 mL (10 mL min.) aliquot of random urine. Add HOMOVANILLIC 50% HCl drop by drop to bring the pH between 1–2. High Performance Also see NEURONE Sample by Wed / ACID (HVA), U049 Do not use concentrated HCl. Ship refrigerated or LiquidChromatograp SPECIFIC ENOLASE Sat 9 am; Report RANDOM frozen. Patient should strictly avoid L-Dopa at least hy and VMA. onFri / Tue URINE 72 hours before and during collection.

PAGE 224 OF 399 HPV, CHLAMYDIA TRACHOMATIS Sample by Wed / Submit cervical swab in a special container available Z794 & NEISSERIA Real Time PCR Sat 11 am. from LPL. Ship refrigerated. DO NOT FREEZE GONORRHEA Report Fri /Tue PCR PANEL, QUALITATIVE HPV (HUMAN PAPILLOMA Submit Cervical swab OR Oral swab in a special VIRUS) Sample by Wed / container available from LPL OR Paraffin embedded N040 DETECTION & Real Time PCR Sat 11 am; tissue block OR Fresh tissue in normal saline. Ship GENOTYPING, Report Fri / Tue refrigerated. DO NOT FREEZE QUALITATIVE, PCR Sample by 7th of HTLV - I / II 2 mL (1 mL min.) serum from 1 SST. Ship the month; RW097 ANTIBODY refrigerated or frozen in plastic screw capped vial. Line Immunoassay Reportafter 2–3 CONFIRMATION DO NOT USE GLASS TUBES. weeks. Sample by 7th of HTLV - I / II 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescence, the month; RW096 ANTIBODY, refrigerated or frozen in plastic screw capped vial. Immunoassay Reportafter 2–3 SCREEN DO NOT USE GLASS TUBES. weeks HUMAN HERPESVIRUS 6 3 ml (2 mL min.) Whole blood from 1 Lavender Top Sample by N130 (HHV6), (EDTA) tube OR 3 ml (2 mL min.) CSF. Ship Real Time PCR Mon/Thu 11 am; QUALITATIVE refrigerated. DO NOT FREEZE. Report Wed/Sat PCR HUMAN 3 ml (2 mL min.) Whole blood from 1 Lavender Top Sample by PARECHOVIRUS N129 (EDTA) tube OR 3 ml (2 mL min.) CSF. Ship Real Time PCR Mon/Thu 11 am; QUALITATIVE, refrigerated. DO NOT FREEZE. Report Wed/Sat PCR

PAGE 225 OF 399 HUMAN HERPES VIRUS 3 ml (2 mL min.) Whole blood from 1 Lavender Top Sample by N123 7 (HHV7), (EDTA) tube OR 3 ml (2 mL min.) CSF. Ship Real Time PCR Mon/Thu 11 am; QUALITATIVE refrigerated. DO NOT FREEZE. Report Wed/Sat PCR HUNTINGTON Sample by Tue 11 DISEASE 4 mL (2 mL min.) Whole Blood in 1 Lavender top PCR Fragment N118 am; Report 10 MUTATION (EDTA) tube. Ship refrigerated. DO NOT FREEZE. analysis days DETECTION 17 - HYDROXYPROG 1 drop of heel prick blood each on 3 spots of filter Sample by Tue / ESTERONE, paper available from LPL. Clinical details and drug R163 Fluoroimmunoassay Thu / Sat 5 pm; NEWBORN history must accompany sample. Ship refrigerated Report 2 days SCREEN; CAH or frozen SCREEN 20 mL (10 mL min.) well mixed aliquot of 24-hour 17 - HYDROXY- urine. Add appropriate volume of 50% HCl to Sample Daily by 4 CORTICOSTEROI Column U032 maintain pH below 5. Record 24-hour urine volume pm; Report 3 DS (17-OHCS), Chromatography on test request form and urine container. Ship days 24-HOUR URINE refrigerated or frozen.

17 - HYDROXY- 20 mL (10 mL min.) well mixed aliquot of 24-hour CORTICOSTEROI urine. Add appropriate volume of 50% HCl to Sample Daily by 4 Column Z086 DS &17 - maintain pH below 5. Record 24 hour urine volume pm; Report 3 Chromatography KETOSTEROIDS, on test request form and urine container. Ship days 24-HOUR URINE refrigerated or frozen.

Sample by 7th of 17 - 2 mL (1 mL min.) serum from 1 Red Top (No the month; RW100 HYDROXYPREG LC-MS/MS Additive) tube. Ship refrigerated or frozen Reportafter 2–3 NENOLONE weeks

PAGE 226 OF 399 17 - Also see 17- 2 mL (1 mL min.) serum from 1 SST. Ship Sample Tue / Sat HYDROXYPROG HYDROXY- R053 refrigerated or frozen. An early morning specimen is Radioimmunoassay by 9 am; Report ESTERONE (17- PROGESTERONESTIM preferred. Same Day OHP) ULATION BY ACTH.

2 mL (0.5 mL min.) serum from 1 SST for each timed Synthetic ACTH 17- specimen. Draw fasting early morning baseline (Synacthen) is HYDROXYPROG sample; administer 250 ug of synthetic ACTH notsupplied by Sample Tue / Sat ESTERONE Z024 (Synacthen) IV, draw additional sample at 60 min. Radioimmunoassay LPL.Refer to Y Test by 9 am; Report (17–OHP) Ship refrigerated or frozen. Both tubes must be codes :Y106 Basal Same Day STIMULATION clearly marked with time drawn. Submit both tubes Y108 60 min. Y109 BY ACTH with 1 test request form. Customized

2 mL (1 mL min.) plasma from 1 Green Top (Sodium Non fasting samples Sample by Mon / HYDROXYPROLI Heparin) tube. Separate plasma into sterile screw G186 LC/MS acceptable Wed 5 pm; NE, PLASMA capped vial. Ship refrigerated or frozen. Overnight forpediatric patients Report 3 days fasting is mandatory.

*PTH (Intact) 6 mL (4 mL min.) serum from 2 SST's collected *Calcium, Serum between 8-10 a.m. Avoid use of tourniquet and fist HYPERCALCEMI CMIA, Arsenazo III, Also see PTH - Z165 *Phosphorus,Ser clenching during sampling. Separate serum and Daily A PANEL 2 CLIA, Molybdate UV RELATED PEPTIDE. um * Vitamin D freeze immediately. Ship frozen. DO NOT THAW. 25 Hydroxy Overnight fasting is preferred.

*Urea *Creatinine 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium *Glucose, Hexokinase, HYPERTENSION Fluoride) tube AND 2 mL (1 mL min.) serum from 1 Z311 Fasting Spectrophotometry,I Daily PANEL SST. Ship refrigerated. DO NOT FREEZE. Minimum *Electrolytes, ndirect ISE 12 hours fasting is mandatory. serum *Lipid Profile Basic

PAGE 227 OF 399 *Free T4 * TSH * HYPERTHYROID Sample Daily by 4 Total T3 *TSH 4 mL ( 3 mL min.) serum from 1 SST. Ship Z798 / GRAVE'S CLIA, ECLIA pm; Report Same Receptor refrigerated or frozen. DISEASE PANEL day Antibody 3 mL (1 mLmin.) serum from 1 SST collected between 8-10 a.m. Separate serum and freeze immediately. Avoid use of tourniquet and fist *PTH (Intact) clenching during sampling AND 10 mL (5 mL min.) HYPERCALCEMI *Calcium, Serum Z175 aliquot of 24-hour urine collected with 10 mL of CMIA, Arsenazo III Daily A PANEL 1 *Calcium 24- 50% HCl to maintain pH below 3. Overnight fasting hour Urine is preferred. Ship frozen. DO NOT THAW. Record 24 hour urine volume & body weight on test request form and urine container.

*Aspergillus fumigatus *Micropolyspora HYPERSENSITIVI Sample by 7th of faeni*Pigeon TY 2 mL (1 mL min.) serum from 1 SST. Ship the month; RW102 serum *S.viridis Immunodiffusion PNEUMONITIS refrigerated or frozen. Reportafter 2–3 *Thermoactinom SCREEN weeks yces candidus *Thermoactinom yces vulgaris

HYPOGONADIS *FSH *LH 2 mL (1 mL min) serum from 1 Red Top (No Sample Daily by 4 G201 M PANEL, *Testosterone - Additive) tube. Ship refrigerated or frozen. Morning CLIA, LC-MS/MS pm; Report 2 MALES LC-MS/MS sample preferred. days Prefered sampling HYPOGONADIS *FSH *LH Sample Daily by 4 2 mL (1 mL min) serum from 1 Red Top (No time is Day 2 or Day G202 M PANEL, *Estradiol- LC- CLIA, LC-MS/MS pm; Report 2 Additive) tube. Ship refrigerated or frozen. 3 of menstrual cycle FEMALES MS/MS days in the morning

PAGE 228 OF 399 HYPOTHYROIDIS M / *Free T4 * TSH 4 mL (2 mL min.) serum from 1 SST. Ship Z797 CLIA Daily HASHIMOTO'S *Anti TPO refrigerated or frozen. DISEASE PANEL Sample by 7th of 2 mL (1 mL min.) serum from 1 SST. Ship the month; RW103 IgA SUBCLASSES Nephelometry refrigerated or frozen. Reportafter 2–3 weeks Sample by 7th of IGF BINDING 2 mL (1 mL min.) serum from 1 Red Top (No the month; RW104 PROTEIN–1; Additive) tube. Ship refrigerated or frozen. Radioimmunoassay Reportafter 2–3 IGFBP–1 Overnight fasting is mandatory. weeks IGF BINDING Sample by Mon / 2 mL (1 mL min.) serum from 1 SST. Ship frozen. DO Chemiluminescent Also see GROWTH R105 PROTEIN - 3; Thu 4 pm; NOT THAW. Immunoassay DISORDER PANEL. IGFBP - 3 ReportSame day Sample by 7th of IGF BINDING 2 mL (1 mL min.) serum from 1 Red Top (No the month; RW105 PROTEIN - 2; Additive) tube. Ship refrigerated or frozen. Radioimmunoassay Report after 2–3 IGFBP - 2 Overnight fasting is preferred. weeks IGF - I & GROWTH 3 mL (1.5 mL min.) serum from 1 SST. Ship frozen. Chemiluminescent Also see GROWTH Z176 Daily HORMONE DO NOT THAW. Overnight fasting is mandatory. Immunoassay DISORDER PANEL. PANEL IGF - I; 2 mL (1 mL min.) serum from 1 SST. Ship frozen. DO Chemiluminescent Also see GROWTH R106 SOMATOMEDIN Daily NOT THAW. Immunoassay DISORDER PANEL. - C IgVH GENE Sample by Wed 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS154 MUTATION PCR Sequencing 11 am; Report 8 (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. DETECTION days

PAGE 229 OF 399 IMATINIB 4 mL (2 mL min.) Whole blood in 1 Lavender Top RESISTANCE (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Sample by Mon N073 PCR, Sequencing MUTATION Submit latest BCR-ABL Quantitative report along 11 am; Report Sat ANALYSIS; IRMA with sample.

1 drop of heel / finger prick blood each on 3 spots of IMD PANEL, *Amino Acids Sample by Mon / filter paper available from LPL. Clinical details and Tandem Mass G135 QUANTITATIVE, *Organic Acids Wed / Fri 4 drug history must accompany sample. Ship Spectrometry BLOOD *Fatty Acids pm;Report 2 days refrigerated or frozen.

3 mL (1.5 mL min.) serum from 1 Red Top (No Sample by 7th of Includes Additive) tube. Do not use SST gel barrier tubes. the month; RW107 IMIPRAMINE metabolite LC-MS/MS Ship refrigerated or frozen. Recommended Reportafter 2–3 Desipramine. specimen: >12 hours post oral dose. weeks

IMMATURE 3 mL (1 mL min.) whole blood in 1 Lavender Fluorescent Flow H250 PLATELET Top (EDTA) tube. Ship refrigerated. DO NOT Daily Cytometry FRACTION (IPF) FREEZE. 2 mL (1 mL min.) serum from 1 Red Top (No IMMUNE Sample by 7th of Additive) tube. Separate serum and freeze within 1 COMPLEX Enzyme the month; RW108 hour of collection. Ship frozen. DO NOT THAW. Do DETECTION BY Immunoassay Reportafter 2–3 not transport in glass tubes. Do not use SST gel C1q BINDING weeks barrier tubes.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J132 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 C4d room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 230 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J096 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 5 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J174 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 21 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

3 mL (2 mL min.) whole blood in 1 Lavender Top *CD3 *CD4 (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 This is the IMMUNE Sample Daily by 9 *CD8Includes % Green Top (Sodium Heparin) tube. Ship immediately recommended test Z147 DEFICIENCY Flow Cytometry am; Report Same and Absolute at 18–22°C. DO NOT REFRIGERATE OR FREEZE. forCD4 / CD8 Counts PANEL 2; Day Counts Specify time, date and clinical details on test and Ratio. request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top IMMUNE (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 Sample Daily by 9 DEFICIENCY Includes % and Green Top (Sodium Heparin) tube. Ship immediately Z148 Flow Cytometry am; Report Same PANEL 3; CD 3 Absolute Counts. at 18-22°C. DO NOT REFRIGERATE OR FREEZE. Day COUNT Specify time, date and clinical details on test request form.

PAGE 231 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top IMMUNE *CD3 (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 Sample Daily by 9 DEFICIENCY *CD4Includes % Green Top (Sodium Heparin) tube. Ship immediately Z219 Flow Cytometry am; Report Same PANEL 4; CD 4 and Absolute at 18-22°C. DO NOT REFRIGERATE OR FREEZE. Day COUNTS Counts Specify time, date and clinical details on test request form. 3 mL (2 mL min.) whole blood in 1 Lavender Top *CD3 (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 IMMUNE Sample Daily by 9 *CD19Includes Green Top (Sodium Heparin) tube. Ship immediately Z220 DEFICIENCY Flow Cytometry am; Report Same % and Absolute at 18-22°C. DO NOT REFRIGERATE OR FREEZE. PANEL 5 Day Counts. Specify time, date and clinical details on test request form.

Includes qualitative identification of IgA/IgG/IgM Heavy Chains and Kappa/ IMMUNOFIXATI 2 mL (1 mL min.) serum from 1 SST. Separate serum Lambda Light Agarose Gel Sample Daily by 9 ON within 1 hour of collection. Ship refrigerated or Chains. Does not E005 Electrophoresis and am; Report Next ELECTROPHORE frozen. Grossly hemolysed specimens are not include Immunofixation day SIS (IFE), SERUM acceptable. identification of IgD/IgE Heavy Chains. For IgD/IgE Heavy Chain typing, use test code E012.

For qualitative identification of IgD 2 mL (1 mL min.) serum from 1 SST. Separate serum IMMUNOGLOB Agarose Gel or IgE Heavy Chains. Sample Daily by 9 within 1 hour of collection. Ship refrigerated or E012 ULIN IgD & IgE Electrophoresis and To be used as a am; Report Next frozen. Grossly hemolysed specimens are not TYPING, SERUM Immunofixation follow-up to test day acceptable. codes E005 or E006, when indicated.

PAGE 232 OF 399 For qualitative identification of IgD 20 mL (10 mL min.) aliquot of 24-hour urine without IMMUNOGLOB Agarose Gel or IgE Heavy Chains. preservative. Record 24 hour volume on test Sample Daily by 9 E013 ULIN IgD & IgE Electrophoresis and To be used as a request form and urine container. Ship refrigerated am; Report 4 days TYPING, URINE Immunofixation follow-up to test or frozen. codes E005 or E006, when indicated Sample by 7th of IMMUNOGLOB 2 mL (1 mL min.) serum from 1 Red Top (No the month; RW244 ULIN IgD, Nephelometry Additive) tube. Ship refrigerated or frozen. Reportafter 2–3 SERUM weeks IMMUNOGLOB Also see ALLERGY 2 mL (1 mL min.) serum from 1 SST. Overnight S028 ULIN IgE, ImmunoCAP tests & HISTAMINE Daily fasting is preferred. Ship refrigerated or frozen. SERUM tests. Sample by 7th of IMMUNOGLOB 3 mL (1.5 mL min.) serum from 1 SST. Ship the month; RW109 ULIN IgG Nephelometry refrigerated or frozen. Overnight fasting is preferred. Reportafter 2–3 SUBCLASSES weeks Sample Mon IMMUNOGLOB 2 mL (1 mL min.) Cerebrospinal fluid in a sterile through Sat by 4 B091 Nephelometry ULIN IgG, CSF screw capped container. Ship refrigerated or frozen. pm;Report Same day

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J153 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 1a room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 233 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J097 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 10 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

3 mL (2 mL min.) whole blood in 1 Lavender Top *CD3 *CD4 *CD8 (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 IMMUNE *CD19 Sample Daily by 9 Green Top (Sodium Heparin) tube. Ship immediately Z146 DEFICIENCY *CD16+/CD56 Flow Cytometry am; Report Same at 18–22°C. DO NOT REFRIGERATE OR FREEZE. PANEL 1 Includes % and Day Specify time, date and clinical details on test Absolute Counts. request form.

Includes qualitative identification of IgA/IgG/IgM Heavy Chains and Kappa/ IMMUNOFIXATI 20 mL (10 mL min.) aliquot of 24-hour urine without Lambda Light ON Agarose Gel preservative. Record 24 hour volume on test Chains. Does not Sample Daily by 9 E006 ELECTROPHORE Electrophoresis and request form and urine container. Ship refrigerated include am; Report 4 days SIS (IFE), 24- Immunofixation or frozen. identification of HOUR URINE IgD/IgE Heavy Chains. For IgD/IgE Heavy Chain typing, use test code E013.

IMMUNOGLOB 2 mL (1 mL min.) serum from 1 SST. Ship B078 ULIN IgA, Immunoturbidimetry Daily refrigerated or frozen. SERUM

PAGE 234 OF 399 IMMUNOGLOB Sample by 2 mL (1 mL min.) serum from 1 SST. Ship B190 ULIN IgG Nephelometry Mon/Thu 4 pm; refrigerated or frozen. Overnight fasting is preferred. SUBCLASS 4 ReportSame day

Also see MULTIPLE SCLEROSIS PANELs; MYELIN- ASSOCIATED 2 mL (1 mL min.) Cerebrospinal fluid in a sterile GLYCOPROTEIN; IMMUNOGLOB screw capped container AND 2 mL (1 mL min.) MYELIN- Sample Daily by 4 ULIN IgG BCG, Nephelometry, Z180 serum from 1 SST. The collection date and time ASSOCIATED pm; Report Next SYNTHESIS Immunoturbidimetry must be the same for both specimens. Ship GLYCOPROTEIN- day INDEX & RATE refrigerated or frozen. SULFATED GLUCURONIC PARAGLOBOSIDE AUTO-ANTIBODY, IgM. Sample by 7th of IMMUNOGLOB 2 mL (1 mL min.) Cerebrospinal fluid in a sterile Fixed Time the month; RW111 ULIN PROFILE, *IgG *IgA *IgM screw capped container. Ship refrigerated or frozen. Nephelometry Reportafter 2–3 CSF Overnight fasting is preferred. weeks IMMUNOGLOB 2 mL (1 mL min.) serum from 1 SST. Ship Z178 ULIN PROFILE, *IgG *IgA *IgM Immunoturbidimetry Daily refrigerated or frozen. SERUM

PAGE 235 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J150 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 ANY 3 MARKERS room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J076 sections on Poly-L-Lysine Coated Slides. Ship at ACTIN (Smooth ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the Muscle Actin) daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J137 sections on Poly-L-Lysine Coated Slides. Ship at AFP (Alpha ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the Feto Protein) daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 236 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J180 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 days AMACR room temperature. Provide a copy of the

Histopathology / Cytopathology report. Indicate site Tissues : 7 days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J043 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 HER-2 / ERBB2 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J029 CHEMISTRY, CD sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 45 (LCA) room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 237 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J157 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CK 5 / 6 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J127 sections on Poly-L-Lysine Coated Slides. Ship at CYTOKERATIN ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the 20 daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J107 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 FSH room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 238 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J044 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 p53 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J045 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 pS2 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Sample by 7th of IMMUNOGLOB 2 ml (1 mL min.) Cerebrospinal fluid in a sterile the month; RW110 Rate Nephelometry ULIN IgM, CSF screw capped container. Ship refrigerated or frozen. Reportafter 2–3 weeks IMMUNOGLOB 2 mL (1 mL min.) serum from 1 SST. Ship B079 ULIN IgM, Immunoturbidimetry Daily refrigerated or frozen. SERUM

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J151 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 ANY 5 MARKERS room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 239 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Use test code J113 Block/Sections/S Z700 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at Imunohistochemistry forPhotomicrograph mears : 5 CUSTOM PANEL room temperature. Provide a copy of the daysTissues : 7 Histopathology/ Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J196 sections on Poly-L-Lysine Coated Slides. Ship at Ber-Ep4 ry forPhotomicrograph mears : 5 days room temperature. Provide a copy of the (EpCAM) Histopathology / Cytopathology report. Indicate site Tissues : 7 days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J143 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 34-BETA E12 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 240 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J186 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CALDESMON room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J141 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CALRETININ room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J163 CHEMISTRY, CD sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 4 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 241 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J100 CHEMISTRY, CD sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 34 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J037 sections on Poly-L-Lysine Coated Slides. Ship at CD 45 Ro (PAN ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the T) daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J065 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 79a room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 242 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J102 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 99 (MIC-2) room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J146 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 138 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J111 sections on Poly-L-Lysine Coated Slides. Ship at CHROMOGRANI ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the N A daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 243 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J187 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CDX-2 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J172 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 IgG room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J046 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 Ki67 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 244 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J128 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 TdT room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J178 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 BCL-6 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J158 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CALCITONIN room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 245 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J041 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CATHEPSIN D room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J066 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 3 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J062 CHEMISTRY, CD sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 20 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 246 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J175 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 35 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J101 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 117 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J130 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CYCLIN-D1 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 247 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 IMMUNOHISTO Formalin fixed paraffin embedded block OR Alcohol pmReport CHEMISTRY, fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J017 CYTOKERATIN sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 (PANCYTOKERA room temperature. Provide a copy of the daysTissues : 7 TIN) Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J188 sections on Poly-L-Lysine Coated Slides. Ship at CYTOKERATIN ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the 19 daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J167 sections on Poly-L-Lysine Coated Slides. Ship at CYTOMEGALOVI ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the RUS (CMV) daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 248 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 IMMUNOHISTO Formalin fixed paraffin embedded block OR Alcohol pmReport CHEMISTRY, fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J030 EPITHELIAL sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 MEMBRANE room temperature. Provide a copy of the daysTissues : 7 ANTIGEN (EMA) Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR IMMUNOHISTO Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol CHEMISTRY, pmReport fixed Fine Needle Aspirate Smears OR 5 unstained GLIAL Immunohistochemist Use test code J113 Block/Sections/S J034 sections on Poly-L-Lysine Coated Slides. Ship at FIBRILLARY ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the ACIDIC daysTissues : 7 Histopathology / Cytopathology report. Indicate site PROTEIN (GFAP) days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J199 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 GLYPICAN - 3 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 249 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J105 sections on Poly-L-Lysine Coated Slides. Ship at GROWTH ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the HORMONE daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR IMMUNOHISTO Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol CHEMISTRY, pmReport fixed Fine Needle Aspirate Smears OR 5 unstained HUMAN Immunohistochemist Use test code J113 Block/Sections/S J191 sections on Poly-L-Lysine Coated Slides. Ship at CHORIONIC ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the GONADOTROPI daysTissues : 7 Histopathology / Cytopathology report. Indicate site N (HCG) days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J138 sections on Poly-L-Lysine Coated Slides. Ship at LAMBDA LIGHT ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the CHAINS daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

3 mL (2 mL min.) Whole blood in 1 Lavender Top IMMUNOSUPPR (EDTA) tube. Ship refrigerated . Ideal sampling time: Sample Daily by 5 *Tacrolimus G169 ESSANT DRUG Just before the next dose (Trough levels). Provide LC-MS/MS pm; Report Same *Sirolimus PROFILE 1 date & time of sampling, dose taken & time of Day dosing.

PAGE 250 OF 399 3 mL (2 mL min.) Whole blood in 1 Lavender Top IMMUNOSUPPR (EDTA) tube. Ship refrigerated .Ideal sampling time: Sample Daily by 5 *Tacrolimus G168 ESSANT DRUG Just before the next dose (Trough levels). Provide LC-MS/MS pm; Report Same *Everolimus PROFILE 4 date & time of sampling, dose taken & time of Day dosing.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J140 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 INHIBIN room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J159 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 p63 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 IMMUNOHISTO Formalin fixed paraffin embedded block OR Alcohol pmReport CHEMISTRY, fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J093 PROSTATE sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 SPECIFIC room temperature. Provide a copy of the daysTissues : 7 ANTIGEN (PSA) Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 251 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 IMMUNOHISTO Formalin fixed paraffin embedded block OR Alcohol pmReport CHEMISTRY, fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J166 SERUM sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 ASSOCIATED room temperature. Provide a copy of the daysTissues : 7 AMYLOID (SAA) Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J067 sections on Poly-L-Lysine Coated Slides. Ship at THYROGLOBULI ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the N daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

3 mL (2 mL min.) Whole blood in 1 Lavender Top IMMUNOSUPPR (EDTA) tube. Ship refrigerated . Ideal sampling time: Sample Daily by 5 *Cyclosporine G167 ESSANT DRUG Just before the next dose (Trough levels). Provide LC-MS/MS pm; Report Same *Tacrolimus PROFILE 3 date & time of sampling, dose taken & time of Day dosing.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J126 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CYTOKERATIN 7 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 252 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 IMMUNOHISTO Formalin fixed paraffin embedded block OR Alcohol pmReport CHEMISTRY, fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J160 HEPATOCYTE sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 SPECIFIC room temperature. Provide a copy of the daysTissues : 7 ANTIGEN (HSA) Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% formal-saline OR Sample Daily by 6 IMMUNOHISTO Formalin Fixed paraffin embedded block. Ship at pmReport Immunohistochemist J192 CHEMISTRY, INI- room temperature.Provide a copy of the Block:5 ry 1 Histopathology / Cytopathology report. Indicate site daysReport of Biopsy and proivde clinical history Tissue: 7 Days

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J189 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 MELAN-A room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J142 sections on Poly-L-Lysine Coated Slides. Ship at MYELOPEROXID ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the ASE daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 253 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J190 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 MYOGENIN room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 IMMUNOHISTO Formalin fixed paraffin embedded block OR Alcohol pmReport CHEMISTRY, fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J036 NEURON sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 SPECIFIC room temperature. Provide a copy of the daysTissues : 7 ENOLASE (NSE) Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J179 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 PAX -5 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 254 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J010 sections on Poly-L-Lysine Coated Slides. Ship at PROGESTERONE ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the RECEPTOR (PR) daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J031 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 S100 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J193 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 UROPLAKIN - II room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 255 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J144 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 TTF-1 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J136 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 WT-1 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

3 mL (2 mL min.) Whole blood in 1 Lavender Top IMMUNOSUPPR (EDTA) tube. Ship refrigerated . Ideal sampling time: Sample Daily by 5 *Cyclosporine G166 ESSANT DRUG Just before the next dose (Trough levels). Provide LC-MS/MS pm; Report Same *Sirolimus PROFILE 2 date & time of sampling, dose taken & time of Day dosing.

IMMUNOHISTO Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 CHEMISTRY, Formalin fixed paraffin embedded block OR Alcohol pmReport EPIDERMAL fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J077 GROWTH sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 FACTOR room temperature. Provide a copy of the daysTissues : 7 RECEPTOR Histopathology / Cytopathology report. Indicate site days (EGFR) of biopsy and provide Clinical history.

PAGE 256 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J103 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 HMB 45 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J139 sections on Poly-L-Lysine Coated Slides. Ship at KAPPA LIGHT ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the CHAINS daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J125 sections on Poly-L-Lysine Coated Slides. Ship at SYNAPTOPHYSI ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the N daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 257 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J032 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 VIMENTIN room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J018 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 DESMIN room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport fixed Fine Needle Aspirate Smears OR 5 unstained IMMUNOHISTO Immunohistochemist Use test code J113 Block/Sections/S J171 sections on Poly-L-Lysine Coated Slides. Ship at CHEMISTRY, IgA ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 258 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J061 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 15 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J099 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 31 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J064 CHEMISTRY, CD sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 68 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 259 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J108 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 ACTH room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J094 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 BCL-2 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% formal-saline OR Sample Daily by 6 IMMUNOHISTO Formalin Fixed paraffin embedded block. Ship at pmReport Immunohistochemist J195 CHEMISTRT, C- room temperature.Provide a copy of the Block:5 ry MYC Histopathology / Cytopathology report. Indicate site daysReport of Biopsy and proivde clinical history Tissue: 7 Days

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J098 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 23 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 260 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J063 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CD 30 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J161 CHEMISTRY, CD sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 56 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J145 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 CEA room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Specimen: Submit tumour tissue in 10% formal- Sample Daily by 6 IMMUNOHISTO saline OR Formalin Fixed paraffin embedded block. Immunohistochemist pmReport Blocks J194 CHEMISTRY, Ship at room temperature.Provide a copy of the ry :5 DaysTissues : 7 BETA-CATENIN Histopathology / Cytopathology report. Indicate site Days of Biopsy and proivde clinical history.

PAGE 261 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J173 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 DOG-1 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J197 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 GATA-3 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

IMMUNOGLOB 2 mL (1 mL min.) serum from 1 SST. Ship B077 ULIN IgG, Immunoturbidimetry Daily refrigerated or frozen. SERUM

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J147 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 days ALK-1 room temperature. Provide a copy of the

Histopathology / Cytopathology report. Indicate site Tissues : 7 days of biopsy and provide Clinical history.

PAGE 262 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J162 CHEMISTRY, CD sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 8 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J033 sections on Poly-L-Lysine Coated Slides. Ship at NEUROFILAMEN ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the T POLYPEPTIDE daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J181 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 OCt 2 room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

PAGE 263 OF 399 Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol IMMUNOHISTO pmReport fixed Fine Needle Aspirate Smears OR 5 unstained CHEMISTRY, Immunohistochemist Use test code J113 Block/Sections/S J148 sections on Poly-L-Lysine Coated Slides. Ship at POLYOMA BK ry forPhotomicrograph mears : 5 room temperature. Provide a copy of the VIRUS (SV 40) daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 Formalin fixed paraffin embedded block OR Alcohol pmReport IMMUNOHISTO fixed Fine Needle Aspirate Smears OR 5 unstained Immunohistochemist Use test code J113 Block/Sections/S J104 CHEMISTRY, sections on Poly-L-Lysine Coated Slides. Ship at ry forPhotomicrograph mears : 5 PROLACTIN room temperature. Provide a copy of the daysTissues : 7 Histopathology / Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 IMUNOHISTOCH Formalin fixed paraffin embedded block OR Alcohol pmReport EMISTRY, fixed Fine Needle Aspirate Smears OR 10 unstained *MLH-1 *MSH-2 Immunohistochemist Use test code J113 Block/Sections/S Z680 MICROSATELLIT sections on Poly-L-Lysine Coated Slides. Ship at *MSH-6 *PMS-2 ry forPhotomicrograph mears : 5 E INSTABILITY room temperature. Provide a copy of the daysTissues : 7 PANEL Histopathology/Cytopathology report. Indicate site days of biopsy and provide Clinical history.

Submit tumour tissue in 10% formal-saline OR Sample Daily by 6 IMUNOHISTOCH Formalin Fixed paraffin embedded block. Ship at pmReport Immunohistochemist J198 EMISTRY, room temperature.Provide a copy of the Block:5 ry NAPSIN-A Histopathology / Cytopathology report. Indicate site daysReport of Biopsy and proivde clinical history Tissue: 7 Days

PAGE 264 OF 399 Also see CULTURE, INDIA INK FUNGUS and P068 PREPARATION, 1 mL (0.5 mL min.) Fluid. Ship at 18–22°C. Light Microscopy FUNGUS Daily FLUID EXAMINATION, ROUTINE Also see CULTURE, INDIA INK FUNGUS and P051 PREPARATION, 1 mL (0.5 mL ) Cerebrospinal fluid. Ship at 18-22°C. Light Microscopy FUNGUS Daily CSF EXAMINATION, ROUTINE *FSH *LH *Prolactin In females, INFERTILITY *Testosterone, 4 mL (2 mL min.) serum from 1 SST. Collect preferred sampling Sample Daily by 9 COMPREHENSIV Total * TSH specimen 4 hrs after the patient has awakened. Chemiluminescent Z812 time is Day 2 / Day 3 am; Report Same E PANEL, Ultrasensitive * Overnight fasting is preferred .Ship refrigerated or Immunoassay, EIA of the menstrual day FEMALES Estradiol * Anti frozen. cycle. Mullerian Hormone (AMH) *FSH *LH INFERTILITY *Testosterone, 3 mL (1.5 mL min.) serum from 1 SST. Ship Chemiluminescent Z605 Daily PANEL, MALES Total *TSH refrigerated or frozen. Immunoassay Ultrasensitive *FSH *LH In females, INFERTILITY 3 mL (1.5 mL min.) serum from 1 SST. Collect *Prolactin preferred sampling SCREENING specimen 4 hrs after the patient has awakened. Chemiluminescent Z179 *Testosterone, time is Day 2 / Day 3 Daily PANEL, Overnight fasting is preferred. Ship refrigerated or Immunoassay Total * TSH of the menstrual FEMALES frozen. Ultrasensitive cycle. Day 2 / 3 sample of the menstrual cycle Sample by Mon 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme F025 INHIBIN B is preferred to am; Report Same refrigerated or frozen. Immunoassay assess Ovarian day reserve.

PAGE 265 OF 399 INHIBIN A, Sample Daily by 9 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent F022 REPRODUCTIVE am; Report Same refrigerated or frozen. Immunoassay MARKER Day

Sample Daily by 9 INHIBIN A, 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent F021 am; Report Same PREGNANCY refrigerated or frozen. Immunoassay Day

IN-SITU- Submit tumour tissue in 10% Formal-saline OR Sample Daily by 6 HYBRIDIZATION Formalin fixed paraffin embedded block Ship at pmReport Block : J185 - EPSTEIN BARR room temperature. Provide a copy of the In-Situ-Hybridization 5 daysTissues : 7 VIRUS EARLY Histopathology / Cytopathology report. Indicate site days RNA (EBER-ISH) of biopsy and provide Clinical history. Sample by Sat 9 INSULIN 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S133 am; Report Same ANTIBODIES refrigerated or frozen. Immunoassay day 2 mL (1 mL min.) serum from 1 SST. Samples to be Chemiluminescent Also see INSULIN R042 INSULIN, PP collected 2 hours post meal. Ship refrigerated or MicroparticleImmun RESPONSE TO Daily frozen. oassay GLUCOSE Test INSULIN, FASTING (F) & 2 mL (1 mL min.) serum from 1 SST each for F & PP Chemiluminescent Also see INSULIN Z385 POST samples. Overnight fasting is mandatory. Ship MicroparticleImmun RESPONSE TO Daily PRANDIAL (PP), refrigerated or frozen. oassay GLUCOSE Test PANEL Chemiluminescent Also see NSULIN INSULIN, 2 mL (1 mL min.) serum from 1 SST. Ship R041 MicroparticleImmun RESPONSE TO Daily RANDOM refrigerated or frozen. oassay GLUCOSE Test INSULIN Sample by 7th of 2 mL (1 mL min.) serum from 1 Red Top (No ANTIBODIES, the month; RW112 Additive) tube. Separate serum within 1 hour of Radio binding Assay HIGHLY Reportafter 2–3 collection. Ship refrigerated or frozen. SENSITIVE weeks

PAGE 266 OF 399 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium Fluoride) tube AND 2 mL (1 mL min.) Refer to Y corresponding serum from 1 SST for each timed Codes:Y084 specimen set. Draw baseline fasting specimens. BasalY085 0.5 INSULIN Dissolve 75g Glucose in adults / 1.75 g / Kg body Chemiluminescent hrY086 1.0 hrY087 RESPONSE TO weight in children (not exceeding 75g) in 300mL Z084 MicroparticleImmun 1.5 hrY088 2.0 Daily GLUCOSE, 5 water. Administer orally over a period of 5 minutes. oassay, Hexokinase hrY089 2.5 hrY090 HOURS Draw additional samples at 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 hrY091 4.0 3.0, 4.0 and 5.0 hours. Note time drawn and tests hrY092 5.0 hrY093 required on each container label and test request Customized form. Overnight fasting mandatory.Ship refrigerated or frozen. Urine specimens NOT REQUIRED.

The free (bioactive) insulin test is Sample by 7th of 2 mL (1 mL min.) serum from 1 Red Top (No INSULIN, FREE recommended for the month; RW113 Additive) tube. Overnight fasting is mandatory. Ship Immunoassay (BIOACTIVE) patients who have Reportafter 2–3 refrigerated or frozen. detectable levels of weeks insulin antibodies.

Chemiluminescent Also see INSULIN INSULIN, 2 mL (1 mL min.) serum from 1 SST. Overnight R039 MicroparticleImmun RESPONSE TO Daily FASTING fasting is mandatory. Ship refrigerated or frozen. oassay GLUCOSE Test

PAGE 267 OF 399 Cytokine levels may demonstrate diurnal variation. 2 mL (1 mL min.) serum from 1 Red Top (No Recommend Sample by 7th of Additive) tube OR 2 mL (1 mL min.) plasma from 1 INTERLEUKIN - Enzyme cytokine levels be the month; RW115 Lavender Top (EDTA) tube. Separate serum / plasma 6; IL-6 Immunoassay determined at the Reportafter 2–3 within 2 hours of collection in a plastic transport vial same time of day for weeks and freeze immediately. Ship frozen. DO NOT THAW. improved longitudinal comparison. 2 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th of Additive) tube. Collect sample at least 8 hours after Multianalyte INTERFERON - the month; RW256 Interferon injection. Verify medication and provide Immunodetection BETA IgG, MAID Reportafter 2–3 dose & duration of therapy. Ship refrigerated or (MAID) weeks frozen. 2 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th of INTERLEUKIN - Additive) tube. Separate serum within 1 hour of Enzyme the month; RW114 2 RECEPTOR; collection in a plastic transport vial and freeze Immunoassay Reportafter 2–3 IL-2R immediately. Ship frozen. DO NOT THAW. weeks INTERLEUKIN 28B rs12979860 & Sample by Thu / 3 mL (2 mL min.) whole blood from 1 Lavender Top N050 rs 8099917 Real Time PCR Mon 9 am; (EDTA) tube. Ship refrigerated. DO NOT FREEZE. GENOTYPING, ReportSat / Wed QUALITATIVE, PCR INTRINSIC Sample by Mon 9 2 mL (1 mL min.) serum from 1 SST. Ship S244 FACTOR EIA am; report Same refrigerated or frozen. ANTIBODY day

PAGE 268 OF 399 Inv 16 (p13q22) / t(16;16)(p13;q2 3 mL (2 mL min.) Whole blood / Bone Marrow from Sample by Mon / 2) GENE N046 1 Lavender Top (EDTA) tube. Ship refrigerated. DO Real Time PCR Thu 11 am; REARRANGEME NOT FREEZE. Clinical History is mandatory. Report Wed / Sat NT QUALITATIVE, PCR *SERUM IRON *TOTAL IRON 3 mL (1.5 mL min ) serum from 1 SST. Avoid BINDING hemolysis. Ship refrigerated or frozen. Overnight H020 IRON STUDIES Spectrophotometry Also see FERRITIN. Daily CAPACITY * fasting is preferred. Avoid iron supplements for TRANSFERRIN minimum 7 days prior to specimen collection. SATURATION) *Serum Iron *Total Iron 4 mL (1.5 mL min ) serum from 1 SST. Avoid IRON STUDIES Binding Capacity hemolysis. Ship refrigerated or frozen. Overnight Spectrophotometry, Z783 MONITORING Daily *Transferrin fasting is preferred. Avoid iron supplements for CLIA PANEL Saturation minimum 7 days prior to specimen collection. *Ferritin

Indirect Sample Daily by ISLET CELL 2 mL (1 mL min.) serum from 1 SST. Ship S134 Immunofluorescence 11 am; Report ANTIBODY refrigerated or frozen. Assay Same day

ISLET CELL Indirect Sample Daily by 2 mL (1.5 mL min.) serum from 1 SST. Ship S186 ANTIBODY, IN Immunofluorescence 11 am; Report refrigerated or frozen. DILUTIONS Assay Next day

JAK 2 EXON 12, 3 mL (2 mL min.) whole blood from 1 Lavender Top Sample by Mon / PCR, Fragment N122 MUTATION (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Thu 11 am; Analysis DETECTION Give brief clinical history. ReportWed / Sat

PAGE 269 OF 399 JAK 2 MUTATION 3 mL (2 mL min.) whole blood from 1 Lavender Top Sample by Mon / N039 DETECTION, (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Real Time PCR Thu 11 am; QUALITATIVE Clinical history is mandatory. ReportWed / Sat PCR JAK 2 V617F REFLEX TO JAK 3 mL (2 mL min.) whole blood from 1 Lavender Top Real Time PCR, Sample by Mon Z839 2 EXON 12, (EDTA) tube. Ship refrigerated. DO NOT FREEZE. 0 Fragment Analysis 11 am; ReportSat MUTATION Give brief clinical history DETECTION JAK 2 V617F, CALR & MPL, 3 mL (2 mL min.) whole blood from 1 Lavender Top Real Time PCR, Sample by Mon Z840 MUTATION (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Sequencing, 0 11 am; ReportSat DETECTION Give brief clinical history Fragment Analysis PROFILE JAPANESE ENCEPHALITIS Sample by Wed 3 mL (2 mL min.) CSF in a sterile screw capped OS157 VIRUS Real Time PCR 11 am; Report 7 container. Ship refrigerated or frozen. DETECTION , days PCR 4 mL (2 mL min.) Serum from 1 SST OR 4 mL (2 mL JC/BK VIRUS min.) plasma from 1 Lavendar top (EDTA) tube OR 3 Sample by OS156 DETECTION , mL (2 mL min.) CSF / Urine in a sterile screw capped PCR Wed/Sat 11 am; PCR container OR Tissue in normal saline. Ship Report 6 days refrigerated or frozen. Also see Sample by Tue / 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S080 Jo-1 ANTIBODY EXTRACTABLE Fri 9 am; Report refrigerated or frozen. Immunoassay NUCLEARANTIGENS. Same day

PAGE 270 OF 399 *Kappa Light KAPPA / Sample Mon Chains * Lambda LAMBDA LIGHT 2 mL (1 mL min.) serum from 1 SST. Ship through Sat by 4 B148 Light chains* Nephelometry CHAINS, FREE, refrigerated or frozen. pm; Report Same Kappa Lambda SERUM day Ratio KAPPA / Sample Mon *Kappa Light 10 mL (5 mL min.) aliquot of random urine in a LAMBDA LIGHT through Sat by 4 U079 Chains* Lambda sterile screw capped container. Ship refrigerated or Nephelometry CHAINS, FREE, pm; Report Same Light chains frozen. URINE day 20 mL (10 mL min.) well mixed aliquot of 24-hour 17 – urine. Add appropriate volume of 50% HCl to Sample Daily by 4 KETOSTEROIDS, Column U005 maintain pH below 5. Record 24 hour urine volume pm; Report 3 24–HOUR Chromatography on test request form and container. Ship days URINE refrigerated or frozen.

*Glucose R *Creatinine *Uric Acid *Cholesterol*Pro 3 mL (1.5 mL min.) serum from 1 SST AND 2 mL (1 tein Total mL min.) plasma from 1 Grey Top (Sodium Fluoride) *Albumin *A:G tube AND 3 mL (1 mL min.) whole blood in 1 Spectrophotometry, KIDNEY Z379 Ratio *Alkaline Lavender Top (EDTA) tube. Avoid use of tourniquet Indirect ISE, Daily DIALYSIS PANEL Phosphatase and fist clenching during sampling. Overnight fasting Hexokinase *Calcium is preferred AND 10 mL (5 mL min.) aliquot of first *Phosphorus morning urine. Ship refrigerated. DO NOT FREEZE. *Electrolytes, serum *BUN *CBC *Urine R/E

PAGE 271 OF 399 *Urea *Creatinine *Uric Acid *Protein Total *Albumin *A:G 4 mL (1.5 mL min ) serum from 1 SST AND 2 mL (1 Ratio *Alkaline mL min.) plasma from 1 Grey Top (Sodium Fluoride) Spectrophotometry, KIDNEY PANEL Phosphatase Z784 tube. Avoid use of tourniquet and fist clenching Hexokinase, Indirect Daily EXTENDED *Calcium during sampling. Avoid hemolysis. Overnight fasting ISE *Phosphorus is preferred. Ship refrigerated or frozen. *Electrolytes, serum *Bicarbonate *Glucose Random *Urea *Creatinine *Uric Acid *Protein Total*Albumin 3 mL (1.5 mL min.) serum from 1 SST. Avoid use of KIDNEY PANEL; *A:G Ratio tourniquet and fist clenching during sampling. Spectrophotometry, Z007 Daily KFT *Alkaline Overnight fasting is preferred. Ship refrigerated or Indirect ISE Phosphatase frozen. *Calcium *Phosphorus *Electrolytes, serum

PAGE 272 OF 399 Day 1: 50 mL (30 mL min.) aliquot of 24-hour urine. *pH *Creatinine Do not use preservatives. Mention total volume on *Calcium Test Request Form & label container as Non- KIDNEY STONE *Magnesium acidified urine.Day 2: 50 mL (30 mL min.) aliquot of Spectrophotometry, Sample by Wed / FORMATION, *Citrate*Oxalate 24 hour urine. Collect urine with 25 mL 50% Z230 Enzymatic, Indirect Sat 9 am; Report DIAGNOSTIC *Osmolality concentrated HCl to maintain pH below 3. Mention ISE Same day PANEL *Electrolytes, total volume on Test Request Form & label urine *Uric Acid container as Acidified urine.Ship both specimens *Phosphorus frozen. DO NOT THAW. Record patient weight on Test Request Form.

Day 1: 50 mL (30 mL min.) aliquot of 24-hour urine. *pH *Creatinine Do not use preservatives. Mention total volume on *Calcium Test Request Form & label container as Non- KIDNEY STONE *Magnesium acidified urine.Day 2: 50 mL (30 mL min.) aliquot of FORMATION, Spectrophotometry, Sample by Wed / *Citrate*Oxalate 24 hour urine. Collect urine with 25 mL 50% Z231 THERAPEUTIC Enzymatic, Indirect Sat 9 am; Report *Osmolality concentrated HCl to maintain pH below 3. Mention MONITORING ISE Same day *Sodium *Uric total volume on Test Request Form & label PANEL Acid container as Acidified urine. Ship both specimens *Phosphorus frozen. DO NOT THAW. Record patient weight on Test Request Form. KRAS CODON Sample by Mon Submit fresh tissue in normal saline OR formalin OS158 61 MUTATION PCR 11 am; Report 10 fixed paraffin embedded block DETECTION days KRAS MUTATION, Submit formalin fixed paraffin embedded tissue PCR, Fragment Sample by Tue 11 N067 CODON 12 & block. Ship at room temperature. Clinical history & analysis am; Report Mon 13 FRAGMENT Histopathology report is mandatory. ANALYSIS

PAGE 273 OF 399 KRAS & NRAS Submit formalin fixed paraffin embedded tissue PCR, Fragment MUTATION Sample by Tue 11 Z816 block. Ship at room temperature. Block should have analysis and ANALYSIS am; Report Mon at least 10% tumor tissue. Sequencing PANEL 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium LACTATE, Separation of Fluoride) tube without applying tourniquet. B113 PLASMA, POST Spectrophotometry plasma at source Daily Separate plasma immediately. Ship refrigerated or EXERCISE ismandatory. frozen. Separation of 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium plasma at source is LACTATE, Fluoride) tube without applying tourniquet. mandatory. Use Test B099 Spectrophotometry Daily PLASMA Separate plasma immediately. Ship refrigerated or Code B113 for frozen. Lactate Stimulation by Exercise. 1 mL (0.5 mLmin) Cerebrospinal fluid. Ship B098 LACTATE, CSF Spectrophotometry Daily refrigerated or frozen. 2 mL (1 mL min.) serum from 1 Red Top ( No Sample by 7th of Additive) tube. Recommended specimen: 0.5–1 the month; RW118 LAMOTRIGINE hour before next dose at steady state. Ship LC-MS/MS Reportafter 2–3 refrigerated or frozen. Do not use SST gel barrier weeks tubes. LDH (LACTATE 5 mL (2 mL min.) fluid in a sterile screw capped P052 DEHYDROGENA IFCC Daily container. Ship refrigerated. DO NOT FREEZE. SE), FLUID LDH; LACTATE 2 mL (1 mL min.) serum from 1 SST. Ship B031 DEHYDROGENA refrigerated. DO NOT FREEZE. Hemolysed IFCC Daily SE specimens are not acceptable. LDL 2 mL (0.5 mL min.) serum from 1 SST. Ship Enzymatic selective Also see LIPID B129 CHOLESTEROL, Daily refrigerated or frozen. protection PROFILE TESTS. DIRECT

PAGE 274 OF 399 2 mL (1.5 mL min.) serum from 1 SST. Separate serum within 1 hour of collection. Ship refrigerated. Polyacrylamide Gel Sample by Fri 9 LDL E014 DO NOT FREEZE. Minimum 12 hours fasting is Electrophoresis(PAG am; Report Same SUBFRACTIONS mandatory. Grossly hemolysed samples are not E) day acceptable.

Use only specified container available from LPL. If 4 mL (2 mL min.) whole blood from 1 metal free Atomic Absorption gadolinium or iodine Sample Daily by 6 Royal Blue Top (K2EDTA) tube available from LPL. Spectrometry containing contrast C020 LEAD, BLOOD pm; ReportNext Ship refrigerated. DO NOT FREEZE. Use withZeeman media has been day powderlessgloves during specimen collection. correction administered, the specimen should not be collected for 96 hours.

Use only specified container available 10 mL (5 mL min.) aliquot of 24 hour urine collected from LPL. If in an acid washed (metal free) container kit Atomic Absorption gadolinium or iodine available from LPL. Mix thoroughly. Transfer 10 mL Sample Daily by 6 LEAD, 24-HOUR Spectrometry containing contrast C021 aliquot to vial provided in kit. Measure 24 hour total pm; ReportNext URINE withZeeman media has been volume and record on vial and Test request form. day correction administered, the Ship refrigerated or frozen. Use powderless gloves specimen should not during specimen collection. be collected for 96 hours.

PAGE 275 OF 399 Use only specified 10 mL (5 mL min.) aliquot of 24 hour urine collected container available in a dark coloured container without preservative from LPL. If Ship frozen. DO NOT THAW. Record 24 hour volume Column gadolinium or iodine LEAD *5-Amino on test request form and urine container. Sample by Tue / chromatography, containing contrast Z038 POISONING Levulinic Acid (5- Refrigerate during collection. AND 4 mL (2 mL min.) Fri 9 am; Report AAS with Zeeman media has been PANEL ALA) *Lead Blood whole blood from 1 metal free Royal Blue Top Next day correction administered, the (K2EDTA) tube available from LPL. Ship refrigerated. specimen should not DO NOT FREEZE. Use powderless gloves during be collected for 96 specimen collection. hours.

Use only specified container available from LPL. If 10 mL (5 mL min.) aliquot of random urine collected Atomic Absorption gadolinium or iodine LEAD, Sample Daily by 6 in an acid washed (metal free) container kit Spectrometry containing contrast C022 RANDOM pm; Report Next available from LPL. Ship refrigerated or frozen. Use withZeeman media has been URINE day powderless gloves during specimen collection. correction administered, the specimen should not be collected for 96 hours.

10 mL (5 mL min.) whole blood in a plastic container Also see Sample Daily by 6 LE CELL Romanowsky H036 with 4 glass beads. Rotate for 30 min to defibrinate. ANTINUCLEAR ANTI pm; Report Next PHENOMENON staining Ship refrigerated. DO NOT FREEZE. BODY/FACTOR. day LEGIONELLA 5 mL aliquot of random urine in a sterile screw Sample Daily by 1 M217 ANTIGEN, capped container. Ship refrigerated only. DO NOT ICT pmReport Same URINE FREEZE. day

PAGE 276 OF 399 Sample by 7th of LEGIONELLA 5 mL (3 mL min.) aliquot of random urine in a sterile the month; RW121 ANTIGEN, Immunoassay screw capped container. Ship refrigerated or frozen. Reportafter 2–3 URINE weeks LEGIONELLA Sample by 7th of PNEUMOPHILA Serogroups 1, 2- 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence the month; RW119 TOTAL 6 & 8 refrigerated or frozen. Assay Reportafter 2–3 ANTIBODY, weeks SERUM LEISHMANIA Sample by Sat 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S025 (KALA AZAR) am; Report Same refrigerated or frozen. Immunoassay ANTIBODY IgG day Sample by Sat 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme B164 LEPTIN am; Report Same refrigerated or frozen. Immunoassay day LEPTOSPIRA Sample by Tue / ANTIBODIES 2 mL (1 mL min) serum from 1 SST. Ship refrigerated Enzyme Z302 Sat 9 am; Report PANEL, IgG & or frozen Immunoassay Same day IgM Sample by Tue / LEPTOSPIRA 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S157 Sat 9 am; Report ANTIBODY, IgG refrigerated or frozen. Immunoassay Same day

Sample by Tue / LEPTOSPIRA 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S197 Sat 9 am; Report ANTIBODY, IgM refrigerated or frozen. Immunoassay Same day 4 mL (2 mL min.) Whole Blood in 1 Lavender top LEPTOSPIRA Sample by Mon / (EDTA) tube OR 10 mL Urine in a sterile screw OS159 DETECTION, PCR Thu 11 am; capped container. Ship refrigerated. DO NOT PCR Report Wed / Sat FREEZE.

PAGE 277 OF 399 LEUCOCYTE Whole blood in ALKALINE 5 fresh blood smears from finger prick, fixed in 95% EDTA tubes not Sample Daily by 9 H050 PHOSPHATASE ethanol for 30 min. Air dry and ship at room Cytochemistry accepted. Only fresh am; Report Next SCORE; LAP temperature immediately. blood smears to be day SCORE sent. LEUKEMIA / LYMPHOMA 3 mL (2 mL min.) whole blood each in 1 Lavender DIAGNOSTIC Top (EDTA) tube AND 1 Green Top (Sodium Heparin) PANEL: tube OR 2 mL (1 mL min.) heparinized Bone Marrow Sample Daily by 9 Z151 CHRONIC AND Aspirate Smear. Ship immediately at 18–22°C. Flow Cytometry am; Report Same LYMPHOPROLIF DO NOT REFRIGERATE OR FREEZE. Specify time, Day ERATIVE date and clinical details on test request form. DISORDERS, T Clinical History Mandatory. & B CELL

*Chromosome analysis for 2 mL (1.5 mL min.) Bone Marrow Aspirate / Whole hematologicalma LEUKEMIA Blood in 1 Green Top (Sodium Heparin) tube AND 3 Culture, lignancy *AML Sample Daily by DIAGNOSTIC mL (2 mL min.) Whole blood / Bone Marrow Microscopy, Z601 categorization 11 am; Report 7 COMPREHENSIV Aspirate in 1 Lavender Top (EDTA) tube. Ship Karyotype, Real by PCR / BCR days E PROFILE 4 refrigerated. DO NOT FREEZE. Give clinical history Time PCR ABL gene on Chromosome Test Request Form . rearrangement, PCR Qualitative

*Chromosome 2 mL (1.5 mL min.) Bone Marrow Aspirate / Whole analysis for LEUKEMIA Blood in 1 Green Top (Sodium Heparin) tube AND 3 Culture, hematological Sample Daily by DIAGNOSTIC ml (2 mL min.) Whole blood / Bone Marrow Microscopy, Z665 malignancy *BCR 11 am; Report 7 COMPREHENSIV Aspirate in 1 Lavender Top (EDTA) tube. Ship Karyotype, Real ABL gene days E PROFILE 6 refrigerated. DO NOT FREEZE. Give clinical history Time PCR rearrangement, on Chromosome Test Request Form. PCR Qualitative

PAGE 278 OF 399 3 mL (2 mL min.) whole blood each in 1 Lavender LEUKEMIA Top (EDTA) tube AND 1 Green Top (Sodium Heparin) DIAGNOSTIC tube OR 2 mL (1 mL min.) heparinized Bone Marrow Sample Daily by 9 PANEL, Z512 AND Aspirate Smear. Ship immediately at 18–22°C. Flow Cytometry am; Report Same CUSTOMIZED DO NOT REFRIGERATE OR FREEZE. Specify time, Day (ANY 10 date and clinical details on test request form. MARKERS) Clinical History Mandatory.

3 mL (2 mL min.) whole blood each in 1 Lavender LEUKEMIA Top (EDTA) tube AND 1 Green Top (Sodium Heparin) DIAGNOSTIC tube OR 2 mL (1 mL min.) heparinized Bone Marrow Sample Daily by 9 Z282 PANEL: AND Aspirate Smear. Ship immediately at 18–22°C. Flow Cytometry am; Report Same CLL/HCL/SLL DO NOT REFRIGERATE OR FREEZE. Specify time, Day (BASIC) date and clinical details on test request form. Clinical History Mandatory.

*Leukemia diagnostic panel, Acute Leukemia - T, B or Myeloid, *Chromosome 2 mL (1.5 mL min.) Bone Marrow Aspirate in 1 Flow Cytometry, LEUKEMIA analysis Green Top (Sodium Heparin) tube AND 3 mL (2 mL Culture, Sample Daily by DIAGNOSTIC forhematological min.) Whole blood / Bone Marrow in 1 Lavender Z538 Microscopy, 11 am; Report 7 COMPREHENSIV malignancy, Top (EDTA) tube. Ship at specified temperatures. Karyotype, Real days E PROFILE 3 *AML Give clinical history on Chromosome Test Request Time PCR categorization Form by PCR / BCR ABL gene rearrangement, PCR Qualitative

PAGE 279 OF 399 3 mL (2 mL min.) whole blood each in 1 Lavender LEUKEMIA Top (EDTA) tube AND 1 Green Top (Sodium Heparin) DIAGNOSTIC tube OR 2 mL (1 mL min.) heparinized Bone Marrow Sample Daily by 9 Z780 PANEL: AND Aspirate Smear. Ship immediately at 18–22°C. Flow Cytometry am; Report Same PLASMA CELL DO NOT REFRIGERATE OR FREEZE. Specify time, Day DYSCRASIAS date and clinical details on test request form. Clinical History Mandatory.

Any 6 of the following Any 6 of the markers can be following markers LEUKEMIA selected - BCR- can be selected - GENETIC 3 mL (2 mL min.) Whole blood / Bone Marrow ABL, PML-RARA, BCR-ABL, PML- Sample by Tue / PROFILE - ANY Aspirate in 1 Lavender Top (EDTA) tube. Ship Z662 AML-ETO, Real Time PCR RARA, AML-ETO, Fri 11 am; Report 6 MARKERS, refrigerated. DO NOT FREEZE. Specify the 6 markers INV16, NPM1, INV16, NPM1, FLT3, Thu/ Mon PCR, selected on Test Request Form. FLT3, MLLAF9, MLL-AF9, MLL-AF4, QUALITATIVE MLL-AF4, MLL- MLL-ENL, t(12;21), ENL, t(12;21), t(1;19). t(1;19).

PAGE 280 OF 399 *Microscopy of Bone Marrow Aspirate & Peripheral Blood *Special Stains *CBC * 4 mL (2.5 mL min.) Bone Marrow Aspirate in 2 Histopathology Green Top ( Sodium Heparin) tubes AND 4 Bone *Leukemia Marrow Aspirate Smears. Air dry & fix smears in Flow Cytometry, LEUKEMIA diagnostic panel, 100% Methanol for 30 minutes AND 3 mL (2 mL Culture, Sample Daily by DIAGNOSTIC Acute Leukemia - min.) Whole blood / Bone Marrow in 1 Lavender Microscopy, Z536 11 am; Report 7 COMPREHENSIV T, B or Myeloid Top (EDTA) tube AND 2 Peripheral blood smears Karyotype, Real days E PROFILE 1 *Chromosome AND Bone Marrow Trephine biopsy in 10% formal Time PCR, HPE, analysis for salin. Ship at specified temperatures. Give clinical Electrical Impedence hematological history on Bone Marrow & ChromosomeTest malignancy Request Forms. *AML categorization by PCR / BCR ABL gene rearrangeÇ

PAGE 281 OF 399 *Microscopy of Bone Marrow Aspirate & Peripheral Blood *Special Stains *CBC 4 mL (2.5 mL min.) Bone Marrow Aspirate in 2 *Leukemiadiagno Green Top ( Sodium Heparin) tubes AND 4 Bone stic panel, Acute Marrow Aspirate Smears. Air dry & fix smears in Flow Cytometry, LEUKEMIA Leukemia - T, B 100% Methanol for 30 minutes AND 3 mL (2 mL Culture, Sample Daily by DIAGNOSTIC orMyeloid, min.) Whole blood / Bone Marrow in 1 Lavender Microscopy, Z537 11 am; Report 7 COMPREHENSIV *Chromosome Top (EDTA) tube AND 2 Peripheral blood smears Karyotype, Real days E PROFILE 2 analysis for AND Bone Marrow Trephine biopsy in 10% formal Time PCR, Electrical hematological saline. Ship at specified temperatures. Give clinical Impedence malignancy, history on Bone Marrow & ChromosomeTest *AML Request Forms. categorization by PCR / BCR ABL gene rearrangement, PCR QualitaÇ

*Chromosome 2 mL (1.5 mL min.) Bone Marrow Aspirate / Whole LEUKEMIA analysis for Blood in 1 Green Top (Sodium Heparin) tube AND 3 Culture, Sample Daily by DIAGNOSTIC hematologicalma mL (2 mL min.) Whole blood / Bone Marrow Microscopy, Z664 11 am; Report 7 COMPREHENSIV lignancy *AML Aspirate in 1 Lavender Top (EDTA) tube. Ship Karyotype, Real days E PROFILE 5 categorization refrigerated. DO NOT FREEZE. Give clinical history Time PCR by PCR on Chromosome Test Request Form.

PAGE 282 OF 399 *Leukemia diagnostic panel, Acute Leukemia - T, B or Myeloid 4 mL (3 mL min.) Bone Marrow Aspirate / Whole Flow Cytometry, LEUKEMIA *Chromosome Blood in 1 Green Top (Sodium Heparin) tube AND 3 Culture, Sample Daily by DIAGNOSTIC analysis for mL (2 mL min.) Whole blood / Bone Marrow Z663 Microscopy, 11 am; Report 7 COMPREHENSIV hematological Aspirate in 1 Lavender Top (EDTA) tube. Ship Karyotype, Real days E PROFILE 7 malignancy * refrigerated. DO NOT FREEZE. Give clinical history Time PCR Leukemia on Chromosome Test Request Form. Genetic Profile - any 6 markers, PCR

3 mL (2 mL min.) whole blood each in 1 Lavender LEUKEMIA Top (EDTA) tube AND 1 Green Top (Sodium Heparin) DIAGNOSTIC tube OR 2 mL (1 mL min.) heparinized Bone Marrow Sample Daily by 9 Z268 PANEL:ACUTE AND Aspirate Smear. Ship immediately at 18–22°C. Flow Cytometry am; Report Same LEUKEMIA - T, DO NOT REFRIGERATE OR FREEZE. Specify time, Day B or MYELOID date and clinical details on test request form. Clinical History Mandatory

3 mL (2 mL min.) whole blood each in 1 Lavender LEUKEMIA Top (EDTA) tube AND 1 Green Top (Sodium Heparin) DIAGNOSTIC tube OR 2 mL (1 mL min.) heparinized Bone Marrow Sample Daily by 9 Z269 PANEL:AML AND Aspirate Smear. Ship immediately at 18–22°C. Flow Cytometry am; Report Same CHARACTERIZAT DO NOT REFRIGERATE OR FREEZE. Specify time, Day ION date and clinical details on test request form. Clinical History Mandatory.

PAGE 283 OF 399 3 mL (2 mL min.) whole blood each in 1 Lavender Top (EDTA) tube AND 1 Green Top (Sodium Heparin) LEUKEMIA tube OR 2 mL (1 mL min.) heparinized Bone Marrow Sample Daily by 9 MONITORING Z154 AND Aspirate Smear. Ship immediately at 18–22°C. Flow Cytometry am; Report Same PANEL: HAIRY DO NOT REFRIGERATE OR FREEZE. Specify time, Day CELL date and clinical details on test request form. Clinical History Mandatory.

3 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th of LEVETIRACETA Additive) tube. Ship refrigerated or frozen. the month; RW205 LC-MS/MS M Recommended specimen: Just before next Report after 2–3 scheduled dose. Do not use SST gel barrier tubes. weeks

In females, LH; 2 mL (1 mL min.) serum from 1 SST. Ship preferred sampling Chemiluminescent R081 LUTEINISING refrigerated or frozen. Specify age and sex on test time is Day 2 / Day 3 Daily Immunoassay HORMONE request form. of the menstrual cycle.

In females, LH & 3 mL (1 mL min.) serum from 1 SST. Ship preferred sampling Chemiluminescent Z207 TESTOSTERONE, refrigerated or frozen. Specify age and sex on test time is Day 2 / Day 3 Daily Immunoassay TOTAL request form. of the menstrual cycle. 2 mL (1 mL min.) serum from 1 SST. Ship B059 LIPASE Spectrophotometry Daily refrigerated or frozen. LIPID - Sample by 7th of ASSOCIATED 2 mL (1 mL min.) serum from 1 Red Top (No the month; RW122 Colorimetry SIALIC ACID; Additive) tube. Ship refrigerated or frozen. Reportafter 2–3 LASA weeks

PAGE 284 OF 399 *Cholesterol *Triglycerides 3 mL (1.5 mL min.) serum from 1 SST. Ship *HDL, Direct LIPID PROFILE, refrigerated or frozen. Minimum 12 hours fasting is B131 *LDL,Direct Spectrophotometry, Daily BASIC mandatory. Grossly hemolysed samples are *VLDL, notacceptable. Calculated*Non HDL Cholesterol

*Apolipoproteins A1, B & Ratio *Lipoprotein (a) *Cholesterol *Triglycerides 3 mL (2 mL min.) serum from 1 SST. Ship *HDL/VLDL/LDLEl Spectrophotometry, LIPID PROFILE, refrigerated or frozen. Minimum 12 hours fasting is Sample Daily by 9 Z060 ectrophoretic Electrophoresis,Imm EXTENDED mandatory. Grossly hemolysed samples are not am; Report 3 days *Cholesterol / unoturbidimetry acceptable. HDL Ratio*Chylomicr ons *Non HDL Cholesterol * Cardio CRP

PAGE 285 OF 399 *Cholesterol *Triglycerides *HDL/VLDL/LDL - 3 mL (2 mL min.) serum from 1 SST. Ship Electrophoretic LIPID PROFILE, refrigerated or frozen. Minimum 12 hours fasting is Spectrophotometry, Sample Daily by 9 E017 *Cholesterol / COMPLETE mandatory. Grossly hemolysed samples are not Electrophoresis, am; Report 3 days HDL acceptable. Ratio*Chylomicr ons*Non HDL Cholesterol

*Apolipoproteins 4 mL (2 mL min.) serum from 2 SST's. Ship A1, B & Ratio refrigerated. DO NOT FREEZE AND 2 mL (1.5 mL *Lipoprotein (a) min.) Platelet Poor Plasma (PPP) from 1 Blue Top *Cholesterol (Sodium Citrate) tube. Prepare PPP within 1 hour of *Triglycerides collection as follows: Centrifuge sample at 3600 Spectrophotometry, *LDL Cholesterol rpm for 15 min & transfer supernatent to a clean Sample Mon LIPID PROFILE, CMIA , *HDL plastic tube. Centrifuge this supernatant again at through Sat by 4 Z279 COMPREHENSIV Imunoturbidimetry Cholesterol 3600 rpm for 15 mins & finally transfer the pm; Report Same E & Photo optical clot *Cholesterol / supernatant (PPP) to 1 labelled, clean plastic screw day detection HDL Ratio *Non capped vial. FREEZE IMMEDIATELY. Ship frozen. DO HDL Cholesterol NOT THAW. Duly filled Coagulation Requisition *Homocysteine Form is mandatory. Grossly hemolysed samples are *Fibrinogen not acceptable. Minimum 12 hours fasting is *Cardio CRP mandatory.

LIPOPROTEIN Sample by 7th of FRACTIONATIO 4 mL (2 mL min.) serum from 2 Red Top (No Ultracentrifugation, Also see LIPID the month; RW123 N, Additive) tubes. Ship refrigerated or frozen. Colorimetric,Enzyma PROFILE TESTS. Reportafter 2–3 ULTRACENTRIF Minimum 12hours fasting is mandatory. tic weeks UGATION

PAGE 286 OF 399 LIPOPROTEIN 2 mL (1 mL min.) serum from 1 SST. Ship B101 Immunoturbidimetry Daily (a); Lp(a) refrigerated or frozen. LISTERIA Sample Daily 1 5 g fresh STOOL in a sterile screw capped container. M203 ANTIGEN, ICT pmReport Same Ship refrigerated only. DO NOT FREEZE. STOOL day 2 mL (1 mL min.) serum from 1 SST. Ship Ion Selective B069 LITHIUM refrigerated or frozen. Recommended specimen: Daily Electrode just before the next dose is due at steady state.

*SGOT *SGPT *GGTP *Bilirubin LIVER PANEL 1; 2 mL (1.5 mL min.) serum from 1 SST. Ship Z005 *Protein,Total Spectrophotometry Daily LFT refrigerated or frozen. *Alkaline Phosphatase

It is recommended 3 mL (2 mL min.) serum from 1 SST AND 3 mL that patient Whole blood in 1 Blue Top (Sodium Citrate) tube. discontinues *SGOT *SGPT Mix thoroughly by inversion. Transport to Lab Heparin for 1 day *GGTP *Bilirubin within 4 hours.. If this is not possible, make PPP and Oral *Protein, Total * within 1 hour of collection as follows: Centrifuge Spectrophotometry, Anticoagulants for 7 Alkaline sample at 3600 rpm for 15 min & transfer ElectromechanicalCl Sample Daily by 9 LIVER FIBROSIS days prior to Z618 Phosphatase * supernatent to a clean plastic tube. Centrifuge this ot Detection, am; Report Same PANEL sampling as these ProthrombinTim supernatant again at 3600 rpm for 15 mins & finally Capillary Day drugs may affect e * Protein transfer the supernatant (PPP) to 1 labelled, clean Electrophoresis test results. Electrophoresis, plastic screw capped vial. FREEZE IMMEDIATELY. Discontinuation Serum Ship frozen. DO NOT THAW. Overnight fasting is should be with prior preferred. Duly filled Coagulation Requisition Form consent from the is mandatory. treating Physician.

PAGE 287 OF 399 It is recommended 3 mL ( 2 mL min) serum from 1 SST AND 3 mL Whole that patient blood in 1 Blue Top (Sodium Citrate) tube. Mix discontinues thoroughly by inversion. Transport to Lab within 4 Heparin for 1 day *SGOT *SGPT hrs. If this is not possible, make PPP within 1 hour of and Oral *GGTP *Bilirubin collection as follows: Centrifuge sample at 3600 Anticoagulants for 7 *Protein,Total Spectrophotometry, rpm for 15 min & transfer supernatent to a clean days prior to Z004 LIVER PANEL 2 *Alkaline CMIA,Electromechan Daily plastic tube. Centrifuge this supernatant again at sampling as these Phosphatase ical Clot Detection 3600 rpm for 15 mins & finally transfer the drugs may affect *Prothrombin supernatant (PPP) to 1 labelled, clean plastic screw test results. Time *HBsAg capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician.

LIVER KIDNEY MICROSOMAL Sample Daily by 3 mL (1.5 mL min.) serum from 1 SST. Ship Immunofluorescence S165 (LKM) 11 am; Report refrigerated or frozen. Assay ANTIBODY IN Nextday DILUTIONS, IFA

LIVER KIDNEY For SERIAL Sample Daily by MICROSOMAL 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence S164 DILUTIONS, use 11 am; Report (LKM) refrigerated or frozen. Assay testcode S165. Same Day ANTIBODY, IFA 3 mL (1.5 mL min.) serum from 1 SST. Avoid use of LIVER & KIDNEY tourniquet and fist clenching during sampling. Spectrophotometry, Z025 *LFT * KFT Daily PANEL Overnight fasting is preferred. Ship refrigerated or Indirect ISE frozen.

PAGE 288 OF 399 Lp-PLA2 (LIPOPROTEIN Sample by Tue / 2 mL (1 mL min.) serum from 1 SST. Ship B192 ASSOCIATED Enzymatic Fri 9 am ; Report refrigerated or frozen. PHOSPHOLIPAS Same day E A2), ACTIVITY 20 mL (10 mL min.) aliquot of random urine in a Sample by 7th of LSD, URINE, sterile screw capped container. Ship refrigerated or the month; RW124 GC / MS QUANTITATIVE frozen.Wrap container in aluminium foil to protect Report after 2–3 from light. weeks LUNG CANCER Submit formalin fixed paraffin embedded tissue Sample by Tue 11 Z789 MUTATION *ALK-1 *EGFR block OR three 4 micron sections on Poly-L-Lysine FISH, PCR am; Report Mon PANEL coated slides. Ship at room temperature.

It is recommended that patient discontinues 3 mL Whole blood in 1 Blue Top (Sodium Citrate) Heparin for 1 day tube. Mix thoroughly by inversion. Transport to Lab and Oral within 4 hrs. If this is not possible, make PPP within Anticoagulants for 7 1 hour of collection as follows: Centrifuge sample at days prior to LUPUS 3600 rpm for 15 min & transfer supernatent to a sampling as these Sample Daily by 3 ANTICOAGULAN *APTT *PTT - LA Electromechanical Z168 clean plastic tube. Centrifuge this supernatant again drugs may affect pm; Report Next T PANEL; LAC *dRVVT Clot Detection at 3600 rpm for 15 mins & finally transfer the test results. day PANEL supernatant (PPP) to 1 labelled, clean plastic screw Discontinuation capped vial. FREEZE IMMEDIATELY. Ship frozen. DO should be with prior NOT THAW. Overnight fasting is preferred. Duly consent from the filled Coagulation Requisition Form is mandatory. treating Physician. Also see PHOSPHOLIPID SYNDÇ

PAGE 289 OF 399 It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium Citrate) discontinues tube. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4hrs. If this is not possible, make PPP within 1 and Oral hour of collection as follows: Centrifuge sample at Anticoagulants for 7 LUPUS 3600 rpm for 15 min & transfer supernatent to a Sample Daily by 3 Electromechanical days prior to H123 ANTICOAGULAN clean plastic tube. Centrifuge this supernatant again pm; Report Next Clot Detection sampling as these T BY dRVVT at 3600 rpm for 15 mins & finally transfer the day drugs may affect supernatant (PPP) to 1 labelled, clean plastic screw test results. capped vial. FREEZE MMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory consent from the treating Physician.

It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium Citrate) discontinues tube. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral 1 hour of collection as follows: Centrifuge sample at LUPUS Anticoagulants for 7 3600 rpm for 15 min & transfer supernatent to a Sample Daily by 3 ANTICOAGULAN Electromechanical days prior to H124 clean plastic tube. Centrifuge this supernatant again pm; Report Next T BY APTT; PTT - Clot Detection sampling as these at 3600 rpm for 15 mins & finally transfer the day LA drugs may affect supernatant (PPP) to 1 labelled, clean plastic screw test results. capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician.

PAGE 290 OF 399 LYME DISEASE (BORRELIA Sample by Sat 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S092 BURGDORFERI) am; Report Same refrigerated or frozen. Immunoassay ANTIBODIES, day IgG & IgM

LYME DISEASE (BORRELIA Sample by 7th of BURGDORFERI) 2 mL (1 mL min.) CSF / Synovial Fluid in a sterile the month; RW024 Real Time PCR DNA, screw capped container. Ship refrigerated or frozen. Reportafter 2–3 QUALITATIVE weeks REAL TIME PCR LYME DISEASE Sample by 7th of (BORRELIA 2 mL (1 mL min.) serum from 1 SST. Ship the month; RW125 BURGDORFERI), Immunoblot refrigerated or frozen. Reportafter 2–3 IgG & IgM, weeks IMMUNOBLOT LYME DISEASE Sample by 7th of (BORRELIA 2 mL (1 mL min.) serum from 1 SST. Ship the month; RW126 BURGDORFERI), Immunoblot refrigerated or frozen. Reportafter 2–3 IgG, weeks IMMUNOBLOT Blood: 3 mL (2 mL min.) whole blood from 1 Lavender Top (EDTA) tube.Bone Marrow: 3 mL (1.5 mL min.) in 1 Green Top (Sodium Heparin) LYMPHOMA Sample Daily by 9 tube.Body Fluids: 50 mL (10 mL min.) fluids in a Z149 DIAGNOSTIC Flow Cytometry am; Report Same sterile screw capped container.Lymph Node PANEL Day Aspirate: 1 mL (0.5 mL min.) in normal saline. Ship at 18–22°C. DO NOT REFRIGERATE OR FREEZE. Clinical history is mandatory.

PAGE 291 OF 399 Sample by 7th of 2 mL (1 mL min.) serum from 1 SST. Separate serum LYSOZYME; the month; RW127 within 1 hour of draw in a plastic transport vial. Enzymatic MURAMIDASE Reportafter 2–3 Shiprefrigerated or frozen. weeks 3 mL (2 mL min.) serum from 1 Red Top (No MACROCYTIC *Folate, serum * Additive) tube. Wrap tube in aluminium foil to Chemiluminescent Z125 Daily ANEMIA PANEL Vitamin B12 protect from light. Ship refrigerated or frozen. Immunoassay Overnight fasting is mandatory. Sample by 7th of MACROPROLAC 2 mL (1 mL min.) serum from 1 SST. Ship the month; RW249 PEG, ECLIA TIN refrigerated or frozen. Report after 2–3 weeks 10 mL (5 mL min.) aliquot of 24-hour urine in a MAGNESIUM, sterile screw capped container. Do not use U019 24-HOUR preservatives. Record 24-hour volume on test Xylidyl blue Daily URINE request form and urine container. Ship refrigerated or frozen. MAGNESIUM, 2 mL (1 mL min.) serum from 1 SST. Ship B066 Xylidyl blue Daily SERUM refrigerated or frozen. MAGNESIUM, 10 mL (5 mL min.) aliquot of random urine in a U060 RANDOM sterile screw capped container. Do not use Xylidyl blue Daily URINE preservatives. Ship refrigerated or frozen. MALARIA PARASITE / Submit two thin AND two thick peripheral blood Microscopic H030 BLOOD Daily smears. Examination PARASITE IDENTIFICATION MALARIA VIVAX AND 3 mL (2 mL min.) whole blood from 1 Lavender Top Immunochromatogr S153 Daily FALCIPARUM (EDTA) tube. Ship refrigerated. DO NOT FREEZE. aphy ANTIGEN

PAGE 292 OF 399 Sample by Tue / MALARIA 2 mL (1 mL min.) serum from 1 SST. Ship Immunochromatogr S189 Fri 9 am; Report ANTIBODY refrigerated or frozen. aphy Same day

MALARIA Sample by Tue / 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS160 DETECTION , PCR Sat 11 am; (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. PCR Report Sat/Wed

MALARIA 3 mL (2 mL min.) whole blood from 1 Lavender Top H249 PARASITE Digital Cytometry Daily (EDTA) tube. Ship refrigerated. DO NOT FREEZE. CONFIRMATION MALDITOF-MS (Matrix assisted MALDITOF - MS Submit pure growth of Mycobacterium species on a laser desorption Sample by 9 am; M184 MYCOBACTERIU solid medium - LJ or Middlebrook agar. Ship ionisation Time of Report Same day M SPECIATION refrigerated. DO NOT FREEZE flight- Mass spectrometry) MALDITOF-MS (Matrix assisted MALDITOF-MS Submit pure growth of bacteria on a solid agar laser desorption Sample by 9 am; M189 BACTERIA plate. Ship refrigerated. DO NOT FREEZE. ionisation Time of Report Same day IDENTIFICATION flight- Mass spectrometry) MALDITOF-MS (Matrix assisted MALDITOF-MS Submit pure growth of mold on a solid agar plate. laser desorption Sample by 9 am; M190 MOLD Ship refrigerated. DO NOT FREEZE. ionisation Time of Report Same day IDENTIFICATION flight- Mass spectrometry)

PAGE 293 OF 399 MALDITOF-MS (Matrix assisted MALDITOF-MS Submit pure growth of yeast on a solid agar plate . laser desorption Sample by 9 am; M188 YEAST Ship refrigerated. DO NOT FREEZE. ionisation Time of Report Same day IDENTIFICATION flight- Mass spectrometry)

Use only specified 10 mL (5 mL min.) aliquot of 24-hour urine collected containers available in an acid washed (metal free) container kit from LPL. If available from LPL. Mix thoroughly. Transfer 10 mL gadolinium or iodine MANGANESE, aliquot to vial provided in the kit. Measure 24 hour Sample Daily by 6 containing contrast C058 24-HOUR total volume and record on vial and test request ICPMS pm; Report Next media has been URINE form. Ship refrigerated or frozen. Patients should day administered, the refrain from vitamins, mineral or herbal specimen should not supplements one week prior to specimen collection. be collected for 96 Use powderless gloves during specimen collection. hours.

Use only specified containers available 4 mL (2 mL min.) whole blood from 1 metal free from LPL. If Royal Blue Top (K2EDTA) tube available from LPL. gadolinium or iodine Sample Daily by 6 MANGANESE, Ship refrigerated. DO NOT FREEZE. Patients should containing contrast C064 ICPMS pm; Report Next BLOOD refrain from vitamins, mineral or herbal media has been Day supplements one week prior to specimen collection. administered, the Use powderless gloves during specimen collection. specimen should not be collected for 96 hours.

PAGE 294 OF 399 Use only specified containers available 10 mL (5 mL min.) aliquot of random urine collected from LPL. If in an acid washed (metal free) container available gadolinium or iodine MANGANESE, Sample Daily by 6 from LPL. Ship refrigerated or frozen. Patients containing contrast C059 RANDOM ICPMS pm; Report next should refrain from vitamins, mineral or herbal media has been URINE day supplements one week prior to specimen collection. administered, the Use powderless gloves during specimen collection. specimen should not be collected for 96 hours.

MANTOUX TEST; Patient to report to NRL / Satellite Labs on Day 1 48 hrs post- P007 Intradermal skin test TUBERCULIN and Day 3 of the test. injection SKIN TEST 10 mL Whole blood in a special tube available from *Trisomy MATERNAL LPL. Ship refrigerated. DO NOT FREEZE. Valid Sample by Tue 9 detection for 13, N075 BLOOD FOR between 10-24 weeks of gestation. Give clinical DNA Sequencing am; Report next 18 & 21 FETAL DNA history on Maternal Serum Screen request form Tue Chromosomes available from LPL. 3 mL (1.5 mL min.) serum from 1 SST. Ship refrigerated or frozen. Provide maternal Date of birth (dd/mm/yy); LMP or Ultrasound; IVF, Number Sample Mon MATERNAL *AFP *BETA HCG of Fetuses (Single/ Twins); Diabetic status and Body Chemiluminescent through Sat by 9 Z266 SERUM SCREEN *Free Estriol Weight in Kg, Smoking &Previous history of Trisomy Immunoassay am; Report Same 3; TRIPLE TEST *Risk Evaluation 21 pregnancy in Maternal Serum Screen Form. day Valid between 14-22 weeks gestation (Ideal 15-20 weeks).

PAGE 295 OF 399 3 mL (1.5 mL min.) serum from 1 SST. Ship refrigerated or frozen. Provide maternal Date of MATERNAL *AFP *BETA HCG birth (dd/mm/yy); LMP or Ultrasound; IVF, Number Sample Mon SERUM SCREEN *Free Estriol of Fetuses (Single/ Twins); Diabetic status and Body Chemiluminescent through Sat by 9 Z514 4; QUADRUPLE *Inhibin A *Risk Weight in Kg, Smoking & Previous history of Trisomy Immunoassay am; Report Same TEST Evaluation. 21 pregnancy in Maternal Serum Screen Form. day Valid between 14-22 weeks gestation (Ideal 15-20 weeks). For Combined Risk 3 mL (1.5 mL min.) serum from 1 SST. Ship Assessment refrigerated or frozen. Provide maternal Date of (Biochemical risk + birth (dd/mm/yy); LMP or Ultrasound; Number of Nuchal translucency) Sample Mon MATERNAL *PAPP-A *Free Fetuses (Single/Twins); Diabetic status and Body Chemiluminescent kindly enclose through Sat by 9 Z397 SERUM SCREEN BETA HCG *Risk Weight in Kg, IVF, Smoking & Previous history of Immunoassay Ultrasound report am; Report Same 2; DUAL TEST Evaluation. Trisomy 21 pregnancy in Maternal Serum Screen between 11-13 day Form. Valid between 9-13 weeks gestation (Ideal 10- weeks gestation 13 weeks). including CRL, NT & Nasal Bone.

MEAN 3 mL (1 mL min.) whole blood in 1 Lavender Top H005 CORPUSCULAR Calculated Daily (EDTA) tube. Ship refrigerated. DO NOT FREEZE. VOLUME; MCV

MEAN CORPUSCULAR 3 mL (1 mL min.) whole blood in 1 Lavender Top H007 HEMOGLOBIN Calculated Daily (EDTA) tube. Ship refrigerated. DO NOT FREEZE. CONCENTRATIO N; MCHC

MEAN Electronic Cell CORPUSCULAR 3 mL (1 mL min.) whole blood in 1 Lavender Top H006 Cytometry, Daily HEMOGLOBIN; (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Calculated MCH

PAGE 296 OF 399 MEAN 3 mL (1 mL min.) whole blood in 1 Lavender Top H033 PLATELET Electrical Impedence Daily (EDTA) tube. Ship refrigerated. DO NOT FREEZE. VOLUME; MPV MEASLES (RUBEOLA) Sample by Thu 9 2 mL (1 mL min.) serum from 1 SST.Ship refrigerated Enzyme Z191 ANTIBODIES am; Report Same or frozen. Immunoassay PANEL, IgG & day IgM MEASLES Sample by Thu 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S072 (RUBEOLA) am; Report Same refrigerated or frozen. Immunoassay ANTIBODY, IgG day

MEASLES Sample by Thu 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S073 (RUBEOLA) am; Report Same refrigerated or frozen. Immunoassay ANTIBODY, IgM day MEDULLARY THYROID Sample Daily by 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS161 CARCINOMA, PCR 11 am; Report (EDTA) tube. Ship refrigerated. DO NOT FREEZE. MUTATION 10 days DETECTION

MELAS (MITOCHONDRI AL ENCEPHALOPAT Sample Daily by HY & LACTIC 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS162 PCR 11 am; Report 10 ACIDOSIS WITH (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. days STROKE LIKE SYNDROME), MUTATION DETECTION

PAGE 297 OF 399 It is recommended that patient 6 mL Whole blood in 2 Blue Top (Sodium Citrate) discontinues tubes. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral *Prothrombin 1 hour of collection as follows: Centrifuge sample at Anticoagulants for 7 Time *APTT 3600 rpm for 15 min & transfer supernatent to a Electromechanical & Sample by Thu MENNORHAGIA days prior to H244 *Factor VIII clean plastic tube. Centrifuge this supernatant Photo-optical 11 am; Report SCREEN sampling as these Functional again at 3600 rpm for 15 mins & finally transfer the ClotDetection Next day. drugs may affect *Factor IX supernatant (PPP) to 1 labelled, clean plastic screw test results. capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred. Duly should be with prior filled coagulation Requisition form is mandatory. consent from the treating Physician.

*Estradiol (E2) *TSH, MENOPAUSAL Sample Daily by 9 Ultrasensitive * 5 mL ( 3 mL min.) serum from 1 SST. Ship Z808 SCREENING CLIA, EIA am; Report Same FSH *Anti refrigerated or frozen. PANEL day Mullerian hormone (AMH)

PAGE 298 OF 399 Use only specified 10 mL (5 mL min.) aliquot of 24 hr urine collected in containers availabe an acid washed (metal free) container kit available from LPL. If from LPL. Mix thoroughly. Transfer 10 mL aliquot to gadolinium or iodine vial provided in the kit. Measure 24 hour total Sample Daily by 6 MERCURY, 24- containing contrast C028 volume and record on vial and test request form. ICPMS pm; Report Next HOUR URINE media has been Ship refrigerated or frozen. Patient should refrain day administered, the from eating predatory fish like Sword Fish, Tuna and specimen should not Shark at least 3 days prior to specimen collection. be collected for 96 Use powderless gloves during specimen collection. hours.

Use only specified container available 10 mL (5 mL min.) aliquot of random urine collected from LPL. If in an acid washed (metal free) container available gadolinium or iodine MERCURY, from LPL. Ship refrigerated or frozen. Patient should Sample Daily by 6 containing contrast C029 RANDOM refain from eating predatory fish like Sword fish, ICPMS pm; Report next media has been URINE Tuna and shark atleast 3 days prior to specimen day administered, the collection. Use powderless gloves during specimen specimen should not collection. be collected for 96 hours.

MERRF (MYOCLONIC EPILEPSY Sample Daily by ASSOCIATED 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS163 PCR 11 am; Report WITH RAGGED (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. 10 days RED FIBRES), MUTATION DETECTION

PAGE 299 OF 399 *Reducing Substances *Ferric Chloride 10 ml (5 ml min) aliquot of first morning urine in a Test*DNPH Test METABOLIC sterile screw capped container. No preservative *Nitrosonaphthol Sample by Mon / SCREEN, required. Ship refrigerated or frozen. For infants on Chemical, Thin Layer G108 Test Wed / Fri 4 pm; COMPREHENSIV breast milk, feed the child and then collect urine. Chromatography *Nitroprusside Report 2 days E, URINE Provide a brief clinical and drug history over the last Test *Silver 3 days prior to specimen collection. nitroprusside Test *Amino Acids, Qualitative

*Reducing Substances *Ferric Chloride 10 ml (5 ml min) aliquot of first morning urine in a Test *DNPH Test sterile screw capped container. Do not use any METABOLIC Sample Daily by 4 *Nitrosonaphthol preservatives. Ship refrigerated or frozen. For G101 SCREEN, BASIC, Chemical pm; Report Same Test infants on breast milk, feed the child and then URINE day *Nitroprusside collect urine. Provide a brief clinical and drug history Test * Silver over the last 3 days prior to specimen collection. nitroprusside Test METACHROMA 10 mL (7.5 mL min.) whole blood from3 Lavender TIC Top (EDTA) tubes OR 10 mL (7.5 mL min.) whole This tests deficiency Sample Daily by 4 LEUCODYSTROP G122 blood from 3 Green Top (Sodium Heparin) tubes. Spectrophotometry of enzyme Aryl pm; Report 4 HY, Ship refrigerated. DO NOT FREEZE. Provide brief Sulphatase A. days QUANTITATIVE, clinical history. BLOOD

PAGE 300 OF 399 Use only specified containers available 4 mL ( 2 mL min.) whole blood from 1 metal free *Arsenic * from LPL. If Royal Blue Top (K2EDTA) tube available from LPL. Cadmium gadolinium or iodine METAL SCREEN Patient should avoid eating seafood, herbal Sample Daily by 6 *Chromium ICPMS, AAS with containing contrast Z820 2, WHOLE supplements, mineral supplements, vitamin B12 or pm; Report Next *Cobalt *Lead Zeeman correction media has been BLOOD vitamin B complex for 3 days prior to specimen day *Manganese administered, the collection. Ship refrigerated. DO NOT FREEZE. Use *Thalium specimen should not powderless gloves during specimen collection. be collected for 96 hours.

10 mL (5 mL min.) aliquot of 24 hour urine collected Use only specified *Arsenic * in an acid washed (metal free) container kit containers available Cadmium available from LPL. Mix thoroughly. Transfer 10 mL from LPL. If *Chromium aliquot to vial provided in kit. Measure 24 hour total gadolinium or iodine *Cobalt *Lead volume and record on vial and Test request form. Sample Daily by 6 METAL SCREEN AAS withZeeman containing contrast Z821 *Manganese Patient should avoid eating seafood, herbal pm; Report Next 3, URINE correction, ICPMS media has been *Thalium * supplements, mineral supplements, vitamin B12 or day administered, the Copper * Zinc vitamin B complex, sea/fresh water fish for atleast 3 specimen should not *Selenium days prior to specimen collection.Ship refrigerated be collected for 96 *Nickel *Mercury or frozen. Use powderless gloves during specimen hours. collection.

PAGE 301 OF 399 Use only specified containers available 3 mL ( 1 mL min.) serum from 1 metal free White from LPL. If Top (Z-No Additive) tube available from LPL. gadolinium or iodine *Aluminum * Patients should refrain from taking antacids, Sample Daily by 6 METAL SCREEN containing contrast Z822 Copper * Zinc Aluminium containing medications, sea/fresh water ICPMS pm; Report Next 1, SERUM media has been *Selenium fish atleast 3 days prior to specimen collection.Ship day administered, the refrigerated. DO NOT FREEZE. Use powderless specimen should not gloves during specimen collection. be collected for 96 hours.

50 mL (10 mL min.) aliquot of 24-hour urine. Collect urine with appropriate volume of 50% HCl to maintain pH between 1–2. Do not use concentrated HCl. Record 24 hour volume on test request form and urine container. Ship refrigerated or frozen. METANEPHRINE *Metanephrine High Performance Also see Patient should strictly avoid Alpha- Methyldopa, Sample by Fri 9 U035 S, 24-HOUR *Normetanephri LiquidChromatograp PHEOCHROMOCYTO Buspirone, Codeine, Isoproterenol metabolite, am; Report Tue URINE ne hy MA PROFILE Mandelamine, L-dopa, Parcetamol, Metoclopramide, pepper, alcoholic beverages, tea/coffee, tobacco and strenuous exercise at least 72 hours before and during specimen collection.

PAGE 302 OF 399 50 mL (10 mL min.) aliquot of random urine. Add 50% HCl drop by drop to bring the pH between 1–2. Do not use concentrated HCl. Ship refrigerated or frozen. Patient should strictly avoid Alpha- METANEPHRINE *Metanephrine High Performance Also see Sample by Fri 9 Methyldopa, Buspirone, Codeine, Isoproterenol U052 S, RANDOM *Normetanephri LiquidChromatograp PHEOCHROMOCYTO am; Report on metabolite, Mandelamine, L-dopa, Paracetamol, URINE ne hy MA PROFILE Tue Metoclopramide, pepper, alcoholic beverages, tea/coffee, tobacco and strenuous exercise at least 72 hours before and during specimen collection.

METHAMPHETA 10 mL (5 mL min.) aliquot of random urine in a Sample Daily by 4 Lateral Flow K019 MINE SCREEN, sterile screw capped container. Do not use pm; Report Same Immunoassay URINE preservatives. Ship refrigerated or frozen day

METHAQUALON 10 mL (5 mL min.) aliquot of random urine in a Sample Daily by 4 Lateral Flow K021 E SCREEN, sterile screw capped container. Do not use pm; Report Same Immunoassay URINE preservatives. Ship refrigerated or frozen. day

10 mL (5 mL min.) aliquot of random urine in a Sample Daily by 4 METHADONE Lateral Flow K022 sterile screw capped container. Do not use pm; Report Same SCREEN, URINE Immunoassay preservatives. Ship refrigerated or frozen. day Blood concentrations of 2 mL (1 mL min.) serum from 1 Red Top (No Methotrexate are Sample by Mon / METHOTREXAT Additive) tube. Do not use gel barrier tubes. Wrap monitored at 24, 48 D052 EMIT Wed / Fri 2 pm; E in Aluminium foil to protect from light. Ship & 72 hours aftera ReportSame day refrigerated or frozen. single high dose (>50 mg/m2) therapy.

PAGE 303 OF 399 METHYLGUANI Submit fresh tissue in normal saline in a sterile Sample Daily by NE OS164 screw capped container. Ship refrigerated . DO NOT PCR 11 am; Report METHYLTRANSF FREEZE 22 days ERASE (MGMT) METHYLMALON 2 mL (1 mL min.) serum from 1 SST. Separate serum Sample Daily by 4 IC ACID, G189 within 1 hour of collection. Ship refrigerated or LC-MS/MS pm; Report Next QUANTITATIVE, frozen. day SERUM METHYLMALON 10 ml (5 ml min.) aliquot of first morning urine in a Sample Daily by 4 IC ACID, sterile screw capped container. Do not use any G103 Chemical pm; Report Same QUALITATIVE, preservatives. Ship refrigerated or frozen. Provide a day URINE brief clinical and drug history.

METHYLMALON 10 ml (5 ml min.) aliquot of first morning urine in a Sample Daily by 4 IC ACID, sterile screw capped container. Do not use any G188 LC-MS/MS pm; Report Next QUANTITATIVE, preservatives. Ship refrigerated or frozen. Provide a day URINE brief clinical and drug history. MICM (MATERNALLY INHERITED Sample Daily by 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS165 CARDIOMYOPA PCR 11 am ; Report (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. THY), 10 days MUTATION DETECTION MICROALBUMI 15 mL (10 mL min.) aliquot of random urine in a Immunoturbidimetry B142 N CREATININE sterile screw capped container. Ship refrigerated. Daily , Spectrophotometry RATIO, URINE DO NOT FREEZE.

PAGE 304 OF 399 MICROALBUMI The recommended N, 1ST 10 ml (5 ml min.) aliquot of first morning / random test is U027 MORNING / urine in a sterile screw capped container. Ship Immunoturbidimetry Daily U031MICROALBUMI RANDOM refrigerated. DO NOT FREEZE. N, 24-HOUR URINE. URINE 10 mL (5 mL min.) aliquot of 24-hour urine in a MICROALBUMI sterile screw capped container. Do not use U031 N, 24-HOUR preservatives. Record 24 hour volume on test Immunoturbidimetry Daily URINE request form and urine container. Ship refrigerated. DO NOT FREEZE. 2 air dried peripheral blood smears (1 thick and 1 thin) OR 2 mL (1 mL min.) whole blood from 1 MICROFILARIA Lavender Top (EDTA) tube OR 2 mL (1 mL min.) Also see FILARIA H024 Microscopy Daily DETECTION Hydrocele fluid. Draw specimen between 12 ANTIGEN midnight to 2 a.m. Ship refrigerated. DO NOT FREEZE. Following *CD10 *CD13 requirements are *CD15 *CD19 3 mL (1 mL min.) First pull Bone Marrow aspirate in MINIMAL mandatory:• Time *CD20 *CD33 1 Green Top (Sodium Heparin) tube. Ship RESIDUAL of sampling• Sample Daily by 9 *CD34 *CD38 immediately at 18-22ͦC. DO NOT FREEZE. Following Z831 DISEASE (MRD) Flow Cytometry Previous am; Report Next *CD45 *CD58 requirements are mandatory:• Date & Time of ANALYSIS FOR immunophenotype day *CD73 *CD86 sampling• Previous immunophenotype report• B- ALL report• Clinical *CD123 *CD200 Clinical history history• Date & time *SYTO-16 of sampling MITOCHONDRI For SERIAL Sample Daily by 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence S046 AL ANTIBODY DILUTIONS, use 11 am; Report refrigerated or frozen. Overnight fasting is preferred. Assay (AMA), IFA testcode S085. Same Day

PAGE 305 OF 399 MITOCHONDRI Sample Daily by AL ANTIBODY 3 mL (1.5 mL min.) serum from 1 SST. Ship Immunofluorescence S085 11 am; Report (AMA), IFA IN refrigerated or frozen. Overnight fasting is preferred. Assay Nextday DILUTIONS

MITOCHONDRI *MALAS Sample Daily by AL MUTATION *MERRF *MM 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS166 PCR 11 am ; Report DETECTION *MMC *ME (EDTA) tube. Ship refrigerated. DO NOT FREEZE. 10 days PANEL 1 *PEM *EI

MITOCHONDRI *LHON *NARP Sample Daily by AL MUTATION 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS167 *CPEO *SNHL PCR 11 am ; Report DETECTION (EDTA) tube. Ship refrigerated. DO NOT FREEZE. *DEAF *AMDF 10 days PANEL 2

MITOCHONDRI AL MUTATION Sample Daily by 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS168 DETECTION PCR 11 am ; Report (EDTA) tube. Ship refrigerated. DO NOT FREEZE. COMPREHENSIV 10 days E PANEL

PAGE 306 OF 399 It is recommended that patient 6 mL Whole blood in 2 Blue Top (Sodium Citrate) discontinues tubes. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral MIXING *Prothrombin 1 hour of collection as follows: Centrifuge sample at Anticoagulants for 7 STUDIES - APTT Time *APTT 3600 rpm for 15 min & transfer supernatent to a Sample by Wed / Photo-optical Clot days prior to H242 WITH *Thrombin Time clean plastic tube. Centrifuge this supernatant again Sat 11 am; Detection sampling as these PROTHROMBIN *Fibrinogen * at 3600 rpm for 15 mins & finally transfer the ReportThu / Mon drugs may affect TIME STUDIES Mixing studies supernatant (PPP) to 1 labelled, clean plastic screw test results. capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred.Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician.

It is recommended that patient 6 mL Whole blood in 2 Blue Top (Sodium Citrate) discontinues tubes. Mix thoroughly by inversion. Transport to Lab Heparin for 1 day within 4 hrs. If this is not possible, make PPP within and Oral 1 hour of collection as follows: Centrifuge sample at *Fibrinogen Anticoagulants for 7 3600 rpm for 15 min & transfer supernatent to a Sample by Wed / MIXING *Thrombin Time Photo-optical Clot days prior to H184 clean plastic tube. Centrifuge this supernatant again Sat 11 am; STUDIES - APTT *APTT *Mixing Detection sampling as these at 3600 rpm for 15 mins & finally transfer the ReportNext day studies drugs may affect supernatant (PPP) to 1 labelled, clean plastic screw test results. capped vial. FREEZE IMMEDIATELY. Ship frozen. DO Discontinuation NOT THAW. Overnight fasting is preferred.Duly should be with prior filled Coagulation Requisition Form is mandatory. consent from the treating Physician.

PAGE 307 OF 399 MMC (MATERNAL MYOPATHY Sample Daily by WITH 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS169 PCR 11 am ; Report CARDIOMYOPA (EDTA) tube. Ship refrigerated. DO NOT FREEZE. 10 days THY), MUTATION DETECTION MM (MITOCHONDRI Sample Daily by AL 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS170 PCR 11 am ; Report MYOPATHY), (EDTA) tube. Ship refrigerated. DO NOT FREEZE. 10 days MUTATION DETECTION MODIFIED ACID Submit Slit -skin smear, Nasal Smear, Ear lobe Tissue Sample Daily by 9 M187 FAST STAIN smear and other secretions as applicable. Ship at Light Microscopy am; Report Same FOR LEPROSY 18–22°C. day MONO TEST (INFECTIOUS 2 mL (1 mL min.) serum from 1 SST. Ship frozen. DO Also see EPSTEIN- S004 Latex Agglutination Daily MONONUCLEOS NOT THAW BARR VIRUSTESTS. IS) MPL (MYELOPROLIFE Sample by Mon / RATIVE 3 ml (2 mL min.) whole blood from 1 Lavender Top PCR, Fragment N121 Thu 11 am; LEUKEMIA), (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Analysis Report Wed / Sat GENE MUTATION

PAGE 308 OF 399 MTHFR (METHYLENE TETRAHYDROFO LATE Sample by Mon / 3 mL (2 mL min.) Whole blood in 1 Lavender Top N049 REDUCTASE) Real Time PCR Thu 11 am; (EDTA) tube. Ship refrigerated. DO NOT FREEZE GENE ReportWed / Sat MUTATION, QUALITATIVE PCR MUCOPOLYSAC CHARIDOSIS 10 mL (7.5 mL min.) whole blood from 3 Lavender This tests deficiency Sample Daily by 4 (MPS) TYPE 1 Top (EDTA) tubes OR Green Top (Sodium Heparin) G112 Enzyme assay of enzyme Alpha pm; Report 4 (HURLER), tubes. Ship refrigerated. DO NOT FREEZE. Provide Iduronidase. days QUANTITATIVE, brief clinical history. BLOOD MUCOPOLYSAC CHARIDOSIS 10 mL (7.5 mL min.) whole blood from 3 Lavender (MPS) TYPE VI This tests deficiency Sample Daily by 4 Top (EDTA) OR Green Top (Sodium Heparin) tubes. G113 (MAROTEAUX Enzyme assay of enzyme Aryl- pm; Report 4 Ship refrigerated. DO NOT FREEZE. Provide brief LAMY), Sulphatase B. days clinical history. QUANTITATIVE, BLOOD MUCOPOLYSAC 10 ml (5 ml min.) aliquot of first morning urine in a Sample Daily by 4 CHARIDOSIS sterile screw capped container. Do not use any Toluidine blue spot G110 pm; Report Next (MPS) SCREEN, preservatives. Ship refrigerated or frozen. Provide test day URINE brief clinical and drug history.

MUCOPOLYSAC 20 mL (10 mL min.) aliquot of 24 hour urine in a Sample Daily by 4 CHARIDOSIS sterile screw capped container. Do not use any Two dimensional G111 pm; Report 15 (MPS) TYPING, preservatives. Ship refrigerated or frozen. Provide a electrophoresis days 24 HOUR URINE brief clinical and drug history.

PAGE 309 OF 399 Strictly adhere to specimen *IgG Synthesis volumes.Also see LPL: Sample Daily Index & Rate MYELIN ASSOCIATED by 9 am; Report BCG, Nephelometry, MULTIPLE *Oligoclonal 5 ml (3 ml min.) Cerebrospinal fluid AND 2 mL (1 mL GLYCOPROTEIN; Next dayUSA: Immunoturbidimetry Z182 SCLEROSIS Bands, CSF min.) serum from 1 SST. Ship refrigerated. DO NOT MYELIN ASSOCIATED Sample by 7th of , Agarose Gel PANEL 2 *Myelin Basic FREEZE GLYCOPROTEIN - the Month; Electrophoresis, RIA Protein, CSF SULFATED - Report after 2–3 (Code RW135) GLUCURONIC PARA- weeks. GLOBOSIDE AUTOANTIBODY, IgM

*IgG Synthesis 4 ml (3 ml min.) Cerebrospinal fluid AND 2 mL (1 mL BCG, Nephelometry, MULTIPLE Strictly adhere to Sample Daily by 9 Index & Rate min.) serum from 1 SST. Ship refrigerated. DO NOT Immunoturbidimetry Z181 SCLEROSIS specimen volumes am; Report Next *Oligoclonal FREEZE. The collection date and time must be the , Agarose Gel PANEL 1 for CSF day Bands, CSF same for both specimens. Electrophoresis

*Protein electrophoresis, serum * Immunofixation Capillary MULTIPLE electrophoresis, electrophoresis, Sample Daily by 9 MYELOMA 5 mL (3 mL min.) serum from 1 SST. Ship Z782 serum * Kappa / Agarose Gel am; Report Next SCREENING refrigerated or frozen. Lambda Light Electrophoresis, day PANEL Chains, Free, Immunoturbidimetry serum * Immunoglobulin Profile, serum

PAGE 310 OF 399 MUMPS VIRUS Sample by Mon 9 ANTIBODIES 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z382 am; Report Same PANEL, IgG & refrigerated or frozen. Immunoassay day IgM

Sample by Mon 9 MUMPS VIRUS 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S037 am; Report Same ANTIBODY, IgG refrigerated or frozen. Immunoassay day

Sample by Mon 9 MUMPS VIRUS 2 mL (1 mL min.) serum from 1 SST.Ship refrigerated Enzyme S075 am; Report Same ANTIBODY, IgM or frozen. Immunoassay day

MuSK (MUSCLE Sample by Fri 9 SPECIFIC 4 mL (2 mL min.) serum from 1 SST. Ship S243 Radioimmunoassay am; report Next KINASE) refrigerated or frozen. day ANTIBODY

*Acetylcholine Receptor Binding 5 mL (3 mL min.) serum from 2 SST’s.Ship Antibody Sample by Wed / MYASTHENIA refrigerated or frozen. Patient should not have Radioimmunoassay, Z174 *Striated / Sat 9 am; Report GRAVIS PANEL general anaesthetic or muscle relaxant drugs, 24 IFA Skeletal Muscle Same day prior to sampling. Antibody in Dilutions

PAGE 311 OF 399 This test Submit first morning Sputum on 3 consecutive days includes 4 Drug OR 5–10 mL Body fluids / Aspirates OR 2 mL CSF, Sensitivity for OR 100 ml (50 ml min) first morning Urine collected MYCOBACTERIU MTB Complex if on 3 consecutive days OR Fresh tissue biopsies Antitubercular Drug Sample Daily by M positive. including endometrial aspirate / curettage in sterile Sensitivity includes 11 am ; Report TUBERCULOSIS, Antitubercular normal saline OR formalin fixed paraffin embedded Rifampicin, N001 Real Time PCR Next day if PCR; Drug Sensitivity Tissue block OR 3 mL (1 mL min.) Whole Isoniazid, negative & 4 MYCOSURE; TB includes blood/Bone Marrow in 1 Lavender Top (EDTA)/ Ethambutol & days if Positive PCR Rifampicin, Yellow Top (ACD) tube OR Menstrual blood in a Pyrazinamide Isoniazid, sterile screw capped container OR culture Ethambutol & isolates.All specimens to be collected aseptically Pyrazinamide and shipped refrigerated. DO NOT FREEZE.

Submit first morning Sputum (3 consecutive days MYCOBACTERIU preferred) OR 5-10 ml Body fluid/Aspirate OR 2 ml Transcription M CSF OR Fresh tissue biopsies including endometrial Mediated Sample by Mon TUBERCULOSIS, aspirate/curettage in sterile normal saline OR 10 ml Amplification (TMA) /Wed/ Frit 11 N094 rRNA (5 mL min.) Whole Blood in 3 lavender top (EDTA) and Hybridization am, Report DETECTION, tubes OR Menstrual blood in a sterile screw capped Protection Assay Tue/Thu / Sat TMA container OR culture isolates. Ship refrigerated or (HPA) frozen.

PAGE 312 OF 399 First morning Sputum on 3 consecutive days OR 5–10 mL Body fluids/ aspirate OR 2 mL CSF OR 100 Specimens to be mL first morning Urine collected on 3 consecutive collected in 2 *Mycobacterium days OR Fresh tissue biopsies including Sample Daily by separate vials for MYCOBACTERIU Tuberculosis, endometrial aspirate / curettage in sterile normal Real Time PCR, 3.30 pmReport culture & PCR. Z277 M COMBINED PCR *Culture, saline OR 1-5 mL Whole blood / Bone Marrow in 1 Automated Interim: 14 days Sputum and urine PANEL;TB. COM AFB Rapid *Ziehl Lavender Top (EDTA) / Yellow Top (ACD) tube OR 1- fluorescent, ICT & 4 weeksFinal: 6 samples should Neelson Stain 2 mL Menstrual blood in a sterile screw capped weeks ideally be tested on container AND 3 mL (1 mL min.) blood in Myco F / 3 consecutive days. Lytic bottle available from LPL.All specimens to be collected aseptically and shipped refrigerated.

MYCOPLASMA PNEUMONIAE Sample by Mon 9 2 mL (1 mL min.) serum from 1 SST.Ship refrigerated Chemiluminescent Z677 IgG & IgM, am; Report Same or frozen. Immunoassay ANTIBODIES day PANEL, SERUM

MYCOPLASMA Sample by Mon 9 2 mL (1 mL min.) serum from 1 SST.Ship refrigerated Chemiluminescent S223 PNEUMONIAE am; Report Same or frozen. Immunoassay IgG, SERUM day

MYCOPLASMA Sample by Mon 9 2 mL (1 mL min.) serum from 1 SST.Ship refrigerated Chemiluminescent S224 PNEUMONIAE am; Report Same or frozen. Immunoassay IgM, SERUM day

3 mL ( 2 mL min.) Whole blood in 1 Lavender Top (EDTA) tube. Separate plasma within 2 hours. Ship Sample Daily by 4 MYCOPHENOLIC G187 refrigerated. Ideal sampling time 1 hour before next LC-MS/MS pm; Report Same ACID dose (Trough level) at steady state. Provide date day and time of sampling, amount and time of dose.

PAGE 313 OF 399 Also see MULTIPLE 2 mL (1 mL min.) Cerebrospinal fluid in a sterile SCLEROSIS PANELs; Sample by 7th of screw capped container. Ship refrigerated or frozen. MYELIN BASIC MYELIN ASSOCIATED the month; RW135 Blood contamination and hemolysis may interfere Immunoassay PROTEIN; MBP GLYCOPROTEIN and Report after 2–3 with the results. Ideally submit 4th lumbar puncture MAG-SGPG weeks collection tube to minimize contamination. AUTOANTIBODY.

MYELIN ASSOCIATED GLYCOPROTEIN- SULFATED Sample by 7th of 2 mL (1 mL min.) serum from 1 Red Top (No GLUCURONIC Immunoassay, the month; RW134 Additive) tube. Ship refrigerated or frozen. PARAGLOBOSID Endpoint Titer Report after 2–3 Overnight fasting is preferred. E weeks AUTOANTIBODY (MAG-SGPG), IgM MYELIN Sample by 7th of ASSOCIATED 2 mL (1 mL min.) serum from 1 Red Top (No the month; RW133 Immunoassay GLYCOPROTEIN Additive) tube. Ship refrigerated or frozen. Report after 2–3 (MAG), IgM weeks MYELOPEROXID 3 mL (2 mL min.) whole blood from 1 Lavender Top ASE (MPO) (EDTA) tube OR 2 mL (1 mL min.) Bone Marrow Sample Daily by 6 Cytochemical H225 STAIN, BLOOD from 1 Green Top (Sodium heparin) tube. Ship pm; Report 3 staining / BONE refrigerated. DO NOT FREEZE. Clinical history days MARROW mandatory. MYOGLOBIN, 2 mL (1 mL min.) serum from 1 SST.Ship refrigerated Electrochemilumines B102 Daily SERUM or frozen. cence

PAGE 314 OF 399 *Mi-2 *Ku* PM- SCL 100 * PM- Sample by Thu 9 MYOSITIS SCL 75* Jo-1 * 2 mL (1 mL min.) serum from 1 SST.Ship refrigerated S245 Immunoblot am; Report Same PROFILE SRP* PL-7 * PL- or frozen. day 12* EJ* OJ* Ro- 52 2 mL (1 mL min.) serum from 1 SST AND 3 mL (1mL min.) whole blood in 1 lavender top (EDTA) tube AND 2 mL (1 mL min.) plasma each for Fasting & NAFLD (NON *AST *ALT Post prandial from 1 Grey Top (Sodium Fluoride) Sample Mon ALCOHOLIC IFCC, Hexokinase, *Albumin* tube. Overnight Fasting is mandatory. Collect through Sat 9 Z836 FATTY LIVER BCG, Electrical Glucose F & PP fasting Glucose sample. Ask patient to have normal am;Report Same DISEASE) Impedence *Platelet Count to heavy meal. Note the time when patient starts day FIBROSIS SCORE meal, collect PP sample exactly after 2 hrs. Ship referigerated. DO NOT FREEZE. Patient's age, height & weight are mandatory. 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 Green Top (Sodium Heparin) tube OR 2 mL(1 mL Sample Daily by 9 NATURAL *CD3 *CD16 / Also see IMMUNE Z166 min.) heparinized Bone Marrow. Ship immediately Flow Cytometry am; Report Same KILLER CELLS CD56 DEFICIENCY PANELS at 18-22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. NEISSERIA 15 ml (5 mL min.) First morning Urine in a sterile Sample Daily by GONORRHEA, N088 screw capped container OR Vagina / Endocervical Real Time PCR 11 am; Report PCR Swab. Ship refrigerated. DO NOT FREEZE. Next day QUALITATIVE

PAGE 315 OF 399 NSE is high in NEURON platelets and RBC, 2 mL (1 mL min.) serum from 1 SST. Hemolysed Sample by Mon SPECIFIC Electrochemilumines therefore plasma F019 specimens not acceptable. Ship refrigerated or 4 pm; Report ENOLASE (NSE), cence and hemolysed frozen. Same day SERUM specimens are unacceptable.

*Adenovirus*Cyt omegalovirus*En teroviruses*Epst ein Barr NEUROVIRUSES virus*Herpes 3 ml (2 mL min.) Whole blood from 1 Lavender Top Sample by PANEL, Z851 simplex virus 1 & (EDTA) tube OR 3 ml (2 mL min.) CSF. Ship Real Time PCR Mon/Thu 11 am; QUALITATIVE 2*Varicella refrigerated. DO NOT FREEZE. Report Wed/Sat PCR Zoster virus*Parechovir uses*HHV6*HHV 7*Parvovirus B19

50 mL (10 mL min.) aliquot of 24-hour urine. Collect urine with appropriate volume of 50% HCl to maintain pH between 1–2. Do not use *Vanil Mandelic concentrated HCl. Record 24 hour volume on test NEUROBLASTO High Performance Sample by Wed Acid (VMA) request form and urine container. Ship Also see NEURON Z184 MA PROFILE, Liquid / Sat 9 am; *Homovanilic refrigerated or frozen. Patient should strictly avoid SPECIFIC ENOLASE. 24-HOUR URINE Chromatography Report Fri / Tue Acid (HVA) L-Dopa, Theophylline, chocolate, vanilla, banana, alcoholic beverages, tea/coffee, tobacco and strenuous exercise at least 72 hrs before and during specimen collection.

PAGE 316 OF 399 *Amphiphysin NEURONAL *CV2 *PNMA2 Sample by Mon (PARANEOPLAS (Ma2 / Ta) 2 mL (1 mL min.) serum from 1 SST. Ship through Sat 9 S232 TIC) Immunoblot *ANNA-1 / Hu refrigerated or frozen. am; Report AUTOANTIBODI *ANNA-2 / Ri Same day ES PROFILE *PCA-1 / Yo *G6PD *TSH *Phenylalanine 1 drop of heel prick blood each on 3 spots of Sample by Tue / NEWBORN *Cystic Fibrosis filter paper available from LPL. Clinical details and Z335 Fluoroimmunoassay Thu / Sat 5 pm; SCREENING *17- OHP drug history must accompany sample. Ship Report 2 days *Galactosemia refrigerated or frozen. *Biotinidase *Biotinidase *Cystic Fibrosis *G6PD *Galactosemia NEWBORN 1 drop of heel prick blood each on 3 spots of filter Sample by Tue / *17-Hydroxy TMS, G146 SCREENING, paper available from LPL.Clinical details and drug Thu / Sat 5 pm; Progesterone Fluoroimmunoassay EXTENDED history must accompany sample. Ship refrigerated. Report 2 days *TSH *Tandem Mass Spectrometry - 30 Disorders NGAL (NEUTROPHIL Sample by Mon / GELATINASE - 10 mL(5 mL min) aliquot of random urine in a sterile R167 CMIA Wed / Fri 4 pm; ASSOCIATED screw capped container. Ship refrigerated or frozen. Report Same Day LIPOCALIN), URINE

PAGE 317 OF 399 Use only specified 10 mL (5 mL min.) aliquot of 24-hour urine containers available collected in an acid washed (metal free) from LPL. If container kit available from LPL. Mix thoroughly. gadolinium or iodine Transfer 10 mL aliquot to vial provided in the Sample Daily by NICKEL, 24- containing contrast C051 kit. Measure 24 hour total volume and record ICPMS 6 pm; Report HOUR URINE media has been on vial and test request form. Ship refrigerated Next day administered, the or frozen. Patients should refrain from mineral specimen should supplements 3 days prior to specimen collection. not be collected for Use powderless gloves during specimen collection. 96 hours.

Use only specified containers available 10 mL (5 mL min.) aliquot of random urine from LPL. If collected in an acid washed (metal free) gadolinium or iodine NICKEL, Sample Daily by container available from LPL. Ship refrigerated or containing contrast C056 RANDOM ICPMS 6 pm; Report frozen. Patients should refrain from mineral media has been URINE next day supplements 3 days prior to specimen collection. administered, the Use powderless gloves during specimen collection. specimen should not be collected for 96 hours.

Sample by 7th of NICOTINE AND 10 mL (5 mL min.) random urine in a sterile screw the month; RW138 COTININE, LC-MS/MS capped container. Ship refrigerated or frozen. Report after 2–3 URINE weeks

PAGE 318 OF 399 NIEMANN PICK 10 mL (7.5 mL min.) whole blood from 3 Lavender Sample Daily by DISEASE, Top (EDTA) / Green Top (Sodium Heparin) tubes. G126 Enzyme assay 4 pm; Report 4 QUANTITATIVE, Ship refrigerated. DO NOT FREEZE. Provide brief days BLOOD clinical history Also see AMINO Sample Daily by 4 NITROPRUSSIDE 10 mL (5 mL min.) aliquot of random urine. Ship ACID PANELS, G143 Chemical pm; Report Same TEST, URINE refrigerated or frozen. QUALITATIVE AND day QUANTITATIVE. Also see AMINO Sample Daily by 4 NITROSONAPHT 10 mL (5 mL min.) aliquot of random urine. Ship ACID PANELS, G142 Chemical pm; Report Same HOL, URINE refrigerated or frozen. QUALITATIVE AND day QUANTITATIVE. *KOH Calcifluor Submit Sputum/ BAL /PUS/ Swab /Endotracheal Sample Daily by 9 NOCARDIA, * Gram stain M185 tube secretions / Swabs / Culture Growth of Light microscopy am; Report STAIN *Modified ZN Nocardia. Ship at 18-22°C Same day stain

3 mL (1.5 mL min.) serum from 1 Red Top (No Sample by 7th of Additive) tube. Do not use SST gel barrier tubes. the month; RW140 NORTRIPTYLINE LC-MS/MS Ship refrigerated or frozen. Recommended Report after 2–3 Specimen: >12 hours post oral dose. weeks

5 mL (3 mL min.) Whole blood / Bone marrow in 1 Sample by Mon / NPM1 GENE N051 Lavender Top (EDTA) tube. Ship refrigearted. DO Real Time PCR Thu 11 am; MUTATION NOT FREEZE. ReportWed / Sat NRAS Submit formalin fixed paraffin embedded tissue MUTATION Sample by Tue 11 N108 block. Ship at room temperature. Block should have PCR, Sequencing CODON 12, 13 am; Report Mon at least 10% tumor tissue. & 61

PAGE 319 OF 399 NT-PRO BNP (N- TERMINAL PRO 2 mL (1 mL min.) serum from 1 SST. Ship B185 B TYPE ECLIA Daily refrigerated or frozen. NATRIURETIC PEPTIDE)

Also see ALKALINE NTx 10 mL (5 mL min.) aliquot of 24-hour / Second void PHOSPHATASE, (COLLAGEN urine in a sterile screw capped container. Do not BONE SPECIFIC; Sample by Sat 9 CROSS-LINKED Enzyme B161 use preservatives. Ship refrigerated or frozen. OSTEOPOROSIS am; Report Same N- Immunoassay Record24 hour volume on vial and Test Request PANEL; PYRIDINIUM day TELOPEPTIDE), Form. COLLAGEN CROSS URINE LINKS.

Test should not be performed on Submit 10 mL random urine collected between persons with Total midnight and noon as a single void. Stabilize NUCLEAR cystectomy. Urine Sample by 7th immediately after collection using the special NMP MATRIX samples should be of the month; RW141 urine collection kit available from LPL. Follow Immunoassay PROTEINS; collected at least 5 Report instructions provided with the kit carefully. NMP days after invasive after 2–3 weeks Stabilized urine will turn blue / green in color. Keep procedures like away from sunlight. Ship refrigerated or frozen. Cystoscopy or Catheterization.

3 mL (1.5 mL min.) serum from 1 SST AND 2 mL (1 * Sugar (F) *TSH mL min.) Plasma from 1 Grey Top (Sodium Fluoride) Spectrophotometry, Sample by Sat 9 Z321 OBESITY PANEL *Lipid profile tube. Hemolysed specimens not acceptable. Ship ChemiluminescentIm am; Report Same Basic *Leptin refrigerated or frozen. Minimum 12 hours fasting is munoassay, EIA day mandatory

PAGE 320 OF 399 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 Sample Daily by 9 OBSTETRIC *Natural Killer Green Top (Sodium Heparin) tube. Ship immediately Z292 Flow Cytometry am; Report Same FLOW PANEL (NK) cells *CD 19 at 18–22°C. DO NOT REFRIGERATE OR FREEZE. day Specify time, date and clinical details on test request form. Cervical swab / cells in special container with medium available from LPL. Ship refrigerated. DO Sample by Tue ONCOGENIC NOT FREEZE. Store specimen at 2-8 degrees celsius /Thu / Sat 9 am; Z818 Flow Cytometry E6/E7 mRNA immediately after collection. Exposure of the Report Wed / Fri specimen to high temperatures has adverse effects / Mon on sample quality. OPIATES SCREEN, 15 mL (10 mL min.) aliquot of random urine. Ship Lateral Flow K001 Daily RANDOM refrigerated or frozen. Immunoassay URINE ORGANIC 15 mL (10 mL min.) aliquot of random urine in a ACIDS, sterile screw capped container without Gas Sample Daily by 5 QUANTITATIVE, G164 preservative. Ship refrigerated or frozen. Clinical Chromatography / pm: Report 5 RANDOM details and drug history must accompany the Mass Spectrometry days URINE, FULL sample. PANEL 15 mL (10 mL min.) aliquot of random urine. Ship Sample Daily by 4 G150 OROTIC ACID refrigerated of frozen.Collect specimen fasting or 2- GC-MS pm; Report 2 3 hours post feed days 3 mL (1.5 mL min.) serum from 1 SST AND 2 mL (1 Freezing Point *Sodium *BUN mL min.) plasma from 1 Grey Top (Sodium Sample Daily by OSMOLAL GAP, Depression, B184 *Glucose R Fluoride) tube. Separate serum / plasma within 1 4 pm; Report SERUM Spectrophotometry, *Osmolality hour of collection. Ship refrigerated or frozen. Same day Calculated Grossly hemolysed samples not accepted.

PAGE 321 OF 399 2 mL (1 mL min.) serum from 1 SST. Separate serum Sample Daily by OSMOLALITY, Freezing Point Also see ARGININE P064 within 1 hour of collection. Ship refrigerated or 4 pm; Report SERUM Depression VASOPRESSIN. frozen. Grossly hemolysed samples not accepted. Same day

5 mL (3 mL min.) aliquot of random urine. Do not Sample Daily by OSMOLALITY, Freezing point Also see ARGININE U040 use preservative. Ship refrigerated. DO NOT 4 pm; Report URINE depression VASOPRESSIN. FREEZE. Same day

2 mL (1.5 mL min.) serum from 1 SST AND 5 mL (3 OSMOLALITY, Sample Daily by mL min.) aliquot of random urine. Do not use Freezing point U100 URINE : SERUM 4 pm; Report preservative. Separate serum within 1 hour of depression RATIO Same day collection. Ship refrigerated. DO NOT FREEZE.

OSMOTIC 3 mL (2 mL min.) whole blood from 1 Green Top Oxalate and citrate Sample Daily by FRAGILITY H012 (Sodium Heparin) tube.Ship refrigerated. DO NOT Parparts samples not 12 noon; Report TEST; RBC FREEZE. accepted. Next day FRAGILITY TEST

*Calcium *NTX 5 mL (3 mL min.) serum from 2 SST’s. Collect sample *Estradiol between 8-10 a.m, separate serum and freeze *Osteocalcin within 30 minutes AND 20 mL (10 mL min) aliquot Arsenazo III, CLIA, Sample by Sat 9 OSTEOPOROSIS Z159 *PTH-Intact of first morning urine without preservatives. Avoid ECLIA, Molybdate am; Report in PANEL *Vitamin D, 25- use of tourniquet and fist clenching during sampling. UV, EIA two days Hydroxy Overnight fasting is preferred. Ship frozen. DO NOT *Phosphorus THAW. Hemolysed samples are not acceptable.

*Calcium 4 mL (2 mL min.) serum from 1 SST. Collect sample *Phosphorus between 8-10 a.m, in a fasting condition, separate OSTEOSCREEN CLIA, Z612 *PTH-Intact serum and freeze within 30 minutes. Avoid use of Daily PANEL Spectrophotometry *Vitamin D, 25- tourniquet and fist clenching during sampling. Ship Hydroxy frozen. DO NOT THAW.

PAGE 322 OF 399 OSTEOCALCIN; 2 mL (1 mL min.) serum from 1 SST. Separate serum Sample by Mon 4 Electrochemilumines R108 BONE Gla within 1 hour and freeze. Ship frozen. DO NOT pm; Report Same cent Immunoassay PROTEIN (BGP) THAW. Overnight fasting is preferred. day

*FSH *Estradiol 3 mL (2 mL min.) serum from 1 SST. Ship Sample Daily by 9 OVARIAN Z606 *Anti Mullerian refrigerated or frozen. Specify age, sex and day of EIA, CLIA am; Report Same RESERVE PANEL Hormone menstrual cycle on test request form. day

Sample by Mon 9 OVARIAN *CA 125 *Inhibin 4 mL ( 2 mL min.) serum from 1 SST. Ship Z803 CMIA, EIA am; Report Same CANCER PANEL B refrigerated or frozen. day

10 mL (5 mL min.) aliquot of 24 hour urine. Collect urine with 25 mL 50% concentrated HCl to maintain pH below 3. For children adjust the volume of acid according to the expected urinary output. Keep Sample by Wed / OXALATE, 24 U055 urine refrigerated during collection. Mix well before Spectrophotometry Sat 9 am; HOUR URINE aliquoting. Record body weight & 24 hour volume ReportSame day on container and test request form. Ship refrigerated or frozen. Avoid vitamin C and citrus fruits 48 hours prior to specimen collection.

10 mL (5 mL min.) aliquot of random urine in 2 mL OXALATE, of 6N HCl to maintain pH below 3. Mix well Sample by Wed U101 RANDOM before aliquoting. Ship refrigerated or frozen. Avoid Spectrophotometry / Sat 9 am; URINE vitamin C and citrus fruits 48 hrs prior to Report Same day specimen collection.

PACKED CELL Submit 3 mL (1.5 mL min.) body fluids in a sterile P093 VOLUME (PCV), screw capped container. Ship refrigerated. DO NOT Calculated Daily BODY FLUIDS FREEZE

PAGE 323 OF 399 PACKED CELL 3 mL (1 mL min.) whole blood in 1 Lavender H002 VOLUME (PCV); Top (EDTA) tube. Ship refrigerated. DO NOT Calculated Daily HEMATOCRIT FREEZE Sample by 7th 2 mL (1 mL min.) plasma from 1 Lavender Top PANCREATIC Extraction, of the month; RW144 (EDTA) tube. Ship refrigerated or frozen. POLYPEPTIDE Radioimmunoassay Report after 2–3 Overnight fasting is preferred. weeks PAN FUNGAL DETECTION 4 mL (2 mL min.) Whole Blood in 1 Lavender top Sample by Thu OS175 AND (EDTA) Tube OR 2 mL CSF/Sputum/ Fluids OR Tissue PCR 11 am; Report 8 IDENTIFICTION, in Normal saline. Ship refrigerated. DO NOT FREEZE. days PCR PAN FUNGAL 4 mL (2 mL min.) Whole Blood in 1 Lavender top Sample by Thu OS174 DETECTION , (EDTA) Tube OR 2 mL CSF/Sputum/ Fluids OR Tissue PCR 11 am; Report 8 PCR in Normal saline. Ship refrigerated. DO NOT FREEZE. days PAPP-A (PREGNANCY Also see MATERNAL Sample Daily by 2 mL (1 mL min.) serum from 1 SST. Ship B152 ASSOCIATED CLIA SERUM SCREEN 2; 9 am; Report refrigerated or frozen. PLASMA DUAL TEST Same day PROTEIN-A) 2 mL (1 mL min.) serum from 1 SST. Ship For SERIAL Sample Daily by PARIETAL CELL Immunofluorescence S086 refrigerated or frozen. Overnight fasting is DILUTIONS, use 11 am; Report ANTIBODY, IFA Assay preferred. test code S087. Same day

PARIETAL CELL 2 mL (1 mL min.) serum from 1 SST. Ship Sample Daily by Immunofluorescence S087 ANTIBODY, IFA refrigerated or frozen. Overnight fasting is 11 am; Report Assay IN DILUTIONS preferred. Next day

PAGE 324 OF 399 PAROXYSMAL 3 mL (2 mL min.) whole blood in 1 Lavender *CD55 * FLAER NOCTURNAL Top (EDTA) tube AND 3 mL (2 mL min.) whole on Monocytes Sample Daily by HEMOGLOBINU blood in 1 Green Top (Sodium Heparin) tube. Z496 and Flow Cytometry 9 am; Report RIA (PNH), Ship immediately at 18–22°C. DO NOT REFRIGERATE Granulocytes Same day CONFIRMATOR OR FREEZE. Specify time, date and clinical details *CD59 on RBC Y TEST on test request form. Sample by 7th 2 mL (1 mL min.) serum from 1 Red Top (No of the month; RW145 PAROXETINE Additive) tube. Separate serum within 2 hours of LC-MS/MS Report after 2–3 collection. Ship refrigerated or frozen. weeks

3 mL Whole blood in 1 Blue Top (Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hrs. If this is not possible, make PPP within 1 hour of collection PARTIAL as follows: Centrifuge sample at 3600 rpm for Also see LUPUS THROMBOPLAS 15 min & transfer supernatent to a clean plastic Photo-optical Clot ANTICOAGULANT BY H014 TIN TIME, Daily tube. Centrifuge this supernatant again at 3600 Detection APTT and MIXING ACTIVATED; rpm for 15 mins & finally transfer the supernatant STUDIES. PTT; APTT (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen.DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form is mandatory.

PARVOVIRUS B19 Sample by Fri 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z185 ANTIBODIES am; Report refrigerated or frozen. Immunoassay PANEL, IgG & Same day IgM PARVOVIRUS Sample by Fri 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S142 B19 ANTIBODY, am; Report refrigerated or frozen. Immunoassay IgG Same day

PAGE 325 OF 399 PARVOVIRUS 3 ml (2 mL min.) whole blood from 1 Lavender Top Sample by B19, N124 (EDTA) tube OR 3 ml (2 mL min.) CSF. Ship Real Time PCR Mon/Thu 11 am; QUALITATIVE refrigerated. DO NOT FREEZE. Report Wed/Sat PCR PARVOVIRUS Sample by Fri 9 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S143 B19 am; Report refrigerated or frozen. Immunoassay ANTIBODY, IgM Same day

3 mL (2 mL min.) whole blood from 1 Lavender Top PAS (PERIODIC (EDTA) tube OR 2 mL (1 mL min.) Bone Marrow Sample Daily by ACID SCHIFF) Cytochemical H224 from 1 Green Top (Sodium heparin) tube. Ship 6 pm; Report 3 STAIN, BLOOD / staining refrigerated. DO NOT FREEZE. Clinical history is days BONE MARROW mandatory.

PCNA (PROLIFERATIN Sample by 7th G CELL 2 mL (1 mL min.) serum from 1 Red Top (No Immunofluorescence of the month; RW146 NUCLEAR Additive) tube. Ship refrigerated or frozen. Assay Report after 2–3 ANTIGEN), weeks ANTIBODY

*FSH *LH *Testosterone, 4 mL (2 mL min.) serum from 1 SST AND 2 mL (1 mL PCOD Total *Prolactin min.) plasma from 1 Grey Top (Sodium Fluoride) (POLYCYSTIC Hexokinase, CLIA, B170 *Insulin, fasting tube. Collect specimen 4 hrs after the patient Daily OVARIAN CMIA *Glucose, has awakened. Ship refrigerated or frozen. DISEASE) PANEL fasting * TSH Overnight fasting is mandatory. Ultrasensitive

PAGE 326 OF 399 *FSH *LH *Testosterone, Total *Prolactin PCOD *Insulin, fasting 2 mL (1 mL min.) plasma from Gray Top (Sodium (POLYCYSTIC *Glucose, Fluoride) tubes for Glucose AND 4 mL (2 mL min.) Hexokinase, CMIA, Sample Daily by 4 OVARIAN Z838 fasting * TSH serum from 1 SST; .Ship refrigerated. Overnight CLIA,Spectrophotom pm; Report Same DISEASE) Ultrasensitive fasting is mandatory. Collect specimen 4 hrs after etry day COMPREHENSIV *DHEAS *AMH the patient has awakened E PANEL *Lipid Profile, Screen *HOMA- IR

Submit 10 mL (5 mL min.) aliquot of 24 hour Peritoneal dialysate fluid in a sterile screw capped PERITONEAL container at 0 hour, 2 hour, 4 hour, overnight & EQUILIBRIUM 24 hour intervals AND 10 mL (5 mL min.) aliquot of Z517 Spectrophotometry Daily & ADEQUACY 24 hour Urine in a sterile screw capped TEST container AND 2 mL (1 mL min.) serum from 1 SST & 2 mL (1 mL min.) plasma from 1 Grey Top (Sodium flouride) tube

Submit 10 mL (5 mL min.) aliquot of Peritoneal dialysate fluid in a sterile screw capped container PERITONEAL at 0 hour, 2 hour, 4 hour & overnight intervals Z515 EQUILIBRIUM Spectrophotometry Daily AND 2 mL (1 mL min.) serum from 1 SST & 2 mL (1 TEST (PET) mL min.) plasma from 1 Grey Top (Sodium flouride) tube both along with 2 hour samples only.

PAGE 327 OF 399 *Vitamin B12 *Gastrin 5 mL (3 mL min.) serum from 1 SST. Wrap sample in Sample by Mon 9 PERNICIOUS *Intrinsic Factor aluminium foil to protect from light.Ship Z826 EIA, CLIA, IFA am; Report Same ANEMIA PANEL Antibody refrigerated or frozen. Overnight fasting is Day *Parietal Cell mandatory. Antibody

PHENCYCLIDINE 15 mL (10 mL min.) aliquot of random urine. Ship Lateral Flow K016 (PCP), Daily refrigerated or frozen. Immunoassay SCREEN, URINE 2 mL (1 mL min.) serum from 1 RedTop (No Additive) tube. Do not use SST gel barrier tubes. PHENOBARBITO Chemiluminescent D050 Ship refrigerated or frozen. Recommended Daily NE Immunoassay specimen: just before the next dose is due at steady state. 10 ml (5 ml min.) urine sample collected at the end Sample by Tue 7 V603 PHENOL of the shift on the last working day of the week. HPLC am; Report 2 Ship refrigerated or frozen. weeks 1 drop of heel prick blood each on 3 spots of Sample by Tue PHENYLALANIN filter paper available from LPL. Clinical details and / Thu / Sat 5 R160 E, NEWBORN Fluoroimmunoassay drug history must accompany sample. Ship pm; Report 2 SCREEN refrigerated or frozen. days 2 mL (1 mL min.) serum from 1 Red Top (No Additive) tube. Do not use SST gel barrier tubes. Chemiluminescent D049 PHENYTOIN Ship refrigerated or frozen. Recommended Daily Immunoassay specimen: just before the next dose is due at steady state.

PAGE 328 OF 399 2 mL ( 1 mL min.) plasma from 1 Lavender Top EDTA tube AND 50 mL (10 mL min.) aliquot of 24- hour urine. Collect urine with appropriate volume of 50% HCl to maintain pH between 1–2. Do not use concentrated HCl. Record 24 hour volume on test request form and urine container. Ship frozen. DO NOT THAW. Patient should strictly avoid Alpha-one blockers, Aminophylline, Amphetamines, *VMA Ampicillin, Beta-blockers, Ephedrine, Imipramine, High Performance PHEOCHROMO *Metanephrines Methyldopa, Buspiron, Codeine, L-Dopa, MAO Liquid Also see Sample by Fri 9 Z144 CYTOMA *Catecholamines inhibitors, Nicotine, Phenacetin, Phenothiazine, Chromatography, CHROMOGRANIN A. am; Report Tue PROFILE * Chromogranin Theophylline, Vasodilators, Aspirin,PAS, Alpha-two EIA A agonists, Bromocriptine, Calcium channel blockers (long term use), Clofibrate, Propranolol, Penicillin, Reserpine, Thyroxine, Alpha-methyldopa, Isoproterenol, Labetalol, Mandelamine, Paracetamol, Cimetidine, Metoclopramide, vigorous exercise, alcoholic beverages, tea/coffee, tobacco, chocolate, pepper, vanilla & banana at least 72 hours before and during specimen collection.

PHOSPHATIDYL SERINE 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Sample by Tue 11 Z167 ANTIBODIES refrigerated or frozen. Immunoassay am; Report Fri PANEL, IgG & IgM PHOSPHOLIPID Sample by Mon ANTIBODIES 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme through Sat 9 Z287 PANEL, IgG & refrigerated or frozen. Immunoassay am; Report Same IgM day

PAGE 329 OF 399 It is recommended 4 mL (2.5 mL min) serum from 1 SST AND 3 mL that patient Whole blood in 1 Blue Top (Sodium Citrate) discontinues tube. Mix thoroughly by inversion. Transport to Heparin for 1 day *Cardiolipin Lab within 4 hrs. If this is not possible, make and Oral Antibodies IgG PPP within 1 hour of collection as follows: Anticoagulants for 7 & IgM PHOSPHOLIPID Centrifuge sample at 3600 rpm for 15 min & EIA, days prior to Sample Daily by *Phospholipid Z377 SYNDROME transfer supernatent to a clean plastic tube. Electromechanical sampling as these 3 pm; Report Antibodies PANEL Centrifuge this supernatant again at 3600 rpm for Clot Detection drugs may affect Next day Panel *Lupus 15 mins & finally transfer the supernatant (PPP) test results. Anticoagulant to 1 labelled, clean plastic screw capped vial. Discontinuation by dRVVT FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. should be with prior Overnight fasting is preferred. Duly filled consent from the Coagulation Requisition Form is mandatory treating Physician. Also see Ç

PHOSPHATIDYL 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Sample by Tue 11 S120 SERINE refrigerated or frozen. Immunoassay am; Report Fri ANTIBODY, IgG PHOSPHOLIPAS E A2 2 mL (1 mL min.) serum from 1 SST. Ship Sample by Mon 9 S240 RECEPTOR IFA refrigerated or frozen. Overnight fasting is preferred. am; Report Wed ANTIBODY (PLA2R) Sample by Mon PHOSPHOLIPID 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme through Sat 9 S191 ANTIBODY, IgG refrigerated or frozen. Immunoassay am; Report Same day

PAGE 330 OF 399 PHOSPHORUS, 10 mL (5 mL min.) aliquot of random urine. Do not U074 RANDOM Molybdate UV Daily use preservatives. Ship refrigerated or frozen. URINE 2 mL (1 mL min.) serum from 1 SST. Ship PHOSPHORUS, B043 refrigerated or frozen. Overnight fasting is Molybdate UV Daily SERUM preferred. PHOSPHATIDYL 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Sample by Tue 11 S121 SERINE refrigerated or frozen. Immunoassay am; Report Fri ANTIBODY, IgM 10 mL (5 mL min.) aliquot of 24-hour urine. Do not PHOSPHORUS, use preservatives. Record 24 hour volume on U012 24-HOUR Molybdate UV Daily test request form and urine container. Ship URINE refrigerated or frozen. Sample by Mon PHOSPHOLIPID 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme through Sat 9 S192 ANTIBODY, IgM refrigerated or frozen. Immunoassay am; Report Same day Sample by 7th 3 mL (1.5 mL) serum from 1 Red Top (No Additive) PIGEON of the month; RW262 Tube.Do not use SST gel barrier tubes. Ship Immunoassay FEATHERS IgE Report after 2–3 refrigerated or frozen. weeks Sample by 7th 1 mL (0.5 mL) serum from 1 Red Top (No Additive) PIGEON of the month; RW261 Tube. Do not use SST gel barrier tubes. Ship Immunoassay DROPPINGS, IgE Report after 2–3 refrigerated or frozen. weeks PIK3CA Submit formalin fixed paraffin embedded tissue Sample by Tuey N114 MUTATION block. Ship at room temperature. Block should have PCR, Sequencing 11 am; Report ANALYSIS at least 10% tumor tissue. Mon

PAGE 331 OF 399 PLASMINOGEN ACTIVATOR Sample by Wed INHIBITOR- 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS178 PCR 11 am; Report 8 1/SERPINE-1, (EDTA) tube. Ship refrigerated. DO NOT FREEZE. days 4G/5G GENOTYPING 3 mL (2.5 mL min.) plasma from 1 Lavender Top (EDTA) tube. Collect blood between 8 am to 10 am. Patient should be ambulatory / upright 2 hours prior to the test. Separate plasma and FREEZE. Ship Sample by Fri 9 PLASMA RENIN, frozen. DO NOT THAW. Overnight fasting is Also see R072 CLIA am; Report DIRECT preferred. When submitting catheterization ANGIOTENSIN II. Same day studies, it is recommended that the referring laboratory retain a portion of each sample. No medication 3 weeks prior to draw is recommended.

PLASMINOGEN 2 mL (1 mL min.) plasma from 1 Blue Top (Sodium Sample by 7th ACTIVATOR Citrate) tube. Separate plasma & freeze of the month; RW147 Immunoassay INHIBITOR-1 immediately. Ship frozen. DO NOT THAW. Report after 2–3 (PAI-1) ACTIVITY Overnight fasting is mandatory. weeks PLATELET 3 mL (1 mL min.) whole blood in 1 Lavender COUNT, Fluorescent Flow H251 Top (EDTA) tube. Ship refrigerated. DO NOT Daily FLUORESCENT Cytometry FREEZE. (PLT-F) 3 mL (1 mL min.) whole blood in 1 Lavender PLATELET H010 Top (EDTA) tube. Ship refrigerated. DO NOT Electrical Impedence Daily COUNT FREEZE. PLATELET 3 mL (1 mL min.) whole blood in 1 Lavender H210 DISTRIBUTION Top (EDTA) tube. Ship refrigerated. DO NOT Electrical Impedence Daily WIDTH; PDW FREEZE.

PAGE 332 OF 399 PML RARA t(15;17)(q22;q1 Sample by Mon 2) GENE 5 mL (3 mL min.) whole blood from 2 Lavender / Wed / Fri 9 N095 REARRANGEME Top (EDTA) tubes. Ship refrigerated. DO NOT Real Time PCR am; Report Tue / NT FREEZE. Thu / Sat QUANTITATIVE, PCR PML RARA t(15;17)(q22;q1 Sample by Mon 2) GENE 5 mL (3 mL min.) whole blood from 2 Lavender / Wed / Fri 9 N038 REARRANGEME Top (EDTA) tubes. Ship refrigerated. DO NOT Real Time PCR am; Report Tue / NT FREEZE. Thu / Sat QUALITATIVE, PCR Submit Sputum / Induced Sputum / BAL FLUID in a Sample Daily by 9 PNEUMOCYSTIS M221 sterile screw capped container. Ship refrigerated. Direct IFA am;Report Same , IFA DO NOT FREEZE. day P1NP (PROCOLLAGEN Sample by Mon TYPE 1 AMINO 2 mL (1 mL min.) serum from 1 SST. Ship Electrochemilumines B165A / Thu 4 pm; TERMINAL refrigerated or frozen. cence Report same day PROPEPTIDE), TOTAL

POLARIZING Sample Daily by 5 mL (2 mL min.) body fluids in a sterile screw Polarizing P097 MICROSCOPY, 2 pm; Report capped container. Ship refrigerated. microscopy BODY FLUIDS Same day POMPE 10 mL (7.5 mL min.) whole blood from 3 Lavender This tests deficiency Sample Daily by DISEASE, Top (EDTA) / Green Top (Sodium Heparin) tubes. G128 Enzyme assay of enzyme Alpha 4 pm; Report 4 QUANTITATIVE, Ship refrigerated within 48 hrs. Clinical history Glucosidase days BLOOD must accompany sample.

PAGE 333 OF 399 The recommended 10 mL (5 mL min.) aliquot of random urine screening test is PORPHYRINS preferably DURING ACUTE CRISIS in a dark PORPHYRINS, TOTAL, Sample Daily by coloured vial / container wrapped completely with Column TOTAL, 24 HOURS U053 QUALITATIVE, 1 pm.; Report black paper or aluminium foil. Do not use chromatography URINE. Also see 5- RANDOM Same day preservatives. Ship refrigerated or frozen. AMINOLEVULINIC URINE Uncovered vials exposed to light are unacceptable. ACID, PORPHOBILINOGEN.

10 mL (5 mL min.) aliquot of 24 hour urine Also see PORPHOBILINO collected in a dark coloured container / wrapped PORPHYRINS, Sample by Tue GEN (PBG), completely with black paper or aluminium foil. Ship Column U098 TOTAL; 5- / Fri 9 am; QUANTITATIVE, refrigerated or frozen. Record 24 hour volume Chromatography AMINOLEVULINIC Report Same day 24 HOUR URINE on test request form and urine container. ACID. Uncovered vials exposed to light are unacceptable.

10 mL (5 mL min.) aliquot of random urine PORPHOBILINO Also see collected in a dark coloured vial / container Sample by Tue GEN (PBG), Column PORPHYRINS, U029 wrapped completely with black paper or aluminium / Fri 9 am; QUALITATIVE, Chromatography TOTAL; 5-AMINO- foil. Ship refrigerated or frozen. Uncovered vials Report Same day URINE LEVULINIC ACID. exposed to light are unacceptable

10 mL (5 mL min.) aliquot of 24 hour urine in PORPHYRINS a dark coloured vial / container wrapped Also see 5- TOTAL, completely with black paper or aluminium foil. Do Sample Daily by Column AMINOLEVULINIC U003 QUANTITATIVE, not use preservatives. Ship refrigerated or frozen. 1 pm.; Report chromatography ACID, 24- HOUR Record 24 hour volume on test request form Same day PORPHOBILINOGEN. URINE and urine container. Uncovered vials exposed to light are unacceptable.

PAGE 334 OF 399 10 mL (5 mL min.) aliquot of random urine. Do POTASSIUM, not use preservative. Blood contaminated and Indirect Ion U014 RANDOM Daily highly turbid samples not acceptable. Ship Selective Electrode URINE refrigerated or frozen. 10 mL (5 mL min.) aliquot of 24-hour urine. Do not POTASSIUM, use preservative. Record 24 hour volume on Indirect Ion U058 24-HOUR container and test request form. Blood Daily Selective Electrode URINE contaminated and highly turbid samples not acceptable. Ship refrigerated or frozen 2 mL (1 mL min.) serum from 1 SST. Separate serum POTASSIUM, from cells within 1 hour of collection. Indirect Ion B045 Daily SERUM Hemolysed samples not acceptable. Ship Selective Electrode refrigerated or frozen. 2 mL (1 mL min.) fluid in a sterile screw capped POTASSIUM, container. Ship refrigerated. DO NOT FREEZE. Blood Indirect Ion P098 Daily FLUID contaminated and highly turbid samples not Selective Electrode acceptable. PRADER-WILLI SYNDROME Sample by Sat 11 MUTATION 4 mL (2 mL min.) Whole Blood in 1 Lavender top Methylation specific OS179 am; Report 11 DETECTION, (EDTA) tube. Ship refrigerated. DO NOT FREEZE. PCR days METHYLATION SPECIFIC PCR Sample by 7th 2 mL (1 mL min.) serum from 1 Red Top (No of the month; RW151 PREALBUMIN Nephelometry Additive) tube. Ship refrigerated or frozen. Report after 2–3 weeks

PAGE 335 OF 399 NICE (2016) recommends usage of sFlt-1/PlGF ratio, with standard clinical assessment PRE- *PLGF * sFLT & subsequent Sample by Tue / ECLAMPSIA 2 mL (1 mL min.) serum from 1 SST. Ship Z841 *sFLT-1 : PLGF ECLIA clinical follow-up, to Thu / Sat 9 am; PREDICTOR refrigerated or frozen. RATIO help rule-out pre- Report Same Day PANEL eclampsia in suspected women between 20 - 34 weeks+ 6 days of gestation. Sample by 7th 2 mL (1 mL min.) serum from 1 Red Top (No PREGNENOLON of the month; RW152 Additive) tube. Do not use SST gel barrier tubes. LC-MS/MS E Report after 2–3 Ship refrigerated or frozen. weeks PREGNANCY 10 mL (5 mL min.) aliquot of first void specimen of Immunochromatogr S006 Daily TEST, URINE urine. Ship refrigerated. aphy

PAGE 336 OF 399 This Panel tests the following diseases: *Metachromatic Leucodystrophy Submit 20-30 mg (15 mg min.) Chorionic villus (Code Y556) biopsy. Wash in minimal Essential media and put *GM 1 Kindly specify the PRENATAL in Culture medium. Dissect immediately to Gangliosidosis disease to be tested. DIAGNOSIS remove decidua; Wash twice in cold 0.9% Sample Daily by (Code Y557)*GM SAMPLE TO BE G133 PANEL 1, normal saline and add 0.2 mL of cold distilled Enzyme Assay 4 pm; Report 5 2 Gangliosidosis DISPATCHED WITH CHORIONIC water. Ship frozen within 24 hours. Sample days (Code PRIOR VILLUS BIOPSY should be taken at 10-13 weeks gestation. It is Y558)*Gaucher APPOINTMENT. mandatory to send a Signed Prenatal Consent Form Disease (Code with the specimen. Y559) *Niemann Pick Disease (Code Y560 )*Fabry Disease (Code Y561)*PoÇ

PRENATAL Submit 10 mL (7 mL min.) Amniotic fluid in a sterile SAMPLE TO BE DIAGNOSIS screw capped container. Ship at 18–22°C within Sample Daily by *Mucopolysacch Two Dimensional DISPATCHED WITH G134 PANEL 2, 24hrs. DO NOT FREEZE. Sample should be taken 4 pm; Report aridoses (MPS) electrophoresis PRIOR AMNIOTIC after 16 weeks gestation. It is mandatory to send 10 days APPOINTMENT. FLUID a Signed Prenatal Consent Form with the specimen.

PAGE 337 OF 399 3 mL (2 mL min.) serum from 1 SST AND 2 mL ( 1 m L min.) plasma from 1 Grey Top (Sodium Fluoride) *Blood Group tube AND 3 ml (2 mL min.) whole blood in 1 & Rh Factor Lavender Top (EDTA) tube AND 10 mL (5 mL *Urea min) aliquot of random urine in a sterile screw *Creatinine capped container Ship refrigerated wthin 48 hrs *Glucose AND 3 mL Whole blood in 1 Blue Top (Sodium Random *HIV PRE-OPERATIVE Citrate) tube. Mix thoroughly by inversion. See individual Z614 Rapid *HBsAg Daily PANEL Transport to Lab within 4 hrs. If this is not assays Rapid *HCV possible, make PPP within 1 hour of collection Rapid as follows: Centrifuge sample at 3600 rpm for *Prothrombin 15 min & transfer supernatent to a clean plastic Time tube. Centrifuge this supernatant again at 3600 *Hemoglobin rpm for 15 mins & finally transfer the supernatant *Urine R/E (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen.

2 mL (1 mL min.) serum from 1 Red Top (No Includes Sample by 7th Additive) tube. Do not use SST gel barrier tubes. metabolite N- of the month; RW153 PROCAINAMIDE Ship refrigerated or frozen. Recommended Immunoassay acetylprocainami Report after 2–3 specimen: just before the next dose is due at de weeks steady state. PROCALCITONI 2 mL (1 mL min.) serum from 1 SST. Ship Electrochemilumines B176 Daily N refrigerated or frozen cent Immunoassay 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent PROGESTERONE R011 refrigerated or frozen. Specify age, sex and Microparticle Daily , SERUM menopausal status on test request form. Immunoassay Sample by 7th 2 mL (1 mL min.) serum from 1 SST. Separate serum of the month; RW154 PROINSULIN within 1 hour of collection. Ship refrigerated or Immunoassay Report after 2–3 frozen. Overnight fasting is mandatory. weeks

PAGE 338 OF 399 Prolactin is secreted episodically. Pooling 2 mL (1 mL min.) serum from 1 SST. Collect equal volume of PROLACTIN, specimen 4 hours after the patient has awakened. Chemiluminescent R082 serum from 3 Daily SERUM Overnight fasting is preferred. Ship refrigerated or Immunoassay samples drawn at 15 frozen. minute intervals is recommended.

Sample by 7th PROPERDIN 2 mL (1 mL min.) serum from 1 Red Top (No of the month; RW155 FACTOR B; C3 Additive) tube. Separate serum within 2 hours of Nephelometry Report after 2–3 PROACTIVATOR collection. Ship refrigerated or frozen. weeks 10 mL (5 mL min.) aliquot of random urine in a PROPOXYPHENE Lateral Flow K020 sterile screw capped container. Do not use Daily SCREEN, URINE Immunoassay preservatives. Ship refrigerated or frozen.

*Protein PROTEIN & Electrophoresis, 3 mL (1.5 mL min.) serum from 1 SST. Ship Immunoturbidimetry Sample Daily by Z016 IMMUNOGLOB Serum refrigerated. or frozen. Grossly hemolysed , Capillary 9 am; Report ULIN PANEL *Immunoglobulin samples not accepted. electrophoresis Same day profile serum PROTEIN - 20 mL (10 mL min.) aliquot of first morning / U087 CREATININE random urine in a sterile screw capped Spectrophotometry Daily RATIO, URINE container. .Ship refrigerated. DO NOT FREEZE. Includes total protein, Sample Mon PROTEIN electrophoretic 2 mL (1 mL min.) serum from 1 SST. Ship Capillary through Sat by 9 E001 ELECTROPHORE fractions with refrigerated or frozen. Grossly hemolysed Electrophoresis am; Report Same SIS, SERUM densitometric samples not accepted. day tracing. Does not include IFE.

PAGE 339 OF 399 3 mL Whole blood in 1 Blue Top (Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hrs. If this is not possible, make PPP within 1 hour of collection as follows: Centrifuge sample at 3600 rpm for Sample by 7th PROTEIN S, 15 min & transfer supernatent to a clean plastic of the month; RW158 Immunoturbidimetry ANTIGEN tube. Centrifuge this supernatant again at 3600 Report after 2–3 rpm for 15 mins & finally transfer the supernatant weeks (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form is mandatory.

It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium discontinues Citrate) tube. Mix thoroughly by inversion. Heparin for 1 day Transport to Lab within 4 hrs. If this is not and Oral possible, make PPP within 1 hour of collection Anticoagulants for 7 as follows: Centrifuge sample at 3600 rpm for days prior to Sample by Thu PROTEIN C, 15 min & transfer supernatent to a clean plastic H061 Chromogenic Assay sampling as these 9 am; Report FUNCTIONAL tube. Centrifuge this supernatant again at 3600 drugs may affect Next day rpm for 15 mins & finally transfer the supernatant test results. (PPP) to 1 labelled, clean plastic screw capped Discontinuation vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT should be with prior THAW. Overnight fasting is preferred. Duly filled consent from the Coagulation Requisition Form is mandatory. treating Physician. Also see DEEÇ

PAGE 340 OF 399 *Total Protein PROTEIN, 2 mL (1 mL min.) serum from 1 SST. Ship Z211 *Albumin *A : Biuret, BCG Daily TOTAL, SERUM refrigerated or frozen. G Ratio

Includes total protein, Capillary PROTEIN electrophoretic 2 mL (1.5 mL min) serum from 1 SST. Ship Electrophoresis, Sample Daily by ELECTROPHORE fractions, Z186 refrigerated or frozen. Grossly hemolysed Agarose Gel 9 am; Report SIS & IFE, densitometric samples not accepted. Immunofixation Next day SERUM tracing of Electrophoresis immunofixation electrophoresis.

For IFE, use test PROTEIN 20 mL (10 mL min.) aliquot of 24 hour urine. Do code E006 Sample Daily by ELECTROPHORE Does not include not use preservatives. Record total volume on Agarose Gel E003 IMMUNO- FIXATION 9 am; Report 4 SIS, 24-HOUR IFE. test request form and urine container. Ship Electrophoresis ELECTROPHORESIS, days URINE refrigerated or frozen. 24-HOUR URINE.

PAGE 341 OF 399 It is recommended that patient 3 mL Whole blood in 1 Blue Top (Sodium discontinues Citrate) tube. Mix thoroughly by inversion. Heparin for 1 day Transport to Lab within 4 hrs. If this is not and Oral possible, make PPP within 1 hour of collection Anticoagulants for 7 as follows: Centrifuge sample at 3600 rpm for days prior to Sample by Thu PROTEIN S, 15 min & transfer supernatent to a clean plastic Electromechanical H180 sampling as these 9 am; Report FUNCTIONAL tube. Centrifuge this supernatant again at 3600 Clot Detection drugs may affect Next day rpm for 15 mins & finally transfer the supernatant test results. (PPP) to 1 labelled, clean plastic screw capped Discontinuation vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT should be with prior THAW. Overnight fasting is preferred. Duly filled consent from the Coagulation Requisition Form is mandatory. treating Physician. Also see DEEÇ

Submit 2 mL (1 mL min.) Synovial fluid in 1 Red Top (No Additive) tube. For other body fluids, PROTEIN, submit 10 mL (5 mL min.) in a screw-capped P089 TOTAL, BODY Biuret Daily container. Specify source of fluid on the test request FLUIDS form and container. Ship refrigerated. DO NOT FREEZE. 20 mL (10 mL min.) aliquot of 24-hour urine. Do PROTEIN, not use preservatives. Record 24 hour volume U004 TOTAL, 24- Spectrophotometry Daily on test request form and urine container. Ship HOUR URINE refrigerated. DO NOT FREEZE. PROTEIN, 15 mL (10 mL min.) aliquot of random urine TOTAL, U020 without preservative. Ship refrigerated. DO NOT Spectrophotometry Daily RANDOM FREEZE. URINE

PAGE 342 OF 399 3 mL Whole blood in 1 Blue Top (Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hrs. If this is not possible, make PPP within 1 hour of collection as follows: Centrifuge sample at 3600 rpm for Sample by 7th PROTEIN C, 15 min & transfer supernatent to a clean plastic of the month; RW157 Immunoassay ANTIGEN tube. Centrifuge this supernatant again at 3600 Report after 2–3 rpm for 15 mins & finally transfer the supernatant weeks (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. Duly filled Coagulation Requisition Form is mandatory.

PROTEIN, 1 mL (0.5 mL min.) Cerebrospinal fluid. Avoid B090 Spectrophotometry Daily TOTAL, CSF hemolysis. Ship refrigerated. PROTHROMBIN Sample by Mon 3 mL (1.5 mL min.) whole blood in 1 Lavender GENE / Thu 11 am; N048 Top (EDTA) tube. Ship refrigerated. DO NOT Real Time PCR MUTATION Report Wed / FREEZE ANALYSIS Sat

3 mL Whole blood in 1 Blue Top (Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hrs. If this is not *Prothrombin possible, make PPP within 1 hour of collection Time (PT) as follows: Centrifuge sample at 3600 rpm for *Prothrombin PROTHROMBIN 15 min & transfer supernatent to a clean plastic Photo-optical Clot H131 Ratio (PR) Daily TIME STUDIES tube. Centrifuge this supernatant again at 3600 Detection *International rpm for 15 mins & finally transfer the supernatant Normalised (PPP) to 1 labelled, clean plastic screw capped Ratio (INR) vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form is mandatory.

PAGE 343 OF 399 2 mL (1 mL min.) serum from 1 SST. Ship PSA (PROSTATE Chemiluminescent refrigerated or frozen. Do not draw sample F017 - SPECIFIC Microparticle Daily within 7 days of Digital Rectal Examination (DRE) ANTIGEN), FREE Immunoassay or Rectal Prostatic Ultrasonography. *PSA, Total 2.5 mL (1.5 mL min.) serum from 1 SST. Ship Chemiluminescent *PSA, Free refrigerated or frozen. Do not draw sample Z262 PSA PROFILE Microparticle Daily *Free/Total within 7 days of Digital Rectal Examination (DRE) Immunoassay Percent or Rectal Prostatic Ultrasonography. PSA (PROSTATE 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent - SPECIFIC refrigerated or frozen. Do not draw sample F014 Microparticle Daily ANTIGEN), within 7 days of Digital Rectal Examination (DRE) Immunoassay TOTAL or Rectal Prostatic Ultrasonography. Simultaneous 3 mL (1.5 mL min.) serum from 1 SST drawn PTH determination of between 8-10 a.m in a fasting condition. Allow (PARATHYROID Serum Total R038 blood to clot. Separate serum and freeze within CLIA Daily HORMONE), Calcium and 30 minutes. Ship frozen. DO NOT THAW. INTACT Phosphorus is Hemolysed samples not acceptable. recommended. PTHrP; 2 mL (1 mL min.) plasma in 1 Green Top (Sodium Sample by 7th PARATHYROID heparin) tube. Separate plasma immediately and of the month; RW160 HORMONE Immunoassay transfer to a screw capped vial. Ship Report after 2–3 RELATED refrigerated or frozen. weeks PEPTIDE

PURKINJE CELL (Yo) ANTIBODY Sample by 7th SCREEN, WITH 3 mL (2 mL min.) CSF in a sterile screw capped Reflex testing if Immunofluorescence of the month; RW161 REFLEX TO container. Ship refrigerated or frozen. Overnight performed shall be Assay Report after 2–3 TITER AND fasting is preferred. charged additionally. weeks WESTERN BLOT, CSF

PAGE 344 OF 399 PURKINJE CELL (Yo) ANTIBODY Sample by 7th SCREEN, WITH 3 mL (2 mL min.) Serum from 1 SST. Ship Reflex testing if Immunofluorescence of the month; RW162 REFLEX TO refrigerated or frozen. Overnight fasting is performed shall be Assay Report after 2–3 TITER AND preferred. charged additionally. weeks WESTERN BLOT, SERUM

PYRIDINIUM 10 mL (5 mL min.) aliquot of a 2-hour urine Sample by 7th COLLAGEN collection. Discard the first morning void. Collect High Performance of the month; RW163 CROSS-LINKS urine for the next two hours, with 2 mL 6N HCl Liquid Report after 2–3 (PYD), 2-HOUR or 1g boric acid. Mix well before aliquoting. Ship Chromatography weeks URINE refrigerated or frozen

3 mL (2 mL min.) whole blood from 1 Grey Top (Sodium Fluoride) tube. Mix whole blood Sample Daily by PYRUVATE; immediately with equal volume of 7% Perchloric G148 Spectrophotometry 4 pm; Report PYRUVIC ACID Acid. Mix well and centrifuge immediately. Same day Separate supernatant and submit minimum 2 mL. Ship refrigerated or frozen. Q FEVER Sample by 7th (COXIELLA 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence of the month; RW165 BURNETTI) refrigerated or frozen. Assay Report after 2–3 ANTIBODIES, weeks IgG & IgM

PAGE 345 OF 399 Collect 1 mL whole blood each in a set of special QuantiFERON – TB Gold tubes available QUANTIFERON- from LPL. Shake the tubes vigorously at least 10 Sample by Mon TB GOLD ; times to ensure thorough mixing. DO NOT Enzyme through Sat 4 B160 GAMMA CENTRIFUGE. Ship at room temperature to reach Immunoassay pm; Report Next INTERFERON lab within 16 hours OR Incubate the tubes Day immediately at 37°C for 16-24 hours and centrifuge. Ship refrigerated. DO NOT FREEZE.

Submit pure growth of AFB on appropriate media in sterile screw capped container in a RAPID TB sealed plasticbag. Ship refrigerated OR when Immunochromatogr M175 ANTIGEN used with other AFB culture or Mycobacteria Daily aphy MPT64 identification tests, the pure growth obtained in the laboratory from specimens already submitted will be processed. RED BLOOD 3 mL (2 mL min.) whole blood in 1 Lavender H004 CELLS; RBC Top (EDTA) tube. Ship refrigerated. DO NOT Electrical Impedence Daily COUNT FREEZE. RED CELL 3 mL (2 mL min.) whole blood in 1 Lavender H019 DISTRIBUTION Top (EDTA) tube. Ship refrigerated. DO NOT Electrical Impedence Daily WIDTH; RDW FREEZE. *Staphylococcus aureus *Streptococcus Submit Throat/nasal swabs in special collection Sample by Mon / RESPIRATORY pneumoniae medium available from LPL OR 5mL (3mL min.) N103 Real Time PCR Thu 11 am; PANEL 6 *Chlamydia Sputum / Bronchoalveolar lavage fluid. Ship Report Wed / Sat pneumoniae refrigerated or frozen. *Haemophilus influenzae Type B

PAGE 346 OF 399 Submit Throat/nasal swabs in special collection *Coronaviruses Sample by Mon / RESPIRATORY medium available from LPL OR 5mL (3mL min.) N100 NL63, 229E, Real Time PCR Thu 11 am; PANEL 3 Sputum / Bronchoalveolar lavage fluid. Ship OC43 & HKU1 Report Wed / Sat refrigerated or frozen. *Respiratory syncytial virus Submit Throat/nasal swabs in special collection A/B Sample by Mon / RESPIRATORY medium available from LPL OR 5mL (3mL min.) N102 *Cytomegaloviru Real Time PCR Thu 11 am; PANEL 5 Sputum / Bronchoalveolar lavage fluid. Ship s *Enterovirus / Report Wed / Sat refrigerated or frozen. Parechovirus *Adenovirus Submit Throat/nasal swabs in special collection RESPIRATORY *Respiratory Sample by Mon / medium available from LPL OR 5mL (3 mL min.) Z815 COMPREHENSIV Panels 1-8 (33 Real Time PCR Thu 11 am; Sputum / Bronchoalveolar lavage fluid. Ship E PANEL Pathogens) Report Wed / Sat refrigerated or frozen. *Parainfluenza Virus 1 *Human Submit Throat/nasal swabs in special collection Metapneumoviru Sample by Mon / RESPIRATORY medium available from LPL OR 5mL (3mL min.) N101 s A/B Real Time PCR Thu 11 am; PANEL 4 Sputum / Bronchoalveolar lavage fluid. Ship *Bocavirus, Report Wed / Sat refrigerated or frozen. *Mycoplasma pneumoniae RESPIRATORY Nasal / Nasopharyngeal / BAL / Respiratory Sample Daily by 1 SYNCYTIAL M205 secretions in a sterile screw capped container. Ship ICT pmReport Same VIRUS (RSV), refrigerated only.DO NOT FREEZE. day ANTIGEN Submit Throat/nasal swabs in special collection Sample by Mon / RESPIRATORY *Influenza A & B medium available from LPL OR 5mL (3mL min.) N098 Real Time PCR Thu 11 am; PANEL 1 *Rhino Virus Sputum / Bronchoalveolar lavage fluid. Ship Report Wed / Sat refrigerated or frozen.

PAGE 347 OF 399 *Moraxella catarrhalis Submit Throat/nasal swabs in special collection *Bordetella Sample by Mon / RESPIRATORY medium available from LPL OR 5mL (3 mL min.) N105 pertussis Real Time PCR Thu 11 am; PANEL 8 Sputum / Bronchoalveolar lavage fluid. Ship *Influenza C Report Wed / Sat refrigerated or frozen. *Haemophilus influenzae *Klebsiella pneumoniae*Leg Submit Throat/nasal swabs in special collection ionella Sample by Mon / RESPIRATORY medium available from LPL OR 5mL (3mL min.) N104 *Pneumocystis Real Time PCR Thu 11 am; PANEL 7 Sputum / Bronchoalveolar lavage fluid. Ship jiroveci Report Wed / Sat refrigerated or frozen. *Salmonella species Submit Throat/nasal swabs in special collection Sample by Mon / RESPIRATORY *Parainfluenza medium available from LPL OR 5mL (3mL min.) N099 Real Time PCR Thu 11 am; PANEL 2 viruses 2, 3 & 4 Sputum / Bronchoalveolar lavage fluid. Ship Report Wed / Sat refrigerated or frozen. Sample by 7th RETICULIN IgA 2 mL (1 mL min.) serum from 1 SST. Ship of the month; RW166 Immunoassay ANTIBODY refrigerated or frozen. Report after 2–3 weeks 3 mL (2 mL min.) whole blood in 1 Lavender RETICULOCYTE H011 Top (EDTA) tube. Ship refrigerated. DO NOT Electrical Impedence Daily COUNT FREEZE. RETT Sample by Mon 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS180 SYNDROME PCR Sequencing 11 am; Report (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. DETECTION 11 days RHEUMATOID Also see 2 mL (1 mL min.) serum from 1 SST. Ship B072 FACTOR (RA), Immunoturbidimetry RHEUMATOID Daily refrigerated or frozen. SERUM FACTOR, IgA.

PAGE 348 OF 399 RHEUMATOID 2 mL (1 mL min.) Synovial Fluid. Ship refrigerated or P070 FACTOR (RA), Immunoturbidimetry Daily frozen. SYNOVIAL FLUID

*ANA *Anti-ds DNA *Sm *U1RNP RHEUMATOID *Rheumatoid 6 mL (4 mL min.) serum from 2 SST’s. Ship Enzyme Also see Sample by Tue / AUTOIMMUNE Z097 Factor *C3 *C4 refrigerated or frozen. Overnight fasting is Immunoassay, RHEUMATOID Fri 9 am; COMPREHENSIV *SS-A (Ro) *SS- preferred. Immunoturbidimetry FACTOR, IgA. Report Same day E PANEL B (La) *Centromere Antibody *ANA *Anti-ds RHEUMATOID DNA *Sm 3 mL (1.5 mL min.) serum from 1 SST. Ship Enzyme Also see Sample by Tue / Z096 AUTOIMMUNE *U1RNP refrigerated or frozen. Overnight fasting is Immunoassay, RHEUMATOID Fri 9 am; PANEL *Rheumatoid preferred. Immunoturbidimetry FACTOR, IgA. Report Same day Factor *C3 *C4 *ANA *Rheumatoid Enzyme Also see Sample Mon RHEUMATOID Factor *Anti 3 mL (1.5 mL min.) serum from 1 SST. Ship Immunoassay, RHEUMATOID through Sat 9 Z143 ARTHRITIS Cyclic refrigerated or frozen. Immunoturbidimetry FACTOR, IgA; am; Report PANEL Citrullinated , CMIA Arthritis Panels Same day Peptide Sample by Sat 9 RHEUMATOID 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S154 am; Report FACTOR, IgA refrigerated or frozen. Immunoassay Same day Sample by 7th RIBOSOME P 2 mL (1 mL min.) serum from 1 SST. Ship of the month; RW167 Immunoassay ANTIBODY, IgG refrigerated or frozen. Report after 2–3 weeks

PAGE 349 OF 399 *Rocky Mountain Sample by 7th RICKETTSIA Spotted Fever 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence of the month; RW168 ANTIBODY IgG & IgM refrigerated or frozen. Assay Report after 2–3 PANEL *Typhus Fever, weeks IgG & IgM

3 mL Whole blood in 1 Blue Top (Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hrs. If this is not possible, make PPP within 1 hour of collection as follows: Centrifuge sample at 3600 rpm for Sample by 7th RISTOCETIN 15 min & transfer supernatent to a clean plastic Platelet of the month; RW169 COFACTOR tube. Centrifuge this supernatant again at 3600 Agglutination Report after 2–3 rpm for 15 mins & finally transfer the supernatant weeks (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen.DO NOT THAW. Overnight fasting is mandatory. Duly filled Coagulation Requisition Form is mandatory.

ROCKY *Rickettsia Sample by 7th MOUNTAIN rickettsii IgG & 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence of the month; RW170 SPOTTED IgM (Both refrigerated or frozen. Assay Report after 2–3 FEVER Screen and weeks ANTIBODIES Titer) ROMA; RISK OF *CA125 *HE4 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent Sample by Mon OVARIAN *Menopausal Z673 refrigerated or frozen. Provide menopausal status Microparticle / Thu 4 pm; MALIGNANCY status risk of the patient. Immunoassay Report Same day ALGORITHM estimation ROTAVIRUS Sample Daily by 1 5 g fresh STOOL in a sterile screw capped container. M212 ANTIGEN, ICT pm Report Same Ship refrigerated only. DO NOT FREEZE. STOOL day

PAGE 350 OF 399 RUBELLA (GERMAN 2 mL (1 mL min.) serum from 1 SST. Ship S040 CLIA Daily MEASLES) refrigerated or frozen. ANTIBODY, IgG RUBELLA Sample Daily 9 (GERMAN 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme B158 am. Report MEASLES) refrigerated or frozen. Immunoassay Same day. AVIDITY, IgG RUBELLA (GERMAN MEASLES) 2 mL (1 mL min.) serum from 1 SST. Ship Z190 CLIA Daily ANTIBODIES refrigerated or frozen. PANEL, IgG & IgM RUBELLA (GERMAN 2 mL (1 mL min.) serum from 1 SST. Ship S016 CLIA Daily MEASLES) refrigerated or frozen. ANTIBODY, IgM

4 mL (2 mL min.) Plasma from 1 Lavendar top RUBELLA Sample by (EDTA) tube OR 3 mL (2 mL min.) CSF/Amniotic fluid OS181 DETECTION , PCR Wed/Sat 11 am; in a sterile screw capped container OR PCR Report Sat/Tue Nasopharyngeal swab. Ship refrigerated or frozen. SCA-6 (SPINO CEREBELLAR ATAXIA) Sample by Tue 11 4 mL (2 mL min.) Whole Blood in 1 Lavender top PCR Fragment OS185 DETECTION , am; Report 10 (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. analysis PCR days FRAGMENT ANALYSIS

PAGE 351 OF 399 SCA-3 (SPINO CEREBELLAR ATAXIA) Sample by Tue 11 4 mL (2 mL min.) Whole Blood in 1 Lavender top PCR Fragment OS184 DETECTION , am; Report 10 (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. analysis PCR days FRAGMENT ANALYSIS SCA-1 (SPINO CEREBELLAR ATAXIA) Sample by Tue 11 MUTATION 4 mL (2 mL min.) Whole Blood in 1 Lavender top PCR Fragment OS182 am; Report 10 DETECTION , (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. analysis days PCR FRAGMENT ANALYSIS SCA-2 (SPINO CEREBELLAR ATAXIA) Sample by Tue 11 4 mL (2 mL min.) Whole Blood in 1 Lavender top PCR Fragment OS183 DETECTION , am; Report 10 (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. analysis PCR days FRAGMENT ANALYSIS SCA-7 (SPINO CEREBELLAR ATAXIA) Sample by Tue 11 4 mL (2 mL min.) Whole Blood in 1 Lavender top PCR Fragment OS186 DETECTION , am; Report 10 (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. analysis PCR days FRAGMENT ANALYSIS *Scl - 70 Sample by Tue / SCLERODERMA 3 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z192 *Centromere Fri 9 am; Report PANEL refrigerated or frozen. Immunoassay Antibody Same day

PAGE 352 OF 399 Scl-70 Also see Sample by Tue / 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S065 (SCLERODERMA EXTRACTABLE Fri 9 am; Report refrigerated or frozen. Immunoassay ) ANTIBODY NUCLEAR ANTIGENS. Same day

SCRUB TYPHUS Sample Daily by 1 2 mL (1 mL min.) serum from 1 SST. Ship Immunochromatogr V086 (TSUTSUGAMUS pm; Report Same refrigerated or frozen. aphy HI) ANTIBODY day

Use only specified containers avalable 10 mL (5 mL min.) aliquot of 24 hour urine from LPL. If collected in an acid washed (metal free) container gadolinium, iodine kit available from LPL. Mix thoroughly. Transfer 10 or barium Sample Daily by SELENIUM, 24- C048 mL aliquot to vial provided in the kit. Measure ICPMS containing contrast 6 pm; Report HOUR URINE 24 hour total volume and record on vial and media has been Next day test request form. Ship refrigerated or frozen. administered, the Use powderless gloves during specimen collection. specimen should not be collected for 96 hours.

PAGE 353 OF 399 Use only specified containers avalable from LPL.If gadolinium, iodine 10 mL (5 mL min.) aliquot of random urine SELENIUM, or barium Sample Daily by collected in an acid washed (metal free) container C047 RANDOM ICPMS containing contrast 6 pm; Report kit available from LPL. Ship refrigerated or frozen. URINE media has been Next day Use powderless gloves during specimen collection. administered, the specimen should not be collected for 96 hours.

Use only specified containers avalable from LPL. If gadolinium, iodine 2 mL (1.0 mL min.) serum from 1 metal free White or barium Sample Daily by SELENIUM, Top (Z-No Additive) tube available from LPL. Ship C032 ICPMS containing contrast 6 pm; Report SERUM refrigerated or frozen. Use powderless gloves media has been next day during specimen collection. administered, the specimen should not be collected for 96 hours.

PAGE 354 OF 399 Submit Semen in a sterile screw capped container. Specimen must reach the Lab within 1 hour of collection. Condoms are not acceptable. Print exact time of collection on specimen Outstation samples container and test request form. Patient should not accepted. Use SEMEN refrain from ejaculation for 2-7 days (ideally 3 Physical test code P022 for Sample Daily by P006 ANALYSIS; days) prior to collection. Specimen must be Examination, SEMEN FRUCTOSE, 1 pm; Report SEMINOGRAM obtained by masturbation only. Immediately prior Microscopy QUANTITATIVE Same day to collection, patient should urinate, wash available as a genital area with warm water and dry the part. separate test. Collect the entire ejaculate. Specimen must be kept at body temperature (shirt pocket- 37°C) after collection.

SEMEN Submit semen in a sterile screw capped P022 FRUCTOSE, container. Freeze immediately after collection. Ship Biochemical analysis Daily QUALITATIVE frozen. DO NOT THAW.

PAGE 355 OF 399 Systemic inflammatory response syndrome 5 mL ( 3 mL min.) serum from 1 SST AND 3 mL (1 mL (SIRS) may have an min.) whole blood from 1 Lavender Top (EDTA) tube ECLIA, infectious or non- AND 2 mL (1mL min.) plasma from 1 Grey Top *Procalcitonin * Spectrophotometry, infectious etiology. (Sodium Fluoride ) tube. Separate plasma Sample Daily by Lactate * C- Immunoturbidimetry If infection is immediately AND Collect 8-10 mL blood aseptically 3.30 pm; Report Z804 SEPSIS PANEL Reactive Protein , Rapid Automated suspected or in special Plus Aerobic bottle available from LPL. For Interim: 24 & 48 *Blood culture Aerobic culture, proven, patients children less than 12 yrs collect 2 mL blood in hrsFinal: 6 days *CBC Electrical with SIRS are said to Special Paeds Plus bottle available from LPL. Mix by Impedence, VCS have sepsis. gentle swirling. Ship refrigerated only. Do not use Microbial invasion of tourniquet while drawing samples. the blood stream is not essential since local inflammaÇ

3 mL (1.5 mL min) serum from 1 SST. Separate serum within 2 hours of collection in a plastic transport vial and freeze immediately. Ship frozen. Sample by 7th SEROTONIN (5 - DO NOT THAW. Avoid food rich in indoles like HPLC, Fluorescence of the month; RW173 HT), SERUM avocado, banana, tomato, plum, walnut, detection Report after 2–3 pineapple, egg plant (brinjal) and also avoid weeks tobacco, tea and coffee 3 days prior to specimen collection. SERUM ASCITES 2 mL (0.5 mL min.) serum from 1 SST. AND 2 mL (1 B140 ALBUMIN mL min.) Ascitic fluid in a sterile screw capped BCG Daily GRADIENT container. Ship refrigerated or frozen. (SAAG)

PAGE 356 OF 399 SEX HORMONE 2 mL (1 mL min.) serum from 1 SST. Specify age Sample by Mon BINDING Chemiluminescent R070 and sex on test request form. Ship refrigerated or / Thu 4 pm; GLOBULIN; Immunoassay frozen. Report same day SHBG SGOT; 2 mL (1 mL min.) serum from 1 SST. Ship ASPARTATE B032 refrigerated or frozen. Hemolysed specimens are IFCC Daily AMINOTRANSFE not acceptable. RASE (AST) SGPT; ALANINE 2 mL (1 mL min.) serum from 1 SST. Ship B033 AMINOTRANSFE refrigerated or frozen. Hemolysed specimens are IFCC Daily RASE (ALT) not acceptable.

4 mL (2 mL min.) Whole Blood in 1 Lavender top SICKLE CELL (EDTA) Tube from both parents AND 10 mL (5 mL Sample by Tue 11 MUTATION min.) Amniotic fluid in a sterile screw capped OS188 PCR am; Report 10 DETECTION , container. Ship refrigerated. DO NOT FREEZE.It is days PRENATAL mandatory to send a Signed Prenatal Consent Form with the specimen.

The recommended SICKLING, 2 mL (1 mL min.) whole blood in 1 Lavender Sample Daily by test is H042 SCREENING Top (EDTA) tube. Ship refrigerated. DO NOT Deoxygenation 9 am; Report HEMOGLOBIN HPLC TEST FREEZE. Next day / ELECTROPHORESIS.

SICKLE CELL Sample by Tue 11 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS187 MUTATION PCR am; Report 10 (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. DETECTION days Also see AMINO SILVER Sample Daily by 10 mL (5 mL min.) aliquot of random urine. Ship ACID PANELS, G144 NITROPRUSSIDE Chemical 4 pm; Report refrigerated or frozen. QUALITATIVE AND TEST, URINE Same day QUANTITATIVE

PAGE 357 OF 399 Use only specified containers avalable from LPL. If 3 ml (1 ml min) serum from 1 metal free White Top gadolinium or (Z tube- No Additive) tube available from LPL. Ship iodine containing Sample Tue / Fri refrigerated or frozen. Patient should avoid silver C066 SILVER, SERUM ICPMS contrast media has by 6 pm; containing medication or food-supplements 3 days been administered, Report 2 days prior to sample collection. Use powderless gloves the specimen during specimen collection. should not be collected for 96 hours.

3 mL (2 mL min.) Whole blood in 1 Lavender Sample Daily by SIROLIMUS G162 Top (EDTA) tube. Ship refrigerated . Ideal Sampling LC-MS/MS 5 pm: Report (RAPAMYCIN) time: 1 hour before next dose (12 hour trough) Same day

SJOGREN’S Also see Sample by Tue / *SS-A/Ro *SS- 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z142 SYNDROME EXTRACTABLE Fri 9 am; Report B/La refrigerated or frozen. Immunoassay ANTIBODIES NUCLEAR ANTIGENS. Same Day *ANA * ds-DNA Antibody * Sm SLE (SYSTEMIC antibody * Sample by Tue / LUPUS 4 mL (2 mL min. ) serum from 1 SST. Ship Z814 Phospholipid EIA Fri 9 am; Report ERYTHEMATOS refrigerated or frozen. antibody IgG * Same Day US) PANEL Phospholipid antibody IgM

SMEAR Submit in a slide mailer, 2 air dried smears fixed Also see CULTURE, P028 EXAMINATION, in 95% ethanol for 1 minute. Ship at room Gram Stain Daily GONOCOCCUS. GONOCOCCUS temperature.

PAGE 358 OF 399 Does not include Papanicoloau Stain, Submit 2 air dried Nasal/Cervical/ ZN Stain and SMEAR Vaginal/Throat/Pus/Other Smears in a slide Light microscopy, Auramine Stain. For P018 EXAMINATION, mailer. Ship at room temperature. Specify nature Daily Giemsa stain AFB stain use test ROUTINE of specimen on Test Request Form and code P030, AFB container. STAIN, MISCELLANEOUS. SMOOTH 2 mL (1 mL min.) serum from 1 SST. Ship For SERIAL Sample Daily by MUSCLE Immunofluorescence S045 refrigerated or frozen. Overnight fasting is DILUTIONS use test 11 am; Report ANTIBODY Assay preferred. code S084. Same day (ASMA), IFA SMOOTH MUSCLE 3 mL (1.5 mL min.) serum from 1 SST. Ship Sample Daily by Immunofluorescence S084 ANTIBODY refrigerated or frozen. Overnight fasting is 11 am; Report Assay (ASMA), IFA IN preferred. Next day DILUTIONS Also see Sample by Tue / Sm (SMITH) 2 mL (1 ml min.) serum from 1 SST. Ship Enzyme S038 EXTRACTABLE Fri 9 am; Report ANTIBODY, IgG refrigerated or frozen. Immunoassay NUCLEAR ANTIGENS. Same day 10 mL (5 mL min.) aliquot of 24-hour urine. Do not SODIUM, 24- use preservatives. Record 24 hour volume on Indirect Ion U025 Daily HOUR URINE container and test request form. Ship Selective Electrode refrigerated. DO NOT FREEZE. SODIUM, 2 mL (1 mL min.) serum from 1 SST. Ship Indirect Ion B044 Daily SERUM refrigerated or frozen. Selective Electrode Indirect ISE, SODIUM : 2 mL (1.5 mL min.) serum from 1 SST. Separate Freezing Point B183 OSMOLALITY serum within 1 hour of collection. Ship Daily Depression, RATIO, SERUM refrigerated or frozen. Calculated

PAGE 359 OF 399 2 mL (1 mL min.) fluid in a sterile screw capped container. Ship refrigerated. DO NOT FREEZE. Indirect Ion P079 SODIUM, FLUID Daily Blood contaminated and highly turbid samples Selective Electrode not acceptable SODIUM, 10 mL (5 mL min.) aliqout of random urine. Do Indirect Ion U013 RANDOM not use preservatives. Ship refrigerated. DO NOT Daily Selective Electrode URINE FREEZE. 2 ml (1 mL min.) plasma from 1 Lavender Top Sample by 7th SOMATOSTATIN (EDTA) tube. Draw blood in prechilled tube, mix Extraction, of the month; RW174 , PLASMA by inversion and separate plasma. Freeze Immunoassay Report after 2–3 immediately. Ship frozen. DO NOT THAW. weeks

SPECIFIC 10 mL (5 mL min.) aliquot of random OR 24 hour Outstation Automated Strip U018 GRAVITY, urine in a sterile screw capped container. Ship specimens not Daily Test URINE refrigerated. DO NOT FREEZE. acceptable

*Metachromatic Leucodystrophy *GM 1 Gangliosidosis 10 mL (7.5 mL min.) whole blood from 3 Lavender Sample Daily by SPHINGOLIPIDO *GM 2 Top (EDTA) / Green Top (Sodium Heparin) tubes. G130 Enzyme assay 4 pm; Report 4 SIS PANEL 1 Gangliosidosis Ship refrigerated within 48 hrs. DO NOT FREEZE. days *Gaucher Clinical history must accompany sample. Disease *Niemann Pick Disease *GM 1 Gangliosidosis 10 mL (7.5 mL min.) whole blood from 3 Lavender Sample Daily by SPHINGOLIPIDO *Gaucher Top (EDTA) / Green Top (Sodium Heparin) tubes. G132 Enzyme assay 4 pm; Report 4 SIS PANEL 3 Disease Ship refrigerated within 48 hrs. DO NOT FREEZE. days *Niemann Pick Clinical history must accompany sample. Disease

PAGE 360 OF 399 *Gaucher 10 mL (7.5 mL min.) whole blood from 3 Lavender Sample Daily by SPHINGOLIPIDO Disease Top (EDTA) / Green Top (Sodium Heparin) tubes. G131 Enzyme assay 4 pm; Report 4 SIS PANEL 2 *Niemann Pick Ship refrigerated within 48 hrs. DO NOT FREEZE. days Disease Clinical history must accompany sample.

SPINAL MUSCULAR 4mL (2 mL min.) Whole Blood in 1 Lavender top Sample Mon by N120 ATROPHY MLPA (EDTA) tube. Ship referigerated. DO NOT FREEZE. 11 am; Report Sat (SMA), CARRIER DETECTION SPINAL MUSCULAR ATROPHY 4mL (2 mL min.) Whole Blood in 1 Lavender top Sample Mon by N119 MLPA (SMA), (EDTA) tube. Ship referigerated. DO NOT FREEZE. 11 am; Report Sat MUTATION DETECTION Includes SPUTUM Fluorescent and Sample Daily by Auramine and Submit Sputum in a sterile screw capped P027 EXAMINATION, conventional 3.30 pm; Report Ziehl Neelsen container. Ship refrigerated. AFB microscopy Same day stain. SPUTUM EXAMINATION, Submit Sputum in a sterile screw capped P067 Light microscopy Daily OVA & container. Ship refrigerated. PARASITES Submit Sputum in a sterile screw capped SPUTUM FOR container OR smeared onto 4 sterile glass slides, P066 Light microscopy Daily EOSINOPHILS air dried and shipped in a slide mailer. Ship refrigerated.

PAGE 361 OF 399 Does not include Gram, Ziehl Neelsen or SPUTUM Submit Sputum in a sterile screw capped Giemsa stain, Light Auramine Stains. P016 EXAMINATION, container OR 2 air dried smears in a slide Daily microscopy For AFB stain use ROUTINE mailer. Ship refrigerated. test code P027 SPUTUM EXAMINATION, AFB.

Also see EXTRACTABLE 2 mL (1 mL min.) serum from 1 SST. Ship Sample by Tue / SS-A/Ro Enzyme NUCLEAR ANTIGENS S066 refrigerated or frozen. Overnight fasting is Fri 9 am; ANTIBODY Immunoassay & SJOGREN’S preferred. Report Same day SYNDROME ANTIBODIES.

Also see EXTRACTABLE 2 mL (1 mL min.) serum from 1 SST. Ship Sample by Tue / SS-B/La Enzyme NUCLEAR ANTIGENS S067 refrigerated or frozen. Overnight fasting is Fri 9 am; ANTIBODY Immunoassay & SJOGREN’S preferred. Report Same day SYNDROME ANTIBODIES.

*VDRL *HIV 1 & 2, Antibodies Screening test Slide flocculation, Sample by Fri 9 *HSV 1 & 2, 5 mL (3 mL min.) serum from 1 SST. Ship Z314 STD PANEL CMIA, EIA, am; Report Next IgG & IgM refrigerated or frozen. day *Chlamydia trachomatis IgG Antibody *TPHA

PAGE 362 OF 399 *Androstenedion e *Cortisol *11- STEROID PANEL 2 mL (1 mL min) serum from 1 Red Top (No Deoxycortisol * FOR Additive) tube. Ship refrigerated or frozen. Diurnal DHEA *17-α- Sample Daily by 4 CONGENITAL variation present; take sample between 8-10 am G191 Hydroxyprogeste LC-MS/MS pm; Report 2 ADRENAL OR 4-6 pm. Specify time and date on specimen rone days HYPERPLASIA container and test request form. Ship refrigerated *Progesterone (CAH) or frozen. *Testosterone, Total

*Androstenedion 2 mL (1 mL min) serum from 1 Red Top (No e * DHEA * STEROID PANEL Additive) tube. Ship refrigerated or frozen. Diurnal DHEAS *17-α- Sample Daily by 4 FOR variation present; take sample between 8-10 am G194 Hydroxy- LC-MS/MS pm; Report 2 PREMATURE OR 4-6 pm. Specify time and date on specimen progesterone days ADRENARCHE container and test request form. Ship refrigerated *Testosterone, or frozen. Total *Estradiol

*17 STEROID Hydroxyprogeste 2 mL (1 mL min) serum from 1 Red Top (No PANEL, 21- rone Additive) tube. Ship refrigerated or frozen. Diurnal HYDROXYLASE *Androstenedion Sample Daily by 4 variation present; take sample between 8-10 am G190 DEFICIENCY/STR e *Cortisol *17- LC-MS / MS pm; Report 2 OR 4-6 pm. Specify time and date on specimen ESS Hydroxyprogeste days container and test request form. Ship refrigerated DIFERENTIATIO rone + or frozen. N Androstenedione : Cortisol Ratio

PAGE 363 OF 399 STEROID PANEL 2 mL (1 mL min) serum from 1 Red Top (No FOR *Androstenedion Additive) tube. Ship refrigerated or frozen. Diurnal Sample Daily by 4 POLYCYSTIC e *DHEA variation present; take sample between 8-10 am G192 LC-MS/MS pm; Report 2 OVARY *Testosterone, OR 4-6 pm. Specify time and date on specimen days SYNDROME Total container and test request form. Ship refrigerated (PCOS) or frozen.

* Androstenedione *DHEA *DHEAS 2 mL (1 mL min) serum from 1 Red Top (No *Dihydrotestoste Additive) tube. Ship refrigerated or frozen. Diurnal rone (DHT) Sample Daily by 4 STEROID PANEL variation present; take sample between 8-10 am G196 *Estradiol *17-α- LC-MS/MS pm; Report 2 2, 8 STEROIDS OR 4-6 pm. Specify time and date on specimen Hydroxyprogeste days container and test request form. Ship refrigerated rone * or frozen. Progesterone *Testosterone, Total

PAGE 364 OF 399 *Aldosterone *Androstenedion e *Corticosterone *Cortisol *Cortisone *11- 2 mL (1 mL min) serum from 1 Red Top (No Deoxycortisol Additive) tube. Ship refrigerated or frozen. Diurnal Sample Daily by 4 STEROID PANEL *DHEA *DHEAS variation present; take sample between 8-10 am G197 LC-MS/MS pm; Report 2 3, 13 STEROIDS *Dihydrotesteron OR 4-6 pm. Specify time and date on specimen days e (DHT) * container and test request form. Ship refrigerated Estradiol *17α- or frozen. Hydroxyprogeste rone *Progesterone *Testosterone, Total *Androstenedion e *11- 2 mL (1 mL min) serum from 1 Red Top (No STEROID PANEL Deoxycortisol * Additive) tube. Ship refrigerated or frozen. Diurnal FOR PCOS / Sample Daily by 4 DHEA *17-α- variation present; take sample between 8-10 am G193 CAH LC-MS/MS pm; Report 2 Hydroxyprogeste OR 4-6 pm. Specify time and date on specimen DIFFERENTIATIO days rone * container and test request form. Ship refrigerated N Testosterone, or frozen. Total 2 mL (1 mL min) serum from 1 Red Top (No *Aldosterone Additive) tube. Ship refrigerated or frozen. Diurnal *Corticosterone Sample Daily by 4 STEROID PANEL variation present; take sample between 8-10 am G195 *Cortisol LC-MS/MS pm; Report 2 1, 5 STEROIDS OR 4-6 pm. Specify time and date on specimen *Cortisone*11- days container and test request form. Ship refrigerated Deoxycortisol or frozen.

PAGE 365 OF 399 STONE Submit stone (s) in a clean screw capped Use this test code Sample Daily by FTIR, Digital C031 ANALYSIS WITH container. Ship at room temperature or for both Kidney & 9 am; Report Photography PICTURE refrigerated. Gall stones. Next day STOOL EXAMINATION, 5 mL (3 mL min.) stool in a leak-proof screw Sample Daily by Also see CULTURE, T013 HANGING capped container. Specimen must reach LPL Light microscopy 5.30 pm; Report CHOLERA. DROP within 1 hour of collection at room temperature. 2 hours PREPARATION STOOL 5g (3g min.) stool in a leak-proof screw capped EXAMINATION, container. Do not send stool specimens in match T001 Light microscopy Daily ROUTINE; boxes or absorbant material. Outstation samples STOOL, R/E not accepted. 5g (3g min.) stool in a leak-proof screw capped container. Do not use match box or absorbant STOOL material. Patient should avoid meat products, T002 EXAMINATION, pain killers, iron tablets, Vitamin C (Ascorbic Acid) Standard Guaiac Daily OCCULT BLOOD and hard tooth brush for 3 days prior to specimen collection. Outstation specimens not accepted. STOOL EXAMINATION, 5g (3g min.) stool in a leak-proof screw capped pH Strip test, T010 pH & container. Do not use matchbox or absorbant Daily Benedict’s Test REDUCING material. Outstation samples not accepted. SUBSTANCES STREPTOCOCCU 2 mL (1 mL min.) serum from 1 SST OR 2 mL (1 mL Also see S GROUP B min.) Cerebrospinal fluid OR 10 mL (5 mL min.) ANTISTREPTOLYSIN - S149 Latex Agglutination Daily ANTIGEN aliquot of random urine. Ship refrigerated or O, ANTI DNase B, DETECTION frozen. and STREPTOZYME.

PAGE 366 OF 399 Also see ANTISTREPTOLYSIN - Sample by 7th O, ANTI DNase B, 2 mL (1 mL min.) serum from 1 SST. Ship Slide of the month; RW175 STREPTOZYME and refrigerated or frozen. Hemagglutination Report after 2–3 STREPTOCOCCUS weeks GROUP B ANTIGEN DETECTION.

STRIATED / 2 mL (1 mL min.) serum from 1 SST. Ship Sample by Sat / SKELETAL Immunofluorescence S100 refrigerated or frozen. Overnight fasting is Tue 9 am; Report MUSCLE Assay preferred. 4 days ANTIBODY, IFA

STRIATED / SKELETAL 2 mL (1 mL min.) serum from 1 SST. Ship Sample by Sat / Immunofluorescence S174 MUSCLE refrigerated or frozen. Overnight fasting is Tue 9 am; Report Assay ANTIBODY, IFA preferred. 4 days IN DILUTIONS 10 mL (5 mL min.) aliqout of random urine in a Gas Sample Daily by SUCCINYLACET sterile screw capped container. Do not use G171 Chromatography / 5 pm; Report 5 ONE, URINE preservatives. Ship refrigerated or frozen. Provide Mass Spectrometry days brief clinical history Sample by Mon 1 drop of finger /heel prick fresh blood on special SUCCINYLACET / Wed / Fri 9 G163 filter paper available from LPL. Ship refrigerated. DO LC-MS/MS ONE, BLOOD am; Report Wed NOT FREEZE. / Fri / Mon

3 mL (2 mL min.) whole blood from 1 Lavender Top SUDAN BLACK (EDTA) tube OR 2 mL (1 mL min.) Bone Marrow Sample Daily by B STAIN, Cytochemical H226 from 1 Green Top (Sodium heparin) tube. Ship 6 pm; Report 3 BLOOD / BONE staining refrigerated. DO NOT FREEZE. Clinical history days MARROW mandatory.

PAGE 367 OF 399 5g (3g min.) Stool OR 10 mL (5 mL min.) Urine OR 5 Sample Daily by Special stain, J058 SUDAN IV STAIN mL (2 mL min.) Body Fluids in a screw capped 1 pm; Report Microscopy container. Ship refrigerated. DO NOT FREEZE. Next day

Sample by SYPHILIS 2 mL (1 mL min.) serum from 1 SST. Ship S252 CLIA Mon/Thu 9 am; SEROLOGY refrigerated or frozen. Report Same day

3 mL (2 mL min.) Whole blood in 1 Lavender Top (EDTA) tube. Ship refrigerated . Ideal Sampling Sample Daily by TACROLIMUS; G160 Time: Just before the next dose (Trough levels). LC-MS/MS 5 pm; Report FK506 Provide date and time of sampling, amount & Same day time of dose.

TACROLIMUS Sample by 3 ml (2 ml min.) whole blood in 1 Lavender top N111 GENOTYPING Real Time PCR Mon/Thu 11 am; (EDTA) tube. Ship refrigerated. DO NOT FREEZE. (CYP3A5) Report Wed / Sat Sample by 7th 2 mL (1 mL min.) serum from 1 Red Top (No of the month; RW178 T4 ANTIBODY Radiobinding Assay Additive) tube. Ship refrigerated or frozen. Report after 2–3 weeks

PAGE 368 OF 399 Nonthyroidal chronic diseases, nicotinic acid and nutrition may decrease results. Thyrotoxicosis may Sample by 7th 2 mL (1 mL min.) serum from 1 SST. Ship not always cause of the month; RW176 T3 ANTIBODY Immunoassay refrigerated or frozen. abnormal results. Report after 2–3 Binding protein weeks variations may cause variations in results. Increases may be associated with prÇ

TBG; Sample by 7th THYROXINE 2 mL (1 mL min.) serum from 1 SST. Ship of the month; RW179 Chemiluminescence BINDING refrigerated or frozen. Report after 2–3 GLOBULIN weeks TBII; THYROTROPIN - Sample by 7th BINDING 2 mL (1 mL min.) serum from 1 SST. Ship of the month; RW180 Radioreceptor Assay INHIBITORY refrigerated or frozen. Report after 2–3 IMMUNOGLOB weeks ULIN T-CELL GENE REARRANGEME 4 mL (2 mL min.) Whole Blood in 1 Lavender top Sample by Sat 11 OS193 PCR NT MUTATION (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. am; Report 9 days DETECTION

PAGE 369 OF 399 TESTOSTERONE Sample by 7th FREE, 3 mL (2 mL min.) serum from 1 Red Top (No LC-MS/MS, of the month; RW182 BIOAVAILABLE Additive) tube. Ship refrigerated or frozen. Spectrophotometry Report after 2–3 & TOTAL weeks Sample by Mon TESTOSTERONE 3 mL (2 mL min.) serum from 1 SST. Ship Radioimmunoassay, through Sat 9 Z384 PANEL, TOTAL refrigerated or frozen. Specify age and sex on Chemiluminescent am; Report & FREE test request form. Immunoassay Same day Test performed is 2 mL (1 mL min.) serum from 1 SST for each timed Testosterone specimen. Draw fasting baseline specimen on Total.Refer to Y TESTOSTERONE day 1; administer HCG, 5000 IU IM. Draw second Codes:Y114 Basal Chemiluminescent Z083 STIMULATION specimen for Testosterone after 72 hours. Ship Y115 Post HCG 72 Daily Immunoassay BY HCG refrigerated or frozen. Both containers must be hoursY116 clearly marked with time and date drawn, and CustomizedInjection submitted with 1 test request form. HCG is not supplied by LPL. TESTOSTERONE, FREE & SEX 3 mL (1.5 mL min.) serum from 1 SST. Ship Sample by Mon Radioimmunoassay, Z196 HORMONE refrigerated or frozen. Specify age and sex on / Thu 9 am; CLIA BINDING test request form. Report Same Day GLOBULIN 2 mL (1 mL min.) serum from 1 SST. Ship TESTOSTERONE, Chemiluminescent R010 refrigerated or frozen. Specify age and sex on Daily TOTAL Immunoassay test request form. Sample by Mon 3 mL (1 mL min.) serum from 1 SST. Ship TESTOSTERONE, through Sat 9 R009 refrigerated or frozen. Specify age and sex on Radioimmunoassay FREE am; Report test request form. Same day

PAGE 370 OF 399 Sample by 7th TETANUS 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme of the month; RW183 ANTITOXID refrigerated or frozen. Immunoassay Report after 2–3 weeks This is the recommended test for T3 assay as it 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent R090 T3, FREE; FT3 detects the Daily refrigerated or frozen. Immunoassay biologically active fraction in circulating blood. This is the recommended test for T4 assay as it 2 mL (1 ml min.) serum from 1 SST. Ship Chemiluminescent R091 T4, FREE; FT4 detects the Daily refrigerated or frozen. Immunoassay biologically active fraction in circulating blood. T4, FREE & 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent Z194 T3, FREE; FT4 Daily refrigerated or frozen. Immunoassay & FT3 T4, FREE & TSH 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent Z195 Daily ULTRASENSITIV refrigerated or frozen. Immunoassay E THALASSEMIA Sample by Mon BETA, 4 mL (2 mL min.) whole blood from 1 Lavender Top PCR, Reverse N093 11 am; Report MUTATION (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Hybridization Saturday ANALYSIS

PAGE 371 OF 399 HPLC is the method of choice. Hemoglobin electrophoresis *Iron Studies (Alkaline pH) on *Complete Spectrophotometry, 2 mL (1 mL min.) serum from 1 SST AND 5 mL (3.5 cellulose acetate Sample Daily by THALASSEMIA Blood Count Electrical Z049 mL min.) whole blood from 2 Lavender Top will be carried out, 9 am; Report PROFILE *Hemoglobin Impedence, VCS & (EDTA) tubes. Ship refrigerated. DO NOT FREEZE. if necessary, in Next day HPLC/Electropho HPLC selected cases, at resis no additional charge and reported after 3 days.

Use only specified 10 mL (5 mL min.) aliquot of 24 hour urine containers available collected in an acid washed (metal free) from LPL. If container kit available from LPL. Mix thoroughly. gadolinium or iodine Sample Daily by THALLIUM, 24 Transfer 10 mL aliquot to vial provided in the containing contrast C043 ICPMS 6 pm; Report HOUR URINE kit. Measure 24 hour total volume and record media has been next day on vial and test request form. Ship refrigerated administered, the or frozen. Use powderless gloves during specimen specimen should collection. not be collected for 96 hours.

PAGE 372 OF 399 Use only specified containers available from LPL. If 4 mL (2 mL min.) whole blood in 1 metal free gadolinium or iodine Sample Daily by THALLIUM, Royal Blue Top (K2EDTA) tube available from LPL. containing contrast C042 ICPMS 6 pm; Report BLOOD Ship refrigerated. DO NOT FREEZE. Use powderless media has been Next day gloves during specimen collection. administered, the specimen should not be collected for 96 hours.

Use only specified containers available from LPL. If 10 mL (5 mL min.) aliquot of random urine in an gadolinium or iodine THALLIUM, Sample Daily by acid washed (metal free) container available from containing contrast C044 RANDOM ICPMS 6 pm; Report LPL. Ship refrigerated or frozen. Use powderless media has been URINE Next day gloves during specimen collection. administered, the specimen should not be collected for 96 hours.

PAGE 373 OF 399 Does not include Papanicoloau Stain, Submit throat swab obtained by rubbing over ZN Stain and THROAT SWAB, affected / localized lesion in aerobic transport Light microscopy, Auramine Stain. For P017 Daily ROUTINE medium available from LPL AND 2 air dried Giemsa stain AFB stain use test smears on sterile glass slides. Ship refrigerated code P030, AFB STAIN, MISCELLANEOUS.

3 mL Whole blood in 1 Blue Top (Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hrs. If this is not possible, make PPP within 1 hour of collection as follows: Centrifuge sample at 3600 rpm for THROMBIN 15 min & transfer supernatent to a clean plastic Electromechanical H038 Daily TIME; TT tube. Centrifuge this supernatant again at 3600 Clot Detection rpm for 15 mins & finally transfer the supernatant (PPP) to 1 labelled, clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen.DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form is mandatory.

PAGE 374 OF 399 *Protein C, 6 mL Whole blood in 2 Blue Top (Sodium It is recommended Functional Citrate) tubes. Mix thoroughly by inversion. that patient *Protein S, Transport to Lab within 4 hrs. If this is not discontinues Functional possible, make PPP within 1 hour of collection Heparin for 1 day *Antithrombin as follows: Centrifuge sample at 3600 rpm for and Oral THROMBOPHILI Activity 15 min & transfer supernatent to a clean plastic Anticoagulants for 7 Electromechanical & Sample by Wed A Functional tube. Centrifuge this supernatant again at 3600 days prior to Z497 Photo-optical Clot 6 pm; Report COMPREHENSIV *Factor V rpm for 15 mins & finally transfer the supernatant sampling as these Detection, PCR on Sat E PROFILE Leiden Mutation (PPP) to 1 labelled, clean plastic screw capped drugs may affect *Prothrombin vial. FREEZE IMMEDIATELY. Ship frozen.DO NOT test results. Gene Mutation THAW AND 5 mL (3 mL min.) whole blood in 2 Discontinuation *MTHFR gene Lavender Top (EDTA) tubes. Ship refrigerated. DO should be with prior mutation *Lupus NOT FREEZE. Overnight fasting is preferred. Duly consent from the anticoagulant filled Coagulation Requisition Form is mandatory. treating Physician.

3 mL Whole blood in 1 Blue Top (Sodium It is recommended *Protein C, Citrate) tube. Mix thoroughly by inversion. that patient Functional Transport to Lab within 4 hrs. If this is not discontinues *Protein S, possible, make PPP within 1 hour of collection Heparin for 1 day Functional as follows: Centrifuge sample at 3600 rpm for and Oral *Antithrombin 15 min & transfer supernatent to a clean plastic Anticoagulants for 7 Activity Electromechanical & Sample by Wed THROMBOPHILI tube. Centrifuge this supernatant again at 3600 days prior to Z319 Functional Photo-optical Clot 6 pm; Report A PROFILE rpm for 15 mins & finally transfer the supernatant sampling as these *Factor V Detection, PCR on Sat (PPP) to 1 labelled, clean plastic screw capped drugs may affect Leiden Mutation vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT test results. *Prothrombin THAW AND 5 mL (3 mL min.) whole blood in 2 Discontinuation Gene Mutation Lavender Top (EDTA) tubes. Ship refrigerated. DO should be with prior *MTHFR gene NOT FREEZE. Overnight fasting is preferred. Duly consent from the mutation filled Coagulation Requisition Form is mandatory. treating Physician.

PAGE 375 OF 399 It is recommended 6 mL Whole blood in 2 Blue Top (Sodium that patient Citrate) tubes. Mix thoroughly by inversion. discontinues *Protein C Transport to Lab within 4 hrs. If this is not Heparin for 1 day Functional possible, make PPP within 1 hour of collection and Oral *Protein S as follows: Centrifuge sample at 3600 rpm for Anticoagulants for 7 Sample by 2nd Electromechanical & THROMBOTIC Functional 15 min & transfer supernatent to a clean plastic days prior to / 4th Sat 11 H246 Photo-optical Clot RISK SCREEN *Antithrombin tube. Centrifuge this supernatant again at 3600 sampling as these am; Report Next Detection Activity rpm for 15 mins & finally transfer the supernatant drugs may affect Friday Functional (PPP) to 1 labelled, clean plastic screw capped test results. *APCR vial. FREEZE IMMEDIATELY. Ship frozen.DO NOT Discontinuation THAW. Overnight fasting is preferred. Duly filled should be with prior Coagulation Requisition Form is mandatory. consent from the treating Physician.

*Free T3 *Free THYROID 3 mL (1.5 mL min.) serum from 1 SST. Ship Chemiluminescent Z045 T4 *TSH, Daily PROFILE, FREE refrigerated or frozen. Immunoassay Ultrasensitive

2 mL (1 mL min.) serum from 1 SST. Ship THYROGLOBULI refrigerated or frozen. At least 6 weeks should Chemiluminescent F012 Daily N; Tg elapse after thyroidectomy or needle biopsy, or Immunoassay I131 therapy before specimen collection.

PAGE 376 OF 399 Not the recommended test for Thyroid THYROID *T3 Total *T4 3 mL (1.5 mL min.) serum from 1 SST. Ship Chemiluminescent disorders. Use Test Z284 Daily PROFILE, TOTAL Total *TSH refrigerated or frozen. Immunoassay Code Z045 Thyroid Profile Free for better assessment of Thyroid Disorders. *Thyroid Profile, THYROID, Free * 5 mL (3 mL min.) serum from 2 SST’s. Ship Chemiluminescent Z120 COMPREHENSIV Daily Antithyroid refrigerated or frozen. Immunoassay E PANEL antibodies TISSUE Sample by Thu TRANSGLUTAMI 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S251 9 am; Report NASE (tTG) refrigerated or frozen Immunoassay Same day ANTIBODY, IgG TISSUE Sample by Thu TRANSGLUTAMI 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S228 9 am; Report NASE TTG-DGP refrigerated or frozen Immunoassay Same day SCREEN TISSUE Sample Daily by TRANSGLUTAMI 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S196 9 am; Report NASE (tTG) refrigerated or frozen Immunoassay Same day ANTIBODY, IgA

TLC (TOTAL 5 mL (2 mL min.) Fluid in a sterile screw P083 LEUCOCYTE capped container. Ship refrigerated. DO NOT Manual count Daily COUNT), FLUID FREEZE. TLC (TOTAL 3 mL (1 mL min.) whole blood in 1 Lavender Electrical H003 LEUCOCYTE Top (EDTA) tube. Ship refrigerated. DO NOT Daily Impedence, VCS COUNT) FREEZE.

PAGE 377 OF 399 3 mL (2 mL min.) serum from 1 Red Top (No Sample by 7th Additive) tube. Draw sample at Peak (2-4 hrs of the month; RW253 TOPIRAMATE LC-MS/MS after dose) or Trough (0.5-1 hr before dose) at Report after 2–3 steady state. Ship refrigerated or frozen. weeks *Toxoplasma *Rubella TORCH PANEL, 3 mL (1.5 mL min.) serum from 1 SST. Ship Z026 *Cytomegaloviru CLIA Daily IgM refrigerated or frozen. s *Herpes Simplex 1+2 *Toxoplasma Sample Daily by TORCH PANEL *Rubella 3 mL (1.5 mL min.) serum from 1 SST. Ship Enzyme B155 9 am. Report AVIDITY, IgG *Cytomegaloviru refrigerated or frozen. Immunoassay Same day. s

*Toxoplasma IgG & IgM *Rubella IgG & IgM TORCH PANEL, *Cytomegaloviru Sample by Tue / 3 mL (1.5 mL min.) serum from 1 SST. Ship Z776 IgG & IgM s IgG & IgM CLIA, EIA Fri 9 am; Report refrigerated or frozen. EXTENDED *Herpes Simplex Same day 1 IgG & IgM * Herpes Simplex 2 IgG & IgM

PAGE 378 OF 399 *Toxoplasma IgM *Rubella IgM Sample by Tue / TORCH PANEL, *Cytomegaloviru 3 mL (1.5 mL min.) serum from 1 SST. Ship Z778 CLIA, EIA Fri 9 am; Report IgM EXTENDED s IgM *Herpes refrigerated or frozen. Same day Simplex 1 IgM * Herpes Simplex 2 IgM *Toxoplasma* 4 mL (2 mL min.) Plasma from 1 Lavender top Rubella* Sample by TORCH DNA (EDTA) tube OR 3 mL (2 mL min.) CSF/ Amniotic fluid OS194 Cytomegalovirus PCR Wed/Sat 11 am; DETECTION in a sterile screw capped container. Ship * Herpes Report Fri/Tue refrigerated. DO NOT FREEZE. simplex* *Toxoplasma *Rubella TORCH PANEL, 5 mL (3 mL min.) serum from 2 SST’s. Ship Z028 *Cytomegaloviru CLIA Daily IgG & IgM refrigerated or frozen. s *Herpes Simplex 1+2

*Toxoplasma IgG *Rubella IgG *Cytomegaloviru Sample by Tue / TORCH PANEL, 3 mL (1.5 mL min.) serum from 1 SST. Ship Z777 s IgG *Herpes CLIA, EIA Fri 9 am; Report IgG EXTENDED refrigerated or frozen. Simplex 1 IgG * Same day Herpes Simplex 2 IgG

*Toxoplasma *Rubella TORCH PANEL, 3 mL (1.5 mL min.) serum from 1 SST. Ship Z027 *Cytomegaloviru CLIA Daily IgG refrigerated or frozen. s *Herpes Simplex 1+2

PAGE 379 OF 399 TOXICOLOGY Sample by 7th 5 mL (3 mL min.) serum from 2 Red Top (No Gas SCREEN, of the month; RW186 Additive) tubes . Do not use SST gel barrier Chromatography, BLOOD; DRUG Report after 2–3 tubes. Ship refrigerated or frozen. Immunoassay SCREEN weeks TOXOPLASMA ANTIBODIES 2 mL (1 mL min.) serum from 1 SST. Ship Z197 CLIA Daily PANEL, IgG & refrigerated or frozen. IgM TOXOPLASMA 2 mL (1 mL min.) serum from 1 SST. Ship S015 CLIA Daily ANTIBODY, IgM refrigerated or frozen.

4 mL(2 mL min.)Whole blood from 1 Lavender top TOXOPLASMA (EDTA) tube OR 3 mL (2 mL min.) Amniotic fluid Sample Daily by DNA OS195 /CSF/ Urine /Cord blood in a sterile screw capped PCR 11 am; Report 8 DETECTION , container OR Chorionic Villus biopsy in normal days PCR saline. Ship refrigerated. DO NOT FREEZE.

TOXOPLASMA 2 mL (1 mL min.) serum from 1 SST. Ship S039 CLIA Daily ANTIBODY, IgG refrigerated or frozen.

Sample Daily by TOXOPLASMA 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme B157 9 am; Report AVIDITY, IgG refrigerated or frozen. Immunoassay Same day. TPHA; Sample by Mon TREPONEMA 2 mL (1 mL min.) serum from 1 SST. Ship / Wed / Fri 9 S001 PALLIDUM Hemagglutination refrigerated or frozen. am; Report HEMAGGLUTIN Same day ATION

PAGE 380 OF 399 TPMT Sample by Mon (THIOPURINE 4 mL (2 mL min.) Whole blood in 1 Lavender / Thu 11 am; N071 METHYL Top (EDTA) tube. Ship refrigerated. DO NOT Real Time PCR Report Wed / TRANSFERASE) FREEZE Sat GENOTYPING t(12;21) (p13;q22); Sample by Mon 3 mL (2 mL min.) Whole blood / Bone marrow ETV6-RUNX1 / Thu 11 am; N063 in 1 Lavender Top (EDTA) tube. Ship refrigerated. Real Time PCR (TEL-AML1), Report Wed / DO NOT FREEZE. Clinical history is mandatory PCR Sat QUALITATIVE t(9;11) (p21- Sample by Mon 3 mL (2 mL min.) Whole blood / Bone marrow 22;q23); (MLL- / Thu 11 am; N066 in 1 Lavender Top (EDTA) tube. Ship refrigerated. Real Time PCR AF9), PCR Report Wed / DO NOT FREEZE. Clinical history is mandatory QUALITATIVE Sat t(11;19) Sample by Mon 3 mL (2 mL min.) Whole blood / Bone marrow (q23;p13.3); / Thu 11 am; N065 in 1 Lavender Top (EDTA) tube. Ship refrigerated. Real Time PCR (MLL-ENL), PCR Report Wed / DO NOT FREEZE. Clinical history is mandatory. QUALITATIVE Sat

t(1;19) Sample by Mon (q23;p13.3); 3 mL (2 mL min.) Whole blood / Bone marrow / Thu 11 am; N062 TCF3- BX1(E2A- in 1 Lavender Top (EDTA) tube. Ship refrigerated. Real Time PCR Report Wed / PBX1), PCR DO NOT FREEZE. Clinical history is mandatory. Sat QUALITATIVE

t(4;11) Sample by Mon 3 mL (2 mL min.) Whole blood / Bone marrow (q21;q23); / Thu 11 am; N064 in 1 Lavender Top (EDTA) tube. Ship refrigerated. Real Time PCR (MLL-AF4), PCR Report Wed / DO NOT FREEZE. Clinical history is mandatory. QUALITATIVE Sat

PAGE 381 OF 399 2 mL (1 mL min.) serum from 1 SST. Overnight H128 TRANSFERRIN Immunoturbidimetry Daily fasting is preferred. Ship refrigerated or frozen. Sample by 7th 2 mL (1 mL min.) serum from 1 SST. Ship of the month; RW177 T3, REVERSE LC/MS/MS refrigerated or frozen. Report after 2–3 weeks Therapeutic drug monitoring 3 mL (2 mL min.) serum from 1 Red Top (No TRICYCLIC for Sample by 7th Additive) tube. Do not use SST gel barrier tubes. ANTIDEPRESSA Amitriptyline, of the month; RW255 Ship refrigerated or frozen. Recommended LC-MS/MS NTS SCREEN, Nortriptyline, Report after 2–3 specimen: Trough levels > 12 hours after dose at SERUM Imipramine, weeks a steady state. Desipramine & Doxepin. 2 mL (1 mL min.) fluid in a sterile screw capped TRIGLYCERIDES, container. Ship refrigerated or frozen. Specify P055 GPO-POD Daily FLUID type of fluid on sample container and test request form. 2 mL (1 mL min.) serum from 1 SST. Ship TRIGLYCERIDES, Also see LIPID B026 refrigerated or frozen. Minimum 12 hours GPO-POD Daily SERUM PROFILE TESTS. overnight fasting is mandatory. 2 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th Additive) tube OR 2 mL (1 mL min.) Plasma from 1 of the month; RW188 TRIMIPRAMINE LC-MS/MS Green Top (Sodium Heparin) tube. Do not use SST Report after 2–3 gel barrier tubes. Ship refrigerated or frozen. weeks

TROPONIN - I, 2ml (1 ml min) serum in 1 SST. Ship refrigerated or Serum is an B110 HIGH SENSITIVE CMIA Daily frozen. acceptable sample. (hsTnI)

PAGE 382 OF 399 TROPONIN - T, 2 mL (1 mL min.) serum from 1 SST. Ship Electrochemilumines B085 Daily HIGH SENSITIVE refrigerated or frozen. cence

Patients who have undergone in-vivo Sample by 7th 2 mL (1 mL min.) serum from 1 SST. Ship isotope therapy of the month; RW189 TRYPSIN Immunoassay refrigerated or frozen. should refrain from Report after 2–3 testing for atleast weeks 6 weeks.

2 mL (1 mL min.) plasma from 1 Green Top (Sodium Sample by 7th of TRYPTOPHAN, Liquid Heparin) tube. Separate plasma and freeze within 2 the month; RW191 QUANTITATIVE, Chromatography, hours of collection. Ship frozen. DO NOT THAW. Reportafter 2–3 PLASMA Mass Spectrometry Overnight fasting ismandatory. weeks 2 mL (1 mL min.) serum from 1 Red Top (No Sample by 7th of Additive) tube. Separate serum within 2 hours of Fluorescence the month; RW190 TRYPTASE collection. Do not use SST gel barrier tubes Ship Immunoassay Reportafter 2–3 refrigerated or frozen. weeks Sample by 7th of 2 mL (1 mL min.) serum from 1 SST. Ship the month; RW192 TSH ANTIBODY Radioimmunoassay refrigerated or frozen. Reportafter 2–3 weeks 1 drop of heel prick blood each on 3 spots of Sample by Tue TSH, filter paper available from LPL. LPL. Clinical details / Thu / Sat 5 R159 NEWBORN Fluoroimmunoassay and drug history must accompany sample. Ship pm; Report 2 SCREEN refrigerated or frozen. days

Sample Daily by 4 TSH RECEPTOR 2 mL (1 mL min.) serum from 1 SST. Ship S229 ECLIA pm; Report ANTIBODY refrigerated or frozen. Same day

PAGE 383 OF 399 2 mL (1 mL min.) serum from 1 SST for each timed Refer to Y TSH specimen. Draw fasting baseline specimen; Codes:Y117 STIMULATION administer TRH as IV bolus 7 ug/kg body weight BasalY118 30 BY TRH (maximum 500 ug) over 30 sec. Draw additional Chemiluminescent min.Y119 60 Z274 Daily (THYROTROPIN specimens at 30 & 60 min. Ship refrigerated or Immunoassay min.Y120 RELEASING frozen. All containers must be clearly marked Customized HORMONE) with time drawn. Submit all specimens with 1 Injection TRH is not test request form. supplied by LPL

TSH (THYROID Sample to be given 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent R052 STIMULATING at the same time in Daily refrigerated or frozen. Immunoassay HORMONE) followup cases TSH (THYROID STIMULATING Sample to be given 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent R092 HORMONE), at the same time in Daily refrigerated or frozen. Immunoassay ULTRASENSITIV followup cases E TSI; THYROID Sample by 7th STIMULATING 2 mL (1 mL min.) serum from 1 SST. Ship of the month; RW194 Immunoassay IMMUNOGLOB refrigerated or frozen. Report after 2–3 ULIN weeks

PAGE 384 OF 399 Not the recommended test for Thyroid disorders as it is not the active 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent R050 T3, TOTAL form of the Daily refrigerated or frozen. Immunoassay hormone. Use Test Code R090 T3, Free for better assessment of T3 levels.

Not the recommended test for Thyroid disorders as it is not the active 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent R051 T4, TOTAL form of the Daily refrigerated or frozen. Immunoassay hormone. Use Test Code R091 T4, Free for better assessment of Thyroid disorders.

PAGE 385 OF 399 Not the recommended test for Thyroid disorders as it is not the active T3 & T4, 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent Z392 form of the Daily TOTAL refrigerated or frozen. Immunoassay hormone. Use Test Code Z194 FT3 & FT4 for better assessment of Thyroid disorders. TUMOUR Sample by Mon NECROSIS 2 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent B146 4 pm; Report FACTOR (TNF), refrigerated or frozen. Immunoassay Same day ALPHA 2 mL (1 mL min.) serum from 1 SST. Ship Immunochromatogr H062 TYPHI DOT Daily refrigerated or frozen. aphy Sample Daily by TYPHOID DNA 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS196 PCR 11 AM; REPORT DETECTION (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. 8 DAYS TYPHUS FEVER Sample by 7th (RICKETTSIA) 2 mL (1 mL min.) serum from 1 SST. Ship Immunofluorescence of the month; RW195 ANTIBODIES, refrigerated or frozen. Assay Report after 2–3 IgG & IgM weeks UGT1A1 GENE POLYMORPHIS Sample Daily by M 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS197 PCR 11 am; Report 14 (NUCLEOTIDE (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. days 'TA' REPEATS) DETECTION

PAGE 386 OF 399 10 mL (5 mL min.) aliquot of random urine in a sterile screw capped container. No preservative UREA CYCLE required. AND 2 mL (1 mL min.) plasma from 1 Sample Daily by G180 DISORDER Green Top (Sodium Heparin) tube. Transfer LC-MS/MS, GC/MS 5 pm; Report 5 PANEL plasma into a sterile screw capped vial. Ship days refrigerated or frozen. Clinical details and drug history must accompany sample.

2 mL (1 mL min.) fluid in a sterile screw capped P087 UREA, FLUID Urease UV Daily container. Ship refrigerated or frozen.

10 mL (5 mL min.) aliquot of 24 hour urine. Do not UREA, 24 use preservatives. Ship refrigerated or frozen. U028 Urease UV Daily HOUR URINE Refrigerate during and after collection. Record 24- hour volume on container and test request form. 10 mL (5 mL min.) aliquot of 24 hour urine. Do not UREA use preservatives. Ship refrigerated or frozen. U026 NITROGEN, 24 Refrigerate during and after collection. Record 24 Urease UV Daily HOUR URINE hour volume on test request form and urine container. UREA NITROGEN, 10 mL (5 mL min.) aliquot of random urine. Ship U102 Urease UV Daily RANDOM refrigerated or frozen. URINE UREA, 10 mL (5 mL min.) aliquot of random urine. Ship U076 RANDOM Urease UV Daily refrigerated or frozen. URINE 2 mL (1 mL min.) serum from 1 SST. Ship B021 UREA, SERUM Urease UV Daily refrigerated or frozen. URIC ACID, 2 mL (1 mL min.) serum from 1 SST. Ship B024 Uricase Daily SERUM refrigerated or frozen.

PAGE 387 OF 399 10 mL (5 mL min.) aliquot of 24 hour urine. Do not URIC ACID, 24 use preservatives. Ship refrigerated or frozen. U010 Uricase Daily HOUR URINE Record 24 hour volume on test request form and urine container. URIC ACID, 10 mL (5 mL min.) aliquot of random urine. Ship U078 RANDOM Uricase Daily refrigerated or frozen. URINE URINE 10 mL (5 mL min.) aliquot of first morning urine in Automated Strip EXAMINATION, U001 a sterile screw capped container. Ship test, Chemical, Daily ROUTINE; refrigerated. Light microscopy URINE, R/E 10 mL (5 mL min.) aliquot of first morning urine in URINE FOR U065 a sterile screw capped container. Ship Light microscopy Daily EOSINOPHILS refrigerated. 10 mL (5 mL min.) aliquot of first morning urine in Automated strip For Post Prandial URINE, U082 a sterile screw capped container. Ship test / Benedicts urine, use test Daily GLUCOSE refrigerated. Test code U002 URINE, 10 mL (5 mL min.) aliquot of random urine in a Sample Daily by Phase Contrast U066 DYSMORPHIC sterile screw capped container. Ship refrigerated. 2 pm; Report Microscopy RBC DO NOT FREEZE. Same day URINE, 10 mL (5 mL min.) aliquot of first morning urine in U080 REDUCING a sterile screw capped container. Ship Dipstick, Benedict’s Daily SUBSTANCES refrigerated. Also See Sample by Tue / U1RNP 2 mL (1 mL min.) serum from 1 SST. Overnight Enzyme S068 EXTRACTABLE Fri 9 am; ANTIBODIES fasting is preferred. Ship refrigerated or frozen. Immunoassay NUCLEAR ANTIGENS. Report Same day 10 mL (5 mL min.) aliquot of 24 hour urine without UROBILINOGEN, preservative, in a dark coloured container. Ship U061 QUALITATIVE, refrigerated or frozen. Keep specimen Dipstick Daily 24 HOUR URINE refrigerated during collection and protect from light.

PAGE 388 OF 399 2 mL (1 mL min.) serum from 1 Red Top (No VALPROIC Additive) tube. Do not use SST gel barrier tubes. Chemiluminescent D031 ACID; Ship refrigerated or frozen. Recommended Daily Immunoassay VALPROATE specimen: just before the next dose is due at steady state. Sample Daily by 4 2 ml (1 ml. min) serum from 1 SST. Ship refrigerated D053 VANCOMYCIN CMIA pm; Report or frozen. Same Day VANCOMYCIN RESISTANT Sample Daily by 1 M220 ENTEROCOCCI Rectal / Wound SWAB / Pure ISOLATE Chromogenic pm; Report Next (VRE) day SCREENING VARICELLA ZOSTER VIRUS *Chicken Pox * Sample by Mon (VZV) 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme Z199 Herpes Zoster * / Thu 9 am; ANTIBODIES refrigerated or frozen. immunoassay Shingles Report Same day PANEL, IgG & IgM VARICELLA *Chicken Pox * Sample by Mon ZOSTER VIRUS 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S077 Herpes Zoster / Thu 9 am; (VZV) refrigerated or frozen. Immunoassay *Shingles Report Same day ANTIBODY, IgG

VARICELLA *Chicken Pox Sample by Mon ZOSTER VIRUS 2 mL (1 mL min.) serum from 1 SST. Ship Enzyme S074 *Herpes Zoster / Thu 9 am; (VZV) refrigerated or frozen. Immunoassay *Shingles Report Same day ANTIBODY, IgM

PAGE 389 OF 399 VARICELLA ZOSTER VIRUS 3 ml (2 mL min.) whole blood from 1 Lavender Top Sample by N128 (VZV), (EDTA) tube OR 3 ml (2 mL min.) CSF. Ship Real Time PCR Mon/Thu 11 am; QUALITATIVE refrigerated. DO NOT FREEZE. Report Wed/Sat PCR *ANA *ANCA- 3 mL (2 mL min.) serum from 1 SST. Ship Sample by Tue / VASCULITIS MPO *ANCA- Z613 refrigerated or frozen. Overnight fasting is EIA Thu / Sat 9 am, PANEL PR3 *Anti - ds- preferred Report Same day DNA VASOACTIVE 3 mL (1.5 mL min.) plasma from1 Lavender Top Sample by 7th INTESTINAL (EDTA) tube. Separate plasma within 2 hours of Extraction, of the month; RW196 POLYPEPTIDE; collection in a plastic transport vial and freeze Immunoassay Report after 2–3 VIP immediately. Ship frozen. DO NOT THAW. weeks

2 mL (1 mL min.) Cerebrospinal fluid in a sterile S011 VDRL, CSF Slide Flocculation Also see STD Panel Daily screw capped container. Ship refrigerated or frozen. VDRL (RPR), 2 mL (1 mL min.) serum from 1 SST. Ship S008 Slide Flocculation Also see STD Panel Daily SERUM refrigerated or frozen.

*Factor V Leiden Mutation Sample by Mon VENOUS 5 mL (3 mL min.) whole blood in 2 Lavender *Prothrombin / Thu 11 am; N018 THROMBOSIS Top (EDTA) tubes. Ship refrigerated. DO NOT Real Time PCR Gene Mutation Report Wed / RISK FREEZE. *MTHFR Gene Sat Mutation

PAGE 390 OF 399 VGKCs are a family of voltage-gated potassium channels & are membrane proteins - Leucine- VGKC rich glioma (VOLTAGE inactivated protein Sample by GATED 2 mL (1 mL min) serum from 1 SST. Ship refrigerated S250 Immunofluorescence 1(LGI1) & Contactin- Monday 11 am. POTASSIUM of frozen. Overnight fasting is preferred. associated protein 2 Report 5 days CHANNEL) , (CASPR2), ANTIBODY responsible for controlling the cell membrane potential.These antigens play a direÇ

Sample by 7th VISCOSITY, 3 mL (1.5 mL min.) serum from 1 SST. Ship of the month; RW197 Kinetic Viscometer SERUM refrigerated or frozen. Report after 2–3 weeks VITAMIN B12 BINDING 2 mL (1 mL min.) serum from 1 SST. Separate Sample by 7th CAPACITY, serum within 1 hour of collection and transfer of the month; RW200 UNSATURATED; Radiobinding Assay to a plastic transport vial. Ship refrigerated or Report after 2–3 frozen. weeks TRANSCOBALA MIN

PAGE 391 OF 399 VITAMIN B12, Sample by Mon / ACTIVE; 2 mL (1 mL min.) serum from 1 SST. Ship R174 CMIA Thu 4 pm ; HOLOTRANSCO refrigerated or frozen. Report Same day BALAMIN This assay This assay VITAMIN D 25 measures both 3 mL (1.5 mL min.) serum from 1 SST. Ship R119 Chemiluminescence measures both D2 Daily - HYDROXY D2 and D3 refrigerated or frozen. and D3 components. components. *Calcium *Phosphorus 3 mL (2 mL min.) serum from 1 SST. Avoid use of VITAMIN D *Alkaline tourniquet and fist clenching during sampling. CLIA, Z617 COMPREHENSIV Daily Phosphatase Overnight fasting is preferred. Ship refrigerated or Spectrophotometry E *Vitamin D 25 frozen. Hydroxy 3 mL (2 mL min.) serum from 1 SST. Avoide use of *Calcium VITAMIN D tourniquet and fist clenching during sampling. CLIA, Z616 *Vitamin D 25 Daily SCREEN Overnight fasting is preferred. Ship refrigerated or Spectrophotometry Hydroxy frozen. 3 mL (2 mL min.) Whole blood / Plasma from 1 Lavender Top (EDTA) tube OR 2 mL (1 mL min.) serum from one Red Top (No Additive) tube Sample by 7th VITAMIN B1 Transfer whole blood to plastic transport vial and of the month; RW199 LC-MS/MS (THIAMINE) freeze immediately. Separate plasma / serum within Report after 2–3 4 hours of collection and freeze. Ship frozen. DO weeks NOT THAW. Wrap in aluminium foil to protect from light. Overnight fasting is preferred. 4 mL (2 mL min.) plasma from 2 Green Top (Sodium Heparin) tubes. Separate plasma from cells Sample by 7th High Performance within 1 hour of collection and FREEZE. Ship of the month; RW204 VITAMIN K Liquid frozen. DO NOT THAW. Wrap in aluminium foil Report after 2–3 Chromatography to protect from light. Overnight fasting is weeks preferred.

PAGE 392 OF 399 2 mL (1 mL min.) plasma from 1 Lavender Top High Performance (EDTA) tube. Separate plasma from cells within 1 Sample by 7th Liquid VITAMIN B2; hour of collection and transfer to a plastic of the month; RW201 Chromatography, RIBOFLAVIN transport vial. Freeze immediately. Ship frozen. DO Report after 2–3 Fluorometric NOT THAW. Wrap in aluminium foil to protect weeks detection from light. 3 mL (1.5 mL min.) serum from 1 Red Top (No Additive) tube. Separate serum from cells within High Performance VITAMIN E; Sample by Fri 9 R117 1 hour of collection. Ship refrigerated or frozen. Liquid TOCOPHEROL am; Report Tue Protect from light by wrapping in aluminium foil. Chromatography Overnight fasting is mandatory. 2 mL (1 mL min.) plasma from 1 Lavender Top (EDTA) tube. Separate plasma and freeze Sample by 7th immediately. Ship frozen. DO NOT THAW. Wrap VITAMIN B6; of the month; RW202 tube in aluminium foil to protect from light. LC-MS/MS PYRIDOXINE Report after 2–3 Avoid vitamin supplements & alcohol 24 hours weeks prior to sample collection. Overnight fasting is mandatory.

2 mL (1 mL min.) serum from 1 SST. Separate serum within 3 hours of collection in a plastic transport Sample by 7th VITAMIN C; vial and freeze. Ship frozen. DO NOT THAW. Wrap Capillary of the month; RW203 ASCORBIC ACID in aluminium foil to protect from light. Avoid Electrophoresis Report after 2–3 vitamin supplements & fruits 24 hours prior to weeks sample collection. Overnight fasting is preferred.

This assay Sample by Tue This assay VITAMIN D 1, measures both 3 mL (1.5 mL min.) serum from 1 SST. Ship / Thu / Sat 9 am; R118 CLIA measures both D2 25-DIHYDROXY D2 and D3 refrigerated or frozen. Report Same and D3 components. components. day

PAGE 393 OF 399 3 mL (1.5 mL min.) serum from 1 Red Top (No Additive) tube. Separate serum within 1 hour of High Performance Sample by Fri 9 VITAMIN A R096 collection. Ship refrigerated or frozen. Wrap in Liquid Also see CAROTENE. am; Report on (RETINOL) aluminium foil to protect from light. Overnight Chromatography Tue fasting is mandatory. VITAMIN B12; 3 mL (1 mL min.) serum from 1 SST. Ship Chemiluminescent Also see FOLATE, R047 CYANOCOBALA refrigerated or frozen. Wrap in aluminium foil to Daily Immunoassay SERUM. MIN protect from light. Overnight fasting is mandatory. 25-Hydroxy VITAMIN D, Vitamin D2, D3 Sample Daily by 3 mL (1.5 mL min.) serum from 1 SST. Ship G183 ULTRASENSITIV & 3 epi 25 LC-MS/MS 5 pm; Report refrigerated or frozen. E Hydroxy Vitamin Same day D3 50 mL (10 mL min.) aliquot of 24 hour urine. Collect urine with appropriate volume of 50% HCl to maintain pH between 1-2. Do not use VMA concentrated HCl. Record 24 hour volume on test High Performance Also see Sample by Wed (VANILMANDELI request form and urine container. Ship U006 Liquid PHEOCHROMOCYTO / Sat 9 am; C ACID), 24 refrigerated or frozen. Patient should strictly avoid Chromatography MA PROFILE. Report 2 days HOUR URINE Theophylline, chocolate, vanilla, banana, alcoholic beverages, tea/coffee, tobacco and strenuous exercise at least 72 hrs before and during specimen collection.

50 mL (10 mL min.) aliquot of random urine. Add 50% HCl drop by drop to bring the pH between VMA 1–2. Do not use concentrated HCl. Ship (VANILMANDELI High Performance Also see Sample by Wed refrigerated or frozen. Patient should strictly avoid U030 C ACID), Liquid PHEOCHROMOCYTO / Sat 9 am; Theophylline, chocolate, vanilla, banana, alcoholic RANDOM Chromatography MA PROFILE. Report 2 days beverages, tea/coffee, tobacco and strenuous URINE exercise at least 72 hrs before and during specimen collection.

PAGE 394 OF 399 It is recommended 3 mL Whole blood in 1 Blue Top (Sodium that patient Citrate) tube. Mix thoroughly by inversion. discontinues Transport to Lab within 4 hrs. If this is not Heparin for 1 day possible, make PPP within 1 hour of collection as and Oral von follows: Centrifuge sample at 3600 rpm for 15 Anticoagulants for 7 WILLEBRAND Sample by 1st min & transfer supernatent to a clean plastic Enzyme days prior to H130 FACTOR & 3rd Mon; tube. Centrifuge this supernatant again at 3600 Immunoassay sampling as these ANTIGEN; vWF Report Next day rpm for 15 mins & finally transfer the supernatant drugs may affect Ag (PPP) to 1 labelled, clean plastic screw capped test results. vial. FREEZE IMMEDIATELY. Ship frozen.DO NOT Discontinuation THAW. Overnight fasting is preferred. Duly filled should be with prior Coagulation Requisition Form is mandatory. consent from the treating Physician.

WARFARIN Sample by Sat 11 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS199 SENSITIVITY PCR Sequencing am; Report 8 (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. DETECTION days

Also see BRUCELLA *Proteus ANTIBODY, FEBRILE Sample Daily by WEIL FELIX 2 mL (1 mL min.) serum from 1 SST. Ship S002 Antigen OX19, Latex Agglutination. AGGLUTININS 3.30 pm; Report TEST refrigerated or frozen. OX2 & OXK PANEL & WIDAL Next day TEST.

Also see BRUCELLA WIDAL SLIDE ANTIBODY, FEBRILE 2 mL (1 mL min.) serum from 1 SST. Ship S026 AGGLUTINATIO Slide Agglutination AGGLUTININS Daily refrigerated or frozen. N TEST PANEL & WEIL FELIX TEST.

PAGE 395 OF 399 Also see BRUCELLA WIDAL TUBE ANTIBODY, FEBRILE Sample Daily by 2 mL (1 mL min.) serum from 1 SST. Ship S199 AGGLUTINATIO Tube Agglutination AGGLUTININS 3.30 pm; Report refrigerated or frozen. N TEST PANEL & WEIL FELIX Next day TEST.

WILSON DISEASE ATP7 Sample Daily by 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS200 B GENE PCR 11 am ; Report (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. MUTATION 30 days DETECTION

Use only specified 3 mL (1 mL min.) Whole blood in 1 Lavender Top containers available (EDTA) tube AND 2 mL (1.5 mL min.) Serum from 1 *CBC *LFT Spectrophotometry, from LPL. If barium SST AND 3 mL (1.0 mL min.) Serum from 1 Metal Sample Mon *Ceruloplasmin* Nephelometry, containing contrast WILSON free White top (Z- No additive) tube available from through Sat 6 Z834 Copper. Serum Electrical media has been DISEASE PANEL LPL AND 10 mL (5 mL min.) aliquot of random urine pm ; Report *Copper. Impedence, VCS, administered, the collected in an acid washed (metal free) container Next day Random Urine ICPMS specimen should not available from LPL. Ship refrigerated. Use be collected for 96 powderless gloves during specimen collection. hours.

WT-1 4 mL (2 mL min.) Whole Blood/ Bone marrow in 1 Sample by Wed 9 OS201 MUTATION Lavender top (EDTA) Tube. Ship refrigerated. DO PCR Sequencing a.m report after DETECTION NOT FREEZE. Clinical history is mandatory 8 days

PAGE 396 OF 399 Y- CHROMOSOME Sample by Wed 9 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS202 MICRODELETIO Multiplex PCR a.m report after (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. N DETECTION , 9 days PCR

YEAST Sample Daily by (CANDIDA Rapid Automated M135 Positive culture growth. Ship refrigerated. 3.30 pm; Report SPECIES) Identification 3 days IDENTIFICATION YEAST (CANDIDA Sample Daily by Rapid Automated M136 SPECIES) Positive culture growth. Ship refrigerated. 3.30 pm; Report Susceptibility testing SUSCEPTIBILITY 3 days TESTING YERSINIA Sample Daily by 1 5 g fresh STOOL in a sterile screw capped container. M216 ENTEROCOLITIC ICT pmReport Same Ship refrigerated only. DO NOT FREEZE. A ANTIGEN day

Use only specified 10 mL (5 mL min.) aliquot of 24 hour urine containers available collected in an acid washed (metal free) from LPL. If container kit available from LPL. Mix thoroughly. gadolinium or iodine Transfer 10 mL aliquot to vial provided in the Sample Daily by ZINC, 24 HOUR containing contrast C033 kit. Measure 24 hour total volume and record ICPMS 6 pm; Report URINE media has been on vial and test request form. Ship refrigerated Next day administered, the or frozen. Patients should refrain from sea/ fresh specimen should water fish 3 days prior to specimen collection. Use not be collected for powderless gloves during specimen collection. 96 hours.

PAGE 397 OF 399 Use only specified containers available 10 mL (5 mL min.) aliquot of random urine from LPL. If collected in an acid washed (metal free) gadolinium or iodine ZINC, Sample Daily by container available from LPL. Ship refrigerated or containing contrast C035 RANDOM ICPMS 6 pm; Report frozen. Patients should refrain from sea/ fresh media has been URINE Next day water fish 3 days prior to specimen collection. Use administered, the powderless gloves during specimen collection. specimen should not be collected for 96 hours.

Use only specified containers available 3 mL (1 mL min.) serum from 1 metal free White from LPL. If Top ( Z-No Additive) tube OR 3 mL (1 mL min.) gadolinium or iodine plasma from 1 metal free Royal Blue Top (K2EDTA) Sample Daily by ZINC, SERUM / containing contrast C036 tube available from LPL. Ship refrigerated or frozen. ICPMS 6 pm; Report PLASMA media has been Patient should avoid sea / fresh water fish 3 days Next day administered, the prior to specimen collection. Use powderless gloves specimen should during specimen collection. not be collected for 96 hours.

PAGE 398 OF 399 Miscellaneous specimens ( Sputum, Urine, Pus, Body Fluids like pleural, peritoneal, pericardial, gastric, bronchial, semen etc.) in a sterile, screw capped container OR Swabs in sterile normal saline OR Bone marrow in 1 Green Top (Sodium Heparin) Z-N STAIN; Conventional For tissue specimens Sample Daily by tube, OR smeared onto 4 clean glass slides fixed M191 ZIEHL NEELSEN microscopic use test code J050 3.30 pm; Report immediately in 95% ethanol for 30 minutes and air STAIN examination AFB Stain Same day dried. For gastric lavage , patient should be fasting for previous 12 hours. Transport the gastric lavage specimens within 4 hrs of collection. Specify type of specimen on container and test request form. Ship refrigerated.

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