Test Code Test Name Components Specimen Method Comments Report *CD3 *CD19 2 mL (1 mL min.) serum from 1 SST AND 6 mL (4 mL *CD16 + min.) whole blood in 2 Lavender Top (EDTA) tubes ABORTION CD56*TNF-alpha AND 3 mL (2 mL min.) whole blood in 1 Green Top Flow Cytometry, Sample by Mon 4 Z785 PANEL *HLA DR, DQA1 (Sodium Heparin) tube. Ship immediately at CLIA, PCR pm; Report Wed & DQB1 (Class II) 18–22°C. DO NOT REFRIGERATE OR FREEZE. Specify Typing time, date and clinical details on test request form. ABSOLUTE 3 mL (1 mL min.) whole blood in 1 Lavender Top Electrical H022 EOSINOPHIL Daily (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Impedance, VCS COUNT; AEC ABSOLUTE 3 mL (1 mL min.) whole blood in 1 Lavender Top Electrical H052 LYMPHOCYTE Daily (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Impedance, VCS COUNT ABSOLUTE 3 mL (1 mL min.) whole blood in 1 Lavender Top Electrical H051 NEUTROPHIL Daily (EDTA) tube. Ship refrigerated. DO NOT FREEZE. Impedance, VCS COUNT ACETYLCHOLINE Sample by 7th of RECEPTOR 2 mL (0.5 mL min.) serum from 1 Red Top (No the month; RW002 (ACHR) Immunoassay Additive) tube. Ship refrigerated or frozen. Report after 2–3 BLOCKING weeks ANTIBODY This assay should not be requested for patients who have ACETYLCHOLINE recently received RECEPTOR 2 mL (1 mL min.) serum from 1 SST. Ship radio-isotopes Sample by Wed / S220 (ACHR) refrigerated or frozen. Avoid general anaesthetic or Radioimunoassay therapeutically or Sat 9 am; Report BINDING muscle relaxant drugs 24 hours prior to sampling. diagnostically due to Same day ANTIBODY potential assay interference. Also see MYASTHENIA GRAVIS PANEL. PAGE 1 OF 399 ACETYLCHOLINE 2 mL (0.5 mL min.) serum from 1 Red Top (No Sample by 7th of RECEPTOR Additive) tube. Do not use SST gel barrier tubes. the month; RW003 (ACHR) Ship refrigerated or frozen. Avoid general Radiobinding Assay Report after 2–3 MODULATING anaesthetic or muscle relaxant drugs 24 hours prior weeks ANTIBODY to sampling. 3 ml (1 mL min.) amniotic fluid in a sterile screw ACETYLCHOLINE Sample by 7th of capped vial. Ship refrigerated or frozen. Indicate Sample should be STERASE, the month; RW004 gestational age, collection date, clinical indications Gel Electrophoresis drawn between 14- AMNIOTIC Report after 2–3 and the AFP and MoM results if available on the 21 weeks gestation FLUID weeks Test Request Form. *Acid 2 mL (0.5 mL min) serum from 1 SST. Keep whole ACID Phosphatase specimen refrigerated until serum can be separated. B054 PHOSPHATASE, Total*Acid Spectrophotometry Daily Separate serum, add 2 drops (60 uL) 5% Acetic acid PROSTATIC Phosphatase and mix. Ship refrigerated or frozen. Prostatic 2 mL (0.5 mL min.) serum from 1 SST. Keep whole ACID specimen refrigerated until serum can be separated. B053 PHOSPHATASE, Spectrophotometry Daily Separate serum, add 2 drops (60 uL) 5% Acetic acid TOTAL and mix. Ship refrigerated or frozen. 2 mL (0.5 mL min.) plasma from 1 Lavender Top (EDTA) tube. Draw blood between 8–10 a.m. Mix Sample Daily by 4 Chemiluminescent R040 ACTH well, separate and transfer plasma IMMEDIATELY pm; Report Same Immunoassay into a sterile screw capped vial and FREEZE. Ship day frozen. DO NOT THAW. PAGE 2 OF 399 It is recommended 3 mL Whole blood in 1 Blue Top (Sodium Citrate) that patient tube. Mix thoroughly by inversion. Transport to Lab discontinues within 4 hours. If this is not possible, make PPP Heparin for 1 day within 1 hour of collection as follows: Centrifuge and Oral ACTIVATED sample at 3600 rpm for 15 min & transfer Anticoagulants for 7 Sample by PROTEIN C - supernatent to a clean plastic tube. Centrifuge this Electromechanical days prior to 2nd/4th Sat by 9 E016 RESISTANCE; supernatant again at 3600 rpm for 15 mins & finally Clot Detection sampling as these am; Report Next APCR trasfer the supernatant (PPP) to 1 labelled, clean drugs may affect Mon plastic screw capped vial. FREEZE IMMEDIATELY. test results. Ship frozen. DO NOT THAW. Overnight fasting is Discontinuation preferred. Duly filled Coagulation Requisition Form should be with prior is mandatory. consent from the treating Physician ACTN3 (SPORTS Sample by Thu 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS100 GENE), PCR Sequencing 11 am; Report (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. GENOTYPING 10 days PAGE 3 OF 399 *t (8;21) or LSI ETO/AML1 * inv (16) (p13q22) or t(16;16) (p13;q22) or ACUTE CBFB * t (15;17) MYELOID 8 mL (6 mL min.) whole blood from 2 Green Top or LSI PML/RARA Sample Daily by 6 LEUKEMIA (Sodium Heparin ) tubes AND 4 mL (2 mL min.) FISH & Conventional Z774 * pm; Report 7 (AML), Bone Marrow from 1 Green Top (Sodium Heparin) Karyotyping t(variable;11q23) Days CYTOGENETICS tube. Ship at 18-22°C. DO NOT FREEZE. ; MLL gene PANEL breakapart *Chromosome analysis, Hematological malignancy *Cystatin C *Neutrophil Gelatinase - Associated CMIA, 5 mL ( 3 mL min.) serum from 1 SST AND 10 mL (5 Sample by Mon / ACUTE KIDNEY Lipocalin (NGAL) Nephelometry, Z805 mL min.) aliquot of random urine in a sterile screw Wed / Fri 4 pm; INJURY PANEL *Creatinine, Jaffe's reaction, capped container. Ship refrigerated or frozen. Report Same Day serum Indirect ISE *Fractional excretion of Sodium PAGE 4 OF 399 *BCR/ABL or Philadelphia translocation *t(variable;11q2 ACUTE 3) ; MLL gene LYMPHOBLASTI 8 mL (6 mL min.) whole blood from 2 Green Top breakapart Sample Daily by 6 C LEUKEMIA (Sodium Heparin ) tubes AND 4 mL (2 mL min.) FISH & Conventional Z775 * t (12;21) pm; Report 7 (ALL), Bone Marrow from 1 Green Top (Sodium Heparin) Karyotyping (p13;q22); Days CYTOGENETICS tube. Ship at 18-22°C. DO NOT FREEZE. TEL/AML1 PANEL *Chromosome analysis, Hematological malignancy 2 mL (1 mL min.) serum from 1 SST OR 2 mL (1 mL ADENOSINE min.) plasma from 1 Lavender Top (EDTA) tube OR 2 R156 DEAMINASE ; mL (1 mL min.) Body Fluid / CSF in a sterile screw Spectrophotometry Daily ADA capped container. Ship refrigerated or frozen. Hemolysed samples are not acceptable. ADENOVIRUS Sample Daily by 1 ANTIGEN FOR 5 g fresh STOOL in a sterile screw capped container. M211 ICT pmReport Same GASTROENTERI Ship refrigerated only. DO NOT FREEZE. day TIS. STOOL ADENOVIRUS, 3 ml (2 mL min.) whole blood from 1 Lavender Top Sample by N126 QUALITATIVE, (EDTA) tube OR 3 ml (2 mL min.) CSF. Ship Real Time PCR Mon/Thu 11 am; PCR refrigerated. DO NOT FREEZE. Report Wed/Sat ADENOVIRUS Sample Daily by 1 ANTIGEN FOR 5 g fresh STOOL in a sterile screw capped container. M213 ICT pmReport Same RESPIRATORY Ship refrigerated only. DO NOT FREEZE. day INFECTIONS PAGE 5 OF 399 ADRB2 GENOTYPING Sample by Sat 11 FOR BETA-2 4 mL (2 mL min.) Whole Blood in 1 Lavender top OS102 PCR Sequencing am; Report 10 AGONIST (EDTA) Tube. Ship refrigerated. DO NOT FREEZE. days RESPONSIVENES S This test can be used in conjunction with other AFB Cultures Submit pure growth of AFB on appropriate media in on specific request screw capped container in sealed plastic bag. Ship as additional test. AFB refrigerated OR when used with other AFB culture For Nucleic Acid Sample Daily by IDENTIFICATION Automated M094 tests, the pure growth obtained in the Laboratory Probes, use test 3.30 pm; Report , RAPID; PNB Fluorescent from specimens already submitted will be code N001; for AFB 15 days TEST processed for Mycobacterium TB Complex / MOTT Antibiotic indentification. Sensitivity, use appropriate test code. Request as additional tests. Miscellaneous specimens (Urine, Pus, Body Fluids like pleural, peritoneal, pericardial, gastric, bronchial, semen etc.) in a sterile, screw capped container OR Swabs in sterile normal saline OR Bone marrow in 1 Green Top (Sodium Heparin) tube Fluorescent and AFB STAIN, Auramine and For tissue specimens Sample Daily by OR smeared onto 4 clean glass slides fixed conventional P030 MISCELLANEOU Ziehl Neelsen use test code J050 3.30 pm; Report immediately in 95% ethanol for 30 minutes and air microscopic S staining AFB Stain Same day dried. For gastric lavage , patient should be fasting examination for previous 12 hours. Transport the gastric lavage specimens within 4 hrs of collection. Specify type of specimen on container and test request form. Ship refrigerated. PAGE 6 OF 399 AFP (ALPHA Also see MATERNAL FETOPROTEIN), 3 mL (1 min.) Amniotic fluid. Avoid contamination Chemiluminescent R084 SERUM SCREENING Daily AMNIOTIC with maternal blood. Ship refrigerated or frozen. Immunoassay PANELS. FLUID AFP (ALPHA Recommended for FETOPROTEIN), 2 mL (0.5 mL min.) serum from 1 SST. Ship Chemiluminescent F001 cancer follow up Daily TUMOR refrigerated or frozen. Give brief clinical history. Immunoassay only. MARKER The test is recommended only AFP (ALPHA between 14–22 2 mL (0.5 mL min.) serum from 1 SST. Ship Chemiluminescent R030 FETOPROTEIN), weeks of pregnancy. Daily refrigerated or frozen. Immunoassay MATERNAL Also see MATERNAL SERUM SCREENING PANELS. Submit Throat swabs in Aerobic transport medium Gram staining and P094 ALBERT STAIN available from LPL OR 2 air dried smears on sterile Metachromatic Daily glass slides. Ship refrigerated. DO NOT FREEZE. staining Sample Mon 2 mL (1 mL min.) Cerebrospinal fluid (CSF) in a through Sat by 4 B094 ALBUMIN, CSF sterile screw capped container. Ship refrigerated or Nephelometry pm; Report Same frozen. day ALBUMIN, 2 mL (1 mL min.) Body fluid in a sterile screw P086 BCG Daily FLUID capped container. Ship refrigerated or frozen. Sample Mon 2 mL (1 mL min.) Cerebrospinal fluid (CSF) in a ALBUMIN & *CSF IgG/ Also see MULTIPLE through Sat by 4 Z088 sterile screw capped container.