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Test Directory 800.541.7891 509.755.8600 Test Directory Fax 509.921.7107 Billing Code Test Code [sunquest] (1,3)-BETA-D-GLUCAN (FUNGITELL) 13BGA 13BGA Synonyms Fungitell; Glucan Container Type Red top tube (plain) Store and Transport Refrigerated Specimen Type Serum Preferred Volume 2 mL Minimum Volume 0.5 mL Specimen Processing Separate serum from cells within 2 hours of collection and transfer to a standard PAML aliquot tube Room Temp Unacceptable Refrigerated 2 weeks Frozen (-20°C) 2 weeks Unacceptable Condition Hemolyzed, lipemic and icteric samples Reference Laboratory ARUP Reference Lab Test Code 2002434 CPT Codes 87449 Test Schedule Mon-Fri Turnaround Time 2-4 days Method Semi-Quantitative Colorimetry Test Includes (1,3-beta-D-glucan, pg/mL; (1,3-beta D-glucan Interpretation. Notes Reference ranges for pediatric patients (less than 18 years old) have not been established. Assay ranges were validated in adult subjects. Supply Item Number 1372 Billing Code Test Code [sunquest] 1, 5 ANHYDROGLUCITOL (GLYCOMARK) GLYMAR GLYMAR Synonyms Anhydroglucitol, 1,5 AD; GlycoMark® Container Type Serum separator tube (gold, brick, SST, or corvac) Store and Transport Refrigerated Specimen Type Serum Preferred Volume 1 mL Minimum Volume 0.2 mL Specimen Processing Allow serum specimen to clot completely at room temperature. Separate serum or plasma from cells and transfer to a standard PAML aliquot tube Room Temp 1 week Refrigerated 1 week Frozen (-20°C) 1 month Alternate Specimens Lavender (EDTA) Reference Laboratory ARUP Reference Lab Test Code 0081335 CPT Codes 84378 Test Schedule Mon-Fri Turnaround Time 2-4 days Method Quantitative Enzymatic Test Includes GlycoMark, ug/mL. Supply Item Number 1467 Billing Code Test Code [sunquest] 11-DEOXYCORTISOL, LC/MS/MS 11DXC 11DXC Container Type Red top tube (plain) Store and Transport Transport frozen Specimen Type Frozen serum Preferred Volume 1 mL Minimum Volume 0.25 mL Patient Prep An early morning specimen is preferred Specimen Processing Separate serum from cells and put in a separate plastic tube. Room Temp 4 days Refrigerated 4 days Frozen (-20°C) 28 days Unacceptable Condition Sample collected in an SST tube. Do not submit glass tubes. Alternate Specimens Plasma, EDTA (lavender-top), EDTA (royal blue-top), Sodium heparin (green-top), Lithium heparin (green-top) Reference Laboratory Quest Diagnostics Nichols Institute (SJC) Reference Lab Test Code 30543X CPT Codes 82634 Test Schedule Sat Turnaround Time 4-12 days Method Liquid Chromatography Tandem Mass Spectrometry 2.1 www.paml.com 4/16/2013 page 1 1 800.541.7891 509.755.8600 Fax 509.921.7107 Test Directory 1 Billing Code Test Code [sunquest] 14-3-3 PROTEIN, CSF (PRION DISEASE) PRION PRION Synonyms Prion Disease; Protein 14-3-3, CJD; Creutzfeldt-Jacob Disease (CJD); Creutz-Jacob Disease; Transmissable Spongiform Encephalopathies (TSE) Container Type Sterile screw cap vial Store and Transport Frozen on dry ice. Ship Category B Specimen Type Frozen CSF Preferred Volume 5 mL Minimum Volume 1 mL Collection Procedure Collect CSF by lumbar puncture. Discard first 2 mL that flows from tap. Collect next 5 mL CSF, avoiding bloody tap. Specimen Processing The CSF should be frozen as quickly as possible (preferably within 20 minutes) and stored at -80C freezer (or, lacking that, in a -20C freezer) until shipment on dry ice. Required Patient Info Please complete and send the National Prion Requisition form, available on the PAML website at www.paml.com under the FORMS and BROCHURES link with the sample. Room Temp 20 minutes Refrigerated Unacceptable Frozen (-20°C) 1 year Frozen (-70°C) 1 year Reference Laboratory National Prion Disease Pathology Surveillance Center at Case Western Reserve University CPT Codes 86317, 84182 Test Schedule Mon-Fri Turnaround Time 16-21 days Method Immunoassay Test Includes 14-3-3 Protein, CSF Notes Patient is also asked to submit a frozen urine sample for validation of a recently published diagnostic test on Creutzfeldt-Jakob disease; no report will be issued. Please inform patient and/or family that urine is used for research on a diagnostic test and obtain oral consent. A recent re-evaluation of the recommendation to ship CSF specimens frozen with dry-ice showed shipment by Federal Express at ambient temperature caused an average loss of -50% for the 14-3-3 protein (range -32 to -91%) and -9% for tau. It is essential to ship CSF specimens frozen with dry-ice in order to obtain reliable results. Specimens should be shipped Monday through Thursday to avoid Saturday and holiday delivery. Supply Item Number 7211 Billing Code Test Code [sunquest] 17 HYDROXYCORTICOSTEROIDS, URINE 24HR 17OHQ 17OHQ Synonyms 17-OHcorticosteroids Container Type 24-hour leakproof plastic urine container Store and Transport Frozen Specimen Type 24-hour urine collection Preferred Volume 12 mL Minimum Volume 12 mL Collection Procedure Refrigerate during collection Specimen Processing Aliquot 12 mL of a well-mixed 24-hour urine collection into a leakproof plastic urine container and freeze. Record total volume. Required Patient Info Total volume and collection period Room Temp 4 hours with preservative Refrigerated 1 week with preservative Frozen (-20°C) 1 month Unacceptable Condition Alkali preservatives Alternate Specimens Random samples (but they are reported as mg/L with no reference ranges), samples refrigerated with preservatives. Sample pH must be 5-7. Mix well, add 1 gram boric acid/100 mL urine, adjust pH (with boric acid) to 5-7 and freeze. Reference Laboratory ARUP Reference Lab Test Code 70490 CPT Codes 83491 Test Schedule Tue, Fri Turnaround Time 4-7 days Method Quantitative Colorimetry Test Includes Collection Period, h; Volume, mL; 17-Hydroxycorticosteroids, mg/d; 17-Hydroxycorticosteroids, mg/gCr; Creatinine Urine, mg/dL; Creatinine Urine, mg/d Supply Item Number 1108 2.1 www.paml.com 4/16/2013 page 2 1 800.541.7891 509.755.8600 Fax 509.921.7107 Test Directory 1 Billing Code Test Code [sunquest] 17-HYDROXYPREGNENOLONE, LC/MS/MS 17HPG 17HPG Container Type Red top tube (plain) Store and Transport Refrigerated Specimen Type Serum Preferred Volume 2 mL Minimum Volume 0.4 mL Specimen Processing Separate serum from cells and put in separate plastic tube. Room Temp 24 hours Refrigerated 5 days Frozen (-20°C) 28 days Unacceptable Condition Received room temperature, serum separator tube (SST) Reference Laboratory Quest Diagnostics Nichols Institute (SJC) Reference Lab Test Code 8352X CPT Codes 84143 Test Schedule Mon, Thu Turnaround Time 4-6 days Method Liquid Chromatography/Tandem Mass Spectrometry Notes Infants with 21-hydroxylase deficiency may present with non-elevated results Supply Item Number 1372 Billing Code Test Code [sunquest] 17-HYDROXYPROGESTERONE 17HPRG 17HPRG Synonyms 17-OH Progesterone Container Type Serum separator tube (gold, brick, SST, or corvac) Specimen Type Serum Preferred Volume 0.5 mL Minimum Volume 0.2 mL Specimen Processing Separate serum or plasma from cells and place in separate plastic tube. Store and transport refrigerated or frozen. Room Temp 2 days Refrigerated 7 days Frozen (-20°C) 6 months Alternate Specimens EDTA or sodium heparinized plasma(lavender or green top tube). Department PSHMC Immunology Reference Laboratory PSHMC CPT Codes 83498 Test Schedule Mon, Wed, Fri evenings Turnaround Time 3-5 days Method RIA Test Includes 17-Hydroxyprogesterone, ng/dL. Supply Item Number 1467 Billing Code Test Code [sunquest] 17-HYDROXYPROGESTERONE, LC/MS/MS 17OHP 17OHP Container Type Red top tube (plain) Store and Transport Refrigerated Specimen Type Serum Preferred Volume 0.5 mL Minimum Volume 0.25 mL Specimen Processing Separate serum from cells and transfer to a standard PAML aliquot tube Room Temp 2 days Refrigerated 7 days Frozen (-20°C) 2 years Unacceptable Condition Samples collected in SST tubes. Do not submit glass tubes. Alternate Specimens Plasma; EDTA (lavender-top) , EDTA (royal blue-top), sodium heparin (green-top), or lithium heparin (green-top) Reference Laboratory Quest Diagnostics Nichols Institute (SJC) Reference Lab Test Code 17180 CPT Codes 83498 Test Schedule Sun-Fri Turnaround Time 3-4 days Method Liquid Chromatography Tandem Mass Spectrometry Supply Item Number 1372 2.1 www.paml.com 4/16/2013 page 3 1 800.541.7891 509.755.8600 Fax 509.921.7107 Test Directory 1 Billing Code Test Code [sunquest] 17-KETOSTEROIDS, URINE 24HR KETO 17KSUQ Container Type 24-hour dark plastic urine container Store and Transport Refrigerated Specimen Type 24-hour urine collection Preferred Volume 4 mL Minimum Volume 4 mL Collection Procedure Collect a 24-hour urine in a 24-hour dark plastic urine container. Refrigerate during collection. Specimen Processing Aliquot 4 mL of a well-mixed 24-hour urine collection into a leakproof plastic container. Record total volume and collection interval on transport tube and request form. Required Patient Info Record total volume and collection time interval on transport tube and request. Room Temp 4 hours Refrigerated 2 weeks Frozen (-20°C) 1 month Alternate Specimens 24 hour urine preserved with 6N HCl to a pH of 2-4. A pH of LT 2 will decrease analyte stability. Reference Laboratory ARUP Reference Lab Test Code 80650 CPT Codes 83586 Test Schedule Mon, Wed, Fri Turnaround Time 3-5 days Method Spectrophotometric (Zimmerman Reaction) Test Includes Time, h; Volume, mL; 17 Keto Steriods, mg/dL; 17 Keto Steroids, mg/d; Creatinine, Urine, mg/dL; Creatinine, Urine, mg/d Supply Item Number 1108 Billing Code Test Code [sunquest] 18-HYDROXYCORTICOSTERONE 18OHCC 18OHCC Separate samples must be submitted
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