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KPNW Specimen Requirements ***Please hit 'Ctrl'+'F' to open the 'Find on this page' funtion.

Panel Name Panel Description Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: GRN Li Hep or LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: 5 Days Test Schedule: Saturday Morning Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: 11-Deoxycortisol (Compound S) is useful in diagnosing patients with 11-beta- hydroxylase deficiency (second leading cause of congenital adrenal hyperplasia) and 11-Deoxycortisol primary (adrenal failure) or secondary (hypothalmic-pituitary ACTH deficiency) adrenal insufficiency.

Patient Preparation: An early morning specimen is preferred

Specimen Stability: Room Temperature: 4 days Refrigerated: 4 days Frozen: 4 weeks Ship serum frozen or refrigerated Test Code: CHANTILLY T.C.30543 TO SJC TC 30543X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Note if serum or plasma on specimen. Freeze solid. Transport: Transport frozen on ice.

Preferred Collection/Volume: Preserve 24-hour urine with 25 mL of 50% Acetic Acid (preferred) or 30 mL 6N HCl or 1 gram Boric Acid/100 mL (acceptable) during collection. Refrigerate during collection.

TAT: Next Day Test Schedule: Monday-Friday Night Method: Colorimetric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 17 Ketosteroids Total Pediatric 24 Hr Urine Labeling: Please specify on the request form and on the urine container the patient's age, sex, and (Includes Creatinine the total 24-hour urine volume Ratio) Specimen Stability: Room Temperature: 8 hours Refrigerated: 7 days Frozen: 30 days (-20c) Please specify on the request form and on the urine container the patient's age, sex, and the total 24-hour urine volume. Test Code: Quest TC 2796

Processing: Mix sample well and aliquot. Note on sample total volume and preservative used during collection. Transport: Refrigerate if held.

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Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 0.5 mL Serum

TAT: 3 Days Test Schedule: Monday-Friday, Sunday Night Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: 17-Hydroxyprogesterone is elevated in patients with congenital adrenal 17 OH-Progesterone hyperplasia (CAH). CAH is a group of autosomal recessive diseases, characterized by a deficiency of cortisol and an excess of ACTH concentration. 17-Hydroxyprogesterone is also useful in monitoring cortisol replacement therapy and in evaluating infertility and adrenal and ovarian .

Specimen Stability: Room Temperature: 48 Hours Refrigerated: 7 Days Frozen: 2 Years Test Code: SJC TC 17180

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: Preserve 24-hour urine with 25 mL of 50% Acetic Acid (preferred) or 30 mL 6N HCl or 1 gram Boric Acid/100 mL (acceptable) during collection. Preferred Specimen 20 mL 24-Hour Urine

TAT: Report available: Next Day Test Schedule: Monday-Friday Night Method: Colorimetric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 17- Hydroxycorticosteroids Specify on the request form and on the urine container the patient's age, sex, and the total 24-hour 24 Hour Urine urine volume. Transport refrigerated (cold packs). Specimen Stability: Room Temperature: 8 hours Refrigerated: 7 days Frozen: 30 days (-20c) Test Code: CHANTILLY T.C. 2796

Processing: Mix sample well and Aliquot 20 mL urine into sterile leak proof urine cup. Note on sample total volume and preservative used during collection. Transport: Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Report available: 6 Days Test Schedule: Monday, Thursday Night Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

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Clinical Data: 17-Hydroxypregnenolone is useful in the diagnosis of 3-Beta-Hydroxylase enzyme deficiency, a rare cause of congenital adrenal hyperplasia, and 17-Hydroxylase (P450c17) enzyme deficiency.

Specimen Stability: Room Temperature: 24 hours 17- Refrigerated: 5 days Hydroxypregnenolone Frozen: 28 days Test Code: SJC T.C.8352X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: 24 Hour Urine Container Volume: 100.0 mL 24-Hour Urine Instructions: Preserve with 25 mL 50% Acetic Acid (preferred) or 1 gram Boric Acid/100 mL (acceptable) during collection.

TAT: Report available: Next Day Test Schedule: Wednesday Evening Method: Colorimetric Test Facility: Quest Diagnostics Nichols Institute 17-Ketogenic Steroids 14225 Newbrook Drive Chantilly, VA 20153 24 Hour Urine Please specify on the request form and on the urine container the patient's age and sex and the total 24-hour urine volume. Transport at room temperature. Test Code: CHANTILLY T.C. 2806

Processing: Mix sample well and Aliquot 20 mL urine into sterile leak proof urine cup. Note on sample total volume and preservative used during collection. Transport: Send Room Temperature

Preferred Collection/Volume: Preserve 24-hour urine with 25 mL 50% Acetic Acid (preferred) or with 30 mL 6N HCl or 10 g Boric Acid (acceptable) during collection. 24 Hour Urine Container Volume: 20.0 mL of 24-Hour Urine

TAT: Report available: Next Day Test Schedule: Monday-Thursday, Sunday Evening Method: Colorimetric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 17-Ketosteroids Total 24 Hour Urine Please specify on the request form and on the urine container the patient's age and sex and the total 24-hour urine volume. Specimen Stability: Room Temperature: 8 hours Refrigerated: 1 week Frozen: 1 month (-20c) Test Code: CHANTILLY T.C. 2776

Processing: Mix sample well and Aliquot 20 mL urine into sterile leak proof urine cup. Note on sample total volume and preservative used during collection. Transport: Refrigerate if held.

Preferred Collection: Patient will present for collection between 9 and 13 weeks 6 days gestation.

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Volume: Filled blood spots on the NTD Laboratories filter paper card. NOTE: Obtain "NTD Laboratories" filter paper card previously completed by the provider for the specific patient in the lab's on-site files. If the form is not found in the on-site file, call Genetic Screening at PNS 503-296-1513 or 503-296-1500. They will get the form to the lab as quickly as possible.

Testing Location: NDT Laboratory 80 Ruland Road. Suite 1 Melville, NY 11747 Tel: 1-888-NTD-LABS (1-888-683-5227)

Clinical Data: First Trimester Screen | Fß test reliably assesses a mother's risk for having a baby with Down syndrome, trisomy 18 and 13 in the first trimester of pregnancy. Performed between 11 weeks, 1 day and 13 weeks, 6 days gestation, the First Trimester Screen | Fß uses data from a blood test (biochemistry) and an ultrasound examination (biophysical) which yields a risk assessment that physicians may present to their patients during the first trimester.

First Trimester Screen | Fß with Instant Risk Assessment enables trained medical professionals to present a complete risk assessment for Down syndrome and trisomy 18 and 13. Performed between 9 weeks and 13 weeks, 6 days of 1st Trimester Screen for gestation, the Instant Risk Assessment provides a care path for Down syndrome Downs Syndrome, risk assessment that is logistically simple to integrate within your overall prenatal Trisomy 18 care plan. In the earlier stages of the pregnancy (9 weeks to 11 weeks, 1 day), a dried blood sample obtained through a simple, safe finger stick method or venous blood may also be used to spot the dried blood spot card through the use of a Diff-Safe device. Dried blood sampling provides a more beneficial way to collect, store and process maternal blood specimens. Maternal blood samples are spotted onto an absorbent test card. This test card also functions as a hard copy data record of the patient's information including history, billing information, ultrasound dates and consent. After the blood spot collection is done, the cards are sent directly to NTD Labs where they are processed, tested and archived according to protocol. The results of the blood screen are maintained at NTD Labs and made available to the physician when the patient undergoes her NT (and optional fetal nasal bone assessment) ultrasound exam between 11 weeks, 1 day and 13 weeks, 6 days of gestation. To save time, NTD Labs provides web-based access to your patient's biochemistry data through a secure Instant Risk Assessment website. This site provides information management tools that allow you to call up individual patient records, enter key biophysical data from the ultrasound, and then present a complete risk assessment for Down syndrome and trisomy 18 and 13 at the time of the patient's visit. The calculation is based upon your patient's data entered on the spot, so there is less time spent between results reporting and patient notification.

SPECIMEN STABILITY: ROOM TEMP Ship STAT specimens overnight via Fed Ex.

Transport: Send Room Temperature

Preferred Collection/Volume: 24 hour urine collected with preservative Volume: 10.0 mL u

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Prepare sample container for 24-hour urine with 15 g Boric Acid or 25 mL 6N HCl to maintain a pH below 3. May be collected without preservative if pH is below 6 and the sample is shipped frozen upon receipt.

TAT: Report available: 2 Days Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Limitations Requires accurate 24-hour urine collection. Patients with renal disease may display falsely decreased results. Urinary 5-HIAA is increased in patients with malabsorption disorders who also display a larger concentration of urinary metabolites. Urinary 5-HIAA is increased in patients with intestinal obstruction and with some noncarcinoid islet cell 5 HIAA 24 Hour Urine tumors. Carcinoid syndrome may not cause elevated results, especially if the patient does not have diarrhea.

Patient Preparation: Patient should avoid food high in indoles: avocado, banana, tomato, plum, walnut, pineapple, and eggplant. Patient should also avoid , tea, and coffee for 3 days before specimen collection.

Record patient's age and total volume on the test request form and on the urine container. Specimen Stability: Room Temperature : 1 week Refrigerated: 1 month Frozen : 1 month TC # 2636

Processing: Mix sample well and aliquot 10 mL urine into sterile leak proof urine cup. Note on sample total volume and preservative used during collection. Transport: To AWL in blue tote.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL Serum Alternative Collection: RED SST Volume: 1.0 mL serum

TAT: Report available: Next Day Test Schedule: Mon-Fri All Shifts Sunday Night Method: Colorimetric, Kinetic Test Facility: Quest Diagnostics Nichols Institute 5' Nucleotidase 14225 Newbrook Drive Chantilly, VA 20153

Specimen Stability: Room temperature: Unacceptable Refrigerated: 7 days Frozen: 14 days Test Code: CHANTILY T.C. 671Z

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze solid. Transport: Transport frozen on ice.

Preferred Collection/Volume: 5 mL whole blood collected in an EDTA (lavender-top) tube Whole blood: Normal phlebotomy procedure Alternative Collection: Whole blood collected in EDTA (royal blue-top), Sodium heparin

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(green-top), Lithium heparin (green-top), ACD solution B (yellow-top) or ACD solution A (yellow- top) • Extracted DNA

TAT: Report available: 4-5 days Test Schedule: Set up: Mon, Wed, Fri Method: Polymerase Chain Reaction (PCR) • Restriction Digestion • GeneScan Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Individuals who carry two copies (homozygous) for the Z allele are at a higher A-1-AT Mut risk to develop liver disease and emphysema.

Specimen Stability: Room temperature: 8 days Refrigerated: 8 days Frozen: Unacceptable Test Code: SJC TC 15340X

Processing: Do Not Centrifuge: Specimen stability is crucial. Store and ship room temperature immediately. Do not freeze. Transport: Send Room Temperature

Preferred Collection/Volume: Pink EDTA Volume: 5.0 mL Alternative Collection: LAV EDTA RED 10 (do not centrifuge) ABO-RH Absolute Minimum Volume: 1.0 mL

For routine ABO, RH only. ABO & RH included in Type & Screen and RHIG.

Preferred Collection/Volume: Pink EDTA Volume: 5.0 mL Alternative Collection: LAV EDTA RED 10 (do not centrifuge) Absolute Minimum Volume: 1.0 mL

ABORH Type and Specimen must be coll within three days of surgery or possible transfusion. Screen Profile Blood and Blood Component Requisition must accompany specimen. Attach the extra label marked "FORM" to the requisition in the blank area to the right of the identifier signature. The form label must be dispatched and received just like the specimen. Identification signatures required. Refer to SOP#2.1-18-200 Blood Bank Pretransfusion Orders, Specimen and Patient Identification.

Preferred Collection/Volume: Pink EDTA Volume: 5.0 mL Alternative Collection: LAV EDTA RED 10 (do not centrifuge) Absolute Minimum Volume: 1.0 mL ABORH Type Antibody Screen Profile (For Blood and Blood Component Requisition must be sent with specimens. Attach the Crossmatch) extra label marked "FORM" on the requisition in the blank area to the right of the identifier signature. The form label must be dispatched and received just like the specimen. Identification signatures required. Refer to SOP#2.1-18-200 Blood Bank Pretransfusion Orders, Specimen and Patient Identification.

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Preferred Collection/Volume: RED 10 Volume: 3mL s Absolute Minimum Volume: 1.0mL s

Test Schedule: Performed once per week. Test Facility: OHSU Mail code BTE355 3181 S.W. Sam Jackson Park Road Portland, OR 97239 ABPA Panel Allergic Bronchopulmonary Aspergillosis (ABPA) Profile Clinical Data: This profile combines four of the seven criteria for the diagnosis of ABPA. Tests included are: Total serum IgE, Aspergillus fumagatis specific IgE, Aspergillus specific IgG along with a panel of precipitins against twelve Aspergillus Antigens

Transport refrigerated to testing lab. TC# 064-0945

Transport: Transport on ice.

Preferred Collection/Volume: Sterile leakproof container/>0.5 mLs Alternative Collection: Liquid Amies Transport Swab (E-swab): If fluid is <0.5 mL, place in Liquid Amies Transport Swab (E-swab). Syringe without needle.

TAT: 5 - 10 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: Specific source must be provided. If specimen is a sterile cavity body fluid, refer to body fluid culture for information. Abscess/Aspirate Patient History: n/a Culture, Aer and Ana Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired transport media. Greater than 24 hours post collection (non-swab specimen). Greater than 48 hours post collection (E-Swab). Syringe with needle attached.

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: Minimum of 2 mL EDTA whole blood Alternative Collection: n/a

TAT: Stat one hour; routine same day Test Schedule: Any day Method: Hematology Cell counter or manual differential Absolute Neutrophil Test Facility: All KPNW labs with hematology testing. Count Note : ANC is a calculated parameter which is derived from the WBC and % neutrophil counts.

Clinical Data: n/a Patient History: n/a

Patient Preparation : n/a

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Labeling: n/a

Processing: Handle microtainer ASAP and test on-site when possible. Transport: Transport at refrigerator temperature when sending to AWL.

Preferred Collection: DK GRN Li Hep Alternative Collection: RED 10 EDTA Volume: 1.0 mL plasma/serum (Absolute Minimum Volume: 0.2mL plasma/ serum) Note: No gel barrier tubes

TAT: One hour Acetaminophen Level Testing Schedule: Daily Method: Testing Location: Sunnyside Medical Center and Westside Medical Center

Clinical Data: Suspected overdose preferred drawtime is 4 and 12 hrs after ingestion.

Processing: Centrifuge, aliquot, refrigerate.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: 3 Days Test Schedule: Monday-Friday Morning Method: Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Myasthenia gravis (MG) is a neuromuscular disorder, characterized by muscle weakness, most commonly due to autoantibody-mediated loss of functional acetylcholine receptors Acetylcholine Receptor (AChR) in the neuromuscular junction. This assay aids in the differential diagnosis of MG-like Binding Antibody muscle weakness, in differentiating between generalized MG and ocular MG, and in monitoring therapeutic response. If binding are negative, blocking and modulation antibodies should be considered. See Myasthenia Gravis and Acetylcholine Receptor Antibodies in the chapter, Interpretive Information section.

Specimen Stability: Room Temperature: 14 days Refrigerated: 14 days Frozen: 1 year Test Code: CHANTILLY T.C. 206

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: Amniotic Fluid in a sterile container Volume: 1.5 mL

TAT: Report available: Next Day Test Schedule: Tues, Thurs evening Method: Gel Electrophoresis Radial AFP: Chemiluminescent Spectrophotometric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive

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Chantilly, VA 20153

Clinical Data: The presence of acetylcholinesterase (AChE) activity in amniotic fluid is associated with open neural tube defects. The AChE assay is useful in assessing the evaluation of a positive AFP result and in evaluating amniotic fluid samples with elevated AFP concentrations. There is indication that the AChE assay exhibits fewer false positives than the AFP assay. The two assays together are complimentary in the clinical assessment of neural tube defects. Acetylcholinesterase Indicate gestational age, collection date, clinical indications and the AFP and MOM results, if Amniotic Fluid available, on the test requisition. Specimen Stability Room Temperature: 14 Days Refrigerated: 4 Months Frozen: 3 Years

Transport: Refrigerate if held

Preferred Collection/Volume: Avoid hemolysis and/or lipemia. Preferred Specimen: 2 LAV EDTA Volume 5.0 mL whole blood and 1.0 mL Plasma

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning Method: Kinetic Spectrophotometric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Approximately 1 in every 2500 individuals has inherited defective or deficiency of the enzyme (pseudocholinesterase) that metabolizes succinylcholine (an anesthetic agent). With normal dosage, these individuals have prolonged apnea. Such individuals are responsive at much smaller concentrations of this anesthetic agent than the general population. Low concentrations of Pseudocholinesterase are observed in individuals exposed to organophosphorous insecticides and Acetylcholinesterase patients with hepatic dysfunction. Red Blood Cell Rejection Criteria : Received room temperature; Received frozen; Hemolyzed plasma; Receipt of only a single tube. Ship both whole blood and plasma samples refrigerated in the same shipping container. Do not send packed cells. Specimen Stability: Room Temperature 24 hours Refrigerated: 7 Days Frozen: Unacceptable Test Code: CHANTILLY T.C. 4091

Processing: Centrifuge one tube to separate plasma. Aliquot plasma into referred test aliquot tube. Transport both whole blood and plasma together. Transport: Transport on ice.

Preferred Collection/Volume: Sputum : Recommend collecting 3 early morning specimens. Minimum specimen volume is 3 mL. Place each specimen into a separate sterile leakproof container. BAL/Bronch Wash : Minimum specimen volume is 3 mL. Place specimen into a sterile leakproof container. Blood : Inoculate 3 to 10 mL blood into AFB Blood culture bottle (Yellow SPS) Bone Marrow : Inoculate bone marrow into SPS tube; SPS tube must be completely filled with specimen. Body Fluids : Minimum specimen volume is 1mL. Place specimen into a sterile leakproof container. Tissue : Greater than 1 mm of tissue placed in sterile leakproof container; add a small amount of sterile saline to keep specimen moist. Urine: First morning midstream urine. Place specimen in sterile leakproof container. Minimum

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volume is 3 mL. Abscess/Aspirate : Place specimen into a sterile, leakproof container. Alternative Collection: Liquid Amies transport swab (E-swab): Tissue or open wound material may be submitted using an E-swab

TAT: AFB Smear: within 24 hours AFB Culture: 6 - 8 weeks Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: n/a Patient History: n/a

Patient Preparation: Collect specimens first thing in the morning. For 3 tests, you will need to collect a specimen early in the morning 3 days in a row. Rinse mouth and gargle with water; NOT mouthwash. Cough deeply. Screw lid on tightly to container provided by the laboratory. Acid Fast Bacilli Labeling: If patient is collecting specimen at home, instruct them to clearly label collection date Culture and time on each container.

Rejection Criteria: Greater than 5 days post collection Dehydrated specimens Additive in sterile container (i.e. formalin, alchohol) Sterile body fluid received on a swab Rectal Swab 24 hour pooled urine or sputum SPS tube less than maximum fill CSF less than 2 mLs Sterile body fluid less than 1 mL Expired collection/transport device

Processing: Blood and Bone Marrow - Hold at room temperature. All others - Refrigerate Transport: Blood and Bone Marrow - transport at room temperature. All others - Refrigerate

Preferred Collection: Red SST Alternative Collection: RED 10 Volume: 1.0 mL Serum Minimum Volume: 0.5 mL

TAT: Report available next day Test Schedule: Monday-Friday Afternoon Test Method: Immunoassay Acid Phosphatase Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: PAP measurement has found clinical application in the management of prostatic cancer patients. Serum PAP measurements have been useful in monitoring remission or relapse of a prostatic malignancy and in assessing the effectiveness of various treatment regimes. Thus, normalization in serum PAP levels has been observed following successful therapeutic intervention, while recurrent or residual disease has been associated with elevated levels of PAP.

Patient Preparation: Clinician Instructions ONLY: Samples should be obtained before rectal examination, biopsy, prostatectomy, or prostatic massage, since manipulating the prostate gland may lead to elevated PAP levels persisting up to

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24-48 hours.

Specimen Stability: Room Temperature: 24 hours Refrigerated: 24 hours Frozen: 1 month Rejection Criteria: Reject unfrozen specimens if received more than 24 hours after collection. TC# 7812

Processing: Centrifuge and aliquot into Referred Tests aliquot tube Freeze solid Transport: Transport frozen on ice.

Preferred Collection/Volume: LAV EDTA Volume: 1.0 mL p

TAT: 24 Hours Test Schedule: Daily ACTH Method: Chemiluminscent Immunoassay Test Facility: AWL

Processing: Centrifuge and aliquot as soon as possible. Freeze. Transport: Transport frozen on ice. Do not allow specimen to thaw.

Preferred Collection/Volume: BLUE CIT Note:Tube must be filled to minimum fill indicator

TAT: 2-7 days Test Schedule: Once per week Method: Clot based Testing Location: Airport Way Laboratory Activated Protein C Patient Preparation: If using a butterfly, a citrate discard tube must be used first, Resistance when citrate is the first tube collected.

Processing: Centrifuge immediately and aliquot 1.0 mL plasma into 2 aliquot tubes using a plastic pipette. DO NOT USE FILTERS. Avoid buffy coat. Platelet poor plasma required. Freeze solid. Note: Aliquots may be shared with Protein C or Protein S Transport: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection/Volume: Brucella:RED 10 Volume: 2 mL Serum Leptospirosis:RED 10 Volume: 2 mL Serum Tularemia: RED 10 Volume: 2 mL Serum Rickettsia: RED 10 Volume: 10 mL Serum

TAT: Brucella: 1-6 days Leptospirosis: 1-6 days Tularemia: 1-6 days Ricketsia:2 - 7 working days Test Schedule: Brucella: Weekly Leptospirosis: Weekly Tularemia: Weekly Rickettsia: Weekly Acute Method: Brucella: Bacterial

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Infectious Disease Leptospirosis:Indirect (IHA) Tularemia:Bacterial Agglutination Rickettsia:Indirect Assay (IFA) Test Facility: Oregon State Lab 3150 NW 229th Ave., Suite 100 Hillsboro, OR 97124-6536

Patient History: Required Information: Date of onset , and list which of the following viral serology tests: Brucella, Leptospirosis, Tularemia, or Rickettsia Panel.

Specimen Stability: Refrigerated only

Processing: Centrifuge and aliquot serum into Referred Tests aliquot tube Transport: Transport on ice.

Preferred Collection/Volume: Avoid hemolysis GRN NA HEP 6 Volume: 1.0 mL Plasma

TAT: Report available: 6 Days Test Schedule: Tuesday, Thursday, Saturday Morning Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Elevation of one or more acylcarnitines is diagnostic for an organic aciduria or a fatty disorder. A low Carnitine level may mask a disease state. Acylcarnitines Quantitative Patient Preparation: Patient should be in a well-fed state. Labeling: Date of birth is required

Specimen Stability: Room Temperature: 1 Hour Refrigerated: 1 Hour Frozen: 30 Days Test Code: CHANTILLY T.C. 14531 TO SJC TC 14531

Processing: Centrifuge immediately and aliquot into Referred Tests aliquot tube. Freeze solid. Transport: Transport frozen on ice

Preferrred Collection/Volume: Pleural fluid submitted in sterile leakproof container. Volume: 0.5 mL Absolute Minimum Volume: 0.1 mL

TAT: Next Day Test Schedule: Monday-Friday Test Method: Kinetic • Spectrophotometric Testing Location: Performing Laboratory Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Significance An elevated level of adenosine deaminase (AD) in human serum, plasma or select body

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fluids is used as a surrogate marker for inflammation or infectious disease. In CSF, pleural effusions, and pericardial fluid in particular, elevated AD activity has been shown to be associated with tubercular lesions. Additional disease states that may result in elevated AD activity include liver cirrhosis, liver fibrosis, certain malignancies (e. g. lymphoma), viral hepatitis, and autoimmune diseases (e. g. SLE, rheumatoid arthritis).

Transport Temperature Frozen Adenosine Deaminase Pleural Fluid Specimen Stability Room temperature: 24 hours Refrigerated: 72 hours Frozen: 6 months TC# 17697

Processing: Freeze sample upon receipt Transportation: Transport to AWL on ice

Preferred Collection/Volume: Collect blood in a prechilled Preferred Specimen LAV EDTA, pre-chilled LAV EDTA Volume: 4.0 mL Plasma

TAT: Report available: 6-7 Days Test Schedule: Monday, Wednesday, Friday Morning Method: Extraction Radioimmunoassay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Antidiuretic Hormone (also called ADH or Vasopressin) regulates water ADH Level reabsorption in the kidney, reducing diuresis and increasing blood volume and pressure. The syndrome of inappropriate release of ADH has been labeled SIADH, occurring with neoplasia, pulmonary disorders (e.g., pneumonia and tuberculosis), CNS disorders, and with specific drugs.

Specimen Stability: Room Temperature: 1 Hour Refrigerated: 6 Hours Frozen: 6 Months Test Code: SJC T.C. 252X

Processing: Centrifuge immediately. Aliquot into Referred Tests aliquot tube. Freeze immediately. Do not thaw. Transport: Transport frozen on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Report available: 3 Days Test Schedule: Tuesday-Friday Morning Adrenal Antibody Method: Indirect Immunofluorescence Assay Screen Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: If Adrenal Antibody Screen is positive, Adrenal Antibody Titer with Pattern will be performed at an additional charge (CPT code(s): 86256).

Specimen Stability:

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Room temperature: 48 hours Refrigerated: 14 days Frozen: 30 days Test Code: CHANTILLY T.C. 8292

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: Blood : Yellow SPS Tube Volume: 3-10mL wb

TAT: AFB Smear: within 24 hours AFB Culture: 6 - 8 weeks AFB Blood Test Schedule: Daily Method: Culture Test Facility: Airport Way Regional Laboratory

Processing: Keep Room Temperature Transportation: Transport to AWL in Red Tote-Room Temperature

Preferred Collection/Volume: Pure culture (safely contained in a double-walled container) In order to perform susceptibility testing to the drugs of choice and to interpret test results, the identification of the isolate is required. Transport as indicated. Pure Isolate of AFB

TAT: Report available: 30 Days Test Schedule: Monday, Tuesday, Thursday Morning Method: Agar proportion Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave, Suite 200 AFB Susceptibility, Cypress, CA 90630 Clarithromycin Patient Preparation: Clinician Instructions ONLY : In order to perform susceptibility testing to the drugs of choice and to interpret test results, the identification of the isolate is required.

Specimen Stability: Room Temperature: viable organism Refrigerated: viable organism Frozen: unacceptable Test Code: CHANTILLY T.C. 19585X TO FOCUS TC 52043

Transport: Transport on ice.

Preferred Collection/Volume: Red 10 No Gel Barrier Tubes Volume: 2.0 mL s

TAT: 3 days Test Schedule: M-W-F Method: Immunoassay AFP Maternal Test Facility: AWL

Clinical Data: Testing done only at 15-20 weeks gestation.

Processing: Centrifuge and aliquot. Transport: Refrigerate if held overnight.

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Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p Alternative Collection: Red SST Volume: 0.2 mL s

TAT: 24 hours Albumin Level Test Schedule: Daily Method: Photometric, Bromcresol Green Test Facility: AWL, INT, SMC, LVK

Processing: Centrifuge. Refrigerate if held.

Preferred Collection/Volume: GRN Li Hep Volume: 4.0 mL Body Fluid Green tube draws 3mL, fill to short line Alternative Collection: Red 10 Volume: 1.0 mL

Albumin Level Body TAT: 24 hours Fluid Test Schedule: Daily Method: Chemiluminscent Immunoassay Test Facility: AWL, INT, SMC, LVK

Processing: Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Report available: Next Day Test Schedule: Mon-Fri Morning, Afternoon, Night Method: Enzymatic Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Aldolase Clinical Data: Aids in the diagnosis of primary disease of skeletal muscle myocardial infarction and viral hepatitis.

Specimen Stability: Room temperature: Not established Refrigerated: 5 days Frozen: 14 days Test Code: CHANTILLY T.C. 0212

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze solid. Transport: Transport frozen on ice

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: 3 Days Test Schedule: Monday, Wednesday, Friday Evening Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

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Clinical Data: Approximately 1-2% of individuals with primary hypertension have primary hyperaldosteronism, characterized by hypokalemia (low potassium) and low direct renin. Because serum aldosterone concentrations vary, due to dietary sodium intake and body position, some physicians prefer measurement of 24-hour urine concentrations for aldosterone.

Patient should be in an upright sitting position at least one half hour prior to colllection.

Rejection Criteria: Aldosterone Specimen received in glass tube or in a serum separator. Specimen Stability: Room Temperature: 4 days Refrigerated: 7 days Frozen: 28 days Test Code: CHANTILLY T.C. 53532

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: 24-Hour Urine collection with 10 gms boric acid to maintain pH < 7.5. Refrigerate during collection. Preservative may be added after collection if other urine testing is requested. Volume: 20mL u Minimum Volume : 5.0 mL u

TAT: Report available: 5 Days Test Schedule: Tuesday, Thursday, Sunday Night Method: Hydrolysis, Extraction, Radioimmunoassay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano Aldosterone 24 Hour 33608 Ortega Highway Urine San Juan Capistrano, CA 92690-6130 Specimen Stability: Room Temperature: 2 days Refrigerated: 1 week Frozen: 2 years Test Code: SJC T.C. 19552X

Processing: Mix sample well and aliquot 20 mL urine into sterile leak proof urine cup. Record total volume and preservative used on sample. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 1.8 mL plasma

TAT: Report available: 2-3 days Test Schedule: Monday-Thursday, Sunday night Method: Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS) Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano Aldosterone and Renin 33608 Ortega Highway Levels w Ratio San Juan Capistrano, CA 92690-6130

Clinical Data: The Aldosterone/Renin Ratio is used to screen for primary aldosteronism.

Specimen Stability: Room Temperature: 24 hours Refrigerated: unacceptable Frozen: 28 days Test Code: SJC T.C. 16845

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Processing: Centrifuge and aliquot plasma into Referred Tests aliqout tube. Freeze. Refrigeration will cause cryoactivation to occur and prorenin will convert to renin, causing falsely high renin results. DO NOT REFRIGERATE THE SAMPLE. Transport: Transport frozen on ice

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p Alternative Collection: RED SST Volume: 0.5mL s

TAT: One hour STAT - 24 Hours for Routine Alkaline Phosphatase Test Schedule: Daily Method: photometric, p-Nitrophenol Phosphate Test Facility: SMC, INT, BVT, AWL, CPK, LVK

Processing: Centrifuge. Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Friday, Sunday Evening Method: Electrophoresis Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Measurement of Alkaline Phosphatase Isoenzymes may be useful in determining the source of increased Total Alkaline Phosphatase activity and may assist in the diagnosis of Alkaline Phosphatase hepatobiliary disorders and bone disease associated with increased osteoblastic activity. Isoenzymes Patient History: Test includes: Alkaline Phosphatase, Intestinal Isoenzymes, Bone Isoenzymes, Liver Isoenzymes, Placental Isoenzymes, Macrohepatic Isoenzymes

Patient Preparation: Overnight fasting is preferred.

Specimen Stability: Room Temperature: 7 days Refrigerated: 21 days Frozen: 21 days Test Code: CHANTILLY T.C. 6432

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Almond IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours

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Test Schedule: Mon - Fri Method: Immunoassay Test Facility: AWL Allergen, Brazilnut IgE Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Cashew IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Corn IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Egg White IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Egg Yolk IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Hazelnut IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

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Preferred Collection/Volume: Red Top Tube/1.0mL Alternative Collection: Red Top Tube/0.3mL

TAT: 24 hours Test Schedule: Monday - Friday Allergen, Latex Method: Immunoassay Test Facility: AWL

Processing: Centrifuge. Aliquot.

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Milk IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Peanut IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Pecan IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Pistachio IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s Allergen, Shrimp IgE TAT: 24 Hours Test Schedule: Mon - Fri Method: Immunoassay

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Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Soybean IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Walnut IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: / 0.3 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Allergen, Wheat IgE Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: Collect additional RED 10 if needed to assure appropriate amount of serum is submitted for testing. RED 10 Volume: .5 mL Serum needed for each allergen.

Test Facility: Quest Diagnostics, Chantilly, VA

Allergens, Rast, Patient Preparation: Specific Allergens must be listed within the order Multiple (Qual) For latex allergens see LTX panel.

Ensure Allergens are listed in result field. Send with Manual Reguisition.

Processing: Centrifuge and aliquot serum into a referred tests aliquot tube. If sending more than one aliquot, rubberband together. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0mL Serum Alternative Collection: LAV EDTA Volume: 2. mL Plasma

TAT: Wednesday 3 days Test Schedule: Wednesday Morning

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Method: High Performance Liquid Chromatography Test Facility: NMS LABS 3701 Welsh Road Willow Grove, PA 19090

Clinical Data: Therapeutic drug monitoring (scope: Allopurinol and Oxipurinol)

Allopurinol Level, Specimen Stability: HPLC Room Temperature: Undetermined Refrigerated: Undetermined Frozen (-20 °C): Undetermined Test Code: CHANTILLY TC 111211 TO NMS TC 0213SP

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Note if serum or plasma on specimen .

Preferred Collection/Volume: Red Top Tube/1.0mL serum NO GEL BARRIER TUBES Alternative Collection: Red top tube/0.3mL

TAT: 24 hours Test Schedule: Daily Alpha Fetoprotein Method: Immunoassay Test Facility: AWL

Clinical Data: Test for males and non-pregnant females only. If pregnant see panel MSAFP4 screen, For CSF see panel Alpha Fetoprotein Tumor Marker, CSF.

Processing: Centrifuge. Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Report available: 2 days Test Schedule: Set up: 2 days a week, a.m Method: Radioimmunoassay Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Alpha Subunit of Clinical Data: This assay is useful in the diagnosis and management of various pituitary, Pituitary Glycoprotein placental, pancreatic and hCG-producing tumors. The assay can also be useful for investigations Hormone where alpha subunit is increased under various physiological stimuli (e.g.,) GnRH stimulation and TRH stimulation tests.

Specimen Stability: Room temperature: 7 days Refrigerated: 7 days Frozen: 28 days Test Code: SJC TC 8658X

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Regfrigerate if held

Preferred Collection/Volume: CSF Sterile container/1.0mL Alternative Collection: Preferred tube number for CSF collections is #1, but any tube may be used/ 0.3mL

TAT: 24 hours Alpha-1 Fetoprotein, Test Schedule: Daily

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Tumor Marker, CSF Method: Immunoassay Test Facility: AWL

Processing: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume : 1.0 mL s Absolute Minimum Volume: .5mL s

TAT: Next Day Test Schedule: Monday-Saturday Afternoon Method: Nephelometric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Patient Preparation: Overnight fasting is preferred.

Clinical Significance Alpha-1-Antitrypsin Alpha-1-Antitrypsin level may be increased in normal pregnancy and in several diseases including chronic pulmonary disease; hereditary angioedema; renal, gastric, liver and pancreatic diseases; diabetes; and rheumatoid diseases. Alpha-1-Antitrypsin may be decreased in emphysema, hepatic cirrhosis, respiratory distress syndrome of the newborn, nephrosis, malnutrition and cachexia.

Specimen Stability: Room Temperature: 2 days Refrigerated: 1 week Frozen : 3 months (-20c) TC# 4482

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Regfrigerate if held

Preferred Collection/Volume: RED 10 (protect from light) No Gel Barrier Tubes Volume: 2.0 mL Serum Wrap collected sample in foil to protect from light.

TAT: Report available: 4 Days Test Schedule: Monday, Wednesday, Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Alprazolam Level Specimen Stability: Room Temperature: 5 days Refrigerated: 14 days Frozen: 1 year Test Code: CHANTILLY TC 21592

Processing: Foil wrap specimen to protect from light. Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: GRN Li Hep PST Volume 0.5mL p Alternative Collection: RED SST Volume: 0.2mL s

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TAT: 24 hours Test Schedule: Daily Method: Photometric rate, L-Alanine with Alpha-Oxoglutarate ALT Test Facility: SMC, INT, BVT, AWL, CPK, LVK

Processing: Centrifuge. Refrigerate if held.

Preferred Collection/Volume: Draw one Dark Blue and discard. Draw second Dark Blue No Additive. Preferred Specimen: Dark Blue Serum Volume: 2.0 mL Serum

TAT: Report available: 3 Days Test Schedule: Monday, Wednesday, Friday Afternoon Method: Atomic Spectroscopy Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Aluminum Level Chantilly, VA 20153 Specimen Stability: Room Temperature: 4 days Refrigerated: 14 days Frozen: 30 days Test Code: CHATNILLY TC 2958

Processing: Allow sample to clot in an upright position. Centrifuge and pour (do not pipette) the serum into Referred Tests aliquot tube. Transport: Refrigerate if held.

. Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: 24 hours Test Schedule: 7 days/week Method: Enzymatic Immunoassay Test Facility: Legacy Hospital Laboratory 2801 N Gantenbein Portland, OR 97232

Clinical Data: Treat all Amikacin specimens as STAT. Amikacin Level Patient Preparation: Daily Dosing; Random: 8-12 hours after dose, Trough: 30 minutes before dose

Specimen Stability: Room Temperature: 4 hours Refrigerated: 24 hours Frozen (at or below -20°C): 1 month LEGACY CS: 503-413-1234.

Processing: Centrifuge and remove serum or plasma from cells ASAP. Allow serum to clot completely at room temperature (minimum: 30 minutes).Note if serum or plasma on specimen. Cab directly with Legacy Lab Requisition and Specimen Transport Form 23-09-204. CALL LEGACY CS 503-413-1234 for delivery instructions prior to sending Transport: Transport on ice.

Preferred Collection/Volume: Green NA or Li Hep *No Gel Barrier*

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Volume: 3mL p Pediatric Volume: .5 mL p

Test Facility: OHSU Laboratories Dillehunt Hall room 3020 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Clinical Data: Indication/description: Confirmation of abnormal screen, definitive diagnosis, Amino Acid monitoring of treatment, etc. Quantitative Patient History: List clinical indication and all medications from previous 24 hr.

Specimen Stability: Frozen only Test Code: OHSU TC 067-6310

Processing: Centrifuge and aliquot into a Referred Tests aliquot tube. Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: 2 mL random urine or aliquot of a 24 hr urine.

Test Facility: OHSU Laboratories Dillehunt Hall room 3020 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Clinical Data: Indication/description: Confirmation of abnormal screen, definitive diagnosis, Amino Acid monitoring of treatment, etc. Patient History: List clinical indication and all medications from previous 24 hr. Quantitative Urine Specimen Stability: Frozen Test Code: OHSU TC 067-6311

Processing: Mix 24 hour sample well and aliquot. Note on sample total volume. Freeze random or timed specimen Transport: Transport frozen on ice.

Preferred Collection/Volume: 8 mL random urine (first morning sample preferred but not essential) or aliquot of 24 hr collection. Refrigerate and deliver within 4 hr or freeze Min Volume: 5mL

Test Facility: OHSU Laboratories Dillehunt Hall room 3020 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Clinical Data: NOTE: This is the preferred test for initial screening for inborn errors of metabolism since it covers a WIDE range of abnormalities. Amino Acid Screen Indication/description: Includes Ketoacids, Ketones, Sulfur Aminoacids, Mucopolysaccharides, Urine Urobilinogen, Porphobilinogen, Bilirubin, Glucose, Protein and Reducing Substances. Patient History: List clinical indication, and all medications from previous 48 hr

Specimen Stability: Frozen Test Code: OHSU TC 067-6301

Processing: Mix 24 hour sample well and aliquot. Note on sample total volume. Freeze random or timed specimen Transport: Transport frozen on ice.

Preferred Collection/Volume: Green NA HEP

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Volume: 3 mL Plasma Min Volume 1mL

Test Facility: OHSU Laboratories Dillehunt Hall room 3020 3181 S.W. Sam Jackson Park Road Portland, OR 97239 Amino Acid Single Quantitative Specimen Stability: Frozen Test Code: OHSU TC 067-6313

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 3.0 mL Serum

TAT: Report available: 2-3 days Test Schedule: Set up: 4 days a week a.m Method: Liquid Chromatography • Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Amitriptyline is an antidepressant commonly used for treating depression, pain of Amitriptyline and neuropathic origin, attention-deficit hyperactivity disorder, functional enuresis in children, and panic Nortriptyline Levels and phobia disorders. Therapeutic drug monitoring is useful to optimize dose and avoid toxicity. Patient Preparation: Optimum time to collect sample: 10-14 hours post oral dose

Specimen Stability: Room temperature: 48 hours Refrigerated: 7 days Frozen: 30 days Test Code: SJC TC 638N

Processing: Separate from cells as soon as possible after clotting. Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection: GRN Li Hep Alternative Collection: LAV EDTA Volume: 1.5mL plasma (Absolute Minimum Volume: 0.5mL plasma) Note: No gel barrier tubes. Place speicmen in ice bath immediately after collection.

TAT: STAT Testing Schedule: Daily Method: Colormetric Ammonia, Arterial Testing Location: Sunnyside Medical Center and Westside Medical Center Rejection Criteria: Gross Hemolysis

Note: The laboratory does not draw arterial samples. Contact Respiratory Therapy department for collection of arterial samples.

Processing: Medical Center Campus Locations: Centrifuge and aliquot within 15 minutes of collection. Cap aliquot immediately. Deliver to testing bench immediately, in ice bath.

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Medical Office Locations: Centrifuge and aliquot within 15 minutes of collection. Cap aliquot immediately. Freeze. Transport: Medical Center Campus Locations: Deliver sample in ice bath to laboratory within 15 minutes of collection. Medical Office Locations: Transport frozen on ice.

Preferred Collection: GRN Li Hep Alternative Collection: LAV EDTA Volume: 1.5mL plasma (Absolute Minimum Volume: 0.5mL plasma) Note: No gel barrier tubes. Place speicmen in ice bath immediately after collection.

TAT: STAT Testing Schedule: Daily Method: Colormetric Testing Location: Sunnyside Medical Center and Westside Medical Center Rejection Criteria: Gross Hemolysis Ammonia, Venous Processing: Medical Center Campus Locations: Centrifuge and aliquot within 15 minutes of collection. Cap aliquot immediately. Deliver to testing bench immediately, in ice bath. Medical Office Locations: Centrifuge and aliquot within 15 minutes of collection. Cap aliquot immediately. Freeze. Transport: Medical Center Campus Locations: Deliver sample in ice bath to laboratory within 15 minutes of collection. Medical Office Locations: Transport frozen on ice.

Preferred Collection/Volume: Amniotic Fluid in sterle leak proof conatiner Volume: 1.0-20.0 mL Aminotic Fluid

TAT: Preliminary Negative: 1 day; Final Negative: 5 days Test Schedule: 7 days/week Method: Standard Aerobic Bacterial Culture, Anaerobic Culture performed on properly collected specimens Test Facility: Legacy Central Laboratory 1225 NE 2nd Ave Portland, OR 97232 Amniotic Fluid Panel Patient Preparation: Clinician Instructions ONLY: Decontaminate site with scrub/prep, use aseptic collection during aspiration.

Ambient: 24 hours

Processing: Treat all specimens as STAT. ransport refrigerated on ice within 2 hours of collection. Cab directly to Legacy Central Lab with completed Legacy Requisition and Specimen Transport Form 23-09-204. Include gestational age, # fetuses, and if mother is diabetic on Legacy Requistion. Dispatch Specimen to AWL. Transport: Send Room Temperature

Preferred Collection/Volume: 2 mL Serum or Plasma

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TAT: 2 days Test Schedule: Monday 2nd Shift Method: High Performance Liquid Chromatography Test Facility: NMS LABS 3701 Welsh Road Willow Grove, PA 19090

Clinical Data: Therapeutic Drug Monitoring

Amoxapine Level NOTE: Test performed at NMS via Quest Chantilly. Specimen Stability: Room Temperature: 7 day(s) Refrigerated: 14 day(s) Frozen (-20 °C): 10 month(s) Test Code: Quest TC 885

Processing: Centrifuge and aliquot into a Referred Test aliquot tube.Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: Sterile Urine Cup/ 50mL urine Alternative Collection: 10mL urine

TAT: 3 days Test Schedule: Monday-Wednesday-Friday Conf Method: GC/MS Urine Test Facility: AWL

Reflexed from Amphetamine screen.

Processing: Refrigerate if held overnight.

Preferred Collection/Volume: Sterile Urine Cup/ 50mL urine Alternative Collection: 10mL urine

TAT: 24 hours Test Schedule: Daily Amphetamine Screen Method: Immunoassay Urine Test Facility: AWL

Included in drug screen panels Drug Screen Abbreviated, Drug Screen Basic or Drug Screen Comprehensive.

Processing: Refrigerate if held overnight.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: Next Day Amylase Isoenzymes Test Schedule: Monday-Friday Morning Method: Enzymatic Calculation Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Specimen Stability: Room Temperature: No

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Refrigerated: 14 days Frozen: No Test Code: CHANTILLY TC 845

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Transport on ice.

Preferred Collection/Volume: GRN Li Hep Volume: 0.5mL p Alternative Collection: RED SST Volume: 0.2mL s

TAT: 24 hours Amylase Level Test Schedule: Daily Method: Colorimetric Rate Reaction Test Facility: SMC, INT, BVT, AWL, CPK, LVK

Processing: Centrifuge. Refrigerate if held overnight.

Preferred Collection/Volume: Green Lithium/4.0 mL body fluid Alternative Collection: Red Top 10/0.5 mL body fluid

TAT: One hour STAT - 24 Hours for Routine Test Schedule: Daily Amylase Level Body Method: Colorimetric Rate Reaction Fluid Test Facility: AWL, SMC

Use separate LIS requests if both blood and body fluids are ordered.

Processing: Refrigerate if held.

Preferred Collection/Volume: AP99 with 12x75 Aliquot/4.0mL Alternative Collection: 1.0mL

TAT: 24 hours Test Schedule: Daily Amylase Level Urine Method: Colorimetric Rate Reaction Test Facility: AWL

Processing: Aliquot to 12x75 tube. Refrigerate

Preferred Collection/Volume: Timed urine (no preservative) 12x75 Aliquot/4.0mL Alternative Collection: 1.0mL

TAT: One hour STAT - 24 Hours for Routine Test Schedule: Daily Method: Colorimetric Rate Reaction Amylase Timed Urine Test Facility: AWL

Specimen must be refrigerated. Preservatives are not acceptable. See Amylase level random urine panel for random specimen.

Processing: Record collection interval hrs. Measure and record total vol.

Preferred Collection/Volume: REDSST Volume: 1.0mL s

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Alternate Collection: RED10

TAT: 1-3 days Test Schedule: Monday through Friday Test Method: Multiplex Flow Immunoassay Testing Location: Airport Way Regional Laboratory

Specimen Stability: ANA Screen Refrigerated: 7 days Frozen: 30 days Specimen Rejection: Gross hemolysis Gross lipemia

Processing: Centrifuge. Aliquot if collected in RED10 Tranport: To AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: Prefrerred Specimen: Random Urine AP99 Volume 50.0 mL urine

TAT: Report available: 10 Days Test Schedule: Monday-Friday Morning Method: Gas Chromatography Mass Spectrometry Test Facility: University of Virginia Box 800168 Anabolic Steroids, Charlottesville, VA 22905 Qualitative, Urine Specimen Stability Room Temperature: unacceptable Refrigerated: 2 weeks Frozen: not estab Test Code: CHANTILLY TC 28017 to: University of VA. U Va test name = STEROIDS.

Transport: Transport on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Reports in 4 days. Test Schedule: Sets up 6 days a week. Method: Liquid Chromatography Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130 Androstenedione Clinical Data: Androstenedione may be useful in evaluating patients with androgen excess and managing patients with congenital adrenal hyperplasia (CAH).

Patient Preparation: Early morning specimen is preferred.

Specimen Stability: Serum or Plasma Room temperature: 14 Days Refrigerated: 28 Days Frozen: 2 Years Test Code: SJC TC 17182

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Note if serum or plasma on

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specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL Serum Alternative Collection: RED SST Volume: 1.0 mL serum

TAT: 1 day (not reported on Sunday) Test Schedule: Monday through Saturday; Continuously Method: Spectrophotometry Test Facility: Mayo Medical Laboratories Angiotensin Converting 3050 Superior Drive NW Enzyme Rochester, MN 55901

Specimen Stability Refrigerated: 7 days Frozen: 180 days Test Code: MAYO TC 8285

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Freeze sample if held. Transport frozen on ice

Collection Instructions Preferred Collection/Volume: CSF Volume: .5 mL

TAT: 1-5 days Test Schedule: Test Method: Kinetic Test Facility: Focus Diagnostics via Mayo Laboratories Focus Diagnostics, Inc. 5785 Corporate Avenue Cypress, California 90630

Angiotensin Converting Client Services: (800) 445-4032 Enzyme CSF

CSF levels of angiotensin converting enzyme (ACE) are increased in approximately 50% of patients with neurosarcoidosis, but in less than 10% of systemic sarcoidosis patients without neurologic manifestations.

Transport 2-8 centigrade TC: 64005

Transportation: Transport to AWL on ice

Preferred Collection/Volume: RED 10 Volume: 1.0mL s

TAT: 4 days Test Schedule: Tuesday, Thursday, and Saturday AM Test Method: Chemiluminescence (CL) Test Facility: Quest Diagnostics Nichols Institute Valencia

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27027 Tourney Road Valencia, CA 91355-5386

Clinical Significance AMH/MIS may be used in the investigation of ovarian reserve and the perimenopausal transition in women; the detection and onset of puberty in the young, the differential diagnosis of intersex disorders; the diagnosis of cryptorchidism and anorchidism, and the evaluation of male gonadal function in all ages.

Referred Tests: Transport Temperature Anti Mullerian Frozen Hormone Specimen Stability: Room temperature: Unacceptable Refrigerated: 5 days Frozen: 30 days Reject Criteria Gross hemolysis • Icteric • Lipemia TC# 16842X

Processing : Centrifuge within one hour of collection and aliquot into Referred Tests aliquot tube. Freeze. Transport: Frozen on ice.

Preferred Collection/Volume: x2 Pink EDTA Volume: 12 mL wb (EDTA) AND x2 RED 10 Volume: 20 mL wb Antibody Identification, ARC For collection of specimens to be sent to American Red Cross Red Cell Reference Lab. Transport specimens to KSMC Blood Bank. KSMC Blood Bank staff responsible to complete necessary requisition and arrange transport to ARC. Contact KSMC Blood Bank for information or questions.

Preferred Collection/Volume: Pink EDTA Volume: 5.0 mL Alternative Collection: LAV EDTA RED 10 (do not centrifuge) Antibody Screen Absolute Minimum Volume: 1.0 mL Prenatal The Blood and Blood Component form and identifier signature are NOT required for this test.

Preferred Collection/Volume: Pink EDTA Volume: 5.0 mL Alternative Collection: LAV EDTA RED 10 (do not centrifuge) Antibody Screen. Absolute Minimum Volume: 1.0 mL

Use this test code for routine antibody screen orders only, not for transfusion testing.

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Preferred Collection/Volume: Pink EDTA Volume: 5.0 mL Antibody Titer, Red Alternative Collection: LAV EDTA Cell, Prenatal RED 10 (do not centrifuge) Absolute Minimum Volume: 1.0 mL

Preferred Collection/Volume: RED SST Volume: 1.0 mL s

TAT: Next Day Test Schedule: Monday-Friday Method: Immunoassay Test Facility: Airport Way Regional Laboratory Antiglomerular Base Sam/Tox Mem Ab Specimen Stability: Refrigerated: 7 days Frozen: 30 days

Processing: Centrifuge Transport: To AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: Red Top Tube/2.5 mL serum Alternative Collection: Red serum separator/0.2mL

TAT: 24-48 hours Antimitochondrial Test Schedule: M-F Antibody Screen Method: Immunofluorescence Test Facility: AWL

Processing: Cent, Aliquot, refrigerate if held.

Collection Instructions Preferred Collection/Volume: RED SST Volume: 1.0 mL

Antineutrophil Specimen Handling Cytoplasmic Antibody Processing: Centrifuge. Refrigerate if held. Transport: To AWL in blue tote.

Panel includes Myeloperoxidase Antibody and Proteinase-3 AntibodyAlso known as ANCA or Vasculitis Panel.

Preferred Collection/Volume: RED 10 Volume: 2.5 mL s Alternative Collection: RED SST also acceptable

TAT: 24-48 hours Antinuclear Antibody Test Schedule: M-F Screen Method: Immunofluorescence Test Facility: AWL

Positive homogenous or rim pattern will reflex DNA Antibody panel (DNA).

Processing: Centrifuge, aliquot, and refrigerate if held

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Preferred Collection: 2 BLUE CIT Tube must be filled to minimum fill indicator Volume: 1mL plasma Neonates minimum volume plasma 100 µl, if not combined with other tests.

TAT: 2-7 days Test Schedule: Once per week Method: Chromogenic substrate Test Facility: AWL

Patient Preparation: If using a butterfly, a citrate discard tube must be used first, Antithrombin III when citrate is the first tube collected. Activity Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Centrifuge immediately and aliquot 1.0 mL plasma into 2 aliquot tubes using a plastic pipette. Do Not Use Filter. Avoid buffy coat. Prepare platelet poor plasma onsite. Freeze solid NOTE: Aliquots may be shared with Protein C and Protein S if ordered If needed STAT, contact testing department (AWL-SAM) to make arrangements for same day testing. Specimen must arrive by noon. Transport: Transport frozen on ice. DO NOT ALLOW TO THAW

Preferred Collection/Volume: RED 10 or RED SST Volume: 1.0 mL serum

TAT: 3 Days Test Schedule: Monday-Friday Morning Method: Indirect Immunofluorescence Assay Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave, Suite 200 Cypress, CA 90630

Clinical Data: Assay is for IgG antibodies to St. Louis, western equine, and eastern equine, and the California encephalitis group Arbovirus IgM, Mac EIA Specimen Stability: Room Temp: 4 days Refrigerated: 7 days Frozen: 30 days Frozen -70 degrees C: Indefinitely Allow blood samples to clot at room temperature. Centrifuge serum specimens within 1 hour of collection; transfer serum to sterile, plastic, screw- capped vial(s). Test Code: Quest TC 13522

Processing: Centrifuge specimen within 1 hour of collection; aliqout into Referred Tests aliqout tube. Transport: Refrigerate if held

Preferred Collection/Volume: Red 5mL

TAT: One week Arginine Stimulation, Test Schedule: Friday 105 Min Method: Immunoassay Test Facility: AWL

This panel is only ordered by Beaverton Pediatric Endocrinology. See WI 1-30-322,

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Insulin Arginine Stimulation Test, prior to the start of the test for complete collection and testing instructions.

Processing: Red 5:Centrifuge, aliquot, freeze. Transport: Transport frozen on ice for Growth Hormone testing

Preferred Collection/Volume: Red 5mL

TAT: One week Test Schedule: Friday Method: Immunoassay Arginine Stimulation, Test Facility: AWL 120 Min This panel is only ordered by Beaverton Pediatric Endocrinology. See WI 1-30-322, Insulin Arginine Stimulation Test, prior to the start of the test for complete collection and testing instructions.

Processing: Red 5:Centrifuge, aliquot, freeze. Transport: Transport frozen on ice for Growth Hormone testing

Preferred Collection/Volume: Red 5mL

TAT: One week Test Schedule: Friday Method: Immunoassay Arginine Stimulation, Test Facility: AWL 135 Min This panel is only ordered by Beaverton Pediatric Endocrinology. See WI 1-30-322, Insulin Arginine Stimulation Test, prior to the start of the test for complete collection and testing instructions.

Processing: Red 5:Centrifuge, aliquot, freeze. Transport: Transport frozen on ice for Growth Hormone testing

Preferred Collection/Volume: Green Lithium Heparin Separator and Red 5mL

TAT: One week Test Schedule: Friday Method: Immunoassay Test Facility: AWL Arginine Stimulation, 15 Min This panel is only ordered by Beaverton Pediatric Endocrinology. See WI 1-30-322, Insulin Arginine Stimulation Test, prior to the start of the test for complete collection and testing instructions.

Processing: Red 5:Centrifuge, aliquot, freeze. Transport: Transport frozen on ice for Growth Hormone testing

Preferred Collection/Volume: Red 5mL

TAT: One Week Test Schedule: Friday Arginine Stimulation, Method: Immunoassay 150 Min Test Facility: AWL

This panel is only ordered by Beaverton Pediatric Endocrinology. See WI 1-30-322, Insulin Arginine Stimulation Test, prior to the start of the test for complete collection and testing instructions. Whole blood for Glucose, performed on site.

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Processing: Red 5:Centrifuge, aliquot, freeze. Transport: Transport frozen on ice for Growth Hormone testing

Preferred Collection/Volume: Green Lithium Heparin Separator and Red 5mL

TAT: One Week Test Schedule: Friday Method: Immunoassay Test Facility: AWL Arginine Stimulation, This panel is only ordered by Beaverton Pediatric Endocrinology. See WI 1-30-322, 30 Min Insulin Arginine Stimulation Test, prior to the start of the test for complete collection and testing instructions. Whole blood for Glucose, performed on site.

Processing: Red 5:Centrifuge, aliquot, freeze. Transport: Transport frozen on ice for Growth Hormone testing

Preferred Collection/Volume: Green Lithium Heparin Separator and Red 5mL

TAT: One Week Test Schedule: Friday Method: Immunoassay Test Facility: AWL Arginine Stimulation, This panel is only ordered by Beaverton Pediatric Endocrinology. See WI 1-30-322, 45 Min Insulin Arginine Stimulation Test, prior to the start of the test for complete collection and testing instructions. Whole blood for Glucose, performed on site.

Processing: Red 5:Centrifuge, aliquot, freeze. Transport: Transport frozen on ice for Growth Hormone testing

Preferred Collection/Volume: Green Lithium Heparin Separator, Red 5, and Red Serum Separator

TAT: One Week Test Schedule: Friday Method: Immunoassay Test Facility: AWL

This panel is only ordered by Beaverton Pediatric This panel is only ordered by Arginine Stimulation, Beaverton Pediatric Endocrinology. See WI 1-30-322, Insulin Arginine Stimulation 60 Min Test, prior to the start of the test for complete collection and testing instructions. Whole blood for Glucose, performed on site.-30-322, Insulin Arginine Stimulation Test, prior to the start of the test for complete collection and testing instructions. Whole blood for Glucose, performed on site.

Processing: Red 5: Centrifuge, aliquot, freeze. Red Serum Separator:Centrifuge, aliquot, refrigerate. Transport: Send on ice for Cortisol testing, send frozen on ice for Growth Hormone

Preferred Collection/Volume: Red 5mL

TAT: One Week Test Schedule: Friday

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Method: Immunoassay Test Facility: AWL

This panel is only ordered by Beaverton Pediatric Endocrinology. See WI 1-30-322, Arginine Stimulation, Insulin Arginine Stimulation Test, prior to the start of the test for complete collection 75 Min and testing instructions.

Processing: Red 5:Centrifuge, aliquot, freeze. Transport: Transport frozen on ice for Growth Hormone testing

Preferred Collection/Volume: Red 5mL

TAT: One Week Test Schedule: Friday Method: Immunoassay Test Facility: AWL Arginine Stimulation, 90 Min This panel is only ordered by Beaverton Pediatric Endocrinology. See WI 1-30-322, Insulin Arginine Stimulation Test, prior to the start of the test for complete collection and testing instructions.

Processing: Red 5:Centrifuge, aliquot, freeze. Transport: Transport frozen on ice for Growth Hormone testing

Preferred Collection/Volume: Green Lithium Heparin Separator, Red 5, and Red Serum Separator

TAT: One Week Test Schedule: Friday Method: Immunoassay Test Facility: AWL Arginine Stimulation, Baseline This panel is only ordered by Beaverton Pediatric Endocrinology. See WI 1-30-322, Insulin Arginine Stimulation Test, prior to the start of the test for complete collection and testing instructions.

Processing: Red 5: Centrifuge, aliquot, freeze. Red Serum Separator:Centrifuge, aliquot, refrigerate. Transport: Send on ice for Cortisol testing, send frozen on ice for Growth Hormone

Preferred Collection/Volume: Collect in Acid Washed Metal Free Container Preferred Specimen: 24 Urine Volume: 7.0 mL urine

TAT: Report available: Next Day Test Schedule: Monday, Wednesday, Friday Afternoon Method: Inductively Coupled Plasma-Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Arsenic 24 Hour Urine Chantilly, VA 20153

Specimen Stability: Room Temperature: 2 days Refrigerated: 5 days Frozen: 2 weeks Test Code: CHANTILLY TC 14430

Processing: Mix sample well and Aliquot urine into acid washed urine aliquot. Note on sample total volume and preservative used during collection.

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Transport: Refrigerate if held

Preferred Collection/Volume: Trace Metal Free DarkBlue EDTA Volume: 7.0 mL wb Alternative Collection: Trace Metal Free Dark Blue Green NA Hep Absolute Minimum Volume : 2mL wb

TAT: Report available: 3 Days Test Schedule: Monday, Wednesday, Friday Afternoon Method: Inductively Coupled Plasma-Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Limitations Arsenic may only be present in blood for 4-6 hours. This test is most accurate in acute poisoning. Clinical Significance Arsenic Level This assay is used to monitor exposure to arsenic, wellness, and therapy during treatment of chronic myelocytic leukemia.

Patient Preparation: Patient should refrain from eating seafood and taking herbal supplements for at least 3 days before specimen collection.

Specimen Stability: Room Temperature: 10 Days Refrigerated : 10 Days Frozen : 1 month TC# 269 Rejection Criterion: Clotted specimens

Processing: Do not centrifuge. Transport: Refrigerate if held

Preferred Collection/Volume: Random Urine collect urine in acid washed container. Volume:7.0 mL urine

TAT: Report available: 3 Days Test Schedule: Monday, Wednesday, Friday Afternoon Method: Inductively Coupled Plasma-Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Arsenic Level Urine Patient Preparation: Patient should refrain from eating shellfish, shrimp, crab, lobster, and bottom-feeders, such as flounder, at least 3 days before specimen collection.

Specimen Stability: Room Temperature: 2 days Refrigerated: 5 days Frozen: 2 weeks Test Code: CHANTILLY TC 270X

Processing: Submitted sample must be collected in acid washed contaienr.. Transport: Refrigerated

Preferred Collection/Volume: Insect (tick/lice) placed into sterile screwcapped container. Add 70% alcohol to cover insect. Alternative Collection: Hair or skin scrapings (for lice) placed into sterile screw

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topped container.

TAT: 1- 2 Days Test Schedule: Daily Method: Microscopy Test Facility: AWL

Clinical Data: Specimen: Adult forms or larvae eggs of insects. Most common specimens are Arthropod ticks, maggots, or lice. Identification Patient History: n/a

Submit sample in sterile screw topped container.

Rejection criteria: Dehydrated insect

Processing: Room Temperature Transport: Room temperature

Preferred Collection/Volume: Collection Instructions: Draw Mon-Thursday only Yellow ACD B or punch biopsy placed in special medium container available from AWL Volume: Whole Blood Yellow ACD B__7.0 mL or tissue __4mm Alternative Collection: Yellow ACD A Volume 7.0 mL Whole Blood

TAT: Blood: 8 days Tissue: 30-45 days Test Schedule: Specimens are processed Monday through Sunday. Assay is performed Thursday; 8 a.m. Method: Colorimetric Enzyme Assay Arylsulfatase A, Blood Test Facility: Mayo Medical Laboratories or Tissue 3050 Superior Drive NW Rochester, MN 55901

Clinical Data: Blood specimens must arrive within 48 hours of collection to be stabilized. Collect blood specimen Monday-Thursday only. Specimen must reach AWL before 1300.

Specimen Stability: Room Temperature 48 hours Refrigerated 48 hours. Specimen should be drawn and packaged as close to shipping time as possible. Test Code: MAYO TC ACD 8779 and BX 8778

Processing: Specimen must arrive within 48 hours of draw to be stabilized. Draw specimen Monday through Thursday only.

Preferred Collection/Volume: Red Serum Separator/1.0mL serum Alternative Collection: Red Top Tube/0.5mL serum

TAT: 24 hours Test Schedule: Daily ASO Screen Method: Turbidometry Test Facility: AWL

Processing: Centrifuge. Aliquot if Red Top Tube. Refrigerate if held.

Preferred Collection/Volume: RED 10

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Volume: 4 mL Serum

TAT: Performed once per week. Test Facility: OHSU Laboratories Occupational and Environmental Allergy Lab 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Clinical Data: Precipitin panels demonstrate the presence of precipitating antibody (predominantly IgG and IgM) by immunodiffusion (Ouchterlony) in gel medium. This panel contains Aspergillus Allergy several of the most pathogenic Aspergillus species from different sources. Most clinically significant results occur when multiple and sharp precipitin lines are found. A trace precipitin band Panel is rarely important and a weak band is equivocal.

Send to OHSU ASAP Specimen Stability: Room Temperature: 24 hours Refrigerated: 7 days Test Code: OHSU TC 064-0901

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection: RED 10 (No Gel Barrier) Volume: 1mL Serum Minimum Volume: .05 mL Serum

TAT: 3-5 Days Testing Location: ARUP Laboratories 500 Chipeta Way Aspergillus Antibody Salt Lake City, UT 84108-1221

Specimen Stability: Room Temperature: 48 hours Refrigerated: 14 day Frozen (-20 °C): 12 months TC# 97771

Processing: Centrifuge within 2 hours of collection and aliquot into a Referred Test aliquot tube Transportation: Refrigerate if held

Preferred Collection/Volume: REDSS5 Volume: 2mL s Alternative Collections: Bronchial lavage/wash submitted in a sterile, unopened plastic leakproof container Volume: 2mL Frozen

TAT: Next Day Test Schedule: Monday-Friday evening shifts Test Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive

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Chantilly, VA 20153

Clinical Significance: The Aspergillus EIA is used for the detection of galactomannan antigen in serum. The Aspergillus EIA is an aid in the early diagnosis of invasive aspergillosis. This assay is to be used and test results interpreted in conjunction with other conventional diagnostic procedures such as microbiological culture, histologic examination of biopsy samples and other signs and symptoms for detection of Aspergillus infection. Alternative Names(s) Galactomannan Antigen Aspergillus Antigen Unopened serum or Bronchial Alveolar Lavage (BAL) may be stored at 2-8°C for up to 5 days before testing, or for only 48 hours after they have been opened. (Do not reject if received refrigerated.) Longer storage requires freezing -70ºC . Care is required to avoid contamination of specimens with mold during collection, processing, and shipment. Note: This assay has not been evaluated for use with plasma, urine, or CSF. TC#14950

Processing: Centrifuge and freeze Transport: Frozen on ice.

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p Alternative Collection: RED SST Volume: 0.2mL s

TAT: One hour STAT - 24 Hours for Routine AST Test Schedule: Daily Method: Photometric, L-Asparate with Alpha-Oxoglutarate Test Facility: AWL, SMC, INT, BVN, CPK. LVK

Processing: Centrifuge. Refrigerate if held.

Preferred Collection: LAV EDTA Alternative Collection: LAV EDTA Microtainer Minimum Volume: 1 mL

TAT: 24 hours for routine specimens Automated Blood Count Testing Method: 5-Part diff Sysmex Hematology cell counter Stability:

{ Specimen is stable for 24 hrs at room temperature for all parameters.

{ Refrigerated specimens are stable for 72 hours for hemogram (ABC) parameters.

{ Specimens 48-72 hrs old require evaluation of all abnormal differentials.

Rejection Criteria:

{ Clotted specimens

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{ Hemolyzed specimens

{ IV contaminated specimens

Note: Test on site whenever possible if specimen is collected in a microtainer.

Processing: Handle microtainers ASAP and test on-site when possible. Transport: Transport specimens to Airport Way Laboratory.

Preferred Collection/Volume: Sterile Urine Cup/50 mL urine Alternative Collection: 10 mL urine

TAT: 24 hours Test Schedule: Daily Method: Immunoassay Barbiturate Screen Test Facility: AWL Urine Included in drug screen panels Drug screen basic urine and Drug Screen Comprehensive.

Processing: Refrigerate if held overnight.

Preferred Collection/Volume: Sterile Urine Cup/50 mL urine Alternative Collection: 10 mL urine

TAT: 3 days Test Schedule: T-Th-Sat Barbiturates Method: GCMS Confirmatory Test Facility: AWL

To order a drug screen see panels Barbituate Screen Urine, Drug Screen Basic, Comprehensive Urine

Processing: Refrigerate if held overnight.

Preferred Collection: Red 10 Volume: 1.0 mL Serum

TAT: Report is available next day Test Schedule: Set up is Tuesday and Thursday morning Method: Immunofluorescence Assay (IFA) Test Facility: Bartonella henselae Quest Diagnostics Nichols Institute Antibody IgG & IgM 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Bartonella. henselae infections have been associated with many clinical presentations, including cat scratch disease, bacillary angiomatosis, peliosis hepatis, and bacteremia. B. quintana infections have been associated with trench fever and bacillary angiomatosis.

Specimen Stability: Room temperature: 4 days Refrigerated: 5 days

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Frozen: 30 days Test Code: TC# 34251X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: GRN Li Hep PST Volume: 2.0 mL p Alternative Collection: RED SST Volume: 2.0 mL s

TAT: 1 HR / 24 HR Test Schedule: Daily Basic Metabolic Panel Method: See Individual Assays Test Facility: SMC, AWL, INT, BVN, CPK, LVK

Analytes include Na,K, Cl, CO2, Non-fasting GLU, BUN,Creat and Calcium.

Processing: Centrifuge. DO NOT REMOVE CAP. Refrigerate if held.

Preferred Collection/Volume: GNS5/2.0 mL p Alternative Collection: SSRED5/1.0 mL p

TAT: 1 HR / 24 HR Basic Metabolic Screen, Test Schedule: Daily Fasting Method: See Individual Assays Test Facility: SMC, AWL, INT, BVT, CAS, LVK

Processing: Centrifuge. DO NOT REMOVE CAP. Refrigerate.

Preferred Collection: Whole Blood or Bone Marrow in LAV EDTA Volume: 5 -10 mL Whole blood 0.5 -1 mL Bone Marrow Note: Sample type, collection date and time must be written clearly on the specimen container.

BCR/ABL1 Gene TAT: 7-10 days Translocation Testing Schedule: 1x weekly Quantitative Testing Location: Molecular Department Airport Way Regional Laboratory 13705 Ne Airport Way Portland, OR 97230 Specimen Stability: Refrigerated: 48 hours Room remperature: 24 hours Rejection Criteria: Unlabeled specimen, heparin contaminated or gross hemolysis

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specimens are not accepted.

Note: Specimens can be collected Monday -Friday, but MUST BE RECEIVED AT AWL NO LATER THAN 1:00PM ON FRIDAY FOR TIMELY PROCESSING. Please call Client Services at 503.258.6900 (tie line 35x6900) for questions about Friday collections.

Clinical Data: Quantitative RT-PCR for t(9;22) BCR-ABL1 RNA relative to ABL1 housekeeping gene. Procedure Components: BCQ BCR/ABL1 Gene Trans BCR/ABL1 Gene Rearrangement, Quantitative PCR

Processing: Do not centrifuge or freeze Whole Blood or Bone Marrow. Transport: Ship specimen immediatley at room temperature or refrigerated.

Preferred Collection/Volume: AP99 Random Urine/50 mL u Alternative Collection: 10 mL u

TAT: 1 HR / 24 HR Test Schedule: Daily Benzodiazepine Screen Method: Immunoassay Urine Test Facility: AWL

Included in drug screen panels Basic and Comprehensive

Processing: Refrigerate if held overnight.

Preferred Collection/Volume: Random Urine in AP99 Volume: 20.0 mL Urine

TAT: Report available: 4 Days Test Schedule: Monday-Friday Afternoon Method: Gas Chromatography Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Benzodiazepine, Urine, Chantilly, VA 20153 GC/MS Specimen Stability: Room Temperature: 3 days Refrigerated: 2 weeks Frozen: 1 year Test Code: CHANTILLY TC 19137

Transport: Refrigerate if held.

Preferred Collection/Volume: Random Urine in Acid Washed container Volume: 3.0 mL Urine

TAT: 3 Days Test Schedule: Thusday AM Method: Colorimetry, Inductively Coupled Plasma/Mass Spectrometry Test Facility: NMS LABS 3701 Welsh Road Willow Grove, PA 19090

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Patient Preparation: Patient should avoid exposure to gadolinium-based contrast media for 48 hours prior to cample collection

Specimen Stability: Room Temperature: 7 day(s) Beryllium, Urine Refrigerated: 7 day(s) Frozen (-20 °C): 14 day(s) Test Code: CHANTILLY TC 13777 TO NMS TC# 0638U

Transport: Refrigerate if held

Preferred Collection/Volume: Avoid hemolysis RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Saturday Afternoon Method: Nephelometric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Beta 2 Microglobulin Patient Preparation: Fasting specimens are preferred.

Specimen Stability: Room Temperature: 5 Days Refrigerated: 7 Days Frozen: 30 Days Test Code: CHANTILLY TC 85289

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: CSF in Sterile Container, leak-proof Volume: 1.0 mL CSF

TAT: Report available: Next Day Test Schedule: Monday-Saturday Morning Method: Nephelometric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Beta 2 Microglobulin Clinical Data: Increased levels of Beta-2-Microglobulin in CSF may be CSF found in infectious and cerebrovascular disorders.

Specimen Stability: Room Temperature: 7 days Refrigerated: 28 days Frozen: 28 days (-20c) Test Code: CHANTILLY TC 5500X

Processing: Freeze soild Transport: Transport frozen on ice

L

TAT: Stat within 1 HR, Routines within 1 day. Testing Schedule: Daily

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Method: Immuno-assay (rapid screen) Testing Location: Medical Offices and Medical Centers Specimen Stability: Room Temperature: 24 hours Beta hCG Qualitative Refrigerated: 48 hours Urine First morning specimen is preferred as it contains a higher concentration of the pregnancy hormone.

Preferred Collection/Volume: GRN Li Hep PST Volume: 1.0mL p Alternative Collection: SSRED GRN Li Hep GRN Na Hep RED10 Absolute Minimum Volume: 0.5mL

TAT: 1 HR STAT / 24 HR ROUTINE Test Schedule: Daily Method: Chemiluminescent Immunoassay Test Facility: Airport Way Regional Laboratory Beta hCG Quantitative Sunnyside Medical Center Westside Medical Center

Used for evaluating and/or following possible ectopic pregnancy, threatened abortion, trophoblastic disease or as a tumor marker.

See panel Beta hCG Tumor CSF for CSF specimens.

Processing: Centrifuge Aliquot if serum Refrigerate if held Transportation: Transport to testing location

Preferred Collection/Volume: CSF Sterile container/0.5 mL Alternative Collection: 0.3 mL

TAT: 1 HR / 24 HR Test Schedule: Daily Beta HCG Tumor CSF Method: Immunoassay Test Facility: SMC, AWL, INT

Preferred tube number for CSF collections is #1 but any tube may be used.

Processing: Refrigerate if held overnight.

Preferred Collection: Grn Li Hep PS Volume: 0.5 mL Alternative Collection: Red SS Beta Hydroxybutyrate Absolute Minimum Volume: 0.2mL

TAT: STAT specimens resulted with in 1 hour Routine specimens resulted with in 24 Hours after arrival in lab Testing Schedule: Daily

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Method: Colorimetric Testing Location: Sunnyside Medical Center and Westside Medical Center

Processing: Centrifuge and refrigerate

Preferred Collection/Volume: Body Fluid in Sterile leak proof container Volume: 0.5 mL Body Fluid Alternative Collection: Nasal, otic, and other Non-CSF fluids are acceptable. Volume 0.5 mL NOTE: CSF is NOT acceptable.

TAT: Report available: Next Day Test Schedule: Monday-Friday Evening Method: Electrophoresis Immunofixation Electrophoresis Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Beta-2 Transferrin, Chantilly, VA 20153 Body Fluid Clinical Data: The presence of Beta-2 Transferrrin in nasal or ear fluid or in wound drainage, following head trauma, surgery, or from tumors or congenital malformation, clearly indicates CSF leaking into these passages or fluids creating a pathway for life-threatening, central nervous system infection.

Specimen Stability: Room Temperature: 30 Days Refrigerated: 30 Days Frozen: 30 days Test Code: CHANTILLY TC 10640

Tranpsort: Refrigerate if held

Preferred Collection/Volume: RED 10 Volume: 0.5 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning Method: Enzymatic Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Bile Acids Total Patient Preparation: Patient should fast for 8 hours prior to collection. Specimen Stability: Room Temperature: Unacceptable Refrigerated: 7 days Frozen: Not Established Test Codes: CHANTILLY TC 7802

Processing: Centrifuge and aliquot serum into Referred Tests aliquot tubes within 1 hour of collection. Transport: Transport on ice.

Preferred Collection/Volume: PROTECT FROM LIGHT Preferred Specimen: Amniotic Fluid (Amber Container) Volume: 3.0-5.0 mL Amniotic Fluid

TAT: 1-2 days Test Schedule: 7 days/week Method: Spectrophotometry (Delta OD 450 nm)

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Test Facility: Legacy Central Laboratory 1225 NE 2nd Ave Portland, OR 97232

Specimen Stability: Protect specimen from light Room Temperature: 2 days Refrigerated: 1 week Bilirubin Amniotic Frozen: 1 year Fluid LEGACY CS: 503-413-1234. NO TEST CODE

Processing: PROCESS STAT and PROTECT FROM LIGHT. Cab directly to Legacy Central Lab with completed Legacy Requisition and Specimen Transport Form 23-09-204. Gestational Age and pertinent clinical data must be submitted with requisition. Avoid contamination with blood. Transport: Transport on ice and protect from light

Preferred Collection/Volume: LIGREEN4 (Green Li)/4.0mL bf Alternative Collection: RD10 (Red Top Tube)/0.2mL bf

TAT: ROUTINE Test Schedule: Daily Bilirubin Body Fluid Method: Photometric, Diazonium Salt (DPD) Test Facility: SMC, AWL

Use separate LIS requests if both blood and body fluids are ordered.

Processing: Refrigerate.

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5 mL p Alternative Collection: RED SST or serum acceptable Volume: 0.2 mL s

TAT: 1 HR / 24 HR Test Schedule: Daily Method: Photometric, Diazonium Salt (DPD) in acid Bilirubin Direct Test Facility: SMC, INT, BVN, CPK, LVK

For patients over 21 days old. See panel Direct Bilirubin Baby for neonates and infants. Direct Bilirubin will be assayed as a reflex test if Total Bilirubin is greater than 1.1.

Processing: Centrifuge, refrigerate

Preferred Collection: GRN Micro Li Hep w/sep Alternative Collection: Amber Micro w/sep Volume: 0.5mL plasma (Absolute Minimum Volume: 0.1 mL plasma)

TAT: STAT 1 Hour Routine 24 Hours Bilirubin Direct Test Schedule: Daily Neonatal Method: Photometric, Diazonium Salt (DPD) in acid Test Facility: BVN, CPK, INT, LVK, NLR, SKY, SMC, WMC

Intended for neonates and infants, generally used up to 30 days of age but continue to order if bilirubin is monitored beyond 30 days of age. Will combine with TBB (T.Bilirubin Neonatal) if ordered together. Note: TEST NOT PERFORMED AT AWL

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Processing: Centrifuge. Protect specimens collected in GRN Li Hep from light. Refrigerate.

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5 mL p Alternative Collection: RED SST or serum acceptable Volume: 0.5 mL s

TAT: 1 HR / 24 HR Test Schedule: Daily Method: Photometric, Diazonium Salt (DPD) Test Facility: Airport Way Regional Laboratory SunnySide Medical Center Bilirubin Total Interstate Medical Office Cascade Medical Office Beaverton Medical Office Longview Kelso Medical Office

See panel Bilirubin Total Neonates for neonatal and infant testing. If Total Bilirubin is greater than 1.1, Direct Bilirubin test will be reflexed

Processing: Centrifuge. Refrigerate if held.

Preferred Collection: GRN Micro Li Hep w/sep Alternative Collection: Amber Micro w/sep Volume: 0.5 mL plasma (Absolute Minimum Volume: 0.1 mL plasma)

TAT: STAT 1 Hour Routine 24 Hours Test Schedule: Daily Method: Photometric, Diazonium Salt (DPD) Bilirubin Total Neonatal Test Facility: BVN, CPK, INT, LVK, NLR, SKY, SMC, WMC

Intended for neonates and infants. Generally used up to 30 or age days but continue to order if bilirubin is monitored beyond 30 days of age. Note: TEST NOT PERFORMED AT AWL

Processing: Centrifuge. Protect specimens collected in GRN Li Hep from light. Refrigerate.

Preferred Collection: RED 10 Alternative: LAV EDTA, GREEN NA HEP, GREEN LI HEP Volume: 3.0 mL Minimum Volume: Biotinidase 1.0 mL TAT: Report available within 4 days Test Schedule: Tuesday and Thursday Mornings Testing Location: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130 TC# 70132X

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Specimen Stability: Room Temperature: unacceptable Refrigerated: unacceptable Frozen: 30 days

Processing: Centrifuge specimen within 1 hour of collection, aliquot into Referred Test aliquot tube, and freeze. Transport: Send specimen frozen on ice Note: Whole blood is not an acceptable specimen type.

Preferred Collection/Volume: RED 10 Volume: 10 mL Serum

Test Schedule: Performed once per week Test Facility: OHSU Laboratories Dillehunt Hall room 3020 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Clinical Data: Precipitin panels demonstrate the presence of precipitating antibody Bird Fancier Precipitin (predominantly IgG and IgM) by immunodiffusion (Ouchterlony) in gel medium. This tests for Panel antibody to a variety of birds (both pet and commericial) as well as an assortment of bird components. Most clinically significant results occur when multiple and sharp precipitin lines are found. A trace precipitin band is rarely important and a weak band is equivocal.

Specimen Stability: Refrigerated only Test Code: OHSU TC 064-0905

Processing: Centrifuge and aliquot. Transport: Transport on ice.

Preferred Collection/Volume: RED 10 Volume: 10 mL Serum

Test Schedule: Performed once per week Test Facility: OHSU Laboratories Dillehunt Hall room 3020 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Bird Fancier Precipitins Clinical Data: This tests for antibody to a variety of birds (both pet and commercial) as well as an assortment of bird components. Included are total serum IgE, the bird fancier precipitin panel of w IgE Panel twelve antigens along with allergen-specific-IgE to Parrot feathers, droppings, serum and pigeon droppings plus chicken feathers.

Specimen Stability: Refrigerated only Test Code: OHSU TC 064-0943

Processing: Centrifuge and aliquot Transport: Transport on ice.

Preferred Collection/Volume: Trace metal free Dark Blue EDTA Volume: 4.0 mL whole blood

TAT: Report available: 3-4 days Test Schedule: Set up: 5 days a week Method: Inductively Coupled Plasma/Mass Spectrometry (ICP/MS) Test Facility: Quest Diagnostics Nichols Institute

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33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Excessive use of Bismuth containing medications may cause renal damage and other adverse effects.

Specimen Stability: Bismuth Room temperature: 48 hours Refrigerated: 7 days Frozen: Unacceptable Test Code: SJC TC 5374X

Processing: Do not centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume : LAV EDT A Volume: 1.0mL p Alternative Collection: SSRED

TAT: 4 days Test Schedule: Tuesdays and Thursday mornings Test Method: Real-Time Polymerase Chain Reaction (RT-PCR) Testing Location: Oregon Health Sciences University Portland, Oregon

Clinical Significance BK Virus DNA, Quant This test is used to determine the presence of BK Virus DNA in patient's specimens. RT PCR Plasma Detection of the virus in these specimens may be indicative of an active infection, as PCR detects the presence of the virus, and not the host's reaction to the virus Polyomavirus BK DNA detection in urine or plasma is associated with an increased risk of graft rejection in renal recipients. Quantitative testing may indicate change in risk over time. This is a quantitative molecular test, with a linear range of 500-39,000,000 copies/mL.

TC# 102-3012

Processing: Centrifuge and aliquot into Referred Tests aliquot tube within 2 hours of collection. Freeze Tranportation: Transport frozen on ice AWL Core Lab: Please deliver to Referred Tests when received on day shift.

Preferred Collection/Volume: Random urine collected in sterile container Volume: 1.0mL u

TAT: 4 days Test Schedule: Tuesday and Thursday mornings BK Virus DNA, Quant Test Method: Real-Time Polymerase Chain Reaction (RT-PCR) RT PCR Urine Testing Location: Oregon Health Sciences University Portland, Oregon

Clinical Significance This test is used to determine the presence of BK virus DNA in patient's specimens. Detection of the virus in these specimens may be indicative of an active infection, as PCR detects the presence of the virus, and not the host's reaction to the virus. Polyomavirus BK

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DNA detection in urine is associated with an increased risk of graft rejection in renal recipients.

TC# 102-3012

Processing: Freeze sample upon receipt Transportation: Tranpsort frozen on ice AWL Core Lab: Please deliver to Referred Tests when received on day shift.

Preferred Collection: Adult: One aerobic and one anaerobic blood culture bottle Pediatric: One Pediatric blood cutlure bottle Volume: Adult: 8-10 mL each bottle Pediatric: 1-4 mL Note: Please refer to work instruction Blood Specimen Collection: Blood Culture #2.2-08-204

TAT: 5 - 10 Days Test Schedule: Daily Test Method: Culture Testing Location: Airportway Regional Laboratory, Sunnyside and Westside Medical Centers

Blood Culture Clinical Data: If limited draw, collect aerobic bottle only. For pediatric patients; if anaerobic organism are suspected, collect an additional adult anaerobic bottle. Bone Marrow: rarely indicated for routine bacteriology. Consult with Microbiology Director or Technical Specialist. Patient History: n/a

Rejection Criteria: Expired blood culture bottle Greater than 24 hours post collection

Processing: Hold at room temperature Transport: Room temperature Note: Transport to the testing lab as soon as possible

Preferred Collection: Heparinized syringe Volume: 3.0mL wb (Absolute Minimum Volume: 0.2mL wb)

TAT: STAT Test Schedule: Daily Method: Ion Selective Electrode Test Facility: INT, LVK, NLR, SKY, SMC, WMC

Blood Gas Arterial Note: Arterial blood gasses: Heparinized syringe (NOT COLLECTED BY LAB STAFF) For Venous specimens, see panel Blood Gas Venous. For Capillary specimens, see panel Blood Gas Capillary.

Processing: Needle must be removed and syringe capped, analyze within 30 minutes of collection. Transport: Transport at room temperature Rejection Criteria: Air bubbles, age of specimen

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Preferred Collection: Capillary tube, heparinized Volume: 1.0 mL wb (Absolute Minimum Volume: 0.2 mL wb)

TAT: STAT Test Schedule: Daily Method: Ion Selective Eletrode Test Facility: INT, LVK, NLR, SKY, SMC, WMC Blood Gas Capillary Note: For arterial specimens, see panel Blood Gas. For venous specimens, see panel Blood Gas Venous.

Processing: Analyze within 10 minutes of collection Rejection Criteria: Inadequately filled specimens, air bubbles, visible clots, age of specimen

Preferred Collection: Heparinized Syringe (only when specimen is colleted at testing location) Minimum Volume: 0.3 mL Alternative Collection: GRN Li Hep Note: Tube Must Be Full Volume: 3.0 mL wb Note: NO gel barrier tubes for collection

TAT: TAT Test Schedule: Daily Method: Ion Selective Electrode Blood Gas Venous Testing Location: INT, LVK, NLR, SKY, SMC, WMC Rejection Criteria: Inadequately filled tubes, age of specimen

Note: Central or mixed venous samples should be collected in a blood gas syringe. Peripheral venous samples may be collected in a blood gas syringe for testing at the collection site only or a filled green lithium heparin top tube without gel barrier. Do not transfer samples from tubes to syringes or vice versa. Keep samples sealed and free from exposure to air. For arterial specimens, see panel Blood Gas. For capillary specimens, see panel Blood Gas Capillary.

Processing: DO NOT UNCAP Transport: Transport at room temperature. If not tested within 2 hrs., refrigerate.

Preferred Collection: Capillary tube, heparinized Volume: 1.0 mL cb (Absolute Minimum Volume: 0.2 mL cb)

TAT: STAT Blood Gas, Venous, Test Schedule: Daily Cord Blood Method: Ion Selective Electrode Test Facility: Sunnyside and Westside Medical Centers

Processing: Fill tube completely and test within 10 min. DO NOT ICE.

Preferred Collection: Capillary tube, heparinized Volume: 1.0 mL cb (Absolute Minimum Volume: 0.2 mL cb)

TAT: STAT Test Schedule: Daily

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Method: Ion Selective Electrode Test Facility: Sunnyside and Westside Medical Centers

Note: Intended for point-of-care neonatal blood gas testing, performed by Labor Blood Gases, Arterial, and Delivery nursing staff at KSC. Cord Blood Processing: Fill tube completely and test within 10 min. DO NOT ICE. Rejection Criteria: Air bubbles, age of specimen

Preferred Collection/Volume: Body Fluid inoculated into Blood Culture bottles: One aerobic and one anaerobic bottle / 1 to 10 mLs each bottle. An additional specimen for Gram Stain must also be submitted, place >0.5mL in a sterile leakproof container and submit with bottle. Alternative Collection: Sterile leakproof container/ > 1 mL; The specimen may be inoculated into a SPS tube. SPS Tube must be completely filled with specimen.

TAT: 5 - 14 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: Acceptable sources: Amniotic, Ascites, Biliary, Dialysis, Synovial, Paracentesis, Body Fluid Culture Pericardial, Pleural. If fluid is not one of these sources, refer to Abscess/Aspirate Culture Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Greater than 24 hours post collection. Expired collection/transport containers. SPS tube less than maximum fill. Any additive tube other than SPS.

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: 1 mL or more of aspirate Alternative Collection: n/a

TAT: 2 -3 working days Test Schedule : Any day Test Method: n/a Test Facility: Airport Way Laboratory

Clinical Data: n/a Patient History: n/a Bone Marrow Prep Patient Preparation: Physician will explain the procedure to the patient. Labeling: n/a

Clinician or pathologist may instruct lab to send specimen to OHSU for flow cytometry on weekends and holidays.

Processing; Refer to WIs 2.2 -15-200, Bone Marrow Specimen Handling. Transport: Refer to WIs 2.2 -15-200, Bone Marrow Specimen Handling

Preferred Collection/Volume: RED 10

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Volume: 1.0 mL Serum Alternative Collection: RED SST Volume: 1.0 mL Serum

Test Schedule: Mon - Fri Method: Multiple Analyte Immuno Detection (MAID) Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave, Suite 200 Cypress, CA 90630

Clinical Data: Levels of antibody recognizing pertussis toxin (PT) and filamentous antigen (FHA) are typically increased following either vaccination or natural Bordetella Pertussis exposure to Bordetella pertussis. Detection of IgG antibody is more sensitive than detection of IgA Antibody antibody, since not all vaccinated or exposed individuals mount a detectable IgA response. Increased levels of FHA antibody alone may represent cross-reactive antibody induced by infection with other Bordetella species, Mycoplasma pneumoniae, Chlamydia pneumoniae, or nonencapsulated Haemophilus influenzae .

Note: Testing performed by FOCUS via Quest Chantilly Specimen Stability: Room temperature: 7 days Refrigerated: 14 days Frozen: 30 days Test Code: Quest TC 12966

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: This test is no longer available per the Infectious disease department.

This test is no longer available per the Infectious Disease Department. Bordetella Pertussis Culture. This test is no longer available per the Infectious Disease Department. NO TEST CODE

This test is no longer available per the Infectious Disease Department.

Preferred Collection: Nasopharyngeal (np) swab collected with Green top flexible wire shaft placed back into swab container with liquid Amies.

TAT: 1-3 days Test Schedule: Daily Method: Loop-Mediated Isothermal Amplification (LAMP) Molecular Technology Bordetella Pertussis Test Facility: AWL Infectious Diseases Molecular Laboratory PCR Specimen Stability: Room Temperature: 5 Days Refrigerated: 7 Days Do Not Freeze

Transport: Transport in red tote at refrigerated temperature to AWL

Preferred Collection/Volume: Collection kits available on request (503-693-4114). Infant Botulism: Stool sample collected in clean container (no transport media) Volume: approximately 2.0 oz. stool Adult Botulism: Stool sample and RED10 Volume: approximately 2.0 oz. stool colected in clean container (no transport media) AND 5.0 mL sserum Wound Botulism: specimen from wound in anaerobe transport media, stool AND RED10 Volume: approximately 2.0 oz. stool collected in clean contaienr (no transport media) AND 5.0 mL

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serum Food: All remaining suspect food package including brand information, lot numbers and expiration date

TAT: 4 to 10 working days Test Schedule: Daily, on regular business days Method: Standard FDA and Laboratory Response Network (LRN) reference procedures; mouse neutralization assay Test Facility: Public Health Division 800 NE Oregon Street Portland, OR 97232 Botulism Toxin Assay Complete Oregon Health Department requisition. Tranportation: Transport samples on ice. Rejection Criteria: Leaking containers, unlabeled specimens, specimens submitted with incomplete requisition forms or specimens submitted without state or local health department authorization will not be processed.

Processing: Centrifuge and aliqout serum. Transport: Transport all samples on ice.

Preferred Collection/Volume: LAV EDTA Volume: 6-10 mL wb Alternative Collection: Yellow ACD ***Collect samples Sunday through Thursday only. Must ship to testing location and arrive within 72 hours of collection.

TAT: 7-10 days Test Schedule: Test Method: Deletion/Duplication Analysis Gene Sequence Analysis Specific Mutation Analysis Test Facility: Ambry Genetics 15 Argonaut Aliso Viejo, CA BRCA Ashkenazi 92656 Multisite 3 Phone (949) 900-5500

Test Description BRCA1 coding exons 1-22, BRCA2 coding exons 1-26, plus at least 5 bases into the 5’ and 3’ ends of all the introns are analyzed. Genomic deoxyribonucleic acid (gDNA) is isolated from the patient’s specimen using standardized methodology and quantified using a spectrophotometer. Sequence enrichment is carried out by incorporating the gDNA into microdroplets along with primer pairs designed to the target BRCA1 and BRCA2 coding exons followed by polymerase chain reaction (PCR) and Next-Generation sequencing. Follow-up sequencing is performed for any regions missing or with insufficient read depth coverage for reliable heterozygous variant detection. Suspect variant calls other than benign alterations are verified by a second sequencing methodology. Comprehensive (full-gene) gross deletion/duplication analysis using the multiplex ligation-dependent probe amplification (MLPA) kits P002-C2 (BRCA1) and P045-B3 (BRCA2), developed by MRC Holland, is also performed. Sequence analysis is based on the following NCBI reference sequences: BRCA1 - NM_007294 and BRCA2 - NM_000059. Mutation Detection Rate:

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Analytical sensitivity for all genes is estimated to be greater than 99% of described mutations.

Stability: Room Temperature Shipped within 72hours

Processing: Do not centrifuge. Transport: Transport to AWL in blue tote

Preferred Collection: LAV EDTA(K3) from kit Volume: 6-10 mL wb

Test Facility: Ambry Genetics Corporation 15 Argonaut Aliso Viejo, CA 92656

Patient Preparation: Patient will present with Ambry kit for collection. Collect specimen as directed by kit Breast Cancer 1 Gene instructions. Mutation Analysis Note: If there is no Ambry collection kit available, please collect specimen in 2 LAV EDTA tubes.

Note: Requires clinical Genetics approval

Processing: Do not centrifuge. Transport: Room temperature NOTE: Specimen must be sent with the entire kit to Referred Tests, do not place labels on the outside of the test kit.

Preferred Collection: LAV EDTA(K3) from kit Volume: 6-10 mL wb

Test Facility: Ambry Genetics Corporation 15 Argonaut Aliso Viejo, CA 92656

Patient Preparation: Breast Cancer 2 Gene Patient will present with Ambry kit for collection. Collect specimen as directed by kit

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Mutation Analysis instructions. Note: If there is no Ambry collection kit available, please collect specimen in 2 LAV EDTA tubes.

Note: Requires clinical Genetics approval

Processing: Do not centrifuge. Transport: Room temperature NOTE: Specimen must be sent with the entire kit to Referred Tests, do not place labels on the outside of the test kit.

Preferred Collection: LAV EDTA(K3) from kit Volume: 6-10 mL wb

Test Facility: Ambry Genetics Corporation 15 Argonaut Aliso Viejo, CA 92656

Patient Preparation: Patient will present with Ambry kit for collection. Collect specimen as directed by kit instructions. Breast Cancer BRCA Note: If there is no Ambry collection kit available, please collect specimen in 2 LAV 1,2 Mutation Analysis EDTA tubes.

Note: Requires clinical Genetics approval

Processing: Do not centrifuge. Transport: Room temperature NOTE: Specimen must be sent with the entire kit to Referred Tests, do not place labels on the outside of the test kit.

Preferred Collection/Volume: O ne tissue block with both the greatest amount/area of invasive breast /associated stroma and the least amount of non-invasive mammary (in situ carcinoma, hyperplastic epithelium, normal epithelium).

TAT: 7 to 10 days Test Schedule: Mon-Fri Breast Cancer Oncotype Test Facility: Genomic Health Inc, Recurrence Score 301 Penobscot Dr. Redwood City, CA 94063

Clinical Data: The Oncotype DX breast cancer assay, is a test that examines a breast cancer patient's tumor tissue at a molecular level, and gives information about a patient's individual disease. This information can help individualize breast cancer treatment planning and identify options. The Oncotype DX breast cancer test is the only multigene expression test commercially available that has clinical evidence validating its ability to predict the likelihood of chemotherapy

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benefit as well as recurrence in early-stage breast cancer. The Oncotype DX gene expression assay is intended to be used by women with early-stage (stage I or II), node-negative, estrogen receptor-positive (ER+) invasive breast cancer who will be treated with hormone therapy.

Labeling: All specimens must be labeled with S barcode labels from the Onco type DX Specimen Collection and Transportation Kit Box for that patient.

Specimen Stability: Room Temp OK Refrigerated OK

Transport: OncoType kit provided by Genomic Health

Preferred Collection/Volume: Dark Blue Trace Metal Free No Additive Volume: 2.0 mL s

TAT: Report available: 3 Days Test Schedule: Monday, Wednesday, Friday AM Method: Colorimetric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Bromide Level Specimen Stability: Room Temperature: 72 hours Refrigerated: Not Established Frozen: Not Established TC# 0632

Processing: Centrifuge and aliquot info trace metal free Referred Tests aliquot tube. Transport: Send Room Temperature

Preferred Collection/Volume: BAL in sterile leakproof container/ >=1mL. Bronchial Brush in sterile leakproof container with 1-5 mLs sterile salines. Alternative Collection: n/a

TAT: 2 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: Indicate specific collection site in source field. Bronchial Alveolar Patient History: n/a Lavage Culture Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Specimen greater than 24 hours post collection.

Processing: Seal transport container with parafilm. Refrigerate. Transport: Send on Ice

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5 mL p Alternative Collection: RED SST or serum acceptable BUN Volume: 0.2 mL s

TAT: 1 HR STAT / 24 HR ROUTINE Test Schedule: Daily

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Method: Photometric, Rate Test Facility: SMC, AWL, INT, ALL CLINICS

Processing: Centrifuge. Refrigerate if held. Transport: iSTAT Testing Performed by LAB Staff: Whole blood from unspun GNS5 acceptable for testing.

Preferred Collection/Volume: LAV EDTA Volume: 1.0 mL Plasma Alternative Collection: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 2 days Test Schedule: Wednesday, Sunday Morning Method: High Performance Liquid Chromatography/Tandem Mass Spectrometry Test Facility: NMS LABS 3701 Welsh Road Willow Grove, PA 19090 Level, LC MS/MS Clinical Data: Bupropion is an antidepressant for treating mental depression. Monitoring its level is important to optimize therapy, to avoid toxicity, and to assure compliance.

NOTE: Test performed at NMS via Quest Chantilly. Specimen Stability: Room Temperature: Not Stable Refrigerated: Not Stable Frozen (-20 °C): 1 month(s) Test Code: QUEST TC 14367

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Note if serum or plasma on specimen.Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 3.0 mL Serum

TAT: Report available: 4 Days Test Schedule: Monday-Friday Afternoon Method: Gas Chromatography Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Butalbital Chantilly, VA 20153 Specimen Stability: Room Temperature: 5 days Refrigerated: 7 days Frozen: 1 month (-20c) Test Code: CHANTI LLY TC 22642

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: Submit a single, freshly passed, liquid fecal specimen.

TAT: STAT: If performed, C. diff PCR reported within 2 hours of receipt. Test Schedule: Sun - Sat Method: Real Time PCR Test Facility: NWMC Laboratories

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Clinical Data: This is a reflex from C. difficile toxin antigen test. Not separately orderable. C Diff PCR Rejection Criteria: Solid stool.

Transport: Transport at 2 - 8 C. Specimens are stable for 72 hours at 2 - 8 C.

Preferred Collection: RED 10 or Serum Acceptable Volume: 1.0 mL serum

TAT: Next Day Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 C1 Esterase Inhibitor TC# 298

Specimen Stability Room temperature: 7 days Refrigerated: 8 days Frozen: 1 year http://www.questdiagnostics.com/testcenter/TestCenterHome.action

Processing: Centrifuge and aliquot into a referred tests aliquot tube. Transport: Room Temperature

Preferred Collection/Volume: RED10 No Gel Barrier Tubes Volume: 1.0 mL s

TAT: Next Day Test Schedule: Tuesday and Fridays P.M. Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

C1 Esterase Inhibitor, Clinical Data: C1 Esterase is decreased in angioedema. The inherited form is usually Functional diagnosed in the first two decades of life. The acquired form affects primarily adults with autoimmune or lymphoproliferative disorders. Approximately 15% of patients with hereditary angioedema have a normal concentration of the protein but it is dysfunctional.

Reject Criteria: Lipemia Received thawed Received room temperature Received refrigerated Serum Separator Tube (SST ® ) Glass tubes CSF

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Specimen Stability: Room temperature : Unacceptable Refrigerated: Unacceptable Frozen: 30 days TC# 23042

Processing: Centrifuge and aliquot into Referred Tests aliquot tube within one hour of collection. Freeze. Transport: Transport frozen on ice

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: 4 Days Test Schedule: 2 Days a week. Method: Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130 C1q Complete Component Clinical Data: The complement system is critical to the inflammatory response. C1q concentrations may be decreased in patients with acquired angioedema, immune complexed induced vasculitis, and concurrent low concentrations of C1 inhibitor, carcinoma, or lymphoma.

Specimen Stability: Room Temperature: 2 days Refrigerated: 2 weeks Frozen: 1 month Test Code: SJC TC 981X

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: Plasma is not acceptable. RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Tuesday, Thursday Morning Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 C1q Immune Complex Detection Clinical Data: Assay detects circulating immune complexes, which are capable of activating the classical complement pathway.

Specimen Stability: Room Temperature: Unacceptable Refrigerated: 48 hours Frozen: 1 year Test Code: CHANTILLY TC 36735

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze serum within 1 hour of time drawn. Do not submit the sample in a glass tube. Do not thaw. Transport: Transport frozen on ice

Preferred Collection/Volume: RED 10

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No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume 1.0 mL Plasma

TAT: Report available: 3-4 Days Test Schedule: Monday, Wednesday Night Method: Radial Immunodiffusion Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

C2 Complement Clinical Data: The complement system is critical to inflammatory responses. C2 is a component of the classical pathway. Decreased concentrations are observed in patients with immune complex diseases, such as systemic lupus erythematosus (SLE) and immune complex- induced vasculitis.

Specimen Stability: Room Temperature: 2 days Refrigerated: 2 weeks Frozen: 1 month Test Code: SJC TC 433X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube Transport: Refrigerate if held

Preferred Collection/Volume: RD10/0.5mL s Alternative Collection: 0.2mL s

TAT: 24 HR Test Schedule: DAILY C3 Complement Method: Turbidometry Test Facility: AWL

Processing: Cent. Aliquot. Refrigerate if held.

Preferred Collection/Volume: RD10/0.5mL s Alternative Collection: 0.2mL s

TAT: 24 HR Test Schedule: DAILY C4 Complement Method: Turbidometry Test Facility: AWL

Processing: Cent. Aliquot. Refrigerate if held

Preferred Collection: RED10 Volume: 1.5mL s Alternative Collection: CA 125 GRN Li Hep Volume: 1.0mL p

TAT: 24 HR Test Schedule: Daily

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Processing: Centrifuge and aliquot. Refrigerate if held. Freeze if held greater than 48 hours.

Preferred Collection/Volume: RED SS5 Volume: 1.0mL s Absolute Minimum: .05mL s Amniotic fluid, synovial fluid, csyt fluid, pericardial fluid, and vitrous fluid are unacceptable for this test. Plasma is unacceptable.

TAT: Next Day Test Schedule: Monday-Saturday AM and PM Test Method: Immunoassay (IA) Performing Laboratory Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Limitations Benign diseases of the breast and liver may cause elevated results.

Clinical Significance CA 15-3 may be useful for monitoring patients with metastatic breast cancer and certain ovarian cancers. The CA 15-3 values from sequential samples have a high correlation with the clinical course in most patients with metastatic breast cancer. CA 15-3 Alternative Names(s) Cancer Antigen 15-3

Transport Temperature Room temperature Specimen Stability Room temperature: 7 days Refrigerated: 7 days Frozen: 28 days Reject Criteria Gross hemolysis • Gross lipemia TC# 5819x

Reference Range(s) CA 15-3 <32 U/mL This test was performed using the Siemens (Bayer) Chemiluminescent method. Values obtained from different assay methods cannot be used interchangeably. CA 15-3 levels, regardless of value, should not be interpreted as absolute evidence of the presence or absence of disease.

Specimen Processing: Centrifuge Transportation: Transport to AWL in blue tote

Preferred Collection/Volume: RED10 Volume: 1mL s

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TAT: Next Day Test Schedule: Monday-Friday All Shifts Saturday Morning, Night Sunday Night Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

N/A

Clinical Significance A large percentage of patients with gastrointestinal tumors (such as pancreatic, liver, gastric, colorectal tumors) and some other malignancies have been shown to have elevated CA 19-9 serum CA 19-9 levels. Serum CA 19-9 levels may be useful for monitoring disease activity or predicting relapse following treatment. CA 19-9 should not be used as a screening test. Alternative Names(s) Carbohydrate Antigen 19-9

Specimen Stability: Room temperature: 7 days Refrigerated: 7 days Frozen: 28 days TC# 4698

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transportation: Transport to AWL in blue tote. Refrigerate if held.

Collection Instructions Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Methodology Immunoassay (IA) Performing Laboratory Quest Diagnostics Nichols Institute 14225 Newbrook Drive CA 27.29 Chantilly, VA 20153 Setup Days Monday-Saturday Morning, Night

Clinical Significance CA 27.29 may be useful for monitoring patients for metastatic breast cancer.

Specimen Stability Room Temperature: 7 days Refrigerated: 7 days Frozen: 28 days

Specimen Handling

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Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Blue tote to AWL

Preferred Collection/Volume: 24 Urine in Acid Washed Container Volume 7.0 mL urine Preserve 24-hour urine with 25 mL 50% Acetic Acid during collection or collect without preservative.

TAT: Report available: Next Day Test Schedule: Tuesday, Thursday Morning Method: Inductively Coupled Plasma-Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Cadmium 24 Hour Urine Patient Preparation: Patient should refrain from eating seafood at least 3 days before specimen collection.

Specimen Stability: Room Temperature: 5 days Refrigerated: 1 week Frozen: 2 weeks Test Code: CHANTILLY TC 9806

Processing: n/a Transport: Refrigerated

Preferred Collection/Volume: DarkBlue EDTA Volume 4.0 mL Whole Blood

TAT: Report available: 2 Days Test Schedule: Monday-Friday Afternoon Method: Inductively Coupled Plasma-Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Cadmium is a naturally occurring element that is mined and used in industrial Cadmium Level production because of its durability. Excessive cadmium exposure can damage lungs, kidneys, and the digestive tract.

Specimen Stability: Room Temperature: 48 Hours Refrigerated: 5 Days Frozen: Unacceptable Test Code: CHANTILLY TC 299

Processing: Do not centrifuge Transport: Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Mon-Fri Afternoon Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

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Clinical Data: concentration is increased in patients with medullary thyroid carcinoma. Calcitonin concentrations may be used to monitor disease.

Patient Preparation: Overnight fasting is preferred

Specimen Stability: Calcitonin Level Room temperature: Unacceptable Refrigerated: 24 hours Frozen: 28 days Test Code: CHANTILLY TC 3822

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze solid. Transport: Transport frozen on ice

Preferred Collection/Volume: 24 Hour Urine and Grn Li Hep PST Collect creatinine and calcium blood specimens upon return of 24 hour urine collection. **Blood specimens may be collected up to 24 hours prior to submission of urine. Patient should refrigerate urine during collection. Volume: 4.0 mL p 4.0mL u

Preferred Collection/Volume: GNS5, 24 Hr Urine/4.0 mL p/u (plasma or urine?) Alternative Collection: 1.0 mL p/u/12x75 aliquot

TAT: 24 HR Test Schedule: Daily Calcium Fractional Method: Colorimetric, Arsenazo III Excretion 24 Hour Test Facility: AWL Urine Collection is for 24 hour urine and plasma creatinine and calcium. Do not walk in orders creatinine and calcium plasma specimens If patient presents for blood specimen collection prior to submission of 24 hour urine.

Calcium Fractional Excretion is a 24 hour urine sample with a plasma calcium and creatinine. Plasma samples should be collected at same time as 24 hour Urine submission or within 24 hours of collection.

Processing: Determine pH and adjust if needed to 4-5 with 6n HCl. Mix sample well and aliquot 4.0 mL urine into 12x75 aliquot tube. Submit Grn Li Hep PS for creatinine and calcium with urine. Note on urine sample total volume and preservative used during collection.

Preferred Collection/Volume: GNS5 plus AP99/4.0 mL p/u Alternative Collection: 1.0 mL p/u/12x75 aliquot

TAT: 24 HR Test Schedule: Daily Method: Colorimetric, Arsenazo III Calcium Fractional Test Facility: AWL Excretion Random Calcium Fractional Excretion combines with Calcium Excretion Serum (serum calcium and serum creatinine). Serum should be collected at the same time as urine is submitted or within 24 hours or urine collection.

Processing: Adjust pH to 4-5 with 6N HCl. Aliquot into 12x75 tube. Refrigerate if held.

Preferred Collection/Volume: 4.0mL u/24 hr Urine

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Alternative Collection: 1.0mL u/12x75 aliq.

TAT: 24 HR Test Schedule: Daily Method: Colorimetric, Arsenazo III Calcium Level 24 Hour Test Facility: SMC, AWL, BVT Urine For random urine see panel Calcium level urine. Refrigerate during collection. Adjust pH to 4-5 with 6N HCl when specimen received before aliquoting.

Processing: Record collection interval hrs. Measure and record total vol. Adjust pH to 4-5. Aliquot into 12x75 tube. Refrigerate if held.

Medical Center Locations Preferred Collection: Drk GRN Li Hep or Heparinized Syringe Alternative Collection: GRN Li Hep PST Medical Office Locations Preferred Collection: Drk GRN Li Hep Volume: 3.0 mL wb Drk GRN Li Hep or 1.0mL Heparinized Syringe (Absolute Minimum Volume: 1.0 mL wb Drk GRN Li Hep or 0.2mL Heparinized Syringe) Note: If specimen is collected in a Drk GRN Li Hep or GRN Li Hep PST, do not open prior to testing.

TAT: STAT 1 Hour Routine 6 hours Test Schedule: Daily Test Method: Ion Selective Electrode Calcium Level Ionized Testing Location: SMC and WMC Specimen Stability: Heparinized Syringe 30 minutes Drk GRN Li Hep or GRN Li Hep PST 6 hours if refrigerated

Note: STAT testing is only available for specimens collected at SMC and WMC.

Processing Information: Do not centrifuge Transport Temperature: Refrigerated Rejection Criteria: Inadequately filled specimens, air bubbles, visible clots or age of specimen Note: If specimen is refrigerated allow specimen to come to room temperature before testing. Centrifuged specimens may be tested if tube has not been opened prior to testing

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p Alternative Collection: RED SST or serum acceptable Volume: 0.2mL s

TAT: 1 Hr / 24 Hr Calcium Level Total Test Schedule: Daily Method: Colorimetric, Arsenazo III Test Facility: SMC, INT, AWL, BVT, CAS, LVK

Processing: Cent. Refrigerate if held

Preferred Collection/Volume: Random urine specimen submitted in sterile container Volume : 4.0mL u Alternative Collection: 1.0mL u

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TAT: 24 HR Test Schedule: Daily Method: Colorimetric, Arsenazo III Test Facility: SMC, AWL, BVT Calcium Level Urine Random urine specimen for calcium. Adjust pH to 4-5 with 6N HCl

Processing: Random urine sample. Adjust pH to 4-5 w 6N HCl upon receipt. Tranpsortation: Transport to AWL. Refrigerate if held.

Preferred Collection/Volume: Undiluted feces collected in sterile leak proof container. Do not add fixative or preservative. Volume: 1.0g

TAT: 3 days Test Schedule: Set up Monday and Thursday Mornings Test Method: Immunoassay Testing Location: Performing Laboratory Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Significance Diagnose inflammatory bowel disease (IBD), including Crohn's disease and ulcerative Calprotectin, Stool colitis. Differentiate IBD from irritable bowel syndrome (IBS). Limitations NSAIDs may cause elevated results.

Specimen Stability Room temperature: 11 days Refrigerated: 11 days Frozen: 1 year TC# 16796 This test only performed at Quest, SJC

Processing: Divide sample if multiple tests are ordered and performed at different locations. Freeze upon receipt. Transportation: Transport frozen on ice.

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL Whole blood

Test Facility: Kaiser Regional Molecular Genetics Lab 5755 Cottle Rd. Bldg #6 San Jose, CA 95123 (408)972-3308. Canavan Disease Mutation Analysis Refrigerate if held overnight. Ship Room Temperature

Processing: Do not Centrifuge. Transport: Send Room Temperature

Preferred Collection/Volume: AP99 Random Urine/50 mL u Alternative Collection: 10 mL u

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TAT: 24 HR Test Schedule: Daily Method: Immunoassay Cannabinoid Screen Test Facility: AWL Urine Included in drug screen panels Drug Screen Abbreviated Basic Comprehensive.

Processing: Refrigerate if held overnight.

Preferred Collection/Volume: AP99 Random Urine/50 mL u Alternative Collection: 10 mL u

TAT: 24 HR Test Schedule: M-F Method: GC/MS Cannabinoids, Urine, Test Facility: AWL Confirmatory ICN ordered from OHSU only. To order a drug screen see panels Drug Screen Abbreviated Basic Comprehensive.

Processing: Refrigerate if held overnight.

Preferred Collection/Volume: GRN Li Hep 4 NO gel barrier Volume: 0.5 mL p/s Alternative Collection: RED 10 Absolute Minimum Volume: 0.2 mL p/s

TAT: 1 HR / 24 HR Carbamazepine Level Test Schedule: Daily Method: Enzyme Immunoassay Test Facility: SMC, AWL

Preferred draw time: trough - immediately before next dose. Gel barrier tubes NOT acceptable.

Processing: Cent. Aliquot. Refrigerate

Preferred Collection/Volume: GRN Li Hep PST Volume: 1.5mL p Alternative Collection: RED SST

TAT: 1 HR / 24 HR Test Schedule: Daily Method: Photometric, Enzymatic Test Facility: Sunnyside Medical Center Westside Medical Center Airport Way Regional Laboratory Carbon Dioxide Level Interstate Medical Office Beaverton Medical Office Cascade Park Medical Office Longview Kelso Medical Office Skyline Medical Office

Fill tube completely. Do Not aliquot or send an aliquot for testing. STAT requests at North Lancaster Medical Office: test whole blood on iSTAT, recap sample and proceed with usual processing instructions.

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Processing: Centrifuge. DO NOT REMOVE CAP. Refrigerate if held. Add-on testing stability: 72 hours if specimen has remained capped.

Preferred Collection: GRN Li Hep **Tube Must Be Full** Alternative Collection: Heparin syringe/capillary tube Volume: 4.0mL wb (Absolute Minimum Volume: 0.5mL wb)

TAT: 1 HR STAT Test Schedule: Daily Method: Co-oximetry Carboxyhemoglobin Test Facility: Sunnyside Medical Center and Westside Medical Center Level Note: COHb stable if stored in filled, well capped tube. If drawn in syringe or capillary tube air bubbles must be expelled immediately. Securely cap and mix syringes. Cap capillary tubes with rubber caps. Heparin syringe/capillary tube (0.5 mL min.).

Processing: Do not centrifuge. Refrigerate

Preferred Collection/Volume: RD10/1.0 mL bf Alternative Collection: LIGREEN4/0.5 mL bf

TAT: 4 days Test Schedule: Tue & Fri Carcinoembryonic Method: Chemiluminescent Immunoassay Antigen Body Fluid Test Facility: AWL

Intended for cyst fluid

Processing: Refrigerate if held.

Preferred Collection: RED SST Volume: 1.0 mL serum Alternative Collection: RED 10 Minimum Volume: 0.5 mL serum

TAT: 1-3 Days Cardiolip Beta2-Glyco Testing Schedule: Monday -Friday G,M Method: Mutiplex Immunoassay Testing Location: Airportway Laboratory

Note: Includes Cardiolipin IgG and IgM and Beta 2 -Glycoprotein IgG and IgM.

Processing: Centrifuge, aliquot (if collected in a RED 10), refrigerate.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: GRN NA HEP 4 Volume:1.0 mL Plasma

TAT: Report available: 5 Days Test Schedule: Monday, Wednesday, Friday, Saturday Afternoon

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Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Serum carnitine analysis is useful in the diagnosis and monitoring of patients with either primary or secondary carnitine deficiency.

Carnitine Free and Patient Preparation: Patient should be well fed. Total Specimen Stability: Room Temperature: 5 hours Refrigerated: 5 days Frozen: 1 month Test Code: SJC TC 70107X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze solid. Transport: Transport frozen on ice.

Preferred Collection/Volume : Random urine in AP99 Volume: 3.0 mL Urine

TAT: Report Available: 4 days Test Schedule: Thursday; 8 a.m. Method: Flow Injections Analysis-Tandem Mass Spectrometry (FIA-MS/MS) Test Facility: Mayo Medical Laboratories 200 First Street SW Carnitine Free and Rochester, MN 55905 Total Urine Specimen Stability: Room Temperature: Unacceptable Refrigerated: 72 hours Frozen: 1 Month Test Code: Quest TC 30338X TO Mayo TC 81123

Processing: Aliquot into Referrred Tests aliquot tube and freeze specimen. Transport: Transport frozen on ice.

Preferred Collection/Volume: RED10 Protected from light Alternate Collection: REDSST Protected from light Volume : 2.0 mL s

TAT: Report available: 3 Days Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Carotene Level Clinical Data: Beta Carotene, a fat soluble nutrient, is a precursor to vitamin A. Deficiencies may lead to vitamin A deficiency. Excessive vitamin A intake may lead to headaches, loss of appetite, nausea and diarrhea, skin changes, and potential birth defects.

Specimen Stability: Room Temperature: 3 Days Refrigerated: 6 Days Frozen: 1 Year TC# 0722

Processing: Centrifuge and aliquot into amber aliquot tube. Use foil to protect from light if amber tubes are not available. Transport: Refrigerate if held

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Preferred Collection/Volume: Patients should be relaxed in either a supine or upright position before blood is drawn. No EDTA Acceptable Preferred Specimen: GREEN NA HEP 6 (pre-chilled) Volume: 4.0 mL Plasma

TAT: Report available: 2 Days Test Schedule: Monday, Wednesday, Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: The evaluation of plasma is utilized in the differential diagnosis of . In addition, monitoring levels in association with clonidine Catecholamines suppression has been recommended as a means of distinguishing patients with pheochromocytoma from patients with essential hypertension. Measurement of plasma Fractionated norepinephrine levels may aid in the differential diagnosis of orthostatic and postural hypotension.

Patient Preparation: Patient should avoid alcohol, coffee, tea, tobacco, and strenuous exercise before specimen collection. Overnight fasting is required. States of anxiety and stress can cause fluctuations in levels.

Specimen Stability: Room Temperature: 6 HOURS Refrigerated: 6 HOURS Frozen: 1 MONTH Test Code: CHANTILLY TC 14566

Processing: Separate plasma in a refrigerated centrifuge within 30 minutes of collection. Transfer plasma to clean, plastic, screw-capped vial(s), freeze immediately. Do not thaw Transport: Transport frozen on ice

Preferred Collection/Volume: Collect 24-hour urine with 25 mL 6N HCl to maintain a pH below 3. Urine with preservative should maintain a pH below 3. Urine without preservative is acceptable if pH is below 6 and the sample is shipped frozen. NO Boric Acid and Acetic Acid 24 hour Urine Container Volume: 10.0 mL 24-Hour Urine in leak-proof container

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Catecholamines Chantilly, VA 20153 Fractionated 24 Hour Urine Clinical Data: The three catecholamines (norepinephrine, epinephrine, and ) are the principal secretory products of neural tissue. Clinically, the measurement of circulating catecholamines is valuable in the diagnosis of catecholamine secreting tumors associated chiefly with hypertension (, , and gangliomas) and with the evaluation of orthostatic hypotension.

Patient Preparation: Clinician Instructions ONLY: It is preferable for the patient to be off medications for a minimum of 18-24 hours prior to collection however, common antihypertensives (diuretics, ACE inhibitors, calcium channel blockers, alpha and beta blockers) may cause minimal or no interference. Patient should avoid tobacco, tea, coffee, and strenuous exercise for 8-12 hours prior to collection.

Specimen Stability: Room Temperature: 7 Days Refrigerated: 30 Days

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Frozen: 49 Days Record perservative or pH and volume on specimen. Test Code: CHANTILLY TC 4646

Processing: Mix well and aliquot. Record perservative or pH and volume on specimen. Transport: Refrigerate if held

Preferred Collection/Volume: Dry sterile leakproof container/ 2-3 inch segment Alternative Collection: n/a

TAT: 3 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: Culture is for arterial or venous catheter tip. Urine catheter tip is unacceptable. Catheter Tip Culture Catheter is unsuitable for AFB or fungus culture. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Anything other than intravenous catheter.

Processing: Hold at room temperature Transport: Room Temperature

Preferred Collection: LAV EDTA Alternative Collection: LAV EDTA Microtainer Minimum Volume: 1 mL whole blood

TAT: 24 hours for routine specimens Testing Method: 5-Part diff Sysmex Hematology cell counter Stability:

{ Specimen is stable for 24 hrs at room temperature for all parameters.

CBC w/ Auto Diff { Refrigerated specimens are stable for 72 hours for hemogram (ABC) parameters.

{ Specimens 48-72 hrs old require evaluation of all abnormal differentials.

Rejection:

{ Clotted specimens

{ Hemolyzed specimens

{ IV contaminated specimens

Note: Test on site when possible is specimen is collected in a microtainer.

Processing: Handle microtainers ASAP and test on-site when possible

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Transport: Transport specimen to Airport Way Laboratory

Preferred Collection: LAV EDTA Minimum Volume: 3mL wb

TAT: 48hrs Test Schedule: Monday - Friday

Note: A CBC must be accompany this test CD4, T Cells, Absolute and Percent Note: Specimens must be collected by 1200PM (noon) on Fridays. All other days and times are acceptable for specimen collection.

Note: If CBC is not ordered, contact clinician to order a routine CBC.

Processing: Do not centrifuge Transport: Transport specimen at room temperature

Preferred Collection/Volume: RD10/1.0 mL s Alternative Collection: SSRED5/0.5 mL s

TAT: 4 days Test Schedule: Tue & Fri CEA Method: Chemiluminescent Immunoassay Test Facility: AWL

Processing: Cent, aliquot, refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 4 days Test Schedule: Monday-Friday Method: Bioassay Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave. Cypress, CA 90630

Patient Preparation: Specimens collected just before or within 15 minutes of the next antibiotic dose represent the trough levels. Cefoxitin Level Specimens obtained within 15-30 minutes after the end of I.V. infusion or 45-60 minutes after an IM injection or 90 minutes after oral intake represent the peak level. Labeling: Label tube as Peak or Trough

Specimen Stability : Room temperature: Unacceptable Refrigerated: Unacceptable Frozen: 30 Days Rejection Criteria: Serum separator tubes and all other fluids are unacceptable. Test Code: Quest TC 13243

Processing: Centrifuge and aliquot serum into referred test aliquot tube. Freeze Transport: Transport frozen on ice.

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Preferred Collection/Volume: Specimens collected just before or within 15 minutes of the next antibiotic dose represent the trough levels. Specimens obtained within 15-30 minutes after the end of I.V. infusion or 45-60 minutes after an IM injection or 90 minutes after oral intake represent the peak level. RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Reports in 1 day Test Schedule: Sets up 5 days a week, A.M. Method: Bioassay Test Facility: Focus Diagnostics, Inc. Ceftriaxone Level 5785 Corporate Ave. Cypress, CA 90630

Specimen Stability: Serum Room temperature: Unacceptable Refrigerated: Unacceptable Frozen: 30 Days Test Code: SJC TC 35158X TO FOCUS 51830

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Freeze. Transport: Frozen on ice

Preferred Collection/Volume: CSF submitted in sterile container or CSF Collection Tubes Volume: 1.0 mL CSF. Absolute Minimum 0.1 mL.

TAT: 1 hour Test Schedule: As needed Method: manual or automated cell count with manual differential Test Facility: Sunnyside and Westside Medical Centers Cell Count CSF Beaverton Medical Office Interstate Medical Office Longview Kelso Medical Office North Langcaster and Skyline Medical Offices

Processing: Cell count performed on site within one hour at designated testing locations. Use tube 4 unless indicated differently by clinician. Transport: Testing must occur within 1 hour of collection due to instability of cells. If unable to test on site, send STAT to testing site.

Preferred Collection/Volume: LV5 2.0 mL, minimum 0.5 mL. Clinician collected. Body fluid kits are supplied to clinical area by laboratory. Alternative Collection: heparin, no gel barriers

TAT: 4 hours Test Schedule: As needed Method: Manual or automated cell count with manual differential Cell Count w/ Diff Body Test Facility: BVT, INT, LVK, SMC, NLR, SKY Fluid Labeling: Add shared specimen label to sodium heparin tube if sharing with crystal analysis (joint fluids)

Processing: For joint fluids, cell counts may be shared with crystal analysis. Use sodium heparin tube for this. Label with shared specimen sticker. Transport: Testing must occur within 4 hours of collection. Send to testing site ASAP.

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Preferred Collection/Volume: CSF submitted in sterile container or CSF collection container Volume: . 5mL csf Alternative Collection: Syringe without needle

TAT: 3 - 5 Days 5 - 10 Days for Shunt specimen Test Schedule: Daily Method: Culture Test Facility: Airport Way Regional Laboratory Cerebrospinal Fluid Culture Clinical Data: Please indicate on specimen if source is shunt Testing tube is number 2 unless otherwise indicated Gram Stain will be performed STAT

Labeling: Please indicate collection tube number on Label

Rejection Criteria: Greater than 24 hours post collection

Processing: Use tube number 2 unless otherwise indicated by clinician. Transport: Transport immediately at room temperature to testing location.

Preferred Collection/Volume: SSRED5/0.5mL s/p Alternative Collection: RD10,GNS5/ 0.2mL s/p

TAT: 24 Hr Test Schedule: Daily Ceruloplasmin Method: Turbidometry Test Facility: AWL

Processing: Cent. Refrigerate if held.

Preferred Collection/Volume: Collect amd submit with blue swab from Aptima collection kit in Aptima transport media. Specimen sample is blue swabs of conjunctiva in Aptima transport system. If both white and blue swabs are submitted, sample is not rejected but will be resulted with a disclaimer.

TAT: Report available: 2 Days Test Schedule: Sun-Sat Method: Qualitative Nucleic Acid Probe Test Facility: ARUP

Acceptable test for detecting Chlamydia trachomatis in certain sample types only. Preferred test is one of the combined nucleic acid amplification tests (NAAT) (ie, Chlamydia trachomatis & Neisseria gonorrhoeae by Amplified Detection [APTIMA®] 0060241 ). Laboratory Collection Information The collection containers for testing chlamydia on ocular samples have changed. Beginning immediately, use the APTIMA Unisex Swab Specimen Collection kit. This is the same kit that is currently used for GC/ Chlamydia endocervical and urethra collections. Samples collected using the PACE collection kit will not be tested. Ocular / conjunctival specimen collection instructions: 1. If pus or discharge is present, use the cleaning swab (white) to clean the

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area. Do not scrape the conjunctiva while cleaning the eye(s). Discard the cleaning swab. 2. If both eyes are affected, swab the least-affected eye first. 3. Thoroughly swab the lower, then the upper conjunctiva two to three times each with the Unisex collection swab (blue) from the APTIMA Unisex Swab Specimen Collection kit. 4. Place the specimen swab (blue swab only) into an appropriately labeled Gen-Probe transport media tube . 5. Snap off the swab shaft at the score line or cut shaft to fit tube. 6. Cap tube. Chlam Tracho, DNA 7. Store and transport at room temperature (15-30°C). Probe, Eye Specimen Preparation : Transport blue swab in Aptima transport media. Storage/Transport Temperature: Refrigerated. Unacceptable Conditions: Gross presence of blood in the specimen. Stability (collection to initiation of testing): Ambient: 1 week Refrigerated: 1 week; Frozen: 1 month. TC# 60243

Transport: Refrigerate if held.

Preferred Collection/Volume: Aptima Collection Kit Only perform collection with blue colored swab.

TAT: 1-4 Days Test Schedule: Run daily, Mon - Fri Method: Nucleic Acid Amplification Test: Transcription Mediated Amplification (TMA). Test Facility: AWL: Molecular Diagnostics

Chlam/GC, Swab Amp Patient Preparation: Male Urethral collection: Patient should not have urinated for at least one Probe hour prior to collection.

See Panel CTO for ocular collection. Rejection Criteria: White swab or no swab in Aptima Transport tube

Transport: Transport at 2 - 30 C. Process within 60 days of collection.

Preferred Collection/Volume: Urine Cup transferred to Aptima Urine Kit Direct patient to collect 20 - 30 mL of first catch urine into a sterile, leakproof container.

TAT: 1-4 Days Test Schedule: Run daily, Mon - Fri Chlam/GC, Urine Amp Method: Nucleic Acid Amplification Test: Transcription Mediated Amplification (TMA). Probe Test Facility: AWL: Molecular Diagnostics

Patient Preparation: Patient should not have urinated for at least one hour prior to collection.

Rejection Criteria: Mid-stream urine Over, or under filled Aptima Tranport tube

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Processing: Transfer 2 mL of urine into Aptima transport tube using provided pipette so that urine level is between the black lines on the tube. Transfer must be done within 24 hours of collection. Transport: Transport at 2 - 30 C. Process within 30 days of collection.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Saturday Morning Method: Micro Immunofluorescence Assay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Chlamydia Antibody Clinical Data: Chlamydia/Chlamydophila species include C. pneumoniae, C. psittaci, and C. trachomatis. Each may cause pneumonia and other overlapping medical conditions.

Specimen Stability: Room Temperature: 7 days Refrigerated: 14 days Frozen: 30 days Test Code: CHANTILLY TC 143312

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 1 -3 days Test Schedule: Mon-Sat a.m Method: Indirect Immunofluorescence Assay Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave. Cypress, CA 90630

Clinical Data: This test uses Chlamydial initial body inclusions from the L2 serotype of C. Chlamydia Antibody trachomatis in cultured cell monolayers. The inclusions are broadly reactive to both Chlamydial IgG genus and species specific antibody. Thus this test is employed as a screen to detect antibody produced against all chlamydial species. Since exposure in humans to chlamydia is common, this test is of limited value for the differential diagnosis of infections caused by the different chlamydial species. See Chlamydia Species Differentiation Antibody Panel, IFA (Focus Unit Code 23000).

Specimen Stability: Room temperature: 5 days Refrigerated: 14 days Frozen: 30 days (glass tubes unacceptable) Test Code: QUEST TC 20172

Processing: Centrifuge and aliquot serum into referred test aliquot tube. Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: Collection Instructions: Proper specimen collection from the patient is extremely critical for successful isolation and identification of infectious organisms. For specific guidance regarding specimen collection procedures, follow industry standards for collecting infectious organisms. Specimens should be collected as soon as possible after clinical onset of disease. Highest Chlamydia titers are present during the acute illness.

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FOR VCM MEDIUM VIALS: 1. Aseptically remove cap from vial. 2. Aseptically place sample into the vial with medium. 3. Replace cap on vial and close tightly. 4. Label with appropriate patient information. 5. Send to the laboratory for immediate analysis. M4 Transport Medium is also acceptable. Body Fluid in VCM Volume: 3.0 mL -or- Swab VCM -or- VCM Tissue Alternative Collection: Swab M4 Transport Media -OR- Tissue M4 Transport Media -OR- 3.0 mL Body Fluid M4 Transport Media

Chlamydia Culture. TAT: Report available: 3 Days Test Schedule: Monday-Saturday Morning Method: Culture Indirect Immunofluorescence Assay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: C. trachomatis is associated with infections of the mucous membranes of the urogenital system, the upper respiratory tract and the eye. It may be sexually transmitted; resulting diseases include urethritis, cervicitis, salpingitis, epididymitis, proctitis, and lymphogranuloma venereum. Coinfection with C. trachomatis and N. gonorrhoeae can occur. Infections of the upper respiratory tract and eye usually occur in newborns exposed at parturition. In adults, eye infection may be transmitted by hand after contact with secretions. Isolation by tissue culture is recommended when testing individuals under the age of 13 years.

Specimen Stability: Room Temperature: Unacceptable Refrigerated: 48 hours Frozen: 30 days (-70c) Test Code: CHANTILLY TC 877

Processing: Freeze solid Transport: Transport frozen on ice

Preferred Collection/Volume: RED 10 or RED SST Volume: 5 mL Serum Alternative Collection: LAV EDTA Volume: 5 mL plasma

TAT: 6 days Test Schedule: Tues, Thurs a.m Chloral Hydrate Level Method: Gas Chromatography Test Facility: National Medical Services 3701 Welsh Rd Willow Grove, PA 19090

specimen stability: Room temperature: 7 days Refrigerated: 14 days Frozen: 14 months Test Code: Quest TC 4201

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Processing: Centrifuge and aliquot serum into Referred Tests aliquot tube. Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5 mL p Alternative Collection: RED PST or serum acceptable Volume: 0.2 mL s

TAT: 1 HR / 24 HR Test Schedule: Daily Chloride Level Method: ISE Test Facility: SMC, INT, AWL, BVT, CAS, LVK, SKY, NLR, RKW, SAL, VAN, DIV, SST, TUA, ORC

iSTAT Testing. Whole blood from unspun GNS5 acceptable for testing.

Processing: Centrifuge. Refrigerate if held.

Preferred Collection/Volume: 24 hr urine (no preservative)/4.0mL u Alternative Collection: 12x75 aliquot/1.0mL u

TAT: 24 HR Test Schedule: Daily Method: ISE Chloride Level 24 Hour Test Facility: AWL Urine Refrigerate during collection. Other Acceptable Preserv: Boric acid (10g). See panel Chloride Level Urine for random specimen

Processing: Record collection interval hrs. Measure and record total vol. in LIS, immediate result field. Aliquot to 12x75 tube. Refrigerate.

Preferred Collection/Volume: AP99 with 12x75 aliquot/4.0mL u Alternative Collection: 1.0mL u

TAT: 24 HR Test Schedule: Daily Chloride Level Urine Method: ISE Test Facility: AWL

Processing: Aliquot to 12x75 tube. Refrigerate.

Preferred Collection/Volume: RED 10 Volume: 3.0 mL Serum Alternative Collection: LAV EDTA Volume:3.0 mL Plasma

TAT: 3 days Chlorpromazine Level Test Schedule: Monday-Friday Method: Gas Chromatography (GC) Test Facility: NMS LABS 3701 Welsh Road Willow Grove, PA 19090

Clinical Data: Chlorpromazine is a phenothiazine for treating psychosis and Tourettes syndrome. Monitoring its level is important to optimize therapy, to avoid toxicity, and to assure

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compliance.

NOTE: Test performed at NMS via Quest Chantilly. Specimen Stability: Room Temperature: 7 day(s) Refrigerated: 14 day(s) Frozen (-20 °C): 12 month(s) Test Code: QUEST TC 581

Processing: Centrifuge and aliquot into a Referred Test aliquot tube.Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: LIGREEN4/4.0mL bf Alternative Collection: RD10/1.0mL bf

TAT: 24 HR Test Schedule: Daily Cholesterol Body Fluid Method: Enzymatic Colorimetric Test Facility: AWL

Use separate LIS requests if both blood and body fluids are ordered.

Processing: Refrigerate if held.

Preferred Collection/Volume: GS5/0.5mL p/s Alternative Collection: SSRED5, LV5/0.2mL p/s

TAT: 24 HR Cholesterol Direct Low Test Schedule: Daily Density Lipids Method: Enzymatic Colorimetric Test Facility: AWL

Processing: Cent, Aliquot, and Refrigerate if held.

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p Alternative Collection: RED SST or serum acceptable LAV EDTA Absolute Minimum Volume: 0.2mL p/s

TAT: 24 HR Cholesterol HDL Test Schedule: Daily Method: Enzymatic Colorimetric Test Facility: AWL

Fasting is not required.

Processing: Cent. Refrigerate if held.

Preferred Collection/Volume: GRN Li Hep PST Volume 0.5mL p Alternative Collection: RED SST or serum acceptable Volume: 0.2mL s Cholesterol Total TAT: 24 HR Test Schedule: Daily Method: Enzymatic Colorimetric Test Facility: AWL

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Processing: Cent. Refrigerate if held

Preferred Collection/Volume: RED 10 Alternative Collection : RED SST Volume: 1.0 mL s

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning Method: Kinetic Spectrophotometric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: The Pseudocholinesterase phenotype can be determined by analysis of Cholinesterase, Pseudocholinesterase and the percent inhibition by Dibucaine. Approximately 96% of the Dibucaine Inhibition population have normal activity, approximately 4% will exhibit decreased activity that leads to prolonged paralysis, following use of succinylcholine, and 1 in 3000 patients will exhibit severe, prolonged paralysis following anesthetic exposure.

Specimen Stability: Room Temperature: 21 days Refrigerated: 21 days Frozen: 30 days TC# 7961

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Transport to AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: Dark Blue EDTA after discard of a first Dark Blue EDTA Volume: 2.0 mL p Minimum Volume: 1.0mL p

TAT: 5 Days Test Schedule: Tuesday, Thursday, Saturday Morning Method: Atomic Absorption Spectrometry with Zeeman Background Correction Testing Location: Quest Diagnostics Nichols Institute 27027 Tourney Road Valencia, CA 91355-5386

Clinical Data: Occupational exposure and exposure to environmental contamination of Chromium may lead to toxicity. The need for Chromium supplements is unproven. Supplements Chromium Level taken in excess may also lead to Chromium toxicity.

Clinician Instructions Only: Patient should refrain from taking vitamins, mineral or herbal supplements at least one week prior to specimen collection

Specimen Stability: Room temperature: 5 days Refrigerated: 10 days Frozen: 21 days TC# 3484X

Processing: Centrifuge and aliquot into acid washed aliquot tube. Do not use filter to aliquot. Pour plasma into acid washed aliquot tube. Transport: To AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: Preferred Specimen Random Urine in Acid Washed Container Volume 2.0 mL Urine Collect and transport in an acid-washed, metal-free, leakproof container.

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TAT: Report available: 7 Days Test Schedule: Tuesday, Thursday Afternoon Method: Atomic Spectroscopy Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Chromium Level Urine Specimen Stability: Room Temperature: 4 days Refrigerated: 14 days Frozen: 1 month Test Code: CHANTILLY TC 13787

Transport: Rerfrigerate if held

Preferred Collection/Volume: RED10 Volume: 1.0mL s Alternative Collection: SSRED Volume: 0.5mL s

TAT: Reports in 3 to 4 days. Test Schedule: Sets up 5 days a week. Method: Electrochemiluminescence Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: is a 49 kDa acidic protein that consists of 439 amino acids encoded on chromosome 14. Chromogranin A has been identified in a number of normal and neoplastic endocrine tissues. It was demonstrated that an elevated circulating chromogranin A level would be a marker of tumors of neuroendocrine origin. However, the most significant clinical use of Chromogranin A chromogranin A is related to the diagnostic procedure in patients of pheochromocytoma. Chromogranin A is a very sensitive (83%) and highly specific (96%) marker in the evaluation of actual or suspected pheochromocytoma. Drugs commonly employed in the diagnosis or treatment of pheochromocytoma

Specimen Stability: Room temperature: 7 Days Refrigerated: 7 Days Frozen : 5 Weeks TC# 16379 Reject Criteria Gross hemolysis • Gross lipemia • Gross icteria

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: Submit in sterile centrifuge tube. Preferred Volume: 20-25cc

TAT: Average 8-11 days for preliminary result Test Schedule: Mon-Fri Chromosome Analysis Method: Tissue culture; microscopic analysis Amniotic Fluid Test Facility: AWL

Clinical Data: Include clinical indication Patient History: n/a

Patient Preparation: n/a Labeling: n/a

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Processing: Do not Centrifuge. Hold at room temperature. Notify Cytogenetics at 503-258-6723 (Tie Line: 35-6723) before sending specimen. Transport: Room Temperature.

Preferred Collection/Volume: CVS Transport Media/ 20mg Minimum Volume: 5 mg Transport media provided by Cytogenetics Alternative Collection: n/a

TAT: Average 10-14 days for preliminary result Test Schedule: Mon-Fri Method: Tissue culture; microscopic analysis Test Facility: AWL Chromosome Analysis Chorionic Villus Sam Clinical Data: Include clinical indication Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: Hold at Room temperature. Notify Cytogenetic s at 503-258-6723 (Tie Line: 35-6723) before sending specimen. Transport: Room Temperature.

Preferred Collection/Volume: Bone Marrow Transport Media/1cc. Transport media provided by Cytogenetics. Alternative Collection: Green 3 Sodium Heparin

TAT: Average 2-5 days for preliminary result Test Schedule: Mon-Fri Method: Cell culture; microscopic analysis Chromosome Analysis, Test Facility: AWL Cell Culture, Bone Marrow Clinical Data: Include clinical indication Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: Do not Centrifuge. Hold at Room temperature. Transport: Room Temperature

Preferred Collection/Volume: ***NO GEL BARRIER** Green Sodium Heparin Volume: 3mL Whole Blood

TAT: Routine: 14 - 21 Days STAT: 48 - 72 Hours Test Schedule: Mon-Fri Method: Cell culture; microscopic analysis Chromosome Analysis, Test Facility: AWL Lymphocyte Culture, Blood Clinical Data: Include clinical indication. If ordered in combination with FISH (ANF, VCF, WIF, SMF, PWF) only 1 specimen is needed Patient History: n/a

Patient Preparation: n/a Labeling: n/a

** For newborns 1mL is preferred but any specimen volume will be accepted ** Do not reject a sample of any volume.

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Processing: DO NOT CENTRIFUGE Hold at Room temperature. Do NOT reject centrifuged specimen; contact department at 503-258-6723 (Tie Line: 35-6723) Transport: Room Temperature

Preferred Collection/Volume: Tissue Biopsy Transport Media / 1 cm sq. Transport media provided by Cytogenetics Alternative Collection: Sterile saline or RPMI Media

TAT: Varies by Specimen type Test Schedule: Mon-Fri Method: Tissue culture; microscopic analysis Test Facility: AWL Chromosome Analysis, Solid Tumor Clinical Data: Include clinical indication. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: Hold at room temperature Transport: Room Temperature

Preferred Collection/Volume: Tissue Biopsy Transport Media / 3mm sq. Transport media provided by Cytogenetics. Alternative Collection: Sterile saline in sterile container

TAT: 3-6 Weeks Test Schedule: Mon-Fri Method: Tissue culture; microscopic analysis Test Facility: AWL Chromosome Analysis, Tissue Clinical Data: Include clinical indication. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: ** No formalin - Fresh tissue only ** Hold at room temperature Transport: Room Temperature

Preferred Collection Grn NA HEP (patient) AND Grn NA HEP (contro/KP Employee). Alternative Collection: Grn Li HEP (patient) AND Grn Li HEP (contro/KP Employee). Volume: 3mL wb (patient) AND 3 mL wb (contol=KP Employee) Note: Draw specimens Monday-Thursday only. Patient and control specimens must be collected within 48 hours of test performance. Collect control specimen from a healthy KP Employee at approximately the same time as and under similar conditions to the patient.

TAT: 2-3 days Test Schedule: Draw Mon-Thur ONLY Method: Semi-Quantitative Flow Cytometry Testing Location: ARUP Laboratories

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500 Chipeta Way Salt Lake City, UT 84108-1221

Clinical Data: Chronic granulomatous disease (CGD) is a leukocyte function defect where phagocytic cells ingest but do not digest bacteria due to a malfunction of a nicotinamide adenosine dinucleotide phosphate (NADPH) oxidase system. CGD is characterized by severe, recurring infections with granuloma formation. White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by flow cytometry.

Labeling: Label the control specimen as "control" and indicate the sex of control/KP employee Chronic Granulomatous Disease, DHR Oxidation Specimen Stability: (collection to initiation of testing): Room Temperature: 48 hours Refrigerated: Unacceptable Frozen: Unacceptable TC# 0096657

Processing: Transport 3 mL wb (patient) AND 3 mLwb (control=KP Employee) in original collection tubes. Transport: Room Temperature Note: Do not refrigerate or freeze specimens, LIVE NEUTROPHILS REQUIRED.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL serum (x3)

TAT: Report available: Next Day Test Schedule: Tues. Thurs., a.m Method: Enzyme Immunoassay • Immunoturbidimetric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Patient Preparation: Includes C4 and C3 Complement Group, C3d Circulating Immune Circulating Immune Complexes, Immune Comples Detection by C1q Binding Complex Transport the 3 specimens together. Specimen Stability: Room temperature: unacceptable Refrigerated: unacceptable Frozen: 7 days Do not thaw. Test Code: CHANTILLY TC 12836

Processing: Centrifuge and transfer 1 mL of serum into 3 separate Referred Tests aliquot tubes. Freeze solid. Do not thaw. Transport: Transport 3 frozen specimens together on ice

Preferred Collection/Volume: 24-hour urine collected without preservative Acidified urine unacceptable Volume: 10.0 mL u

TAT: Next Day Test Schedule: Monday-Friday Evening Method: Enzymatic Spectrophotometric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

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Clinical Data: Citrate binds to calcium and inhibits kidney stone formation. Thus, low concentrations of citrate may lead to kidney stone formation. This is the most important risk factor for kidney stone formation in children. The treatment of calcium stones involves increasing urinary citrate excretion.

Citrate Urine, 24 Hours with Creatinine

Reject Criteria: Citrate Level 24 Hour Acidified urine Urine Specimen Stability: Room Temperature: 8 hours Refrigerated: 30 days Frozen: 90 days TC# 14464

Processing: Mix sample well and aliquot. Note on sample total volume and preservative used during collection. Transport: Transport to Airport Way Regional Laboratory

Preferred Collection/Volume: GRN Li HepPST Volume: 0.5 mL p Alternative Collection: SSRED5, Serum, LAV EDTA Volume: 0.5 mL s

TAT: Same Troponin Test Schedule: Daily Method: Immunoassay CK-MB Isoenzyme Test Facility: Sunnyside Medical Center Westside Medical Center

Add On Testing Stability: 5 days if refrigerated @ 2-8 degrees celsius

Processing: Centrifuge Aliquot sample if serum Transportation: Transport to testing location on ice

Preferred Collection/Volume: Dark Blue Serum Volume: 2.0 mL Serum Alternative Collection: RED 10, No gel barrier Volume: 2.0 mL Serum

TAT: Report available: 3 Days Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute Clomipramine Level 14225 Newbrook Drive and Metabolite Chantilly, VA 20153 Specimen Stability: Room Temperature: 3 Days Refrigerated: 3 Days Frozen: 4 Months (-20c) Test Code: CHANTILLY TC 2040

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

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Alternative Collection: LAV EDTA Volume 2.0 mL Plasma

TAT: Report available: 3 Days Test Schedule: Monday, Wednesday, Friday Afternoon Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Clonazepam is a benzodiazepine used as a tranquilizer. Clonazepam is used in Clonazepam Level treating patients with seizures and in reducing tardive dyskinesia. Therapeutic drug monitoring is useful to avoid toxicity.

Specimen Stability: Room Temperature: 24 hours Refrigerated: 48 hours Frozen: 14 days Test Code: CHANTILLY TC 7052

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Note if serum or plasma on specimen. Freeze. Transport: Transport frozen on ice

Preferred Collection/Volume: RED 10 Volume: 2mL Serum Alternative Collection: LAV EDTA Volume: 2.0 mL Plasma

TAT: 8 days Test Schedule: Friday Method: Liquid Chromatography, Tandem Mass Spectrometry (LC/MS/MS) Test Facility: Quest Diagnostics Nichols Institute 27027 Tourney Road Valencia, CA 91355-5386

Clinical Data: Orally administered clorazepate is rapidly decarboxylated to its metabolite, Clorazepate Level nordiazepam. There is essentially no circulating patient drug. Nordiazepam elimination half-life is about 40 to 50 hours. Plasma levels of nordiazepam increase proportionally with clorazepate does and show moderate accumulation with repeated administration.

Specimen Stability: Room temperature: Unacceptable Refrigerated: 7 days Frozen: 60 days Test Code: Quest TC 90854

Processing: Centrifuge and aliquot serum/plasma into a referred tests aliquot tube. Note if serum or plasma on specimen. Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: Denture cup

TAT: 24 HR Routine Test Schedule: Daily Method: Immunoassay Test Facility: Sunnyside and Westside Medical Centers Airport Way Regional Laboratory

Submit a single, freshly passed, liquid fecal specimen. Clostridium Difficile The laboratory will not perform additional testing on a patient known to have had a Antigen and Toxin A positive test within the previous 14 days.

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and B w Reflex to PCR This test is offerred 24 hours per day and 7 days per week at the Medical Centers and Airport Way Regional Laboratory.

Assay detects C. difficile GDH antigen and Toxins A and B.

Processing: Keep specimen cold and transport to the laboratory as soon as possible. Can be stored up to 3 days refrigerated. Specimens can be frozen if transportation will be delayed greater than 72 hours.

Preferred Collection/Volume: LAV EDTA Volume: 2.0mL p

TAT: Report available: Next day Test Schedule: Set up: Monday-Friday Morning Method: High Performance Liquid Chromatography (HPLC) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Clozapine is used selectively in the treatment of patients with schizophrenia. The Clozapine Level major active metabolite is norclozapine. Hematologic parameters may be affected. Therapeutic drug monitoring is useful to optimize dose and to avoid toxicity.

Specimen Stability: Room temperature: 24 hours Refrigerated: 5 days Frozen: 30 days TC# 1769

Processing: Centrifuge and immediately separate plasma into referred tests aliquot tube. Freeze solid. Transport: Transport frozen on ice

Preferred Collection/Volume: Collect Mon-Thurs AM Only. Specimen must reach AWL by 1300. 4 LAV EDTA (Whole Blood) Volume: 24.0 mL Whole Blood

TAT: 21-28 days Test Schedule: Mon-Thurs AM ONLY Method: Multiplex Ligation-dependent Probe Amplification, Southern blot, Polymerase Chain Reaction (PCR) and DNA Sequencing Test Facility: Athena Diagnostics Four Biotech Park, 377 Plantation St Worchester, MA 01605 CMT Profile, Dominant Evaluation Clinical Data: Peripheral Neuropathy Disease tested for: Charcot-Marie-Tooth disease (CMT) Detects duplications/deletions in the PMP22 gene, and sequence variations in PMP22, MPZ (P0), EGR2, CX32 (GJB1), NFL, LITAF/SIMPLE, MFN2, GARS, RAB7, and HSPB1 genes

Patient Preparation: Drawn Monday-Thursday morning only. Specimen must reach AWL by 1300 same day.

Specimen Stability: Room Temperature: Must Ship same day Refrigerated: Unacceptable Frozen: Unacceptable. Test Code: ATHENA TC 414

Processing: Do Not Centrifuge. Transport: Send Room temperature, Transport Immediately.

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Preferred Collection/Volume: Collection Instructions: Collect Mon-Thurs AM Only. Specimen must reach AWL by 1300. LAV EDTA (Whole Blood) Volume: 12.0 mL Whole Blood

TAT: 21 days Test Schedule: Mon-Thurs ONLY Method: Polymerase Chain Reaction (PCR) and DNA sequencing Test Facility: Athena Diagnostics Four Biotech Park, 377 Plantation St Worchester, MA 01605

Clinical Data: Peripheral NeuropathyDisease tested for: Charcot-Marie-Tooth disease, Type 1B CMT1a MPZ Mutation (CMT1B), CMT2I (CMT Type 2I), CMT2J (CMT Type 2J). CMT with a family history of MPZ Analysis mutations identified in a proband. Detects sequence variations in the Myelin Protein Zero gene.

Patient Preparation: Drawn Monday-Thursday morning only. Specimen must reach AWL by 1300 same day.

Specimen Stability: Room Temperature: Must Ship same day Refrigerated: Unacceptable Frozen: Unacceptable. Test Code: ATHENA TC 134

Processing: Do Not Centrifuge. Transport: Send Room temperature, Transport Immediately.

Preferred Collection/Volume: Collect Mon-Thurs AM Only. Specimen must reach AWL by 1300. 4 LAV EDTA (Whole Blood) Volume: 24.0 mL Whole Blood

TAT: 14-21 days Test Schedule: Mon-Thurs ONLY Method: Multiplex Ligation-dependent Probe Amplification Test Facility: Athena Diagnostics Four Biotech Park, 377 Plantation St Worchester, MA 01605 CMT1a PMP22 Clinical Data: Peripheral Neuropathy Disease tested for: Charcot-Marie-Tooth disease, Type Mutation Analysis 1A (CMT1A) CMT with a family history of a PMP22 duplication or a deletion identified in a proband. Detects rearrangements in the PMP22 gene. This test performs both duplication and deletion analysis to detect the cause of CMT1A and HNPP.

Patient Preparation: Drawn Monday-Thursday morning only. Specimen must reach AWL by 1300 same day.

Test Code: ATHENA TC 131

Processing: Do Not Centrifuge. Transport: Send Room temperature. Transport Immediately.

Preferred Collection/Volume: Collect Mon-Thurs AM Only. Specimen must reach AWL by 1300. LAV EDTA (Whole Blood) Volume: 12.0 mL Whole Blood

TAT: 21 days Test Schedule: Mon-Thurs ONLY Method: Multiplex Ligation-dependent Probe Amplification

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Test Facility: Athena Diagnostics Four Biotech Park, 377 Plantation St Worchester, MA 01605

Clinical Data: Peripheral Neuropathy Disease tested for: Charcot-Marie-Tooth disease, Type 1X (CMT1X/CMTX) CMT with a family history of Connexin32 mutations identified in a proband. Detects sequence variations in Connexin32 (CX32, GJB1) gene.

Patient Preparation: Drawn Monday-Thursday morning only. Specimen must reach AWL by 1300 CMTx1 XQ13.1 same day. Mutation Analysis Specimen Stability: Room Temperature: Must Ship same day Refrigerated: Unacceptable Frozen: Unacceptable. Test Code: ATHENA TC 143

Processing: Do Not Centrifuge. Transport: Room temperature.Transport Immediately.

Preferred Collection/Volume: SSRED5/1.0 mL s Alternative Collection: RD10/1.0 mL s

TAT: 24 HR Test Schedule: M-F CMV Antibody IgG Method: Multiplex flow Immunoassay Test Facility: AWL

Notes: This test for immune purposes

Processing: Centrifuge, aliquot if RD10. Refrigerate if held.

Preferred Collection/Volume: Plasma is unacceptable for this test. Rejection criterion: Grossly hemolyzed or lipemic samples are unacceptable RED 10 Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning Method: Enzyme Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 CMV Antibody IgM Specimen Stability : Room Temperature: 4 days Refrigerated: 7 days Frozen: 30 days Plasma is unacceptable for this test. Rejection criterion: Grossly hemolyzed or lipemic samples are unacceptable Test Code: CHANTILLY TC 8503

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held

Preferred Collection: Whole blood collected in a EDTA or ACD tube Amniotic, CSF, or random urine submitted in a sterile leak-proof container Volume: 1mL

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Minimum Volume: 0.3 mL Alternative Collection: Bronchial brush/washing/lavage or eye fluid submitted in a sterile leak-proof container Volume: 1 mL Minimum Volume: 0.3 mL Fresh or frozen tissue (not in fixative) in sterile container Volume: 3 mm

TAT: Report is available 1 day from receipt of specimen at testing location. Testing Schedule: Tests are sent out M-F Testing Method: Real-Time Polymerase Chain Reaction (RT-PCR) Testing Location: Quest Diagnostics Nichols Institute CMV DNA Qual by PCR 14225 Newbrook Drive Chantilly, VA 20153 Test Code: 10601 Specimen Stability: Room temperature: 48 hours Refrigerated: 8 days Frozen: 30 days (Do not freeze whole blood. Store refrigerated.) Note: AWl Freeze specimens (except whole blood). Whole blood - store refrigerated. Rejection Criteria: Blood collected in heparin tubes. Frozen whole blood.

Processing: Refrigerate Specimen Transportation: Send specimen on ice to Airport Way Lab Referred Test Department.

Preferred Collection: 2 LAV EDTA Tubes Volume: 3.0mL (aliquoted plasma)

TAT: 1-4 days CMV DNA Quant by Testing Method: Real-Time PCR PCR Testing Location: Airport Way Lab Molecular Department Specimen Stability Refrigerated: 7 days if centrifuged and aliquoted within 24 hours of collection. Frozen: 42 days if centrifuged and aliquoted within 24 hours of collection. Specimen Rejection:

{ Frozen whole blood

{ Whole blood stored 2-25 c for >6 hours

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{ Specimens collected in a RED 10, Li Hep, DRK Blue EDTA

Note: This is a quantitative molecular test, with a linear range of 137-9,100,000 IU/mL.

Processing: Centrifuge specimen within 6 hours of collection at 800 to 1600xg for 20 minutes, then aliquot into a referred test aliquot container. Note: Must ruber band both aliquot containers together. Transportation: Transport aliquoted specimens together on ice to Airport Way Lab.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 5.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning Method: CMV: Enzyme Immunoassay Rubella: Immunoassay Herpes: Enzyme-Linked Immunosorbent Assay (ELISA) CMV, Rubella, Herpes Test Facility: Quest Diagnostics Nichols Institute (Torch) 14225 Newbrook Drive Chantilly, VA 20153

Specimen Stability: Room Temperature: 4 days Refrigerated: 7 days Frozen: 30 days Gross hemolysis and lipemia are cause for specimen rejection. Test Code: CHANTILLY TC 12752

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held

Preferred Collection/Volume: Dark Blue EDTA Volume 7.0 mL Whole Blood.

TAT: Report available: 2 Days Test Schedule: Monday-Friday Evening Method: Inductively Coupled Plasma-Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Cobalt Level Clinical Data: Cobalt is part of our diet. Approximately 85% of absorbed cobalt is excreted in the urine and the remainder is eliminated in stool. Toxicity may occur in select industrial environments. Cobalt is not mined in the United States, so primary supplies are imported.

Patient Preparation: Patient should refrain from taking mineral supplements, vitamin B12, or vitamin B complex three days before specimen collection.(Refer Patient to Clinician to determine if safe to refrain from dietary supplements)

Specimen Stability: Room Temperature: 48 hours Refrigerated: 5 days Frozen: unacceptable

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Test Code: CHANTILLY TC 35417

Processing: Do not centirfuge. Transport: Refrigerate if held

Preferred Collection/Volume: AP99 Random Urine/50 mL u Alternative Collection: 10 mL u

TAT: 48 Hours Test Schedule: M, W, F Metabolite Method: GC/MS Urine Confirmatory Test Facility: AWL

To order a drug screen see panels Cocaine Screen Urine, Drug Screen Abbreviated, Drug Screen Basic or Drug Screen Comprehensive.

Processing: Refrigerate if held overnight.

Preferred Collection/Volume: AP99 Random Urine/50 mL u Alternative Collection: 10 mL u

TAT: 24 hours Test Schedule: Daily Method: Immunoassay Cocaine Screen Urine Test Facility: AWL

Included in drug screen panels Drug Screen Abbreviated, Drug Screen Basic or Drug Screen Comprehensive.

Processing: Refrigerate if held overnight.

Preferred Collection/Volume: Sterile container Volume: 3.0mL CSF Coccidiomycosis Ab, Gel Diffusion w Reflex Specimen Stability: To Complement Refrigerated Only Fixation, CSF Transport: Transport on ice.

Preferred Collection/Volume: RED 10 Volume: 5.0 mL serum

Test Facility: UC DAVIS Coccidioidomycosis Serology Laboratory 3146 Tupper Hall 1 Shields Avenue Coccido Ab Ser Davis, CA 95616

Specimen Stability: Refrigerated Only

Processing: Centrifue and aliquot serum into Referred tests aliquot tube Transport: Transport on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 3.0 mL Serum

TAT: Report available: 2 Days

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Test Schedule: Monday-Saturday Afternoon Method: Hemagglutination Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: This test can be useful for the detection of cold agglutinins in association with cold agglutinin syndrome. If testing is for recent Mycoplasma pneumoniae exposure, Test Code 4788, M. pneumoniae IgM Antibodies, EIA, is recommended.

Cold Agglutinins Specimen Stability: Room Temperature: 14 days Refrigerated: 28 days Frozen: unacceptable Test Code: CHANTILLY TC 0142

Processing: SPECIAL HANDLING IS CRITICAL Clot at 37 degrees C. Centrifuge at room temperature and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 or RED SST Volume: 1.0 mL serum

TAT: 3 days Test Schedule: Mon-Fri morning Method: Immunofluorescence Assay (IFA) Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave, Suite 200 Cypress, CA 90630

Clinical Data: Colorado tick fever is an infection transmitted by the bite of the wood tick Dermacentor andersoni. The disease occurs almost exclusively in the western United States and Colorado Tick Fever Ab Canada and is caused by an RNA virus (genus Coltivirus, Family Reoviridae). Patients with Colorado Tick Fever demonstrate a seroconversion or four-fold rise in IFA titer between acute and convalescent sera. Also, the detection of IgM indicates acute infection.

Specimen Stability: Room temperature: 7 days Refrigerated: 14 days Frozen: 30 days Test Code: Quest TC 111432

Processing: Centrifuge and aliquot into a Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma -

TAT: Report available: 5 Days Complement C7 Test Schedule: Monday, Wednesday Night Method: Radial Immunodiffusion Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: The complement system is critical to inflammatory responses. C5-C9 are components of the common pathway. Patients with congenital deficiency of any of these components are at increased risk of meningococcal meningitis. Unlike the other components, C7 is

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synthesized in the liver, hematopoietic cells, fibroblasts, and microglial cells, and astrocytes of the central nervous system.

Specimen Stability: Room Temperature: 48 hours Refrigerated: 2 weeks Frozen: 1 month Test Code: CHANTILLY TC 66932 TO SJC TC 34888X

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Note if serum or plasma on specimen. Transport: Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: 5 days Test Schedule: Set up: 1 day a week Method: Radial Immunodiffusion (RID) Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Complement C8 Clinical Data: The complement system is critical to inflammatory responses. C5-C9 are components of the common pathway. Patients with congenital deficiency of any of these components are at increased risk of meningococcal meningitis.

Specimen Stability: Room temperature: 7 days Refrigerated: 14 days Frozen: 21 days Test Code: SJC TC 34889X

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Note if serum or plasma on specimen. Transport: Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: 4 days Test Schedule: Set up: 2 days a week Method: Radial Immunodiffusion (RID) Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway Complement C9 San Juan Capistrano, CA 92690-6130

Clinical Data: The complement system is critical to inflammatory responses. C5-C9 are components of the common pathway. Patients with congenital deficiency of any of these components are at increased risk of meningococcal meningitis.

Specimen Stability: Room temperature: 48 hours Refrigerated: 14 days Frozen: 30 days Test Code: SJC TC 34896

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Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Note if serum or plasma on specimen. Transport: Refrigerate if held

Preferred Collection/Volume: Avoid hemolysis RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum. CSF, Pleural Fluid, Pericardial Fluid, and Synovial Fluid are unacceptable

TAT: Report available: Next Day Test Schedule: Monday-Saturday Morning Method: Colorimetric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Total Complement (CH50) concentration is an overall measure of the complement system. Decreased concentrations are observed in patients with complement factor deficiencies and disease activity, e.g., systemic lupus erythematosus (SLE) and immune complex Complement Total disease.

Patient Preparation: CSF, Pleural Fluid, Pericardial Fluid, and Synovial Fluid are unacceptable for this test.

Send samples frozen on dry ice. Specimen Stability: Room Temperature: unacceptable Refrigerated: unacceptable Frozen: 30 days Do not allow samples to thaw. Test Code: CHANTILLY TC 618

Processing: Centrifuge specimen within 1 hour of collection and aliquot into Referred Tests aliquot tube. Freeze. Transport: Transport frozen on ice

Preferred Collection: GRN Micro Li Hep w/sep. Minimum: One full tube (filled to line closest to cap). Preferred: Two tubes Alternative Collection: Amber Micro w/sep Volume: 0.6mL plasma (Absolute minimum volume: 0.25mL plasma)

TAT: STAT 1 Hour Routine 24 Hours Test Schedule: Daily Comprehensive Metabolic Neonatal Method: Photometric, Diazonium Salt (DPD) in acid Panel Testing Location: Sunnyside Medical Center and Westside Medical Center

Intended for neonates and infants, generally used up to 30 days of age but continue to order if bilirubin is monitored beyond 30 days of age. Note: TEST NOT PERFORMED AT AWL

Processing: Centrifuge. Protect specimen collected in GRN Li Hep from light. Refrigerate.

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Preferred Collection GRN Li Hep PST Volume: 2.0 mL Plasma Alternative Collection: RED SST Minimum Volume : 1.0 mL Plasma or Serum Comprehensive Metabolic Panel TAT: 1 - 24 hours Testing Schedule: Daily Note: Analytes include NA,K,Cl,CO2, Non-fasting GLU, BUN,Creat, T bili, T protein, Alb, AST, ALT, Alk phos and Calcium. Direct Bilirubin will be assayed as a reflex test if Total Bilirubin is greater than 1.1 mg/dL and patient is older than 30 days .

Processing: Centrifuge. DO NOT REMOVE CAP. Refrigerate.

Preferred Collection GRN Li Hep PST Volume: 2.0 mL Plasma Alternative Collection: RED SST Minimum Volume : 1.0 mL Plasma or Serum Comprehensive Metabolic Panel, TAT: 1 - 24 hours Fasting Testing Schedule: Daily Note: Analytes include NA,K,Cl,CO2, Non-fasting GLU, BUN,Creat, T bili, T protein, Alb, AST, ALT, Alk phos and Calcium. Direct Bilirubin will be assayed as a reflex test if Total Bilirubin is greater than 1.1 mg/dL and patient is older than 30 days .

Processing: Centrifuge. DO NOT REMOVE CAP. Refrigerate.

Preferred Collection/Volume: Brucella:RED 10 Volume: 2 mL Serum Leptospirosis:RED 10 Volume: 2 mL Serum Tularemia: RED 10 Volume: 2 mL Serum Rickettsia: RED 10 Volume: 10 mL Serum

Convalescent Serology TAT: Brucella: 1-6 days Infectious Disease Leptospirosis: 1-6 days Tularemia: 1-6 days Ricketsia:2 - 7 working days Test Schedule: Brucella: Weekly Leptospirosis: Weekly Tularemia: Weekly Rickettsia: Weekly Method: Brucella: Bacterial Agglutination Leptospirosis:Indirect Hemagglutination (IHA) Tularemia:Bacterial Agglutination Rickettsia:Indirect Immunofluorescence Assay (IFA)

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Test Facility: Oregon State Lab 3150 NW 229th Ave., Suite 100 Hillsboro, OR 97124-6536

Patient History: Required Information: Date of onset , and list which of the following viral serology tests: Brucella, Leptospirosis, Tularemia, or Rickettsia Panel.

Specimen Stability: Refrigerated only

Processing: Centrifuge and aliquot serum into Referred Tests aliquot tube Transport: Transport on ice.

Preferred Collection/Volume: Dark Blue EDTA Volume: 2.0 mL p

TAT: Report available: Next Day Test Schedule: Monday-Friday Afternoon Method: Atomic Spectroscopy Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Copper Level Clinical Data: Copper is an essential trace element. It is required for hemoglobin synthesis and is a constituent of the cytochrome oxidase system.

Specimen Stability: Room Temperature: 5 Days Refrigerated: 10 Days Frozen: 30 Days Test Code: CHANTILLY TC 363

Processing: Centrifuge and aliquot into acid washed trace metal free aliquot tube. Transport: To AWL in Blue Tote. Refrigerate if held.

Preferred Collection/Volume: 24 hour urine in acid washed container collected without preservatives. Volume 7.0 mL u

TAT: Report available: Next Day Test Schedule: Monday-Friday Afternoon Method: Inductively Coupled Plasma-Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Significance Copper Level 24 Hour Copper is an essential element that is a cofactor of many enzymes. Copper metabolism is Urine disturbed in Wilson's disease, Menkes disease, primary biliary cirrhosis, and Indian childhood cirrhosis. Urinary copper concentrations are also useful to monitor patients on chealation therapy.

Specimen Stability: Room Temperature: 5 days Refrigerated: 5 days Frozen: 2 weeks Test Code: CHANTILLY TC 4876

Processing: Mix sample well and aliquot urine into acid washed urine aliquot container. Note on sample total volume. Transport: Transport to AWL. Refrigerate if held.

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Preferred Collection: Dark Blue Trace tube Volume: 5 mg liver biopsy tissue

Set up Shedule: 1 day a week Methodology: Inductively Coupled Plasma/Mass Spectrometry Testing Location: Quest Diagnostics Nichols Institute Valencia 27027 Tourney Road Valencia, CA 91355-5386 Test Code: Quest San Juan TC#563Z sent to Quest Valencia for testing Copper Level Liver Specimen Stability: Tissue Room Temperature: 28 days Refrigerated: 28 days Frozen: 56 days

Clinical Data: Test is for the evaluation of Wilson's disease.

Processing: 5 mg (minimum: 2 mg) liver biopsy tissue, collected in dark blue serum trace metal-free tube (preferred), shipped at room temperature. Paraffin block is acceptable. Please note that all tissue is consumed and will not be returned to the client. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: 3 Days Test Schedule: 1 Day a week Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Measurement of corticosterone may help confirm the diagnosis of the very rare Corticosterone aldosterone deficiency disorder, corticosterone methyloxidase (18-hydroxylase) enzyme deficiency, where the ratio of corticosterone to 18-hydroxycosticosterone may be increased.

Patient Preparation: Early morning sample is preferred.

Specimen Stability: Room Temperature: 4 weeks Refrigerated: 4 weeks Frozen: 4 weeks Test Code: SJC TC 6547X

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: RED SST Volume: 1.5mL s Alternative Collection: RED 10 Cortisol Volume: (aliquot)/1.0mL s

TAT: 24 Hours Test Schedule: Daily Method: Chemiluminscent Immunoassay

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Test Facility: AWL

Use separate LIS requests for each cortisol if more than one is ordered. See ACTH Stimulation, which includes baseline, 30 min., and 60 min. cortisol. See CRH Stimulation Test.

Processing: Cent. and Refrigerate. Add-on test stability 48 hours.

Preferred Collection: RED SST Alternative Collection: RED 10 Volume: 1.5mL s Minimum Volume: 1.0 mL s

Cortisol AM TAT: 24 hours

Must be collected in the morning before 0900 (9:00AM).

Processing: Centrifuge and Refrigerate

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Reports in 4 days Test Schedule: Tuesday, P.M. Method: Radioimmunoassay Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130 Cortisol Binding Globulin Clinical Data: CBG binds approximately 92% of Cortisol with high affinity. Measurement of CBG is useful in combination with Total and Free Cortisol.

Specimen Stability: Room temperature: 24 Hours Refrigerated: 7 Days Frozen: 2 Years Test Code: CHANTILLY TC 37371 TO SJC TC 37371

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Note if serum or plasma on specimen. Transport: Transport on ice.

Preferred Collection/Volume: Serum Grossly hemolyzed specimens are unacceptable. RED 10 No Gel Barrier Tubes Volume: 2.0 mL Alternative Collection: LAV EDTA Volume: 2.0 mL Plasma

TAT: Reports in 5 days Test Schedule: Sets up 5 days a week, Night Method: Liquid Chromatography Tandem Mass Spectrometry, Equilibrium Dialysis Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway

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San Juan Capistrano, CA 92690-6130

Clinical Data: Free Cortisol is useful in the detection of patients with Cushing's syndrome for whom Free Cortisol concentrations are elevated.

Specimen Stability: Room temperature: 4 Hours Cortisol Free Refrigerated: 7 Days Frozen: 2 Years Test Code: SJC TC 36423X

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Note if serum or plasma on specimen. Transport: Transport on ice.

Preferred Collection/Volume: 24 hr urine collected without preservative Volume: 50mL ur

TAT: 5 Days Test Schedule: Tue, Thur Method: Chemiluminscent Immunoassay Test Facility: AWL Cortisol Free 24 Hour Urine PROCESSING: Record collection interval hrs. Measure and record total vol. Send the AP99 to AWL on ice. Refrigerate if held. AWL PROCESSING: Receive the AP99 in LIS. Reprint a label and aliquot a sample into a 12X75 plastic tube for the Autoline. Send the AP99 to SAM/TOX.

Processing: Mix sample well and aliquot into AP99 container. Note on container total volume and enter TV result into Accession Result Entry in NW PathNet after logging specimen in. Transport : to AWL in blue tote

Preferred Collection/Volume: Saliva submitted in Salivette Volume 0.5 mL

TAT: Reports in 5 days Test Schedule: Sets up 5 days a week, Night Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Patient Preparation: Clinician should designate time of collection for patient. No food or fluid 30 minutes prior to collection. Do not apply creams or lotions that contain steroids or use steriod inhalers immediately Cortisol, Saliva prior to collection. Avoid brushing or flossing teeth prior to collection. Do not collect if gums or inside of mouth is bleeding. If needed, salivation can be stimulated with citric acid crystals or sugar free Kool-Aid powder. Collection Information: Remove the cylindrical shaped swab from the insert and place into mouth. Chew the swab for 30-45 seconds or until swab is saturated and can no longer prevent swallowing excess saliva. If the swab cannot be chewed, place under the tongue for 30-45 seconds until well saturated. Return the swab to the Salivette after it is saturated. Do not remove the tube that is holding the insert. Place stopper firmly on top of the insert for transport to laboratory. Specimen must be labeled with patient name and heatlh record number.

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Not for use with children under the age of three. Use adult supervision for children under the age of ten.

Specimen Stability: Room temperature: 48 Hours Refrigerated: 7 Days Frozen: 2 Years TC# 19897X

Processing: The Salivette(R) should be sent to the lab with the swab inside. Transport: Refrigerate if held.

Preferred Collection/Volume: SSRED5/1.5 mL Alternative Collection: RD10 (aliquot)/0.3 mL

TAT: 24 Hours Test Schedule: Daily Method: Chemiluminscent Immunoassay Test Facility: AWL

Cortrosyn Stimulation ***** ORDER ACS on it's own separate Request. ***** Test must be scheduled w/ Test Nurse Treatment room. Draw baseline cortisol. Patient will go to Nurse Treatment Room for 0.25 mg cortrosyn injection. Draw 30 and 60 min after injection. Order Cortisol if more or different draw times are requested, noting collection time in tracking. This test does NOT include ACTH levels. An ACTH may be ordered along with an ACTH stimulation Test. Check ACTH specimen requirements if ACTH test is ordered.

Processing: Cent. refrigerate.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL Serum Alternative Collection: RED SST Volume: 1.0 mL Serum

TAT: Report available: 3-5 days Test Schedule: Set up: 3 days a week, p.m Method: Immunofluorescence Assay Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130 Coxiella Burnetii Phase 1 and 2, IgG, IgM Clinical Data: Caused by infection with rickettsiae agent, Coxiella burnetti, Q Fever is characterized by fever with interstitial pneumonitis. Sixty percent of infected individuals are asymptomatic while other infected individuals may die from complications

Specimen Stability: Room temperature: 4 days Refrigerated: 7 days Frozen: 30 days Test Code: SJC TC 4085N

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day

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Test Schedule: Monday-Friday Evening Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: C-Peptide is useful in the evaluation of pancreatic beta cell function and for determining the source of insulin in patients with hyperinsulinemic hypoglycemia.

Patient Preparation: Fasting specimen is preferred C-Peptide Specimen Stability: Room temperature: 24 hours Refrigerated: 7 days Frozen -20° C: 28 days Frozen -70° C: 12 months Test Code: CHANTILLY TC 372

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Freeze Transport: Transport frozen on ice

Preferred Collection/Volume: RED SST Volume: 0.5mL s Alternative Collection: RED 10 Volume: 0.2mL s

TAT: 24 Hours C-Reactive Protein Test Schedule: Daily Method: Turbidometry Test Facility: AWL

Processing: Cent. Refrigerate if held.

Preferred Collection/Volume: RD10/1.0 mL s Alternative Collection: SSRED5, GNS5 (plasma) /o.5 mL serum

TAT: 7 Days Test Schedule: Wednesday C-Reactive Protein High Method: Chemiluminescent Immunoassay Sensitivity Test Facility: AWL

ADD ON STABILITY: 3 Days at 2-8¦C.

Processing: Centrifuge within 1 hour of collection, aliquot, refrigerate if held. Freeze after 72 hours.

Preferred Collection/Volume: DO NOT USE PRESERVATIVES. Keep refrigerated during collection. 24 Hour Urine Container Volume: 3.0 mL 24-Hour Urine

TAT: Report available: Next Day Creatine 24 Hour Urine Test Schedule: Monday-Friday, Afternoon Method: Colormeteric, Enzymatic Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Creatine, produced by the liver, is essential for metabolism of the brain and muscles. Creatine is converted to creatinine and excreted.

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Specimen Stability: Room Temperature:4 hours Refrigerated: 1 days Frozen: 3 Months Test Code: CHANTILLY TC 0966

Processing: Mix specimen well, aliquot and freeze immediately. Record total volume on aliquot. Transport: Transport frozen on ice

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p Alternative Collection: RED SST or serum acceptable Volume: 0.2mL s

TAT: Stat: 1 Hour Routine: 24 hours Test Schedule: Daily Creatine Kinase Method: Photometric, Creatine Phosphate + ADP Test Facility: SMC, INT, AWL

CKMB will be done if CK is greater than 99 U/L. Add-on testing stability: 48 hours TESTED AT: SMC, INT, (AWL CK ONLY)

Processing: Centrifuge. Transport: to testing location as noted on the specimen label.

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5 mL p Alternative Collection: RED SST or serum acceptable Volume: 0.2 mL s

TAT: Stat: 1 Hour Routine: 24 hours Test Schedule: Daily Creatinine Method: Enzymatic Colorimetric Test Facility: All: SMC, AWL, INT, BVT, CAS, LVK, SKY, NLR, RKW, SAL, VAN, DIV, SST, TWA, ORC

iSTAT Testing Performed by LAB Staff: Whole blood from unspun GNS5 acceptable for testing.

Processing: Centrifuge. Refrigerate if held.

Preferred Collection/Volume: 24 Hr urine (No preservative)/ 3.0 mL u Alternative Collection: NO Random Collections/ 2.0 mL u

TAT: 24 hours Creatinine 24 Hour Test Schedule: Daily Urine Method: Enzymatic Colorimetric Test Facility: AWL

Processing: Mix sample well and aliquot. Record total volume on aliquot.

Preferred Collection/Volume: LIGREEN4/ 4.0mL bf Alternative Collection: RD10/ 1.0mL bf

TAT: Stat: 1 Routine: 24 hours Test Schedule: Daily

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Method: Enzymatic Colorimetric Test Facility: SMC, AWL

Creatinine Body Fluid Use separate LIS requests if both blood and body fluids are ordered.

Processing: Refrigerate if held.

Preferred Collection/Volume: 24 hr Urine (no preservative) AND GNS5/ 4.0U/0.5P Alternative Collection: 12x75 aliq. for Urine/ 1.0U/0.2P

TAT: 25 Hours Test Schedule: Daily Method: Enzymatic Colorimetric Creatinine Clearance Test Facility: AWL CC combines with CC1. CC is the 24 hour urine creatinine and CC1 is the serum creatinine. Collect serum/plasma within 48 hrs of urine collection.

Processing: Record collection interval hrs. Measure and record total vol. Aliquot to 12x75 tube. Refrigerate.

Preferred Collection/Volume: AP99 with 12x75 aliq./ 5.0 mL u Alternative Collection: / 1.0 mL u

TAT: Stat: 1 Hours Routine: 24 Hours Test Schedule: Daily Creatinine Urine Method: Enzymatic Colorimetric Test Facility: State Only: SMC, BVT, INT AWL

Processing: Aliqout to 12x75 tube. Refrigerate.

Preferred Collection/Volume: SSRED5 for Cortisol and LV5 4 for ACTH of each/ 1.5 mL Alternative Collection: RD10 for cortisol/ 1.0 mL

TAT: 7 Days Test Schedule: ACTH - On Wed Only Daily Method: Chemiluminescent immunoassay f Test Facility: AWL CRH Stimulation Test Corticotropin Releasing Hormone (CRH) Stimulation test comprises measurement of ACTH and cortisol levels at baseline,15,30, and 60 minutes after injection of the CRH. The cortisol level is collected in a SSRED5, the ACTH level is collected in a LV5.

Processing: Cortisol: Centrifuge SSRED5, Aliquot if RD10. Refrigerate. ACTH;: Centrifuge LV5 Aliquot plasma into ACTH tube. Freeze and transport on ice. Do not allow to thaw.

Preferred Collection/Volume: 1 RD10 and 1 LV5/ 5.0S/2.0P Alternative Collection: / 4.0S/0.5P

TAT: 24 hours Test Schedule: Daily Method: Cold Precipitation Test Facility: AWL

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This test includes Qual Cryoglobulin and a Qual Cryofibrinogen. Do not order Quant Cryoglob(RCL) directly. Draw the RD10 into a warmed tube (37-42 C). Allow the RD10 to clot at 37-42 C. Cryoglobulin Processing: Keep both specimens at 37-42C until they are ready to centrifuge. Cent and pour serum and plasma into the appropriately labeled aliquot tubes. Transport at room temp. AWL: Place all tubes in 37C incubator in SAM/TOX upon arrival.

Preferred Collection/Volume: Draw specimens in warm tubes (37-42° C). RED 10 Volume: 3 mL Serum

TAT: 4 Days Test Schedule: Monday-Sunday Evening Method: Cold Percipitation Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: The Cryocrit is primarily intended for following a patient with previously defined and quantitated cryoglobulins. The cryocrit may consist of cryoglobulins, fibrins or mixtures of cryoglobulins and fibrin. If not previously characterized, consider ordering test code 37358X, Cryoglobulins Quant Cryoglobulin Screen with Reflex to Cryoglobulin Profile, Serum.

Patient Preparation: A fasting sample is required

Specimen Stability: Room temperature: 72 hours Refrigerated: Unacceptable Frozen: Unacceptable Test Code: Quest TC 4003

Processing: Allow blood to clot at 37-42° C for 1 hour. Keep specimens at 37-42° C until they have been centrifuged and the serum has been separated from the cells. Transfer serum to clean, Referred Tests aliquot tube. DO NOT refrigerate. Transport: Send Room Temperature

Preferred Collection/Volume: CSF submitted in sterile container Volume: 0.5mL CSF

TAT: 24 hours Test Schedule: Daily Test Method: Lateral Flow Cryptococcal Antigen Testing Location: Airport Way Regional Laboratory CSF Positive Cryptococcal Antigen results will reflex semi-quantitative testing (titer),

Processing: Transportation: Transport on ice to AWL in Red Tote

Preferred Collection/Volume: RED10 Volume: 1.0mL s Minimum Volume: .5mL s Cryptococcal Antigen Serum is the only acceptable sample for this test Serum TAT: 24 hours Test Schedule: Daily Test Method: Lateral Flow Testing Location: Airport Way Regional Laboratory

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Processing : Centrifuge and aliquot Transportation: To AWL in RED tote

Non Orderable test. Positive Cryptococcal Antigen results wil reflex semi- Cryptococcal Antigen quantitative testing (titer). Titer CSF

Non Orderable test. Positive Cryptococcal Antigen results will reflex semi- Cryptococcal Antigen quantitative testing (tite). Titer Serum

Preferred Collection/Volume: Stool collected and placed in Parasite Collection Kit/ to fill line Alternative Collection: n/a

TAT: 1 - 2 Days Test Schedule: Mon - Sat Method: DFA Test Facility: AWL

Clinical Data: n/a Patient History: n/a Cryptosporidium Exam, Stool Patient Preparation: Stool collected and placed in Parasite collection kit filled to line. Follow directions provided by the laboratory. Labeling: Date and time of collection

Rejection Criteria: Expired transport media. Specimen submitted without formalin vial. Overfilled collection vial. No observable specimen in collection vial.

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: 2 mL body fluid, minimum 0.5 mL Sodium heparin Alternative Collection: Lithium heparin or EDTA accepted but may interfere with test.

TAT: 1 day Test Schedule: Daily Method: Microscopy Crystal Exam Body Test Facility: INT, AWL Fluid Preparation: Clinician collected. Labeling: If shared with cell count, please label with shared specimen sticker.

Processing: Refrigerate and transport to testing laboratory. Stable 12 hours. Transport: Refrigerate until exam.

Preferred Collection/Volume: LAV EDTA Volume: 4.0 mL Whole Blood

TAT: Reported 24 hours Test Schedule: Performed 7 days/week Method: Colorimetry Test Facility: Legacy Central Lab 1225 NE Second Ave Portland, OR 97232.

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Rejection Criteria If minimum volume is not met, call the laboratory to find out if the test can be performed. LEGACY CS: 503-413-1234. NO TEST CODE Cyanide Level Processing: PROCESS STAT. Do not centrifuge. Cab directly to Legacy Central Lab with completed Legacy Requisition and Specimen Transport Form 23-09-204. Dispatch Specimen Transport Form to AWL. Transport: Transport on ice.

Preferred Collection/Volume: SSRED5/ 1.0 mL s Alternative Collection: RD10/ 1.0 mL s

TAT: 24 Hours Cyclic Citrulline Test Schedule: Mon - Fri Peptide Method: Multiplex flow Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot if RD10. Refrigerate if held.

Preferred Collection: Stool Volume: Collected and placed in Parasite Collection Kit to fill line

TAT: 1 - 2 Days Test Schedule: Mon - Sat Method: Microscopy Cyclospora Identification Exam Testing Location : AWL Rejection Criteria:

{ Expired transport media

{ Overfilled collection vial

{ No observable specimen in collection vial

Processing: Hold and transport at room temperature

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL Absolute Minimum Volume: 1.0 mL

TAT: 24 Hours Test Schedule: Daily Method: Immunoassay Cyclosporine Level Test Facility: AWL

Draw 12 hrs. after last dose. May be collected at other times if directed by pharmacist or clinician.

Processing: Do not cent. LAB CENTRAL: Refrigerate.

Preferred Collection: Whole blood LAV EDTA

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Volume: 4.0 mL Minimum Volume: 1.0 mL

TAT: 2-6 Days Testing Schedule: Wednesdays and Fridays Testing Method: Invader Assay Testing Location: Airportway Laboratories Molecular Diagnostics Department Cystic Fibrosis (CF) Rejection Criteria: Heparinized or Frozen specimens Molecular Patient History: Required history: Ethnicity, Pregnancy status and gestational age of fetus, family history of CF, has patient been previously tested for CF.

Processing: Do not Centrifuge. Transport: Transport refrigerated. Do not freeze.

Preferred Collection/Volume: Sputum, induced sputum, tracheal aspirates, tracheostomy specimens, bronchial washings, bronchial alveolar lavages submitted in sterile leakproof container/ >1mL Alternative Collection: Deep Throat collection submitted using a Liquid Amies Transport swab (E-Swab)

TAT: 3 - 14 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: This culture is for cystic fibrosis patients only. Cystic Fibrosis Culture No Gram Stain is performed with this test. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired collection/transport device Greater than 24 hours post collection

Processing: Refrigerate Transport: Send on Ice

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: 3 Days Test Schedule: Tuesday, Friday Morning Method: Enzyme-Linked Immunosorbent Assay Cysticercosis Antibody Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave, Suite 200 Cypress, CA 90630

Clinical Data: Cysticercosis is caused by infection with the larval form (cysticercus) of the pork tapeworm Taenia solium. Clinical manifestations of cysticercosis most commonly result from the lodging of cysticerci in brain and neural tissue. Common symptoms of neurocysticercosis include seizures and convulsions. Antibodies from other parasitic infections, especially echinococcosis,

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may cross-react in the Cysticercus IgG ELISA.

Specimen Stability: Room Temperature: 5 days Refrigerated: 2 weeks Frozen: 1 month Test Code: CHANTILLY TC 23322 TO FOUCS TC 40350

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: DO NOT USE PRESERVATIVES. Keep refrigerated during collection. 24 Hour Urine Container Volume: 2 mL 24-Hour Urine

TAT: Report available: 5 Days Test Schedule: Tuesday, Thursday Morning Method: Liquid Chromatography/Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Cystinuria is an autosomal recessive disease in which dibasic amino acids, Cystine 24 Hour Urine including cystine, are excreted in excess. Cystine kidney stones account for approximately 1-3% of all kidney stones. Age of onset is often younger than with other types of kidney stones and the recurrence is approximately one-third.

Patient's age is required for correct reference range. Specimen Stability: Room Temperature: unacceptable Refrigerated: unacceptable Frozen: 2 months Test Code: CHANTILLY TC 6016 TO SJC TC 10947X

Processing: Mix specimen well, aliquot and freeze immediately. Record total volume on aliquot. Transport: Transport frozen on ice

Specimen Preparation: Throat swab or tissue placed in a Viral Transport Media Whole Blood: Lavender (EDTA) or Pink (K 2 EDTA). Volume: 5 mL Minimum Volume: 1 mL

Fluid specimen : Bronchoalveolar Lavage (BAL), or urine. Transfer specimen to a Cytomegalovirus sterile container. Culture Volume: 2 mL Minimum Volume: 0.5 mL

TAT: Report available in 1-5 days Testing Location: ARUP Laboratories 500 Chipeta Way Salt Lake City, UT 84108-1221 Test Code: 0065004

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Specimen Stability: Room Temperature: 2 hours Refrigerated: 72 hours Frozen: Unacceptable Rejection Criteria: CSF, rectal swab, or stool. Whole blood in viral transport media. Calcium alginate, eSwab, dry, or wood swabs. http://www.aruplab.com/testing

Note: CSF specimens are not acceptable for Cytomegalovirus Culture, PCR is recommended.

Processing: Refrigerate specimen Transport: Send speicmen on Ice to Airport Way Lab Referred Test Department

Cytomegalovirus Culture ( C CMV, Cytomegalovirus Culture)

Preferred Collection: BLUE CIT Tube must be filled to minimum fill indicator Volume: Full tube (for testing on site) 0.5 mL plasma minimum for testing off site.

TAT: 2 hours Test Schedule: Daily Test Method: Immunotubidometric Testing Location: AWL, INT, LVK, NLR, SMC, WMC Specimen Stability: Room Temperature: Plasma 8 hours Frozen: 1 month

D-Dimer Preparation: If using a butterfly, a citrate discard tube must be used first, when citrate is the first tube collected.

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Process all D-dimer specimens as STAT. Centrifuge per coag specimen protocol. Aliquot plasma if tested off site. Note: Specimen must be centrifuged and processed as a STAT specimen. Recollection is recommended if the specimen was not spun within 4 hours of collection. Transport: Send STAT to testing site.

Preferred Collection/Volume: DO NOT USE PRESERVATIVES. Keep refrigerated during collection. 24 Hour Urine Container Volume: 2.0 mL 24-Hour Urine

TAT: Report available: 2 Days Delta Aminolevulinic Test Schedule: Monday, Wednesday, Friday Afternoon Acid 24 Hr Urine Method: Colorimetric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Specimen Stability: Room Temperature: Unacceptable

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Refrigerated: 14 days Frozen: 1 month Test Code: CHANTILLY TC 219

Processing: Mix specimen well, aliquot and wrap in foil to protect from light. Record total volume on aliquot. Transport: Transport on ice.

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL Whole blood

Test Facility: Kaiser Regional Molecular Genetics Lab 5755 Cottle Rd. Bldg #6 Dentatorubral San Jose, CA 95123 (408)972-3308. Pallidoluysian Atrophy (DRPLA) Gene Analysis Refrigerate if held overnight. Ship Room Temperature

Processing: Do not Centrifuge. Transport: Send Room Temperature

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 3.0 mL Serum

TAT: Reports in 2 days. Test Schedule: 1 day a week Method: Extraction, Chromatography, Radioimmunoassay Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: DOC is a precursor of corticosterone and aldosterone. The elevations of DOC Deoxycorticosterone observed in patients with 11-hydroxylase deficiency (the second leading cause of congenital adrenal hyperplasia) may reflect the hypertension observed. Patients with DOC-secreting tumors also exhibit hypertension.

Specimen Stability: Room temperature: 48 Hours Refrigerated: 7 Days Frozen: 2 Years Test Code: SJC TC 6559X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 3.0 mL Serum

TAT: Report available: 2 Days Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Level Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Serum level of Desipramine is used to assess patient compliance and to make dosage adjustments to achieve therapeutic levels, while avoiding toxicity. Desipramine is a tricyclic antidepressant most frequently used in the treatment of depression.

Patient Preparation: Patient preparation: Collect as a trough or at least 12 hours after last dose.

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Specimen Stability: Room Temperature: 5 days Refrigerated: 7 days Frozen: 1month (-20c) Test Code: CHANTILLY TC 893

Processing: Separate from cells as soon as possible after clotting. Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: SSRED5/ 1.0 mL s Alternative Collection: RD10 (aliquot)/ 0.3 mL s

TAT: 24 Hours Test Schedule: Daily Dexamethasone Method: Chemiluminescent Immunoassay Suppression Test Facility: AWL

Patient is instructed by clinician to take Dexamethasone orally at 11pm to midnight and report to lab in the AM.

Processing: Cent. and refrigerate

Preferred Collection/Volume: RED 10 Volume: 1.0mL s Alternative Collection: RED SST Volume: 0.5mL s

TAT: 7 Days DHEAS Test Schedule: Friday Method: Chemiluminescent Immunoassay Test Facility: AWL

Processing: Cent. Aliquot. Refrigerate up to 2 days. Freeze for longer periods of storage

Preferred Collection/Volume: Peritoneal dialysate inoculated into Blood Culture bottles: One aerobic and one anaerobic bottle / 8 to 10 mLs each bottle. An additional specimens for Gram Stain must also be submitted, place >0.5mL in a sterile leakproof container and submit with bottle. Alternative Collection: Sterile leakproof container/ > 1 mL; SPS tube completely filled

TAT: 5 - 10 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Dialysate Fluid Culture Clinical Data: n/a Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Greater than 24 hours post collection expired collection/transport containers SPS tube less than maximum fill Any additive tube other than SPS

Processing: Hold at room temperature

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Transport: Room temperature

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Report available: 3 Days Test Schedule: Friday Morning Method: Liquid Chromatography/Tandem Mass Spectrometry Calculation Test Facility: Quest Diagnostics Nichols Institute Valencia 27027 Tourney Road Valencia, CA 91355-5386

Clinical Data: Therapeutic drug monitoring of anxiolytic drug. Diazepam Level Nordiazepam is the active metabolite of the parent drug. Elimination half-life is 20-90 hours. Patient Preparation: Collect at trough concentration, i.e., immediately before the administration of the next dose.

Specimen Stability: Room Temperature: unacceptable Refrigerated: 5 days Frozen: 14 days Test Code: CHANTILLY TC 90853 TO VALENICA TC 90853

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Freeze. Transport: Transport frozen on ice

Preferred Collection/Volume: Green 3 Sodium Heparin/ 3cc MINIMUM VOLUME 1CC Alternative Collection: n/a

TAT: Routine: 14 - 21 days Test Schedule: Mon-Fri Method: In situ hybridization on metaphase and interphase cells Test Facility: AWL

Digeorge VCF Mutation Clinical Data: If ordered in combination with a chromosome study, only 1 specimen is needed. (22q11) Analysis, Fish Include clinical indication. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: ** No Gel barrier tubes ** Do not Centrifuge. Hold at Room temperature. Do NOT reject centrifuged specimen; contact department at 503-258-6723 (Tie Line: 35-6723) Transport: Room Temperature.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: 4 Days Digitoxin Level Test Schedule: Tuesday, Thursday Afternoon Method: Immunoassay Test Facility: NMS LABS 3701 Welsh Road Willow Grove, PA 19090

Clinical Data: Digitoxin is a cardiovascular drug; it is used for therapeutic monitoring.

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Specimen Stability: Room Temperature: 5 days Refrigerated: 7 days Frozen: 2 months (-20c) Test Code: CHANTILLY TC 900821 TO NMS TC 1613SP

Processing: Centrifuge ASAP and aliquot into referred tests aliquot tube. Transport: Refrigerate if held

Preferred Collection: RED 10 Alternate Collection: LAV EDTA is acceptable if tested at AWL Volume: 0.5 mL Minimum Volume: 0.2 mL Digoxin Level Note: Gel barrier tubes are not acceptable.

TAT: 24 hours Test Schedule: Daily

Note: Preferred draw time is trough - immediately before next dose or 6 hrs post dose.

Processing: Centrifuge and aliquot. Refrigerate if held .

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.5 mL Serum

TAT: Report available: 4 Days Test Schedule: Monday-Friday Evening Method: Extraction • Radioimmunoassay • Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Dihydrotestosterone Clinical Data: DHT is a potent androgen derived from via 5-alpha-reductase (DHT) activity. 5-alpha-reductase deficiency results in incompletely virilized males (phenotypic females). This diagnosis is supported by an elevated ratio of testosterone to DHT.

Specimen Stability: Room Temperature: 48 hours Refrigerated: 1 week Frozen: 2 years Test Code: CHANTILLY TC 69962

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerated

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

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TAT: Report available: 4 days Test Schedule: Mon-Sat evening Method: Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: This test is used to measure the bio-active form of Vitamin D. This test is also used in the differential diagnosis of hypocalcemia and to monitor patients with renal osteodystrophy or chronic renal failure.

Dihydroxyvitamin D Specimen Stability: 1,25 Level Room temperature: 28 days Refrigerated: 28 days Frozen: 28 days Rejection Criteria: Grossly hemolyzed, lipemic, or icteric specimens, or plasma samples are unacceptable for this test If sample is submitted with less than 1.1 mL and needs to be repeated the sample will be canceled with the comment "TNP-Initial testing necessitated a repeat, but there was insufficient sample to perform repeat". Test Code: CHANTILLY TC 16558

Processing: Centrifuge and aliquot into referred tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday, Wednesday, Friday Morning Method: Enzyme Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Diphtheria Antibody Chantilly, VA 20153 Specimen Stability: Room Temperature: 4 days Refrigerated: 7 days Frozen: 30 days Test Code: CHANTILLY TC 4865

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: Pink EDTA Volume: 5.0 mL Alternative Collection: LAV EDTA RED 10 (do not centrifuge) Direct Absolute Minimum Volume: 1.0 mL

For Direct Antihuman Globulin Test (DAT). Sometimes referred to as "Coomb. DO NOT USE FOR CORD BLOOD.

Preferred Collection/Volume: Tissue placed into Michel's Fixative by provider at collection

TAT: 2 days Test Schedule: Monday-Friday Test Method: Direct Tissue Immunofluorescence

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Testing Location: UC DAVIS DERMATOPATHOLOGY SERVICE Department of Dermatology 3301 CStreet, Suite 1450 Sacramento, California 95816 Telephone (916) 734-6373 Direct Tissue Toll Free (866) 323-9061 Immunofluorescence Fax (916) 703-5159

Cross reference Co-Path Accession number into NW PathNet Order Notes. Transportation: Room Temperature

Preferred Collection/Volume: Random Urine in AP99 Volume: 1.0 mL

TAT: Report available: 4 Days Test Schedule: Tuesday, Thursday Morning Method: High Performance Liquid Chromatography Test Facility: NMS LABS 3701 Welsh Road Diuretic Profile Willow Grove, PA 19090

Clinical Data: Includes: Chlorothiazide, Hydrochlorothiazide, Hydroflumethiazide, Methyclothiazide, Trichlormethiazide, Benzthiazide, Chlorthalidone, Metolazone, Furosemide

TC# 44327Quest to NMS TC# 9318U

Transport: Refrigerate if held.

Preferred Collection/Volume: Grey Na Fluor Volume: 1.0 mL p

TAT: 4 Days Test Schedule: Monday, Thursday Method: Enzymatic Test Facility: Mayo Medical Laboratories via Quest Chantilly 3050 Supewrior Drive NW Rochester, MN 559065

Clinical Data: Useful For Suggests clinical disorders or settings where the test may be helpful An adjunct to urine D-lactate (preferred), in the diagnosis of D-lactate acidosis D-lactate is produced from carbohydrates that were not absorbed in the small intestine by bacteria residing in the colon. When large amounts are absorbed it can cause metabolic acidosis, altered D-lactate mental status (from drowsiness to coma), and a variety of other neurologic symptoms, in particular dysarthria and ataxia. Although a temporal relationship has been described between elevations of plasma and urine D-lactate and the accompanying encephalopathy, the mechanism of neurologic manifestations has not been elucidated. D-lactic acidosis is typically observed in patients with short-bowel syndrome and following jejunoileal bypass resulting in carbohydrate malabsorption. In addition, healthy children presenting with gastroenteritis may also develop the critical presentation of D-lactic acidosis. D-lactate is readily excreted in urine, which is the preferred specimen for D-lactate determinations. Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.0.0-0.25 mmol/L Interpretation Provides information to assist in interpretation of the test resultsIncreased levels are consistent with D-lactic acidosis. However, because D-lactate is readily excreted, urine determinations are preferred. Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substancesUrine is the

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preferred specimen to determine D-lactate. The test performed was D-Lactate. This is a product of bacterial overgrowth in the gastrointestinal tract. It should not be confused with L-lactate which accumulates in some metabolic acidosis.

Specimen Stability: Frozen: 365 days Ambient: 7 days Refrigerated: 7 days TC# MAYO TC DLAC Transport frozen to Quest

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: RD10/ 2.0 mL S

TAT: 24 hours Test Schedule: Mon - Fri DNA Antibody (Double- Method: Immunofluorescence stranded) Test Facility: AWL

Processing: Cent, Aliquot, Refrigerate

Preferred Collection/Volume: LAV EDTA Volume: 6.0 mL whole blood Other tissues may be acceptable for DNA storage. Please call the lab at 503-494-5400 before sending specimens other than blood

TAT: Report Available: 2 weeks Test Schedule: Daily, Monday - Friday Test Facility: OHSU Molecular Diagnostic Center Marquam Plaza 2525 SW 3rd Avenue, Suite 350 Portland, OR 97201

DNA Banking, Clinical Data: Interpretation: Molecular Isolation or DNA yield is quantitated and purity is estimated by spectrophotometric evaluation of 260 nm/280 nm ratio. Extraction Comments: Purifed high molecular weight DNA for storage. DNA storage is applicable in situations where a confirmed or suspected genetic condition is evident and the possibility of future testing will be critically dependent on the analysis of specific samples. This procedure is particularly important in cases where an affected individual may be medically unstable. Please call the Lab for further information at 503-494-5400 before submitting the specimen. CONSENT FORM REQUIRED. Patient History: CONSENT FORM REQUIRED.

Test Code: OHSU TC 102-7001

Processing: Do not Centrifuge. Send samples to arrive Monday through Friday ONLY

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: 3 days Test Schedule: Set up: Monday-Friday Morning Method: Test Facility: Quest Diagnostics Nichols Institute Valencia 27027 Tourney Road Valencia, CA 91355-5386

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Clinical Data: Documents Streptococcal Infection

Specimen Stability: Room temperature: 48 hours Refrigerated: 8 days Frozen: 90 days Reject Criteria: Dnase B Antibody Received room temperature Reject Thaw/Other Test Code: CHANTILLY TC 2952 TO VALENCIA TC 2351

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: Two 5.0 mL red top tubes Do not use SST or Corvac tubes. See WIs 3.1-15-311 for detailed instructions. Alternative Collection: n/a

TAT: Same day Test Schedule: Monday – Friday, during laboratory operating hours Test Method: PCH /warm and PCH/cold Test Facility: Must be performed at a testing location with immediate access to an incubator and refrigerator All collection, processing and test interpretation must be done on site. Donath-Landsteiner, Incubated, Warm and Clinical Data: n/a Cold Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Blood drawn in SST or Corvac tubes are rejected

Processing: See WIs 3.1-15-311 Transport: Test performed on site

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 3.0 mL Serum

TAT: Report available: 2 Days Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Doxepin Level Clinical Data: Doxepin in a tricyclic Antidepressant. Its metabolite, Nordoxepin (Desmethyl Doxepin) is also pharmacologically active.

Specimen Stability: Room Temperature: 5 days Refrigerated: 7 days Frozen: 1 month Test Code: CHANTILLY TC 894

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held

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Preferred Collection/Volume: Meconium (First Newborn Stool Specimen) Volume: 21.0 grams

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Drug of Abuse Screen, Meconium Clinical Data: Includes: , Cocaine, and Opiates (Codeine and Morphine).

Specimen Stability: Room Temperature: 48 hours Refrigerated: 7 days Frozen: 30 days (-20c) Test Code: CHANTILLY TC 267164

Transport: Refrigerate if held.

Preferred Collection/Volume: AP99 Random urine/ 50 mL u Alternative Collection: / 20 mL u

TAT: 24 hours Test Schedule: Daily Method: Immunoassay Drug Screen Test Facility: AWL Abbreviated, Urine (Amp,Thc,Coc,Opi,Oxy) Clinical Data: Positive cannabinoid screen will reflex THC quantitative confirmation

This panel includes Amphetamines, Cocaine metabolite, Opiates, Cannabinoids, Oxycodone and Creatinine

Processing: Refrigerate if held overnight

Preferred Collection/Volume: AP99 Random urine/ 50 mL u Alternative Collection: / 20 mL u

TAT: 24 Hours Test Schedule: Daily Method: Immunoassay Drug Screen Basic, Test Facility: AWL Urine Test includes screens for Amphetamines, Barbiturates, Benzodiazepine, Cannabinoid, Cocaine metabolite, Ethanol, Methadone, Opiate, Oxycodone and Creatinine. Positive cannabinoid screen will reflex THC quantitative confirmation

Processing: Refrigerate if held overnight

Preferred Collection/Volume: AP99 Random urine/ 50 mL u Alternative Collection: / 20 mL u

TAT: 24 Hours Drug Screen Test Schedule: Daily Comprehensive, Urine Method: Immunoassay Test Facility: AWL

Test includes: Amphetamines, Barbiturates, Benzodiapines, Cannabinoids, Cocaine metabolite, Opiates, Urine organic base screen, Methadone, Oxycodone, OTC medications, Prescription drugs, and Creatinine

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Processing: Refrigerate if held overnight

Preferred Collection: AP99 Volume: 50 mL urine (Absolute Minimum Volume: 10 mL urine)

TAT: 1 Hour STAT Test Schedule: Daily Method: Immunoassay Test Facility: Sunnyside Medical Center and Westside Medical Center Add on Stability: 2 days if refrigerated Drug Screen, Urine (Amp, Meth, Bar, Bzd, Note: FOR EMERGENCY DEPARTMENT AND URGENT INPATIENT TESTING. Coc Met, Mtd, Opi, Point of care drug screen test that screens for amphetamine, , Oxy, Pcp, Propox, Thc, opiates, barbiturates, benzodiazepines, cocaine metabolite, cannabinoids Tca) (THC),oxycodone, methadone, (PCP), propoxyphene and tricyclic anti- depressants(TCA). Presumptive positives are not automatically confirmed. Drug classes reported as "Further Testing Required" will reflex confirmation testing. FOR LABOR AND DELIVERY AND NEONATAL TESTING-please see Drug Screen,Abbreviated

Processing: Refrigerate

Preferred Collection/Volume: REDSST Volume: 1.0mL s Alternative Collection: RED10

TAT: 1-3 days Test Schedule: Monday-Friday dsDNA Antibody Test Method: Multiplex Flow Immunoassay Testing Location: Airport Way Regional Laboratory

Processing: Centrifuge. Aliquot if collected in RED10 Transport: To AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: Yellow ACD Solution A or B Volume: 6.0 mL wb Absolute Minimum: 2-3mL

TAT: 2 weeks Test Schedule: Weekly or on demand; STAT testing available Test Facility: Molecular Diagnostic Center, OHSU 2525 S.W. 3rd Avenue, Suite 350 Portland, OR 97201 Duchenne, Becker Muscular Dystrophy Clinical Data: DMD/BMD constitute the most common forms of childhood muscular dystrophy. Dystrophin Mutation The presence of a deletion or a duplication is diagnostic and allows carrier testing for at-risk female relatives. Genetic counseling is indicated for affected individuals and their families. Please contact Analysis the laboratory at 503-494-5400 for more information or to request requisition forms. Reference Range: A dystrophin gene deletion or duplication is diagnostic of DMD or BMD. Absence of a deletion or a duplication does not confirm or rule out a diagnosis of DMD or BMD. Interpretation: Professional interpretation required. 65% of the DMD/BMD cases are due to a detectable deletion or a duplication of the dystrophin gene at Xp21. Molecular characterization of a deletion or a duplication allows a differential diagnosis of a DMD vs. BMD phenotype.

OHSU GENETIC FORMS CAN BE FOUND AT THE FOLLOWING LINK: http://www.ohsu.edu/pathology/wardman/forms/mdcreqslip.pdf

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Test Code: OHSU TC 102-7015

Processing: Do not centrifuge.

Preferred Collection: AP99 Random urine or UVAC Volume: 10 mLs

TAT: 1-4 hours Test Schedule: Daily Dysmorphic RBC, Method: Microscopy Urine Test Facility: Medical Office labs, SMC, WMC

Processing: Testing should be performed within 1 hour. If not possible, immediately place specimen on ice or refrigerate. Test within 4 hours. Transport: Tested on site.

Preferred Collection/Volume: Drainage, ear, ear canal, lesion, middle ear fluid: submitted using a Liquid Amies Transport swab (E-Swab) Alternative Collection: Fluid in sterile leakproof container/ >0.5 mL

TAT: 2 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: n/a Patient History: n/a Ear Culture Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired collection/transport device Greater than 48 hours post collection Dry Swab

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: 5 Days Test Schedule: Tuesday, Friday Morning Method: Enzyme-Linked Immunosorbent Assay Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave, Suite 200 Echinococcus Antibody Cypress, CA 90630 Clinical Data: Clinical Significance Echinococcus granulosus IgG detection is an important tool for diagnosing hydatid disease, since infected individuals do not exhibit fecal shedding of E. granulosus eggs.

Specimen Stability: Room Temperature: 7 days Refrigerated: 14 days Frozen: 30 days Test Code: CHANTILLY TC 34169 TO FOCUS TC 40430

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Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5 p Alternative Collection: RED SST Volume: 0.5 s

TAT: Stat: 1 Hour on site or 3 hours if performed off site Routine: 24 hours Test Schedule: Daily Electrolyte Panel Method: See Individual Assays Test Facility: Airport Way Regional Laboratory, Interstate, Beaverton, Cascade, Longview Kelso Medical Offices Sunnyside Medical Center

Fill tube completely

Processing: Centrifuge, DO NOT REMOVE CAP. Refrigerate if held.

Preferred Collection/Volume: Freeze sample immediately after collection Sterile Container Volume: 10 mL random liquid stool Alternative Collection: 10 mL aliquot of 24-hour, 48-hour, or 72-hour liquid stool specimens

TAT: Next Day Test Schedule: Monday-Friday Evening Method: Flame Photometric Coulometric Titration Electrolytes Fecal Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Patient Preparation: Keep refrigerated during collection

Specimen Stability: Frozen Only Test Code: Quest TC 4945

Transport: Transport frozen on ice.

Preferred Colllection/Volume: SSRED Collection Container Must Be Submitted-Do Not Aliquot Volume: 2.0mL s Absolute Minimum Volume: 0.5mL s

TAT: 24 Hours Employee Health Screen Test Schedule: Monday through Friday 1 Method: Immunoassay Test Facility: Airport Way Regional Laboratory

HIV 1/2 Ab Confirmation reflex testing of all reactive results performed at Airport Way Regional Laboratory.

FOR EMPLOYEE HEALTH USE ONLY

Processing: Centrifuge. Do Not Aliquot

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Transportation: Transport to AWL in blue tote. Refrigerate if held.

Employee Health Screen 1 ( 87389A EAP, Employee Health Screen 1, EHS)

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next day Test Schedule: Monday-Friday Morning Method: Immunofluorescence Assay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Endomysial Antibody Clinical Data: Clinical Significance A positive IgA endomysial antibody result supports the IgA Screen diagnosis of celiac disease.

If Endomysial Antibody Screen IgA is abnormal, Endomysial Antibody Titer will be performed at an additional charge (CPT code(s): 86256). Specimen Stability: Room temperature: 4 days Refrigerated: 21 days Frozen: 21 days Test Code: CHANTILLLY TC 15064

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 1mL Serum if additional sample must be sent.

TAT: Quest reflex-Unorderable Test Schedule: Set up: Mon-Fri Morning; Report available: Next day Method: Immunofluorescence Assay (IFA) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Patient Preparation: Quest reflex-Unorderable by KP Staff<> Endomysial Antibody Screen (IgA) is abnormal, Endomysial Antibody Titer will be performed at an additional charge (CPT code(s): Endomysial Antibody 86256). IgA Titer Labeling: Do not order. Quest reflexes titer from postive screen.

Specimen Stability: Room temperature: 4 days Refrigerated: 21 days Frozen: 21 days Reject Criteria: Gross hemolysis • Gross lipemia Test Code: Quest TC 15064

Processing: Centrifuge and aliquot into a referred tests aliquot tube Transport: Refrigerate if held.

Preferred Collection/Volume: Random Stool in Plastic Sterile Leak Proof Container Volume: 1.0 gram

TAT: Report available: 3 Days Test Schedule: Monday, Wednesday Morning

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Method: Enzyme Immunoassay Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave, Suite 200 Cypress, CA 90630

Clinical Data: Clinical Significance The detection of E. histolytica antigen by immunoassay is a highly sensitive and specific test. It is useful in cases of amebiasis where ova and parasite examinations are inconclusive. This assay will detect only antigens of the pathogenic E. histolytica; it will not detect antigens of the nonpathogenic E. dispar. Entamoeba Histolytica Ag Specimen Stability: Room Temperature: 24 hours Refrigerated: 48 hours Frozen: 7 days (-20c) Test Code: CHANTILLY TC 11071 TO FOCUS TC 50105

Processing: Cary-Blair transport medium is not acceptable. Freeze and ship frozen Transport: Transport frozen on ice

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Reports in 1 day Test Schedule: Sets up 1 day a week. Method: Enzyme Linked Immunosorbent Immunoassay Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave. Cypress, CA 90630

Clinical Data: Clinical Significance Serologic studies may be helpful in the diagnosis of invasive Entamoeba Histolytica intestinal and extra-intestinal Ameviasis. Asymptomatic cyst passers usually have negative Antibody Assay serologic tests. Eighty-Five percent of patients with biopsy proven invasive intestinal Amebiasis are antibody positive whereas 95-100% of patients with extra intestinal Amebiasis are positive. Values may remain elevated 6 to 18 months following invasive disease.

Specimen Stability: Room temperature: 7 Days Refrigerated: 14 Days Frozen: 30 Days Test Code: SJC TC 34278N TO FOCUS TC 40105

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: WHITE PPT Volume 0.7 mL Plasma Alternative Collection: LAV EDTA Volume: 0.7 mL Plasma Lesion, rectal, and throat swabs also acceptable specimens. Volume: 0.5 mL

TAT: Reports in 1 day. Enterovirus by PCR Test Schedule: 7 days a week. Method: Real-Time PCR Reverse Transcriptase-Polymerase Chain Reaction Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave. Cypress, CA 90630

Clinical Data: Clinical Significance This assay is used to detect the presence of Enterovirus RNA (including enterovirus, echovirus, coxsackievirus, and poliovirus) in a patient's specimen. The

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use of PCR to assay for the presence of viral RNA in clinical specimens allows for rapid patient testing. With the inclusion of real time fluorescent detection techniques, an accurate and sensitive detection can be made.

Specimen Stability: Room temperature: 48 Hours Refrigerated: 7 Days Frozen: 30 Days Reject Thaw/Other Specimens containing heparin, Frozen glass containers Test Code: SJC TC 15082X TO FOCUS 47300

Processing: Centrifuge within 2 hours of collection. Transfer plasma to Referred Tests aliquot tube. Freeze. Rectal/throat swabs: Transport in viral transport media NOTE: Use EPC panel for CSF Transport: Refrigerate if held.

Preferred Collection/Volume: Collect 0.5 mL in a sterile CSF collection tube. 2nd or 3rd CSF collection preferred. Alternative Collection: Collect 0.3 mL CSF, 2nd or 3rd CSF collection preferred.

TAT: STAT: Reported within 3 hours of receipt Test Schedule: Sun - Sat Method: Real Time PCR Test Facility: Inpatient testing performed at NWMC Laboratories, all other testing performed at AWL: Microbiology

Clinical Data: N/A Enterovirus Detection Patient History: N/A CSF PCR Preparation: N/A Labeling: N/A

For specimens other than CSF (lesion, EDTA whole blood, rectal or throat swabs) see Enterovirus Culture or Enterovirus RNA by PCR.

Processing: Do not centrifuge. Transport: Keep specimens at 2 - 8 C until testing or freeze specimens if test will not be performed within 72 hours of collection. Do not freeze thaw the specimen more than two times. Inpatient testing performed at NWMC Laboratories; AWL for all other locations including OHSU.

Preferred Collection/Volume: Water Sample: 50mL in sterile leakproof container. Millipore paddle. Alternative Collection: n/a

TAT: 3 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Environmental Culture Clinical Data: This test is for surface and water testing Patient History: n/a

Patient Preparation: n/a Labeling: Label with sample identifier

Rejection Criteria: Greater than 24 hours post collection Specimen other than water or millipore paddle

Processing: Hold at room temperature

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Transport: Room temperature

Preferred Collection/Volume: LV5, LV3, or EDTA microtainer Alternative Collection : n/a

TAT: Stat 1 hr; routine 24hrs Test Schedule: Any day, during laboratory operating hours Test Method: 3-Part and 5-Part Diff Sysmex Cell Counters Test Facility: All KPNW labs performing hematology testing.

Eosinophil Count Clinical Data: n/a Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: Handle microtainer ASAP and test on-site when possible Transport: Transport at refrigerator temperature when sending to AWL.

Preferred Collection/Volume: Smear on glass slide. Alternative Collection: Swab or clean container with nasal discharge or sputum.

TAT: 24 hours Test Schedule: Daily Method: Microscopy Eosinophil Smear Nasal Test Facility: AWL

Processing: AWL: Smears must be prepared within 12 hours of collection. Refrigerate if delayed. Transport: Transport smears or specimens (keep refrigerated) to AWL.

Preferred Collection/Volume: 10 mL random urine in AP99 or UVAC

TAT: 24 hours Test Schedule: Daily Method: Microscopy Test Facility: AWL Eosinophil Urine Processing: Cytospin smears must be prepared within 2 hours of collection or 12 hours when refrigerated. Transport: Transport specimens collected between 8am Monday and 8pm Friday to AWL for smear preparation. If specimen will not reach AWL by 8pm Friday through 8 am Monday, prepare smears on site per 2.4-01-203.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 0.5 mL Serum

TAT: Report available: 3 Days Test Schedule: Tuesday-Friday Morning Method: Indirect Immunofluorescence Assay Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Clinical Significance Assay is for detection of antibodies to intercellular substance (ICS) in pemphigus, and to basement membrane (BM) in pemphigoid.

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Specimen Stability: Room Temperature: 4 days Refrigerated (cold packs): 7 days Frozen -20 degrees C: 30 days (glass tubes unacceptable) Frozen -70 degrees C: Indefinitely (glass tubes unacceptable) Test includes differentiation of antibodies to intercellular substance and basement membrane zone. Screens reflex to titers at an additional charge. The Intercellular Substance Antibody Screen may Epidermal Antibody reflex to the Intercellular Substance Antibody Titer (CPT: 86256). The Basement Membrane Zone Antibody Screen may reflex to the Basement Membrane Zone Antibody Titer (CPT: 86256). Test Code: CHANTILLY TC 37097 TO SJC TC 37097

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Rejection criteria: Gross hemolysis; gross lipemia, gross icterus. Transport: Refrigerate if held.

Preferred Collection/Volume: Formalin fixed paraffin embedded tissue block

TAT: Report available: 5 Days Test Schedule: Monday-Saturday Morning Method: Fluorescence In Situ Hybridization Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Epidermal Growth Chantilly, VA 20153 Factor Receptor, FISH Specimen Stability: Room Temperature: See instruction Refrigerated: See instruction Frozen: Unacceptable Test Code: CHANTILLY TC 19041X

Transport: Room temperature

Preferred Collection/Volume: RED SST Volume: 1.0 mL s

Panel includes: IgG and IgM to Viral Capsid Ag, IgG to Nuclear Antigens, IgG to Early Ag(d), and presence of Heterophil IgM

TAT: 24 hours Test Schedule: Mon - Fri Epstein Barr Ab Panel Method: Multiplex flow Immunoassay Test Facility: AWL

Panel includes: IgG and IgM to Viral Capsid Ag, IgG to Nuclear Antigens, IgG to Early Ag(d), and presence of Heterophil IgM

Processing: Centrifuge Tranpsortation : To AWL in blue tote

Preferred Collection/Volume: LAV EDTA Volume: 1 mL p

CSF, Eye fluid, Bronchoaveolar lavage, Bone marrow: Volume: 1.0 mL Blood collected in tubes with heparin anticoagulant is not acceptable for this test.

TAT: Next day

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Test Schedule: daily a.m. Test Method: Real-Time Polymerase Chain Reaction (RT-PCR) Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Significance Infection with EBV is common and is generally subclinical or presents as a self-limited illness. Reactivation of latent EBV in an immunocompormised person can lead to more serious results, including lymphoproliferative disorders or neurological disease. PCR methods may be useful in identifying EBV in a variety of clinical specimens.

Specimen Stability Bone Marrow Room temperature: 48 hours Refrigerated: 8 days Frozen: Unacceptable

Plasma, CSF, BAL, Eye fluids and Tissue Epstein Barr Virus, Room temperature: 48 hours DNA Refrigerated: 8 days Frozen: 30 days Reject Criteria • Frozen bone marrow • Sputum TC# 10186X

Processing: EDTA : Centrifuge within 2 hours of collection and aliquot into Referred Tests aliquot tube

CSF, Eye fluid, Bronchoaveolar lavage, Bone marrow: Collect in a sterile, plastic container Tissue: Collect in a sterile, plastic container CSF, Eye fluid, Bronchoaveolar lavage: Collect in a sterile, plastic container Transportation: Keep specimens cold and transport to AWL

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning Method: Immunoassay Erythropoietin Level Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Elevated levels of serum erythropoietin (EPO) occur in patients with anemias, due to increased red cell destruction in hemolytic anemia and also in secondary polycythemias associated with impaired oxygen delivery to the tissues, impaired pulmonary oxygen exchange, abnormal hemoglobins with increased oxygen affinity, constriction of the renal vasculature, and inappropriate EPO secretion caused by certain renal and extrarenal tumors. Normal or depressed

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levels may occur in anemias, due to increased oxygen delivery to tissues, in hypophosphatemia and in polycythemia vera.

Patient Preparation: Due to diurnal variation, it is recommended that specimens be collected between 0730 and noon.

Specimen Stability: Room Temperature: 10 days Refrigerated: 18 days Frozen: 28 days Test Code: CHANTILLY TC 427

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: RD10/ 1.5 mLs Alternative Collection: LIGREEN4/ 1.0 mLs

TAT: 24 hours Test Schedule: Mon - Sat Estradiol Level Method: Chemiluminscent Immunoassay Test Facility:

Processing: Centrifuge, Aliquot, Refrigerate. Freeze after 48 hours

Preferred Collection: RED 10 Alternate Collection: Serum Acceptable Volume: 3.0 mL Serum Pediatric Min Volume: 1.2 mL Serum

TAT: 4-7days Estradiol Ultra Testing Location: Sensitive, Peds Esoterix 4301 Lost Hills Road Calabasas Hills, CA 91301 TC# 500152

http://www.hemex.com/

Processing: Centrifuge specimen within 1 hour of specimen collection. Aliquot into a Referred Test aliquot container and freeze. Transportation: Freeze specimen and transport on ice.

Preferred Collection/Volume: RD10/ 2.0 mL s Alternative Collection: / 1.0 mL s

TAT: 2 Days Test Schedule: M, W, F

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Method: Immunoenzymatic Assay Test Facility: AWL Estriol Level Processing: Cent, aliquot and refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.5 mL Serum

TAT: Reports in 6 days Test Schedule: 6 days a week Method: Liquid Chromatograpy Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Test Components •Estradiol, Ultrasensitive, LC/MS/MS Estrogens Fract •Estriol, LC/MS/MS, Serum (Estrone, E2, E3) •Estrone, LC/MS/MS

Specimen Stability: Room temperature: 48 Hours Refrigerated: 7 Days Frozen: 2 Years -70 Degrees: 3 Years Reject Thaw/Other Thawed Serum, Received room temperature, Samples received in SST tubes Test Code: SJC TC 36742

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Freeze solid. Transport: Transport frozen on ice

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.5 mL Serum

TAT: Next day Test Schedule: Monday-Friday AM Method: Radioimmunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Estrogens Total Chantilly, VA 20153

Specimen Stability: Room Temperature: 24 hours Refrigerated: 7 Days Frozen: Not Established Test Code: CHANTILLY TC 439

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 0.5 mL Serum

TAT: Reports in 5 days Test Schedule: Sets up 6 days a week Method: Liquid Chromatography Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute

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33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Clinical Significance Estrone is primarily derived from metabolism of androstenedione in peripheral tissues, especially adipose tissues. Individuals with obesity have increased conversion of androstenedione to Estrone leading to higher concentrations. In addition, an increase in the ratio of Estrone to Estradiol may be useful in assessing menopause in women.

Specimen Stability: Estrone Level Room temperature: 48 Hours Refrigerated: 7 Days Frozen: 2 Years Reject Thaw/Other Received room temperature, SST Test Code: SJC TC 23244

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection: GRN Li Hep PST Alternative Collection: RED SS or Grey (Sodium Fouride) Volume: 0.5 mL plasma or serum ( Absolute Minimum Volume: 0.2 mL plasma or serum)

TAT: Stat: 1 Hour Ethanol Level Routine: 24 hours Testing Schedule: Daily Method: Colorimetric Testing Location: Sunnyside Medical Center and Westside Medical Center

Processing: Centrifuge specimen, do not uncap specimen. Refrigerate. Transportation: Send to appropriate Medical Center for testing. Rejection Criteria: Uncapped specimens

Preferred Collection: AP99 Random Urine Volume: 50 mL Minimum Volume: 5 mL

TAT: 24 hours Testing Location: Airportway Laboratory Ethanol Level Urine Note: Preferred test is Blood Alcohol performed at Sunnyside Medical Center and Westside Medical Center. Positive urine screens will automatically reflex the Ethanol Urine Confirmation test.

Processing: Refrigerate if held. Transportation: Send specimen refrigerated, in blue tote to Airport Way Lab for testing.

Preferred Collection/Volume: AP99 Random Urine/ 50 mL u Alternative Collection: / 10 mL u

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TAT: 24 Hours Test Schedule: Daily Method: GC-FID Test Facility: AWL Ethanol Urine Confirm To order a drug screen, see panels ALU or DSB

Processing: Refrigerate if held overnight

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: Next Day Test Schedule: Monday-Saturday Morning Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute Ethosuximide Level 14225 Newbrook Drive Chantilly, VA 20153

Specimen Stability: Room Temperature: 3 days Refrigerated: 7 days Frozen: 1 month Test Code: CHANTILLY TC 3852

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: Random urine collected in sterile urine cup Volume: 2 mL u

TAT: 2 days Test Schedule: Monday-Friday Test Method: Enzyme Immunoassay (EIA) Testing Location: NMS Labs

Purpose: Abuse Monitoring, Forensic Analysis, Compliance or Abuse Monitoring

Ethyl Glucuronide Stability Screen Room Temperature20 day(s) Refrigerated: 20 day(s) Frozen (-20 °C): 20 day(s) Rejection Criteria: None Transportation: Refrigerated TC# 9361U

Processing: Transportation: To AWL in blue tote

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Preferred Collection/Volume: LAV EDTA (Whole blood) Volume: 2.0 mL Whole Blood

TAT: 24 hours Test Schedule: 7 days/week Method: GC/FID Test Facility: Legacy Central Lab Ethylene Glycol Level 1225 NE Second Ave Portland, OR 97232.

LEGACY CS: 503-413-1234. NO TEST CODE

Processing: Do not centrifuge. Transport: Transport on ice.

Preferred Collection/Volume: Blue Cit Volume: Full tube Plasma Pediatric Requirements: x 2 Pediatric BLUE CIT Plasma

Test Schedule: 24 hrs/day Test Facility: OHSU Laboratories Hemostasis and Thrombosis, Dillehunt Hall, Rm 4040 3181 S.W. Sam Jackson Park Road Euglobulin Clot Lysis Portland, OR 97239

Specimen Stability: Frozen Test Code: OHSU TC 071-0554

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: Urine collecte in a AP99 Random Volume: 50 mL of urine Minimum Volume: 20 mL of urine

TAT: 24 Hours Extended DS, Urine Testing Schedule: Daily GC/MS Scan Method: Immunoassay Testing Location: Airport Way Laboratory

Processing: Refrigerate specimen if held overnight Transportation: Send AP99 in the Blue tote to Airport Way Laboratory

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Saturday Morning Method: Enzyme Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

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Clinical Data: Antibodies to Sm are highly specific for systemic lupus erythematosus (SLE) and, when present, are considered a marker antibody. However, these antibodies are found in only 20% of patients with SLE. RNP antibodies (also known as anti-u1 or ribonucleoprotein antibodies) are found in 45% of SLE patient, but are also observed in numerous other disease states such as Sjogren's syndrome, scleroderma, and polymyositis. Elevated levels of antibodies to RNP are seen in mixed connective tissue disease. In SLE, RNP antibodies have been associated with a relatively benign disease course with lower incidence of renal and central nervous system involvement. Patients may be considered positive for RNP antibodies when the RNP antibody result is significantly higher than the Sm antibody result.

Extractable Nuclear Patient Preparation: Fasting is required. Antigen Antibody Specimen Stability: Room Temperature: 4 days Refrigerated: 7 days Frozen: 30 days Test Code: CHANTILLY TC 4272

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: Eye or Corneal scrapings: Liquid Amies Transport swab (E- Swab). Vitreous/Aqueous Fluid: Sterile leakproof container/syringe without needle. Alternative Collection: Direct innoculation by clinician onto media. Consult with Laboratory for appropriate media types.

TAT: Routine Culture: 3 - 5 Days Cornea Culture: 10 - 14 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: If transplant cornea for sterility only, refer to Sterility Culture. Eye Culture Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired collection/transport device. Greater than 48 hours post collection (Eswab) Greater than 24 hours post collection if collected in sterile container or directly inoculated media. Dry Swab.

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: 2 Blue Cit Volume Full Tube

TAT: Please call 503-494-8445 if STAT Test Schedule: M-F 8:30am-4:30pm Method: Time-temperature dependent factor VIII assay (clotting Bethesda assay). Factor 8 Inhibitor Test Facility: OHSU Laboratories Homeostasis and Thrombosis 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Clinical Data: Assay to detect a circulating anticoagulant to factor VIII. Some patients with hemophilia A develop inhibitors to infused factor VIII, which may completely neutralize the action of the factor. In rare cases individuals without hemophilia may also develop factor VIII inhibitors. For these patients, inhibitors result in spontaneous episodes of bleeding with no prior indication of any

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coagulation disorder. The action of the inhibitors differs somewhat between patients with hemophilia A and those without the disorder. Inhibitors in hemophiliacs tend to result in complete inactivation of factor VIII when enough inhibitors are present. In contrast, patients without hemophilia who develop inhibitors may still have some factor VIII activity even though the inhibitor is present. Simple vs. complex kinetics noted.

Patient Preparation: Clinician Instructions ONLY: Avoid warfarin (Coumadin(R)) for 2 weeks and heparin for 2 days prior to test.

Transport frozen on ice. DO NOT ALLOW TO THAW. Test Code: OHSU TC 068-0573

Processing: Centrifuge immediately. Aliquot 1.0 mL plasma into 2 aliquot tubes. Freeze solid. . Transport: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection/Volume: 2 Blue Cit Volume Full Tube

TAT: Please call 503-494-8445 if STAT Test Schedule: M-F 8:30am-4:30pm Method: Clotting, Bethesda assay Test Facility: OHSU Laboratories Homeostasis and Thrombosis 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Clinical Data: Inhibitors to factor IX arise in about 3% of persons with severe hemophilia B, which is less common than the incidence of inhibitors to factor VIII in persons with severe hemophilia A. In both types of hemophilia, most inhibitors arise early in life, after only a few injections of the missing clotting factor. Inhibitors to factor IX are sometimes more dangerous than those to factor VIII. The reaction Factor 9 Inhibitor between factor VIII and its inhibitor-antibody does not cause symptoms. The reaction between factor IX and its inhibitor-antibody may cause severe allergic reactions which can be life- threatening (so-called "anaphylactic reactions"). Such reactions may occur with the very first infusion of factor IX after an inhibitor has developed - before anyone is aware that an inhibitor has arisen. All inhibitors in hemophilia B do not cause allergic reactions on exposure to more factor IX. Development of an inhibitor to factor IX can be predicted, at least in part, by determining the kind of factor IX gene mutation the baby has.

Patient Preparation: Clinician Instructions ONLY: Avoid warfarin (Coumadin(R)) therapy for 2 weeks and heparin therapy for 2 days prior to the test.

Transport frozen on ice. DO NOT ALLOW TO THAW. Test Code: OHSU TC 068- 0574

Processing: Centrifuge immediately. Aliquot 1.0 mL plasma into 2 aliquot tubes. Freeze solid. . Transport: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection: 2 BLUE CIT Note: Tube must be filled to minimum fill indicator Volume: 1.0 mL Plasma x2

TAT: Run weekly, Please call 503-494-8445 if STAT Test Schedule: Mon-Fri, 8:30am-4:30pm Method: PT based clotting method. Testing Location: OHSU-Dillahunt Hall 3181 S.W. Sam Jackson Park Road 9th Floor Hatfield Lab Portland, OR 97239 TC# 068-0548

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Clinical Data: Assay to document factor II deficiency. Two classes of autosomal recessive inherited abnormalities manifest clinically; both are uncommon. Quantitative and qualitative (dysfunctional) defects occur. Transient acquired factor II deficiency has been briefly described, associated with Mycoplasma pneumoniae infection. Rarely, hypoprothrombinemia may be associated with the presence of a lupus anticoagulant.

Patient Preparation: Clinician Instructions ONLY: Avoid warfarin (Coumadin Factor II Assay (R) therapy for 2 weeks and heparin therapy for 2 days prior to the test.

Note: Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200

Processing: Centrifuge immediately. Prepare platelet poor plasma. Aliquot 1mL plasma into 2 aliquot tubes. Freeze solid. Transport: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection/Volume: 2 BLUE CIT Pediatric Requirements: 1 Pediatric BLUE CIT Tube must be filled to minimum fill indicator

Test Schedule: M-F 8:30am-4:30pm Test Facility: OHSU - Dillahunt Hall 3181 S.W. Sam Jackson Park Rd 9th Floor Hatfield Lab Portland, OR 97239 Test Code: OHSU TC 068-0558 Specimen Stability: CRITICAL FROZEN

Clinical Data: Assay to document Factor IX deficiency. Factor IX deficiency (PTC or plasma thromboplastin component or Christmas disease) is commonly referred to as hemophilia B. It has a recessive sex-linked mode of inheritance, males are affected, females are carriers. It occurs in 1 of 25,000 males. Low levels of factor IX may be present in patients with liver disease. Severity of the symptoms correlate Factor IX Assay directly with the degree of prolongation of APTT test (level of factor IX deficiency). Mildly affected patient may show excessive bleeding only with major trauma or surgery. The APTT may not be prolonged if the factor IX level is over 25%. In most cases of hemophilia B, the prothrombin time and thrombin times are normal. A subgroup of cross reacting material positive hemophilia B patients, however, is defined by its markedly prolonged ox-brain prothrombin time. The molecular defect of this "hemophilia Bm" (factor IXHilo) has recently been described. Methodology: APTT based clotting assay.

Patient Preparation: Clinician Instructions ONLY: Avoid warfarin (Coumadin (R)) therapy for 2 weeks and heparin therapy for 2 days prior to the test

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Prepare platelet poor plasma and aliquot within two hours of collection into Referred Test aliquot tube. Freeze solid. Transport: Transport frozen on ice.

Preferred Collection/Volume: 2 BLUE CIT Tube must be filled to minimum fill indicator

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TAT: Run 1-2 times per week, Please call 503-494-8445 if STAT Test Schedule: M-F 8:30am - 4:30pm. Method: PT based clotting method Test Facility: OHSU - Dillahunt Hall 3181 SW Sam Jackson Park Rd 9th Floor Hatfield Lab Portland, OR 97239 503-494-7383 TC # 068-0560

Clinical Data: Assay to document factor V deficiency. Factor V deficiency is inherited as an autosomal recessive, males and females are equally affected. Only homozygotes have bleeding symptoms; heterozygotes are largely asymptomatic. Symptoms include ecchymoses, epistaxis, menorrhagia, and bleeding following trauma and tooth extraction. GI hemorrhage and hemarthrosis Factor V Assay may occur. Severity of bleeding does not correlate directly with factor V level and symptoms are often mild, even in homozygotes. Homozygous V deficient individuals usually have prolonged whole blood clotting time, prothrombin time, and APTT.

Patient Preparation: Clinician Instructions ONLY: Avoid warfarin (Coumadin (R)) therapy for 2 weeks and heparin therapy for 2 days prior to the test

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Centrifuge immediately. Prepare platelet poor plasma. Aliquot 1.0 mL plasma into 2 aliquot tubes. Freeze solid. Transport: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection/Volume: 5.0 mL whole blood, LV5 (EDTA) Alternative Collection: 2.0 mL Whole blood.

TAT: 2-7 Days Test Schedule: Thur Method: Invader Assay Test Facility: AWL: Molecular Diagnostics

Factor V Mutation, Clinical Data: n/a PCR Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Heparinized or frozen specimen.

Processing: Do Not centrifuge. Transport: Transport at 2-8 C. Do not freeze.

Preferred Collection: 2 Blue Cit Tube must be filled to the minimum fill indicator

TAT: Run weekly, Please call 503-494-8445 if STAT Test Schedule: Monday-Friday 8:30am-4:30pm Method: PT based clotting method Testing Location: OHSU Dillahunt Hall 3181 S.W. Sam Jackson Park Road

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Portland, OR 97239 TC# 068-0563

Clinical Data: Assay to document factor VII deficiency. Deficiency of factor VII should be considered in patients with a prolonged prothrombin time but a normal APTT. While isolated deficiency is rare, two forms exist. Both are autosomal recessive and affect both sexes. In one form the VII molecules are decreased while In the second group an abnormally formed molecule is produced. Bleeding symptoms may be severe in homozygotes and include epistaxis, ecchymoses, GI bleeding, hemarthroses, menorrhagia, and umbilical cord hemorrhage. Fatal cerebral hemorrhage may occur. Heterozygotes are usually asymptomatic. Homozygotes have prolonged prothrombin time (corrected by adding normal plasma) but normal APTT, cephalin activated clotting time, and thrombin time. Factor VII Assay Patient Preparation: Clinician Instructions ONLY: Avoid warfarin (Coumadin(R)) therapy for 2 weeks and heparin therapy for 2 days prior to the test.

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200.

Processing: Prepare platelet poor plasma and aliquot within two hours of collection into Referred Test aliquot tube using a plastic pipette. DO NOT USE FILTER. Freeze solid. Transport: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection: BLUE CIT x2 Tube must be filled to minimum fill indicator Volume: 1 mL plasma Neonates minimum volume plasma 200 µl, if not combined with other tests.

TAT: 2-7 days Test Schedule: Once per week Method: Clot based Test Facility: AWL

Factor VIII Activity Patient Preparation: If using a butterfly, a citrate discard tube must be used first, when citrate is the first tube collected.

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Process specimen as soon as possible. Prepare platelet poor plasma on site. Freeze solid. Second tube can be used for Von Willebrand Factor Antigen, if ordered. Transport: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection/Volume: 2 BLUE CIT Tube must be filled to minimum fill indicator

TAT: Please call 503-494-8445 if STAT Test Schedule: Monday-Friday 8:30am-4:30pm Method: Chromogenic Test Facility: OHSU - Dillahunt Hall 3181 SW Sam Jackson Park Rd 9th Floor Hatfield Lab Portland, OR 97239 503-494-7383

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TC # 068-5050

Clinical Data: Diagnostic Criteria: Factor X, Chromogenic Assay is indicated to monitor oral anticoagulant therapy in patients with a lupus inhibitor or thrombin inhibitor (argatroban, etc.) + coumadin. This method is not affected by the lupus inhibitor or by therapeutic heparin levels. Assay performed to document congenital and acquired factor deficiency. (Order Factor X, chromogenic if patient on argatroban, lepirudin or other thrombin inhibitors). Acquired deficiencies occur with significant hepatic dysfunction and with oral anticoagulant (coumarin) therapy. Factor X levels are most frequently used to Factor X Assay monitor oral anticoagulant therapy in patients with lupus inhibitors. Factor X deficiency may be associated with primary systemic amylodosis. The rare clinical condition (X deficiency) is inherited as autosomal, incompletely recessive. About 25 cases of hereditary factor X deficiency have been reported.

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Centrifuge immediately. Prepare platelet poor plasma. Aliquot 1.0 mL plasma into 2 aliquot tubes. Freeze solid. Transport: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection/Volume: 2 BLUE CIT Tube must be filled to minimum fill indicator

TAT: Please call 503-494-8445 if STAT Test Schedule: Monday-Friday 8:30am-4:30pm Method: APTT based clotting method. Test Facility: OHSU - Dillahunt Hall 3181 SW Sam Jackson Park Rd 9th Floor Hatfield Lab Portland, OR 97239 503-494-7383 TC # 068-0562

Clinical Data: Assay to document Factor XI deficiency. Homozygous patients have bleeding symptoms with post-trauma/post-surgical hemorrhage, epistaxis, hematuria, and menorrhagia. Excessive postpartum hemorrhage is especially Factor XI Assay common. Severity of bleeding does not always correlate with the plasma level of factor XI. This may relate in part to the mechanism of activation of factor XI. Heterozygotes are asymptomatic. Homozygotes have prolonged whole blood clotting time and APTT. Prothrombin time, thrombin time, bleeding time, platelet count, and platelet function tests are normal.

Patient Preparation: Clinician Instructions ONLY: Avoid warfarin(Coumadin) therapy for 2 weeks and heparin therapy for 2 days prior to test.

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Centrifuge immediately. Prepare platelet poor plasma. Aliquot 1.0 mL plasma into 2 aliquot tubes. Freeze solid. Transport: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection/Volume: 2 BLUE CIT Tube must be filled to minimum fill indicator

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TAT: Run weekly Test Schedule: Monday-Friday 8:30am-4:30pm Method: APTT based clotting method. Test Facility: OHSU - Dillahunt Hall 3181 SW Sam Jackson Park Rd 9th Floor Hatfield Lab Portland, OR 97239 503-494-7383 TC # 068-0561

Clinical Data: Assay to document Factor XII deficiency. Patients usually do not have bleeding symptoms as clotting system can be activated by alternate paths. Occasionally, patients suffer thromboembolic episodes that apparently relate to impaired fibrinolytic activity (one of the funcitons of XIIa is to activate plasminogen Factor XII Assay to plasmin). Homozygous XII deficient patients have prolonged whole blood clotting times and APTT results. Euglobulin clot lysis time is usually significantly prolonged. Prothrombin time, thrombin time, and bleeding time are normal in the XII deficient patient.

Patient Preparation: Clinician Instructions ONLY: Avoid warfarin therapy for 2 weeks and heparin therapy for 2 days prior to the test.

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Centrifuge immediately. Prepare platelet poor plasma. Aliquot 1.0 mL plasma into 2 aliquot tubes. Freeze solid. Transport: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection/Volume: 2 BLUE CIT Pediatric Requirements: 1 Pediatric BLUE CIT Tube must be filled to minimum fill indicator

Test Schedule: M-Th, as needed Method: Urea solubility. Test Facility: OHSU - Dillahunt Hall 3181 SW Sam Jackson Park Rd 9th Floor Hatfield Lab Portland, OR 97239 TC # 068-0551 Specimen Stability: CRITICAL FROZEN

Clinical Data: Assay to evaluate bleeding disorders due to homozygous deficiency Factor XIII Assay of factor XIII. The test is important in patients with bleeding and normal routine coagulation tests. Homozygotes have slow progressive bleeding, often hematomas, hemorrhagic cysts, bleeding after cuts and poor wound healing. Death may be due to intracranial hemorrhage. An important early sign is persistent umbilical stump hemorrhage. Cases of liver disease, pregnancy, sickle cell disease, and Henoch-Schonlein purpura may have moderately decreased factor XIII levels (without resultant bleeding). Hemorrhagic Diarrhea in Crohn's disease may in part be due to an acquired factor XIII deficiency. Levels are increased in patients with acute myocardial infarction.

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Prepare platelet poor plasma and aliquot within two hours of collection into Referred Test aliquot tube. Freeze solid.

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Transport: Transport frozen on ice.

Preferred Collection/Volume: RED 10 Volume: 10 mL Serum

Test Schedule: Performed once per week Test Facility: OHSU Laboratories Biochemical Genetics 2525 SW 3rd Avenue, Suite 350 Portland, OR 97239

Clinical Data: Precipitin panels demonstrate the presence of precipitating antibody Farmers Lung Disease (predominantly IgG and IgM) by immunodiffusion (Ouchterlony) in gel medium. Conatins all the common thermophiles, A. pullulans, Aspergillus, barn dust and avian mix among others. Most clinically significant results occur when multiple and sharp precipitin lines are found. A trace precipitin band is rarely important and a weak band is equivocal.

Test Code: OHSU TC064-0907

Processing: Do not Centrifuge Transport: Refrigerate if held.

Preferred Collection/Volume: Patient should keep sample cold until received in lab as soon as possible. DC99 (clean container) Volume: 2.0 grams Stool.

TAT: Report available: Next Day Test Schedule: Monday-Friday Evening Method: Sudan Stain Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Fecal Fat Qualitative Chantilly, VA 20153

Specimen Stability: Room Temperature: 1 hour Refrigerated: 5 days Frozen: 30 days Test Code: CHANTILLY TC 13118

Processing: Freeze solid upon receipt. Transport: Transport frozen on ice

Preferred Collection/Volume: Timed Samples: Collect sample in 1 gallon, plastic leak- proof container with screw cap Random Samples: Collect in sterile specimen container. Record total collection time (24, 48, or 72 hours) on container. Freeze sprecimen during collection. Do not submit specimen in metal paint cans, as processing poses a safety hazard. Specimens received in paint cans will be rejected. Feces from a timed (24, 48, or 72 hour) stool collection Volume: 20g Frozen Stool

TAT: 4-6 days Fecal Fat Quantitative Test Schedule: 4 days a week a.m. Method: Nuclear Magnetic Resonance (NMR) Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Excessive Fat in stool, steatorrhea, is useful in diagnosing patients with malabsorption and maldigestion, e.g., pancreatic failure. In addition, results may be useful in monitoring patients receiving exogenous enzyme therapy and chronic diarrhea.

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Patient Preparation: Patient should be on a diet including 100-150 grams of fat per day for 3 days prior to collection and during collection period. Patient should refrain from laxative use or ingest any synthetic fat substitutes (Olestra). Patient should avoid fat blocking nutritional supplements. Pediatric Patients: Do not use diaper ointments as these may interfere with test results

Specimen Stability: Room temperature: 4 days Refrigerated: 6 days Frozen: 14 days Record total collection time (24, 48, or 72 hours) and weight on test requisition and container. If entire collection is sent to the lab, the lab will weigh the sample. TC# 455

Processing: Freeze specimen during collection. Transport: frozen

Preferred Collection: Fresh stool collected in a sterile leakproof container Alternative Collection: Total fix or formalin stool transport vial.

TAT: 1 Day Testing Schedule: Daily Method: Microscopy Rejection Criteria for Fresh Stool: Fecal WBC Hard formed stool 1 hour unrefrigerated 24 hours refrigerated

Processing: Refrigerate fresh stool Transportation: Send fresh stool on ice. Send total fix or formalin stool transport vial room temperature. Transport specimen in Red Tote to Airport Way Laboratory.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: 2-3 days Test Schedule: Set up: 2 days a week a.m Method: High Performance Liquid Chromatography (HPLC) Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130 Felbamate Level Clinical Data: Felbamate is an antiepileptic drug used to treat patients with a variety of types of seizures. Therapeutic drug monitoring is useful to optimize dose and avoid toxicity.

Patient Preparation: Preferred time to collect specimen is 1 hour prior to next dose.

Specimen Stability: Room temperature: 72 hours Refrigerated: 5 days Frozen: 30 days Test Code: SJC TC 68999P

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Note if serum or plasma on specimen.

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Transport: Refrigerate if held.

Preferred Collection/Volume: SS RED Volume: 1.5mL s or p Alternative Collection: RED10 GRN Na Hep, GRN Li Hep, GRN Na Hep PST, GRN Li Hep PST

TAT: 24 Hours Ferritin Test Schedule: Mon - Sat Method: Chemiluminescent Immunoassay Test Facility: AWL

Processing: Centrifuge. Aliquot if RED 10 or GRN Na Hep or GRN Li Hep Transport: Refrigerate if held.

Preferred Collection: Adeza Specimen Collection Kit/swab

TAT: STAT Test Schedule: Daily Method: Immunoassay Test Facility: Sunnyside Medical Center and Westside Medical Center Stability: Stable at room temp. up to 8 hrs, refrigerated up to 72 hrs Fetal Fibronectin Note: Collection sites are primarily: SMC and WMC Labor and Delivery.

Processing: Send to Sunnyside or Westside Medical Center immediately. Rejection Criteria: Specimens collected by any sample device other than the Adeza Biomedical Specimen Collection kit.

Preferred Collection: Blue Cit Tube must be filled to minimum fill indicator Volume: 1mL plasma Neonates minimum volume plasma 100 µl, if not combined with other tests.

TAT: 24 hours Test Schedule: Daily Method: Clot based Test Facility: AWL, SMC, WMC

Fibrinogen Patient Preparation: If using a butterfly, a citrate discard tube must be used first, when citrate is the first tube collected.

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: If routed to INT, redirect to WMC for testing. If unable to test within 6 hours of collection, prepare platelet poor plasma. Freeze solid. Transport: Transport full tubes at room temperature. If frozen, keep frozen during transport.

Preferred Collection/Volume: Bone Marrow in Culture Tube Volume: 3mL bm Alternative Collections: Sodium heparin (royal blue-top) tube Sodium heparin lead-free (tan-top) tube 5x5 mm tumor tissue

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Lymph node in transport media Formalin fixed, paraffin embedded tissue block

TAT: 5 days Test Schedule: daily am Test Method: Fluorescence In-Situ Hybridization (FISH) Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Collection Instructions Bone marrow transport medium is available upon request. Submit a biopsy of lymphoid tissue (e.g. lymph node) in a transport medium (available upon request), or a formalin- fixed, paraffin-embedded block. FISH, ALK, 2P23 Rearrange Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order. Clinical Significance The anaplastic large cell lymphoma specific chromosomal rearrangements of 2p23 can be proved by FISH. The patients carrying t (2; 5) or ALK fusion protein have been reported to have a good response to chemotherapy and favorable outcome.

Transport Temperature Room temperature Specimen Stability Ship room temperature. Specimen viability decreases during transit. Send specimen to testing laboratory for viability determination. Do not reject. TC# 16114

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 4.0 mL Serum

TAT: Report available: 2 days Test Schedule: Set up: 2 days a week p.m Method: Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Monitoring the flecainide concentration is used to assure compliance and avoid Flecainide Level toxicity of this cardiac drug used to treat ventricular tachcardia and premature contractions.

Patient Preparation: Preferred time to collect specimen is 1 hour prior to next dose.

Specimen Stability: Room temperature: 5 days Refrigerated: 7 days Frozen: 30 days Test Code: SJC TC 5309X

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

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Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: 1-2 days Test Schedule: Sunday-Saturday Method: Quantitative Bioassay Test Facility: ARUP Laboratories 500 Chipeta Way Salt Lake City, UT 84108-1221

Flucytosine Level Patient Preparation: Include time of last dose, and list of all antibiotics that the patient is receiving or has received in the past 48 hours.

Specimen Stability: Room Temperature: 2 hours Refrigerated: 24 hours Frozen: 1 week Test Code: ARUP TC 0060846

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Freeze solid. Transport: Transport frozen on ice

Preferred Collection/Volume: Random urine in AP99 Volume: 20.0 mL Urine

TAT: Reports in 4 days . Test Schedule: Tuesday & Thursday; Afternoon Method: Ion specific Electrode Test Facility: Quest Diagnostics Chantilly Nichols Institute 14225 Newbrook Dr. Fluoride Urine Chantilly, VA 20151-2228

Specimen Stability: Room temperature: 72 hours Refrigerated: 7 Days Frozen: 28 Days Test Code: CHANTILLY TC 10207

Transport: Transport on ice.

Preferred Collection/Volume: RED 10 Volume: 1.5 mL s Alternative Collection: GRN Li Hep, GRN Na Hep Volume 1.0 mL p

TAT: 24 Hours Folate Level Test Schedule: Mon - Sat Method: Chemiluminescent Immunoassay Test Facility: AWL

Processing: Centrifuge, Aliquot Transport: Refrigerate if held. Freeze after 48 hrs.

Preferred Collection/Volume: RED 10 collected by venipuncture Volume: 4.0 mL s Absolute Minimum Volume: 2.0 mL s ***Volume is critical for testing***

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TAT: 24 Hours Test Schedule: Mon - Fri Method: Fluorescence Immunoassay Test Facility: AWL Food Allergen Panel Test includes the following allergens: Milk, Egg White, Wheat, Soybean, Peanut, Shrimp, Codfish

Processing: Centrifuge, aliquot Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL Whole blood

Test Facility: Kaiser Regional Molecular Genetics Lab 5755 Cottle Rd. Bldg #6 San Jose, CA 95123 (408)972-3308. Fragile X (FRAXA) Molecular Refrigerate if held overnight. Ship Room Temperature

Processing: Do not Centrifuge. Transport: Send Room Temperature

Preferred Collection/Volume: RED SST Volume: 1.5 mL s Alternative Collection: RED 10 GRN Li Hep PST Absolute Minimum Volume: 1.0 mL s/p

TAT: 24 Hours Free T4 Test Schedule: Mon - Sat Method: Chemiluminescent Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot RED 10. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL Whole blood

Test Facility: Kaiser Regional Molecular Genetics Lab 5755 Cottle Rd. Bldg #6 San Jose, CA 95123 (408)972-3308. Friedreich Ataxia Mutation Analysis Refrigerate if held overnight. Ship Room Temperature

Processing: Do not Centrifuge. Transport: Send Room Temperature

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Next day. Test Schedule: Monday-Friday; Morning, Afternoon and Night

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Method: Colorimetric Test Facility: Quest Diagnostics Chantilly Nichols Institute 14225 Newbrook Dr. Chantilly, VA 20151-2228

Clinical Data: Fructosamine reflects intermediate glycemic control (over 1-2 weeks). Testing is often used to complement glucose (immediate glycemic control) and hemoglobin A1c testing (long- term glycemic control).

Samples that have hemolysis, or moderate or gross icterus are unacceptable. Specimen Stability: Fructosamine Serum __Room temperature: 7 Days __Refrigerated: 14 Days __Frozen: 30 Days Plasma __Room temperature: 72 Hours __Refrigerated: 14 Days __Frozen: 30 Days Test Code: CHATNILLY TC 8340

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 1.5 mL s Alternative Collection: GRN Li Hep 4 Volume: 1.0 mL p

TAT: 24 Hours FSH Level Test Schedule: Mon - Sat Method: Chemiluminescent Immunoassay Test Facility: AWL

Processing: Centrifuge and Aliquot Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 2 - 4 days Test Schedule: Monday-Friday Method: Electrochemiluminescent (ECL) Test Facility: Esoterix FSH Pediatric 4301 Lost Hills Road Calabasas Hills, CA 91301

Test Code: ESOTERIX TC 500192

Processing: Centrifuge and aliquot into Referred Tests aliquot tube within one hour of collection. Transport: Transport on ice.

Preferred Collection/Volume: RED 10 Alternate Collection: REDSST Volume: 5.0 mL s Absolute Minimum Volume: 3mL s

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TAT: Results released on day of test Test Schedule: Tuesdays Method: Indirect Fluorescent Antibody (IFA) Stain Test Facility: Public Health Division 800 NE Oregon Street Portland, OR 97232

Clinical Data: The FTA test is recommended for follow-up of reactive non-treponemal tests for syphilis (e.g. the RPR), and as a single test in patients suspected of late syphilis. Inconclusive final FTA-ABS reports indicate the initial specimen submitted has been tested twice and cannot be interpreted as either reactive or nonreactive. If it is the second specimen submitted on a patient and the report is again inconclusive, it is impossible to state definitively that the patient does or does not have syphilitic infection.

Specimen Stability: Refrigerated: 7 days NO TEST CODE

Processing: Centrifuge ASAP and aliquot into Referred Tests aliquot tube. DO NOT FREEZE Transport: Transport on ice.

Preferred Collection/Volume: Venous blood draw place in SPS or isolator tube/must be completely filled Alternative Collection: n/a

TAT: 3 - 6 Weeks Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: n/a Patient History: n/a Fungus Culture, Blood Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Greater than 24 hours post collection Expired collection/transport device SPS or isolator tube not completely filled

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: Hair: Collect 10-12 affected hairs with the base of the shaft intact. Skin: Skin scrapings at the active margin of the lesion. Nail: Clip a generous portion of the affected area and collect material from under the nail. Submit in sterile leakproof container Alternative Collection: Direct innoculation by clinician onto DTM and IMA media. Embed specimen gently into the media.

Fungus Culture, TAT: 3 - 6 Weeks Hair/Skin/Nails Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: n/a Patient History: n/a

Patient Preparation: n/a

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Labeling: If media is directly inoculated by clinician, place patient label on the back of the slant

Rejection Criteria: Greater than 5 days post collection Additive in a sterile container (i.e. Formalin or alcohol) Directly inoculated media that is expired or dehydrated

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: BAL or Sputum in sterile leakproof container / >=1mL. Body Fluids in sterile leakproof container / >=1mL. Tissue in in sterile leakproof container with 1 mL of sterile saline added / >=1m. Bone Marrow in SPS yellow top tube/completely filled. Urine in sterile leakproof container / 5 mL. Eye/Mouth/Vaginal/other: Liquid Amies Transport Swab (E-Swab). Alternative Collection: n/a

TAT: 1 - 6 Weeks Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: If source is blood or hair/skin/nails, refer to Fungus Culture, Blood or Fungus Culture, Hair/Skin/Nails Fungus Culture, Other Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired collection/transport device Greater than 5 days post collection Dry Swab SPS tube not completely filled Dehydrated specimens Sterile body fluid received on a swab

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p Alternative Collection: RED SST or serum acceptable Absolute Minimum Volume: 0.2mL

Gamma Glutamyl TAT: 24 Hours Tranferase Test Schedule: Daily Method: Photometric Rate Test Facility: AWL

Processing: Cent. Refrigerate if held

Preferred Collection/Volume: COLLECT MON-FRI BEFORE 1100 ONLY!!! Use Quantiferon Gold Testing Kit for collection. Draw 1ml of blood into each of the 3 specimen tubes. Shake each tube vigorously for 5 seconds. Assure that the state Microbiology Request Form has been completely filled in with the following information: Patient Name Date of Birth County of Residence Date of Collection Time of Collection

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Gender Specimen is Blood Test is Quantiferon Testing Submitter Code X20007 Volume: 1.0 ml into each of the three tubes provided in collection kit. 1.0 ml line is printed on tube label.

TAT: 2 to 4 days Test Schedule: Monday-Friday Method: Interferon-Gamma detection and quantification by ELISA in response to TB antigens Test Facility: Oregon State Public Health Lab 3150 NW 229th Ave. STE 100 Hillsboro, OR 97124 Gamma Interferon (TB) Salem and Longview Kelso locations are unable to process requests for this test. Samples collected from these locations are unable to be transported within the required time to the state lab for testing. Patients requiring this test should present to the laboratory Monday-Friday before 1100 am for collection.

Rejection Criteria: Unlabeled specimens, specimens not submitted as per collection instructions or without completed requisition forms will not be processed

Processing: Place all three labeled samples and completed Microbiology Request form into bag provided with kit. Keep at room temperature. Do not centrifuge . Send specimens and form with next courier. CORE LAB: Deliver to Referred Tests department STAT. Transport: Room Temperature

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Report available: 16 Days Test Schedule: Monday, Wednesday Morning Method: Enzyme-Linked Immunosorbent Assay Test Facility: Athena Diagnostics, Inc. Four Biotech Park 377 Plantation Street Worcester, MA 01605 Ganglioside GM1B Antibody Clinical Data: Detection of anti-GM1, anti-asialo-GM1 and anti-GD1b antibodies using covalent ELISA technology. Type of Disorder: Peripheral Neuropathy Typical Presentation: Slowly progressive, distal motor neuropathy

Specimen Stability: Room Temperature: 3 days Refrigerated: 2 weeks Frozen: not established Test Code: CHANTILLY TC 118512 TO ATHENA TC 270

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

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TAT: Report available: Next Day Test Schedule: Monday-Friday Evening Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Patient Preparation: Overnight fasting for a minimum of 12 hours is required. Gastrin Level Specimen Stability: Room Temperature: Unacceptable Refrigerated: Unacceptable Frozen: 28 days (-20c) Test Code: CHANTILLY TC 478

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Freeze. Transport: Transport frozen on ice

Preferred Collection/Volume: Vaginal or cervical specimen: Liquid Amies Transport swab (E-Swab) Alternative Collection: n/a

TAT: 2 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: If looking for Trichomonas, yeast or Bacterial Vaginosis (BV) order a T+Y Wet Mount. If looking for Neisseria gonorrhoeae , order a Gonorrhea Culture. If looking for GC/Chlamydia refer to CT/NG molecular test. If specimen is an abscess or lesion, refer to wound or abscess/aspirate culture. Genital Culture For vaginal specimens only. This culture will look for aerobic bacteria and Candida species that are in clinically significant amounts. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired collection/transport device Greater than 24 hours post collection Dry Swab

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: Green 3 Sodium Heparin Volume: 3mL WB MINIMUM VOLUME 1mL WB **No Gel Barrier Tubes**

TAT: 14-21 days Test Schedule: Mon-Fri Genomic Array Assay Method: Single nucleotide polymorphism (SNP) microarray analysis. Test Facility: AWL

Clinical Data: Include clinical indication. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

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Processing: ** No Gel barrier tubes ** Do not Centrifuge. Hold at Room temperature. Do NOT reject centrifuged specimen; contact Cytogenetics department at 503-258-6723 or Tie line 35x6723 Transport: Room Temperature.

Preferred Collection: GRN Li Hep Alternative Collection: RED 10 LAV EDTA GRN Na Hep Volume: 0.5 mL p lasma or serum ( Absolute Minimum Volume: 0.2 mL plasma or serum) Note: No gel barrier tubes. Gentamicin Level TAT: Stat: 1 Hour Routine: 24 Hours Testing Schedule: Daily Method: Two Point Rate Testing Location: Sunnyside Medical Center and Westside Medical Center

Processing: Centrifuge specimen and aliquot, refrigerate. Transport: Send specimen to appropriate Medical Center for testing. Note: City Sprint STATs directly to appropriate Medical Center for testing.

Preferred Collection: DkGRN Li Hep Alternative Collection: RED 10 LAV EDTA DkGRN Na Hep Volume: 0.5 mL plasma or serum (Absolute Minimum Volume: 0.2 mL plasma or serum) NOTE: NO gel barrier tubes

TAT: Stat: 1 Hour Routine: 24 Hours Gentamicin Level Peak Test Schedule: Daily Method: Two Point Rate Test Facility: Sunnyside Medical Center and Westside Medical Center

Preferred draw time: IV: Draw 30 min after end of 30 min infusion or 15 min after 45-60 minute infusion. IM: Draw 60 min post injection.

Processing: Centrifuge specimen and aliquot, refrigerate if held. Transport: Send specimen to appropriate Medical Center for testing. Note: City Sprint STATs directly to appropriate Medical Center for testing.

Preferred Collection: DkGRN Li Hep Alternative Collection: RED 10 LAV EDTA DkGRN Na Hep Volume: 0.5 mL plasma or serum (Absolute Minimum Volume: 0.2 mL plasma or

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serum) NOTE: NO gel barrier tubes

TAT: Stat: 1 Hour Routine: 24 Hours Test Schedule: Daily Method: Two Point Rate Gentamicin Level Test Facility: Sunnyside Medical Center and Westside Medical Center Trough Note: Preferred draw time is trough, immediately before next dose.

Processing: Centrifuge specimen and aliquot, refrigerate if held. Transport: Send specimen to appropriate Medical Center for testing. Note: City Sprint STATs directly to appropriate Medical Center for testing.

Preferred Collection: Stool collected and placed in the Total-Fix vial to the fill line Alternative Collection: Stool collected and placed in a Pink Para vial (10% formalin) to the fill line

TAT: 24 Hours Test Schedule: Mon - Fri Method: Enzyme Immunoassay Testing Location: AWL Giardia Antigen Recomended collection is 2 stool specimens over a 7 day period. Also acceptable: Rectal swab Submit in clean plastic container without placing in fixative, if within 48 hours of collection. Note: If greater than 48 hours, place swab in parasitology fixative.

Processing: Specimens in Parasitology Fixitive stored at room temp. Note: Untreated swabs need to be refrigerated

Preferred Collection: RED SST Volume: 1.0 mL serum Alternative Collection: RED 10 Minimum Volume: 0.2 mL serum

TAT: 1-3 Days Gliadin IgG and IgA Testing Schedule: Monday -Friday Method: Mutiplex Immunoassay Testing Location: Airportway Laboratory

Note: Includes Deaminated Gliadin Peptide IgA and IgG.

Processing: Centrifuge, aliquot (if collected in a RED 10), refrigerate.

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Preferred Collection/Volume: 2 LAV EDTA EDTA plasma only Volume: 3.0 mL Plasma

TAT: Report available: 5 days Test Schedule: Tuesday,and Friday; a.m Method: Extraction • Radioimmunoassay (RIA) Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Glucagon measurement is useful primarily when considering a glucagon- Glucagon Level secreting tumor of the pancreas. Glucagon is also used to diagnose glucagon deficiency in patients with hypoglycemia.

Patient Preparation: Overnight fasting is required

Specimen Stability: Room temperature: 24 hours Refrigerated: 24 hours Frozen: 28 days Test Code: CHANTILLY TC 519 TO SJC TC 519

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Freeze. Transport: Transport frozen on ice

Preferred Collection: GRN Li Hep PST Alternative Collection: Red SST or Serum is acceptable Volume: 1.0 mL Minimum Volume: 0.5 mL

STAT TAT: 1 Hour TAT: 24 Hours

Glucose 1 Hour Post 50g Test Schedule: Daily

Note: See Work Instruction 2.2-08-205, GTT Preparation and Collection, before proceeding with test.

Specimen Stability: 7 days refrigerated if separated from cellular material Rejection Criteria: Samples drawn >15 min post scheduled collection time. Note: Hemolysis should be avoided

Processing: Centrifuge within 1 hour of specimen collection Transport: Refrigerated

Preferred Collection/Volume: 24 hr Urine (10g Boric Acid) Volume: 4.0mL u Absolute Minimum Volume: 1.0mL u

TAT: 24 Hours Test Schedule: Daily Method: Photometric, Hexokinase

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Test Facility: AWL

Other Acceptable Preserv: no preservative, refrigeration is required Glucose 24 Hour Urine Processing: Record collection interval hrs. Measure and record total vol. in ARE in LIS. Aliquot to 12x75 tube. Transport: Refrigerate if held.

Preferred Collection/Volume: GRN Li Hep 4 Volume: 4.0mL bf Alternative Collection: RED 10 Absolute Minimum Volume: 1.0mL bf

TAT: Stat: 1 Hour Routine: 24 Hours Glucose Body Fluid Test Schedule: Daily Method: Photometric, Hexokinase Test Facility: AWL, SMC

Use separate LIS requests if both blood and body fluids are ordered.

Transport: Refrigerate if held

Preferred Collection/Volume: CSF submitted in sterile tube Volume: 1.0 mL Absolute Minimum Volume: 0.5 mL

TAT: STAT: 1 Hour/ Routine: 24hours Test Schedule: Daily Method: Photometric, Hexokinase Test Facility: Sunnyside Medical Center Glucose CSF Westside Medical Center Interstate Medical Office

Handle and process all CSF as STAT

Processing: Use tube 4 for testing unless otherwise indicated by clinician. Transport: Transport to testing site immediately. Testing locations are Sunnyside and Westside Medical Centers and Interstate Medical Office.

Preferred Collection/Volume: GRN Li Hep PST Alternative Collection: RED SST or serum is acceptable Volume: 0.5mL p Minimum Volume: 0.2mL p/s Glucose Fasting STAT TAT: 1 Hour TAT: 24 Hours Test Schedule: Daily Note: Add on stability is 24 hours

Note: Patient must be fasting 8 hours

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Note: Use panel NFG for fasting glucose at Skilled Nursing Facilities (SNF) or by Home Health. Whole blood from unspun GRN Li Hep PST acceptable when laboratory staff is using the ISTAT to perform testing.

Specimen Stability: 7 days refrigerated if separated from cellular material Note: Hemolysis should be avoided

Processing: Centrifuge within 1 hour of specimen collection Transport: Refrigerated

Preferred Collection/Volume: Grey Na Fluor Volume: 0.5ml Absolute Minimum Volume: 0.2ml

TAT: 24 Hours Test Schedule: Daily Glucose Fasting SNF Method: Photometric, Hexokinase Test Facility: AWL

FOR Skilled Nursing Facilities/Home Health ONLY AWL: Cent, Pour

Transport: Refrigerate if held

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p Alternative Collection: RED SST or serum acceptable Absolute Minimum Volume: 0.2mL p/s

TAT: Stat: 1 Hour Routine: 24 Hours Test Schedule: Daily Method: Photometric, Hexokinase Glucose Random Test Facility: All: SMC, AWL, INT, BVT, CAS, LVK, SKY, NLR, RKW, SAL, VAN, DIV, SST, TUA, ORC

Add-on testing stability: 24 hrs iSTAT Testing Performed by LAB Staff: Whole blood from unspun GNS5 acceptable for testing

Processing: Centrifuge Transport: Refrigerate if held.

Preferred Collection/Volume: Grey Na Fluor Volume: 0.5mL Absolute Minimum Volume: 0.2mL

TAT: 24 Hours Test Schedule: Daily Method: Photometric, Hexokinase Glucose Random SNF Test Facility: AWL

Use for random glucose orders from Skilled Nursing Facilities or Home Health

Processing: Cent. Pour. Transport: LAB CENT: Refrigerate if held

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Preferred Collection: GRN Li Hep PST Alternative Collection: Red SST or Serum is acceptable Volume: 1.0 mL Minimum Volume: 0.5 mL

STAT: 1 Hour TAT: 24 Hours Test Schedule: Daily

Glucose Tolerance 2 Hr Note: Patient must be fasting 8 hours Abbreviated Pediatric Patients: Results of fasting blood sugar (FBS) are not required to administer Glucola and proceed with test. Draw fasting glucose, give pediatric patient glucola based upon weight, see Work Instruction 2.2-08-205, GTT Preparation and collection, before proceeding with test.

Specimen Stability: 7 days if separated from cellular material Rejection Criteria: Samples drawn > 15 min post scheduled collection time. Note: Hemolysis should be avoided

Processing: Centrifuge sample within 1 hour of specimen collection. Transport: Refrigerated

Preferred Collection: GRN Li Hep PST Alternative Collection: Red SST or Serum is acceptable Volume: 1.0 mL Minimum Volume: 0.5 mL

STAT TAT: 1 Hour TAT: 24 Hours Glucose Tolerance 3 Hour Test Test Schedule: Daily

Note: Patient must be fasting 8 hours

Note: For Prenatal patients only. See Work Instruction 2.2-08-205, GTT Preparation and Collection, before proceeding with test.

Specimen Stability: 7 days refrigerated if separated from cellular material Rejection Criteria: Samples drawn >15 min post scheduled collection time. Note: Hemolysis should be avoided

Processing: Centrifuge within 1 hour of specimen collection

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Transport: Refrigerated

Preferred Collection: GRN Li Hep PST Alternative Collection: Red SST or Serum is acceptable Volume: 1.0 mL Minimum Volume: 0.5 mL

STAT TAT: 1 Hour TAT: 24 Hours Test Schedule: Daily Glucose Tolerance Test, Note: Patient must be fasting 8 hours 2 HR, Gestational Diabetes CHR STUDY PRENATAL PATIENTS ONLY

See Work Instruction 2.2-08-205, GTT Preparation and Collection, before proceeding with test.

Specimen Stability: 7 days refrigerated if separated from cellular material Rejection Criteria: Samples drawn >15 min post scheduled collection time. Note: Hemolysis should be avoided

Processing: Centrifuge within 1 hour of specimen collection Transport: Refrigerated

Preferred Collection/Volume: GRN Li Hep PST Volume: 1.0 mL Alternative Collection: RED SST or serum acceptable Absolute Minimum Volume: 0.5 mL p/s

TAT: Stat: 1 Hour Routine: 24 Hours Glucose Tolerance Test, Test Schedule: Daily Each Addl Beyond 3 Method: Photometric, Hexokinase Specimens Test Facility: CAS, INT, DIV, SMC, LVK, BVT, NLR, ORC, RKW, SAL, SKY, SST, TUA, VAN

Used in conjunction with abbreviated 2 hr GTT,(G2B) or 3 hr Prenatal GTT (TT2). Use this when a patient becomes ill during a GTT and it is necessary to add on a glucose or if a physcian orders an additional glucose

Processing: Cent. within 1 hr. Refrigerate until GTT is complete

Preferred Collection/Volume: 1 LAV EDTA (Whole Blood) Volume: 1.0 mL Whole Blood

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning

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Method: Kinetic Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: G-6-PD is the most common enzyme deficiency in the world. Newborns with G- 6-PD may have prolonged and more pronounced neonatal jaundice than other newborns. Older individuals are subject to hemolytic anemia that can be induced by some foods, drugs, and infections. Glucose-6-PD Quantitative Specimen Stability: Room Temperature: 2 days Refrigerated: 7 days Frozen: Unacceptable Test Code: CHANTILLY TC 500

Processing: Do not centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Reports in 2 days. Test Schedule: Sets up 3 days a week. Method: Radiobinding Assay Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Glutamic Acid Decarboxylase (GAD65) Antibody is useful to diagnose insulin Glutamate dependent diabetes mellitus (IDDM, Type I diabetes), to assess risk for development of IDDM, to Decarboxylase 65 predict onset of IDDM, and risk of development of related endocrine disorders, e.g., thyroiditis. Antibody Before clinical onset, Type I diabetes is characterized by lymphocytic infiltration of the islet cells, and by circulating autoantibodies against a variety of islet cell antigens, including GAD-65, IA-2 (a phosphatase-like protein), and insulin (IAA).

Specimen Stability: Room temperature: 8 Hours Refrigerated: 7 Days Frozen: 6 Months Test Code: SJC TC 34878

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes. Transport: Refrigerate if held.

Preferred Collection/Volume: Liquid Amies Transport swab (E-Swab) Alternative Collection:

TAT: 2 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL Gonorrhea Culture Clinical Data: This test is for Neisseria gonorrhoeae isolation only. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired collection/transport device

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Greater than 24 hours post collection Dry Swab Improper collection/transport of media plate

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: Fluids/Tissue: Submit in sterile leakproof container. For Tissue, add a small amount of sterile saline to keep tissue moist. Swab: Liquid Amies Transport Swab (E-swab).

TAT: 1 Day Test Schedule: Daily Method: Stain Test Facility: AWL

Gram Stain Clinical Data: Orderable is for Gram Stain only. Note that Gram Stain is included with all pertinent cultures.

Rejection Criteria: Expired collection/transport device Greater than 24 hours post collection Dry Swab

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: LIM broth from Molecular testing Alternative Collection: n/a

TAT: 2 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Group B Strep Culture Clinical Data: Reflex from Molecular Test - STREP B,OB,DNA,PCR,REFLEX Patient History: Penicillin allergic status

Patient Preparation: n/a Labeling: n/a

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: RED 10 Volume: 1.0 mL s Absolute Minimum Volume: 0.5 mL s

TAT: 7 Days Test Schedule: Fri Growth Hormone Method: Chemiluminescent Immunoassay Test Facility: AWL

Processing: Cent, aliquot. Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

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TAT: 3 - 16 days Test Schedule: Tuesday Morning Method: Ligand binding assay Test Facility: Esoterix 4301 Lost Hills Road Calabasas Hills, CA 91301

Growth Hormone Specimen Stability: Binding Protein Room Temperature: 8 Hours (GHBP) Refrigerated: 24 Hours Frozen: 30 Days Test Code: CHANTILLY TC 70752 TO ESOTERIX TC 500209

Processing: Centrifuge and aliquot into Referred Tests aliquot tubes within one hour of collection. Freeze. Transport: Transport frozen on ice

Collection Instructions Draw baseline growth hormone. Patient to consume 75g glucose solution. Draw post 2 hour growth hormone level. Preferred Collection/Volume: RED 10 Growth Hormone Volume: 1 mL s Supression (2 Absolute Minimum: .5mL s Specimens) Specimen Handling Processing: Centrifuge and aliquot. Freeze. Transport: Transport frozen on ice.

Preferred Collection: GRN Li Hep PST Alternative Collection: Red SST or Serum is acceptable Volume: 1.0 mL Minimum Volume: 0.5 mL

STAT TAT: 1 Hour GTT, 2 HR, Opt Out Option TAT: 24 Hours

Test Schedule: Daily

Patient must be fasting 8 hours

CHR STUDY PRENATAL PATIENTS ONLY

See Work Instruction 2.2-08-205, GTT Preparation and Collection, before proceeding with test.

Specimen Stability: 7 days refrigerated if separated from cellular material

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Rejection Criteria: Samples drawn >15 min post scheduled collection time. Note: Hemolysis should be avoided

Processing: Centrifuge within 1 hour of specimen collection Transport: Refrigerated

Preferred Collection: RED 10 Volume: 1.0 mL Serum Minimum Volume: 0.2 mL Serum

TAT: Report available within 3 days Test Schedule: Set is on Monday, Wednesday, and Friday AM Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Haemophilus Influenzae B Antibody Specimen Stability Room temperature: 7days Refrigerated: 14 days Frozen: 30 days TC# 35135 Rejection Criteria: Gross hemolysis, gross lipemia, grossly icteric

Processing: Centrifuge and aliquot into Referred Tests aliquot tube Transport: Refrigerated

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Urine Not Acceptable Alternative Collection: CSF in Sterile Container, leak-proof Volume: 1.0 mL CSF

TAT: Report available: Next Day Test Schedule: Monday-Sunday Afternoon, Evening, Night Method: Agglutination Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Haemophilus Influenzae B Antigen Clinical Data: Meningitis is often caused by Haemophilus influenzae (Type B). Results from bacterial antigen testing should be considered presumptive and culture with Gram stain must also be performed.

Specimen Stability: Room Temperature: Unacceptable Refrigerated: 48 hours Frozen: 7 days Test Code: CHANTILLY TC 45058

Processing: Centrifuge and aliquot blood samples into Referred Tests aliquot tubes. Freeze serum and CSF. Transport: Transport frozen on ice

Preferred Collection/Volume: Collect sample 11-17 hours after last dose.

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RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: 4 Days Test Schedule: Tuesday, Thursday, Sunday Night Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Monitoring the haloperidol concentration is used to ensure compliance and to Haloperidol Level avoid toxicity of this antipsychotic drug. Haloperidol is used to treat psychotic disorders, control tics and vocal utterances associated with Giles de la Tourette syndrome, and combative, explosive hyperexcitability in children.

Patient Preparation: Specimen should be collected 11-17 hours after last dose.

Specimen Stability: Room Temperature: 2 days Refrigerated: 1 week Frozen: 1 month Test Code: CHANTILLY TC 564 TO SJC TC 564

Processing: Centrifuge and aliquot blood samples into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED SST Volume: 0.5mL s Alternative Collection: RED 10 Absolute Minimum Volume: 0.2mL s

TAT: 24 Hours Haptoglobin Test Schedule: Mon - Sat Method: Turbidometry Test Facility: AWL

Processing: Centrifuge Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 2.0 mL s Absolute Minimum Volume: 1.0 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Method: Immunoassay HBsAg Prenatal Test Facility: AWL

Add on stability: 3 days Hep testing for OB screen only

Processing: Centrifuge, Pour Transport: Refrigerate if held.

Preferred Collection: Random urine collected in a AP99 or a clean clean container

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Volume: 2.0 mL

TAT: Stats within 1 hour, Routine within 1 day Testing Schedule: Daily Method: Immuno-assay (rapid screen) Testing Location: Medical Offices Specimen Stability: HCG Urine, Self Room Temperature: 24 hours Referred Refrigerated: 48 hours The first morning specimen is preferred as it contains a higher concentration of the pregnancy hormone.

Patient Preparation: This test is used when a patient requests urine pregnancy testing. Laboraotry policy is that patient is 18 years or older. Refer younger patients to pediatrics. Test is performed within 1 hour. Patient will wait for results or return within 24 hours to pick them up. Results will be available to patient on kp.org if signed up.

Preferred Collection/Volume: x 2 Dark Blue EDTA (Whole Blood) Volume: 12.0 mL Whole Blood

TAT: Report available: 3 Days Test Schedule: Monday, Wednesday, Friday Afternoon Method: Inductively Coupled Plasma-Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Children are especially susceptible to neurologic damage from lead and acute neurologic toxicity may develop without previous symptoms.

Heavy Metal Screen Patient Preparation: Patients should refrain from seafood, antacids, vitamins with mineral supplements, and herbal preparations at least 3 days prior to specimen collection. (Refer Patient to Clinician to determine if safe to refrain from dietary supplements) Includes quantitative arsenic, lead, and mercury. .

Specimen Stability: Room Temperature: 5 Days Refrigerated: 7 Days Frozen: Unacceptable Test Code: CHANTILLY TC 5991

Processing: Do not centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume: 24 Urine in Acid Washed Container refrigerated during collection. Volume 7.0 mL urine

TAT: Report available: 3 days Test Schedule: Monday, Wednesday, Friday Afternoon Method: Inductively-Coupled Plasma/Mass Spectrometry (ICP/MS) Heavy Metal Screen Test Facility: Quest Diagnostics Nichols Institute Urine 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Excessive exposure to Heavy Metals can cause acute and chronic toxicity. Heavy Metals Panel is intended to evaluate and monitor exposure to heavy metals and evaluate the process of detoxification.

Patient Preparation: Patient should refrain from eating shellfish prior to and during specimen

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collection

Specimen Stability: Room temperature: 48 hours Refrigerated: 5 days Frozen: 14 days Test Code: CHANTILLY TC 14479

Processing: Mix sample well and aliquot into a plastic, acid-washed, metal-free leak proof container. Note on sample total volume and preservative used during collection. Transport: Tranport on ice.

Preferred Collection/Volume: 3 – 5 mL EDTA (LV3, LV5, microtainer) Alternative Collection: n/a

TAT: 2 – 3 working days Test Schedule: Monday – Friday Test Method: Supra-vital Stain Test Facility: AWL, Wet/Dry Microscopy

Heinz Body Stain Clinical Data: n/a Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: n/a Transport: Transport to AWL within 4 hrs of draw

Preferred Collection/Volume: RED SST Volume: 2.0 mL Alternative Collection: LAV EDTA Absolute Minimum Volume: 0.5 mL

TAT: 3 Days Test Schedule: Mon, Thur Helicobacter Pylori Method: Chemiluminescent Immunoassay Antibody IgG Test Facility: AWL

Stable refrigerated 3 days. Aliquot and freeze for longer periods of storage

Processing: Centrifuge Transport: Refrigerate if held.

Preferred Collection/Volume: S emisolid stool or solid stool collected in leakproof container. Do not place stool in preservative, transport medium, or swab. Watery, diarrheal stool is not acceptable. Volume: .5mL s

TAT: 4 days Test Schedule: Monday, Wednesday, Friday pm's Test Method: Immunoassay (IA) Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

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This test is intended to aid in the diagnosis of H.pylori infection and to aid in monitoring the efficacy of antimicrobial therapy. A result of "Detected" indicates H. pylori antigen is present. "Not Detected" indicates the absence of H. pylori antigens or the level of antigen is below the sensitivity of the assay.

Antimicrobials, proton pump inhibitors, and bismuth preparations are known to suppress H. pylori , and ingestion of these prior to H. pylori diagnostic testing may give a false negative result. If clinically indicated, the test may be repeated on a new specimen obtained two weeks after discontinuing treatment. Clinical Significance Colonization with H. pylori is associated with increased risk of patients developing Helicobacter Pylori gastritis, peptic ulcer disease, and gastric adenocarcinoma. Stool antigen testing provides a Antigen sensitive measure of infection including during and after treatment.

Specimen Stability Room temperature: Unacceptable Refrigerated: 72 hours Frozen: 30 days TC# 34838

Processing: Freeze sample upon receipt Transportation: Transport frozen on ice

Preferred Collection: LAV EDTA Alternative Collection: LAV EDTA Microtainer or 3 micorhematocrit capillary tubes Minimum Volume: 1 mL

TAT: 24 hours for routine specimens Testing Method: 5-Part diff Sysmex Hematology cell counter Stability:

{ Specimen is stable for 24 hrs at room temperature for all parameters.

{ Refrigerated specimens are stable for 72 hours for hemogram (ABC) Hematocrit parameters. { Specimens 48-72 hrs old require evaluation of all abnormal differentials.

Rejection Criteria:

{ Clotted specimens

{ Hemolyzed specimens

{ IV contaminated specimens

Note: Test on site whenever possible if specimen is collected in a microtainer.

Processing: Handle microtainers ASAP and test on-site when possible. Transport: Transport specimens to Airport Way Laboratory.

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Preferred Collection/Volume: 1.0 mL fluid in EDTA Alternative Collection: Heparin

TAT: 4 hours Test Schedule: Daily Hematocrit Body Fluid Method: Micro-hematocrit centrifuge Test Facility: Med offices, SMC, AWL

Preparation: Clinician collection

Processing: Stable for 4 hours

Preferred Collection/Volume: 3 microhematocrit capillary tubes Alternative Collection: 1 mL EDTA whole blood

TAT: stat 1 hour; routine same day Test Schedule: Any day Test Method: Microhematocrit centrifuge Hematocrit Spun Test Facility: Any KPNW laboratory with hematology testing

Clinical Data: This test is usually ordered on newborns

Processing: Handle microtainer ASAP and test on-site when possible Transport: Transport at refrigerator temperature when sending to AWL.

Preferred Collection/Volume: Lithium Heparin syringe Volume: 1.0 mL wb Alternative Collection: GRN Li Hep 4 Absolute Minimum Volume: 0.2 mL wb

TAT: 1 Hour Test Schedule: Daily Hematocrit, Blood Gas Method: Oximetry Test Facility: SMC

Panel for ICU testing at SMC

Processing: Do not centrifuge. Needle must be removed and syringe capped, analyze within 60 minutes of collection. Transport: Send room temp

Preferred Collection/Volume: LAV EDTA Volume: 1.0 mL whole blood

TAT: 7-10 days Test Schedule: Weekly Method: HLA-H PCR Test Facility: OHSU, Molecular Diagnostic Center Hemochromatosis DNA 2525 SW 3rd Avenue, Suite 350 Test (503) 494-5400

Clinical Data: Direct DNA-based detection by PCR of a common mutation in the HLA-H gene that causes hereditary hemochromatosis, a common iron overload disorder. C282Y heterozygotes are reflexed to H63D for additional charge. Call the lab at 503-494-5400 for more information.

Specimen Stability: Room Temperature. Refrigerate if held overnight Test Code: OHSU TC 102-8951

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Processing: Do not Centrifuge Transport: Refrigerate if held.

Preferred Collection/Volume: Lithium Heparin syringe Volume: 1.0 mL wb Alternative Collection: GRN Li Hep 4 Absolute Minimum Volume: 0.2 mL wb

TAT: 1 Hour Test Schedule: Daily Hemoglobin (Blood Gas Method: Oximetry Specimen) Test Facility: SMC

Panel for ICU testing at SMC

Processing: Do not centrifuge. Needle must be removed and syringe capped, analyze within 60 minutes of collection. Transport: Send room temp

Preferred Collection/Volume: LAV EDTA Volume: 4.0 mL Absolute Minimum Volume: 0.5 mL

TAT: 24 Hours Test Schedule: Daily Method: Turbidimetric Inhibition Immunoassay Hemoglobin A1c Test Facility: Airport Way Regional Laboratory SunnySide Medical Center (STAT Testing Only)

This is the Methodology.

Processing: Do not centrifuge Transport: Refrigerate if held.

Preferred Collection/Volume: Whole blood, fingerstick Volume: 0.1 mL wb Alternative Collection: LAV EDTA LAV Micro Absolute Minimum Volume : 0.5 mL

TAT: 1 Hour Test Schedule: Varies Method: Turbidmetric Inhibitor Immunoassay Hemoglobin A1C, Peds Test Facility: Beaverton Medical Office Interstate Medical Office Orchards Medical Office Skyline Medical Office

Pediatric A1Cs are performed for diabetic clinics at the Beaverton, Interstate, Ochards, and Skyline Medical Offices and are performed only during prearranged dates and times. This test is not available except under the stated conditions and locations. See Medical Office Supervisor for more information.

Preferred Collection: LAV EDTA Alternative Collection:

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LAV EDTA Microtainer Minimum Volume: 1 mL

TAT: 24 hours for routine specimens Testing Method: 5-Part diff Sysmex Hematology cell counter Stability:

{ Specimen is stable for 24 hrs at room temperature for all parameters.

{ Refrigerated specimens are stable for 72 hours for hemogram (ABC) parameters.

{ Specimens 48-72 hrs old require evaluation of all abnormal differentials. Hemoglobin and Hematocrit Rejection Criteria:

{ Clotted specimens

{ Hemolyzed specimens

{ IV contaminated specimens

Note: Test on site whenever possible if specimen is collected in a microtainer.

Processing: Handle microtainers ASAP and test on-site when possible. Transport: Transport specimens to Airport Way Laboratory.

Preferred Collection/Volume: LAV EDTA Volume : 4.0mL wb Absolute Minimum Volume: 0.5mL wb

TAT: 1 Week Test Schedule: Tuesdays Hemoglobin Evaluation Method: Test Facility: Airport Way Regional Laboratory

Processing: Do NOT centrifuge Transport: Transport to AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: Any

Hemoglobin Fetal Spec collected by nursing service or provider. Specimens that may be submitted Qualitative are Amniotic Fluid, Gastric Aspriate, Blood on diaper, ETC. See Kleihauer-Betke for Quant test.

Preferred Collection/Volume: GREEN NA HEP 6 Volume: 1.0 mL Plasma

TAT: Report available: Next Day Test Schedule: Monday-Friday Afternoon Hemoglobin Free Method: Colorimetric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Specimen Stability: Room Temperature: 24 hours

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Refrigerated: 7 days Frozen: 30 days Test Code: CHANTILLY TC 514

Processing: Centrifuge plasma within 1 hour of collection, aliquot the plasma into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: AP99 or UVAC First morning urine preferred but any random urine acceptable.

TAT: 2-3 days Test Schedule: M-F Hemosiderin Stain Method: Iron Stain Microscopic Qualitative Urine Test Facility: AWL Preparation: First morning urine preferred, random urine acceptable.

Processing: Smears must be prepared within 12 hours of collection. Refrigerate if delayed.

Preferred Collection/Volume: Blue CIT Volume: Full Tube Important: This test should only be collected 4 hours post last Enoxaparin dose.

TAT: 24 hours Test Schedule: M-F Method: Anti X-a Test Facility: AWL

Preparation: If using a butterfly, a citrate discard tube must be used first, when citrate is the first Heparin Assay tube collected. Quantitative Note, this test is for low molecular weight heparin. For unfractionated heparin, order referred test.

Heparin Assay LMW-Enoxaparin

Processing: Centrifuge immediately and aliquot 1.0 mL plasma into 2 aliquot tubes using a plastic transfer pipette. DO NOT USE FILTERS. Avoid buffy coat. Platelet poor plasma required. Freeze Transportation: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection/Volume: GRN Li Hep PST Vlolume: 1.0 mL p Alternative Collection: RED SST Volume: 1.0 mL s

TAT: 24 Hours Test Schedule: Daily Hepatic Function Panel Method: Colorimetric Test Facility: AWL, SMC, INT, LVK, SKY

Analytes include T bili, D bili, T protein, Alb, AST, ALT, and Alk phos

Processing: Centrifuge. DO NOT REMOVE CAP. Transport: Refrigerate if held.

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Preferred Collection/Volume: RED 10 Volume: 1.0 mL s Alternative Collection: RED SST Absolute Minimum Volume: 0.4 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Method: Luminescent Immunoassay Hepatitis A Antibody Test Facility: AWL

Screening for travel. This is Hepatitis A total (IgM and IgG combined). Order this test for an immunity screen. For diagnosis of acute hepatitis A infection, order HAS (IgM only). For OHSU patients, order a HAT

Processing: Centrifuge and Pour Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL s Alternative Collection: RED SST Absolute Minimum Volume: 0.4 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Hepatitis A Antibody Method: Luminescent Immunoassay IgM Test Facility: AWL

Order this test if Hep A IGM is requested.This test is for diagnosisof acute infection with Hepatitis A. For immunity screening (travel screen)order HAR (Hep A Total)

Processing: Centrifuge and Pour Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL s Alternative Collection: RED SST Absolute Minimum Volume: 0.2 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Hepatitis B Core Method: Luminescent Immunoassay Antibody Test Facility: AWL

If test is positive will reflex a Hep B Core AB IgM (HIG) FOR OHSU ORDER HBN

Processing: Centrifuge and Aliquot Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 0.5 mL s Alternative Collection: RED SST Absolute Minimum Volume: 0.3 mL s

Hepatitis B Core TAT: 24 Hours Antibody IgM Test Schedule: Mon - Fri Method: Luminescent Immunoassay Test Facility: AWL

This panel is reflexed from a positive Hep B Core Ab Total (HBC).

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Processing: Centrifuge and Aliquot Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL s Alternative Collection: RED SST Absolute Minimum Volume: 0.4 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Hepatitis B Surface Ab , Method: Luminescent Immunoassay Post Exposure Test Facility: AWL

Test for Employee exposed to needlesticks, etc or post vaccination Record Post exposure or Post vaccination

Processing: Centrifuge and Pour Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 2.0 mL s Alternative Collection: RED SST Absolute Minimum Volume: 0.4 mL s

TAT: 24 Hours Hepatitis B Surface Ab , Test Schedule: Mon - Fri Quant Method: Luminescent Immunoassay Test Facility: AWL

Processing: Centrifuge and pour Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL s Alternative Collection: RED SST Absolute Minimum Volume: 0.4 mL s

TAT: 24 Hours Hepatitis B Surface Test Schedule: Mon - Fri Antibody Method: Luminescent Immunoassay Test Facility: AWL

Processing: Centrifuge and Pour Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 2.0 mL s Alternative Collection: RED SST Absolute Minimum Volume: 1.0 mL s

Hepatitis B Surface TAT: 24 Hours Antigen Test Schedule: Mon - Fri Method: Luminescent Immunoassay Test Facility: AWL

Not a STAT test Add on stability: 3 days

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Processing: Centrifuge and aliquot Transport: Refrigerate if held.

Preferred Collection: 2 LAV EDTA Tubes Volume: 3.0mL (aliquoted plasma) Alternative Collection: 2 SST Tubes (aliquoted serum) Minimum Volume: 1.0mL

TAT: 1-4 days Testing Method: Real-Time PCR Testing Location: Airport Way Lab Molecular Department Specimen Stability Hepatitis B Virus DNA Room Temperature: 72 hours if centrifuged and aliquoted within 24 hours of collection. Refrigerated: 7 days if centrifuged and aliquoted within 24 hours of collection. Frozen: 42 days if centrifuged and aliquoted within 24 hours of collection. Specimen Rejection:

{ Specimens collected in a RED 10 , Li Hep, DRK Blue EDTA

{ Whole blood stored 2-25 c for >24hours

NOTE: Frozen Specimens may be frozen and thawed 5 times.

Processing: Centrifuge specimen within 24 hours of collection at 800 to 1600xg for 20 minutes, then aliquot into a referred test aliqout container. Note: Must rubber band both aliquot containers together. Transportation: Transport aliquoted specimens on ice to Airport Way Lab.

Preferred Collection/Volume: WHITE PPT Volume 1.0 mL Plasma Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: 8 days Test Schedule: Set up Monday and Thrusday Method: Polymerase Chain Reaction (PCR) • Sequencing Hepatitis B Virus Drug Test Facility: Quest Diagnostics Nichols Institute Resistance Genotype 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: This test is used to: 1. Identify HBV genotype (A-H) for epidemiology or prognostic purposes. 2. Detect hepatitis B virus (HBV) mutations associated with resistance to antiviral agents.

Specimen Stability: Room temperature: 72 hours Refrigerated: 7 days

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Frozen: 30 days Test Code: SJC TC 10529N

Processing: Centrifuge plasma within 2 hour of collection, aliquot the plasma into Referred Tests aliquot tube. Freeze Transport: Transport frozen on ice

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next day Test Schedule: Set up: Mon-Fri a.m Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Hepatitis Be Antibody Clinical Data: HBeAb appears in the early convalescence of HBV infection. With carrier state and chronic hepatitis, HbeAb may not develop.

Specimen Stability: Room temperature: 7 days Refrigerated: 14 days Frozen: Indefinitely Test Code: CHANTILLY TC 556

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Next Day Test Schedule: Monday-Friday Morning Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: HBeAg indicates active HBV replication. Infectivity is evaluated based on HBeAg Hepatitis Be Antigen and HBsAg. When HBeAg persists much longer than 10 weeks, the patient is likely to develop chronic hepatitis and be a carrier. See Hepatitis in the Infectious Disease chapter, Interpretive Information section.

Specimen Stability: Room Temperature: 7 Days Refrigerated: 14 Days Frozen: Indefinitely Rejection Criterion: Gross hemolysis or gross lipemia. Test Code: CHANTILLY TC 555

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: LAV EDTA Volume: 3.0 mL p Absolute Minimum Volume: 2.0 mL p

TAT: 24 Hours

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Test Schedule: Mon - Fri Method: Luminescent Immunoassay Test Facility: AWL

Fill LV6 or LV5 completely. If test is positive or equivocal, Hepatitis C RNA PCR Quantitative will be done

Hepatitis C Antibody Processing: Centrifuge and aliquot Transport: Refrigerate if held. AWL: Store frozen

Hep C AB R, Hepatitis C Antibody IgM + IgG (86803F EAP, Hepatitis C Antibody, HCR)

Preferred Collection/Volume: LAV EDTA Volume: 2.0 mL p

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning Method: Reverse Transcriptase-Polymerase Chain Reaction Line Probe Assay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Hepatitis C genotype is a predictor of response to interferon alfa-2b (non-type 1 Hepatitis C Genotyping are better responders) and to combination therapy with interferon and ribavirin (all types respond but dosage and duration of treatment is dependent on genotype; Type 1 requires extended treatment).

Specimen Stability: Room Temperature: 6 hours Refrigerated: 7 days Frozen: 90 days (-20c) Test Code: CHANTILLY TC 37811

Processing: Centrifuge within 1 hour of collection, aliquotthe plasma into Referred Tests aliquot tube. Freeze Transport: Transport frozen on ice

Preferred Collection/Volume: 6 mL LAV EDTA Volume: 3.0 mL p Absolute minimum volume is 3.0 mL plasma Alternative Collection: 2 LAV EDTA 5mL

TAT: 1-6 days Test Schedule: Tue, Thur Method: Real Time qPCR Test Facility: AWL: Molecular Diagnostics

Hepatitis C RNA PCR Clinical Data: n/a Quantitative Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Heparinized collection Specimen stored at 2-25 C > 6 hours.

Processing: Centrifuge specimen within 4 hours after collection. Aliquot sample into screw top vial. Freeze solid. Transport: Transport frozen on ice. Do not allow to thaw. Do not transport uncentrifuged or

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unfrozen.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Reports in 1 day Test Schedule: Sets up 2 days a week, A.M. Method: Qualitative Enzyme-Linked Immunosorbent Assay Test Facility: ARUP Laboratories 500 Chipeta Way Salt Lake City, UT 84108-1221

Hepatitis D Virus RNA Clinical Data: Hepatitis D agent occurs only in patients infected with HBV. The presence of Qual Hepatitis D Antibody and HBsAg indicate co-infection. Patients with Hepatitis D are more likely to develop fulminant hepatitis and chronic hepatitis than patients infected only with HBV. Hepatitis Delta Antigen detection is transient and occurs before development of Antibody.

Specimen Stability: Room temperature: Unacceptable Refrigerated: Unacceptable Frozen: 3 Months Test Code: ARUP TC 2006450

Processing: Centrifuge and aliquot into Referred Tests aliquot tube within one hour. Freeze Transport: Transport frozen on ice

Preferred Collection/Volume: Specimen must be fixed in 10% neutral buffered formalin. Fixation between 6-72 hours is recommended. Alternate Collection: 5 unstained positively charged slides cut 4-6 micron thick from formalin fixed, paraffin-embedded breast tissue and H&E slide

TAT: Report Available 4-6 days Test Schedule: Monday and Thursday Test Method: Fluorescence in situ Hybridization Testing Location: Airport Way Regional Loboratory Cytogenetics Department Her-2 FISH, Breast Only Specimen Stability: Room Temperature: Indefinite Refrigerated: Indefinite Frozen: Unacceptable

Processing: Pathology report must be available (Co-Path/HealthConnect). Report must include specimen identifiers, specimen site and type, type and duration of fixation, time to fixation (cold ischemic time) and pathologic diagnosis. Transportation: Room Temperature

Preferred Collection/Volume: Collection Instructions: Specimen must be fixed in 10% neutral buffered formalin. Fixation between 6 and 48 hours is recommended. Elasped time specimen was fixed in formalinshould appear on path report. This test is performed on formalin- fixed paraffin-embedded tissue from invasive or metastatic breast cancer. Other fixatives and noninvasive breast cancer specimens are not acceptable for testing. Formalin Fixed Paraffin Block Alternative Collection: 6 Unstained, positively charged slides Slide Holder (Formalin fixed); Room Temperature;

TAT: Report available: 4 Days Test Schedule: Monday-Sunday Morning Method: Fluorescence In Situ Hybridization

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Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Specimen Stability: Room Temperature: Indefinite Refrigerated: Indefinite Frozen: Unacceptable Fixatives other than formalin are not acceptable for testing. In breast cancer testing, a sample with only noninvasive carcinoma is not acceptable for testing. This test may be canceled and replaced by Test Code 416, Cytogenetics Communication, if no results can be given for the submitted specimen, for specimens that are 1+ and 2+ for Her-2/neu by . Nichols Chantilly accepts nonbreast carcinomas for HER2 FISH testing. Do not reject nonbreast carcinoma paraffin-block specimens for HER2 FISH testing. Other fixatives and noninvasive breast cancer specimens are not acceptable for testing. This test may be canceled and replaced by Test Code 416, Cytogenetics' Communication, if no results can be given for the submitted specimen. Formalin-fixed, paraffin-embedded tissue. Breast tissue biopsy, formalin-fixed, Her-2 Neu (Hercept) paraffin-embedded block. Pathology report or pathologic diagnosis must be included. Ship at room FISH temperature. Invasive or metastatic breast cancer formalin-fixed, paraffin-embedded tissue or charged/+slides from formalin-fixed, paraffin-embedded tissue. Specimen MUST be fixed in 10% neutral buffered formalin. Fixation between 6 and 48 hours is recommended. Pathology report must accompany paraffin block or slides. Information required in this report includes: Physician identification, specimen identifiers (case and block number), specimen site and type, tissue processing used (routine or microwave), type of fixative, time and duration of fixation, pathologic diagnosis, and IHC score, if performed elsewhere. Ship at room temperature. Do not freeze. Test Code: CHANTILLY TC 19859

Processing: Pathology report must accompany paraffin block or slides. Information required in this report includes: Physician identification, specimen identifiers (case and block number), specimen site and type, tissue processing used (routine or microwave), type of fixative, duration of fixation, pathologic diagnosis, and IHC score, if performed elsewhere. Ship at room temperature. Do not freeze. Nichols Chantilly accepts nonbreast carcinomas for HER2 FISH testing. Thus, we do not reject nonbreast carcinoma paraffin-block specimens for HER2 FISH testing. Transport: Room Temperature

Preferred Collection/Volume: RED SST Volume : 1.0 mL Serum Cause for Rejection: Grossly hemolyzed, grossly lipemic, or heat-inactivated serum samples are unacceptable

TAT: Report available: Next Day Test Schedule: Monday-Saturday Morning Method: Immunoassay Test Facility: AWL

Herpes Simplex 1 and 2 Clinical Data: Herpes Simplex Virus (HSV) is responsible for several clinically significant Ab human viral diseases, with severity ranging from inapparent to fatal. Clinical manifestations include genital tract infections, neonatal herpes, meningoencephalitis, keratoconjunctivitis, and gingivostomatitis. There are two HSV serotypes that are closely related antigenically. HSV type 2 is more commonly associated with genital tract and neonatal infections, while HSV type 1 is more commonly associated with infections of non-genital sites. Specific typing is not usually required for diagnosis or treatment. The mean time to seroconversion using the type specific assay is 25 days. The performance of this assay has not been established for use in a pediatric population, for neonatal screening

Processing: Centrifuge Transport: Trasport to AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning Method: Immunoflorescence Assay

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Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Herpes Simplex Virus (HSV) is responsible for several clinically significant human viral diseases, with severity ranging from inapparent to fatal. Clinical manifestations include genital tract infections, neonatal herpes, meningoencephalitis, keratoconjunctivitis, and gingivostomatitis. There are two HSV serotypes that are closely related antigenically. HSV type 2 is more commonly associated with genital tract and neonatal infections, while HSV type 1 is more commonly associated with infections of non-genital sites. IgM HSV antibodies in infants may be helpful in the diagnosis of neonatal infection. IgM antibody usually appears within the first 4 weeks Herpes Simplex of life in infected infants and persists for many months. IgM suggests a recent HSV exposure but Antibody IgM does not differentiate between primary infection and reactivation.

Specimen Stability: Room Temperature: 7 Days Refrigerated: 14 days Frozen 30 Days TC# 90849

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transportation: Tranport to AWL in blue tote

{ Swab submitted in a Viral Transport Media or equivalent (UTM)

{ 1 ml fluid (amniotic, pericardial, pleural, sputum, vitreous, urine or bronchial brush/washing/lavage) submitted in a sterile leak-proof container

{ 1 ml whole blood or plasma collected in EDTA tube or ACD-B tube

{ 1 ml serum

Minimum Volume: 0.3 mL NOTE: CSF specimens should be ordered as HSV 1&2 CSF PCR (performed at AWL)

TAT: Report is available in 1 day from receipt of specimen at the testing location. Herpes Simplex Virus Testing Location: by PCR Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Test Code: 14426

Specimen Stability:

Room temperature: 48 hours Refrigerated: 7 days Frozen: 30 days (do not freeze whole blood)

Rejection Criteria: Specimens collected in heparin tubes. Frozen whole blood.

.

Processing: Refrigerate Specimen Transportation: Send specimen on ice to Airport Way Lab Referred Test Department.

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AWL: Freeze upon receipt (except whole blood)

Preferred Collection: Urine Volume: 4 mL Urine Minimum Volume : 0.95 mL

TAT: Report available in 3-9 days Testing Location: ARUP Laboratories 500 Chipeta Way Hippuric Acid, Urine Salt Lake City, UT 84108-1221 TC# 0091175 www.aruplab.com

Transport: Refrigerated in a referred test aliquot container Specimen Stability: Refrigerated: 14 day(s) Frozen: 1 month(s)

Preferred Collection/Volume: 1 LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: 3 Days Test Schedule: Tuesday, Friday Morning Method: Immunoassay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Histamine is a mediator of the allergic response. Histamine release causes itching, flushing, hives, vomiting, syncope, and even shock. In addition, some patients with gastric carcinoids may exhibit high concentrations of Histamine.

Histamine Patient Preparation: For Clinician Instruction Only: Avoid taking allergy causing drugs, antihistamines, oral corticosteroids, and substances which block H2 receptors for at least 24 hours before collection.

Specimen Stability: Room Temperature: 24 Hours Refrigerated: 24 Hours Frozen: 7 Days Rejection Criterion: Samples that have hemolysis or lipemia are unacceptable. Test Code: CHANTILLY TC 36586 TO SJC TC 24166P

Processing: Centrifuge immediately aliquot into Referred Tests aliquot tube. Freeze Rejection Criterion: Samples that have hemolysis or lipemia are unacceptable. Transport: Transport frozen on ice

Preferred Collection/Volume: Add 10.0 mL 6N HCl to jug prior to collection. Patient should refrigerate jug during collection. 24-hour collection container (10.0 mL 6N HCL) Volume 4.0 mL Urine

TAT: Report available: 1 day

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Test Schedule: Set up: 2 days a week a.m Method: Enzyme Immunoassay (EIA) Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Histamine is a mediator of the allergic response. Histamine release causes itching, flushing, hives, vomiting, syncope, and even shock. In addition, some patients with gastric carcinoids may exhibit high concentrations of Histamine. Patient History: Avoid direct sunlight. Avoid taking allergy causing drugs, antihistamines, oral corticosteroids and substances which block H2 receptors 24 hours prior to collection.

Histamine 24 Hour Patient Preparation: For Clinician Instruction Only Patient should refrain from taking allergy Urine causing drugs, antihistamines, oral corticosteroids, and substances which block H2 receptors for at least 24 hours prior to specimen collection.

Specimen Stabiltiy: Room Temperature 48 Hours Refrigerated: 14 Days Frozen: 14 Days Test Code: SJC TC 4946X

Processing: Mix sample well and Aliquot 4.0 mL urine into sterile leak proof urine cup. Note on sample total volume and preservative used during collection. Transport: Refrigerate if held.

Preferred Collection/Volume: RED SST Volume: 1mL Serum. Alternative Collection: RED 10 Volume: 1mL Serum

TAT: Next Day Test Schedule: Mon., Wed., Fri. a.m Method: Enzyme Immunoassay (EIA) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Histone Antibodies, Clinical Data: Histone antibody is present in 80-95% of patients with drug-induced systemic Qualitative lupus erythematosus (SLE), 20-50% pf patients with idiopathic SLE, and infrequently in patients with other autoimmune connective tissue diseases.

Patient Preparation: Overnight fasting preferred

Specimen Stability: Room temperature: 8 hours Refrigerated: 7 days Frozen: 30 days Test Code: Quest TC 9012

Processing: Centrifuge and aliquot serum into a referred tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: 2-3 Days Test Schedule: Histoplasma Antibody, Complement Fixation, Serum Monday-Friday Morning Histoplasma Antibody, Immunodiffusion, Serum Tuesday, Thursday, Saturday Morning Method: Complement Fixation Immunodiffusion

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Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Histoplasma Antibody, Complement Fixation, Serum: Histoplasmosis, caused by infection by H. capsulatum, is usually asymptomatic or mild. Some patients develop chronic pulmonary histoplasmosis or acute disseminated histoplasmosis that is often life-threatening. Complement fixation assesses antibodies to the yeast and mycelial forms. Approximately 90-95% of patients with histoplasmosis have detectable antibodies. Histoplasma Antibody, Immunodiffusion, Serum:Histoplasmosis, caused by infection by H. capsulatum, is usually asymptomatic or mild. Some patients develop chronic pulmonary histoplasmosis or acute disseminated histoplasmosis that is often life-threatening. Immunodiffusion Histoplasma Antibody assesses the presence of proteins antigens M and H that often occur independently. Approximately 70% of patients with histoplasmosis have detectable antibodies by immunodiffusion

Specimen Stablitiy: Room Temperature: 7 Days Refrigerated: 14 Days Frozen 30 Days Test Code: CHATNILLY TC 74652

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: Random urine submitted in sterile leakproof container Volume: 10mL u Absolute Minimum Volume: 1mL u

TAT: 4 days Test Schedule: Monday-Friday am Test Method: Immunoassay Testing Location: Focus Diagnostics, Inc via Quest Chantilly 5785 Corporate Ave, Suite 200

Cypress, CA 90630 Histoplasma Antigen Clinical Significance Histoplasma galactomannan is frequently detected in urine from patients with disseminated histoplasmosis .

Specimen Stability: Room Temperature: 7 days Refrigerated: 14 days Frozen: 30 days TC# 91212

Processing: Transport : To AWL in tote

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.-4 mL Serum

Test Schedule: M-W-F, 8am Method: Heparin-dependent platelet activation (ATP release) detected by luminescence. Test Facility: OHSU Laboratories Homeostasis and Thrombosis 3181 S.W. Sam Jackson Park Road

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Portland, OR 97239

Patient History: Note patient heparin therapy

Specimen Stability: HIT-PF4 CRITICAL FROZEN Test Code: OHSU TC 068-8000

Processing: Centrifuge and aliquot into x 2 Referred Tests aliquot tube. Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: 1 LAV EDTA (Whole Blood) Volume: 1.0 mL Whole Blood

TAT: Report available: Next Day Test Schedule: Monday, Wednesday, Friday Morning Method: Polymerase Chain Reaction Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: HIV-1 DNA Real-Time PCR is a sensitive method for detection of proviral DNA. It HIV 1 DNA PCR has been used in the management of perinatal HIV infection. HIV-1 RNA has recently been Qualitative confirmed equally as sensitive and specific for this purpose.

Specimen Stability: Room Temperature: 6 Days Refrigerated: 6 Days Frozen: Unacceptable Rejection Criterion: gross hemolysis or gross lipemia Test Code: CHANTILLY TC 3525

Processing: Do not centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 4 days Test Schedule: Monday, Thursday Morning Method: ELISA (Enzyme Linked Immunosorbent Assay) Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave. Cypress, CA 90630

Clinical Data: The measurement of HIV p24 (core) antigen is performed by the Immune Complex Dissociation (ICD) modification of the standard p24 antigen assay. This modification HIV 1 P24 Antigen results in a significant increase in the sensitivity of the p24 antigen assay. All specimens found positive are confirmed by neutralization for an additional charge. Quantitation of p24 antigen has been shown to be useful for detection of circulating, replicating, as well as nonviable HIV. The measurement of p24 antigenemia using the ICD modification may be of significant value for the detection of early infection and as a marker of disease progression and therapeutic response. Furthermore the p24/ICD assay has shown significant value for the early detection of HIV infection in neonates and may supplement other diagnostic tests for neonatal infections, such as HIV-1 PCR. Reactive specimens are confirmed by a neutralization method for an additional charge.

Specimen Stability: Room Temperature: Unacceptable Refrigerated: Unacceptable Frozen: 2 years Failure to submit a frozen sample will result in cancellation. Test Code: CHANTILLY TC 36444 TO FOCUS TC 41080

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Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze. Transport: Transport frozen on ice

Preferred Collection/Volume: LAV EDTA 6mL Volume: 3.0 mL p. Alternative Collection: 2 LAV EDTA 5mL

TAT: 1-6 days Test Schedule: Tuesday and Thursday Method: Real Time qPCR. Test Facility: Airport Way Regional Laboratory, Molecular Diagnostics

Clinical Data: HIV consent form is not required for this test. HIV 1 RNA PCR Patient History: n/a Quantitative Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Heparinized plasma serum samples and specimen stored at 2-25 C > 6 hours.

Processing: Centrifuge specimen within 4 hours of collection. Aliquot 3.0 mL plasma from cells into plastic screw-top aliquot tube. Transport: Transport to Airport Way Regional Laboratory on ice.

Preferred Colllection/Volume: SSRED Collection Container Must Be Submitted-Do Not Aliquot Volume: 2.0mL s Absolute Minimum Volume: 0.5mL s

TAT: 24 hours Test Schedule: Monday through Friday Test Method: Chemiluminescent Immunoassay HIV 1/2 Antibody HIV Testing Location: Airport Way Regional Laboratory 1 Ag HIV 1/2 Ab Confirmation reflex testing of all reactive results performed at Airport Way Regional Lab.

Specimen Processing: Centrifuge-Do Not Aliquot Transportation: Transport to Airport Way Regional Laboratory

HIV 1/2 Antibody/HIV 1 Antigen (87381A EAP, HIV 1/2 Antibody)

Preferred Collection/Volume: RED SST Collection Container Must Be Submitted-Do Not Aliquot Volume: 2.0 mL s Absolute Minimum Volume: 0.5mL s

HIV 1/2 Antibody, TAT: 24 Hours Prenatal Test Schedule: Mon - Fri Method: Luminescent Immunoassay Test Facility: AWL

Western Blot reflex testing of all reactive results performed at Quest Diagnostics, Chantilly, Va.

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Includes Western Blot confirmation of all positive results

Processing: Centrifuge. DO NOT aliquot. Transportation: Transport to AWL in blue tote. Refrigerate if held.

HIV 1/2 Antibody, Prenatal ( 87389A EAP, HIV 1/2 Antibody, Prenatal)

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next day Test Schedule: Set up: Tues, Thurs Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: HIV-2 is closely related to HIV-1 regarding nucleic acid sequence and clinical disease. HIV-2 is endemic to West Africa with nearly all cases in the United States identified in citizens or travelers from West Africa. Rarely, HIV-1 Western blot indeterminate results may be HIV 2 Ab Confirm due to HIV-2 infection in a patient who has been exposed to HIV-2. The Immunoblot is useful to confirm repeatedly reactive EIA results.

Patient Preparation: Panel automatically reflexed by Quest for pos/borderline HIV-1/2 scree (HVA) and neg/borderline HIV-1 Western Blot (HWB).

Specimen Stability: Room temperature: 7 days Refrigerated: 14 days Frozen: 30 days Test Code: CHANTILLY TC 37363

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: 2 Days Test Schedule: Tuesday, Friday Morning Method: Immunoblot Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: HIV-2 is closely related to HIV-1 regarding nucleic acid sequence and clinical HIV 2 Ab Immunoblot disease. HIV-2 is endemic to West Africa, with nearly all cases in the United States identified in citizens or travelers from West Africa. Rarely, HIV-1 Western blot indeterminate results may be due to HIV-2 infection in a patient who has been exposed to HIV-2. The Western blot is useful to confirm repeatedly reactive EIA results.

Patient Preparation: HW2 automatically reflexed by Quest when HIV-2 EIA (HVE) is repeadtedl reactive.

Specimen Stability: Room Temperature: 7 Days Refrigerated: 14 Days Frozen: 30 Days Test Code: CHANTILLY TC 34313

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Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Report available: 2 days Test Schedule: Set up: Monday-Friday Morning Method: Wester Blot (WB) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: HIV-2 is closely related to HIV-1 regarding nucleic acid sequence and clinical disease. HIV-2 is endemic to West Africa with nearly all cases in the United States identified in citizens or travelers from West Africa. Rarely, HIV-1 Western Blot indeterminate results may be HIV Antibody by due to HIV-2 infection in a patient who has been exposed to HIV-2. The Western Blot is useful to Western Blot confirm repeatedly reactive EIA results.

Patient Preparation: HWB is automatically reflexed when the screening test (HVA) is positive. HWB, if negative will reflex HIV-2 EIA with reflex to Immunoblot (HVE) at Quest.

Specimen Stability: Room temperature: 7 days Refrigerated: 14 days Frozen: 30 days Test Code: CHANTILLY TC 37708

Processing: Tube must be labeled with patient identifier and submitted only for HIV testing. HIV-1 Western Blot requires original tube for testing. Do not aliquot. Transport: Refrigerate if held.

Preferred Collection/Volume: X 2 LAV EDTA Volume: 4.0 mL Plasma

TAT: Report available: 7 Days Test Schedule: Monday-Friday Morning Method: RT-PCR and DNA Sequencing Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Please note: This test can only be performed reliably on specimens with a viral HIV Genotype load of at least 2000 copies/mL.

Specimen Stability: Room Temperature: Unacceptable Refrigerated: 6 days Frozen: 3 months (-20c) Test Code: CHANTILLY TC 11509

Processing: Centrifuge plasma within 6 hour of collection, aliquot the plasma into Referred Tests aliquot tube. Freeze Transport: Transport frozen on ice

Preferred Collection/Volume: Collect Mon-Friday Only Specimen must reach AWL on Fridays by noon x 4 LAV EDTA Volume: 28 mLs

Test Facility: American Red Cross

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3131 North Vancouver Avenue Portland, OR 97227

Keep specimen at room temp. MUST REACH ARC WITHIN 24 HOURS. Complete ARC tissue HLA A, B and C typing form. Record CBC and ABO/RH results on form. Antigen Type American Red Cross-HLA

Processing: Do not centrifuge. Specimen must reach AWL on Fridays by noon. Transport: Send Room Temperature

Preferred Collection/Volume: Collect Mon-Thursday Only NO Gel Barrier RED 10 Volume: 5.0 mL serum NO Gel Barrier

Test Facility: American Red Cross 3131 North Vancouver Avenue HLA Ab Screen Portland, OR 97227

Keep specimen at room temp. MUST REACH ARC WITHIN 24HOURS. Complete ARC tissue typing form. Record CBC and ABO/RH results on form. American Red Cross-HLA

Processing: Centrifuge and aliquot into a Referred Tests aliquot tube. Transport: Send Room Temperature

Preferred Collection: LAV EDTA Minimum Volume: 1mL Note: Collect specimens Monday-Friday only. Specimens must reach the Referred Test department by 1400 on Fridays.

TAT: Report available next day from the day specimen was received at the testing location. Testing Schedule: Monday.-Saturday a.m., p.m. Methodology: Flow Cytometry Testing Location: HLA B27 Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 TC# 528 Specimen Stability: Room Temperature: 72 hours Refrigerated: 72 hours Frozen: Unacceptable Rejection Criteria: Light hemolysis, moderate hemolysis, serum, frozen specimens, lithium heparin tubes, EDTA (royal blue-top) tubes.

Clinical Data: HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's disease. Ankylosing spondylitis affects 1 in 1000 caucasians. Ankylosing spondylitis is 10 times more common among individuals with HLA-B27

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compared to individuals without this antigen.

Processing: Do not Centrifuge. Note: Refrigerate specimen, if held. Transport: Place LAV EDTA tube in a thermos marked room temperature, send to Airport Way Laboratory in blue tote. Note: Core Lab, take specimen to the Referred Test department immediately after the specimen has been received.

Preferred Collection/Volume: A separate Yellow ACD or LAV EDTA (lavender-top) tube must be submitted for all HLA testing. If submitted with any other test requiring an Yellow ACD or LAV EDTA tube, please submit a separate tube for this test. Do not open tube. Yellow Top ACD-A (Whole Blood) Volume 10.0 mL Whole Blood Alternative Collection: Yellow ACD B or X 2 LAV EDTA Volume: 10.0 mL Whole Blood.

TAT: Report available: 8 Days Test Schedule: Monday-Friday, a.m Method: Polymerase Chain Reaction Amplification followed by Sequence Specific Oligonucleotide Probes (PCR-SSO) HLA Celiac Disease Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Specimen Stability: Room temperature: 7 days Refrigerated: 7 days Frozen: Unacceptable Test Code: CHANTILLY TC 17135

Processing: Do Not Centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume: Collect Mon-Friday Only Specimens collected on Friday must reach AWL by noon. Record in order notes if patient is donor or recipient. x 4 LAV EDTA Volume: 24 mLs

Test Facility: American Red Cross 3131 North Vancouver Avenue Portland, OR 97227

HLA DR Typing Clinical Data: I ncludes donor and recipient typing for DR antigens.

Keep specimen at room temp. MUST REACH ARC WITHIN 24HOURS. Complete ARC tissue typing form. Record CBC and ABO/RH results on form. Check order notes and record on form if patient is donor or recipient. American Red Cross-HLA

Processing: Do not centrifuge. Transport: Send Room Temperature

Preferred Collection/Volume: (x 4) 6 mL LAV EDTA Alternate Collection: Yellow ACD Volume: 24mL wb Absolute Minimum: 14mL wb ***Please Note***A CBC must be ordered with this test. Collect this test

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Monday through Thursday only***

Test Facility: American Red Cross 3131 North Vancouver Avenue Portland, OR 97227

MUST REACH ARC WITHIN 24HOURS HLA DR2 DQW1 Keep specimen at room temp Complete American Red Cross HLA Laboratory Requisition Record CBC and ABO/RH results on form

Processing: Do not centrifuge. Transport: Transport to AWL in blue tote.

Preferred Collection/Volume: Collect Mon-Thursday Only x 4 LAV EDTA and x 1 RED 10 Volume: LAV EDTA 24 mLs Serum 3mLs NO Gel Barrier Tubes

Test Facility: American Red Cross 3131 North Vancouver Avenue Portland, OR 97227 HLA Panel (ABC Type, Clinical Data: Includes HLA AB Typing, HLA AB Screen and Platelet AB testing. Complete ARC Class Screen, Plt Ab) Tisue Typing form (from Client Services). Must include a CBC, ABO/RH order.

Keep specimen at room temp. MUST REACH ARC WITHIN 24HOURS. Complete ARC tissue typing form. Record CBC and ABO/RH results on form. Test Code: American Red Cross-HLA

Processing: Do not centrifuge LAV EDTA RED 10: Centrifuge and aliquot into a Referred Tests aliquot tube. Transport: Send Room Temperature

Preferred Collection/Volume: Yellow A or Yellow B Volume: 10mL wb Alternative Collection: LAV EDTA Volume: 3mL wb

TAT: 8 Days Test Schedule: Monday-Friday am Test Method: Polymerase Chain Reaction (PCR) • Sequence Specific Oligonucleotide Probes (SSO) Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 HLA-B 5701 Typing Clinical Significance HLA-B*5701 has been shown in retrospective clinical studies to be associated with Abacavir hypersensitivity reaction (HSR) in HIV patients. In Abacavir-naive patients, HLA-B*5701 genotyping may be useful for risk stratification. Limitations A negative result does not rule out the risk for ABC HSR and clinical vigilance should be maintained regardless of the result. The frequency of HLJ/-B5701 is approximately 8% in Caucasians and 2% in African Americans.

Specimen Stability Room Temperature: 7 days

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Refrigerated: 7 days Frozen: unacceptable Cause for rejection: Specimens collected in heparinized tubes (Gn, green-top tube, sodium or lithium heparin) are unacceptable. Frozen specimens are unacceptable. TC# 19774

Processing: Do Not Centrifuge Transportation: Tranpsport to AWL in blue tote

Preferred Collection/Volume: GRN Li Hep PST Volume: 2.0 mL s Alternative Collection: LAV EDTA Absolute Minimum Volume: 1.0 mL

TAT: 1 week Test Schedule: Wednesday Evenings Homocysteine Total Method: Chemiluminescent Immunometric Assay Test Facility: Airport Way Regional Laboratory

N/A

Processing: Centrifuge as soon as possible. Refrigerate if held. Transport: To AWL in blue tote

Preferred Collection/Volume: Thin Prep PreservCyt Media/2.0 mL Co-collect HPV sample for women 30 and over using the pink handled broom and ThinPrep liquid cytology media Alternative Collection: 1.0 mL PerservCyt Media

TAT: 1-4 days Test Schedule: Run daily, Mon - Fri Method: Invader Assay HPV Cotest, High Risk, Test Facility: AWL: Molecular Diagnostics DNA, Fluorescence Resonance Energy Clinical Data: n/a Transfer Patient History: n/a Patient Preparation: n/a Labeling: n/a

Rejection criteria: < 1 mL specimen received.

Processing: Agitate the broom in the media, then discard the intact broom and handle. Transport: Room Temperature.

Preferred Collection/Volume: Digene PHV II Sampler or Cell pellet of SurePath pap specimen

TAT: Report available: 2 days Test Schedule: Monday-Friday Morning Method: Hybrid Capture Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: HPV is the causative agent of cervical dysplasia and cervical carcinoma. This assay only detects high-risk HPV types, 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68.

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Specimen Stability: Cervical broom or Digene ® cervical sampler (brush) __Room temperature: 14 days __Refrigerated: 21 day __Frozen: 90 days Cervical biopsy __Room temperature: 24 hours __Refrigerated: 24 hours __Frozen: Indefinitely Cytyc ® PreservCyt ® (ThinPrep ®) __Room temperature: 90 days __Refrigerated: 90 days HPV DNA HR Sendout __Frozen: Unacceptable Cytorich ® fraction, SurePath ®: __Room temperature: 30 days __Refrigerated: 30 days __Frozen: Unacceptable Test Code: CHANTILLY TC 10161

Processing: Digene Sampler Deliver directly to Referred Test . SurePath resuspend cell pellet in 2 mL Cytolyt; Deliver direclty to Referred Test; tracking Make an order note indicating if Digene or SurePath HPV Transport: Refrigerate if held.

Preferred Collection/Volume: Thin Prep PreservCyt Media/ 2.0 mL Collect the HPV sample using the pink handled broom and ThinPrep liquid cytology media. Agitate the broom in the media, then discard the intact broom and handle. Alternative Collection: 1.0 mL PerservCyt Media

TAT: 1-4 days Test Schedule: Run daily, Mon - Fri Method: Invader Assay Test Facility: AWL: Molecular Diagnostics HPV High Risk DNA, Fluorescence Resonance Clinical Data: Order this panel only if no PAP specimen is being collected on the patient at this time. This is an HPV order without a companion PAP specimen. Energy Transfer Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection criteria: < 1 mL specimen received.

Processing: Agitate the broom in the media, then discard the intact broom and handle. Transport: Room Temperature.

Preferred Collection/Volume: Cerebral Spinal Fluid submitted in a sterile container Volume: .5mL csf Specimen Handling Processing: Tranpsort to AWL on ice HSV 1& 2 CSF PCR AWL: Freeze upon receipt

TAT: 1-3 days Schedule: Monday-Friday once per day Test Method: Real-Time Polymerase Chain Reaction (PCR) Tested At: Airport Way Laboratory

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Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: Next Day Test Schedule: Monday.-Friday., a.m Method: Chemiluminescence Assay Immunoassay LIA Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 HTLV 1 and 2 Ab, Reflex To Western Blot Clinical Data: HTLV-I is associated with adult T-cell lymphoblastic leukemia and B-cell chronic lymphocytic leukemia. HTLV-II is less common and is associated with neoplasias of the CD8 T lymphocytes. Blood donor screening began in 1998.

Specimen Stability: Room temperature: 8 hours Refrigerated: 7 days Frozen: 30 days Test Code: CHANTILLY TC 36175

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Note if serum or plasma on specimen. Transport: Transport on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: 2 Days Test Schedule: Tues.-Fri., a.m Method: Line Immunoassay (LIA) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: HTLV-I is associated with adult T-cell lymphoblastic leukemia and B-cell chronic HTLV 1 and 2 Western lymphocytic leukemia. HTLV-II is less common and is associated with neoplasias of the CD8 T Blot lymphocytes. Blood donor screening began in 1998. Line Immunoassay (LIA) is used for confirmation of Antibody testing. Do not order. HTW automatically reflexes from HTL (Screen) Quest Chantilly, TC# 36175.

Specimen Stability: Room Temperature: 8 hours Refrigerated: 7 days Frozen: 30 days This test is for confirmation only. For HTLV-I/II screening, please use Test Code 36175. Test Code: CHANTILLY TC 8511

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Note if serum or plasma on specimen. Transport: Transport on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: 4 Days Test Schedule: Tuesday, Thursday, Sunday Night

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Method: Indirect Immunofluorescence Assay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Paraneoplastic syndrome involves nonmetastatic systemic effects that accompany malignant disease. Antineuronal Nuclear Antibody (Anti-Hu) is found in 5-10% of patients with small cell carcinoma of the lung. Anti-Hu is associated with subacute syndrome of encephalomyeloradiculopathy, sensory neuropathy, and autoimmune neuropathy, predominantly affecting the gastrointestinal tract. <> If the Hu Ab, IFA, Serum, result is positive, then Hu Ab, Western Blot, Serum, will be performed at an additional charge (CPT: 84181). If the Hu Ab, Western Blot, Serum, result is positive, then Hu Ab Titer, Serum, will be performed at an additional Hu Ab IFA charge (CPT: 86256).

Patient Preparation: A fasting sample is required.

Specimen Stability: Room Temperature: 7 Days Refrigerated: 14 Days Frozen: 21 Days Test Code: CHANTILLY TC 37053 TO SJC 37053

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: Yellow ACD A or B Volume: 6.0 mL wb Alternative Collection: LAV EDTA

TAT: 2 weeks Test Schedule: Weekly Test Facility: OHSU Laboratories Molecular Diagnostic Center 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Clinical Data: Testing is diagnostic for normal/presymptomatic/disease states. Huntington Huntington Disease disease is an autosomal dominant disease with onset in adult life in most cases. Extensive Mutation Analysis experience with affected individuals and their families has indicated that it is mandatory for individuals requesting presymptomatic testing to participate in an approved multidisciplinary Huntington disease testing protocol Patient History: An Ordering Physicians Statement must accompany all requests for testing. This can be found at http://www.ohsu.edu/pathology/wardman/forms/huntington.pdf

Specimen Stability: Room Temp Test Code: OHSU TC 102-7060

Processing: Do not centrifuge Transport: Send Room temperature.

Preferred Collection/Volume: RED 10 Volume : 5.0mL s Minimum Volume: 2.5mL s

Test Schedule: Performed once per week. Test Facility: OHSU Laboratories Occupational and Environmental Allergy Lab 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Hypersensitivity Clinical Data: Precipitin panels demonstrate the presence of precipitating antibody Pneumonitis Precipitins (predominantly IgG and IgM) by immunodiffusion (Ouchterlony) in gel medium. This panel contains

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Panel the most common antigens involved in hypersensitivity pneumonitis including several species of thermophiles, Aspergillus, Penicillium, Alternaria, and others. Most clinically significant results occur when multiple and sharp precipitin lines are found. A trace precipitin band is rarely important and a weak band is equivocal.

Specimen Stability: Refrigerated Test Code: OHSU TC 064-0908

Processing: Centrifuge and aliquot. Transport: Transport in blue tote to AWL. Refrigerate if held.

Preferred Collection/Volume: RED SST Volume: 0.5mL s Alternative Collection: RED 10 Absolute Minimum Volume: 0.2mL s

TAT: 24 Hours IgA Test Schedule: Daily Method: Turbidometry Test Facility: AWL

Processing: Cent. aliquot if RED 10. Refrigerate if held

Preferred Collection/Volume: RED 10 Volume: 2.0mL s Alternative Collection: RED SST Absolute Minimum Volume: 0.5mL s

TAT: 1 Week IgE Test Schedule: Tue Method: Chemiluminescent Immunometric Assay Test Facility: AWL

Processing: Cent, Aliquot, and Refrigerate. Freeze after 72 Hrs

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next day Test Schedule: Set up: Mon-Fri evening shift Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 IGF Binding Protein-3 Specimen Stability: Room temperature: 7 days Refrigerated: 14 days Frozen: 28 days (glass tubes unacceptable) This test is for confirmation only. For HTLV-I/II screening, please use Test Code 36175. Test Code: CHANTILLY TC 34458

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED SST Volume: 0.5mL s Alternative Collection: RED 10

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Absolute Minimum Volume: 0.2mL s

TAT: 24 Hours Test Schedule: Daily IgG Method: Turbidometry Test Facility: AWL

Processing: Cent. aliquot if Red Top Tube. Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Saturday Afternoon Method: Nephelometric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 IgG Subclass 4 Patient Preparation: Overnight fasting is preferred

Specimen Stability: Room Temperature: 72 hours Refrigerated: 21 days Frozen: 6 months Test Code: CHANTILLY TC 143422

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 3.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Saturday Afternoon Method: Nephelometric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Measurement of IgG subclasses may be helpful in the management and understanding of immunodeficiency diseases, hypersensitivity states, and conditions involving IgG Subclasses susceptibility to infection. See IgG Subclasses in the Immunology chapter, Interpretive Information section.

Patient Preparation: Overnight fasting is preferred

Specimen Stability: Room Temperature: 72 hours Refrigerated: 21 days Frozen: 6 months Test Code: CHANTILLY TC 7903X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED SST Volume: 0.5mL s Alternative Collection: RED 10

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Absolute Minimum Volume: 0.2mL s

TAT: 24 Hours Test Schedule: Daily IgM Method: Turbidometry Test Facility: AWL

Processing: Cent. Aliquot if RD10. Refrigerate

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 3.0 mL Serum

TAT: Report available: 2 Days Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Imipramine is a tricyclic antidepressant used in the treatment of depressive disorders. The drugs are specifically useful in treatment of autonomous or endogenous Imipramine Level depressions. Imipramine is metabolized to desipramine, which is also an active tricyclic antidepressant. Toxicity can lead to arrhythmias, coma, and death.

Patient Preparation: Collect just before next dose or at least 12 hours after last dose.

Specimen Stability: Room Temperature: 5 days Refrigerated: 7 days Frozen: 1 month Test Code: CHANTILLY TC 892

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL s Absolute Minimum Volume: 0.5 mL s

TAT: 24 Hours Test Schedule: Mon-Fri Immunofixation Method: Electrophoresis Test Facility: AWL

For identification of monoclonal protein. Serum protein electrophoresis (SPE) typically ordered first

Processing: Cent, aliquot, refrigerate if held

Preferred Collection/Volume: Urine AP99 Volume: 50.0 mL u Absolute Minimum Volume: 5.0 mL u

TAT: 24 Hours Immunofixation Urine Test Schedule: Mon-Fri Method: Electrophoresis Test Facility: AWL

For identification of monoclonal protein. Urine protein electrophoresis. is typically ordered first (see panel UPE) and if indicated urine immunofixation will be performed

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Processing: Refrigerate if held

Preferred Collection/Volume: RED 10 Volume: 2.0mL s Alternative Collection: RED SST Absolute Minimum Volume: 1.0mL s

TAT: 24 hours Test Schedule: Daily Immunoglobulins Method: Immunoassay Quantitative Test Facility: AWL

This panel includes an IGA,IGM,and IGG. Each may be ordered individually for following patient results

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection: Nasopharyngeal swabs (NP), Naspharyngeal washings, or Nasopharyngeal aspirates placed immediately into Viral Transport Media (UVT). Alternative Collection: Nasopharyngeal washings and Nasopharyngeal aspirates placed into sterile container.

TAT: Results reported within 3 hours of receipt at testing location. Please allow for transport time for specimens collected at non testing locations. Positive results will be called to the provider. Test Schedule: Sunday- Saturday Method: Real Time PCR Test Location: Sunnyside and Westside Medical Centers

Clinical Data: Influenza A/B PCR testing will only be available for Emergency Room patients, inpatients, and for outpatients with risk factors (pregnancy, children 5 years old and younger, and immunosuppressed patients). This testing will be performed 24/7 at both Medical Center Laboratories. Influenza A/B/H1N1 PCR Note: Respiratory Virus Panel is available for viruses other than influenza if suspected. See Respiratory Virus Panel in the Clinician Laboratory Guide.

Rejection Criteria: <300 uL specimen Nasopharyngeal swab not submitted in UVT media Specimen transported ambient Bronchial Lavage (BAL), Endotrachial aspirate, sputum or excessively mucoid specimens (i.e; non-pipettable) Note: Respiratory Virus Panel is available for viruses other than influenza if suspected. See Respiratory Virus Panel in the Clinician Laboratory Guide

Transport: Transport immediately to testing location. Note: Specimens collected at Sunnside and Westside campus locations may be transported to laboratory without refrigeration. All other locations must send specimens on ice.

Preferred Collection/Volume: RED 10 Volume: 4.0 mL s Absolute Minimum Volume: 2.0 mL s

TAT: 24 Hours

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Test Schedule: Mon-Fri Method: Test Facility: AWL

Test includes: English Plantain, Lambs Quarter, Rough Pigweed, June Grass, Meadow Fescue, Inhalant IgE Panel Perennial Rye, Hazel Nut Tree, Birch, Alder, Elm, Willow, Cottonwood, Oak, Maple/Boxelder, Walnut Tree, White Ash, D.Farinae, Comprehensive D.Pteronyssinus, Dog Dander, Cat Dander, Goose Down, Duck Feathers, Aspergillus Fumigatus, Helminthosporium Halodes, Cladosporium Herbarum, Alternaria, Sheep Sorrel, Mugwort, Scotch Broom

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RED 10 Volume: 4.0 mL s Absolute Minimum Volume: 2.0 mL s

TAT: 24 Hours Test Schedule: Mon-Fri Inhalant IgE Panel Method: Immunoassay Limited Test Facility: AWL Test includes the following allergens: D.Pteronyssinus (Mites), Derm.Farinae (Mites), Cat Dander, Dog Dander, Alternaria Alternata, Cladosporium Herbarum, Timothy Grass, Sheep Sorrel, English Plantain, Hazel Nut Tree, Birch, Cottonwood, Maple/Boxelder, Oak

Processing: Centrifuge, aliquot, refrigerate if held

Preferred Collection/Volume: RED 10 NO Gel Barrier Tubes Volume: 2.0 mL s Absolute Minimum Volume: 1.0 mL s

TAT: 48 Hours Inhibin A Test Schedule: Mon, Wed, Fri Method: Immunoenzymatic Assay Test Facility: AWL

Processing: Cent. aliquot, refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: 4 Days Test Schedule: Tuesday, Thursday, Sunday Morning Method: Radiobinding Assay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway Insulin Antibody San Juan Capistrano, CA 92690-6130

Clinical Data: Alias Human Insulin Antibody

Patient Preparation: For Clinician Instruction Only Type 1 diabetes is characterized by lymphocytic cell infiltrate of the pancreatic islets. Insulin Antibody is useful in assessing lower titers of autoantibody in patients with diabetes, detecting insulin autoantibody in patients who are ''prediabetic,'' and in detecting autoantibody in patients with other autoimmune disorders. Measurement of GAD-65, ICA-512, and Insulin Antibody is a highly sensitive means to assess risk and predict onset of Type I diabetes. There is a correlation between the number of positive antibodies and the antibody titers versus the severity of the autoimmune process.

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Specimen Stability: Room Temperature: 28 days Refrigerated: 28 days Frozen: 28 days Test Code: CHANTILLY TC 36178 TO SJC TC 36178

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 0.5 mL Serum

TAT: Report available: 3 Days Test Schedule: 3 Days a week Method: Immunoassay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: The free insulin measurement is helpful in interpreting blood sugar concentration Insulin Level Free and its relationship to insulin injections in insulin-treated patients with insulin antibodies. Patient History: last dose.

Patient Preparation: Fasting is required.

Specimen Stability: Room Temperature: 12 hours Refrigerated: 7 days Frozen: 28 days Test Code: SJC TC 36700

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 1mL Serum Alternative Collection: Green NA HEP Volume: 1.0 mL Plasma

TAT: Next day Test Schedule: Mon-Fri Evening Method: Immunoassay Test Facility: Quest, Chantilly

Clinical Data: For diagnosis and monitoring of diabetes and insulin-secreting tumors Insulin Level Total Patient Preparation: Fasting specimen required

specimen Stability: Room temperature: 48 hours Refrigerated: 8 days Frozen: 90 days Test Code: Quest TC 561

Processing: Centrifuge and aliquot serum/plasma into a referred tests aliquot tube. Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes

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Volume: 1.0 mL s Absolute Minimum Volume: 0.5 mL s

TAT: 1 Week Test Schedule: Mon Method: Chemiluminescent Immunometric Assay Insulin-Like Growth Test Facility: AWL Factor I Current name for Somatomedin-C is Insulin Like Growth Factor I (IGF-1).

Processing: Centrifuge. ASAP. Pour. Freeze immediately. Transport: Transport frozen on ice

Preferred Collection/Volume: Blood: Green 3 Sodium Heparin/ 2-3cc Bone Marrow: Bone Marrow Transport Media/ 1cc Lymph Node: Tissue Biopsy Transport Media / 1 cm sq MINIMUM VOLUME 1cc Alternative Collection: Lymph Node: Sterile saline or RPMI Media

TAT: Routine: 14-21 days Test Schedule: Mon-Fri Method: In situ hybridization on interphase cells Test Facility: AWL

Interphase FISH Clinical Data: If ordered in combination with a chromosome study, only 1 specimen is needed. Include clinical indication. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: ** No Gel barrier tubes ** Do not Centrifuge. Hold at Room temperature. Do NOT reject centrifuged specimen; contact department at 503-258-6723 (Tie Line: 35-6723) Transport: Room Temperature.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL s Absolute Minimum: .5mL s

TAT: Report available: Next day Test Schedule: Set up: Monday-Friday Morning Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Intrinsic Factor Clinical Data: Intrinsic Factor, produced by cells lining the stomach, binds vitamin B12 Antibody (cyanocobalamin) to facilitate absorption of the vitamin. Blocking antibody impedes the action of Intrinsic Factor, as observed in approximately half of the patients who develop pernicious anemia. Patient History: Samples should not be collected from a patient who has received Vitamin B12 injection therapy within the last week

Specimen Stability: Room temperature : 7 days Refrigerated: 7 days Frozen : 28 days TC# 568

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

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Preferred Collection/Volume: 24 Urine in Acid Washed Container Volume 10.0 mL urine <>Instructions: Collect without preservatives.

TAT: Report available: 2 days Test Schedule: Monday, Wednesday, Friday Afternoon Method: Inductively Coupled Plasma-Mass Spectrometry (ICP/MS) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Iodine 24 Hr Urine Clinical Data: Iodine is as essential element that is required for thyroid hormone production. The measurement of urinary iodine serves as an index of adequate intake.

Specimen Stability: Room temperature: 10 days Refrigerated: 10 days Frozen: Unacceptable Test Code: CHANTILLY TC 16602

Processing: Mix well and aliquot into a plastic, acid-washed, metal-free leak proof container, Transport: Refrigerate if held.

Preferred Collection/Volume: Dark blue EDTA, metal free Volume: 1.0 mL Plasma Alternative Collection: Dark Blue Serum Volume: 1.0 mL Serum

TAT: Report available: 2 Days Test Schedule: Monday, Wednesday, Friday Afternoon Method: Inductively Coupled Plasma-Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Iodine Total Clinical Data: Iodine is an essential element that is required for thyroid hormone production. The measurement of iodine serves as an index of adequate dietary iodine intake and iodine overload, particularly from iodine-containing drugs such as Amiodarone.

Specimen Stability: Room Temperature: 10 days Refrigerated: 10 days Frozen: Unacceptable Test Code: CHANTILLY TC 16599

Processing: Centrifuge and aliquot into trace metal free aliquot tube Transport: Refrigerate if held.

Preferred Collection/Volume: 24-hour urine collected without preservative Volume: 10 mL

TAT: 4 days Test Schedule: Tuesday, Thursday Afternoon Method: Inductively Coupled Plasma-Mass Spectrometry Iron 24 Hr Urine Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Labeling: Write 24 hour volume on label

Specimen Stability:

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Room Temperature: 4 days Refrigerated: 14 days Frozen: 30 days Test Code: Quest TC 17515

Processing: Mix sample well and aliquot. Note on sample total volume. Transport: Refrigerate if held.

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p Alternative Collection: RED SST or serum acceptable Absolute Minimum Volume: 0.2mL p/s

TAT: 24 Hours Iron Binding Capacity Test Schedule: Daily Total Method: Colorimetric Test Facility: AWL

See panel IRN if iron only STAT testing is requested or suspected overdose

Processing: Cent., Refrigerate if held

Preferred Collection: GRN Li Hep PST Alternative Collection: RED SST or serum acceptable Volume: 0.5mL p Minimum Volume: 0.2mL p/s Iron Level TAT: 24 Hours Test Schedule: Daily

Note: For Iron and TIBC testing, see panel Iron Binding Capacity Total.

Processing: Centrifuge, refrigerate if held over night

Preferred Collection/Volume: Collect Liver Biopsy tissue in a Dark Blue Serum Volume: 5 mg liver biopsy tissue OR Paraffin block is also acceptable. Please note that all tissue is consumed and will not be returned to the client. Alternative Collection: Paraffin-embedded tissue block, room temperature

TAT: 5 Days Test Schedule: Wednesday Morning Method: Inductively Coupled Plasma/Mass Spectrometry Test Facility: Quest Diagnostics Valencia Nichols Institute Iron Level Liver Tissue 27027 Tourney Road Valencia, CA 91355

Clinical Data: Iron Index results between 1.0-1.9 suggest mild iron accumulation (alcoholic liver disease or heterozygous hemochromatosis). Results greater than 1.9 indicate hemochromatosis (transfusion-related iron overload or homozygous hemochromatosis).

Specimen Stability: Liver Biopsy __Room temperature: 28 Days __Refrigerated: 28 Days __Frozen: 56 Days Fixed tissue __Room temperature: 28 Days

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__Refrigerated: 28 Days __Frozen: 60 Days Test Code: Quest TC 3893

Processing: Do Not Centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Report available: 5 Days Test Schedule: Monday-Thursday Night Method: Indirect Immunofluorescence Assay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Type 1 diabetes is characterized by lymphocytic cell infiltrate of the pancreatic islets. Measurement of GAD-65, ICA-512, and Insulin Antibody is a highly sensitive means to Islet Cell Antibody assess risk and predict onset of Type I diabetes. There is a correlation between the number of positive antibodies and the antibody titers versus the severity of the autoimmune process.

Specimen Stability: Room Temperature: 2 days Refrigerated: 2 weeks Frozen: 6 months Instructions: If the screen is positive, the test will reflex to a titer at an additional charge (Test Code: 900314)(CPT 86341). Rejection criterion: Grossly hemolyzed or icteric or lipemic specimens are not acceptable CHANTILLY TC 36741 TO SJC TC 36741

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: Pink EDTA Volume: 5.0 mL Alternative Collection: LAV EDTA RED 10 (do not centrifuge) Absolute Minimum Volume: 1.0 mL Isohemagglutinin Titer Testing performed only at KSMC Blood Bank.

Processing: Do not Cent.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Report available: 9 Days Test Schedule: Tuesday, Thursday Morning Isonicotinic Acid Method: High Performance Liquid Chromatography Hydrazide Level Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave, Suite 200 Cypress, CA 90630

Clinical Data: is a primary antimycobacterial agent for treatment of M. tuberculosis and other mycobacterial diseases.

Patient Preparation: Speciify all antimicrobials being used to treat the patient. Specimens collected just before or within 15 minutes of next dose represent trough levels. Specimens

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obtained within 15-30 minutes after the end of I.V. infusion or 45-60 minutes after an IM injection or 90 minutes after oral intake represent the PEAK level.

Specimen Stability: Room Temperature: unacceptable Refrigerated: unacceptable Frozen: 14 days Test Code: CHANTILLY TC 110792 TO FOCUS TC 51963

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze. Transport: Transport frozen on ice

Preferred Collection/Volume: Do not use alcohol to clean arm. May use chloraprep as alternative. Grey Na Fluor Volume 5.0 mL Whole Blood Alternative Collection: LAV EDTA (whole blood) Volume 5.0 mL whole blood

TAT: Report available: 2 Days Test Schedule: Monday-Friday Afternoon Method: Gas Chromatography Test Facility: Quest Diagnostics Nichols Institute Isopropanol Level 14225 Newbrook Drive Chantilly, VA 20153

Specimen Stability: Room Temperature: 5 Days Refrigerated: 2 Weeks Frozen: 1 Year (-20c) Test Code: CHANTILLY TC 7821

Processing: Do not centrifuge Transport: Refrigerate if held.

Preferred Collection/Volume: Stool collected and placed in Parasite Collection Kit/ to fill line Alternative Collection: n/a

TAT: 1 - 2 Days Test Schedule: Mon - Sat Method: DFA Test Facility: AWL

Clinical Data: n/a Patient History: n/a Isospora Stain Patient Preparation: Stool collected and placed in Parasite collection kit filled to line. Follow directions provided by the laboratory. Labeling: Date and time of collection

Rejection Criteria: Expired transport media Specimen submitted without formalin vial Overfilled collection vial No observable specimen in collection vial

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes

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Volume: 2.0 mL Serum

TAT: Report available: 4 Days Test Schedule: Tuesday, Thursday Morning Method: High Performance Liquid Chromatography Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave, Suite 200 Cypress, CA 90630

Clinical Data: Itraconazole is an antifungal drug useful for the treatment of blastomycosis, histoplasmosis, coccidioidomycosis, sporotrichosis, ringworm, Tinea versicolor, and aspergillosis, as well as for therapy for oral and esophageal candidiasis (thrush). Itraconazole Level Patient Preparation: Specimens collected just before or within 15 minutes of next dose represent trough levels. Specimens obtained within 15-30 minutes after the end of I.V. infusion or 45-60 minutes after an IM injection or 90 minutes after oral intake represent the PEAK level.

Specimen Stability: Room Temperature: unacceptable Refrigerated: unacceptable Frozen: 14 days Test Code: CHANTILLY TC 34973X TO FOCUS TC 51523

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze. Transport: Transport frozen on ice

Preferred Collection: LAV EDTA Whole Blood Alternative Collection: Bone Marrow collected in an LAV EDTA tube Volume: 6.0 mL Whole Blood Minimum Volume: 3.0 mL

TAT: Report available within 3 days Test Schedule: Daily a.m Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 TC# 16539X Jak2 V617F to EXON 12,13 Clinical Data: Myeloproliferative disorders (MPDs) are clonal hematopoietic stem cell malignancies characterized by excessive production of blood cells by hematopoietic precursors. In addition to thrombotic and hemorrhagic complications, leukemic transformation can occur. The main members of MPD are Polycythemia Vera (PV), Essential Thrombocythemia (ET) and Idiopathic Myelofibrosis (MF). The molecular pathogenesis of most MPDs is unknown. This V617F mutation leads to constituitive tyrosine phosphorylation activity that promotes cytokine activity and induces erythrocytosis. The V617F mutation in JAK2 is a dominant gain-of function mutation that contributes to the expansion of the myeloproliferative disorder clone. JAK2 exon 12 mutations define a distinctive myeloproliferative syndrome.

Note: Collect Sunday-Thursday only

Specimen Stability: Room Temperature: 72 hours Refrigerated: 72 hours Frozen: Unacceptable Rejection Criteria: Gross hemolysis, frozen whole blood, frozen bone marrow, clotted whole blood, clotted bone marrow

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Processing: Do not centrifuge or freeze specimen whole blood (preferred) Transport: Refrigerate Note: Transport specimen the same day to maintain sample stability

Preferred Collection: LAV EDTA Whole Blood Alternative Collection: Bone Marrow collected in an LAV EDTA tube Volume: 6.0 mL Whole Blood Minimum Volume: 3.0 mL

TAT: Report available within 3 days Test Schedule: Daily a.m Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Jak2 V617F to EXON Chantilly, VA 20153 12,13 MPL TC# 16538X

Note: Collect Sunday-Thursday only

Specimen Stability: Room Temperature: 72 hours Refrigerated: 72 hours Frozen: Unacceptable Rejection Criteria: Gross hemolysis, frozen whole blood, frozen bone marrow, clotted whole blood, clotted bone marrow

Processing: Do not centrifuge or freeze specimen whole blood (preferred) Transport: Refrigerate Note: Transport specimen the same day to maintain sample stability

Preferred Collection/Volume: Cerebral Spinal Fluid submitted in sterile vial/container Volume: 1mL csf Absolute Minimum: .3mL csf

TAT: Monday through Thursday: 2 days Friday, Saturday: 3 days

Test Schedule: Monday through Friday Test Method: Viral nucleic acid is extracted from the specimen using the MagNA Pure automated instrument (Roche Applied Science). Primers are directed to the large T antigen gene, which is a conserved sequence specific for JC virus (JCV). This assay detects only JCV; it does not detect BK Virus or S-40 (other polyomaviruses). The LightCycler instrument (Roche Applied Science) amplifies and monitors the development of target nucleic acid sequences after the annealing step during PCR cycling. This automated PCR system can rapidly detect amplicon development through stringent air-controlled temperature cycling in capillary cuvettes. The detection of amplified products is based on the fluorescence resonance energy transfer (FRET) principle. For FRET product detection, a hybridization probe with a donor fluorophore, fluorescein, on the 3'-end is excited by an external light source and emits light that is absorbed by a second hybridization probe with an acceptor fluorophore, LC-Red 640, at the 5'-end. The acceptor fluorophore then emits a light of a different wavelength that can be measured with a signal that is proportional to the amount of specific PCR product.(Unpublished Mayo method) Test Location: Mayo Medical Laboratories

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Rochester Useful For: As an aid in diagnosing progressive multifocal leukoencephalopathy due to JC virus Clinical Information: JC virus (JCV), a member of the genus Polyomavirus, is a small nonenveloped DNA- containing virus. Primary infection occurs in early childhood, with a prevalence of >80%. (1) The virus is latent but can reactivate in immunosuppressed patients, especially those with AIDS. JCV is recognized as the etiologic agent of progressive multifocal leukoencephalopathy (PML), a fatal demyelinating disease of the central nervous system.(2,3) Histologic examination of brain biopsy tissue may reveal characteristic pathologic changes localized mainly in oligodendrocytes and astrocytes. Detection of JCV DNA by PCR (target gene, large T antigen) in the cerebrospinal fluid specimens of patients with suspected PML infection has replaced the need for biopsy tissue for laboratory diagnosis.(4) Importantly, the PCR test is specific with no cross-reaction with BK virus (BKV), a closely related polyomavirus JC Virus DNA Qual CSF by PCR Interpretation Detection of JC virus (JCV) DNA supports the clinical diagnosis of progressive multifocal leukoencephalopathy due to JCV Cautions A negative result does not rule out the possibility of JC virus (JCV) infection. This test is not to be used as a diagnostic tool for Creutzfeldt-Jakob disease (CJD). The reference range in cerebrospinal fluid is “negative“ for this assay, although JCV DNA may be detectable in the absence of clinical symptoms in certain patient populations.(6,7) However, this assay is only to be used for patients with appropriate neurological and neuroradiological features of PML, and is not indicated for screening asymptomatic patients

Specimen Stability CSF Refrigerated (preferred) 7 days Frozen 7 days TC# LCJC 88909

Preferred Collection/Volume: REDSST Volume: 1.0mL s Alternative Collection: RED10

TAT: 1-3 days Test Schedule: Monday through Friday Test Method: Multiplex Flow Immunoassay Testing Location: Airport Way Regional Laboratory JO-1 Antibody Clinical Significance Jo-1 Antibody occurs most frequently (31%) in patients with polymyositis, but has also been found in patients with dermatomyositis, and the polymyositis/scleroderma "overlap syndrome" (PM/SCL) or polymyositis/systemic lupus erythematosis "overlap syndrome" (PM/SLE).

Specimen Stability:

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Refrigerated: 7 days Frozen: 30 days Rejection Criteria: Gross hemolysis Gross lipemia

Processing: Centrifuge. Aliquot if collected in RED10. Transportation: Transport to AWL in blue tote

Preferred Collection/Volume: RED10 No Gel Barrier Tubes Volume: 1.0 mL s

TAT: 3 to 4 days Test Schedule: Monday and Thursdays Method: Turbidimetry Kappa, Lambda Lt Test Facility: Airport Way Regional Laboratory Chains, Free w Ratio Sam/Tox Department

Specimen Stability: Refrigerated: 21 days

Processing: Centrifuge and aliquot. Transport: Transport to AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL Whole blood

Test Facility: UC DAVIS Coccidioidomycosis Serology Laboratory 3146 Tupper Hall 1 Shields Avenue Kennedy Disease Gene Davis, CA 95616 Analysis Refrigerate if held overnight. Ship Room Temperature

Processing: Do not Centrifuge. Transport: Send Room Temperature

Preferred Collection: Maternal blood (LV3 or LV5 or EDTA microtainer) Amniotic fluid Vaginal blood Minimum Volume: 1mL Kleihauer-Betke TAT: Stat 4 hrs; routine 24 hours Testing Schedule: 24 hours a day , 7 days a week Test Method: Acid elution Testing Location: Sunnyside Medical Center Rejection Criteria: Cord blood is unacceptable

Processing: NA

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Transport: Transport and store amniotic fluid at room temp.

Preferred Collection/Volume: Submit hair, skin or nail in sterile leakproof container. Submit mouth, tongue, or oral samples on sterile culture swab. Volume: HAIR: Collect 10-12 affected hairs with the base of the shaft intact. Skin: Skin scrapings at the active margin of the lesion. Nail: Clip a generous portion of the affected area and collect material from under the nail.

TAT: 1 day Test Schedule: Daily Method: Microscopy Test Facility: AWL, BVT, CPK, DIV, INT,LVK,NLR,ORC, RKW, SAL, SMC, SKY, SST, TUA, KOH Wet Mount VAN

Clinical Data: n/a Patient History: n/a

Submit hair, skin or nail in sterile leakproof container. Submit mouth, tongue, or oral samples on sterile culture swab.

Processing: Room Temperature Transport: Room Temperature

Preferred Collection/Volume: Preferred Specimen:Formalin fixed paraffin embedded tissue block Alternative Collection: LAV EDTA (whole blood) Volume 6.0 mL Bone Marrow Volume 1.5 mL

TAT: Report available: 5 days Test Schedule: Monday-Friday Morning Method: Polymerase Chain Reaction • Sequencing Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Activating Ras mutations can be found in human malignancies with overall frequency of 15-20%. A high incidence of Ras gene mutations has been reported in 80-90% KRAS Gene Mutation malignant tumors of the pancreas, 30-60% in colon rectal carcinomas, and in 18-30% of hematopoietic neoplasia of myeloid origin. Ras proteins were shown to influence proliferation, Analysis differentiation, transformation, and apoptosis by relaying mitogenic and growth signals into the cytoplasma and the nucleolus. Mutations leading to an amino acid substitution at positions 12,13 and 61 are the most common in naturally occurring neoplasms and are frequent in adenocarcinomas of the pancreas, colon, and certain types of hematological malignancies.

Specimen Stability: Tissue: __Room temperature: Indefinite __Refrigerated: Indefinite __Frozen: Unacceptable Whole blood and bone marrow: __Room temperature: 72 hours __Refrigerated: 72 hours __Frozen: Unacceptable Test Code: CHANTILLY TC 16510

Processing: Submission of formalin fixed paraffin embedded tissue is the preferred sample type. Other sample types listed are acceptable for testing. For submission of paraffin block, another preferred specimen type, tissue source and block ID are required on the requisition form.

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Record sample type, collection time and date onto tube and requisition form.

LBX panel combines with a TSR to give Labotix a secondary target location. This is not a resultable Lab Use TSR test, it is for core lab use only. DO NOT DELETE. CONTACT CLS WITH QUESTIONS

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p Alternative Collection: RED SST or serum acceptable Absolute Minimum Volume: 0.2mL p/s

TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Lactate Dehydrogenase Method: Photometric Rate Test Facility: AWL, SMC

Add-on testing stability: 24 hrs.

Processing: Cent. Refrigerate if held. SPECIAL INSTRUCTIONS: Between the hours of 2200 and 0600, send/cab STATs to SMC

Preferred Collection/Volume: GRN Li Hep 4 Volume: 3.0mL bf Alternative Collection: RED 10 Absolute Minimum Volume: 1.0mL bf

TAT: STAT: 1 Hour Routine: 24 Hours Lactate Dehydrogenase Test Schedule: Daily Body Fluid Method: Photometric Rate Test Facility: AWL, SMC

Use separate LIS requests if both blood and body fluids are ordered.

Processing: Refrigerate if held. CSF: Tube #1 is preferred but any tube may be used

Preferred Collection: GREY Na Fluor Volulme: 1.0 mL plasma ( Absolute Minimum Volume: 0.2 mL plasma) NOTE: Collection tube must be at least half full

TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Method: Colorimetric Lactate Plasma Test Facility: Sunnyside Medical Center and Westside Medical Center

Note: Avoid use of tourniquet. The patient must not clench their hand. If not possible to draw patient without use of tourniquet, draw specimen immediately after application of tourniquet and release it immediately after venipuncture

Processing: Centrifuge and aliquot within 15 minutes of collection. Refrigerate. Transport: Transport on ice.

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p Alternative Collection: RED SST or serum acceptable Absolute Minimum Volume: 0.2mL p/s

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TAT: 24 Hours Test Schedule: Daily Method: Colorimetric- Glucose-quantitation Test Facility: AWL

Patient Preparation: The patient must be scheduled and fasting for 10-14 hours.

Lactose Tolerance (7 Lactose is obtained from the pharmacy. Notify the pharmacy when the patient is scheduled and Specimens) when the patient arrives in the lab. The pharmacy will mix the solution and it requires one hour to dissolve. Adult dose: 50g in 500mL of water. <12yrs:(wgt in lbsx2)/2.2=grams lactose Lactose grams/0.1=mLs of water. DO NOT ADMINISTER MORE THAN 50 GRAMS OR 500 mLs WATER. Draw FBS. Drink Lactose Sol in 10min. Draw 15 min, 30 min, 45 min, 1 hr, 1.5 hr, and 2 hr gluc specimens

Processing: Cent. specimens within 1 hr., Refrigerate.

Preferred Collection: Amniotic Fluid submitted in sterile container Volume: 5.0 mL (Absolute Minimum Volume: 2.0 mL)

TAT: Same day Test Schedule: 7 days weekly 0800-2300 Test Method: Automated cell counter-impedance method Testing Location: Airport Way Regional Laboratory Specimen Stability: Refrigerated 8 days, Room Temp 24 hours

LS ratio is reflexed if the count is >46,000/uL or when specimen integrity criteria is not met (as determined by testing personnel). Testing for LS ratio performed by Lamellar Body Count Legacy Health Systems, Laboratory. Samples will be handled as STAT. w/ Reflex L/S Ratio Processing: Treat specimen as STAT. Transportation: Keep specimen cool. Do not place specimen on ice. Do not freeze. Transport to Airport Way immediately. Rejection Criteria: Specimen received frozen Specimen received on ice Specimen centrifuged Specimen has been filtered Specimen is bloody, clotted, contains meconium, vaginal pool, or is highly viscous

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume 1.0 mL Plasma

TAT: Report available: 2 days Test Schedule: Monday-Friday Morning Lamotrigine Level Method: Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Lamotrigine is an anticonvulsant drug used as adjunctive treatment for refractory partial seizures.

Patient Preparation: Draw 1/2 to 1 hour before next dose at steady state

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Specimen Stability: Room temperature: 48 hours Refrigerated: 5 days Frozen: 14 days Test Code: CHANTILLY TC 22060

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum Alternative Collection: GRN NA Hep Volume 2.0 mL Plasma

TAT: Report available: Same day Test Schedule: Mon, Wed, Fri a.m Method: Electrophoresis Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 LDH Isoenzymes Clinical Data: Test is a diagnostic aid for myocardial infarction; also for malignancies, anemias, and acute liver and muscle injury.

Specimen Stability: Room temperature: 7 days Refrigerated: Unacceptable Frozen: Unacceptable Test Code: CHANTILLY TC 1122

Processing: Centrifuge and aliquot into referred tests aliquot tube as soon as possible. Note if serum or plasma on specimen. Transport: Room temperature

Preferred Collection/Volume: For contamination control during blood collection, use powderless gloves. Tan EDTA Volume 3.0 mL whole blood Alternative Collection: Dark Blue EDTA Volume 3.0 mL whole blood

TAT: Report available: Next Day Test Schedule: Monday-Saturday Morning Method: Atomic Spectroscopy Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Lead Level Clinical Data: Children are especially susceptible to neurologic damage from lead and acute neurologic toxicity may develop without previous symptoms.

Patient Preparation: Patients should refrain from seafood, antacids, vitamins with mineral supplements, and herbal preparations at least 3 days prior to specimen collection.(Refer Patient to Clinician to determine if safe to refrain from dietary supplements)

Specimen Stability: Room Temperature: 5 Days Refrigerated: 14 Days Frozen: 60 Days Test Code: CHANTILLY TC 599

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Processing: Do not centrifuge Transport: Refrigerate if held.

Preferred Collection/Volume: Collection without preservative is preferred 24 Urine in Acid Washed Container Volume 7.0 mL urine Alternative Collection: 24 Urine in Acid Washed Container Volume 7.0 mL urine Preserve with 25 mL 50% Acetic Acid or 30 mL 6N HCl.

TAT: Report available: 3 Days Test Schedule: Monday, Wednesday, Friday Afternoon Method: Inductively Coupled Plasma-Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute Lead Level 24 Hour 14225 Newbrook Drive Urine Chantilly, VA 20153

Specimen Stability: Room Temperature: 2 days Refrigerated: 5 days Frozen: 2 weeks Test Code: CHANTILLY TC 14449

Processing: Mix sample well and aliquot into trace metal free container. Note on sample total volume and preservative used during collection. Transport: Refrigerate if held.

Collection Instructions Preferred Collection/Volume: Legionella (serogroup 1), refrigerated random urine in a plastic screw-capped vial. Urine is stable for 14 days refrigerated Volume: 3mL u Legionella Antigen Urine Specimen Handling Processing: Refrigerate immediately after collection. Transport: Transport on ice. AWL : Refrigerate upon receipt.

Preferred Collection/Volume: Bronchial Wahsings or Sputum Alternative Collection: Lung Biopsy, or fresh(unfixed) tissue, air dried slides, pleural fluid or lower respiratory tract specimen

TAT: Report available: Next Day Test Schedule: Monday-Sunday Morning Method: Direct Immunofluorescence Assay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Legionella DFA Specimen Stability: Room Temperature: 2 hours Refrigerated: 7 Days Frozen: Unacceptable Test Code: CHANTILLY TC 34475

Processing: Rejection criteria: Fixed tissue, Tissue in neutral buffered formalin, Received frozen Transport: Transport on ice

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Preferred Collection/Volume: LAV EDTA Whole Blood Volume 5.0 mL Whole Blood.

TAT: 3 days Test Schedule: Monday through Friday Method: Flow Cytometric Immunophenotyping Includes evaluation of markers CD11a/CD18 and CD11b/CD18. Test Facility: Mayo Medical Laboratories 3050 Superior Drive NW Rochester, MN 55901

Clinical Data: As an aid in the diagnosis of LAD-1 syndrome, primarily in patients <18 years of age CD11a, CD11b, and CD18 phenotypingLeukocyte adhesion deficiency syndrome type 1 (LAD- 1) is an autosomal recessive disorder caused by mutations in the common chain (CD18) of the beta2-integrin family. LAD-1 is clinically characterized by recurrent infections, impaired wound healing, delayed umbilical cord separation, persistent leukocytosis, and recurrent soft tissue and oral infections. Each of the beta2-integrins is a heterodimer composed of an alpha chain (CD11a, CD11b, or CD11c) noncovalently linked to a common beta2-subunit (CD18). The alpha-beta heterodimers of the beta2-integrin family include LFA-1 (CD11a/CD18), Mac-1/CR3 (CD11b/CD18), and p150/95 (CD11c/CD18).(1-4) The CD18 gene, ITGB2, and its product are required for normal expression of the alpha-beta heterodimers. Therefore, defects in CD18 expression lead to either very low or no surface membrane expression of CD11a, CD11b, and CD11c. Severe and moderate forms of LAD-1 exist, differing in the degrees of protein deficiency, which are caused by different ITGB2 mutations. Two relatively distinct clinical phenotypes of LAD-1 have been Leukocyte Adhesion described. Patients with the severe phenotype (<1% of normal expression of CD18 on neutrophils) Deficiency Type 1, characteristically have delayed umbilical stump separation (>30 days), infection of the umbilical CD11a/CD18 and stump (omphalitis), persistent leukocytosis (>15,000/uL) in the absence of overt active infection, and severe destructive gingivitis with periodontitis and associated tooth loss, and alveolar bone CD11b/CD18 Complex resorption. Patients with the moderate phenotype of LAD-1 (1%-30% of normal expression of CD18 Immunotyping, Blood on neutrophils) tend to be diagnosed later in life. Normal umbilical separation, lower risk of life- threatening infections, and longer life expectancy are common in these patients. However, leukocytosis, periodontal disease, and delayed wound healing are still very significant clinical features. Patients with LAD-1 (and other primary immunodeficiency diseases) are unlikely to remain undiagnosed in adulthood. Consequently, this test should not be typically ordered in adults for LAD-1. However, it may be also used to assess immune competence by determining CD18, 11a, and 11b expression.

Patient Preparation: COLLECT MONDAY-THURSDAY AM ONLY!!! SPECIMEN MUST ARRIVE AT REFERRAL LAB WITHIN 24 HOURS OF COLLECTION.

Specimen Stability: Room Temperature: 72 hours Specimen should arrive within 24 hours of draw. Send specimen Monday through Thursday only.Specimen must be received by 10 a.m. on Friday. The specimen will be rejected if >72 hours old. A specimen 48 to 72 hours old will be processed and reported with a disclaimer if expression is absent. Test Code: MAYO TC 81155

Processing: Do Not Centrifuge. Place specimen in a bag marked "Attn: Referred Tests STAT". Specimen must reach Referred Tests by 1400. Core Lab: Take to referred Tests immediately. Transport: Room Temperature

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume 1.0 mL Plasma

TAT: Report available: 2 Days Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Tandem Mass Spectometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

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Clinical Data: Levetiracetam is an anticonvulsant used as adjunct therapy to treat adult partial seizures. As multiple anticonvulsants are administered, it is important to monitor its level to (1) optimize therapy, (2)assure compliance, and (3) to avoid toxicity.

Patient Preparation: Collect trough immediately prior to next dose.

Specimen Stability: Levetiracetam Level, Room Temperature: 14 days HPLC/MS/MS Refrigerated: 28 days Frozen: 60 days Test Code: CHANTILLY TC 15142

Processing: Centrifuge and aliquot into Referred Tests aliquot tube.Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 2 - 4 days Test Schedule: Monday-Friday Method: Electrochemiluminescent (ECL) Technology Test Facility: Esoterix 4301 Lost Hills Road LH Pediatric Calabasas Hills, CA 91301

Store and ship refrigerated or frozen in plastic vial. Test Code: ESOTERIX TC 500234

Processing: Centrifuge and aliquot into Referred Tests aliquot tube within one hour of collection. Transport: Transport on ice

Preferred Collection/Volume: RED SST Volume 2.0 mL Serum.

TAT: 24 hours Test Schedule: 7 days/week Method: EIA Test Facility: Legacy Central Lab 1225 NE Second Ave Portland, OR 97232 Lidocaine Level Patient Preparation: PREFERRED DRAW: 12 hours after initial dose, then every 24 hrs. For cardiac or hepatic dysfunction; draw every 12 hours.

LEGACY CS: 503-413-1234. NO TEST CODE

Processing: Centrifuge specimen. Transport: Transport on ice

Preferred Collection: GRN Li Hep PST Alternative Collection: RED SST RED 10

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Volume: 0.5mL plasma/serum (Absolute Minimum Volume: 0.2 mL plasma/serum)

TAT: Stat:1 Hour Routine: 24 Hours after arrival in lab Lipase Level Testing Schedule: Daily Method: Colorimetric Testing Location: BVN, CPK, INT, LVK, NLR, SKY, SMC, WMC

Processing: Centrifuge. Refrigerate if held

Preferred Collection/Volume: GRN Li Hep PST Volume: 1.0mL p Alternative Collection: RED SST LAV EDTA

TAT: 24 Hours Test Schedule: Daily Lipid Panel Fasting Method: See Individual Assays Test Facility: AWL

Outpatients must fast 10 hours or more prior to collection. If triglyceride is greater than 400 mg/dL, a direct LDL cholesterol will be reflexed. Panel includes: cholesterol, triglycerides, HDL cholesterol and calculated LDL

Processing: Centrifuge. Refrigerate if held

Preferred Collection/Volume: GRN Li Hep PST Volume: 1.0 mL p Alternative Collection: RED SST LAV EDTA

TAT: 24 Hours Test Schedule: Daily Method: See Individual Assays Lipid Panel, Nonfasting Test Facility: AWL

Clinical Data: Includes Cholesterol, NonFasting Triglycerides, HDL cholesterol and Direct LDL cholesterol. This panel is used for lipid studies if the patient is NOT fasting or is fasting less than 10 hours

Processing: Centrifuge. Refrigerate if held

Preferred Collection/Volume: Avoid hemolysis. Grossly lipemic specimens will be rejected. RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume 1.0 mL Plasma

TAT: Report available: Next Day Lipoprotein (a) Test Schedule: Monday-Saturday Morning, Night, Sunday Night Method: Immunoturbidimetric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Elevated concentrations of Lp(a) are associated with increased risk of coronary artery disease. See Lipoprotein (a) and LDL Subparticles in the Markers of Lipidemia chapter, Interpretive Information section.

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Specimen Stability: Room Temperature: 7 days Refrigerated: 14 days Frozen: 90 days (-20c) Avoid hemolysis. Grossly lipemic specimens will be rejected. Test Code: CHANTILLY TC 29702

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA No Gel Barrier Volume: 1.0mL Alternative Collection: RED 10 Absolute Minimum Volume: 0.5mL s/p

TAT: STAT: 1 Hour Routine: 24 Hours Lithium Level Test Schedule: Daily Method: Spectrophotometric Test Facility: AWL, SMC

Preferred draw time: 12 hrs. post evening dose. Separate plasma or serum within 4 hours of collection

Processing: Cent. Aliquot. Refrigerate

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Tuessday, Thursday, a.m Method: Enzyme Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Liver Kidney Clinical Data: The presence of LKM-1 antibodies can be used in conjunction with clinical Microsomal Antibody findings and other laboratory tests to aid in the diagnosis of autoimmune liver diseases such as autoimmune hepatitis (AIH-2).

Specimen Stability: Room temperature: 4 days Refrigerated: 14 days Frozen: 30 days Test Code: CHANTILLY TC 71902

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum Alternative Collection: LAV EDTA Volume 2.0 mL Plasma

TAT: Report available: 4 Days Test Schedule: Tuesday, Thursday Morning

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Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Specimen Stability: Room Temperature: 72 hours Lorazepam Level Refrigerated (cold packs): 72 hours Frozen: Unacceptable Test Code: CHANTILLY TC 0202

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Note if serum or plasma on specimen. Transport: Refrigerate if held

Preferred Collection/Volume: Random Urine in AP99 Volume 100.0 mL Urine

TAT: Report available: Next Day Test Schedule: Monday-Saturday All Shifts Method: High Performance Liquid Chromatography Radioimmunoassay Test Facility: Quest Diagnostics Nichols Institute LSD Urine Qualitative, 14225 Newbrook Drive EIA Chantilly, VA 20153 Specimen Stability: Room Temperature: 72 Hours Refrigerated: 14 Days Frozen: 30 Days Test Code: CHANTILLY TC 37025

Transport: Refrigerate if held

Preferred Collection/Volume: Random Urine in AP99 Volume 20.0 mL Urine WRAP IN FOIL TO PROTECT FROM LIGHT.

TAT: Report available: 7 Days Test Schedule: Wednesday Morning Method: Gas Chromatography Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 LSD Urine Quant Specimen Stability: Room Temperature: 3 days Refrigerated: 14 days Frozen: 1 month Protect specimen from light (wrap in foil). Test Code: CHANTILLY TC 19917

Processing: Foil wrap samples to protect from light. Transport refrigerated Transport: Refrigerate if held

Preferred Collection/Volume: 3 BLUE CIT Volume: 1.0 mL Plasma x4 Tubes must be full.

TAT: run 1-2 times per week. Test Schedule: M-F, batched Method: clotting assay

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Test Facility: OHSU Laboratories Homeostasis and Thrombosis 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Send specimen to the H&T Lab, Dillehunt Hall, Room 3050 Lupus Anticoagulant Test Code: OHSU TC 068-0742

Processing: Centrifuge immediately and aliquot plasma into 2 aliquot tubes using a plastic pipette. Do NOT use filter to aliquot. Avoid buffy coat layer. Re-centrifuge the aliquots and re-aliquot using plastic pipette into 4 aliquot tubes (1.0 ml each). Freeze Transport: Transport frozen on ice, do not allow to thaw

Preferred Collection/Volume: RED 10 Volume: 1.5 mLs serum Alternative Collection: GRN Li Hep GRN Na Hep Absolute Minimum Volume: 1.0 mL s/p

Luteinizing Hormone TAT: 24 Hours Test Schedule: Mon-SAT Method: Immunoassay Test Facility: AWL

Processing: Centrifuge and aliquot. Refrigerate if held.

Preferred Collection/Volume: Gross hemolysis or gross lipemia is unacceptable. RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning Method: Immunoassay Western Blot Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Lyme disease is transmitted by a tick vector carrying Borrelia burgdorferi. Specimens are screened by the EIA (total antibody) test and all positive and indeterminate screen Lyme Disease Antibody results are confirmed by retesting the specimen, using the IgG and IgM Western Blot procedures.

Patient Preparation: Positive EIA results will be confirmed by Lyme IgG and IgM Western Blot at an additional charge.

Specimen Stability: Room Temperature: 4 days Refrigerated: 7 days Frozen: 30 days Rejection criteria: Gross hemolysis or gross lipemia is unacceptable. Test Code: CHANTILLY TC 6646

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: Must reach OHSU w/in 24 hrs of draw and by 1400 on Fridays. Send CBC and Diff results with specimen to OHSU if sent directly from collection site. x 2 LAV EDTA Volume: 5.0 mL whole blood each tube

Test Schedule: COLLECT SUNDAY-THURSDAY ONLY Test Facility: OHSU, Dillehunt Hall, Rm 3030, 494-7383

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Clinical Data: Includes CD4, 8, 3, 56 and 16 (K cells), 19 (B cells), Total T cells and % of total Lymphs.

Send CBC & Diff results with specimen to OHSU . Room Temp Lymph Panel Abs Test Code: OHSU TC087-6051

Processing: Do Not Centrifuge. TO SEND Directly to OHSU: Send CBC & Diff results with specimen to OHSU with a specimen transport form. Transport: Send Room temperature.

Preferred Collection/Volume: x 2 LAV EDTA Volume: 5.0 mL whole blood each tube Must reach OHSU w/in 24 hrs of draw and by 1400 on Fridays. Send CBC and Diff results with specimen to OHSU if sent directly from collection site.

Test Schedule: Performed Monday-Friday, 0730-1530 Test Facility: OHSU, Dillehunt Hall, Rm 3030, 494-7384

Lymph Panel w LK Send CBC & Diff results with specimen to OHSU . Room Temp Test Code: OHSU TC087-6009

Processing: Do Not Centrifuge. TO SEND Directly to OHSU: Send CBC & Diff results with specimen to OHSU with a specimen transport form. Include patient Name, HRN, DOB, and TC# 6009 on form. Place an order note in Order Result Viewer stating specimen sent directly to OHSU. Send one copy of form with specimen to OHSU and one copy to AWL. Transport: Send Room temperature.

Preferred Collection/Volume: COLLECT MONDAY-THURSDAY AM ONLY. Must reach AWL Referred Tests by 1300. X2 GREEN NA HEP 10 Volume 20.0 mL Alternative Collection: Specimen Volume by Patient Age: <3 months: 1.0 mL 3-24 months: 3.0 mL 25 months-18 years: 5.0 mL

TAT: 11 days Test Schedule: Monday through Friday Method: Flow Cytometry Test Facility: Mayo Medical Laboratories 3050 Superior Drive NW Rochester, MN 55901

Clinical Data: Determining impaired T-cell function by culturing human peripheral blood mononuclear cells (PBMCs) in vitro with recall antigens, including Candida albicans (CA) and tetanus toxoid (TT) has been part of the diagnostic immunology repertoire for many years.(1,2) The widely used method for assessing lymphocyte proliferation to antigens has hitherto been the measurement of 3H-thymidine incorporated into the DNA of proliferating cells. The disadvantages with the 3H-thymidine method of lymphocyte proliferation are: 1. The technique is cumbersome due to the use of radioactivity 2. It does not allow discrimination of responding cell populations in response to stimulation 3. It does not provide any information on contribution of activation-induced cell death to the interpretation of the final result Further, decreased lymphocyte proliferation could be due to several factors, including overall diminution of T-cell proliferation or decrease in proliferation of only a subset of T cells, or an apparent decrease in total lymphocyte proliferation due to T-cell lymphopenia and underrepresentation of T cells in the PBMC pool. None of these can be discriminated by the thymidine uptake assay, but can be assessed by flow cytometry, which uses antibodies to identify specific responder cell populations. Cell viability can also be measured within the same assay without requiring additional cell manipulation or sample. Antigens, like CA and TT, have been widely used to measure antigen-specific recall (anamnestic) T-cell responses when assessing cellular immunity. In fact, it may be more revealing about cellular immune compromise than assessing the response of lymphocytes to mitogens because the latter

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can induce T-cell proliferative responses even if those T cells are incapable of responding adequately to antigenic (physiologic) stimuli. Therefore, abnormal T-cell responses to antigens are considered a diagnostically more sensitive, but less specific, test of aberrant T-cell function.(2) Antigens used in recall assays measure the ability of T cells bearing specific T-cell receptors (TCRs) to respond to such antigens when processed and presented by antigen-presenting cells. The antigens used for assessment of the cellular immune response are selected to represent antigens, seen by a majority of the population, either through natural exposure (CA) or as a result of vaccination (TT). For this assay, we use a method that directly measures the S-phase proliferation of lymphocytes through the use of click chemistry. Cell viability, apoptosis, and death can also be measured by flow cytometry using 7-AAD and Annexin V. The Click-iT-EdU assay has already been shown to be an acceptable alternative to the 3H-thymidine assay for measuring lymphocyte/T-cell proliferation.(5) The degree of impairment of antigen-specific T-cell responses can vary depending on the nature of the cellular immune compromise. For example, some, but not all, patients with partial DiGeorge syndrome, a primary cellular immunodeficiency, have been reported to have either decreased or absent T-cell responses to CA and TT.(6) Similarly, relative immune compromise, especially to TT, has been reported in children with vitamin A deficiency, but the measurements have been largely of the humoral immune response. Since this requires participation of the cellular immune compartment, it can be postulated that there could be a potential impairment of antigen-specific T- cell responses as well.(7) Lymphocyte Ag Proliferation Patient Preparation: If both antigens and mitogens are desired, order LPMAF/60593 Lymphocyte Proliferation Panel for Mitogens and Antigens. For serial monitoring, it is recommend that specimen draws be performed at the same time of day.

Specimen Stability: Room Temperature: 48 hours Refrigerated: Unacceptable Frozen: Unacceptable. Specimen must arrive within 24 hours of draw and by 10 a.m. on Friday. Send specimen Sunday through Thursday only. Draw and package specimen as close to shipping time as possible. Ship specimen priority overnight in an Ambient Mailer-Critical Specimens Only (Supply T668). Date and time of draw and ordering physician name and phone number are required. Specify "Antigen" to differentiate from "Mitogen" testing. This information is required. Test Code: MAYO TC 60592

Processing: Do not centrifuge. Must reach AWL Referred Tests by 1300 on day of collection. Place specimen in bag marked "Referred Tests STAT". Transport: Send r oom temperature

Preferred Collection/Volume: GREEN NA HEP 10 Whole Blood Volume: 10.0 mL Alternative Collection: Specimen Volume by Patient Age: <3 months: 1.0 mL 3 months-5 years: 2.0 mL 6-18 years: 3.0 mL

TAT: 8 days Test Schedule: Monday through Friday Do not send specimen after Thursday. Method: Flow Cytometry Test Facility: Mayo Medical Laboratories 3050 Superior Drive NW Rochester, MN 55901

Clinical Data: The method of determining impaired T-cell function by culturing human peripheral blood mononuclear cells (PBMCs) in vitro with mitogenic plant lectins (mitogens) such as phytohemagglutinin (PHA) and pokeweed mitogen (PWM) has been part of the diagnostic immunology repertoire for many years.(1,2) The widely used method for assessing lymphocyte proliferation has hitherto been the measurement of 3H-thymidine incorporated into the DNA of proliferating cells. The disadvantages with the 3H-thymidine method of lymphocyte proliferation are: 1. The technique is cumbersome due to the use of radioactivity 2. It does not allow discrimination of responding cell populations in response to stimulation 3. It does not provide any information on contribution of activation-induced cell death to the interpretation of the final result Further, decreased lymphocyte proliferation could be due to several factors, including overall diminution of T-cell proliferation or decrease in proliferation of only a subset of T cells, or an

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apparent decrease in total lymphocyte proliferation due to T-cell lymphopenia and under- representation of T cells in the PBMC pool. None of these can be discriminated by the thymidine uptake assay, but can be assessed by flow cytometry, which uses antibodies to identify specific responder cell populations. Cell viability can also be measured within the same assay without requiring additional cell manipulation or specimen. Mitogens are very potent stimulators of T-cell activation and proliferation independent of their antigenic specificity.(3) It has been suggested that mitogens can induce T-cell proliferative responses even if they are incapable of responding adequately to antigenic (physiologic) stimuli. Therefore, abnormal T-cell responses to mitogens are considered a diagnostically less sensitive but more specific test of aberrant T-cell function. Lectin mitogens have been shown to bind the T-cell receptor, which is glycosylated through its carbohydrate moiety, thereby activating quiescent T cells. Mitogenic stimulation has been shown to increase intracellular calcium (CA++) in T cells, which is absolutely essential for T-cell proliferation. While PHA is a strong T-cell mitogen, PWM is a weak T-cell mitogen, but it also induces B-cell activation and proliferation as well. For this assay, we use a method that directly measures the S-phase proliferation of lymphocytes through the use of Click chemistry. In the Invitrogen Click-iT-EdU assay, the Click chemistry has been adapted to measure cell proliferation through direct detection of nucleotide incorporation. In the assay, an alkyne-modified nucleoside is supplied in cell-growth media for a defined time period and is incorporated within cells. The cells are subsequently fixed, permeabilized, and reacted with a dye-labeled azide, catalyzed by copper. A covalent bond is formed between the dye and the incorporated nucleotide, and the fluorescent signal is then measured by flow cytometry.(4) Specific proliferating cell populations can be visualized by the addition of cell-specific antibodies. Cell viability, apoptosis, and death can also be measured by flow cytometry using 7-AAD and Annexin V. The Click-iT-EdU assay has already been shown to be an acceptable alternative to the 3H- thymidine assay for measuring lymphocyte/T-cell proliferation.(5) The absolute counts of lymphocyte subsets are known to be influenced by a variety of biological factors, including hormones, the environment, and temperature. The studies on diurnal (circadian) variation in lymphocyte counts have demonstrated progressive increase in CD4 T-cell count Lymphocyte Mitogen throughout the day, while CD8 T cells and CD19+ B cells increase between 8:30 am and noon, with Proliferation no change between noon and afternoon. Natural killer (NK)-cell counts, on the other hand, are constant throughout the day. Circadian variations in circulating T-cell counts have been shown to be negatively correlated with plasma cortisol concentration. In fact, cortisol and catecholamine concentrations control distribution and, therefore, numbers of naive versus effector CD4 and CD8 T cells. It is generally accepted that lower CD4 T-cell counts are seen in the morning compared with the evening, and during summer compared to winter. These data, therefore, indicate that timing, and consistency in timing, of blood collection is critical when serially monitoring patients for lymphocyte subsets

Patient Preparation: Clinician Instructions ONLY: If both antigens and mitogens are desired, order LPMAF/60593 Lymphocyte Proliferation Panel for Mitogens and Antigens. For serial monitoring, it is recommend that specimen draws be performed at the same time of day.

Specimen Stability: Room Temperature: 48 hours Refrigerated: Unacceptable Frozen: Unacceptable. Specimen must arrive within 24 hours of draw and by 10 a.m. on Friday. Send specimen Sunday through Thursday only. Draw and package specimen as close to shipping time as possible. Ship specimen priority overnight in an Ambient Mailer-Critical Specimens Only (Supply T668). Date and time of draw and ordering physician name and phone number are required. Specify"Mitogen" to differentiate from "Antigen" testing. This information is required. Test Code: MAYO TC 60591

Processing: Do not centrifuge. Must reach AWL Referred Tests by 1300 on day of collection. Place specimen in bag marked "Referred Tests STAT". Transport: Room temperature

Preferred Collection/Volume: RED SST Volume: 2.0 mL s Absolute Minimum Volume: .5 mL s

TAT: Report available: Next Day Test Schedule: Monday, Thursday Afternoon Method: Enzymatic Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive

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Chantilly, VA 20153

Specimen Stability: Room Temperature: 8 hours Refrigerated: 1 week Frozen: 1 month Lysozyme TC#: 5082

Processing: Centrifuge within 1 hour of collection. Freeze. Transport: Transport frozen on ice

Preferred Collection/Volume: Random Urine in AP99 Volume 20.0 mL Urine

TAT: Report available: Next Day Test Schedule: Monday, Thursday Afternoon Method: Enzymatic Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Lysozyme Urine Specimen Stability: Room Temperature: Unacceptable Refrigerated: 1 week Frozen: 1 month Test Code: CHANTILLY TC 5087

Processing: Centrifuge urine immediately after collection. Aliquot fluid (without disturbing particulate pellet) to a new AP99 container and freeze. Transport: Transport frozen on ice

Preferred Collection/Volume: Stool specimen submitted in leak proof container Volume: All stool volumes are acceptable

Testing Location: Center for Health Research Study Testing not performed by Kaiser Permanente NW Regional Laboratory

This is a Center for Health Research study.

Specimen Stability: No requirements Maage Study Rejection Criteria: None Store samples at refrigerated temperature once received at AWL in bin labeled "CHR Stool Study" Study samples will be transported to CHR via KP courier 18 at 0854 each week day.

Processing: Do not place LIS label over top of CHR generated label. Place each sample in ziplock bag for transport. Transportation: Transport on ice to Airport Way Regional Laboratory in blue tote.

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p Alternative Collection: RED SST or serum Magnesium Level Absolute Minimum Volume: 0.2mL p/s

TAT: STAT: 1 Hour Routine: 24 Hours

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Test Schedule: Daily Method: Colorimetric Test Facility: AWL, INT, SMC, LVK

Processing: Cent. Refrigerate if held

Preferred Collection/Volume: 24 hour urine colllected with 25 mL 6N HCL to maintain a pH below 3. Refrigerate during and after collection. Acceptable to add preservative after collection Alternative Preservative: Glacial acetic acid or 10 g boric acid Volume: 10mL u

TAT: Report available: Next Day Test Schedule: Monday, Wednesday, Friday Morning, Afternoon Method: Atomic Absorption Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Magnesium Level 24 Hour Urine Clinical Significance Urinary magnesium concentrations often decline before serum concentrations. Adverse effects, such as cardiac arrhythmias, can be observed while serum concentrations are within range.

Specimen Stability: Room Temperature: 4 Days Refrigerated: 7 Days Frozen 28 Days TC#: 14459

Processing: Mix sample well and aliquot. Note on sample total volume. Transport: Tranport to AWL.

Preferred Collection/Volume: LV3 or LV5 or EDTA microtainer Alternative Collection: n/a

TAT: Stat within 3 hrs of specimen receipt at AWL; Routine within 24 hrs Test Schedule: Any day (see ‘note’ under Referred Test/Additional) Test Method: Microscopic examination of thick and thin smears. Test Facility: AWL, Wet/Dry microscopy

Clinical Data: n/a Patient History: n/a

Malaria and Blood Patient Preparation: n/a Parasites Smear Labeling: n/a

Note: One negative result does not rule out the possibility of parasitic blood infection. If parasitic blood infection is strongly suspected, test should be performed at least once per day for 3 consecutive days Note: Wet/Dry microscopy is opened 7:30am – 12:00 midnight on weekdays, and 3:30pm to 12:00 (midnight) on weekends. If a clinician wants the results urgently and it’s outside the W/D microscopy operating hours, cab specimen to Legacy Emanuel.

Processing: Handle microtainer ASAP and test on-site when possible Transport: Transport at refrigerator temperature when sending to AWL.

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Preferred Collection/Volume: DARK BLUE EDTA Volume 4.0 mL Whole Blood. To avoid contamination use powderless gloves.

TAT: Reports available: 3-4 days Test Schedule: 5 days a week, p.m Method: Inductively Coupled Plasma/Mass Spectrometry (ICP/MS) Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Manganese is an essential trace metal. Toxicity that can result from excessive exposure can cause serious organ damage. Manganese can be measured in a variety of body Manganese Level fluids and tissues. Patient Preparation: Patient should refrain from taking manganese supplements at least three days before collection of sample (Refer Patient to Clinician to determine if safe to refrain from dietary supplements)

Specimen Stability: Room temperature: 5 days Refrigerated: 14 days Frozen: Unacceptable Test Code: SJC TC 626X

Processing: Do not centrifuge. Do not aliquot. Transport: Refrigerate if held

Preferred Collection/Volume: Minimum of 1 mL EDTA whole blood or two peripheral blood smears. Alternative Collection: Citrate tube is used when PLT clumping

TAT: Routine same day; stats within an hour Test Schedule: Any day Test Method: Microscopic examination of Wight’s stained blood smear. Test Facility: All KPNW labs with hematology testing.

Clinical Data: Must be added to or ordered with a CBC or ABC. Manual Diff Patient History: n/a

Patient Preparation: n/a Labeling: n/a

If platelets are clumping in the EDTA specimen, collect a blue top citrate tube. Note : Sodium citrate prevents platelets from clumping in majority of cases.

Processing: n/a Transport: n/a

Preferred Collection/Volume: RED SST Volume: 7mL s See WI 2.2-08-213 BLOOD: (1st and 2nd Trimester NTD Screens) for complete instructions.

Test Facility: NTD Labs, from PerkinElmer 80 Ruland Rd. Suite 1, Melville,NY 11747

Clinical Data: The Quad Screen | Fß is performed in the second trimester of pregnancy, between 15 weeks and 21 weeks, 6 days gestation. The mother's serum is collected and sent to NTD Labs for analysis. This test analyzes 4 biomarkers found in the mother's serum - alpha- fetoprotein (AFP), free beta human chorionic gonadotrophin (hCG), unconjugated estriol, and

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inhibin-A. This test is essentially the same as Part 2 of the Sequential Screen | Fß. This test is suggested for patients who present too late in their pregnancy to receive first trimester screening.The Quad Screen | Fß detects 75% of Down syndrome cases at a 5% false positive rate, and detects 75% of trisomy 18 cases at a 0.3% false positive rate. This test detects 90% of cases of open spina bifida and 98% of cases of anencephaly.

Patient Preparation: Patient will present for collection at second trimester of pregnancy, between Maternal Quad Screen 15 weeks and 21 weeks, 6 days gestation. 2nd Trimester (AFP, UE3, Free bHCG, Specimen Stability: Inhibin A) Room Temp: OK Refrigerated: OK Frozen: Aliquot ONLY OK

Processing: Centrifuge sample. Do not aliquot. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 2.0 mL Plasma Alternative Collection: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: 3 Days Test Schedule: Tuesday, Thursday Afternoon Method: High Performance Liquid Chromatography Test Facility: National Medical Services 3701 Welsh Rd Mephenytoin and Willow Grove, PA 19090 Normephenytoin Levels Note: Test performed at NMS via Quest Chantilly Specimen Stability: Room Temperature: 14 days Refrigerated: 14 days Frozen: 13 months (-20c ) Test Code: Quest TC 290

Processing: Centrifuge and aliquot into a Referred Test aliquot tube.Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: GRN Li Hep 4 NO Gel Barrier Tubes Volume: 0.5 mL p Alternative Collection: RED 10 Absolute Minimum Volume: 0.2 mL p\s

TAT: STAT: 1 Hour Routine: 24 Hour Routine Test Schedule: Daily Mephobarbital Level Method: Immunoassay Test Facility: SMC

Patient History: SAME AS PHENOBARB.

Mephobarbital is metabolized to phenobarbital. Preferred draw time: trough - immediately before next dose

Processing: Cent.,aliquot, refrigerate

Preferred Collection/Volume: Dark Blue EDTA Volume 4.0 mL Whole Blood.

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To avoid contamination use powderless gloves.

TAT: 3 days Test Schedule: Monday, Wednesday, Friday Afternoon Method: Inductively Coupled Plasma/Mass Spectrometry (ICP/MS) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: The primary clinical utility of blood mercury is the determination of abnormal exposures seen at levels over 20 µg/L. Mercury is absorbed via the respiratory tract (mercury vapors), skin, and gastrointestinal tract. Mercury poisoning can cause kidney damage. The chronic effect of mercury poisoning includes inflammation of mouth and gums, loosening of the teeth, Mercury Level kidney damage, nervousness, depression, and spasms.

Patient Preparation: Patient should refrain from eating predatory fish such as swordfish, tuna, and shark at least three days prior to specimen collection.

Specimen Stability: Room Temperature: 5 Days Refrigerated: 7 Days Frozen: Unacceptable Test Code: CHANTILLY TC 1971

Processing: Do not centrifuge. Do not aliquot. Transport: Refrigerate if held

Preferred Collection/Volume: 24 Urine in Acid Washed Container (Without Preservative) Volume 7.0 mL urine

TAT: Report available: 3 Days Test Schedule: Monday, Wednesday, Friday Afternoon Method: Inductively Coupled Plasma-Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Mercury Level 24 Hour Patient Preparation: Patient should refrain from eating predatory fish, such as swordfish, tuna, Urine and shark, at least 3 days before specimen collection. Specimen Stability: Room Temperature: 2 Days Refrigerated: 5 Days Frozen: 2 Weeks Test Code: CHANTILLY TC 14453

Processing: Mix sample well and aliquot into acid washed container. Note total volume on sample . Transport: Refrigerate if held

Preferred Collection/Volume: Draw specimen in a pre-chilled LAV EDTA. The whole blood sample should be kept on wet ice until centrifuged (preferably at 4° C) to separate the plasma within 2 hours of venipuncture. LAV EDTA Prechilled Volume: 2.5 mL plasma

TAT: Report available: 2 days Test Schedule: Set up: Monday-Friday Morning Method: Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

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Clinical Data: Normetanephrine (NM) and (MN) are the extra-neuronal catechol- o-methyl transferase (COMT) metabolites of the catecholamines norepinephrine and epinephrine, respectively. Measurement of plasma is more sensitive (but may be less specific) than measurement of catecholamines for the detection of pheochromocytoma. Proper interpretation of results requires awareness of recent medication/drug history (e.g., antyhypertensive agents, alcohol, cocaine) and other pre-analytical factors (e.g., stress, severe congestive heart failure, myocardial infarction) that influence release of catecholamines and metanephrines.

Patient Preparation: Patient should avoid alcohol, coffee, tea, tobacco and strenuous exercise prior to collection. Overnight fasting is preferred. Patients should be relaxed in either a supine or Metanephrines upright position before blood is drawn. Specimen Stability: Room temperature: 4 Hours Refrigerated: 14 Days Frozen: 14 Days Test Code: CHANTILLY TC 19548

Processing: Centrifuge within two hours of collection and aliquot into Referred Tests aliquot tube. Transport: Tranport on ice.

Preferred Collection/Volume: 24 hour urine c ollected without preservative. Patient should refrigerate collection container during collection. Volume: 5.0 mL u

TAT: Report available: 3 Days Test Schedule: Monday-Friday Morning Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Test is useful in the evaluation of pheochromocytoma.

Patient Preparation: Clinician Instructions ONLY: It is preferable for the patient to be off medications for three days Metanephrines 24 Hour before collection. Patient should avoid tobacco, tea, coffee for three days before specimen Urine collection. Common antihypertensives (diuretics, ACE inhibitors, calcium channel blockers, alpha and beta blockers) cause minimal or no interference. Medications which are alpha agonists (Aldomet), alpha blockers (Dibenzyline) should be avoided 18-24 hours before specimen collection.

Specimen Stability: Room Temperature: 7 days Refrigerated: 8 days Frozen: 30 days Record 24-hour urine volume and patient's age on test request form and sample TC# 14962X

Processing: Mix sample well and aliquot. Adjust pH to < 3 with 6N HCL. Note on sample total volume and age of patient. Transport: Refrigerate if held

Preferred Collection/Volume: AP99 Random urine Volume: 50 mL u Absolute Minimum Volume: 10 mL u Methadone Screen TAT: 24 Hours Test Schedule: Daily Method: Immunoassay Test Facility: AWL

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Included in drug screen panels DSB and DSC.

Processing: Refrigerate if held overnight

Preferred Collection/Volume: AP99 Random urine Volume: 50 mL u Absolute Minimum Volume: 10 mL u

TAT: 24 Hours Test Schedule: Mon - Sat Methadone Urine Method: GC/MS Confirm Test Facility: AWL

To order a drug screen see panels MES, DSB, or DSC

Processing: Refrigerate if held overnight

Preferred Collection/Volume: Grey Na Fluor- whole blood Volume: 4.0 mL Whole Blood Use NON alcohol disinfection for blood specimen collection.

TAT: 24 hours Test Schedule: 7 days/week Method: GC Test Facility: Legacy Central Lab Methanol Level 1225 NE Second Ave Portland, OR 97232

LEGACY CS: 503-413-1234. NO TEST CODE

Processing: Do not centrifuge. Do not aliquot Transport: Tranport on ice.

Preferred Collection/Volume: Random Urine in AP99 Volume: 5.0 mL Urine

TAT: Report available: Next Day Test Schedule: Monday-Sunday Morning Method: Gas Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Methanol Urine Chantilly, VA 20153 Specimen Stability: Room Temperature: 5 Days Refrigerated: 2 Weeks Frozen: 1 Month (-20c) Test Code: CHANTILLY TC 4307

Transport: Refrigerate if held

Preferred Collection/Volume: Red 10 Volume: 2 mL Serum Alternative Collection: GRN LI or Na Volume: 2 mL Plasma LAV EDTA Volume: 2 mL Plasma

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TAT: 3 days Test Schedule: Monday Method: Gas Chromatography (GC) Test Facility: NMS LABS 3701 Welsh Road Willow Grove, PA 19090

Clinical Data: Therapeutic Drug Monitoring

Metharbital Specimen Stability: Room Temperature: 14 day(s) Refrigerated: 14 day(s) Frozen (-20 °C): 12 month(s) Test Code: NMS TC 2860SP

Processing: Centrifuge and aliquot into a Referred Test aliquot tube. Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection: DkGrn Li Hep Volume: 4.0mL wb Alternative Collection: Grn Na Hep or Heparinized syringe/capillary tube Absolute Minimum Volume: 0.5mL wb

TAT: STAT: 1 Hour Methemoglobin Testing Schedule: Daily Quantitative Method: Co-oximetry Testing Location: Sunnyside Medical Center and Westside Medical Center

Note: Stable 1 hr in filled, well capped tube. If drawn in syringe or capillary tube expel air bubbles immediately after draw. Cap syringe securely and mix. Rubber cap capillary tubes. Heparinized syringe/capillary tube (0.5 mL min.) Test can only be drawn at Sunnyside Medical Center or Westside Medical Center.

Processing: Do not centrifuge Transport: Transport specimen at room temperature to testing location

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume:1.0 mL s Alternative Collection: GRN Na Hep 4 LAV EDTA Absolute Minimum Volume: 0.5 mL s/p

TAT: STAT: 1 Hour Routine: 24 Hours Methotrexate Level Test Schedule: Daily Method: Fluorenscence Polarization Immunoassay Test Facility: AWL STAT collected between 2200 - 0600 should be tested at Legacy

Protect from light. Preferred draw time: 48 hrs. post high dose therapy

Processing: Centrifuge and Aliquot. Cover with foil. Transport: Refrigerate if held

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Preferred Collection/Volume: RED 10 Volume : 2.0 mL Serum Minimum Volume: .6mL s Alternative Collection: REDSS, Green Na Hep

TAT: Report available: 3 Days Test Schedule: Monday-Friday Morning Method: Tandem Mass Spectometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Methylmalonic Acid Quantitative Clinical Data: Test is useful in the determination of certain genetic disorders and Vitamin B12 deficiency.

Specimen Stability: Room Temperature: 4 days Refrigerated: 7 days TC# 34879

Processing: Centrifuge the specimen as soon as possible after complete clot formation has taken place. Transfer the serum into a Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: Random Urine in AP99 Container Volume: 1.7 mL Urine Do not use preservatives.

TAT: Report available: 4 Days Test Schedule: Two days a week; p.m. Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Methylmalonic Acid Clinical Data: Methylmalonic Acid (MMA) is useful to diagnose and monitor vitamin B12 Urine (cobalamin) deficiency, and to diagnose and monitor patients with methylmalonic acidemia. Specimen Stability: Room Temperature: Unacceptable Refrigerated (cold packs): 12 days Frozen -20 degrees C: 30 days Frozen -70 degrees C: 90 days Do not use preservatives. Test Code: SJC TC 16508X

Processing: Rejection Criteria: Samples stored at room temperature or acidified urine. Transport: Tranport on ice.

Preferred Collection/Volume: 24 hr. Urine (No preservative) Volume: 4.0mL u Absolute Minimum Volume: 1.0mL u

TAT: 24 Hours Microalbumin Level 24 Test Schedule: Daily Hour Urine Method: Turbidometry Test Facility: AWL

Refrigerate during collection. Preservatives are not acceptable. Test is for quant. Microalbumin and Albumin Excretion Rate. For Microalbumin Screen order panel UMS

Processing: Record collection interval hrs. Measure and record total vol. Aliquot to 12x75 tube.

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Refrigerate

Preferred Collection/Volume: AP99 Volume: 4.0mL u Absolute Minimum Volume: 1.0mL u

TAT: 24 Hours Test Schedule: Daily Microalbumin Level Method: See Individual Assays Urine Test Facility: AWL

Panel for Microalbumin Screen. See panel UMQ for Quant. Microalbumin. Collect without additives

Processing: Aliquot to 12x75 tube. Do not freeze. Refrigerate

Preferred Collection/Volume: Formalin Fixed Paraffin Embedded Tissue Specimen Volume: Approximately 1 cm(2) of tumor and normal tissue are required. This can be 1 cm(2) in aggregate (eg, 5 unstained slides each with 0.2 cm(2) of tumor and normal tissue).

TAT: 10 days Test Schedule: Paraffin-embedded tissue already mounted on slides received by Tuesday will be set up on Thursday. Paraffin-embedded tissue already mounted on slides received Friday will be set up the following Tuesday. To allow time for slide preparation if a paraffin block is sent, blocks must be received by Monday for Thursday setup and by Thursday for Tuesday setup. Method: A polymerase chain reaction (PCR)-based assay is used to test for tumor microsatellite instability with the use of 5 mononucleotide repeats. Test Facility: Mayo Medical Laboratories 3050 Superior Drive NW Rochester, MN 55901

Clinical Data: Lynch syndrome, is an autosomal dominant inherited cancer syndrome that predisposes individuals to the development of colorectal, endometrial, gastric, upper urinary tract, and other cancers. Individuals with HNPCC/Lynch syndrome have a germline mutation in 1 of several genes involved in DNA mismatch repair (MMR). The majority of mutations associated with HNPCC/Lynch syndrome occur in MLH1 and MSH2; however mutations in MSH6 and PMS2 have also been identified. There are now several strategies for evaluating individuals whose personal and/or family history of cancer is suggestive of HNPCC/Lynch syndrome. Tumors from individuals with HNPCC/Lynch Microsatellite syndrome demonstrate microsatellite instability (MSI), characterized by numerous alterations in a Instability HNPCC type of repetitive DNA called microsatellites. Two distinct MSI tumor phenotypes have been described: MSI-H (instability in >30% of microsatellites examined) and MSS/MSI-L (instability in <30% of microsatellites examined). The MSI-H phenotype is associated with germline defects in either the MLH1, MSH2, MSH6, or PMS2 gene, and is the primary phenotype observed in tumors from patients with HNPCC/Lynch syndrome. Immunohistochemistry (IHC) is a complementary testing strategy to MSI testing. Most MSI-H tumors show a loss of protein expression for at least 1 of the 4 MMR genes described above. Loss of expression of protein(s) within the tumor is helpful in identifying which corresponding gene(s) to target for mutation analysis. Although MSI and IHC are best interpreted together, they are also available separately to accommodate clinical situations in which there are barriers to performing these tests concurrently (eg, financial concerns, specimen requirements). Testing is typically first performed on the tumor of an affected individual and in the context of other risk factors, such as young age at diagnosis or a strong family history of colon cancer or other HNPCC/Lynch syndrome-related cancers. If defective DNA mismatch repair is identified within the tumor, mutation analysis of the involved can be performed to identify the causative germline mutation and allow for predictive testing of at risk individuals. Of note, MSI-H phenotypes and loss of protein expression by IHC have also been demonstrated in various sporadic cancers, including those of the colon, endometrium, and stomach. Absence of MLH1 and PMS2 protein expression within a tumor, for instance, is most often associated with a somatic alteration in individuals with an older age of onset of cancer than typical HNPCC/Lynch syndrome families. Therefore, an MSI-H phenotype or loss of protein expression by IHC within a tumor does not distinguish between somatic and germline mutations. Genetic testing of the gene indicated by IHC analysis can help to distinguish between these two possibilities. In addition, when absence of MLH1/PMS2 are observed, MLBRF/87931 MLH1 Hypermethylation and BRAF Mutation Analyses, Tumor or MLH1H/87978 MLH1 Hypermethylation Analysis, Tumor may also help to distinguish between a sporadic and germline etiology.

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It should be noted that MSI testing is not a genetic test, but rather helps to stratify the risk of having an inherited cancer predisposition syndrome, and identifies patients who might benefit from subsequent genetic testing. Immunohistochemistry is available as an add-on to this test (IHCO/29004 Mismatch Repair (MMR) Protein Immunohistochemistry Only, Tumor). If both MSI and IHC are desired, please order the profile test, HNPCC screen (HNPCC/17073 Hereditary Nonpolyposis [HNPCC] Screen). See Hereditary Nonpolyposis Colorectal Cancer Testing Algorithm in Special Instructions for additional information. Evaluation for microsatellite instability may also be valuable for clinical decision making. Colon cancers that demonstrate defective DNA mismatch repair (MSI-H) have a significantly better prognosis compared to those with intact mismatch repair (MSS/MSI-L). Additionally, current data indicate that stage II and stage III patients with colon cancers characterized by the presence of defective MMR (MSI-H) may not benefit from treatment with 5-FU alone or in combination with leucovorin (LV). These findings are most likely to impact the management of patients with stage II disease.

Specimen Stability: Specimen Type: Varies Room Temperature:preferred Refrigerated Frozen Test Code: MAYO TC 88566

Processing: Collection Instructions: Submit formalin-fixed, paraffin-embedded tissue block(s) with corresponding hematoxylin-and-eosin (H&E) slides (preferred) or 1 slide stained with H&E and 10 unstained, nonbaked slides (5 slides with 5-micron thick sections and 5 slides with 10-micron thick sections) of the tumor/normal tissue. Forms: Molecular Genetics-Inherited Cancer Syndromes Patient Information Sheet (Supply T519) in Special Instructions

Preferred Collection/Volume: Stool in 10% formalin vial (Pink Para collection kit) Volume 5.0 mL stool Alternative Collection: Duodenal aspirate in 10% formalin vial (Pink Para collection kit) Volume 5.0 mL stool

TAT: Report available: 3-4 Days Test Schedule: 7 days a week; p.m. Method: Light Microscopy, Modified Trichrome Stain Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Microsporidia Stool Clinical Data: Microsporidia infection was first recognized as a cause of chronic diarrhea in patients infected with HIV. Microsporidia may also cause pneumonia, acute bilateral keratoconjunctivitis, and infection of the biliary and pancreatic ducts.

Specimen Stability: Room Temperature: 30 days Refrigerated: 30 days Frozen: Unacceptable Test Code: SJC TC 3562X

Processing: Instructions: 5.0 mL of stool/duodenal aspirate fixed in 10% Formalin. Indicate source of sample in tracking.

Preferred Collection/Volume: Contact Client Services (35 -6900) for collection and handling instructions. The following information will need to be in Order Notes: 1. Test Code, 2. Testing Lab 3. Specimen type 4. Handling instructions.

Miscellaneous Lab Test TAT: Varies Test Schedule: Varies Method: Varies Test Facility: Varies

Patient Preparation: Miscellaneous Laboratory Tests are subject to NWP lab utilization review.

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Please contact Contact Client Services (35 -6900) for complete order information. You will be contacted via staff message if this test cannot be processed as ordered.

Specimen Stability: Varies Test Code: Varies

Processing: Varies Transport: Varies

Preferred Collection/Volume: 1.5 mL citrated plasma. Tube must be filled to at least minimum volume fill line. For neonates at least 2 "mini-collect" tubes are required to achieve sufficient plasma volume

TAT: 1 to 2 days Test Schedule: Daily Method: Clot based Test Facility: AWL

Mixing Study APTT Preparation: If using a butterfly, a citrate discard tube must be used first, when citrate is the first tube collected

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Centrifuge in refrigerated centrifuge. Aliquot carefully using plastic pipette. DO NOT USE FILTERS. Avoid buffy coat. Platelet poor plasma required. Freeze solid Transport: Transport frozen on ice, do not allow to thaw.

Preferred Collection/Volume: 1.5 mL citrated plasma. Tube must be filled to at least minimum volume fill line. For neonates at least 2 "mini-collect" tubes are required to achieve sufficient plasma volume

TAT: 1 to 2 days Test Schedule: Daily Method: Clot based Test Facility: AWL

Mixing Study PT Preparation: If using a butterfly, a citrate discard tube must be used first, when citrate is the first tube collected.

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Centrifuge in refrigerated centrifuge. Aliquot carefully using plastic pipette. DO NOT USE FILTERS . Avoid buffy coat. Platelet poor plasma required. Freeze solid Transport: Transport on ice, do not allow to thaw.

Preferred Collection/Volume: RED SST Volume: 1.0 mL s Alternative Collection: RED 10 LAV EDTA Absolute Minimum Volume: 0.5 mL

TAT: 24 Hours

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Test Schedule: Daily Method: Latex Agglutination Test Facility: AWL

Mononucleosis Screen Mono test is only offered Routine

Processing: Centrifuge, Aliquot RED 10 or LAV EDTA. Note if serum or plasma aliquot on label. Refrigerate if held

Preferred Collection/Volume: Nasal Swab sumitted in Red Capped-LQ Stuart media from each nostril **Samples submitted in Amies media are unacceptable and cause for rejection.

TAT: STAT: Reported within 2 hours of receipt. Test Schedule: Suday through Saturday Method: Real Time PCR Test Facility: Sunnyside and Westside Medical Centers

Clinical Data: Test indicated for ICU and Surgical Patients as indicated by source. This test is always ordered as STAT. MRSA Screen DNA PCR Collection Intstructions: Swab 1-2cm of each nostril with both swabs for three seconds Surgical staff will submit samples for the following sources within two days of surgery: Total Hip Total Kness Spinal Procedures with Hardware CABG Vascular Procedures with Implants

Specimen stable for 24 hours at room temperature and 5 days at 2-8 C.

Processing: Transport immediately to testing location Transport: Transport immediately to testing location Swabs are stable for 24 hours at room temperature, 5 days at 2-8 C.

Preferred Collection/Volume: Liquid Amies Transport Swab (E-swab). Alternative Collection: n/a

TAT: 1 - 2 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: Used for screening for carriage of Methicillin-Resistant Staphylococcus aureus only. Susceptibilities will not be performed. MRSA Screening Patient History: n/a Culture Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired collection/transport device Greater than 48 hours post collection Dry Swab

Processing: Hold at room temperature Transport: Room temperature

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Preferred Collection/Volume: RED 10 NO Gel Barrier Tubes Volume: 2.0 mL s Absolute Minimum Volume: 1.0 mL s

TAT: 48 Hours Test Schedule: Mon, Wed, Fri Method: Immunoassay MSAFP4 Screen Test Facility: AWL

Requisition from provider may accompany specimen, or be sent electronically via EPIC. If paper requisition is presented, attach extra aliquot label to it and send with the specimen. Dispatch specimen and labeled requisition together. If MAP is ordered from EPIC, discard extra aliquot label

Processing: Centrifuge, aliquot, refrigerate

Preferred Collection/Volume: Liquid Amies Transport Swab (E-swab). Alternative Collection: n/a

TAT: 2 - 3 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: Used for screening for carriage of Methicillin-Resistant Staphylococcus aureus and Methicillin-susceptible Staphylococcus aureus. Susceptibilities will not be performed. MSSA/MRSA Screening Patient History: n/a Culture Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired collection/transport device Greater than 48 hours post collection Dry Swab

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: RED 10 and CSF Volume: 2.0 mL Serum and 5.0 mL CSF

TAT: Report availabe: 4 Days Test Schedule: Monday-Friday, a.m. Method: Mylein Basic Protein:Immunoassay IGG SYNTHESIS RATE:Nephelometry Oligoclonal Bands, CSF:Isoelectric Focusing Protein Electrophoresis, Serum and CSF:Electrophoresis, Spectrophotometry Test Facility: Quest Diagnostics Nichols Institute Multiple Sclerosis 14225 Newbrook Drive Profile Chantilly, VA 20153

Patient Preparation: Includes: Myelin Basic Protein, IgG Synthesis Rate, Oligoclonal Bands (CSF), Eletrophoresis, Protein and Total Protein (CSF), Protein Electrophoresis, Serum

Specimen Stability: Room temperature: Not Established Refrigerated: Serum: 7 days, CSF:Not Established Frozen: Not Established Test Code: CHANTILLY TC 11186

Processing:

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Serum : Centrifuge and aliquot into Referred Tests aliquot tube. CSF: Do not centrifuge Transport: Transport Serum and CSF on ice together

Preferred Collection/Volume: RED SST Volume: 1.0 mL s

TAT: Report available: Next Day Test Schedule: Mon-Fri Morning Method: Immunoassay Test Facility: Aiport Way Regional Laboratory Sam/Tox Department

Clinical Data: Mumps is an acute, usually self-limited systemic illness characterized by parotidites, high fever and fatigue. One third of infections are asymptomatic. A live attenuated Mumps Antibody IgG vaccine is available. The mumps EIA IgG antibody test is sufficiently sensitive to establish immune status of the patient.

Specimen Stability: Room temperature: 4 days Refrigerated: 7 days Frozen : 30 days This test is used for immunity screening. To order mumps for case/contact diagnosis see Mumps Antibody IgM

Processing: Centrifuge Transportation: Tranpsort to AWL in blue tote

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next day Test Schedule: Set up: Tuesday, Thursday morning Method: Enzyme Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Mumps Antibody IgM Chantilly, VA 20153 Specimen Stability: Room Temperature: 4 Days Refrigerated: 7 Days Frozen: 30 Days CHANTILLY TC 53142

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: Preferred Specimen: CSF, Sterile leak-proof container Volume 3.0 mL Oral/Buccal Swab in VCM Volume: 3.0 mL -or- Saliva in VCM Volume: 3.0 mL -or- Throat Swab in VCM Volume: 3.0 mL -or- Urine, first morning in AP99 Volume: 50 mL Alternative Collection: 3 mL Nasopharyngeal Swab VCM; Refrigerated; Stability:

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__Room Temperature: Unacceptable __Refrigerated: 4 days __Frozen: 30 days (-70c) -OR- 3 mL Nasal wash/aspirate (Min1 mL) VCM; Refrigerated; Stability: __Room Temperature: Unacceotable __Refrigerated: 4 days __Frozen: 30 days (-70c) -OR- 3 mL CSF (Min1 mL) VCM; Refrigerated; Stability: __Room Temperature: Unacceptable __Refrigerated: 4 days __Frozen: 30 days (-70c)

TAT: Report available: 14 Days Test Schedule: Monday-Sunday Morning Method: Culture Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: A n identification is performed on all inoculated cellular monolayers at 14 days at no additional charge. Mumps Virus Culture Specimen Stability: CSF __Room Temperature: Unacceptable __Refrigerated: 72 hours __Frozen: Unacceptable Oral/Buccal Swab __Room Temperature: Unacceptable __Refrigerated: 4 days __Frozen: 30 days (-70c) Saliva __Room Temperature: Unacceptable __Refrigerated: 4 days __Frozen: 30 days (-70c) Throat Swab __Room Temperature: Unacceptable __Refrigerated: 4 days __Frozen: 30 days (-70c) Urine, first morning __Room Temperature: Unacceptable __Refrigerated: 72 hours __Frozen: Unacceptable Test Code: CHANTILLY TC 2062

Processing: Body fluids to be submitted in VCM Transport tube must be mixed with an equal amount of the VCM Transport Medium. Do not place small volumes of fluid directly into the tube as this causes over-dilution of the specimen. M4 Transport Medium (refrigerated stability: 72 hours) is also acceptable. Transport: Transport on ice

Preferred Collection/Volume: Preferred Specimen: MUSCLE: If a specific muscle is not dictated by the clinical presentation, the following muscles are preferred.In order of preference: Quadriceps, Biceps, Deltoid Adequate size: 1cm x 0.5cm x 0.5cm Needle biopsies are acceptable but not recommended. Avoid end stage muscle. Take biopsy away for a tendon insertion site. No infiltration with local anesthetic.

TAT: Results generally available 14 days after receipt of specimen. Test Schedule: Mon-Fri, 8am-5pm

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Test Facility: OHSU Laboratories Surgical Pathology 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Clinical Data: GENERAL: Correct interpretation of muscle and nerve biopsies is strongly dependent on the clinical setting. It is therefore imperative that pertinent clinical information, including medications and the name of the physician responsible for the patient, accompanies the specimen. This may be either faxed (503-494-2025) the day the specimen is shipped, or perferably e-mailed to [email protected]. E-mail information may be sent in advance as long as the approximate date of surgery is indicated. Specimens without history will be evaluated by H&E stain only.

Send to OHSU ASAP Wrap specimen loosely in saline-moistened gauze or Telfa. Do not immerse in saline. Place wrapped specimen into dry container e.g. specimen cup, and seal. Surround container with wet ice. Do not ship on dry ice. Pack in a Styrofoam container. If the specimen cannot be delivered to OHSU by 4:00pm, the following procedure can be followed: Muscle Biopsy The specimen should be retained at the sending lab until the following OHSU workday. Specimens should be kept on saline-moistened (not soaked) gauze, in a dry closed container, in the refrigerator. If the following day is a Saturday or a holiday, then the specimen should be held (even over the weekend if necessary), until the next available OHSU workday. The next morning, OHSU should be contacted prior to shipping, and then the specimen shipped in the morning on moistened gauze, in a dry container, on wet ice, to Lab Central at OHSU. This is not the recommended treatment for optimal preservation of the specimen. Consideration should be given to rescheduling the biopsy to allow coordination between the sending facility and OHSU. In all cases, it is imperative to notify the Neuromuscle Lab, 503 494-6781, 8:00am to 4:30pm before sending the specimen. Test Code: OHSU TC 121-8033

Processing: PREPARATION FOR SHIPMENT: Wrap specimen loosely in saline-moistened gauze or Telfa. Do not immerse in saline. Place wrapped specimen into dry container e.g. specimen cup, and seal. Transport: Wrap muscle biopsy in sterile gauze. Place in sterile container with enough sterile saline to dampen gauze (use as little saline as possible. Transport container submerged in wet ice.

Preferred Collection/Volume: RED10 Volume: 2mL s

TAT: 7-21 days Test Schedule: Test Method: RIA Immunoprecipitation Test Location: Athena Diagnostics , Inc. Four Biotech Park 377 Plantation Street Worcester, MA 01605 Phone: 1 (800) 394-4493 Musk Quant Titers AB Test Detects antibodies to AChR receptor in patients with MG, negative results reflex to MuSK Quantitative Titers Antibody Test, #482 Type of Disorder: Neuromuscular Disorders Typical Presentation: Limb weakness, ptosis, diplopia, dysarthria, dysphagia Disease(s) tested for: Myasthenia Gravis (MG)

Specimen Stability : Send refrigerated; Do not freeze. TC# 483

Specimen Processing:

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Centrifuge and aliquot into Referred Tests aliquot tube. Transportation: Transport to Airport Way Regional Laboratory in blue tote

Preferred Collection/Volume: CSF or BODY FLUID: 1.0 mL in sterile container. SPUTUM, BRONCH LAVAGE or WASH: 5.0 10.0 mL in sterile container. TISSUE: Place tissue in 0.5 mL sterile saline for transport.

TAT: 1-4 days Test Schedule: Batched 2-4 times per week Method: Real-Time Polymerase Chain Reaction (PCR) Test Facility: Providence Laboratory Services

Clinical Data: To perform this test it is essential to know if the submitted specimen has been processed (digestion and decontamination procedure). This test should not be used as a substitute for culture. It should be used as an adjunct to culture. A negative result does not rule out disease. Notes: This is an amplified method used to detect Mycobacterium tuberculosis complex nucleic acid Mycobacterium in the raw specimen. It is used as an aid in the rapid diagnosis and treatment of a possible Tuberculosis DNA PCR tuberculosis infection. A negative result does not rule out the presence of PCR inhibitors in the patient specimen or MTB DNA concentrations below the level of detection of the assay. Performance characteristics were determined by PHS Infectious Diseases and Molecular Diagnostics laboratory.

Specimen Stability: Room Temperature: 1 Hour Refrigerated: 7 Days Frozen: 1 Month Rejection Criteria: Leaking specimens Test Code: PROVIDENCE TC 44656

Processing: Place tissue in 0.5 mL sterile saline for transport. Transport: Transport on ice.

Preferred Collection/Volume: RED10 NO Gel Barrier Tubes Volume: 1mL s/p Absolute Minimum: 0 . 5mL s/p Alternate Collection: Grn Na Hep or LAV EDTA

TAT: 2 days Test Schedule: Monday through Saturday Test Method: Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Mycophenolic Acid Level Clinical Significance Mycophenolic acid is an immunosuppressant used in tissue transplants. It prevents graft rejection by the host's immune system. It is very important to monitor its level. Too little of this drug will cause graft rejectoin, while too much will lead to infection. Monitoring its level is essential to optimize therapeutic effects, avoid toxicity, and assure compliance.

Transport Temperature Refrigerated (cold packs) Specimen Stability Room temperature: 72 hours Refrigerated: 14 days Frozen: 60 days

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Reject Criteria Received room temperature • Improperly labeled • Left at room temperature >72 hours • Specimens collected in gel barrier tubes • Specimens collected in other tube types not specified TC# 10662

Processing: Centrifuge and aliquot serum/plasma samples into Referred Tests aliquot tube. Transportation: Transport to AWL in blue tote.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Saturday Morning Method: Enzyme Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Mycoplasma Antibody Clinical Data: Enzyme Immunoassay Mycoplasma are the smallest of the free-living IgG organisms. M. pneumoniae causes approximately 10-20% of all cases of pneumonia. These pneumonias that can affect otherwise healthy individuals, are commonly referred to as walking and atypical pneumonias.

Specimen Stability: Room Temperature: 4 days Refrigerated: 1 week Frozen: 1 month Test Code: CHANTILLY TC 21892

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Friday Morning Method: Enzyme Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Mycoplasma Antibody Clinical Data: Mycoplasma are the smallest of the free-living organisms. M. pneumoniae IgM causes approximately 10-20% of all cases of pneumonia. These pneumonias that can affect otherwise healthy individuals are commonly referred to as walking and atypical pneumonias.

Specimen Stability: Room Temperature: 4 days Refrigerated: 1 week Frozen: 1 month Test Code: CHANTILLY TC 47882

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

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Preferred Collection/Volume: Preferred Specimen: Culture, Ureaplasma urealyticum: Preferred Specimens: Urogenital (vaginal, cervical, urethral swabs or secretions); Respiratory (sputum, bronchial washing, trancheobronchial secretions, bronchoalveolar lavage, nasopharyngeal or throat swabs), sterile body fluids, tissue and wounds. Swab of urogenital specimen, sterile fluid, tissue, or respiratory samples, submitted in VCM Transport Medium, refrigerated (cold packs) Mycoplasma hominis Culture: Respiratory samples -or- Sterile Fluid -or- Tissue -or- swab Urogenital Specimen Instructions: Urogenital (vaginal, cervical, urethral swabs or secretions);Respiratory (sputum, bronchial washing, trancheobronchial secretions, bronchoalveolar lavage, nasopharyngeal or throat swabs), sterile body fluids, tissue, and wounds. Alternative Collection: Culture, Ureaplasma urealyticum: Random urine, semen, transtracheal aspirate (neonates), or CSF (neonates) Urine pellet, submitted in VCM (or M4) Transport Medium, refrigerated (cold packs) --or-- Swab of urogenital specimen, sterile fluid, tissue, or respiratory samples, submitted in M4 transport medium, refrigerated (cold packs) Mycoplasma hominis Culture: pellet Urine VCM; Refrigerated; -OR- Swab Urogenital Specimen M4 Transport Media; Refrigerated; -OR- pellet Urine M4 Transport Media; Refrigerated; Mycoplasma or -OR- Ureaplasma Species 1 mL Sterile Fluid M4 Transport Media; Refrigerated; Culture -OR- Tissue M4 Transport Media; Refrigerated; -OR- 1 mL Respiratory samples M4 Transport Media; Refrigerated;

TAT: Report available: 10 Days Test Schedule: Monday-Sunday Morning, Afternoon, Night Method: Culture Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Culture, Ureaplasma urealyticum: Ureaplasma urealyticum and Mycoplasma hominis are primarily associated with genital tract colonization and disease in adults and respiratory tract colonization and disease in newborns. Though controversial, these organisms have been associated with endometritis, chorioamnionitis, premature rupture of membranes, stillbirth, premature birth, low birth weight, postpartum infections, and infertility. Of particular concern is the causual relationship between central nervous system infections in the premature newborn and U. urealyticum. The serious complications of disease associated with Ureaplasma and Mycoplasma infections have led to an increased demand for laboratory identification of these organisms. The extremely small amount of genetic material possessed by these organisms makes them demanding in their nutritional requirements for cultivation. Mycoplasma hominis Culture: Ureaplasma urealyticum and Mycoplasma hominis are primarily associated with genital tract colonization and disease in adults and respiratory tract colonization and disease in newborns. Though controversial, these organisms have been associated with endometritis, chorioamnionitis, premature rupture of membranes, stillbirth, premature birth, low birth weight, postpartum infections, and infertility. Of particular concern is the causal relationship between central nervous system infections in the premature newborn and U. urealyticum. The serious complications of disease associated with Ureaplasma and Mycoplasma infections have led to an increase demand for

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laboratory identification of these organisms. The extremely small amount of genetic material possessed by these organisms makes them demanding in their nutritional requirements for cultivation.

Specimen Stability: Room temperature: Unacceptable Refrigerated: 48 hours Frozen: 30 days ( -70ºC ) Test Code: CHANTILLY TC 871X

Processing: Culture, Ureaplasma urealyticum Specimen Collection/Timing Swab: Collect specimen using a calcium-alginate swab, dacron, or polyester-tipped swab on a plastic or wire shaft. Place swab in a VCM Transport Tube and refrigerate. Tissue: Submit tissue specimen in a sterile cup with sufficient VCM Transport Medium to prevent drying, or place tissue in a VCM Transport Tube and refrigerate. Fluid: Submit fluid, including urine, in a sterile, leakproof container, or add 1-3 mL of specimen directly into a VCM Transport Tube and refrigerate. Urine: Centrifuge at 3000 rpm for 15 minutes. Suspend sediment in VCM Transport. Use a 1:1 volume of urine in VCM Transport if the specimen is not centrifuged. M4 Transport Medium is also acceptable. Transport: Transport on ice

Preferred Collection/Volume: Bronchial lavage/wash in Sterile Container, leak-proof Volume:1.0 mL -OR- Sputum in Sterile Container, leak-proof Container Volume:1.0 mL -OR- Nasopharyngeal Swab Volume:3.0 mL VCM; Refrigerated; -OR- Nasopharyngeal Swab Volume:3.0 mL M4-Multi use media, viral transport; -OR- Throat Swab Volume:3.0 mL VCM; -OR- Throat Swab (Min0.3 mL) Volume:3.0 mL Mycoplasma M4-Multi use media, viral transport; pneumoniae by PCR TAT: Report available: 2 Days Test Schedule: Monday-Sunday; p.m. Method: Real-Time PCR Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave, Suite 200 Cypress, CA 90630

Specimen Stability: Room Temperature: 48 hours Refrigerated: 14 days Frozen: 30 days Test Code: SJC TC 15498X TO FOCUS TC 46300

Processing: 1 mL bronchial lavage/wash or sputum (Mimimum: 0.3 mL) in a sterile, screw- capped container or 3 mL throat swab or nasopharyngeal swab (Minimum: 0.3 mL) in Multi- Microbe Transport Medium (M4) or VCM (green-cap) tube or equivalent (UTM). Respiratory Samples in M4 or VCM Transport Medium: Use sterile vials containing 3 mL of sterile M4 or VCM Transport Medium. If using swabs, use only sterile dacron or rayon swabs. Do NOT

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use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator sticks off near the tip to permit tightening of the cap. Sputum: Collect in a sputum collection kit or a sterile, plastic, container with a leakproof cap. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Report available: 5 Days Test Schedule: Two Days a week Method: Western Blot Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: MAG, Western Blot with Reflexes, is useful in detecting antibodies associated Myelin Associated with autoimmune peripheral neuropathy. Glycoprotein Antibody Patient Preparation: Overnight fasting is preferred.

Specimen Stability: Room Temperature: 24 Hours Refrigerated: 7 Days Frozen: 30 dDys If the MAG Ab IgM, WB, result is positive, then both MAG-SGPG Ab (IgM), EIA, and MAG Ab (IgM), EIA, will be performed at an additional charge. Test Code: SJC TC 10063

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: CSF in Sterile Container, leak-proof Volume: 1.0 mL

TAT: Report available: Next Day Test Schedule: Monday-Friday Evening Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Myelin Basic Protein Clinical Data: The concentration of MBP is often increased in patients with demyelinating CSF diseases, such as multiple sclerosis and may be increased in patients with head injury, CNS trauma, tumor, stroke, and viral encephalitis.

Specimen Stability: Room Temperature: 7 days Refrigerated: 14 days Frozen: 1 Year Test Code: CHANTILLY TC 663

Processing: Do not centrifuge. Transport: Refrigerate if held

Preferred Collection: Random Urine in AP99 or UR VAC with no preservatives Volume: 5 mL

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TAT: 1 day Testing Schedule: Daily Testing Method: UA dipstick RBC/hgb Note: If Myoglobin screen is positive, a quantitative Myoglobin analysis will be reflexed. Freeze specimen immediately and send to Referred Tests department at Airport way Lab. The quantitative test is sent to Legacy for testing. Myoglobin Urine Note: Clean catch is not necessary if Myoglobin Urine is the only test ordered.

Processing: Refrigerate if specimen is not tested within 1 hour. Specimen must be tested within 24 hours of collection. Transportation: Transport specimen refrigerated to testing location.

Preferred Collection : Random Urine Note: Timed urine specimens are acceptable . Volume: 5 mL Minimum Volume: 1 mL

TAT: Report available within 24 hours from when the specimen is received at the testing location. Specimen Stability: Room Temperature: Unacceptable Refrigerated: 24 hours Myoglobin Urine Quant Frozen: 1 month Testing Location: Legacy Lab Central 1225 NE Second Ave. Portland, OR 97232 TC# U MYOGLQ http://www.legacyhealth.org/for-health-professionals/refer-a-patient/laboratory- services/test-table.aspx

Processing : Remove any particulate matter by centrifugation. Transfer urine to a tightly stoppered tube. Freeze specimen as soon as possible. Transportation: Transport frozen specimen on ice, in a thermos to Airport way Referred Test department.

Preferred Collection/Volume: ACD A or B Volume: 5 mL wb Alternate Collection: LAV EDTA For pre-natal or neo-natal specimens, please call the lab at 503-494-5400

TAT: 14-21 days Test Schedule: Weekly

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Test Facility: OHSU Laboratories Molecular Diagnostics Center 2525 SW 3rd Avenue, Suite 350 Portland, OR 97239

Clinical Data: Testing is diagnostic for normal/disease states with > 99% accuracy. Myotonic Myotonic Dystrophy dystrophy 1 is the most common form of inherited adult-onset muscular dystrophy. Affected females DNA Test are at risk for offspring with congenital myotonic dystrophy, characterized by severe hypotonia at birth and learning disabilities or mental retardation. Genetic counseling is indicated for individuals with a family history of DM. Please call the laboratory at 503-494-5400 for more information.

Processing: Do not Centrifuge. Transport: Send Room temperature.

Preferred Collection/Volume: Nasal/Nares: Liquid Amies Transport Swab (E-Swab) Alternative Collection: n/a

TAT: 2 - 3 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: n/a Patient History: n/a Nasal Culture Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired collection/transport device Greater than 48 hours post collection Dry Swab

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection: GRN Micro Li Hep w/sep. Minimum: One full tube (filled to line closest to cap). Preferred: Two tubes Alternative Collection: Amber Micro w/sep Volume: 0.6mL plasma (Absolute minimum volume: 0.25mL plasma)

TAT: STAT 1 Hour Routine 24 Hours Test Schedule: Daily Neonatal Hepatic Method: Photometric, Diazonium Salt (DPD) in acid Function Panel Testing Location: Sunnyside Medical Center and Westside Medical Center

Intended for neonates and infants, generally used up to 30 days of age but continue to order if bilirubin is monitored beyond 30 days of age. Note: TEST NOT PERFORMED AT AWL

Processing: Centrifuge. Protect specimen collected in GRN Li Hep from light. Refrigerate.

Preferred Collection/Volume:

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Mother: LAV EDTA 20cc Blue Cit 10cc Red Top 10cc Father: LAV EDTA 20cc Blue Cit none Red Top none

TAT: 1 Day for Serological Results; 5 Days for additional results Test Schedule: Monday morning through Friday morning. Samples received after 10:00a.m. on Friday or over the weekend will have to be re-drawn. Method: Platelet antibody crossmatch testing of maternal plasma with paternal platelets by Flow cytometry, Platelet antigen typing of both parents' platelets by DNA (PCR) and HLA antibody identification Class I, PAK-12G or MAIPA (if warranted). Test Facility: Puget Sound Blood Center Attn: Platelet Immunology Lab, Rm 619 921 Terry Avenue Seattle, WA 98104

Clinical Data: Thrombocytopenia in the newborn occurs when maternal platelet antibodies cross the placenta, bind to fetal platelets, and the antibody-coated platelets are rapidly cleared from fetal circulation. Sensitization occurs when an incompatible platelet antigen is inherited from the father Neonatal and a maternal antibody is formed in response to this antigen. Unlike erythroblastosis fetalis, NATP can occur in the first-born. NATP is rare, occurring in 1 in 2,000-5,000 births. Most NATP cases are Thrombocytopenia due to the platelet specific antigen PIAl (HPA-1a), but other platelet specific antigens have also Panel been implicated in this disorder. Positive crossmatch results between the maternal plasma and paternal platelets, and platelet antigen discrepancies between paternal and maternal platelets are indicative of NATP. In some cases, a discrepancy between the maternal and paternal platelet antigens cannot be identified but the test results (crossmatch results between the mother's plasma and the father's platelets) are unequivocally positive. This may indicate the presence of a new platelet antigen and is an area of current research. Patient History: In addition to the sample requirements, the Platelet Immunology Supervisor must be contacted to discuss the patient's clinical history. The supervisor can be reached at 206- 292-6542

Patient Preparation: Requires collection of mother and father (no specimens are obtained from the infant). Order should be under MOTHER'S HRN. Collect Mon-Wed AM ONLY. Specimens must reach AWL by 14:00 same day. Labeling: If a specimen is identified by name, there must also be a numeric identifier which may include Hospital number, SS#, birth date, or other coded identifier. A draw date should be on the sample but the sample will still be accepted if the draw information is on the RFT

Transport same day. Test Code: PBSC TC 3670-00

Processing: Do not centrifuge. CORE Lab: Deliver to Referred Tests immediately.

Preferred Specimen: Red SS

Alternative: Red Minimum Volume: 0.5 mL

TAT: Negative 2 days Positve 3 days Testing Schedule: Monday -Friday;8am Specimen Stability: Room Temperature: 72 hours Refrigerated: 28 days Frozen: 28 days Rejection Criteria: Gross hemolysis, gross Icterus

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Testing Location: Mayo Medical Laboratories: 3050 Superior Drive NW Rochester, MN 55901 Test Code:NMOCS http://www.mayomedicallaboratories.com/test-catalog/ Neuromyelitis Optica IgG Include relevant clinical information, name, phone number, mailing address, and email address (if applicable) of ordering physician.

Processing: Centrifuge specimen (if not collected in a Red SS, aliquot specimen). Transportation: Send specimen in tube rack, in Blue tote and send to Airport Way Referred Test department

Preferred Collection/Volume: NSE is high in platelets and RBC. Plasma and hemolyzed specimens are not acceptable. RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available:2-3 Days Test Schedule: Two Days a Week; Morning Method: Enzyme Immunoassay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: NSE is useful in monitoring disease progression and therapy in patients with Neuron Specific Enolase small cell and neuroendocrine tumors, such as , medullary thyroid carcinoma and pheochromocytoma, and other malignancies such as pancreatic islet cell carcinoma.

Specimen Stability: Room Temperature: 24 hours Refrigerated: 3 days Frozen: 1 month NSE is high in platelets and RBC. Plasma and hemolyzed specimens are not acceptable. Test Code: SJC TC 34476X

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 1 mL Serum

TAT: 6 days Test Schedule: Tues, Thurs, Sun night Method: Indirect Immunofluorescence Assay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: This test was developed and its performance characteristics have been determined by Quest Diagnostics. It has not been ceared or approved by the U.S. Food and Drug Administration. The FDA has determined that such cearance or approval is not necessary.

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Performance characteristics refer to the analytical performance of the test. If the Hu Ab, IFA, Serum, result is positive, then Hu Ab, Western Blot, Serum, will be performed at an additional charge (CPT: 84181). If the Hu Ab, Western Blot, Serum, result is positive, then Hu Ab Titer, Serum, will be performed at an additional charge (CPT: 86256).

Patient Preparation: A fasting sample is required

Neuronal Nuclear Type Note: Test performed at Quest San Juan via Quest Chantilly 2 Antibody Specimen Stability: Room temperature: 7 days Refrigerated: 14 days Frozen: 21 days Test Code: Quest TC 37053

Processing: Centrifuge and aliquot serum into a referred tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: Verify info and complete missing information on NBS form is complete and accurate. Fill circles completely. Newborn screening kit

TAT: Test Schedule: Method: Test Facility: Oregon State Lab Newborn Screen 3150 NW 229th Ave., Suite 100 Oregon Hillsboro, OR 97124-6536

Specimen stability: Room Temp

Processing: Allow sample to air dry completely (2-4 hours) in a horizontal position with spots exposed. Transport room temp. Do not place n plastic bag for transport Transport: Send Room Temperature

Preferred Collection/Volume: Dark Blue EDTA or Serum Volume:: 1 mL Serum or Plasma

TAT: 1 day Test Schedule: Thursday Method: Inductively Coupled Plasma/Mass Spectrometry (ICP/MS) Test Facility: NMS LABS 3701 Welsh Road Willow Grove, PA 19090

Clinical Data: Test is for overexposure/poisoning determination and evaluation of orthopedic Nickel implants

NOTE: Test performed at NMS via Quest Chantilly. Specimen Stability: Room Temperature: 14 day(s) Refrigerated: 14 day(s) Frozen (-20 °C): 14 day(s) Test Code: Quest TC 6620

Processing: Centrifuge and aliquot into trace metal free aliquot tubes Transport: Refrigerate if held.

Preferred Collection/Volume: 24 Urine in Acid Washed Container (Without Preservative) Volume 7.0 mL urine

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TAT: Report available: Next day Test Schedule: Monday-Friday Afternoon Method: Inductively Coupled Plasma/Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Patient Preparation: Patient should refrain from taking mineral supplements at least three days prior to sample collection (Refer Patient to Clinician to determine if safe to refrain from dietary supplements) Nickel 24 Hour Urine Specimen Stability: Room temperature: 48 hours Refrigerated: 30 days Frozen: 30 days Test Code: CHANTILLY TC 14521

Processing: Do not use powdered gloves when aliquoting specimen. Mix sample well and aliquot into acid washed container. Note total volume on sample . Transport: Refrigerate if held

Preferred Collection/Volume: Random Urine Collection Volume: 1mL

TAT: 4 days Test Schedule: Monday, Wednesday, Friday Evening Method: Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: This assay is used for the detection of nicotine and cotinine in urine to determine Nicotine Urine the tobacco exposure status of the individual. Nicotine has a short half-life of approximately forty Qualitative minutes; Its presence may indicate recent tobacco exposure. Continine, the major nicotine metabolite, has a half-life of 24 hours and is detectable for several days after cessation of tobacco exposure.

Specimen Stability: Room temperature: 72 hours Refrigerated: 14 days Frozen: 30 days Test Code: Quest TC 90646

Transport: Refrigerate if held.

Preferred Collection/Volume: Sterile leakproof container/1 mL Alternative Collection: n/a

TAT: 5 - 10 Days Test Schedule: Daily Method: Culture Test Facility: AWL Nocardia Species Screen Culture Clinical Data: Must be included with routine bacterial culture Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Greater than 24 hours post collection

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Processing: Sputum: Refrigerate Tissue or Pleural: Room Temperature Transport: Sputum: Refrigerate Tissue or Pleural: Room Temperature

Preferred Collection/Volume: Harmony Prenatal Test Kit Tubes Volume: 18-20 mL wb **Patient must present with clinician completed requisition. Test cannot be performed without requisition.

TAT: 10 days Test Schedule: Daily Test Method: Cell Free DNA Fragment Analysis Testing Location: Ariosa Diagnostics 5945 Optical Court San Jose, California 95138

Test performed anytime after 10 weeks gestation. Please complete testing requisition. Patient must present completed requisition at time of collection. The Harmony Prenatal Test detects >99% of fetal trisomy 21 cases at a false positive rate of <0.1%

This test does not assess risk for mosaicism, partial trisomies or translocations

The Harmony test is available for all singleton and twin pregnancies, including those conceived by IVF NIPT testing is appropriate for the following women: Non Invasive Prenatal Trisomy (NIPT) { >35 years old { Positive 1st trimester screen { Robertsonian translocation in parent { Previous pregnancy with aneuploidy

Referred Tests: Place Ariosa barcode labels from Arisa test requisition over NW Pathnet barcode labels on specimen tubes. Transport same day or next day Room Temp.

Processing: Keep specimens at room temperature. Do not centrifuge or refrigerate samples. Place samples into white sleeve and into the biohazard bag provided in kit after building samples to transfer list. Place the packaged samples into the aluminum sleeve in between the unfrozen ice packs and into the original box with the requisition. **At no time should the samples be refrigerated or placed on ice. They must remain at room temperature. Transportation: Transport packaged samples in kit collection box with requisition to Airport Way Regional Laboratory Core Laboratory Staff: Please deliver to Referred Tests upon receipt

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 3.0 mL Serum

TAT: Report available: 2 Days Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Nortriptyline is a tricyclic antidepressant used in the treatment of depressive

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disorders. The drugs are specifically useful in treatment of autonomous or endogenous depressions. Side effects are primarily anticholinergic consisting of dry mouth, epigastric distress, constipation, palpitations, blurred vision, urinary retention, tachycardia, and hypotension. Toxicity can lead to arrhythmias, coma and death.3 mL serum collected in a red-top tube.

Patient Preparation: Collect just before next dose or at least 12 hours after last dose.

Specimen Stability: Nortriptyline Level Room Temperature: 5 Days Refrigerated: 7 Days Frozen: 30 Days Test Code: CHANTILLY TC 272.

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: Random Urine in AP99 Volume: 2.0 mL -or- 2 Hour Urine Collection Volume: 2.0 mL

TAT: Report available: Next Day Test Schedule: Monday-Saturday Evening Method: Enhanced Chemiluminescence Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

N-Telopeptide Cross Clinical Data: NTx is useful to assess bone resorption in patients with metabolic bone disease. Links Urine The test is also useful in monitoring therapy to slow or halt osteoporotic bone loss. A decline of 30% or more of NTx over a six month period suggests effective therapy.

Specimen Stability: Room Temperature: 72 Hours Refrigerated: 5 Days Frozen: 1 Month Test Code: CHANTILLY TC 14466

Processing: Acidified specimen is not acceptable. Do not use preservatives. Random Urine: Discard the first morning void. Collect the second morning void. Mix well. 2 Hour Urine: Discard the first morning void. Collect the void during a 2-hour period afterward. Mix well. Transport: Refrigerate if held

Preferred Collection: GRN Li Hep PST Alternative Collection: RED SS LAV EDTA Volume: 0.5mL plasma or serum (Absolute minimum volume: 0.2mL plasma or serum

NTpro-BNP TAT: Stat: 1 Hour Routine: 24 Hours Test Schedule: Daily Test Method: Immunoassay Testing Location: Sunnyside Medical Center and Westside Medical Center Add On Testing Stability: 3 days if refrigerated @ 2-8 degrees celsius

Processing: Centrifuge, refrigerate.

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Preferred Collection/Volume: Gastroccult card inocculated by clinian or gastric contents in clean container.

TAT: 1 day Test Schedule: Daily Method: Gastroccult Occult Blood Gastric Test Facility: SMC, WMC Fluid Preparation: Clinician collected.

pH is not tested on this method in the Lab

Processing: Apply a small amount of gastric material to reaction area of card.

Preferred Collection/Volume: Small amount of patient collected stool in special container with preservative. Alternative Collection: N/A

TAT: 3 days from receipt Test Schedule: M-F Method: Immunochemical Occult Blood Stool Test Facility: AWL only Immunoassay Specimen stable 15 days once collected at room temperature and 30 days when refrigerated.

Processing: Patient mails specimen in pre addressed mailer, or may drop off at lab sites. Transport: Forward to AWL if mailer dropped off at lab site.

Preferred Collection/Volume: Small amount of patient collected stool in special container with preservative Alternative Collection: n/a

TAT: 3 days from receipt Test Schedule: M-F Method: Immunochemical Occult Blood Stool Test Facility: AWL only Outreach Immunoassay Preparation: Patients receive kit via mail with instructions and mailer. This test is for Outreach program use only. Labeling: N/A

Processing: Patient mails specimen in pre addressed mailer, or may drop off at lab sites. Transport: Forward to AWL if mailer dropped off at lab site.

Preferred Collection/Volume: AP99 Random Urine Volume: 50 mL u Absolute Minimum Volume: 10 mL u

TAT: 24 Hours Opiate Screen Urine Test Schedule: Daily Method: Immunoassay Test Facility: AWL

Included in drug screen panels DSA, DSB and DSC

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Processing: Refrigerate if held overnight

Preferred Collection/Volume: AP99 Random Urine Volume: 50 mL u Absolute Minimum Volume: 10 mL u

TAT: 24 Hours Test Schedule: Daily Opiates/Oxys Urine Method: GC/MS Confirm Test Facility: AWL

To order a drug screen see panels OPI, DSA, DSB, or DSC. This panel is reflexed to confirm codeine, morphine, hydrocodone, hydromorphone, oxycodone and/or oxymorphone

Processing: Refrigerate if held overnight

Preferred Collection/Volume: Green NA HEP Volume: 1-3 mL Plasma Alternative Collection: Serum or LAV EDTA Volume: 1-3 mL

Test Facility: OHSU Laboratories Biochemical Genetics 2525 SW 3rd Avenue, Suite 350 Portland, OR 97239

Organic Acid Plasma Clinical Data: Not recommended except for confirmation of a few specific disorders. URINE IS Screen PREFERRED SAMPLE

Specimen Stability: Room Temperature: One hour Frozen (-20 °C): 2 month(s) Test Code: OHSU TC 067-6330

Processing: Centrifuge within one hour and aliquot.Note if serum or plasma on specimen. Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: 3-6 mL aliquot of urine, preferably obtained during acidosis or after overnight fast Pediatric SpecimenRequirements: 3 mL urine

Method: Gas Chromatography / Mass Spectrometry Test Facility: OHSU Laboratories Biochemical Genetics 2525 SW 3rd Avenue, Suite 350 Portland, OR 97239 Organic Acids Screen Clinical Data: Indication: Performed on acutely ill neonates, or any patients with suspected or Urine proven metabolic acidosis. Other indications include severe infections, intermittent coma, Reye syndrome, vomiting, failure to thrive, hypotonia, lethargy, hypoglycemia, athetosis, ataxia, myopathy or peculiar smell; also convulsions or cerebral palsy if combined with any of the above. May also be indicated if a positive test for ketones or ketoacids is obtained during amino acid / metabolic screen. Patient History: MUST PROVIDE CLINICAL INDICATION AND COMPLETE LIST OF MEDICATIONS, ANTIBIOTICS, VITAMINS, SPECIFIC DIET OR FORMULA, ETC

Patient Preparation: If patient has intermittent symptoms, sample should be obtained when patient is symptomatic.

Specimen Stability: Frozen only

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Test Code: OHSU TC 067-6331

Processing: Freeze immediately. Transport frozen on ice. Transport: Transport frozen on ice.

Preferred Collection/Volume: AP99 Random Urine Volume: 50 mL u Absolute Minimum Volume: 10 mL u

TAT: 24 Hours Test Schedule: Mon - Sat Organic Base Drug Method: GC/MS Screen Test Facility: AWL

Test includes OTC medications and prescription drugs

Processing: Refrigerate if held overnight

Preferred Collection/Volume: Organism submitted on media plate or slant Alternative Collection: n/a

TAT: Up to 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: Contact Microbiology Laboratory before submission Organism Identification Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Consult with Microbiology Lab for any submission questions

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection/Volume: Organism submitted on media plate or slant Alternative Collection: n/a

TAT: Up to 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: Contact Microbiology Laboratory before submission Organism Susceptiblity Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Consult with Microbiology Lab for any submission questions

Processing: Hold at room temperature Transport: Room temperature

Preferred Collection: RED SST Alternative Collection: Grn Li Hep PST

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RED 10 Volume: 2.0mL serum/plasma (Absolute Minimum Volume: 0.5mL serum/plasma)

TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Osmolality Method: Freezing Point Test Facility: Sunnyside and Westside Medical Centers Add on Stability: 24 hours from collection

Processing: Centrifuge and Aliquot Transport: Refrigerate if held

Preferred Collection/Volume: Stool Volume 1 mL submitted in plastic Container, leak proof

TAT: Next Day Test Schedule: Monday-Friday Afternoon Method: Freezing Point Depression Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: No age/sex specific ranges for this test. In patients with unexplained diarrhea, osmolalities < 290 mOsm/kg indicate contamination of stool with water or dilute urine or the presence of a gastrocolic fistula and ingestion of hypotonic fluid. Osmolalities > 290 mOsm/kg (up Osmolality Stool to 600 mOsm/kg) are common because of bacterial metabolism of fecal carbohydrate during storage of the stool sample. Even higher values can be observed with ingestion of large amounts of poorly absorbable carbohydrate or dietary fiber, with fecal contamination by concentrated urine, or with gastrocolic fistula and ingestion of hypertonic fluids.

Specimen Stability: Room Temperature: unacceptable Refrigerated: unacceptable Frozen: Not established (-20c) Cause for Rejection: Formed stool will not be accepted. Test Code: Quest TC 968

Processing: Freeze sample as soon as collected. Transport: Transport frozen on ice.

Preferred Collection: Random Urine in AP99 Volume: 2.0mL u (Absolute Minimum Volume: 1.0mL u)

TAT: 24 Hours Test Schedule: Daily Method: Freezing Point Osmolality Urine Test Facility: Sunnyside Medical Center and Westside Medical Center Add on Stability: 24 hours from collection

Processing: Aliquot into 12x75 tube Transport: Refrigerate if held.

Preferred Collection: Stool: Parasite Collection Kit Volume: Fill line on container Alternative Collection:

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Sputum: Sterile leakproof container Volume: 3mL Urine: 24 hour urine container Volume: 24 hour collection

TAT: 1 - 3 Days Test Schedule: Daily Method: Microscopy Testing Location: AWL Rejection Criteria:

{ Expired collection/transport device

{ Stool: Greater than 4 days post collection (preserved specimen)

Ova and Parasites { Stool: Greater than 30 minutes post collection (unpreserved specimen).

{ Sputum: Greater than 24 hours post collection

{ Overfilled collection vial

{ No observable specimen in collection vial

{ S tool containing barium

Patient Preparation: Fresh stool must be added to parasite kit vials within 30min. Clinical Data: If specific parasite is suspected, document for laboratory.

Processing: Hold at room temperature Transport: Room temperature Note: If specimen is a 24hour urine, send entire 24 hour urine collection to AWL Laboraotry

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: 3 days Test Schedule: Set up: Wed evening Method: Immunofluorescence Assay (IFA) Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130 Ovarian Ab Screen w Reflex To Titer Clinical Data: Ovarian Antibody is found in patients with premature ovarian failure, Addison's disease, and polyendocrinopathy syndromes.

Specimen Stability: Room temperature: 7 days Refrigerated: 14 days Frozen: 30 days Test Code: CHANTILLY TC 35062 TO SJC TC 10328N

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: 24 Urine Collection

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Volume 10.0 mL urine Collect urine with 25.0 mL 6N HCl to maintian a pH below 3. May be collected without preservative.

TAT: Report available: Same Day Test Schedule: Mon, Wed, Fri Evening Method: Spectrophotometry (SP) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Diagnostic for hyperoxaluria in stone disease and ileal resection

Patient Preparation: Patient should refrain from taking excessive amounts of Ascorbic Acid or Oxalate 24 Hour Urine Oxalate-rich foods (i.e., spinach, coffee, tea, chocolate, rhubard) for at least 48 hours before the collection period. (Refer Patient to Clinician to determine if safe to refrain from dietary supplements)

Specimen Stability: Room temperature: 6 days Refrigerated: 7 days -20° C: 4 months -70° C: Not established Test Code: CHANTILLY TC 6816

Processing: Mix sample well and aliquot. Note on sample total volume and preservative used during collection. Adjust pH to < 3 with 6N HCL if specimen collected without preservative. Transport: To AWL in blue tote. Refrigerate if held

Preferred Collection/Volume: RED10 **No Gel Barrier* Volume: 1.0mL s Alternative Collection: LAV EDTA Volume: 1.0mL p

TAT: 2 Days Test Schedules: Mon-Sat afternoon Test Method: Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Oxcarbazepine Clinical Significance Metabolite Level Oxcarbazepine (trileptal) is an anti-convulsant used for treating generalized tonic-clonic and partial seizures. It can be administered alone or as an adjunct to other anti-convulsants.

Specimen Stability Room temperature: 72 hours Refrigerated: 14 days Frozen: 60 days TC# 36637

Processing: Centrifuge and aliquot into Referred Tests aliquot tube Transportation: Transport to AWL in blue tote

Preferred Collection/Volume: AP99 Random Urine Volume: 50 mL u Absolute Minimum Volume: 10 mL u

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TAT: 24 Hours Test Schedule: Daily Method: Immunoassay Oxycodone Screen Test Facility: AWL Urine Included in drug screen panels DSA, DSB and DSC. Test for screen of oxycodone and/or oxymorphone

Processing: Refrigerate if held overnight

Preferred Collection: Heparinized Syringe Alternative Collection: Dk Grn Li Hep Absolute Minimum Volume: 0.2 ml wb for testing Note: Tube must be full

TAT: 1 Hour Test Schedule: Daily Method: Co-oximetry Test Facility: Sunnyside Medical Center and Westside Medical Center

Note: Central or mixed venous samples should be collected in a blood gas syringe. Oxygen Status Panel Peripheral venous samples may be collected in a blood gas syringe for testing at (O2Hb, Hgb, SO2, Met- the collection site only or a filled green lithium heparin top tube without gel barrier. Hgb, Co-Hgb). Do not transfer samples from tubes to syringes or vice versa. Keep samples sealed and free from exposure to air. Arterial and capillary spec must be collected at Sunnyside or Westside Medical Centers. This is a component of blood gas. ARTERIAL BLOOD GAS: Not collected by Lab staff

Processing: DO NOT REMOVE CAPS Transport: Deliver to lab immediately. Transport at room temprature and analyze syringes within 30 minutes of collection. If necessary (more than 2 hrs for tube collection) use a cab for delivery.

Preferred Collection/Volume: U ndiluted feces collected into clean, dry container. Submit Do not add fixative or preservative. Specimen should be kept cold until delivered to the laboratory. Volume: 1 gram stool

TAT: 2 Days Test Schedule: Tuesday, Thursday Morning Test Method: Immunoassay (IA) Testing Location: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway Pancreatic Elastase-1 San Juan Capistrano, CA 92690-6130

Clinical Significance The Elastase-1 is a quantitative enzyme linked immunosorbent assay for measuring concentrations of elastase-1 in feces as an aid in diagnosis of the exocrine pancreatic function. Alternative Names(s) Elastase-1, Pancreatic

Specimen Stability

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Room Temperature: 5 days Refrigerated: 1 week Frozen: 1 year Transport Temperature Refrigerated TC# 146938

Processing: Keep sample cold Transportation: To AWL on ice

Preferred Collection/Volume: LAV EDTA Volume: 2.0 mL Plasma

TAT: Report available: 4 Days Test Schedule: Thursday Morning Method: Extraction Radioimmunoassay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Pancreatic polypeptide tests are used in the assessment of pancreatic tumor burden and to assist in the early diagnosis of pancreatic tumors. The measurement of pancreatic Pancreatic Polypeptide polypeptide concentrations is also used to monitor pancreatic carcinoma therapy and to predict the recurrence of pancreatic tumors.

Patient Preparation: Overnight fasting is preferred.

Specimen Stability: Room Temperature: Unacceptable Refrigerated: 1 Week Frozen: 28 Days Test Code: SJC TC 4789X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube and freeze. Transport: Transport frozen on ice.

Preferred Collection/Volume: Cerebral Spinal Fluid Volume: 4 mL csf Absolute Minimum: 3 mL csf

TAT: 8 days Test Schedule: ANNA-1: Monday through Friday; 11:30 a.m. ANNA-2: Monday through Friday; 11:30 a.m. ANNA-3: Monday through Friday; 11:30 a.m. AGNA-1: Monday through Friday; 11:30 am PCA-1: Monday through Friday; 11:30 a.m. PCA-2: Monday through Friday; 11:30 a.m. PCA-Tr: Monday through Friday; 11:30 a.m. Amphiphysin: Monday through Friday; 11:30 a.m. CRMP-5-IgG: Monday through Friday; 11:30 a.m. Paraneoplastic autoantibody Western blot confirmation: Monday through Friday; 8 a.m. NMO-IgG: Monday through Friday, 8 a.m. CRMP-5-IgG Western blot: Monday through Friday; 8 a.m.

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GAD65 antibody assay: Monday through Friday; 6 a.m. Amphiphysin Western blot: Monday through Friday, 8 a.m. Test Method: Indirect Immunofluorescence Assay (IFA), Western Blot, Immunoprecipitation Assay Testing Location: Mayo Medical Laboratories 3050 Superior Drive NW Rochester, MN 55901 Telephone 800-533-1710 507-266-5700 FAX 507-284-4542

Useful For An aid in the diagnosis of paraneoplastic neurological autoimmune disorders related to carcinoma of lung, breast, ovary, thymoma, or Hodgkin's lymphoma. In patients with a history of tobacco use or other lung cancer risk, or if thymoma is suspected, PAVAL/83380 Paraneoplastic Autoantibody Evaluation, Serum is also recommended. Clinical Information Several antineuronal and glial autoantibodies are recognized clinically as markers of a patient's immune response to specific cancers (paraneoplastic autoantibodies). Seropositive patients present with neurologic symptoms and signs in >90% of cases. The cancers are most commonly small-cell lung carcinoma (SCLC), ovarian (or related mullerian) carcinoma, breast carcinoma, thymoma, or Hodgkin's lymphoma. The cancers may be new or recurrent, are usually limited in metastatic volume, and are often occult by standard Paraneoplastic Auto AB imaging procedures. Detection of the informative marker autoantibodies allows early CSF diagnosis and treatment of the cancer, which may lessen neurological morbidity and improve survival. Serum is the preferred specimen for paraneoplastic autoantibodies. However, cerebrospinal fluid (CSF) results are sometimes positive when serum results are negative (especially for CRMP-5 and other inflammatory central nervous system autoimmunity). Additionally, CSF is more readily interpretable because it generally lacks the interfering nonorgan-specific antibodies that are common in serum of patients with cancer. Because neurologists typically perform spinal taps in these patients, we recommend that CSF be submitted with serum, either for simultaneous testing or to be held for testing only if serum is negative. CRMP-5-IgG Western blot is also performed by specific request for more sensitive detection of CRMP-5-IgG. Testing should be requested in cases of subacute basal ganglionic disorders (chorea, Parkinsonism), cranial neuropathies (especially loss of vision, taste, or smell), and myelopathies.

Rejection Criteria: Hemolysis Mild OK; Gross reject Lipemia Mild OK; Gross reject Icterus Mild OK; Gross reject Specimen Stability Information CSF Refrigerated (preferred) 14 days Ambient 72 hours

Transportation : Transport to AWL on ice.

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Collection Instructions Preferred Collection/Volume: RED 10 Volume: 4.0 mL s

TAT: 10 Days Schedule: ANNA-1: Monday through Thursday, Sunday; 10:30 p.m ANNA-2: Monday through Thursday, Sunday; 10:30 p.m. ANNA-3: Monday through Thursday, Sunday; 10:30 p.m. AGNA-1: Monday through Thursday, Sunday; 10:30 p.m. PCA-1: Monday through Thursday, Sunday; 10:30 p.m. PCA-2: Monday through Thursday, Sunday; 10:30 p.m. PCA-Tr: Monday through Thursday, Sunday; 10:30 p.m. Amphiphysin: Monday through Thursday, Sunday; 10:30 p.m. CRMP-5-IgG: Monday through Thursday, Sunday; 10:30 p.m. Striational (striated muscle) antibodies: Monday through Thursday, Sunday; 10:30 p.m. P/Q-type calcium channel antibody: Monday, Wednesday, Friday; 6 a.m. N-type calcium channel antibody: Monday, Wednesday, Friday; 6 a.m.. ACh receptor (muscle) binding antibody: Monday through Thursday; 6 p.m., Saturday; 10 a.m. AChR ganglionic neuronal antibody: Tuesday, Thursday, Sunday; 6:00 a.m Neuronal (V-G) K+ channel autoantibody: Tuesday, Thursday, Sunday ; 6 a.m Paraneoplastic Auto AB Serum Paraneoplastic autoantibody Western blot: Monday, Wednesday, Friday; 6 a.m. CRMP-5-IgG Western blot: Monday through Friday; 6 a.m. NMO-IgG: Monday through Friday; 9 a.m. Amphiphysin Western blot: Tuesday, Thursday; 6 a.m. GAD65 antibody assay: Monday through Thursday, Sunday; 8 a.m. ACh receptor (muscle) modulating antibodies: Monday through Thursday; 11 a.m. Methodology: ANN1S/83381, ANN2S/83382, ANN3S/83137, PCABP/83383, PCAB2/83138, PCATR/83076, AMPHS/83386, CRMS/83077, AGN1S/89080, NMOS/83185: Indirect Immunofluorescence Assay (IFA) STR/8746: Enzyme Immunoassay (EIA) CCPQ/81185, CCN/81184, GD65S/81596, ARBI/8338, ARMO/83378, GANG/84321, VGKC/89165: Radioimmunoassay (RIA) WBN/83108, CRMWS/83107, ABLOT/89381: Western Blot Tested At: Rochester Mayo Laboratories

Test is useful for: Serological evaluation of patients who present with a subacute neurological disorder of undetermined etiology, especially those with known risk factors for cancer Directing a focused search for cancer Investigating neurological symptoms that appear in the course of, or after, cancer therapy, and are not explainable by metastasis Differentiating autoimmune neuropathies from neurotoxic effects of chemotherapy Monitoring the immune response of seropositive patients in the course of cancer therapy Detecting early evidence of cancer recurrence in previously seropositive patients

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Specimen Stability Room Temp: 72 hours Refrigerated: 14 days Frozen: no TC #83380

Specimen Handling Processing: Centrifuge and aliquot. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 2.0 mL cyst fluid Alternative Collection: Any other collection container Absolute Minimum Volume: 1.0 mL cyst fluid

TAT: 4 Days Test Schedule: Mon, Thur Parathyroid Hormone Method: Chemiluminescent Immunometric Assay Cyst Fluid Test Facility: AWL

For Cyst Fluid only

Processing: Freeze in container originally received. DO NOT TRANSFER Transport: Transport on ice. Do not allow specimens to thaw

Preferred Collection/Volume: GRN Na Hep Volume: 0.5 mL Plasma

TAT: Report available: 4-7 Days Test Schedule: Monday-Thursday Morning Method: Immunoassay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Parathyroid Related Clinical Data: Test is used in the differential diagnosis of hypercalcemia and to manage Protein patients with solid tumors and hypercalcemia. Specimen Stability: Room Temperature: 7 days Refrigerated: 7 days Frozen: 28 days Test Code: SJC TC 34478Z

Processing: Centrifuge as soon as possible. Aliquot into Referred Tests aliquot tube. DO NOT submit unspun tubes. Transport: Refrigerate if held

Preferred Collection/Volume: RED 10 Volume: 2.0 mL s

Parietal Cell Antibody TAT: 24 Hours Screen Test Schedule: Mon-Fri Method: Immunofluorescence Test Facility: AWL

Processing: Cent, Aliquot, Refrigerate if held

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Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Report available: 4 Days Test Schedule: Monday-Thursday, Sunday Morning Method: Gas Chromatography Test Facility: National Medical Services 3701 Welsh Rd Willow Grove, PA 19090

Clinical Data: Paroxetine (Paxil) is a psychiatric drug indicated for the treatment of depressive Paroxetine Level illness. Its clinical efficacy is similar to that of the traditional antidepressants, but is reported to produce fewer side effects. Paroxetine's pharmacological effects are thought to be mediated through inhibition of reuptake in the brain.

Specimen Stability: Room Temperature: 7 days Refrigerated: 11 days Frozen: 19 months Test Code: CHANTILLY TC 19559 TO NMS TC 3360B

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: Report available: Next Day Test Schedule: Monday-Saturday Afternoon Method: Enzyme Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Parvovirus B19 Clinical Data: Parvovirus B19 is also known as Fifth Disease, affects primarily children and Antibody IgG causes a rash on the face, trunk, and limbs. Joint pain and swelling is more common in adults. Although one-fifth of those affected have only mild disease, patients with sickle cell anemia or similar types of chronic anemia can suffer from acute anemia. Infection during pregnancy can lead to complications. Antibody IgG persists for years and provides lifetime immunity.

Specimen Stability: Room Temperature: 4 days Refrigerated: 7 days Frozen: 30 days Test Code: CHANTILLY TC 5261

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next day Test Schedule: Monday-Saturday Afternoon Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute

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14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Parvovirus B19 is also known as Fifth Disease, affects primarily children and causes a rash on the face, trunk, and limbs. Joint pain and swelling is more common in adults. Although one-fifth of those affected have only mild disease, patients with sickle cell anemia or similar types of chronic anemia can suffer from acute anemia. Infection during pregnancy can lead to complications. Antibody IgM may be persistent for months after infection and be falsely- elevated. Parvovirus B19 Antibody IgM Specimen Stability: Room temperature: 4 days Refrigerated: 7 days Frozen: 30 days Test Code: CHANTILLYT TC 5260

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: WHITE PPT Volume: 1.0 mL Plasma Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma; Frozen; -OR- Bone Marrow in LAV EDTA Volume 1.0 mL -OR- Amniotic Fluid Volume:1.0 mL Sterile Container, leak-proof; Refrigerated; -OR- 3mm3 Tissue Sterile Container, leak-proof (Fresh); Frozen; NOTE: CSF and synovial fluid are not acceptable sample types.

TAT: Report available: Next Day Test Schedule: Monday-Sunday Morning Method: Real-Time PCR Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Parvovirus DNA PCR Clinical Data: Parvovirus B19 is the cause of an infection known as ''Fifth Disease.'' This infection affects children primarily and causes a rash on the face, trunk, and limbs. Joint pain and swelling is more common in adults. Although a portion of those affected have only mild disease, patients with sickle cell anemia, or similar types of chronic anemia, can suffer from severe acute anemia. Infection during pregnancy can lead to severe complications for the fetus. Infections in immunocompromised patients may lead to serious complications, including nephropathy, myocarditis, and persistent anemia. Although serologic detection of circulating antibodies (Parvovirus B-19 IgM & IgG) is useful to determine recent exposure to the virus, DNA testing provides the most reliable evidence of a persistent infection. Quantitative results of Parvovirus B19 viral load may be useful in determining the response to therapy (usually IVIG) and may be beneficial in assessing the risk for nosocomial transmission.

Specimen Stability: Room Temperature: 4 days Refrigerated: 7 days Frozen: 8 months Test Code: CHANTILLYT TC 5319

Processing: Blood: White PPT: Centrifuge within 2 hours and freeze. LAV EDTA: Centrifuge within 2 hours and aliquot sample into Referred Test aliquot tube. Freeze. Bone Marrow: Collect in sterile tubes containing EDTA as anticoagulant. Store and ship Amniotic fluid:Collect in a sterile, leakproof container and refrigerate for storage and transport.

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Tissue: 3mm3 fresh tissue (sterile, leakproof container) is acceptable. Avoid repeated freezing and thawing of specimens. Transport: Refrigerate if held

Preferred Collection/Volume: Pink EDTA Volume: 5.0 mL Alternative Collection: LAV EDTA RED 10 (do not centrifuge) Absolute Minimum Volume: 1.0 mL Pathologist Report For transfusion reaction workups only. Transfusion Reaction Blood and Blood Component Requisition must be sent with specimens. Attach the extra label marked "FORM" on the requisition in the blank area to the right of the identifier signature. The form label must be dispatched and received just like the specimen. Identification signatures required. Refer to SOP#2.1-18-200 Blood Bank Pretransfusion Orders, Specimen and Patient Identification.

Preferred Collection/Volume: RED 10 Volume: 2.0 mL Serum Alternative Collection: LAV EDTA Volume: 2.0 mL Plasma

Test Schedule: Tuesday Thursday 2nd Shift 3 days Method: High Performance Liquid Chromatography (HPLC) Test Facility: National Medical Services 3701 Welsh Rd Willow Grove, PA 19090 Specimen Stability: Room Temperature: 14 days Refrigerated: 14 day Frozen (-20 °C): 12 months Test Code: NMS TC 3380SP

Processing: Centrifuge and aliquot info Referred Tests aliquot tube.Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 3.0 mL Serum Alternative Collection: LAV EDTA Volume: 2.0 mL Plasma

TAT: 4 days Test Schedule: Thursday Method: Gas Chromatography (GC) Test Facility: National Medical Services 3701 Welsh Rd Pentachlorophenol Willow Grove, PA 19090 Level Patient Preparation: Collect sample at end of shift.

Specimen Stability: Room Temperature: 7 day(s) Refrigerated: 14 day(s) Frozen (-20 °C): 18 month(s) Test Code: NMS TC 3385SP

Processing: Centrifuge and aliquot info Referred Tests aliquot tube.Note if serum or plasma on specimen. Transport: Refrigerate if held.

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Preferred Collection/Volume: LAV EDTA microtainer Alternative Collection: EDTA Microtainer

TAT: 1– 3 working days Test Schedule: Monday – Friday Note: A clinician may request STATpathologist review on weekends or holidays when new leukemia, lymphoma, etc. is suspected. Test Method: Microscopic examination of peripheral blood smear Test Facility: AWL Peripheral Blood Smear A CBC is needed for Peripheral Blood Smear Interpretation. If CBC is not ordered Interpretation same day, contact the ordering clinician for CBC order. Clinical Data: n/a Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: Handle microtainer ASAP and test on-site when possible Transport: Transport to Airport Way Laboratory

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 5.0 mL Serum

TAT: Report available: 4 Days Test Schedule: Monday-Thursday, Sunday Morning Method: Gas Chromatography Test Facility: National Medical Services 3701 Welsh Rd Willow Grove, PA 19090

Clinical Data: Perphenazine is a phenothiazine for treating psychosis. Monitoring its level is Perphenazine Level, GC important to optimize therapy, to avoid toxicity, and to assure compliance.

Specimen Stability: Room Temperature: 7 days Refrigerated: 14 days Frozen: 12 months (-20c) Rejection criterion: The use of (SS, serum separator tube)s is not acceptable. Submission of a serum separator tube will result in cancellation. Test Code: CHANTILLY TC 1989 TO NMS TC 4541SP

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. . Transport: Refrigerate if held

Preferred Collection/Volume: 8 mL Urine

TAT: Thursdays(HPLC): 3 days Friday(HPTLC): 4 days Test Schedule: Thursday, Friday Method: High Performance Liquid Chromatography (HPLC), High Performance Thin Layer Pesticides Screen Chromatography (HPTLC) Test Facility: National Medical Services 3701 Welsh Rd Willow Grove, PA 19090

Clinical Data: Overexposure/Poisoning Determination

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Specimen Stability: Room Temperature: Undetermined Refrigerated: Undetermined Frozen (-20 °C): Undetermined Test Code: NMS TC 3445U

Processing: Freeze sample as soon as collected. Transport: Transport frozen on ice.

Preferred Collection: Heparinized syringe Alternative Collection: GRN Li Hep Sterile Container Volume: 5.0 mL bf (Absolute Minimum Volume: 1.0 mL bf)

TAT: 24 Hours Test Schedule: Daily Method: Potentiometric pH Body Fluid Test Facility: AWL

Note: See panel PPH for thoracentesis and pleural fluids Body Fluid pH performed at AWL, TOX Dept.

Transport: Transport on ice. Do not allow contact with room air. Deliver immediately to lab. DO NOT reject specimen if not collected under these conditions

Preferred Collection/Volume: Capillary tube, heparinized Alternative Collection: Heparinized syringe Volume: 1.0mL (Absolute minimum volume: 0.2mL)

TAT: STAT Test Schedule: Daily pH Fetal Scalp Blood Facility: Sunnyside Medical Center and Westside Medical Center

Note: Specimens for scalp pH are not collected by Lab staff

Processing: Fill tube/capillary completely and test within 10 min. Transport: Transport at room temperature

Preferred Collection: Micro Syringe (heparinized) Alternative Collection: GRN Li Hep 4 Volume: 1.0mL pleural fluid (Absolute Minimum Volume 0.2mL pleural fluid )

TAT: 1 Hour Test Schedule: Daily Method: Potentiometric Test Facility: Sunnyside Medical Center and Westside Medical Center pH Pleural Fluid Note: Used at Medical Centers only. Often in micro syringe sent from floors, or Emerg. Room. Applies to thoracentesis fluid

Transport: Room Temperature if testing within 2 hours, if more than 2 hours transport refrigerated. Do not allow contact with room air. Deliver immediately to Med Center lab. DO NOT reject specimen if not collected under these conditions

Preferred Collection/Volume: 1 mL fresh stool collected in clean container with no preservatives.

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TAT: 1 day Test Schedule: Daily Method: pH paper Test Facility: AWL pH Stool Clinical Data: Patient sent home with instructions and pre-labeled collection container.

Processing: Keep refrigerated. Transport : Transport as soon as possible.

Preferred Collection/Volume: AP99 Random Urine Volume:50 mL u Absolute Minimum Volume: 10 mL u

TAT: 24 Hours Test Schedule: Mon - Sat Phencyclidine Method: GC/MS Confirmatory Test Facility: AWL

Clinical Data: See panel DSC (COMPREHENSIVE URN DRG SCR) or panel OBD (ORGANIC BASE DRUG SCREEN) to order a drug screen for phencyclidine

Processing: Refrigerate if held overnight

Preferred Collection/Volume: GRN Li Hep 4 NO Gel Barrier Volume: 0.5 mL p Alternative Collection: RED 10, LAV EDTA Absolute Minimum Volume : 0.2 mL plasma or serum

TAT: STAT: 1 Hour-Routine: 24 hours Test Schedule: Daily Method: Immunoassay Phenobarbital Level Test Facility: Airport Way Regional Lab

Preferred draw time: Trough - immediately before next dose. Transport STAT requests to Airport Way Regional Lab via City Sprint

Processing: Centrifuge and aliquot. Transportation: Transport to Airport Way Regional lab in blue tote. Refrigerate if held.

Preferred Collection/Volume: Stool in DC99 Volume: 10.0 g Feces Container

TAT: Report available: Next Day Test Schedule: Monday-Friday Afternoon Method: Colorimetric Test Facility: Quest Diagnostics Nichols Institute Phenolphthalein Stool 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Phenolphthalein is a cathartic agent that acts mostly on the colon. Since it takes at least 6 hours to work, it is often taken at bedtime to produce a laxative effect the following morning. 85% of what is ingested is excreted in the feces; the remainder is absorbed and eliminated mostly in the urine. The test is used to detect laxative abuse.

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Specimen Stability: Room Temperature: unacceptable Refrigerated: 1 week Frozen: 1 week (-20c) Rejection criterion: The use of (SS, serum separator tube)s is not acceptable. Submission of a serum separator tube will result in cancellation. Test Code: CHANTILLY TC 466918

Processing: Freeze Transport: Transport frozen on ice

Preferred Collection/Volume: Green NA HEP Volume: 1 mL Plasma

Test Facility: OHSU, Pediatric Metabolic Lab

Patient History: Record clinical indication and meds Specimen Stability: CRITICAL FROZEN

Processing: Centrifuge and aliquot Plasma into a referred tests aliquot tube. Freeze. Transport: Transport frozen on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Report available: Next Day Test Schedule: Monday-Saturday Morning Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Phenytoin Level Free Clinical Data: The unbound (free) drug is the pharmacologically active component of phenytoin. The percent free is influenced by renal disease, pregnancy, protein abnormalities, and other medical conditions.

Specimen Stability: Room Temperature: 5 Days Refrigerated: 7 Days Frozen: 30 Days Test Code: CHANTILLY TC 23692

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: GRN Li Hep 4 No Gel Barrier Volume: 0.5 mL p/s Alternative Collection: RED 10 Absolute Minimum Volume: 0.2 mL p/s

Phenytoin Level Total TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Method: Enzyme Immunoassay Test Facility: AWL, SMC

Preferred draw time: Trough - immediately before next dose

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Processing: Cent. Aliquot. Refrigerate

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p/s Alternative Collection: RED SST or serum acceptable Absolute Minimum Volume: 0.2mL p/s

TAT: STAT: 1 Hour Phosphorus Level Routine: 24 Hours Test Schedule: Daily Method: Photometric, Molybdate Test Facility: AWL, SMC, INT, LVK

Processing: Cent. Refrigerate if held

Preferred Collection/Volume: 24 hr Urine (no preservative) Volume: 4.0mL u Absolute Minimum Volume: 1.0mL u

TAT: 24 Hours Test Schedule: Daily Method: Photometric, Molybdate Phosphorus Level 24 Test Facility: AWL Hour Urine Refrigerate during collection. Preservatives are not acceptable. See panel RPO for random specimen

Processing: Record collection interval hrs. Measure and record total vol. in ARE in LIS. Aliquot to 12x75 tube. Transport: Refrigerate if held.

Preferred Collection/Volume: Random Urine in AP99 Volume: 4.0mL u Absolute Minimum Volume: 1.0mL u

TAT: 24 Hours Test Schedule: Daily Phosphorus Level Urine Method: Photometric, Molybdate Test Facility: AWL

Processing: Aliqout to 12x75 tube. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 1.0-3 mL Plasma Alternative Collection: Serum Volume: 1-3 mL

Test Facility: OHSU Laboratories Biochemical Genetics Phytanic Acid Level 2525 SW 3rd Avenue, Suite 350 Portland, OR 97239

Clinical Data: Indication: For diagnosis of Refsum disease, useful but not definitive for diagnosis of Zellweger syndrome and other peroxisome assembly disorders; very long chair fatty acids, plasma should be ordered to rule out these disorders. If both phytanic and very long chain fatty acids are to be done, one need only draw a single 1-3 mL blood specimen

Specimen Stability:

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Frozen Only Test Code: OHSU TC 067-6335

Processing: Centrifuge within one hour and aliquot. Note if serum or plasma on specimen.Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: SWUBE (PINWORM PADDLE) Alternative Collection: n/a

TAT: 1 day Test Schedule: Daily Method: Microscopy Test Facility: BVT, CPK, DIV, INT, LVK, NLR, ORC, RKW, SAL, SKY, SST, TUA, VAN

Pinworm Exam Clinical Data: Colllection of 3 consecutive daily specimens is recommended. Patient History: n/a

Patient Preparation: Hand out 1 swube per test ordered. Give patient specific written instructions for specimen collection along with each swube. Labeling: n/a

Processing: Room Temperature Transport: Room Temperature

Preferred Specimen/Volume: Vaginal Fluid submitted in AmniSure vial from collection kit. Vial has blue top.

TAT: 1 hour Test Schedule: As needed Test Method: Rapid immunochromatographic Testing Location: SMC, WMC

This test is used to detect the presence of a specific protein found in amniotic fluid. Placental Alpha If present, the patient has likely suffered rupture of fetal membranes and needs Microglobulin-1 Qual immediate care.

Viability of specimen is increased to six hours if refrigerated.

Specimen Processing: Specimen handling is critical! Specimen is viable only 30 minutes post collection. Deliver to testing bench as soon as received. Assure that specimen collection time is accurately reflected in NW PathNet when received. Transport: Collected by clinician and tested at SMC and WMC

TAT: 7 days Test Schedule: Thurs a.m Method: Enzyme-Linked Immunosorbent Assay (ELISA) Placental Lactogen Test Facility: Cambridge Biomed Research Group 1256 Soldiers Field Rd Brighton, MA 02135

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

Clinical Data: Human placental lactogen (HPL; chorionic somatomammotropin) is a 21, 000 kD polypeptide (similar in structure to growth hormone and prolactin) produced during pregnancy by placental trophoblastic cells. The level of HPL in maternal serum is directly related to placental function and fetal well-being. HPL is detected at about 6 weeks after conception and its

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concentration increases gradually to peak levels (without decreases) until about the 34th week where it remains stable for the remainder of the pregnancy. Consistently low levels throughout pregnancy or a sudden drop in serial determinations are an indication of fetal distress. After normal delivery the HPL concentration falls rapidly to an undetectable level. The HPL levels in serum of women with multiple placenta pregnancies generally exceeds that of single placenta pregnancies. This is generally noted from the second trimester to delivery.

Specimen Stability: Placental Lactogen Room temperature: 4 hours Refrigerated: 24 hours Frozen: 30 days Test Code: Quest TC 5232

Processing: Centrifuge and aliquot into a Referred Tests aliquot tube. Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: Blue Cit Volume: 2.0 mL Plamsa

TAT: 1-4 Days Test Schedule: Mon-Fri Method: Chromogenic Assay Test Facility: ARUP Laboratories 500 Chipeta Way Salt Lake City, UT 84108-1221 Plasminogen Specimen Stability: Room Temperature: 4 hours Refrigerated: 4 hours Frozen (at or below -20°C): 1 week Test Code: ARUP TC 30190

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: Blue Cit Volume: 1.0 mL Plasma

TAT: 2-5 days Test Schedule: 3 days a week Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130 Plasminogen Activator Inhibitor Clinical Data: Increased activity is associated with increased risk of arterial thrombosis, such as with unexplained premature myocardial infarction. As an acute phase reactant, the activity is increased after an acute event. Studies suggest PAI-1 may be a prognostic marker in early stage breast cancer.

Test Code: SJC TC 36555X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze Transport: Transport frozen on ice.

Preferred Collection/Volume: RED 10 Volume: 5.0 mL serum NO Gel Barrier

Test Facility: American Red Cross 3131 North Vancouver Avenue Portland, OR 97227

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Patient History: Complete ARC tissue typing form available from Client Services.

SPECIMEN STABILITY: ROOM TEMP 24 HOURS Platelet Antibody American Red Cross-HLA

Processing: Do not centrifuge Transport: Send Room Temperature

Preferred Collection: LAV EDTA Alternative Collection: LAV EDTA Microtainer Minimum Volume: 1 mL

TAT: 24 hours for routine specimens Testing Method: 5-Part diff Sysmex Hematology cell counter Stability:

{ Specimen is stable for 24 hrs at room temperature for all parameters.

{ Refrigerated specimens are stable for 72 hours for hemogram (ABC) parameters. Platelet Count { Specimens 48-72 hrs old require evaluation of all abnormal differentials.

Rejection Criteria:

{ Clotted specimens

{ Hemolyzed specimens

{ IV contaminated specimens

Note : Platelet Count is included in CBC and ABC.

Processing: Handle microtainers ASAP and test on-site when possible. Transport: Transport specimens to Airport Way Laboratory.

Preferred Collection/Volume: 2 Blue Cit Do not use pediatric (1.8mL) tubes. Volume: Full Tube

TAT: 1 day Test Schedule: Daily Method: Closure time Platelet Function Test Test Facility: AWL Preparation: Record if patient has taken aspirin within past 14 days.

LKV, NLR, SKY, WSM send to local hospital lab.

Processing: Specimen must be tested within four hours of collection. Call for stat transport if specimen will not reach AWL within 4 hours of collection. DO NOT REFRIGERATE, ROCK or ROTATE. DO NOT SEND THROUGH PNEUMATIC

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TUBE. Transport: Send to AWL in room temp thermos, LVK and Salem labs send to local hospital lab for testing. All other send to AWL.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 4 days a week Test Schedule: 4 days Method: Immunodiffusion (ID) Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Scleroderma may be localized or diffuse [Progressive Systemic Sclerosis (PSS)] that may involve skin, gastrointestinal tracts, lungs, vascular and cardiac systems, and kidneys. PM-1 (PM-Scl) Antibody is present in approximately one-fourth of patients with the PM-1 Antibody polymyositis/scleroderma overlap syndrome, 8% of patients with polymyositis alone and 2-5% of patients with scleroderma alone. Patients who have PM-1 Antibody have a better prognosis than patients with scleroderma

Specimen Stability: Room temperature: 7 days Refrigerated: 14 days Frozen: 30 days Reject Criteria Serum separator tubes (SST) Test Code: SJC TC 37103

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: BAL in sterile leakproof container/ >=1mL Bronchial Brush in sterile leakproof container with 1mLs sterile salines Sputum: Sterile leak proof container >= 1 mL Pleural fluid: Sterile leak proof container >= 1 mL Alternative Collection: n/a

TAT: 1 - 2 Days Test Schedule: Daily Method: Microscopy Test Facility: AWL

Pneumocystis carinii Clinical Data: n/a Stain, Fluorescent Patient History: n/a

Preparation: n/a Labeling: n/a

Rejection Criteria: >48 hours post collection specimen other than respiratory Bronchial brushings submitted in >1 mL sterile saline

Processing: Refrigerate Transport: Send on Ice

Preferred Collection/Volume: LAV EDTA Volume: 5 mL Whole Blood

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Test Facility: OHSU Laboratories Flow Cytometry/Immunology 3181 SE Sam Jackson Blvd, 9th Floor Portland, OR 97239

Clinical Data: Patients with Paroxysmal Nocturnal Hemoglobinuria (PNH) have a defect in the pig-a gene and lack glycosylphosphatidylinositol (GPI) anchored proteins including CD59 (membrane inhibitor of reactive lysis) which protects cells from complement mediated lysis. This test measures the presence or absence of CD59 by flow cytometry. A consultative report will be PNH CD59 CD66b sent.

Specimen Stability: Room Temperature Test Code: OHSU TC 087-0652

Processing: Do not Centrifuge Transport: Send Room Temperature

Preferred Collection/Volume: Heparinized syringe Volume: 1.0 mLs Absolute Minimum Volume: 0.2 mLs

TAT: STAT Test Schedule: Daily POC Blood Gases Method: Arterial Test Facility: SMC

Arterial punctures are not performed by lab staff. Needle must be removed and syringe capped at bedside

Processing: Test within 10 min. of collection. Do not ice specimen.

Preferred Collection/Volume: Capillary tube, heparinized Volume: 1.0 mL wb Absolute Minimum Volume: 0.2 mL wb

TAT: STAT Test Schedule: Daily POC Blood Gases Method: Cap/mixed Test Facility: SMC

Intended for point-of-care neonatal blood gas testing, performed by Nursery staff at SMC. For lab testing, see panel CBG

Processing: Fill tube completely and test within 10 min. DO NOT ICE

Preferred Collection/Volume: GRN Li Hep No Gel Barrier Tubes Volume1.0 mL wb Absolute Minimum Volume: 0.2 mL wb

POC Blood Gases TAT: STAT Venous Test Schedule: Daily Method: Test Facility: SMC

Processing: Fill completely. DO NOT UNCAP. Test within 10 min. of collection. Do not ice

Preferred Collection/Volume: GRN Li Hep 4 Volume: 1.0 mL wb

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Alternative Collection: Heparinized syringe

TAT: 1 Hour Test Schedule: POC BUN Method: Urease, Potentiometric Test Facility: SMC, INT, SKY, NLR, RKW, SAL, VAN, BVT, CAS, DIV, SST, TUA, ORC

Used for iStat testing in Inpatient and ASC units only

Preferred Collection/Volume: GRN Li Hep Volume: 1.0 mL wb Alternative Collection: Heparinized syringe

TAT: POC Chloride Test Schedule: Method: ISE Potentiometry Test Facility:

Used for iStat testing in Inpatient and ASC units only

Preferred Collection/Volume: Capillary tube, heparinized Volume:1.0 mL cb Absolute Minimum Volume: 0.2 mL cb

TAT: STAT Test Schedule: Daily POC Cord Bld Gas Method: Arterial Test Facility: SMC

Intended for point-of-care neonatal blood gas testing, performed by Labor and Delivery nursing staff at SMC

Processing: Fill tube completely and test within 10 min. DO NOT ICE

Preferred Collection/Volume: Capillary tube, heparinized Volume: 1.0 mL cb Absolute Minimum Volume: 0.2 mL cb

TAT: STAT Test Schedule: Daily POC Cord Bld Gas Method: Ion Selective Electrode Venous Test Facility: SMC

Intended for point-of-care neonatal blood gas testing, performed by Labor and Delivery nursing staff at SMC

Processing: Fill tube completely and test within 10 min. DO NOT ICE

Preferred Collection/Volume: GRN Li Hep 4 Volume: 1.0 mL WB Alternative Collection: Heparinized syringe

TAT: POC Creatinine Test Schedule: Method: Enzymatic, Amperometric Test Facility:

Used for iStat testing in Inpatient and ASC units only

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Preferred Collection/Volume: Whole Blood:fingerstick,capillary,venous Volume: 0.2 mL wb

TAT: Immediate Test Schedule: Daily POC Glucose Method: Glucose Oxidase, Amperometric Test Facility: SMC

Test performed only on POC iSTAT analyzers at KSC. DO NOT use EDTA, fluoride, or citrate samples with the iSTAT

Preferred Collection/Volume: LIGREEN4/ 1.0 mL WB Alternative Collection: Heparinized syringe/

TAT: 1 Hour POC Hematocrit Test Schedule: Daily Method: Conductometric Test Facility: SMC

Used for iStat testing in Inpatient and ASC units only

Preferred Collection/Volume: LIGREEN4/ 1.0 mL WB Alternative Collection: Heparinized syringe/

TAT: STAT POC Hemoglobin Test Schedule: Daily Method: Calculated Test Facility:SMC

Used for iStat testing in Inpatient and ASC units only

Preferred Collection/Volume: LIGREEN4/ 1.0 mL WB Alternative Collection: Heparinized syringe/

TAT: STAT POC Ionized Calcium Test Schedule: Daily Method: Ion Selective Elctrode 1 PH Electrode Test Facility: SMC

Used for iStat testing in Inpatient and ASC units only

Preferred Collection/Volume: LIGREEN4/ 1.0 mL WB Alternative Collection: Heparinized syringe/

TAT: STAT POC Lactate Test Schedule: Daily Method: Amperometric Test Facility: SMC

Used for iStat testing in Inpatient and ASC units only

TAT: STAT Test Schedule: Daily POC PCO2 Method: Direct Potentiometry Test Facility: SMC

Clinical Data: Used for iStat testing in Inpatient and ASC units only.

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Preferred Collection/Volume: LIGREEN4/ 1.0 mL WB Alternative Collection: Heparinized syringe/

TAT: 1 Hour POC Potassium Test Schedule: Daily Method: ISE Potentiometry Test Facility: SMC

Used for iStat testing in Inpatient and ASC units only

Preferred Collection/Volume: LIGREEN4/ 1.0 mL WB Alternative Collection: Heparinized syringe/

TAT: 1 Hour POC Sodium Test Schedule: Daily Method: ISE Potentiometry Test Facility: SMC

Used for iStat testing in Inpatient and ASC units only

Preferred Collection: RED 10 Alternative Collection: SerumAcceptable Volume: 1.0 mL Serum Minimum Volume: 0.5 mL

TAT: 5 Days Testing Location: Poliovirus Antibody Focus Diagnostics, Inc. (Types 1-3) 5785 Corporate Ave, Suite 200 Cypress, CA 90630 TC# 8540N http://www.questdiagnostics.com/testcenter/TestCenterHome.action

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Specimen Stability: Refrigerated: 14 days (preferred) Frozen: 30 days

Preferred Collection/Volume: Collect 24-hours urine without preservative. Refrigerate during and after collection. Protect from light. Preferred Specimen: 24-hour urine jug Volume: 10.0 mL Urine

TAT: 2 days Test Schedule: 5 days a week, P.M Method: Colorimetric Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway

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San Juan Capistrano, CA 92690-6130

Clinical Data: Urinary Porphobilinogen is the first step in the diagnosis of acute intermittent prophyria (AIP). AIP is an autosomal dominant disorder characterized by deficiency of porphobilinogen deaminase. An acute attack usually includes gastrointestinal disturbance and neuropsychiatric disorders.

Patient Preparation: The urine should be refrigerated and protected from light during the collection

Porphobilinogen 24 Specimen Stability: Room temperature: Unacceptable Hour Urine Refrigerated: 7 days Quantitative Frozen: 30 days Reject Criteria: Received room temperature • Not protected from light • pH less than 4.0 24-hour total volume must be provided on the test request form. Wrap tube in aluminum foil or use amber tube to protect from light. Test Code: SJC TC 726

Processing: Mix sample well and aliquot. Note on sample total volume. Wrap tube in aluminum foil or use amber tube to protect from light. Transport: Tranport on ice

Preferred Collection/Volume: Random Urine inAP99 Volume: 10.0 mL urine Insturctions: Collect without preservatives and wrap in foil to protect from light.

TAT: 2 days Test Schedule: 5 days a week, P.M Method: Colorimetric Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130 Porphobilinogen Quantitative Urine Specimen Stability: Room temperature: Unacceptable Refrigerated: 7 Days Frozen: 30 Days Reject Criteria: Received room temperature • Not protected from light Test Code: SJC TC 84889N

Processing: Freeze. Transport: Transport frozen on ice

Preferred Collection/Volume: Random Stool in DC99 Volume: 10.0 g Protect from light.

TAT: Next Day Test Schedule: Tuesday; p.m. Method: Fluorometric Porphyrin Stool Test Facility: Quest Diagnostics Nichols Institute Qualitative 14225 Newbrook Drive Chantilly, VA 20153

Specimen Stability: Room temperature: Unacceptable Refrigerated: Unacceptable Frozen: 14 Days Test Code: CHANTILLY TC 35518.

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Processing: Freeze Transport: Transport frozen on ice

Preferred Collection/Volume: 24-hour urine jug Volume: 2.0 mL Protect from light.

TAT: 4-5 days Test Schedule: 5 days a week p.m Method: High Performance Liquid Chromatography (HPLC) Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Clinical use is to diagnose porphyria cutanea tarda, hereditary coproporphyria, Porphyrins 24 Hour variegate porphyria. Urine Specimen Stability: Room temperature: Unacceptable Refrigerated: 7 days Frozen: 30 days Reject Criteria: Received room temperature • Not protected from light • pH less than 4.0 Test Code: SJC TC 729

Processing: Refrigerate and protected from light during and after collection. Mix sample well and aliquot. Note on sample total volume. Transport: Tranport on ice

Preferred Collection/Volume: GRN NA HEP 10 (Whole Blood) Volume: 7.0 mL Whole Blood

TAT: 3 days (not reported on Saturday or Sunday) Test Schedule: Monday through Friday; 8 a.m Method: Spectrofluorometric Test Facility: Mayo Medical Laboratories 3050 erior Drive NW Rochester, MN 55901

Clinical Data: If specimen cannot arrive within 48 hours, order PEWE/31893 Porphyrins Porphyrins Evaluation, Washed Erythrocytes. Fractionated RBC Patient Preparation: Patient should abstain from alcohol for 24 hours. Include a list of medications the patient is currently taking.

Sepcimen Stability: Refrigerated 72 hours Test Code: MAYO TC 88886

Processing: COLLECT MONDAY-THURSDAY MORNING ONLY. Do Not Centrifuge. Immediately place specimen on wet ice. Send with the next courier. Specimen must arrive at AWL same day as collection. Transport: Tranport on ice

Preferred Collection/Volume: Random Urine in AP99. Volume 2.0 mL random urine, no preservative

TAT: Report available: 4-5 days Test Schedule: 5 days a week p.m Method: High Performance Liquid Chromatography (HPLC) Test Facility: Quest Diagnostics Nichols Institute

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33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Specimen Stability: Room temperature: Unacceptable Refrigerated: 7 days Porphyrins Frozen: 30 days Reject Criteria: Fractionated Urine Received room temperature • Not protected from light • pH less than 4.0 Test Code: SJC TC 36592

Processing: Refrigerate and protected from light during and after collection. Transport: Tranport on ice

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5 mL p/s Alternative Collection: RED SST or serum acceptable Absolute Minimum Volume: 0.2 mL p/s

TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Potassium Level Method: ISE Test Facility: All: SMC, AWL, INT, BVT, CAS, LVK, SKY, NLR, RKW, SAL, VAN, DIV, SST, TUA, ORC

iSTAT Testing Performed by LAB Staff: Whole blood from unspun GNS5 acceptable for testing

Processing: Centrifuge. Refrigerate if held. ADD-ON Testing Stability: 24 hrs

Preferred Collection/Volume: 24 hr Urine (no preservative)/ 4.0mL u Alternative Collection: 12X75 aliquot/ 1.0mL u

TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Potassium Level 24 Method: ISE Hour Urine Test Facility: AWL, SMC

Refrigerate during collection. Preservatives are not acceptable. See panel RUK for random specimen

Processing: Record collection interval hrs. Measure and record total vol. in ARE in LIS . Aliquot to 12x75 tube. Refrigerate

Preferred Collection/Volume: AP99 with 12x75 aliq./ 4.0mL u Alternative Collection: / 1.0mL u

TAT: STAT: 1 Hour Routine: 24 Hours Potassium Level Urine Test Schedule: Daily Method: ISE Test Facility: AWL, SMC

Processing: Aliquot to 12x75 tube. Refrigerate

Preferred Collection: Heparinized syringe Alternative Collection: Dk Grn Li Hep Volume: 3.0 mL whole blood (Absolute Minimum Volume: 0.2 mL whole blood)

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TAT: 1 Hour Test Schedule: Daily Method: ISE Test Facility: Sunnyside Medical Center and Westside Medical Center Potassium Whole Blood Processing: Needle must be removed and syringe capped. Analyze within 30 minutes of collection. Transport: Transport at room temperature

Preferred Collection/Volume: Green 3 Sodium Heparin/ 3cc MINIMUM VOLUME 1CC Alternative Collection: n/a

TAT: Routine: 14-21 days Test Schedule: Mon-Fri Method: In situ hybridization on metaphase cells Test Facility: AWL

Prader Willi-Angelman Clinical Data: If ordered in combination with a chromosome study, only 1 specimen is needed. (15p11-q13,15p11.2) Include clinical indication. Mutation Analysis FISH Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: ** No Gel barrier tubes ** Do not Centrifuge. Hold at Room temperature. Do NOT reject centrifuged specimen; contact department at 503-258-6723 (Tie Line: 35-6723) Transport: Room Temperature.

Preferred Collection/Volume: LAV EDTA Volume: 6.0 mL whole blood Min Volume: 2 mL

Test Facility: OHSU Mail code BTE355 3181 S.W. Sam Jackson Park Road Portland, OR 97239

Prader-Willi DNA Clinical Data: Southern blot analysis of genomic DNA digested with specific restriction enzymes is used to assess imprinting and uniparental disomy of the 15q 11-q13 region<>.Reference Range: Methylation Analysis PWS is associated with the functional loss of inprinted genes located in the paternally derived 15q 11-q13 region. This is most commonly the result of either a paternal deletion of the 15q 11-q13 region (75% of cases) or maternal uniparental disomy for chromosome 15 (25% of cases).

Send Room temperature. Test Code: OHSU TC NA102-7100

Processing: Do not Centrifuge. Transport: Send Room temperature.

Preferred Collection/Volume: GNS5/0.5mL p/s Alternative Collection: SSRED5, RD10/0.2 mL p/s

Prealbumin TAT: 24 hrs Test Schedule: Daily Method: Turbidometry Test Facility: AWL

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Processing: Centrifuge. Aliquot if RD10. Refrigerate if held.

Preferred Collection/Volume: Collect without preservative and refrigerate during collection Urine aliquot from a 24-hour urine container Volume 5 mL

TAT: 4 Days Test Schedule: Tues., a.m. Method: Gas Chromatography/Mass Spectrophotometry (GC/MS) Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130 Pregnanetrio 24 Hr Urine Pediatric Patient Preparation: Refrigerate during collection. (Includes Creatinine Labeling: Please provide on the urine container the total 24-hour urine volume. Ratio) Specimen Stability: Room temperature: 8 hours Refrigerated: 48 hours Frozen: 30 days ship sample frozen on dry ice Test Code: Quest TC 15329

Processing: Mix sample well and aliquot. Note on sample total volume and preservative used during collection. Freeze. Transport: Transport frozen on ice.

Preferred Collection/Volume: 24-hour urine jug Volume: 5.0 mL

TAT: 4 Days Test Schedule: Tuesday Morning Method: Gas Chromatography Mass Spectrometry Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway Pregnanetriol 24 Hour San Juan Capistrano, CA 92690-6130 Urine Specimen Stability: Room Temperature: 8 Hours Refrigerated: 2 Days Frozen: 1 Month Test Code: CHANTILLY TC 15329 TO SJC TC 738X

Processing: Mix sample well and aliquot. Note on sample total volume. Transport: Transport frozen on ice

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Daily Test Schedule: 24 Hours Method: EIA Primidone Level Test Facility: Legacy Metrolab, 1225 NE Second Ave, Portland, OR 97232

Rejection Criteria: Specimens collected in gel tubes LEGACY CS: 503-413-1234. NO TEST CODE

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. <> Cab STAT specimens directly to Legacy Central Lab with completed Legacy Requisition (may be obtained from Client

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Services) Complete Specimen Transport Form and send to AWL in the blue tote.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: Dark Blue Serum Volume:1 mL Serum (Min0.2 mL) -OR- LAV EDTA Volume:1 mL Plasma (Min0.2 mL) -OR- Green Na Hep Volume:1 mL Plasma (Min0.2 mL) -OR- Blue Cit Volume: 1 mL Plasma (Min0.2 mL)

TAT: Next Day Test Schedule: Monday- Saturday Evening Method: Immunoassay Test Facility: STAT: Legacy Metrolab 1225 NE Second Ave Procainamide Level Portland, OR 97232 Routine: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Procainamide is used to treat a variety of atrial and ventricular arrhythmias. Metabolism of procainamide produces an active metabolite, N-Acetylprocainamide (NAPA). The concentration of both procainamide and NAPA levels are monitored to assure adequate therapeutic levels of procainamide are achieved and to avoid toxicity.

Patient Preparation: Collect as a trough just before next dose. Labeling: Label tube as Peak or Trough

Specimen Stability: Room Temperature: 4 days Refrigerated: 7 days Frozen: not established Test Code: QUEST TC 3782

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Volume: 1.0 mLs

TAT: 24 Hours Test Schedule: Mon-Sat Progesterone Level Method: Immunoassay Test Facility: AWL

Processing: Centrifuge and aliquot. Refrigerate if held. Freeze if testing is greater than 48 hours.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 4 Days Test Schedule: Monday Night

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Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Patient Preparation: Overnight fasting is required.

Specimen Stability: Proinsulin Room Temperature: 24 hours Refrigerated: 3 days Frozen: 6 months Test Code: CHANTILLY TC 760X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube Freeze. Transport: Transport frozen on ice

Preferred Collection/Volume: RED 10 Volume: 1.5 mL s Alternative Collection: GRN Li Hep GRN Na Hep Absolute Minimum Volume: 1.0 mL s/p

Prolactin Level TAT: 24 Hours Test Schedule: Mon-Sat Method: Immunoassay Test Facility: AWL

Processing: Centrifuge and aliquot. Refrigerate if held.

Preferred Collection/Volume: AP99 Random Urine/ 50 mL u Alternative Collection: / 10 mL u

TAT: 24 Hours Test Schedule: Mon - Sat Propoxyphene Method: GC/MS Confirmatory Test Facility: AWL

See panel DSC (COMPREHENSIVE URN DRG SCR) or panel OBD (ORGANIC BASE DRUG SCREEN) to order a drug screen for propoxyphene

Processing: Refrigerate if held overnight

Preferred Collection/Volume: Urine Sample submitted in PCA3 Collection Container Kit obtained from Airport Way Laboratory Materials Management Clinician will obtain urine sample after DRE exam and submit to laboratory for testing.

TAT: 48 to 72 hours Test Schedule: Test Method: Testing Location: Bostwick Laboratories, PCA3 Plus. Antigen 3 (PCA3) 4355 Innslake Drive, Glen Allen, VA 23060. Client Services Phone: 877-865-3262.

PCA3 Plus ® detects PCA3, a specific gene that is highly expressed in prostate cancer. No other human tissues express PCA3 PCA3 Plus tests for prostate cancer cells that are shed into the urine. The urine sample is sent to Bostwick Laboratories to be tested for genetic expression of the PCA3 gene. If the sample is positive for PCA3, then the patient has a very high likelihood of having prostatic

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cancer.

Processing: Transport entire collection kit to lab with ice pack

Preferred Collection/Volume: RED 10 Volume: 1.0mL s Alternative Collection: RED SST Absolute Minimum Volume: 0.3mL s

Prostate Specific TAT: 24 Hours Antigen Test Schedule: Mon - Sat Method: Chemiluminescent Immunoassay Test Facility: AWL

Processing: Cent. Aliquot. Refrigerate up to 48 hrs. Freeze if greater than 48 hrs before testing

Preferred Collection/Volume: 24 hr Urine collected with no preservatives. Refirgerate during collection. Volume: 4mL u

TAT: 24 Hours Test Schedule: Daily Method: Colorimetric Protein 24 Hour Urine Test Facility: AWL

Refrigerate during collection. Preservatives are not acceptable. See panel RPO for random specimen

Processing: Mix sample well and aliquot. Record total volume on aliquot and Accession Result Entry in NW PathNet prior to sending to AWL.

Preferred Collection: Grn Li Hep PS Alternative Collection: Drk Grn Li Hep, Red 10 or Red SS Volume: 4.0 mL body fluid Absolute Minimum Volume: 1.0 mL body fluid

TAT: Protein Body Fluid STAT within 1 Hour Routine within 24 Hours Testing Schedule: Daily Method: Colorimetric Testing Location: Airport Way Laboratory, Sunnyside Medical Center, and Westside Medical Center.

Processing: Centrifuge and Refrigerate

Preferred Collection: 2 BLUE CIT Tube must be filled to minimum fill indicator Volume: 1mL plasma Neonates minimum volume plasma 100 µl, if not combined with other tests

TAT: 1 Week Test Schedule: Wed Method: Test Facility: AWL

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Patient Preparation: If using a butterfly needle for collection, a citrate discard tube must be used first, when citrate is first tube collected

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check Protein C Activity Processing: Prepare platelet poor plasma on site. Freeze Solid NOTE: Aliquots may be shared with Antithrobin III Activity and Protein S if ordered If needed STAT, contact testing department (AWL-SAM) to make arrangements for same day testing. Specimen must arrive by noon. Transport: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection/Volume: CSF submitted in sterile container or CSF collection container Volume: 1.0 mL csf Absolute MInimum : 0.5 mL csf

TAT: 1 Hour Test Schedule: Daily Method: Colorimetric Test Facility: Sunnyside and Westside Medical Centers Protein CSF Interstate Medical Office

Handle and process all CSF as STAT

Processing: Use tube 4 for testing unless otherwise indicated by clinician. Refrigerate if held. Transportation: Transport to testing location immediately. Testing located at Sunnyside and Westside Medical Centers and Interstate Medical Office.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL s Alternative Collection: RED SST Absolute Minimum Volume: 0.5 mL s

TAT: 24 Hour Test Schedule: Mon - Fri Protein Electrophoresis Method: Electrophoresis Test Facility: AWL

This panel includes a Total Protein result. Immunofixation electrophoresis performed if indicated. IMS (Immunoglobulins) ordered if M-protein detected.

Processing: Centrifuge and aliquot. Refrigerate if held.

Preferred Collection/Volume: 24 hr urine collected without preservative. Refrigerate during collection. Volume: 100mL u in AP99 and 4mL u aliquot tube

TAT: 24 Hours Protein Electrophoresis Test Schedule: Mon - Fri Urine Method: Electrophoresis Test Facility: AWL

Preservatives are NOT acceptable. For random collections, see Urine Protein Electrophoresis, Random Immunofixation electrophoresis performed if indicated

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Processing: Mix sample well and aliqout two samples for testing. Aliquot sample into AP99 and a second aliquot into plastic aliquot tube. Record total volume on both aliquots and in Accession Result Entry in NW PathNet. Tranport: To AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: Random urine submitted in sterile container Volume: 100mL u Minimum Volume: 25mL u

TAT: Test Schedule: Protein Electrophoresis Test Method: Electrophoresis Urine Random Testing Location: Airport Way Regional Laboratory

Processing: Send two samples for testing: the original AP99 collection container plus a urine aliquot tube. Transportation: Transport to both samples to AWL in blue tote. Refrigerate if held.

Preferred Collection: 2 BLUE CIT Tube must be filled to minimum fill indicator Volume: 1mL plasma Neonates minimum volume plasma 100 µl, if not combined with other tests.

TAT: 2-7 days Test Schedule: Once per week Method: Clot based Test Facility: AWL

Patient Preparation: If using a butterfly needle for collection, a citrate discard Protein S Free tube must be used first, when citrate is the first tube collected.

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Prepare platelet poor plasma on site. Freeze solid. NOTE: Aliquots may be shared with Antithrombin III and Protein C if ordered. If needed STAT, contact testing department (AWL-SAM) to make arrangements for same day testing. Specimen must arrive by noon. Transport: Transport frozen on ice. DO NOT ALLOW TO THAW.

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p/s Alternative Collection: RED SST or serum acceptable Absolute Minimum Volume: 0.2mL p/s

TAT: STAT: 1 Hour Protein Total Routine: 24 Hours Test Schedule: Daily Method: Colorimetric Test Facility: SMC, AWL, INT, LVK

Processing: Cent., Refrigerate if held

Preferred Collection/Volume: AP99 with 12x75 aliq./ 4.0 mL u Alternative Collection: / 1.0 mL u

TAT: 24 Hours

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Test Schedule: Daily Method: Colorimetric Test Facility: BVT, SMC, INT, AWL Protein Urine Processing: Aliquot to 12x75 tube. Refrigerate

Preferred Collection/Volume: At least 2 mLs of random urine in AP99 or UVAC (no preservative).

TAT: 24 hours Test Schedule: Daily Method: UA dipstick Test Facility: AWL, Medical Office labs, SMC, WMC Protein Urine Dipstick Preparation: Provide patient with pre-labeld container. Clean catch not necessary if only test ordered.

Processing: Refrigerate if not tested within 2 hours. Must be tested within 24 hours. Transport: Transport refrigerated to testing facility.

Preferred Collection/Volume: Aliquot of random urine/ 2.0 mL u Alternative Collection: / 1.0 mL u

TAT: 24 Hours Test Schedule: Daily Protein/Creatinine Method: See Individual Assays Ratio Urine Test Facility: BVT, SMC, INT, AWL

Can not use urine with preservatives

Processing: Aliquot and Refrigerate

Preferred Collection/Volume: 5.0 mL whole blood, LV5 (EDTA) Alternative Collection: 2.0 mL Whole blood.

TAT: 2-7 days Test Schedule: Tues Method: Invader Assay Test Facility: AWL: Molecular Diagnostics

Prothrombin Gene Clinical Data: N/A Mutation Factor 2 Patient History: N/A

Preparation: N/A Labeling: N/A

Rejection Criteria: Heparinized or frozen specimen.

Processing: Do not centrifuge. Transport: Transport at 2 - 8 C. Do not freeze.

Preferred Collection: BLUE CIT Tube must be filled to minimum fill indicator Volume: 1mL plasma Neonates minimum volume plasma 100 µl, if not combined with other tests.

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TAT: 24 hours routine, 1 hr stat Test Schedule: Daily Method: Clot based Test Facility: AWL, INT, LVK, NLR, SMC, WMC

Preparation: If using a butterfly, a citrate discard tube must be used first, when citrate is the first tube collected.

This test is intended for patients being monitored for coumadin. Protime w INR Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Stable 24 hours at room temp. DO NOT REFRIGERATE. DO NOT UNCAP. If held overnight at collection site: Prepare platelet poor plasma. Freeze solid. DO NOT USE FILTER to aliquot specimen. Plasma is stable 4 hours in uncapped tube Transport: Transport full tubes at room temperature. If frozen, keep frozen during transport.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 5 Days Test Schedule: Monday-Friday Morning Method: Gas Chromatography Test Facility: National Medical Services 3701 Welsh Rd Willow Grove, PA 19090

Protriptyline Specimen Stability: Room Temperature: 14 days Refrigerated: 14 days Frozen: 12 months (-20c) Rejection criterion: The use of (SS, serum separator tube)s is not acceptable. Submission of an (SS) tube will result in cancellation. Test Code: CHANTILLY TC 14369 TO NMS TC 9436SP

Processing: Centifuge as soon as specimen has clotted. Aliquot into Referred tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: SSRED5/ 0.6 mL s

TAT: 1 Week Test Schedule: Wed Method: Chemiluminescent Immunoassay PSA Free and Total Test Facility: AWL

NOTES: Use this panel when Free PSA is requested. Will analyze Total PSA, Free PSA and % Free PSA. Done at AWL

Processing: Cent and aliquot. Refrigerate up to 48 hrs. Freeze if greater than 48 hours before testing

Preferred Collection/Volume: RED 10 Volume: 1.0mL s Alternative Collection: RED SST

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Absolute Minimum Volume: 0.3mL s

TAT: 24 Hours Test Schedule: Mon - Sat Method: Chemiluminescent Immunoassay PSA Total Test Facility: AWL

Processing: Centrifuge and aliquot. Refrigerate if held. Freeze if testing is greater than 48 hours.

Preferred Collection/Volume: RD10/ 1.0 mL s Alternative Collection: SSRED5/ 0.3 mL s

TAT: 24 Hours Test Schedule: Daily PSA Total Method: Chemiluminescent Immunoassay Ultrasensitive Test Facility: AWL

Detection limit is 0.01 ng/mL

Processing: Cent, aliquot, send on ice. Refrigerate up to 48 hrs, freeze if greater than 48 hrs before testing

Preferred Collection: Blue Cit Tube must be filled to minimum fill indicator Volume: 1mL plasma. Neonates minimum volume plasma 100 µl if not combined with other tests.

TAT: 24 hours routine, 1 hr stat Test Schedule: Daily Method: Clot based Test Facility: AWL, INT, SMC, LVK, NLR, WMC

PT Preparation: If using a butterfly, a citrate discard tube must be used first, when citrate is the first tube collected.

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Stable 24 hours at room temp. DO NOT REFRIGERATE. Do not uncap. If held overnight at collection site: Prepare platelet poor plasma and freeze. DO NOT USE FILTER. Transport: Transport full tubes at room temperature. If frozen, keep frozen during transport. Plasma is stable 4 hours in uncapped tube.

Preferred Collection/Volume: Fingerstick collection by technical staff.

TAT: Immediate Test Schedule: M-F Method: CoaguChek PT Whole Blood Test Facility: BVT, CPK, GTW, INT, LVK, NLR, RKW, SAL, TUA

Preparation: Outpatient Fingerstick INR test performed by technologist at certain locations. Patient will be provided with results upon completion of testing.

INR via vein draw is encouraged when results exceed instrument linearity.

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Collection Instructions Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

PTH Parathyroid Intact Specimen Handling Processing: Centrifuge and aliquot. Freeze Transport: Transport frozen on ice.

Parathyroid Intact

Preferred Collection/Volume: LAV EDTA NO Gel Barrier Tubes Volume: 2.0 mL p Absolute Minimum Volume: 1.0 mL p Alternative Collection: Drk GRN Li Hep

TAT: 1 Hour Test Schedule: Daily Method: Immunoassay Test Facility: Sunnyside Medical Center Westside Medical Center

PTH Intraoperative (4 .PTH Baseline 1 Specimens) .PTH Baseline 2 .PTH 5 Minute Post .PTH 10 Minute Post Test is performed daily. Must be pre-scheduled using lab email address: NW SYS LAB KSMC PIO-KPNW. Please notify laboratory prior to the start surgery. For use at Sunnyside and Westside Medical Centers in the surgical unit only.

This panel for use at Sunnyside and Westside Medical Centers in the surgical unit only.

Processing: Centrifuge for two minutes using StatSpin and test immediately. Aliquot after testing for storage.

Preferred Collection/Volume: LAV EDTA NO Gel Barrier Tubes Volume: 2.0 mL p Absolute Minimum Volume: 1.0 mL p Alternative Collection: Drk GRN Li Hep

TAT: 1 Hour Test Schedule: Daily Method: Immunoassay Test Facility: Sunnyside Medical Center Westside Medical Center

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.PTH Baseline 1 .PTH Baseline 2 .PTH 5 Minute Post .PTH 10 Minute Post Test is performed daily. Must be pre-scheduled using lab email address: NW SYS LAB KSMC PIO-KPNW. Please notify laboratory prior to the start PTH Intraoperative surgery. Additional Specimens For use at Sunnyside and Westside Medical Centers in the surgical unit only

This panel for use at Sunnyside and Westside Medical Centers in the surgical unit only.

Processing: Centrifuge for two minutes using StatSpin and test immediately. Aliquot after testing for storage.

Preferred Collection: LAV EDTA Alternative Collection: DRK GRN Li Hep (NO GELL BARRIER TUBES) VOLUME: 2.0 mL p Minimum Volume: 1.0 mL p

TAT: 1 hour PTH Sample 1 Note: This test is for the Surgical Units at Sunnyside Medical Center and Westside Medical Center use only.

Testing is available daily, and must be pre-scheduled using the laboratory email address below. NW SYS LAB KSMC PIO-KPNW Note: Please notify the laboratory prior to the start of surgery.

Processing: Centrifuge for four minutes using StatSpin and test immediately. Note: Aliquot after testing for storage purposes.

Preferred Collection: LAV EDTA Alternative Collection: DRK GRN Li Hep (NO GELL BARRIER TUBES) VOLUME: 2.0 mL p Minimum Volume:

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1.0 mL p

TAT: 1 hour

Note: This test is for the Surgical Units at Sunnyside Medical Center and Westside Medical Center use only.

PTH Sample 2 Testing is available daily, and must be pre-scheduled using the laboratory email address below. NW SYS LAB KSMC PIO-KPNW Note: Please notify the laboratory prior to the start of surgery.

Processing: Centrifuge for four minutes using StatSpin and test immediately. Note: Aliquot after testing for storage purposes

Preferred Collection: LAV EDTA Alternative Collection: DRK GRN Li Hep (NO GELL BARRIER TUBES) VOLUME: 2.0 mL p Minimum Volume: 1.0 mL p

PTH Sample 3 TAT: 1 hour Note: This test is for the Surgical Units at Sunnyside Medical Center and Westside Medical Center use only.

Testing is available daily, and must be pre-scheduled using the laboratory email address below. NW SYS LAB KSMC PIO-KPNW Note: Please notify the laboratory prior to the start of surgery.

Processing: Centrifuge for four minutes using StatSpin and test immediately. Note: Aliquot after testing for storage purposes

Preferred Collection: Blue Cit Note:Tube must be filled to minimum fill indicator. Volume: 1mL plasma Neonates minimum volume plasma 100 µl, if not combined with other tests.

TAT: 24 hours Testing Schedule: Daily Method: Clot based Testing Location: SMC, INT, AWL

Preparation: If using a butterfly, a citrate discard tube must be used first, when

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citrate is the first tube collected. Inpatient, Dialysis patients may be on unfractionated heparin. Heparin shortens the stability of aPTT, therefore centrifugation must occur within 1 hour and testing must occur within 2 hours of collection. Heparinized plasma is stable 2 hours post collection. Avoid agitation of specimen.

Note: Processing centrifuge must be checked periodically for production of platelet PTT poor plasma. Refer to W.I. 1.8B-19-200.

Processing: Stable 10 hours at room temp. DO NOT REFRIGERATE. If held overnight at collection site: Prepare platelet poor plasma. Freeze Solid. Do Not Use Filter. Plasma stable 4 hours once uncapped. Transport: Transport full tubes at room temperature. If frozen, keep frozen during transport.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 3 Days Test Schedule: Tuesday, Thursday, Sunday Night Method: Immunofluorescence Assay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Purkinje cell cytoplasmic (Yo) antibody is found in approximately half of the patients with paraneoplastic cerebellar degeneration and is associated with ovarian, uterine, and small cell lung carcinomas and Hodgkin's lymphoma. Purkinje Cells Antibody Patient Preparation: Overnight fasting is preferred.

If Purkinje Cell (Yo) Ab Screen is positive, then Purkinje Cell Antibody Titer, IFA, Serum, is performed at an additional charge (CPT: 86256). Specimen Stability: Room Temperature: 7 days Refrigerated: 14 days Frozen: 21 days Test Code: CHANTILLY TC 37360 TO SJC TC 37360

Processing: Centifuge as soon as specimen has clotted. Aliquot into Referred tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: Yellow Top ACD-B 6mL Yellow top (Solution B) Volume: 4 mL Whole Blood

TAT: 3 Days Test Schedule: Monday-Saturday Morning Method: Spectrophotometric Test Facility: Mayo Medical Laboratories Pyruvate Kinase 200 First Street SW Rochester, MN 55905

Specimen Stability: Room Temperature: Unacceptable Refrigerated: 20 Days Frozen: Unacceptable Test Code: CHANTILLY TC 6681 TO MAYO TC 8659

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Processing: Do not centrifuge or aliquot. Do not freeze specimen. Transport: Transport on ice

Preferred Collection/Volume: RED 10 Volume: 1 mL Serum Alternative Collection: LAV EDTA Volume1 mL Plasma Green NA HEP Volume: 1 mL Plasma BLUE CIT Volume:1 mL Plasma

TAT: Next day Test Schedule: Mon-Thurs afternoon Fri, Sat evening; Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute Quinidine Level 14225 Newbrook Drive Chantilly, VA 20153

Patient Preparation: Collect just before next dose.

Specimen Stability: Room temperature: 4 days Refrigerated: 7 days Frozen: Not established Test Code: Quest TC 766

Processing: Centrifuge and aliquot Serum/Plasma into a referred Tests aliquot tube. Note on tube if serum or plasma. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 Volume: 2.0 mL Serum

TAT: 30 Days Test Schedule: Monday, Thursday Morning Method: Rapid Fluorescent Foci Inhibition Test (RFFIT) Test Facility: K-State Rabies Laboratory Manhattan/K-State Innovation Center 2005 Research Park Circle Manhattan, KS 66502

Clinical Data: Results are confidential personal health information. All disclosures must be in accordance with HIPAA. In humans, a result of 0.5 IU/mL or higher is considered acceptable, Rabies Virus Antibody according to the World Health Organization (WHO) guidelines; see WHO and Advisory Committee on Immunization Practices documents for additional guidance. Also, there is more information at www.vet.ksu.edu/rabies (Note: the symbol >/= means greater than or equal to).

NOTE: Test performed at Kansas State University via Quest Chantilly Specimen Stability: Room Temperature: unacceptable Refrigerated: 14 days Frozen: 1 month Please note: Referral lab will not release results unless collection date is provided Test Code: QUEST TC 18232

Transport: Transport on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Next Day

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Test Schedule: Tuesday, Thursday Morning Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Circulating immune complexes (CIC) are present in many individuals with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA), especially with any of the vasculitides complications. Levels of CICs have been reported to show correlation with disease activity in that higher levels are reported during active phases of the disease. Raji Cell Assay Specimen Stability: Room Temperature: Unacceptable Refrigerated: Unacceptable Frozen: 30 Days Rejection Criteria: Room temperature and refrigerated samples, serum separator (SS) tubes. Test Code: CHANTILLY TC 2385

Processing: Centrifuge and aliquot into Referred Tests aliquot tube and freeze. Transport: Transport frozen on ice

Preferred Collection: RED SST and LAV EDTA Volume: 2.0 mL s and 3.0 mL wb

TAT: 1 Hour Test Schedule: Daily Method: Immunoassay Test Facility: Sunnyside and Westside Medical Centers Interstate Medical Office Specimen Stability: RED SST: 72 hours at room temperature, 7 days at 2-8C LAV EDTA: 5 days at 2-8C

Note: Collection intended for source patient testing after body fluid exposure. Rapid HIV Test Collect 1 LAV EDTAand 1 RED SST, keep specimens together

Processing: Centrifuge RED SST. Do not centrifuge LAV EDTA. NLR,SKY,WSM: Cab LAV EDTA to Salem Hosp. Hold RED SST for HIV 1/2 Ab Confirmation, if needed. LVK: Cab LAV EDTA to St. John's Hosp. Hold RED SST for HIV 1/2 Ab Confirmation, if needed. INT: Tests Rapid HIV on site, Hold RED SST for HIV 1/2 Ab Confirmation, if needed. All Other Clinics: Transport both specimens in a ziplock bag and send via City Sprint to SMC, WMC, or INT according to routing on label. SMC, WMC and INT : Receive specimens and process/analyze as STAT. Note: If HIV 1/2 Ab Confirmation needed, cab RED SST to AWL for testing.

Preferred Collection/Volume: Yellow Top ACD (whole blood) Volume:10.0 mL Whole Blood

TAT: 2-10 days (not reported Saturday or Sunday) Test Schedule: Monday through Friday Method: EEEV/21065: Consultative Interpretation G6PD/8368, GPI/9158, PK/8659, GLTI/2635, HEXK/2630, RBCE/23544: Kinetic Spectrophotometry (KS) Test Facility: Mayo Medical Laboratories 3050 Superior Drive NW Rochester, MN 55901 RBC Enzyme

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Evaluation (G6PD, Clinical Data: All enzyme defects, including erythrocyte enzyme errors, are inherited; some are Pyruvate Kinase, sex-linked and located on the X chromosome. Some family members have no hematologic Hexokinase, Glucose abnormalities, while others have a hemolytic anemia. For a number of red blood cell enzyme defects (e.g., deficiencies of hexokinase, glucose phosphate isomerase, pyruvate kinase), the sole Phosphate Isomerase) clinical manifestation is hemolytic anemia. Glucose-6-phosphate dehydrogenase deficiency is the most common metabolic error of the red cell and presents with acute hemolytic anemia in response to oxidant stress (e.g., drugs, acute infections, fava bean ingestion). This is a consultative evaluation looking at red cell enzyme defects as the cause for early red cell destruction.

Specimen Stability: Refrigerated: 8 days Test Code: MAYO TC 84161

Processing: Do not centrifuge or aliquot. Do not freeze specimen. Transport: Transport on ice

Preferred Collection/Volume: LAV EDTA **Wrap in foil immediately after collection **Draw samples Sunday through Thursdays only Volume: 1.0 m: Whole Blood.

TAT: Next Day Test Schedule: Monday-Friday Evening Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 RBC Folate Clinical Data: Samples collected Sunday through Thursdays only as testing is time sensitive.

Specimen Stability: Room Temperature: 72 Hours Refrigerated: 72 Hours TC# 14178

Processing: Do Not Centrifuge. W rap sample in foil to protect from light. Transport: Transport to Airport Way Regional Laboratory in blue tote.

Preferred Collection/Volume: COLLECT SUNDAY-WEDNESDAY MORNING ONLY! Preferred Specimen: GREEN NA HEP 6 (Whole Blood) No Gel Barrier Tubes Volume: 5.0 mL Whole Blood

TAT: 3 Days Test Schedule: Mon-Sun All Shifts Method: Spectrophotometric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Red Cell Osmotic Chantilly, VA 20153 Fragility Incubated Clinical Data: Osmotic (RBC) Fragility is used to assess disorders of the erythrocyte membrane. Increased osmotic fragility is found in hereditary spherocytosis, other RBC membrane disorders, and in idiopathic acquired hemolytic anemias. Diminished fragility is seen in conditions in which target cells are found.

Specimen Stability: Room temperature: 48 hours Refrigerated: 72 hours Frozen: Unacceptable Reject Criteria: Hemolysis; Received PST; Received frozen; Received clotted

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Test Code: CHANTILLY TC 139731

Processing: Do not centrifuge or aliquot sample. Sample must be sterile. Clotted and/or hemolyzed samples are unacceptable. Place specimen in a bag labeled "Attn Referred Tests STAT". Call Client Services to alert them to the specimen. Core Lab: Take specimen to Referred Tests immediately. Samples MUST be received in the referral lab Monday through Thursday and ASAP after collection. Transport: Refrigerate if held.

Preferred Collection/Volume: 1 mL of fresh stool collected in a clean container with no preservative. Refrigerate until testing. Alternative Collection: n/a

TAT: 24 hours Test Schedule: Daily Method: Clinitest Reducing Substance Test Facility: AWL Stool Patient Preparation: Patient sent home with instructions and pre-labeled collection container.

Processing: Refrigerate upon receipt. Testing must occur within 24 hours. Transport: Send to AWL as soon as possible.

Preferred Collection/Volume: Random urine AP99/ 2.0 mL u Alternative Collection: UVAC tube

TAT: 1 day Test Schedule: Daily Method: Clinitest Test Facility: AWL, KSC, Medical Office labs

TAT: 1 day Test Schedule: Daily Reducing Substance Method: Clinitest Urine Test Facility: AWL, KSC, Medical Office labs Clinical Data: n/a Patient History: n/a

Patient Preparation: Provide patient with a pre-labeled contianer and instructions for collecting "clean catch" specimen. Labeling: n/a

Processing: Refrigerate if unable to test immediately. Test within 24 hours. Transport: Transport to testing area/facility

Preferred Collection/Volume: GRN Li Hep Volume: 2.0 mL p Alternative Collection: RED SST Volume: 1.0 mL s

TAT: STAT: 1 Hour Renal Function Panel Routine: 24 Hours Test Schedule: Daily Method: See Individual Assays Test Facility: AWL, INT, SMC, LVK

Analytes include NA,K,Cl, CO2, Non-fasting GLU, BUN, Creat Alb, Calcium, and Phosphorus

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Processing: Centrifuge, DO NOT REMOVE CAP. Refrigerate

Preferred Collection/Volume: GNS5/ 2.0 mL p Alternative Collection: SSRED5/ 1.0 mL p

TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Renal Panel, Fasting Method: See Individual Assays Test Facility: AWL, INT, SMC, LVK

Patient must be fasting 10 or more hours. Analytes include Na,K,Cl, CO2, Fasting GLU, BUN, Creat Alb, Calcium, and Phosphorus

Processing: Centrifuge, DO NOT REMOVE CAP. Refrigerate

Preferred Collection/Volume: LAV EDTA Volume: 0.8 mL Plasma

TAT: 3 Days Test Schedule: Sets up 5 days a week, night Method: Liquid Chromatography Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Measurement of the Plasma Renin activity is useful in evaluating hypertension.

Specimen Stability: Plasma Renin Activity Room temperature: 24 Hours Refrigerated: Unacceptable Frozen: 28 Days -70 Degrees: Not Established Reject Thaw/Other: Thawed Plasma, Specimen stored and/or shipped refrigerated, Plasma collected in Plasma separator tube Test Code: SJC TC 16846

Processing: Centrifuge and aliquot into Referred Tests aliquot tube and freeze. DO NOT REFRIGERATE THE SAMPLE at any time. Refrigeration will cause cryoactivation to occur and prorenin will convert to renin causing falsley high renin activity results. Transport: Plasma: Transport frozen on ice preferred; Room temperature unacceptable; Refrigerated unacceptable

Research Genome NOTE: This test is for Research use only - Do Not File On This Order. Sequencing

Preferred Collection/Volume: Bronchial Lavage submitted in sterile leakproof container Volume: 1mL Bronchial Brush submitted in sterile leakproof container with saline Volume: 1mL sterile saline and brush Sputum, Endotracheal Aspirate,Tracheal Aspirate submitted in sterile leak proof container Volume: 1 mL

TAT: 2 - 5 Days Test Schedule: Daily

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Method: Culture Test Facility: Airport Way Regional Laboratory

Clinical Data: n/a Patient History: n/a

Patient Preparation: n/a Labeling: n/a Respiratory Culture Rejection Criteria: Expired collection/transport device Greater than 24 hours post collection Oropharyngeal contamination as determined by Gram Stain Bronchial brushings submitted in >1 mL sterile saline

Processing: Refrigerate if held Transport: Transport on ice

Preferred Collection / Volume: Nasopharyngeal swab submitted in UVT media Volume: 0.5 mL viral transport medium Acceptable Alternate Collection: Nasopharyngeal washing Nasopharyngeal aspirate Nasal Washing Pleural Fluid Brochoalveolar Lavage Bronchial Washings Endotracheal Aspirate Sputum

TAT: 1-3 days Test Schedule: Sunday-Saturday once per day Test Method: Reverse Transcription Polymerase Chain Reaction (PCR) Testing Location: Performed at Airport Way Regional Laboratory

Clinical Data: Respiratory Viral Panel PCR testing will only be available for Respiratory Viral Panel inpatients from both Medical Centers. This testing will be performed at the Airport Way Regional Laboratory with a turn-around-time of 1-3 days.

Rejection Criteria:

Expired collection/Transport device

Nasopharyngeal swab submitted without UVTmedia

Specimen received at ambient temperature

Tissue specimen

<0.5mL specimen

Processing: Transport specimen to Airport Way Regional Lab on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Next Day Test Schedule: Monday-Friday Morning

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Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Anti-Reticulin IgA occurs most frequently in patients with gluten-sensitive enteropathy, i.e., celiac disease, and dermatitis herpetiformis.

If the Reticulin Antibody IgA Screen is positive, the antibody titer will be added on at an additional Reticulin Antibody IgA charge (CPT: 86256). Specimen Stability: Room Temperature: 4 Days Refrigerated: 7 Days Frozen: 30 Days Test Code: CHANTILLY TC 37520

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 1.3 mL wb Alternative Collection: LAV Micro

TAT: Stat 1 hour; routine 24 hours Test Schedule: Any day, during laboratory operating hours Test Method: Sysmex XE-2100 and Sysmex XT-2000i cell counters Test Facility: AWL, KSMC and INT

Reticulocyte Count Clinical Data: n/a Automated Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Stability: Stable for 24 hrs at room temp. Stable for 72 hrs if refrigerated

Processing: n/a Transport: n/a

Preferred Collection/Volume: RED SST Volume: 0.5mL Alternative Collection: RED 10 Absolute Minimum Volume: 0.2mL

Rheumatoid Factor TAT: 24 Hours Quantitative Test Schedule: Daily Method: Turbidometry Test Facility: AWL

Processing: Centrifuge. Aliquot if RD10. Refrigerate if held

Preferred Collection/Volume: Pink EDTA Volume: 5.0 mL Alternative Collection: LAV EDTA RHIG RED 10 (do not centrifuge) Absolute Minimum Volume: 1.0 mL

Includes ABO, RH and Antibody screen, and the RHIG (Rhogam) issue. DO NOT ISSUE RHIG if Rh type is unknown, or patient is known to be RH positive. Send

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specimen to Blood Bank, KSC ORDER RHX instead of RHI if clinician requests RHIG without repeating ABO, Rh and Antibody screen; or if these tests have been completed in the last two weeks. ie: RHX if RHIG is being issued, no specimen collected. ORDER PTSC or PSC instead of RHI if clinician requests testing to be completed prior to injection. Order RHX when patient returns for injection.

Collection Instructions Preferred Collection/Volume: No specimen collection required. Laboratory staff issue RHIG to patient. Patient will obtain injection by Nurse Treatment room or OB/GYN department staff. Walk in order and obtain labels. Obtain RHIG from Rhogam Issue refrigerator. Do not open the package containing the syringe. Label the control form. Record the Lot # and expiration date, date, and sign the form. Give the patient the RHIG and part one of the form. Retain part two. Send part three to KSC.

Preferred Collection/Volume: REDSST Volume: 1.0 mL s Alternative Collection: RED10

TAT: 1-3 days Test Schedule: Monday through Friday Method: Multiplex Flow Immunoassay Test Facility: Airport Way Regional Laboratory

Clinical Data: Ribosomal P Antibody is present in 5-10% of patients with systemic lupus Ribosomal P Antibody erythematosus (SLE) Immunoassay Specimen Stability: Refrigerated : 7 days Frozen: 30 days Rejection Criteria: Gross hemolysis Gross lipemia

Processing: Centrifuge. Aliquot if collected in RED10. Transport: Refrigerate if held.

Preferred Collection: Random Urine Volume: 20 mL Minimum Volume: 5 mL

TAT: Report available 2-3 days Testing Location: Quest Diagnostics Valencia Nichols Institute Ritalin Level 27027 Tourney Road Valencia, CA 91355-5386 TC# 91923 http://www.questdiagnostics.com/testcenter/TestCenterHome.action

Processing: Transport specimen room temperature Specimen Stability: Room Temperature: 5 days(s) Refrigerated: 7 days(s)

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Frozen: 30 days(s)

Preferred Collection/Volume: DC99 (Fresh stool specimen)/ Alternative Collection: / 1.0 gram

TAT: 24 Hours Test Schedule: Daily Rotavirus Antigen Stool Method: Rapid Immunoassay Test Facility: AWL

Processing: Store fresh stool specimen refrigerated until transported. Transport on ice. Refrigerate if held overnight

Preferred Collection/Volume: RED SST Volume: 2.0 mL s Alternative Collection: RED 10 Volume: 2.0 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Method: Rapid Plasma Reagin Test Facility: AWL RPR First time positives are sent to Oregon State Lab for confirmation and FTA (see panel FTR). CSF order VDR. PTA testing continues until there is a positive.

Processing: Centrifuge Red SST. Centrifuge and aliquot if RED 10. Refrigerate if held. Transportation: Transport to AWL in blue tote

Preferred Collection/Volume: Nasopharyngeal wash/aspirate in a sterile leak proof container Alternate Collection: Nasopharyngeal swab in VTM tube

TAT: 24 hours Test Schedule: Daily Test Method: Rapid Immunoassay Testing Location: Airport Way Regional Laboratory RSV Antigen Rejection Criteria: Expired collection or transport device Sample greater than 24 hours post collection Calcium alginate swab Swab received without Viral Transport Media Specimen other than Nasopharyngeal aspirate, wash, or swab

Transportation: to AWL within 24 hours of collection

Preferred Collection/Volume: RED SST Volume: 1.5 mL s Alternative Collection: RED 10 Rubella Antibody IgG Absolute Minimum Volume: 1.0 mL s

TAT: 24 Hours Test Schedule: Mon - Sat Method: Immunoassay

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Test Facility: AWL

Test for prenatal or immune status only.

Processing: Centrifuge RED SST. Centrifuge and aliquot if RED 10. Refrigerate if held.

Preferred Collection/Volume: RED10 Volume: 1.0 mL s

TAT: Next Day Test Schedule: Monday-Friday P.M. Test Method: Immunoassay Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: sole determinant of a curent or recent infection. Because an IgM test can yield false positive results and a low level of IgM antibody may persist for more than 12 months post infection, reliance on a single test result could be misleading. If an acute infection is suspected, consider obtaining a new specimen and submit for both IgG and IgM testing in two or more weeks. Patient History: n/a

Clinical Significance Rubella is an acute exanthematous viral infection of children and adults. Rash, fever and lymphadenopathy characterize the illness. While many infections are subclinical, this virus Rubella Antibody IgM has the potential to cause fetal infection with resultant birth defects. In the absence of a current or recent infection, a demonstration of specific IgG on a serum sample is evidence of immunity to rubella. A positive rubella IgM result does not necessarily indicate current or recent infection. Without a history of exposure to rubella or symptoms consistent with rubella, the IgM result may be difficult to interpret. Rubella IgM can be false positive due to other causes (e.g., parvovirus, rheumatoid factor, cytomegalovirus). Rubella IgM may also persist for more than 12 months after vaccination or natural infection. For a serologic diagnosis of congenital rubella in the neonatal period, antibody to rubella virus should be measured in both infant and maternal sera. If IgM is detected in a newborn infants serum, it is probable that transplacental rubella infection has occurred.

Reject Criteria Gross hemolysis • Gross lipemia Specimen Stability: Room temperature: 4 days Refrigerated: 7 days Frozen: 30 days TC # 13342

Processing: Centrifuge and aliquot into Referred Tests aliquot tube Transportation: Transport to Airport Way Regional Laboratory

Preferred Collection/Volume: SSRED5/ 1.0 mL s Alternative Collection: RD10/ 1.0 mL s

TAT: 24 Hours Test Schedule: Mon - Fri

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Method: Multiplex flow Immunoassay Test Facility: AWL

Rubeola Antibody IgG For immune status only. For acute illness use Rubeola IgM Specific (RDN).

Processing: Centrifuge and aliquot if RD10. Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Next Day Test Schedule: Tuesday, Thursday Morning Method: Immunofluorescence Assay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Positive results from any one IgM assay should not be used as a sole determinant of a curent or recent infection. Because an IgM test can yield false positive results and Rubeola Antibody IgM a low level of IgM antibody may persist for more than 12 months post infection, reliance on a single test result could be misleading. If an acute infection is suspected, consider obtaining a new specimen and submit for both IgG and IgM testing in two or more weeks.

Specimen Stability: Room Temperature: 4 days Refrigerated: 7 days Frozen: 30 days Test Code: CHANTILLY TC 32762

Processing: Allow specimen to clot at room temperature and then centrifuge. Separate serum from cells as soon as possible. Aliquot 1 mL serum into a Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection: DkGRN Li Hep Alternative Collection: RED 10 Volume: 1.0 mL (Absolute Minimum Volume: 0.2 mL) Note: No gel barrier tubes

TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Salicylate Level Method: Colorimetric Test Facility: Sunnyside Medical Center and Westside Medical Center

Note: Preferred draw times: therapeutic monitoring - 1-3 hrs. post dose and anytime for suspected overdose

Processing: Centrifuge, Aliquot. Refrigerate

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 3 Days Test Schedule: Monday-Friday Morning Method: Enzyme Immunoassay Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave, Suite 200 Cypress, CA 90630

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Clinical Data: Salmonella, Total Antibody, detects antibodies to Salmonella typhii, a common cause of gastroenteritis, diarrhea, and dysentery. Antibody results should be confirmed with stool or blood cultures. Antibodies to Salmonella flagellar (H) and somatic (O) antigens typically peak 3- 5 weeks after infection. A positive result in assay is equivalent to a titer of > or = 1:160 by tube agglutination (Widal). Results should not be considered diagnostic unless confirmed by culture.

Specimen Stability: Salmonella Agglutinins Room Temperature: 7 days Refrigerated: 14 days Frozen: 30 days Test Code: CHANTILLY TC 14201 TO FOCUS TC 40450

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL whole blood

TAT: Non-priority results: 2 weeks from receipt of blood. Priority results: 7-10 days from receipt of blood Test Schedule: Monday-Saturday Test Facility: Kaiser Regional Molecular Genetics Lab 5755 Cottle Rd. Bldg #6, San Jose, CA 95123 (408)972-3308.

SCA 1 Mutation Clinical Data: Due to the fact that symptoms of SCA1 can be similar to those of Huntington disease, Machado-Joseph disease, and DRPLA, you may wish to consider testing the patient for Analysis these other neurodegenerative conditions should the SCA1 mutation analysis prove to be negative. Patient History: If applicable, and known, please include DNA results of any other affected family members

Specimen Stability: Room Temp OK Refrigerate if held overnight Send Specimen with Kaiser Molecular Request Form

Processing: Do not centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL whole blood

TAT: Non-priority results: 2 weeks from receipt of blood. Priority results: 7-10 days from receipt of blood Test Schedule: Monday-Saturday Test Facility: Kaiser Regional Molecular Genetics Lab 5755 Cottle Rd. Bldg #6, San Jose, CA 95123 SCA 2 Mutation (408)972-3308. Analysis Clinical Data: Due to the fact that signs and symptoms of SCA2 can be similar to those of SCA 1, Machado-Joseph (SCA3) disease, Huntington disease and DRPLA, you may wish to consider testing the patient for these other neurodegenerative conditions should the SCA2 mutation analysis prove to be negative. Patient History: If applicable, and known, please include DNA results of any other affected family members

Specimen Stability: Room Temp OK Refrigerate if held overnight Send Specimen with Kaiser Molecular Request Form

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Processing: Do not centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL whole blood

TAT: Non-priority results: 2 weeks from receipt of blood. Priority results: 7-10 days from receipt of blood Test Schedule: Monday-Saturday Test Facility: Kaiser Regional Molecular Genetics Lab 5755 Cottle Rd. Bldg #6, San Jose, CA 95123 (408)972-3308.

Clinical Data: Due to the fact that symptoms of MJD can be quite variable and can be similar to SCA 3 DNA Mutation those of Huntington disease, Spinocerebellar Ataxia Type 1 (SCA1), or DRPLA, you may wish to Analysis consider testing the patient for these other neurodegenerative disorders should the MJD1 mutation analysis prove to be negative Patient History: If applicable, and known, please include DNA results of any other affected family members

Specimen Stability: Room Temp OK Refrigerate if held overnight Send Specimen with Kaiser Molecular Request Form

Processing: Do not centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL whole blood

TAT: Non-priority results: 2 weeks from receipt of blood. Priority results: 7-10 days from receipt of blood Test Schedule: Monday-Saturday Test Facility: Kaiser Regional Molecular Genetics Lab 5755 Cottle Rd. Bldg #6, San Jose, CA 95123 (408)972-3308.

Clinical Data: Due to the fact that signs and symptoms of SCA6 can be similar to those of the SCA 6 Mutation other SCA disorders, DRPLA and sometimes Huntington disease, you may wish to consider testing Analysis the patient for some or all of these other neurodegenerative conditions should the SCA6 mutation analysis prove to be negative. Patient History: If applicable, and known, please include DNA results of any other affected family members

Specimen Stability: Room Temp OK Refrigerate if held overnight Send Specimen with Kaiser Molecular Request Form

Processing: Do not centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL whole blood

TAT: Non-priority results: 2 weeks from receipt of blood. Priority results: 7-10 days from receipt of blood Test Schedule: Monday-Saturday

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Test Facility: Kaiser Regional Molecular Genetics Lab 5755 Cottle Rd. Bldg #6, San Jose, CA 95123 (408)972-3308.

Clinical Data: Due to the fact that signs and symptoms of SCA7 can be similar to those of the other SCA disorders, DRPLA and sometimes Huntington disease, you may wish to consider testing the patient for some or all of these other neurodegenerative conditions should the SCA7 mutation analysis prove to be negative. SCA 7 Mutation Patient History: If applicable, and known, please include DNA results of any other affected Analysis family members

Specimen Stability: Room Temp OK Refrigerate if held overnight Send Specimen with Kaiser Molecular Request Form

Processing: Do not centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume: Skin scrapings: Place on a glass slide and cover with mineral oil. Place a second slide over the top of the specimen. Tape the two slides together and transport the specimen slides in a petri dish or sterile leakproof container. Alternative Collection: n/a

TAT: 1 day Test Schedule: Daily Method: Microscopy Test Facility: AWL, BVT, CPK, DIV, INT, LVK, NLR, ORC, RKW, SAL, SKY Scabies Identification Clinical Data: n/a Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: Room Temperature Transport: Room Temperature

Preferred Collection/Volume: LAV EDTA Volume: 2mL wb Alternative Collection: LAV Microtainer

TAT: STAT: 4 hours Routine: 24 hours Test Schedule: 7 days per week Test Method: Manual count by Miller Disc Method Testing Location: Airport Way Regional Laboratory Schisto Cnt Wet/Dry Microscopy

Stability: Room Temperature: 8 hours Refrigerated: 24 hours Rejection Criteria: Clotted specimens Hemolyzed specimens IV contaminated specimens

Processing: DO NOT centrifuge

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Transportation: Transport to AWL in blue tote

Preferred Collection/Volume: REDSST Volume: 1.0 mL s Alternate Collection: RED10

TAT: 1-3 days Test Schedule: Monday through Friday Method: Multiplex Flow Immunoassay Test Facility: Airport Way Regional Laboratory

Clinical Data: Scl-70 antibody is present in approximately 40% of patients with Progressive Scl 70 Antibody Systemic Sclerosis (PSS).

Specimen Stability: Refrigerated: 7 Days Frozen: 30 Days Rejection Criterion: Gross hemolysis Gross lipemia

Processing: Centrifuge. Aliquot if collected in RED10 Transport: Refrigerate if held.

Preferred Collection/Volume: Black Cit Volume: 2 mL Alternative Collection: LAV EDTA

TAT: Stat 1 hour; routine 24 hrs Test Schedule: Any day, during laboratory operating hours Test Method: Westergren Method Test Facility: AWL, KSMC, INT. LVK, NLR

Stability:

Sedimentation Rate { BLK-ESR tube is stable for 4 hrs at room temperature. { BLK-ESR tube is stable for 24 hrs when refrigerated

Note : Test may be added to room temp EDTA specimens within 4 hrs or refrigerated specimens within 12 hrs. Rejection Criteria:

{ Under-filled or over-filled BLK-ESR tubes

Processing: Refrigerate until shipped

Preferred Collection/Volume: Dark Blue Serum Volume: 2 mL serum Alternative Collection: Dark Blue EDTA Volume: 2 mL Plasma

TAT: 2 days Test Schedule: Tuesday, Friday a.m. Method: Atomic Spectrometry (AS) Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

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Clinical Data: Selenium is an element of parenteral nutrition. Monitoring the Selenium concentration is useful in assessing parenteral nutrition, especially recent intake. Concentrations are also monitored in children with propionic acidemia who require special diets with supplements

Specimen Stability: Room temperature: 8 Hours Refrigerated: 14 Days Frozen: 30 Days Selenium Level Reject Criteria: Received room temperature; Hemolysis Test Code: CHANTILLY TC 5507

Processing: Centrifuge serum or plasma specimen within 1 hour of collection. Immediately separate serum or plasma from the cells into trace metal free aliquot tube. Note on sample if serum or plasma. Transport: Refrigerate if held.

Preferred Collection/Volume: Avoid worksite collection Random Urine in Trace metal free collection container. Use acid washed aliquot containers attached to the acid washed 24 urine containers Volume: 7.0 mL Urine

TAT: 3 Days Test Schedule: Tuesday, Friday Morning Method: Inductively Coupled Plasma-Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute Valencia 27027 Tourney Road Valencia, CA 91355-5386

Clinical Data: Selenium is considered an essential trace element. It enters the food chain Selenium Level Urine through plants. Selenium plays an important role in the control of thyroid hormone metabolism. Selenium deficiency may cause reduced growth rates, owing to a feedback response, which lowers tri-iodothyronine-mediated synthesis of growth hormone in the pituitary, while a combined deficiency of selenium and iodine exacerbates hypothyroidism. Selenium is important for proper reproductive performance.

Specimen Stability: Room Temperature: 5 days Refrigerated: 14 days Frozen: 30 days Test Code: CHANTILLY TC 16867 TO VALENCIA TC 4875UR

Transport: Room Temperature

Preferred Collection/Volume: Clean container. Usually a conical centrifuge tube prepared at collection site. Entire specimen needed for testing. Includes smears prepared at processing site. Alternative Collection: N/A

TAT: 2 days Test Schedule: Daily Semen Analysis 2nd Method: Microscopy Part Test Facility: AWL only

Preparation: Semen analysis 2nd part. Refer to Semen Analysis, Complete. Labeling: N/A

Processing: 2nd part. Includes sperm count (sperm/mL and total) and morphology. Transport: See 1st part.

Preferred Collection/Volume: AP99 or clean container. Entire specimen needed

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for testing.

TAT: 1 day Test Schedule: Daily Method: Microscopy Test Facility: Medical Office labs, SMC, WMC

Patient Preparation: Provide patient with a pre-labeled container and specific Semen Analysis collection instructions. Complete Processing: If dropped off, document time of collection. Initial testing must be perfromed immediately upon receipt, or within 1 hour of collection. Smears for sperm morphology are prepared on site. 1st part includes volume, motility, progressive motility. Transport: Upon completion of initial exam (1st part), send all remaining specimen and smears to AWL for 2nd part.

Preferred Collection: AP99 or clean container Volume: Entire specimen is needed for testing.

TAT: 1 day Testing Schedule: Daily Method: Microscopy Semen Analysis Post Testing Location: BVN, CPK, INT, GTW, LVK, ORC, NLR, RKW, SAL, SKY, Vasectomy SMC, TUA, WMC

Patient Preparation: Provide patient with a pre-labeled container and specific collection instructions.

Processing: Tested on site. Test within 1 hour of collection. For sites without testing transport immediately to nearest testing location as per routing on label.

Preferred Collection: RED 10 Alternative Collection: RED SST

Volume: 2 mL

Minimum Volume:

TAT: Report available with in 6 days Test Schedule: Monday and Wednesday Testing Location: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Significance:

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The presence of antibodies to the gangliosides GM1, Asialo-GM1, and GD1a has been associated with motor neuropathies. High titers of antibody to GM1 are most typical of multifocal motor neuropathy; however, antibodies to other gangliosides are often present. In most cases, anti-ganglioside antibodies are IgM class. IgG antibodies have been reported less often, generally associated with acute conditions. Many reports have found increased anti-asialo-GM1 antibody titers in patients with multifocal motor neuropathy, lower motor neuron disease, or motor neuropathy with or without multifocal block, Guillain-Barré Syndrome, and amyotrophic lateral sclerosis. There may be a relationship between titers of asialo- GM1 antibodies and clinical status in individual patients. In some lower motor neuron disorders, improvement in strength has occurred after therapeutic reduction in anti-asialo-GM1 antibody titers.

Includes: Sensory-Motor Ganglioside GM-1 Antibodies (IgG, IgM), EIA; Ganglioside GD1a Antibody (IgG), Neuropathy Ab Panel EIA; Ganglioside GD1a Antibody (IgM), EIA; Ganglioside Asialo-GM-1 Antibody (IgG), EIA; Ganglioside Asialo-GM-1 Antibody (IgM), EIA

Specimen Stability Room Temperature: 24 hours Refrigerated: 7 days Frozen: 30 days Rejection Criteria: Specimens received room temperature TC# 90131

Processing: Centrifuge specimen and aliquot into Referred Test aliquot tube, and Freeze Transportation: Send frozen specimen on ice and transport to Airport Way Regional Laboratory

Preferred Collection/Volume: Serotonin Kit is available from Materials, X35-6765 for blood panel. LAV EDTA to Serotonin Kit Volume 4.0 mL Whole Blood.

TAT: 2 Days Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Serotonin Level Patient Preparation: Patient should avoid foods high in indoles: avocado, banana, tomato, plum, walnut, pineapple, and eggplant. Patient should also avoid tobacco, tea, and coffee for 3 days before specimen collection.

Specimen Stability: Room Temperature: Unacceptable Refrigerated: Unacceptable Frozen: 6 Weeks Test Code: CHANTILLY TC 9651

Processing: Mix specimen well and transfer immediately to a plastic bottle containing 35 mg Ascorbic Acid. Mix well and freeze immediately. Transport: Transport frozen on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 3.0 mL Serum

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Alternative Collection: LAV EDTA Volume: 3.0 mL Plasma

TAT: 4 Days Test Schedule: Monday-Thursday, Sunday Morning Method: Gas Chromatography Test Facility: National Medical Services 3701 Welsh Rd Willow Grove, PA 19090

Clinical Data: Sertraline is an antidepressant used in the treatment of mental depression. Sertraline Level Monitoring its level is important to optimize therapy, to avoid toxicity, and to assure compliance.

Specimen Stability: Room Temperature: 1 week Refrigerated: 20 days Frozen: 18 months Rejection Criterion: Polymer gel separation tube (SS or PST). Test Code: CHANTILLY TC 4146 TO NMS TC 4195SP

Processing: Centrifuge serum or plasma specimen within 1 hour of collection. Immediately separate serum or plasma from the cells into special trace element collection vial(s). Transport: Refrigerate if held.

Preferred Collection/Volume: RED10 Volume: 1.0 mL s Absolute Minimal Volume: .5mL s

TAT: Next Day Test Schedule: Mon-Fri Evening Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: The hormones testosterone, dihydrotestosterone and estrogens are bound to SHBG. SHBG concentrations are increased with pregnancy, birth control pills, and decreased with Sex Hormone Binding testosterone and obesity. Changes in SHBG will influence the total hormone concentration but not Globulin the free hormone concentrations by dialysis. Calculated bioavailable testosterone, while immune to minor changes in SHBG, may be biased by extremely elevated concentrations such as in pregnancy.

Specimen Stability: Room temperature: 7 days Refrigerated: 7 days Frozen: 60 days Reject Criteria : Plasma; Gross hemolysis TC# 30740

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Transport to AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: 2 mL or more EDTA specimen Alternative Collection: n/a

TAT: 1-2 working days Sezary Cell Smear Test Schedule: Monday - Friday Test Method: n/a Test Facility: Airport Way Laboratory

Clinical Data: n/a

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Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: n/a Transport: Deliver specimen to WET/DRY microscopy at AWL

Preferred Collection/Volume: LV5/ 5.0 mL WB Alternative Collection: LV3 acceptable/ 1.0 mL WB

TAT: 1 Week Test Schedule: Once a week (Thursday/Friday) Sickle Cell Solubility Method: Solubility Test Test Facility: AWL

Specimen refrigerated up to 4 weeks is acceptable

Processing: Do not centrifuge. Refrigerate

Preferred Collection/Volume: Sinus Aspirate placed in sterile leakproof container or syringe (without needle) / 1 mL Alternative Collection: Nasopharyngeal swab: Liquid Amies Transport swab (E-Swab)

TAT: 2 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: n/a Patient History: n/a Sinus Culture Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired collection/transport device. Greater than 24 hours post collection for aspirates. Greater than 48 hours post collection for E-swabs. Dry Swab.

Processing: Room Temperature Transport: Room Temperature

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL wb

TAT: Test Schedule: Monday through Friday Test Method: Immunoassay Test Facility: Airport Way Regional Laboratory Sirolimus Level Clinical Data: Sirolimus is an immunosuppressant drug used to prevent organ graft rejection. Therapeutic drug monitoring is used to optimize dose and avoid toxicity.

Patient Preparation: Optimal time for collection is 1/2 hour to 1 hour before next oral dose at steady state.

Specimen Stability: Room Temperature : N/A

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Refrigerated: 1 Week Frozen: 6 Months

Sirolimus (Rapamycin)

Processing: Do not centrifuge. Transport: Transport to AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: REDSS Volume: 1mL s Alternative Collection: RED 10 Volume: 1.0 mL serum

TAT: 1-3 days Test Schedule: Monday through Friday Method: Multiplex Flow Immunoassay Test Facility: Airport Way Regional Laboratory

Clinical Data: Sjogren's Antibody (SS-B) is detected in approximately 15% of patients with Sjogren's syndrome. Sjogren's Antibody (SS-B) is present only if Sjogren's Antibody (SS-A) is also Sjogren's Antibody detected. The presence of both antibodies (SS-A and SS-B) strengthen the diagnosis of Sjogren's syndrome and conveys prognostic information.

Specimen Stability: Refrigerated: 7 days Frozen: 30 days Rejection Criteria: Gross Hemolysis Gross Lipemia

Processing: Centrifuge. Aliquot serum if collected in RED10 Transport: Refrigerate if held.

Preferred Collection/Volume: Green 3 Sodium Heparin/ 3cc MINIMUM VOLUME 1CC Alternative Collection: n/a

TAT: Routine: 14-21 days Test Schedule: Mon-Fri Method: In situ hybridization on metaphase cells Test Facility: AWL

Smith Magenis Clinical Data: If ordered in combination with a chromosome study, only 1 specimen is needed. Mutation (17p11.2) Include clinical indication. Analysis FISH Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: ** No Gel barrier tubes ** Do not Centrifuge. Hold at Room temperature. Do NOT reject centrifuged specimen; contact department at 503-258-6723 (Tie Line: 35-6723) Transport: Room Temperature.

Preferred Collection/Volume: RD10/ 2.0 mL s Alternative Collection: Smooth Muscle Antibody Screen TAT: 24 Hours Test Schedule: Mon - Fri Method: Immunofluorescence

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Test Facility: AWL

Processing: Cent, Pour, Refrigerate LAB CENT: Cent, Pour, Refrigerate

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5 mL p/s Alternative Collection: RED SST or serum acceptable Absolute Minimum Volume: 0.2 mL p/s

TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Sodium Level Method: iStat: ISE Potentiometry Test Facility: AWL, SMC, INT, BVT, CAS, LVK, SAL, NLR, RKW, SKY, VAN, DIV, SST, TUA, ORC

iSTAT Testing Performed by LAB Staff: Whole blood from unspun GNS5 acceptable for testing

Processing: Centrifuge. Refrigerate if held

Preferred Collection/Volume: 24 hr Urine (no preservative)/ 4.0mL u Alternative Collection: 12x75 aliq./ 1.0mL u

TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Sodium Level 24 Hour Method: ISE Urine Test Facility: AWL, SMC

Refrigerate during collection. Preservatives are not acceptable. See panel RNA for random specimen

Processing: Record collection interval hrs. Measure and record total vol. in ARE in LIS. Aliquot to 12x75 tube. Refrigerate

Preferred Collection/Volume: AP99 with 12x75 aliq./ 4.0mL u Alternative Collection: / 1.0mL u

TAT: STAT: 1 Hour Routine: 24 Hours Sodium Level Urine Test Schedule: Daily Method: ISE Test Facility: AWL, SMC

Processing: Aliquot to 12x75 tube. Refrigerate

Preferred Collection: Heparinized syringe Alternative Collection: Dk Grn Li Hep Volume: 3.0 mL whole blood (Absolute Minimum Volume: 0.2 mL whole blood)

TAT: 1 Hour Sodium Whole Blood Test Schedule: Daily Method: Ion Selective Electrode Test Facility: Sunnyside Medical Center and Westside Medical Center

Processing: Needle must be removed and syringe capped. Analyze within 30 minutes of collection. Transport: Transport at room temperature

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Preferred Collection/Volume: At least 2 mLs of random urine in AP99 or UVAC (no preservative).

TAT: 1 day Test Schedule: Daily Method: Refractometer Test Facility: AWL, SMC, WMC, Medical Office labs Specific Gravity Urine Patient Preparation: Provide patient with pre-labeld container. Clean catch not necessary if only test ordered.

Processing: Refrigerate if not tested within 2 hours. Must be tested within 24 hours. Transport: Transport refrigerated to testing facility.

Preferred Collection/Volume: CSF collection tube/2 ml (All volumes accepted). Alternative Collection: Any extra tube or specimen (not sure about this portion).

TAT: 24 - 48 hr Test Schedule: Mon-Fri Method: Microscopic review Test Facility: AWL

Clinical Data: Enter releveant clinical information. Patient History: Enter relevant patient history. Specimen Tracking CSF Cytology Patient Preparation: n/a Labeling: Label with first and last name and health record number.

Specimens are not rejected but sent to AWL for assessment. AWL receive specimens, build and result worklist.

Processing: Concentrate by centrifugation (if possible prior to) preparing two cytospin slides on-site. Do not delay sending specimen to Cytology for processing. Transport: Ensure containers are secure and properly sealed. Send Health Connect or other requisition indicating patient demographics and pertinent clincal history with specimen.

Preferred Collection/Volume: PAP Smear/Bottle/Vial Alternative Collection: SurePath (blue top) container. 50

TAT: 14 days Test Schedule: Mon-Fri Method: Microscopic review Test Facility: AWL

Specimen Tracking Gyn Clinical Data: Enter specimen site, last menstrual history and relevant clinical Cytology information. Patient History: Indicate any history of Gyn abnormality, hormone treatment and or chemotherapy.

Patient Preparation: n/a Labeling: Label with first and last name and health record.

Specimens are not rejected but sent to AWL for assessment. AWL receive specimens, build and result worklist.

Processing: Tracking Panel for AWL specimens. Room temperature if held.

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Transport: Apply 1 label to pap specimen container and 1 to Health Connect requisition. Do not cover the patient information on the specimen container. Send Health Connect order or other requisition with specimens.

Preferred Collection/Volume: Clean, sterile, leakproof container. Alternative Collection: Clean sterile specimen container with saline or any of the following fixative solutions. Blue cytology solution (95% alcohol), ThinPrep Cytolyte, or ThinPrep PreserveCyte container.

TAT: 24 -48 hr Test Schedule: Mon-Fri Method: Microscopic review Test Facility: AWL

Clinical Data: Enter relevent clinical information. Specimen Tracking Patient History: Enter relevant patient history. Non-Gyn Cytology Patient Preparation: n/a Labeling: Label with first and last name and health record number.

Specimens are not rejected but sent to AWL for assessment. AWL receive specimens, build and result worklist.

Processing: Concentrate by centrifugation (if possible prior to) preparing two cytospin slides on-site. Do not delay sending specimen to Cytology for processing. Transport: Ensure containers are secure and properly sealed. Send Health Connect or other requisition indicating patient demographics and pertinent clincal history with specimen.

Preferred Collection/Volume: Clean, sterile, leakproof container/50-100 ml Alternative Collection: 50% ethyl alcohol equal volume of specimen.

TAT: 24-28 hr Test Schedule: Mon-Fri Method: Microscopic review Test Facility: AWL

Clinical Data: n/a Specimen Tracking Patient History: n/a Urine Cytology Patient Preparation: n/a Labeling: n/a

24 hour old specimens are rejected. AWL receive specimens, build and result worklist.

Processing: Refrigerate if held within 24 hour period. Tracking Panel for AWL specimens. Transport: Ensure containers are secure and properly sealed. Send Health Connect or other requisition indicating patient demographics and pertinent clinical history with specimen.

Preferred Collection/Volume: Sterile container. Suggest Baxter cat. # C-8827-4. Patient delivers specimen to OB-lab within 45 min. of collection. Alternative Collection: Frozen doner sperm in nitrogen container brought in by patient. Sperm Count and Motility, Artificial TAT: 1 day Insemination Test Schedule: Daily, By appt. time Method: Microscopy Test Facility: EIN-OB

Patient Preparation: Appointment time and specimen collection instructions are

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provided by OB department. Labeling:

Processing: Tested immediately upon receipt at EIN-OB IUI lab. Transport:

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 1 Day Test Schedule: 1 Day a week, a.m. Method: Immunobeads Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Sperm antibodies are detected in approximately 15-20% of men with otherwise Spermatozoa Antibody unexplained infertility and in 10-15% of women with otherwise unexplained infertility.

Specimen Stability: Room temperature: 24 Hours Refrigerated: 14 Days Frozen: 14 Days Reject Criteria: Received room temperature Test Code: SJC TC 19492X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL whole blood

Test Schedule: MON-FRI with completed TPMG Molecular requisition Test Facility: Kaiser Regional Molecular Genetics Lab 5755 Cottle Rd. Bldg #6, San Jose, CA 95123 Spinal Muscular Atrophy DNA Analysis Specimen Stability: Room Temp OK Refrigerate if held overnight Send Specimen with Kaiser Molecular Request Form

Processing: Do not centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume: Spordi Test Kit Alternative Collection: n/a

TAT: 7 - 10 Days Test Schedule: Daily Method: Culture Spordi Test Test Facility: AWL Clinical Data: n/a Patient History: n/a

Patient Preparation: n/a Labeling: Label envelope with pertinent information

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Rejection Criteria: Expired collection/transport device

Processing: Room Temperature Transport: Room Temperature

Preferred Collection/Volume: Patient Hardware in sterile leakproof container Alternative Collection: n/a

TAT: 2 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: This culture is for patient hardware. Test is for growth/no growth only. Susceptibilities will not be performed. Sterility Culture Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Greater than 24 hours post collection Non-patient sample

Processing: Room Temperature Transport: Room Temperature

Preferred Collection/Volume: Kidney Stone: Sterile Container, leak-proof Non-Kidney Stone: Sterile Container, leak-proof Minute specimens may be placed in a gelatin capsule. Minimum Volume: N/A

TAT: 5 Days Test Schedule: Monday-Saturday Morning Method: Gravimetric Fourier Transform Infrared Spectroscopy (FTIR) Test Facility: Quest Diagnostics Nichols Institute Valencia 27027 Tourney Road Valencia, CA 91355-5386

Stone Analysis Infrared Clinical Data: Stone analysis is used in determining the etiology of stones. The results are Spectroscopy often useful in determining the cause and treatment. Labeling: Source/Type of stone is REQUIRED for all submissions.

Specimen Stability: Room Temperature: 12 Months Refrigerated: 12 Months Frozen: 12 Months Test Code: CHANTILLY TC 30260 TO VALENCIA TC 4161

Processing: Stones originating from sources not related to the kidney should be air-dried, then placed in a plastic tube or a urine collection cup. Do not use tape. Transport: Refrigerate if held.

Preferred Collection/Volume: Orange Para Volume: Add > 2 grams Fresh Stool Specimen to Orange Top Para-Pak C&S container within one hour of collection. Alternative Collection: Rectal Swab: Liquid Amies Transport swab (E-Swab), or fresh stool delivered to laboratory within one hour of collection or refrigerated for up to 24 hours.

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TAT: 3 - 7 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: This test code is for routine stool culture (rules out Salmonella, Shigella, Campylobacter, E. coli 0157, and Shiga toxins 1 & 2). To rule out Yersinia, order Yersinia Culture. To rule out Vibrio, order Vibrio Culture. Patient History: n/a

Patient Preparation: n/a Stool Culture Labeling: n/a

Rejection Criteria: Enteric Pathogen vial > 72 hours post collection Fresh (refrigerated within one hour of collection) stool > 24 hours post collection Rectal Swab 48 hours post collection Expired collection/transport device Specimen contaminated with urine Specimen received in diaper

Processing: Refrigerate Transport: Send on Ice

Preferred Collection: Red 10 Alternative Collection: Red SS Minimum Volume: 0.25 mL

TAT: Report available within 3 days of specimen receipt at the testing location. Testing Location: Focus Diagnostics (via Quest San Juan Capistrano) 5785 Corporate Ave, Suite 200 Cypress, CA 90630 Strep Pneumo IgG, 23 TC# 16963 Serotypes http://www.questdiagnostics.com/testcenter/TestCenterHome.action Stability: Room Temperature: 7 days Refrigerated: 14 days Frozen:90 days

Processing: Centrifuge specimen and aliquot serum into a Referred Tests aliquot tube. Transportation: Transport in a thermos, at room temperature, in a Blue tote to Airport way laboratory to the Referred Tests department.

Preferred Collection/Volume: Swab throat using BBL CultureSwab dual swab Liquid Amies Alternative Collection: n/a

TAT: <1 Day Test Schedule: Daily Method: Antigen Detection Test Facility: BVT, CPK, DIV, INT, SMC, LVK, NLR, ORC, RKW, SAL, SKY, SST, TUA, VAN,

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WSM, MHO, HBN

Clinical Data: If patient is 15 years old or younger, and Rapid Strep test is negative, a Beta Hemolytic Streptococcus Culture will be reflexed. Patient History: n/a

Swab throat using BBL CultureSwab dual swab Liquid Amies

Strep Test Rapid Rejection Criteria: Greater than 24 hours post collection Expired collection/transport device Dry Swab Source other than throat/tonsil

Processing: Room Temperature Transport: Room Temperature

Preferred Collection/Volume: Two vaginal/rectal co-collection swabs. Alternative Collection: One vaginal/rectal co-collection swabs.

TAT: 1-3 days Test Schedule: Mon - Fri Method: Real Time PCR Test Facility: AWL: Molecular Diagnostics

Streptococcus Group B Clinical Data: Streptococcus Group B OB PCR is for OB Patients ONLY. Group B OB DNA Amplified Step DNA Detected or Indeterminate are automatically reflexed for susceptibility Probe w Reflex To testing if they are known to be penicillin and/or cephalosporin allergic. Culture and Sensitivity Patient History: Information about penicillin and/or cephalospoin allergy is required: YES, NO or UNSURE. Gestational Age is required.

Preparation: Specimen:VAGINAL/RECTAL co-collected swab(s) ONLY. Labeling: N/A

Processing: Collect vag/rec swab and place in Amies clear gel, Copan Transystem Liquid Stuart Medica, or BD ESwab Transport System (flocked swabs Transport: Transport vag/rec swab within 24 hours of collection at 2 - 30 C

Specimen Collection: Random urine in a leak proof urine collection container.

TAT: 2 hours (after received in laboratory) Testing Schedule: Daily Testing Locations: Sunnyside Medical Center and Westside Medical Center Specimen Stability: Streptococcus Room Temperature: 24 hours Pneumonia Ag, Urine Refrigerated: 14 days Frozen: 14 days Rejection Criteria: Any specimen other than urine.

Transport: Deliver to laboratory as soon as possible. Medical Center Laboratory: Refrigerate specimen if testing will be delayed longer than 24 hours from collection.

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Preferred Collection/Volume: RED 10 Volume: 1mL Serum

TAT: 3 days Test Schedule: Monday-Friday Morning Method: Immunofluorescence Assay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway Striated Muscle San Juan Capistrano, CA 92690-6130 Antibody Screen specimen stability: Room Temperature: 4 days Refrigerated: 14 days Frozen: 30days (-20c) Test Code: Quest TC 266

Processing: Centrifuge and aliquot into a referred tests aliquot tube Transport: Refrigerate if held.

Preferred Collection/Volume: Preferred Specimen: Random Pre-shift or End of shift Urine Volume: 2.0 mL

TAT: [Colorimetry] Friday 1 day [IC]; Monday Thursday 2 days Test Schedule: [Colorimetry] Friday [IC]; Monday Thursday Method: Colorimetry (C) Ion Chromatography (IC) Test Facility: NMS Labs 3701 Welsh Road Willow Grove, PA 19090 Styrene Exposure Profile Urine Specimen Stability: Room Temperature: Undetermined Refrigerated: Undetermined Frozen (-20 °C): Undetermined Failure to submit a frozen sample will result in cancellation. Test Code: NMS TC 4213U

Processing: Collect sample at end of shift. Freeze . Transport: Transport frozen on ice

Preferred Collection/Volume: Sealed container

TAT: 48 Hours Test Schedule: Daily Method: GC/MS Substance Analysis Test Facility: AWL

For analysis of unknown substances, e.g. pills, powders, syringe contents. If syringe, do NOT send needle

Processing: none required. Send to Toxicology

Preferred Collection/Volume: LAV EDTA Volume: 1.0 mL Plasma Alternative Collection: RED 10 Volume: 1mL Serum NO RED SST

TAT: 4 Days Test Schedule: Tuesday, Thursday Morning Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: National Medical Services

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3701 Welsh Rd Willow Grove, PA 19090

Specimen Stability: Room Temperature: 7 days Refrigerated: 7 days Sulfonylurea Frozen: 4 months Hypoglycemics Serum Test Code: Quest TC 4425

Processing: Centrifuge and and aliqout serum or plasma into a Referred Tests aliquot tube. Note if serum or plasma on specimen. Transport: Refrigerate if held

Preferred Collection/Volume: ***No GEL Barrier*** One green 4 Sodium heparin; One LV5 Alternative Collection: n/a

TAT: 1 – 3 days Test Schedule:

{ Monday – Friday: Send to AWL { Saturday/holidays: Cab specimen directly to OHSU from collection site. order as RZZ when sending to OHSU. { Sunday: Send to AWL

Test Method: BD FACSCanto II Flow Cytometer Test Facility: AWL, Flow Cytometry

A CBC is needed for RTB. If there is no same day CBC, contact the ordering Surface Marker clinician to order a routine CBC also. Tracking Clinical Data: n/a Patient History: n/a

Patient Preparation: n/a Labeling: n/a

{ Complete the hematopathology history form { List diagnosis or R/O in tracking and on the hematopathology history form

Note : A pathologist or clinician may instruct laboratory staff to cab specimen to OHSU directly from collection site. Follow the instructions and order the test as RZZ.

Processing: Keep at room temperature. Do not centrifuge. Transport: Transport at room temperature when sending to Airport Way Regional Laboratory.

Preferred Collection/Volume: Aspirate in flow cytomerty BM media Alternative Collection: n/a

TAT: 1 – 3 working days Test Schedule:

{ Monday – Friday: Send to AWL { Saturday/holidays: Cab specimen directly to OHSU from collection site. order as RZZ when sending to OHSU. { Sunday: Send to AWL

Method: BD FACSCanto II Flow Cytometer

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Test Facility: AWL, Flow Cytometry

Clinical Data: n/a Patient History: n/a

Patient Preparation: Clinician explains the procedure to the patient before specimen collection. Labeling: n/a Surface Marker { Complete the hematopathology history form Tracking Bone Marrow { List diagnosis in tracking and on the hematopathology history form

Note : A pathologist or clinician may instruct laboratory staff to cab specimen to OHSU directly from collection site. Follow the instructions and order the test as RZZ.

Processing: Do not centrifuge. Keep at room temperature Transport: Transport at room temp

Preferred Collections: Tissue placed in RPMI media (Blue top) CSF/Body fluid placed in a RPMI media (Blue top) Volume: 1 mL or more Minimum Volume: 0.5 mL

TAT: 1-3 days Testing Schedule: Sunday-Friday send specimens to Airport way. Saturday and Holidays cab specimen directly to OHSU from collection site (order Surface Marker as RZZ when sending specimens to OHSU). Tracking Tissue Testing Method: BD FACSCanto II Flow Cytometer Testing Location: Airport way Flow Cytometry department

A pathologist or clinician may instruct laboratory staff to cab specimens to OHSU directly from collection site. Follow the instructions and order the test as RZZ. Note: To obtain RPMI media call 35-6891 (Histology/Pathology). Refer to Work Instruction 2.2-15-205 for further details.

Processing: Do not centrifuge body fluids. Transportation: Transport specimen room temperature, in a Blue tote to Airport Way Laboratory.

Preferred Collection/Volume: Blood in EDTA, Bone Marrow in EDTA, Paraffin Embedded Tissue, Fresh Tissue in RPMI Media Volume: Blood: 10mL wb Bone Marrow: 1-2mL

TAT: Test Schedule: Test Method: Testing Location: University of Washington Medical Center Hematopathology Laboratory, G7-800

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Seattle Cancer Care Alliance 825 Eastlake Avenue E. Seattle, WA 98109 After hours contact the hemepath fellow on call: 206-598-6190 Phone Numbers: Flow Cytometry Hematopathology: 206-288-7060 Molecular Hematopathology: 206-288-7070 Fax Numbers: Flow Cytometry Hematopathology: 206-288-7127 Molecular Hematopathology: 206-288-7126

T-cell clonality Monoclonal populations of T lymphocytes can be identified by testing for T cell receptor γ chain (TCRγ) gene rearrangements using a polymerase chain reaction assay. In its germline configuration the TCRγ gene consists of 6 functional V gene segments, 8 V pseudogene T Cell Clonality by PCR segments and 5 J segments. There is enough homology among the V region segments and among the J region segments to allow use of small numbers of consensus primers to amplify across the V-J join N region. A polyclonal T cell population will produce a Gaussian distribution of PCR products on capillary electrophoresis, while a clonal process will produce a discrete peak. Approximately 95% of T cell lymphomas produce a PCR product using our primer set. In a mixed population of T lymphocytes, a neoplastic clone must represent about 1% of the total T cells in order to detect a clonal peak in a polyclonal distribution of background peaks.

Pathologist will comlete requisition. Send CLINICALHEMATOPATHOLOGY LAB REQUEST UW MEDICINE REFERENCE LABORATORY SERVICES and specimen refrigerated if blood or bone marrow. If frozen tissue, do not allow to thaw.

Processing: Do not centrifuge Transport: Transport to AWL

Preferred Collection: LAV EDTA Minimum Volume: 3mL wb

TAT: 48hrs Test Schedule: Monday - Friday

Note: A CBC must be accompany this test. T Cells Absolute CD4 and CD8 Note: Specimens must be collected by 1200PM (noon) on Fridays. All other days and times are acceptable for specimen collection.

Note: If CBC is not ordered, contact clinician to order a routine CBC.

Processing: Do not centrifuge Transport: Transport specimen at room temperature

Preferred Collection/Volume: Swab in conical tube with a few drops of saline added. Test immediately

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TAT: 1 hour Test Schedule: Daily Method: Microscopy Test Facility: Medical Office labs, SMC, WMC

Patient Preparation: Clinician collection

A disclaimer will be reported with results if specimen tested more than one hour post-collection. T+Y Wet Mount TYS is for female patients ONLY. If clinician requests Trichomonas evaluation for a male patient, order a STAT Urine Microscopic. Notify Testing lab that clinician is evaluating for Trichomonas. To evaluate the male patient for yeast, order a Fungal Culture on urine.

Processing: Transport at room temperature as soon as possible to testing lab. For best results, testing should occur within one hour of collection. Transport: Transport at room temperature as soon as possible to testing lab.

Preferred Collection/Volume: SSRED5/ 1.5mL s Alternative Collection: RD10, / 1.0mL s GNS5 plasma

TAT: 24 Hours T3 Total Test Schedule: Mon - Fri Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, Aliquot if RD10, and Refrigerate if held

Preferred Collection/Volume: LAV EDTA Draw 12 hours after last dose or at trough or as directed by clinician or pharmacist Volume: 5 mL wb

TAT: 24hours Test Schedule: Monday through Friday Method: Immunoassay Test Facility: Airport Way Regional Laboratory

Tacrolimus Level Tacrolimus (FK506)

Specimen Stability: Room Temperature: 72 hours Refrigerated: 1 week Frozen: 6 months

Processing: Do Not Centrifuge Transport: Transport to AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: Yellow ACD Solution B Volume: 7mL wb Alternative Collection: Yellow ACD Solution A Absolute Minimum Volume: 5mL wb Must reach testing site within 96 hours of collection. Draw Monday-Thursday only. Do not draw the day before a holiday.

TAT: 10 Days Test Schedule: Monday, Wednesday, and Friday Test Method: Heat Inactivation-Fluorometric-Manual Test Facility: Mayo Medical Laboratories

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200 First Street SW Rochester, MN 55905

Samples identified within the affected, carrier, or ambiguous enzyme ranges are reflexed to Tay-Sachs Disease, Mutation Analysis, HEXA at an additional charge Clinical Significance Diagnosing Tay-Sachs disease and carriers of Tay-Sachs, Sandhoff disease, and carriers of Sandhoff disease. Preferred test for determining carrier status. Tay-Sachs Carrier Screen Specimen Stability: Room temperature: 4 Days Refrigerated: 4 Days Frozen: Unacceptable TC# 82943 Send Direct to Mayo Medical Laboratories Must reach testing facility within 96 hours of draw

Processing: Do Not Centrifuge Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL whole blood

Test Schedule: MON-FRI with completed TPMG Molecular requisition Test Facility: Kaiser Regional Molecular Genetics Lab 5755 Cottle Rd. Bldg #6, San Jose, CA 95123 (408)972-3308.

Tay-Sachs Disease, Patient History: The answers to 4 questions must be supplied; Diagnostic or Carrier testing? Family history?, Ethnicity?, Pregnancy?. This info should be in tracking from EPIC/HC. Send info DNA Analysis with specimen.

Specimen Stability: Room Temp OK Refrigerate if held overnight Send Specimen with Kaiser Molecular Request Form

Processing: Do not centrifuge. Transport: Refrigerate if held.

Preferred Collection: RED 10 Alternative Collection: GRN Li Hep 4 and GRN Na Hep 4 Volume: 1.5mL s Testosterone Level AM Minimum Volume: 1.0 mL s/p

TAT: 24 hours Testing Schedule: Monday-Saturday

Note: Must be collected in the morning before 0900 (9:00AM).

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Processing: Centrifuge and aliquot. Refrigerate if held, up to 48 hrs. Freeze if testing is greater than 48 hrs.

Preferred Collection/Volume: RED 10 Volume: 1.5mL s Alternative Collection: GRN Li Hep 4 GRN Na Hep 4 Absolute Minimum Volume: 1.0 mL s/p

Testosterone Level TAT: 24 Hours Total Test Schedule: Mon - Sat Method: Immunoassay Test Facility: AWL

Processing: Centrifuge and aliquot. Refrigerate if held, up to 48 hrs. Freeze if testing is greater than 48 hrs.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 4 Days Test Schedule: Mon-Fri Morning Method: Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS) • Tracer Equilibrium Dialysis • Calculation Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Testosterone, Free and Total Clinical Data: Helpful in assessing testicular function in prepubescent hypogonadal males and in managing hirsutism, virilization in females.

Specimen Stability: Room temperature: 7 Days Refrigerated: 7 Days Frozen: 2 Years Reject Criteria: Serum Separator Tube (SST ®) Test Code: CHANTILLY TC 36170

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Next Day Test Schedule: Monday, Wednesday, Friday Afternoon Method: Enzyme immunoassay Test Facility: Quest Diagnostics Nichols Institute Tetanus Antibody 14225 Newbrook Drive Chantilly, VA 20153

Specimen Stability: Room temperature: 4 Days Refrigerated: 7 Days Frozen -20° C or lower: 30 Days Frozen -70° C or lower: Indefinitely Test Code: CHANTILLY TC 53952

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Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: AP99 Random Urine/ 50 mL u Alternative Collection: / 10 mL u

TAT: 24 Hours Test Schedule: Mon - Fri THC Quantitative Method: GC/MS Test Facility: AWL

To order a drug screen see panels CAN, DSA, DSB, and DSC. This panel is a quantitative THC/Creatinine ratio confirmation for positive cannabinoid screens

Preferred Collection: DkGRN Li Hep Alternative Collection: RED 10 DkGRN Na Hep Volume: 0.5 mL plasma/serum (Absolute Minimum Volume: 0.2 mL plasma/serum) NOTE: NO Gel Barrier tubes

Theophylline Level TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Method: Colorimeteric Rate Test Facility: Sunnyside Medical Center and Westside Medical Center

Note: Preferred draw time: Trough- immediately before next dose

Processing: Centrifuge. Aliquot. Refrigerate.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume:3.0 mL Serum

TAT: 24 hour Test Schedule: 7 days/week Method: Colorimetry Test Facility: Legacy Metrolab, 1225 NE Second Ave, Portland, OR 97232

Thiocyanate Level Rejection Criteria: Hemolysis LEGACY CS: 503-413-1234. NO TEST CODE

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Treat all Thiocyanate specimens as STAT. Cab directly to Legacy Central Lab with completed Legacy Requisition (available from Client Services) Complete Specimen Transport Form and transport to AWL in blue tote. Transport: Transport on ice.

Collection Instructions Preferred Collection/Volume: 1 LAV EDTA (Whole Blood) Volume: 4.0 mL WB Specimen Handling

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Processing: Do not centrifuge. Transport: To AWL in blue tote. Refrigerate if held.

TAT: 3 days Test Schedule: Monday-Friday Test Method: Testing Location: Prometheus Laboratories, Inc. 9410 Carroll Park Drive Thiopurine Metabolite San Diego, CA 92121, Level 1-888-423-5227

Specimen Stability Room Temperature: 24 hours Refrigerated: 7 Days Frozen: Do Not Freeze Note drug and dosage information on the Prometheus requisition TC # 3200

Preferred Collection/Volume: LAV EDTA Volume: 1.0 mL Plasma Alternative Collection: RED 10 Volume: 1mL serum

TAT: 4 Days Test Schedule: Monday-Friday, Sunday a.m.; Method: Gas Chromatography Test Facility: National Medical Services 3701 Welsh Rd Willow Grove, PA 19090

Clinical Data: Thioridazine is a phenothiazine for treating psychosis. Monitoring its level is Thioridazine important to optimize therapy, to avoid toxicity, and to assure compliance.

Specimen Stability: Room Temperature: 8 days Refrigerated: 8 days Frozen: 1 year Test Code: Quest TC 087

Processing: Centrifuge and aliquot Plasma/Serum into referred tests aliquot tube Note on tube if serum or plasma. Transport: Refrigerate if held.

Preferred Collection/Volume: Throat Swab: Liquid Amies Transport Swab (E-Swab) Alternative Collection: Throat Swab: Liquid Stuart Transport media

TAT: 2 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Throat Culture Clinical Data: This culture screens for group A beta-hemolytic Streptococcus. If Neisseria gonorrhoeae requested, order GC culture. Corynebacterium diptheriae cultures - order as referred test. If R/O thrush (yeast) requested, order Fungus Culture, Other. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired collection/transport device

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Greater than 24 hours post collection Dry Swab

Processing: Room Temperature Transport: Room Temperature

Preferred Collection/Volume: Fill tube completely. Gently mix but do not shake Blue Cit Volume: 1.0 mL Plasma

TAT: Next Day Test Schedule: Monday-Friday Morning Method: Clot Detection Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Thrombin Time Clinical Data: Thrombin clotting time measures the rate of fibrin polymerzation. It detects the presence of a direct thrombin inhibitor such as hirudin. It is elevated when fibrin split products are elevated in dysfibrinogemias.

Specimen Stability: Room Temperature: Unacceptable Refrigerated: Unacceptable Frozen: 1 Month Test Code: CHANTILLY TC 2893

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Freeze. Transport: Transport frozen on ice.

Preferred Collection/Volume: RD10/ 2.0 mL s Alternative Collection: SSRED5/ 0.5 mL s

TAT: 4 Days Test Schedule: Tue, Fri Thyrogl Ab Method: Chemiluminescent Immunoassay Test Facility: AWL

Processing: Centrifuge, aliquot, refrigerate if held. Freeze after 48 hours

Preferred Collection/Volume: RED 10 Volume: 2.0 mL s Alternative Collection: RED SST Volume: 1.0 mL s

TAT: 4 Days Test Schedule: Tue, Fri Thyroglob Panel Method: Chemiluminescent Immunoassay Test Facility: AWL

Panel contains Ab Screen and Thyroglobulin Tumor Marker

Processing: Centrifuge and aliquot 1.0mL serum into two aliquot tubes. Refrigerate if held. Freeze after 48 hrs

Preferred Collection/Volume: Sterile Container/ 2.0 mL bf Alternative Collection: / 0.8 mL bf

TAT: 4 Days

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Test Schedule: Tue, Fri Method: Chemiluminescent Immunoassay Test Facility: AWL Thyroglobulin Cyst Fluid For cyst fluid and saline washes

Processing: Refrigerate if held. Freeze after 48 hrs

Preferred Collection/Volume: RED SST Volume: 1.5 mL s Alternative Collection: RED 10 GRN Li Hep PST Absolute Minimum Volume: 1.0 mL

TAT: 24 Hours Thyroid Stimulating Test Schedule: Mon - Sat Hormone Method: Chemiluminescent Immunoassay Test Facility: AWL

This panel is for monitoring patients on thyroid medication. See panel TSR for thyroid function screening. TSH is a duplicate if both a TSH and TSR are requested together

Processing: Centrifuge, Aliquot if RED 10. Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 3 Days Test Schedule: Monday-Saturday Morning Method: Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Graves' disease is a classic form of hyperthyroid disease, affecting approximately 0.4% of the population in the United States. It is caused by IgG immunoglobulins, Thyroid Stimulating collectively known as thyroid stimulating immunoglobulins (TSI). Patients who are candidates for Immunoglobulins antithyroid drug therapy may not respond to this treatment when TSI levels are markedly elevated. The determination of TSI can also assist in predicting hyperthyroidism in neonates, due to placental transmission of the immunoglobulins from a mother with hyperthyroidism.

Specimen Stability: Room Temperature: unacceptable Refrigerated: 21 days Frozen: 60 days Rejection Criterion: gross hemolysis, gross lipemia, or gross icteric specimens are unacceptable. Test Code: CHANTILLY TC 30551

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Tranport on ice.

Preferred Collection/Volume: RED 10 Volume: 1.0 mL s Alternative Collection: RED SST Absolute Minimum Volume: 0.5 mL s Thyroperoxidase Antibody TAT: 1 Week Test Schedule: Tue Method: Chemiluminescent Immunoassay Test Facility: AWL

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Other names for this test include Anti-TPO and Microsomal Antibody.

Processing: Centrifuge and aliquot. Transport: Rrefrigerate if held. Freeze after 48 hours

Preferred Collection/Volume: RED10 Volume: 1mL s Absolute Minimum Volume: 0.5mL s

TAT: 2 days Test Schedule: Tuesday and Friday Mornings Test Method: Radioreceptor Assay (RRA) Testing Location: Quest Diagnostics Nichols Institute 1 4225 Newbrook Drive Chantilly, VA 20153

Clinical Significance Measurement of TBII is used to diagnose and manage Graves' disease, neonatal hypothyroidism, and postpartum thyroid dysfunction. Thyrotropin Binding Alternative Names(s) Inhibitory Igs TBII

Transport Temperature Refrigerated Specimen Stability Room temperature: 48 hours Refrigerated: 7 days Frozen: 30 days TC# 5738

Specimen Processing: Centrifuge and aliquot into Referred Tests aliquot tube Transportation: To AWL in blue tote

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Next Day Test Schedule: Monday-Friday Evening Method: Chemiluminescence Assay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Thyroxine Binding Chantilly, VA 20153 Globulin Clinical Data: Thyroxine-binding globulin (TBG) is used to differentiate quantitative TBG derangements from thyroid dysfunction. The measurement of TBG can also be used to establish the presence of TBG deficiency or excess, suggested by abnormal total T4 and T3 concentrations in the presence of normal free levels of these hormones.

Specimen Stability: Room Temperature: 7 days Refrigerated: 7 days Frozen: 30 days Test Code: CHANTILLY TC 4352

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Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 1.0 mL Plasma Alternative Collection: RED 10 No Gel Barrier Tubes Volume: 1 mL Serum

TAT: 5 Days Test Schedule: Monday, Wednesday, Friday Morning Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: NMS LABS 3701 Welsh Road Willow Grove, PA 19090 Tiagabine Level Patient Preparation: Draw Sample prior to dosing (trough).

NOTE: Sent to NMS via Quest. Specimen Stability: Room Temperature: 7 days Refrigerated: 7 days Frozen: 14 days (-20c) Test Code: Quest TC 11120

Processing: Promptly centrifuge and separate Plasma/Serum into a referrered Tests aliquot tube. Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: Sterile leakproof container/>1 mm Add a small amount of sterile saline to cover and keep moist. Transport to laboratory immediately. Alternative Collection: n/a

TAT: 1-3 Days Testing Schedule: Monday -Friday Method: Mutiplex Immunoassay Testing Location: Airportway Laboratory Note: If IgA levels are too low for measurement, TTG-IgG will be reflexed. Tissue Culture Clinical Data: Use this order for actual pieces of tissue/biopsies, not swabs of tissues. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Additive in sterile container (ie: formalin, RPMI) Greater than 24 hours post collection

Processing: Room Temperature Transport: Room Temperature

Preferred Collection: RED SST Volume: 1.0 mL serum Alternative Collection: RED 10 Minimum Volume: 0.2 mL serum

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TAT: 4 Days Test Schedule: Mon,Thurs Tissue Method: Immunoassay Transglutaminase Test Facility: AWL Antibody IgA Processing: Centrifuge, aliquot (if collected in a RED 10), refrigerate.

Preferred Collection: RED SST Volume: 1.0 mL serum Alternative Collection: RED 10 Minimum Volume: 0.2 mL serum

T AT: 1-3 Days Testing Schedule: Monday -Friday Method: Mutiplex Immunoassay Tissue Transglutaminase Testing Location: Airportway Laboratory Antibody IgG Clinical Significance Tissue Transglutaminase Antibody, IgG, is useful in diagnosing gluten-sensitive enteropathies, such as Celiac Sprue Disease, and an associated skin condition, dermatitis herpetiformis in patients who are IgA-deficient. Alternative Names(s) Transglutaminase, Tissue Antibody IgG, TTG IgG

Processing: Centrifuge, aliquot (if collected in a RED 10), refrigerate.

Preferred Collection: DkGrn Li Hep Alternative Collection: RED 10 DkGrn Na Hep LAV EDTA - must be filled at least half full Volume: 0.5 mL plasma/serum (Absolute minimum volume: 0.2 mL plasma/serum) NOTE: NO gel barrier tubes Tobramycin Level TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Method: Immunoassay Test Facility: Sunnyside Medical Center and Westside Medical Center

Processing: Centrifuge and aliquot within 1 hour of collection. Refrigerate if held. Transport: Send specimen to appropriate Medical Center for testing Note: City Sprint STATs directly to appropriate Medical Center for testing

Preferred Collection: DkGrn Li Hep Alternative Collection: RED 10 LAV EDTA - must be filled at least half full

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Volume: 0.5 mL plasma (Absolute Minimum Volume: 0.2 mL) Note: NO Gel Barrier tubes

TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Method: Immunoassay Test Facility: Sunnyside Medical Center and Westside Medical Center Tobramycin Level Peak Note: Preferred draw time: IV - Draw 30 min after end of 30 min infusion or 15 min after end of 45-60 min infusion. IM - Draw 60 min post injection

Processing: Centrifuge and aliquot within 1 hour of collection. Refrigerate if held. Transport: Send specimen to appropriate Medical Center for testing Note: City Sprint STATs directly to appropriate Medical Center for testing.

Preferred Collection: DkGrn Li Hep Alternative Collection: RED 10 LAV EDTA - must be filled at least half full Volume : 0.5 mL plasma/serum (Absolute Minimum Volume: 0.2 mL plasma/serum) Note: NO gel barrier tubes

TAT: STAT: 1 Hour Tobramycin Level Routine: 24 Hours Trough Test Schedule: Daily Method: Immunoassay Test Facility: Sunnyside Medical Center and Westside Medical Center

Note: Preferred draw time is trough, immediately before next dose.

Processing: Centrifuge and aliquot within 1 hour of collection. Refrigerate if held. Transport: Send specimens to appropriate Medical Center for testing. Note: City Sprint STATs directly to appropriate Medical Center for testing

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 2 to 4 days Test Schedule: 5 days a week, night Method: Flourescent Polarization Immunoassay Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130 Topiramate Level Clinical Data: Topiramate is an anticonvulsant used as an adjunctive treatment of partial-onset epilepsy. Therapeutic drug monitoring is useful to optimize dose and avoid toxicity.

Patient Preparation: Draw at peak (2-4 hours after dose) or trough (0.5-1 hour before dose) at steady state.

Specimen Stability: Room Temperature: 7 Days Refrigerated: 14 Days Frozen: 30 Days Rejection Criteria:

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Reject Hemolysis: Yes Reject Lipemia: Yes Reject Thaw/Other: Gel barrier tube Test Code: SJC TC 30965X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RD10/ 1.0 mL

TAT: TOR: 24 Hours Test Schedule: Mon - Fri Method: TOR: Multiplex flow Immunoassay Test Facility: AWL + Referred Test (Quest)

TORCH Neonatal Toxo Test includes Toxoplasmosis IgG(the TOR for AWL), and Rubella IgM/CMV IgM/Herpes IgM (the CH2 for AML). The 2 parts are aliquoted separately. If cord blood or from newborn less than 1 month old, collect mother's blood also and order separately.

Processing: Centrifuge and aliquot into plastic tubes labeled as follows: "TOR" for Dept SA at AWL and "CH2" for Dept RF for send out. TESTED AT: CH2 to Quest, Chantilly, VA TC # 12752

Preferred Collection/Volume: SSRED5/ 1.0 mL s Alternative Collection: RD10/ 1.0 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Toxoplasma Antibody Method: Multiplex flow Immunoassay IgG Test Facility: AWL

CSF is not an acceptable specimen for this test. A positive result automatically reflexes Toxo IgM Ab panel (a send out-TAM)for Kaiser patients

Processing: Centrifuge, aliquot if RD10. Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Next Day Test Schedule: Monday-Friday Morning Method: Enzyme Immunoassay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Toxoplasma Antibody IgM Clinical Data: Please note: This test should only be ordered on patients who have already had Toxoplasma IgG testing performed, and whose immune status is known. If acute infection is suspected, the specimens should be tested for the presence of both IgG and IgM antibodies. The result of any one Toxoplasma IgM test should not be used as the sole determinant of recent Toxoplasma infection.

Specimen Stability: Room Temperature: 4 Days Refrigerated: 7 Days Frozen: 30 Days Test Code: CHANTILLY TC 37207

Processing: Centrifuge and separate serum from cells as soon as possible. Avoid repeated freeze/thaw

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cycles. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Next Day Test Schedule: Monday-Saturday Afternoon Method: Fixed Rate Time Nephelometry Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Please note: CSF is not an acceptable sample type for this test. Transferrin Patient Preparation: Fasting for 12 hours is required.

Specimen Stability: Room Temperature: 3 Days Refrigerated: 7 Days Frozen: 90 Days (-20c) Test Code: CHANTILLY TC 891

Processing: Centrifuge and separate serum from cells as soon as possible. Avoid repeated freeze/thaw cycles. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 1.0 mL Plasma Alternative Collection: RED 10 Volume: 1mL Serum NO RED SST

TAT: 4 Days Test Schedule: Thursday Morning Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute Valencia 27027 Tourney Road Valencia, CA 91355-5386

Trazodone Level Clinical Data: Test is used for therapeutic drug monitoring of antidepressant therapy. Peak plasma levels occur approximately 1 hour after dosing when Trazodone is taken on an empty stomach or 2 hours after dosing when taken with food. Elimination of Trazodone is biphasic, consisting of an initial phase (half-life 3-6 hours) followed by a slower phase (half-life 5-9 hours).

Note: Test Performed at Quest Valencia via Quest Chantilly Specimen Stability: Room Temperature: 7 days Refrigerated: 14 days Frozen: 60 days (-20c) Test Code: QUEST TC 1312

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Same day Test Schedule: 1 day a week, a.m

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Method: ENZYME LINKED IMMUNOSORBENT IMMUNOASSAY Test Facility: Focus Diagnostics, Inc. 5785 Corporate Ave. Cypress, CA 90630

Specimen Stability: Room temperature: 7 Days Trichinella Antibody Refrigerated: 14 Days Frozen: 30 Days Test Code: SJC TC 65631P TO FOCUS TC 40990

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: Random Urine in AP99 Volume 4.0mL Urine

TAT: 3 Days Test Schedule: Monday-Saturday Afternoon Method: Colorimetry (C) Spectrophotometry (SP) Test Facility: NMS Labs 3701 Welsh Road Willow Grove, PA 19090 Trichloroacetic Acid, Urine Clinical Data: Test is for exposure monitoring in urine. Specimen Stability: Room Temperature: Undetermined Refrigerated: Undetermined Frozen (-20 °C): Undetermined Test Code: CHANTILLY TC 5036X TO NMS TC 4627U

Processing: Collect sample at end of shift at end of work week. Transport:

Preferred Collections: Swab in conical tube with discharge/secretion, ensure a few drops of saline is added or Random urine (AP99) Minimum Volume: Random urine 1mL

TAT: 1 hour Test Schedule: Daily Trichomonas Wet Mount Testing Location: Testing is available at Medical Office Buildings and Medical Centers.

For best results, testing should occur within one hour of collection.

This test is for Male patients only.

Processing: Transport specimen immediately at room temperature to the testing lab.

Preferred Collection/Volume: AP99 Random Urine/ 50 mL U Alternative Collection: / 10 mL U

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TAT: 24 Hours Test Schedule: Mon - Sat Method: GC/MS Tricyclic Urine Test Facility: AWL Confirmatory To order a drug screen, see panel DSS or DSC

Processing: Refrigerate if held overnight

Preferred Collection/Volume: LIGREEN4/ 4.0mL bf Alternative Collection: RD10/ 1.0mL bf

TAT: 24 Hours Test Schedule: Daily Triglyceride Body Fluid Method: Enzymatic Colorimetric Test Facility: AWL

Use separate LIS requests if both blood and body fluids are ordered

Processing: Refrigerate if held

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p/s Alternative Collection: RED SST or serum acceptable LAV EDTA Absolute Minimum Volume: 0.2mL p/s

TAT: 24 Hours Triglyceride Fasting Test Schedule: Daily Method: Enzymatic Colorimetric Test Facility: AWL

Patient Preparation: Patient must be fasting for 10 hours.

Processing: Cent., Refrigerate if held.

Preferred Collection/Volume: GNS5/ 0.5mL p Alternative Collection: SSRED5, RD10, LV5, LIGREEN4/ 0.2mL p/s

TAT: 24 Hours Test Schedule: Daily Triglycerides Method: Enzymatic Colorimetric Test Facility: AWL

Use this test code when pt fasting less than 10 hours

Processing: Cent., Refrigerate if held

Preferred Collection/Volume: LAV EDTA Volume: 5.0 mL whole blood

TAT: 4 Days Test Schedule: Monday-Friday, Sunday Afternoon Method: Gas Chromatography (GC) Test Facility: NMS LABS 3701 Welsh Road Willow Grove, PA 19091

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Clinical Data: Test is for Therapeutic Drug Monitoring

NOTE: Sent to NMS via Quest. Specimen Stability: Room Temperature: 7 day(s) Trihexyphenidyl Level Refrigerated: 14 day(s) Frozen (-20 °C): 12 month(s) Test Code: Quest TC 44231

Processing: Do Not Centrifuge Transport: Refrigerate if held.

Preferred Collection/Volume: 2 mL Serum or Plasma

TAT: 3 days Test Schedule: Monday Method: Gas Chromatography (GC) Test Facility: NMS Labs 3701 Welsh Road Willow Grove, PA 19090

Clinical Data: Therapeutic Drug Monitoring Trimethadione Specimen Stability: Room Temperature: 7 day(s) Refrigerated: 14 day(s) Frozen (-20 °C): 12 month(s) Rejection Criteria: Polymer gel separation tube (SST or PST) Test Code: NMS TC 4700SP

Processing: Centrifuge and aliquot into a Referred Test aliquot tube.Note if serum or plasma on specimen. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume:3.0 mL Serum

TAT: 4 Days Test Schedule: Sunday-Thursday, Night Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130 Trimipramine Patient Preparation: Optimum time to collect sample: 10-14 hours post oral dose.

Specimen Stability: Room Temperature: 4 Hours Refrigerated: Unacceptable Frozen: 30 Days Test Code: CHANTILLY TC 37123 TO SJC TC 40832P

Processing: Centrifuge ASAP after clotting, aliquot into Referred Tests aliquot tube and freeze. Transport: Transport frozen on ice

Preferred Collection/Volume: LAV EDTA Volume: 3mL p

TAT: Report available: 17-21 days after setup

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Test Schedule: Monday-Sunday AM Test Method: HIV Co-Receptor Tropism Assay Test Location: Monogram Biosciences 345 Oyster Point Boulevard S. San Francisco, CA 94080

Patient Preparation For best results, viral loads should be confirmed within the two weeks prior to submission for analysis at Monogram Biosciences. Indicate on the test request form the patient's most recent viral load and viral load collection date. Clinical Significance The Trofile™ Co-Receptor Tropism Assay is intended for use only with patients having viral loads ≥1000 copies/mL.

Trofile(TM) CO- Indicate on the test request form the patient's most recent viral load and viral load collection Receptor T date. Transport Temperature: Frozen Specimen Stability Room temperature: Unacceptable Refrigerated: Unacceptable Frozen: 14 days Reject Criteria Received room temperature • Received refrigerated TC# 18578

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Freeze Transportation: Transport frozen on ice

Preferred Collection: GRN Li Hep PST Alternative Collection: RED SS RED10 EDTA Volume: 0.5mL plasma/serum (Absolute Minimum Volume: 0.3mL plasma/serum)

Troponin-I TAT: 1 Hour Test Schedule: Daily Method: Immunoassay Test Facility: Sunnyside Medical Center and Westside Medical Center Add On Testing Stability: 7 days if refrigerated @ 2-8 degrees celsius

Processing: Centrifuge. Aliquot if no gel barrier Transportation: Transport to testing location on ice.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 3 days Test Schedule: 2 days a week a.m Method: Radioimmunoassay

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Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Trypsin (or trypsinogen) is considered a specific indicator of pancreatic damage. Increased values over the determined normal range may indicate inflammatory pancreatic condition.

Specimen Stability: Room temperature: 7 Days Trypsinogen (Trypsin) Refrigerated: 7 days Frozen: 28 Days Reject Criteria: Hemolysis; Lipemia; Samples containing radioactive compounds such as those from patients who have undergone in-vivo radioisotope testing. Test Code: SJC TC 30329X

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum Alternative Collection: LAV EDTA Volume: 1.0 mL Plasma

TAT: 1 day Test Schedule: 2 days a week a.m Method: Fluorenzyme Immunoassay Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Tryptase Clinical Data: Tryptase concentrations are increased with immediate hypersensitivity (anaphylaxis), acute allergen challenge, and mastocytosis.

Specimen Stability: Room temperature: 48 Hours Refrigerated: 5 Days Frozen: 30 Days Reject Criteria Serum Separator Tube Test Code: SJC TC 34484X

Processing: Centrifuge ASAP and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: Green NA HEP Volume: 2 mL Plasma

TAT: 6 days Test Schedule: 3 days a week, a.m Method: Liquid Chromatography Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute Tryptophan 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Amino Acid analysis is necessary for the diagnosis of a variety of inborn errors of metabolosm. These include, but are not limited to, phenylketonuria, tyrosinemia, citrullinemia, non- ketotic hyperglycinemia, maple syrup urine disease, and homocystinuria. The assay is also key for the continued monitoring of treatment plans for these disorders and useful for assessing nutritional status of patients. Our methodology is highly accurate at very low levels as well as at elevated levels.

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Patient Preparation: Collect plasma specimens after an overnight fast (or at least 4 hours after a meal). Non-fasting samples are acceptable for pediatric patients.

Specimen Stability: Room temperature: Unstable; Refrigerated: 7 Days; Frozen: 30 Days; Reject Criteria: Hemolysis; Samples that are received at ambient temperature Test Code: QUEST SAN JUAN TC 959

Processing: Centrifuge and aliquot info Referred Tests aliquot tube as soon as possible after collection. Freeze. Transport: Transport frozen on ice.

Preferred Collection/Volume: RED SST Volume: 1.5 mL s Alternative Collection: RED 10 GRN Li Hep PST Absolute Minimum Volume: 1.0 mL

TAT: 24 Hours Test Schedule: Mon - Sat TSH w Reflex to FT4 Method: Chemiluminescent Immunoassay Test Facility: AWL

Screening will test for thyroid function. TSH reflex's Free T4 (panel T4F) if the TSH is abnormal. See panel TSH for patients on thyroid medication. TSH is a duplicate if both the TSH and TSR are requested together

Processing: Centrifuge, Aliquot if RED 10 . Refrigerate if held.

Preferred Collection/Volume: Pink EDTA Volume: 5.0 mL Type and Screen Alternative Collection: LAV EDTA Prenatal RED 10 (do not centrifuge) Absolute Minimum Volume: 1.0 mL

Preferred Collection/Volume: urine submitted in sterile collection container Volume: 10 mL u

TAT: 24 Hours Test Schedule: Daily Method: Immunoassay U Fentanyl Scr Test Facility: Airport Way Regional Laboratory

Positive screen results for fentanyl are not confirmed by additional testing

Processing: Keep specimen cold until transport Transportation: Transport to Airport Way Laboratory

Preferred Collection/Volume: At least 10 mLs of random "clean catch" midstream urine in AP99 or UVAC (no preservative).

TAT: 1 day Test Schedule: Daily Method: D ipstick Test Facility: AWL, SMC, WMC, Medical Office labs

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Patient Preparation: Provide patient with a pre-labeled container and instructions for collecting "clean catch" specimen.

Test within 2 hours after collection or refrigerate for up to 24 hours before testing. At least 10 ml of clean catch, midstream urine is the preferred specimen UA Glucose Processing: Refrigerate if not tested within 2 hours. Must be tested within 24 hours. Transport: Transport refrigerated to testing facility. Rejection Criteria: Urine not refrigerated within 2 hours or urine greater than 24 hours post-collection.

Preferred Collection/Volume: 24 hr urine (no preservative)/ 4.0mL u Alternative Collection: 12x75 aliq./ 1.0mL u

TAT: 24 Hours Test Schedule: Daily Method: Photometric Rate Urea Nitrogen 24 Hour Test Facility: AWL Urine Refrigerate during collection. Preservatives are not acceptable. See panel RUN for random specimen.

Processing: Record collection interval hrs. Measure and record total vol. in ARE. Aliquot to 12x75 tube. Refrigerate

Preferred Collection/Volume: LIGREEN4/ 4.0mL bf Alternative Collection: RD10/ 1.0mL bf

TAT: STAT: 1 Hour Urea Nitrogen Body Routine: 24 Hours Test Schedule: Daily Fluid Method: Photometric Rate Test Facility: AWL, SMC

Processing: Refrigerate if held

Preferred Collection/Volume: AP99 with 12x75 aliquot/ 4.0mL u Alternative Collection: / 1.0mL u

TAT: 24 Hours Test Schedule: Daily Urea Nitrogen Urine Method: Photometric Rate Test Facility: AWL, SMC

Processing: Aliquot to 12x75 tube. Refrigerate

Preferred Collection/Volume: GRN Li Hep PST Volume: 0.5mL p/s Alternative Collection: RED SST RED 10 Uric Acid Absolute Minimum Volume: 0.2mL p/s TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Method: Photometric, Uricase Reaction

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Test Facility: AWL, SMC, INT, BVT

Processing: Centrifuge. Refrigerate if held

Preferred Collection/Volume: 24 hr Urine/ 4.0 mL u Alternative Collection: 12x75 aliq./ 1.0mL u

TAT: 24 Hours Test Schedule: Daily Uric Acid 24 Hour Method: Photometric, Uricase Reaction Urine Test Facility: AWL

Refrigerate during collection without preservatives. See panel RUA for random specimen

Processing: Record collection interval hrs. Measure and record total vol. in ARE in LIS. Mix well then aliquot to 12x75 tube. Adjust aliquot to pH 8-9 with 1N NaOH.

Preferred Collection/Volume: AP99 with 12x75 aliq./ 4.0mL u Alternative Collection: / 1.0mL u

TAT: 24 Hours Test Schedule: Daily Uric Acid Urine Method: Photometric, Uricase Reaction Test Facility: AWL

Processing: Aliquot to 12x75 tube. Adjust aliquot to pH 8-9 with 1N NaOH.

Preferred Collection/Volume: At least 10 mLs of random "clean catch" midstream urine in AP99 or UVAC (no preservative)

TAT: 24 hours Test Schedule: Daily Method: UA dipstick Test Facility: AWL, SMC, WMC, Medical Office labs

Patient Preparation: Provide patient with a pre-labeled contianer and instructions Urinalysis Dipstick Only for collecting a "clean catch" specimen.

Processing: Refrigerate if not tested within 2 hours. Must be tested within 24 hours. Transport: Transport refrigerated to testing facility. Rejection Criteria: Specimen greater than 24 hours old or unrefrigerated greater than two hours.

Preferred Collection/Volume: At least 10 mLs of random "clean catch" midstream urine in AP99 or UVAC (no preservative).

TAT: 24 hours Test Schedule: Daily Urinalysis Microscopic Method: Microscopy manual or automated Test Facility: AWL, SMC, WMC, Medical Office labs

Patient Preparation: Provide patient with a pre-labeled contianer and instructions for collecting "clean catch" specimen.

Can be ordered separately per clinician preference.

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Processing: Refrigerate if not tested within 2 hours. Must be tested within 24 hours. Transport: Transport refrigerated to testing facility. Rejection Criteria: Urine not refrigerated within 2 hours or urine greater than 24 hours post-collection.

Preferred Collection/Volume: At least 10 mLs of random "clean catch" midstream urine in AP99 or UVAC (no preservative).

TAT: 24 hours Test Schedule: Daily Method: UA dipstick Test Facility: AWL, SMC, WMC, Medical Office labs

Patient Preparation: Provide patient with a pre-labeled contianer and instructions Urinalysis Reflex Micro for collecting "clean catch" specimen. and Culture Urine microscopic and/or culture will be automatically reflexed per lab policy.

Processing: Refrigerate if not tested within 2 hours. Must be tested within 24 hours. Transport: Transport refrigerated to testing facility. Rejection Criteria: Urine not refrigerated within 2 hours or urine greater than 24 hours post-collection.

Preferred Collection/Volume: At least 10 mLs of random "clean catch" midstream urine in AP99 or UVAC (no preservative).

TAT: 24 hours Test Schedule: Daily Method: UA dipstick, microscopy (if indicated) Test Facility: AWL, SMC, WMC, Medical Office labs

Patient Preparation: Provide patient with a pre-labeled contianer and instructions for collecting "clean catch" specimen. Urinalysis w/o Culture Includes culture if indicated, no microscopic performed regardless of dipstick results.

Processing: Refrigerate if not tested within 2 hours. Must be tested within 24 hours. Transport: Transport refrigerated to testing facility. Rejection Criteria: Urine not refrigerated within 2 hours or urine greater than 24 hours post-collection

Preferred Collection/Volume: Urine submitted in sterile container Volume: >1.0mL u Submit reflexed urine cultures in sterile AP99 container

TAT: 2 - 3 Days Test Schedule: Daily Urine Culture Method: Culture Test Facility: AWL

Clinical Data: Please indicate source on specimen

Please indicate source on specimen.

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Rejection Criteria: Sample submitted >24 hours post collection Specimen collected in a non-sterile container Specimen submitted <1mL

Processing: Keep sample at refrigerated temperature until transport Transport: Transport to Airport Way Laboratory on ice

Preferred Collection/Volume: At least 2 mLs clean "catch midstream" urine in AP99 or UVAC (no preservative).

TAT: 24 hours Test Schedule: Daily Method: UA dipstick Test Facility: AWL, SMC, WMC, Medical Office labs

Urobilinogen Urine Patient Preparation: Provide patient with pre-labeld container. Clean catch not necessary if only test ordered.

Processing: Refrigerate if not tested within 2 hours. Protect from light. Test within 24 hours. Transport: Transport refrigerated to testing facility. Rejection Criteria: Urine not refrigerated within 2 hours or urine greater than 24 hours post-collection.

Preferred Collection/Volume: GRN Li Hep 4 NO Gel Barrier Tubes Volume: 0.5 mL p/s Alternative Collection: RED 10 LAV EDTA GRN Na Hep 4 Absolute Minimum Volume: 0.2 mL p/s

Valproic Acid Level TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Method: Enzyme Immunoassay Test Facility: AWL, SMC

Processing: Centrifuge, aliquot, refrigerate

Preferred Collection: LAV EDTA Alternative Collection: RED 10 Volume: 1.0 mL plasma (Absolute Minimum Volume: 0.5 mL plasma or serum) Note: NO gel barrier tubes

TAT: 24 Hours Test Schedule: Daily Vancomycin Level Method: Immunoassay Test Facility: Sunnyside Medical Center and Westside Medical Center Add on Stability: 3 days if refrigerated

Processing: Centrifuge and aliquot within 4 hours of collection Transportation: Transport to testing location on ice.

Preferred Collection: LAV EDTA Alternative Collection: RED10

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Volume: 1.0mL plasma (Absolute Minimum Volume: 0.5 mL plasma or serum) Note: NO gel barrier tubes

TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Method: Immunoassay Vancomycin Level Peak Test Facility: Sunnyside Medical Center and Westside Medical Center

Note: Preferred draw time: 30-60 mins. after end of infusion. Record last dose/next dose info.

Processing: Centrifuge and aliquot within 4 hours of collection Transportation: Transport to testing location on ice.

Preferred Collection: LAV EDTA Alternative Collection: RED10 Volume: 1.0mL plasma (Absolute Minimum Volume: 0.5 mL plasma or serum) Note: NO Gel barrier

TAT: STAT: 1 Hour Routine: 24 Hours Test Schedule: Daily Vancomycin Level Method: Immunoassay Trough Test Facility: Sunnyside Medical Center and Westside Medical Center

Note: Preferred draw time: immediately before next dose. Record last dose/ next dose info.

Processing: Centrifuge and aliquot within 4 hours of collection Transportation: Transport to testing location on ice.

Preferred Collection/Volume: SSRED5/ 1.0 mL s Alternative Collection: RD10/ 1.0 mL s

TAT: 24 Hours Test Schedule: Mon - Fri Varicella Zoster Method: Multiplex flow Immunoassay Antibody IgG Test Facility: AWL

Test for IgG antibody detection only. See panel Varicella IgM (VAG) for acute case evaluation for prenatal exposure when antibody status is unknown

Processing: Centrifuge and aliquot if RD10. Refrigerate if held

Preferred Collection/Volume: RED 10 or RED SST Volume: 1 mL Serum

TAT: Next day Test Schedule: Mon-Fri Morning Varicella Zoster Method: Enzyme Immunoassay Antibody IgM Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Varicella-Zoster Virus (VZV) causes chicken pox and when reactivated, potentially decades later, causes shingles. Twenty percent of adults will develop shingles, a rash or blister of the skin that may cause severe pain.

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Specimen Stability: Room temperature: 4 days Refrigerated: 7 days Frozen: 30 days Test Code: Quest TC 23072

Processing: Centrifuge and aliquot serum into Referred Tests aliquot tube Transport: Refrigerate if held.

Specimen Collection: Vescular lesion swab, throat swab, or tissue biopsy in a Viral Transport Media. Tracheal aspirate, Bronchial lavage/wash, Sputum, Tissue biopsy or fresh (unfixed) tissue in a sterile screw cap container. Eye swab in a Viral Transport Media Volume: 3 mL Minimum Volume: 1mL Note: To maintain optimum viability, place swab or fluid into VCM (equal volumes of fluid and VCM) or equivalent and transport the specimen to the laboratory as soon as possible. Best recovery is obtained when the specimens are refrigerated at 2-8° C or kept on wet ice following collection and while in transit. If there will be a long delay before processing, specimens in VCM or equivalent should be frozen at -70° C or colder and transported on dry ice. Storage or transport at -20° C is not acceptable. Raw (unpreserved) samples should only be refrigerated and not frozen.

TAT: Report available in 3-5 days Testing Location: Focus Diagnostics, Inc. (via Quest Diagnostics) Varicella zoster culture 5785 Corporate Ave. Cypress, CA 90630 Test Code: 2691 Specimen Stability: Room Temperature: Unacceptable Refrigerated: 72 hours Frozen: -20C Unacceptable -70C 30 days Rejection Criteria: Wooden shaft and calcium alginate swabs , Raw (unpreserved) stool specimens, Dry swabs, Transwabs® or gel-based transport medium, DNA probe transports, Tissues in formalin or other fixatives http://www.questdiagnostics.com/testcenter/TestCenterHome.action

Note: CSF specimens are not acceptable for a Varicella Zoster Culture, PCR is recommended.

Processing: Refrigerate specimen Transportation: Send specimens on ice to Airport Way Lab Referred Test Department

Preferred Collection/Volume: LAV EDTA

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Volume: 3.0 mL Plasma

TAT: 5 days Test Schedule: 2 days a week p.m. Method: Extraction • Radioimmunoassay Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: VIP is a . VIP-secreting tumors, most commonly found in the tail Vasoactive Intestinal of the pancreas, can cause secretory diarrhea. In children, the tumors are ganglioneuromas or Polypeptide ganglioneuroblastomas and commonly occur in the adrenal glands. Specimen Stability: Room temperature: 72 Hours Refrigerated: 7 Days Frozen: 28 Days Reject Criteria: Lipemia; Room temperature Test Code: SJC 920X

Processing: Centrifuge immediately and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: CSF in a Sterile Container, leak-proof Volume:1 mL

TAT: Next Day Test Schedule: Monday-Saturday Morning, Night Method: Slide Micro Flocculation Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 VDRL Screen CSF Specimen Stability: Room Temperature: 4 Days Refrigerated: 14 Days Frozen: 30 Days Test Code: CHANTILLY TC 7244

Processing: Do not centrifuge. Transport: Refrigerate if held.

Preferred Collection/Volume: LAV EDTA Volume: 1.0-3 mL Plasma Alternative Collection: RED 10 Volume 1mL Serum

Test Facility: OHSU Mail code BTE355 3181 S.W. Sam Jackson Park Road Very Long Chain Fatty Portland, OR 97239 Acids Clinical Data: For diagnosis of adrenoleukodystrophy, Zellweger syndrome and other peroxisome assembly disorders. If both phytanic and very long chain fatty acids are to be done, one need only draw a single 1-3 mL blood specimen.

Specimen Stability: Critical Frozen Test Code: OHSU TC 067-6334

Processing: Centrifuge and aliquot into Referred Tests aliquot tube Note if serum or plasma on specimen. Freeze

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Transport: Transport frozen on ice.

Preferred Collection/Volume: Fresh Stool Specimen: Place a portion (> 2 grams) of the stool specimen into the Enteric Pathogen Transport Vial within 2 hours of collection. Alternative Collection: Rectal Swab: Liquid Amies Transport swab (E-Swab)

TAT: 3 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: n/a Patient History: n/a

Vibrio Culture Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Enteric Pathogen vial > 72 hours post collection Fresh stool > 2 hours post collection Rectal Swab > 48 hours post collection Expired collection/transport device Specimen contaminated with urine Specimen received in diaper

Processing: Refrigerate Transport: Send on Ice

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: Next Day Test Schedule: Monday-Friday Evening Method: Kinematic Viscometer Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Viscosity Clinical Data: High serum viscosity may be most commonly observed in patients with Waldenström's macroglobulinemia and multiple myeloma. Patients with high viscosity may have capillary occlusion, stasis hypoxia, and acidosis

Specimen Stability: Room temperature: 7 Days Refrigerated: 14 days Frozen: 6 Months Test Code: CHANTILLY TC 918

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: 3 Days Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive

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Chantilly, VA 20153

Clinical Data: Vitamin A is critical for vision, growth, and many cell functions. High concentrations of Vitamin A are seen with renal failure, but this is not associated with toxicity and excessive ingestion. High concentrations are associated with bone fractures. Low concentrations of Vitamin A are consistent with fat malabsorption and are rarely due to inadequate diet.

Patient Preparation: Overnight fasting is preferred.

Vitamin A Level Specimen Stability: Frozen: 1 Year Rejection criteria: Received unfrozen or specimen was not protected from light. Test Code: CHANTILLY TC 921

Processing: Centrifuge serum specimen within 1 hour of collection. Transfer serum to plastic, amber vial. If amber vial is not available, wrap tube in aluminum foil to protect from light. Transport: Frozen to Reffered Tests

Preferred Collection/Volume: RED 10 Volume: 1.5mL s Alternative Collection: GRN Li Hep GRN Na Hep Volume: 1.0 mL plasma

Vitamin B 12 and Folate TAT: 24 Hours Test Schedule: Mon-Sat Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, pour, refrigerate. Freeze after 48 hrs

Preferred Collection: LAV EDTA Alternative Collection: Grn NA Hep or Dk Grn Li Hep Minimum Volume: 1.1 ml whole blood

TAT: Report available within 3 days from specimen receipt at testing location. Test Schedule: Monday, Wednesday, Friday Morning Method: High Performance Liquid Chromatography Testing Location: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153 Test Code: 5042 Specimen Stability: Vitamin B1 Room Temperature: 4 hours Refrigerated: 24 hours Frozen: 30 days Rejection Criteria: Received thawed Received room temperature Received Refrigerated Note: Specimens received frozen, but not protected from light are acceptable. Wrap the specimen in foil before sending to resting location. No disclaimer is required.

Clinical Significance

Vitamin B 1 is required for branched-chain amino acid and carbohydrate

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metabolism. Vitamin B 1 deficiency is most often due to alcoholism or chronic illness. In the early stage, patients with vitamin B 1 deficiency exhibit anorexia, irritability, apathy, and generalized weakness. Prolonged deficiency causes beriberi. Alternative Names(s)

B 1 ,Thiamine

Processing: Wrap specimen in foil to protect it from light. Then feeze specimen immediately. Transport: Protect specimen from light and send frozen specimen in a thermos wrapped in t-cells. Send to Airport Way laboratory Referred Tests department in a Blue tote.

Preferred Collection/Volume: RED 10 Volume: 1.5 mL Alternative Collection: GRN Li Hep 4 GRN Na Hep 4 Absolute Minimum Volume: 1.0 mL

Vitamin B12 Level TAT: 24 Hours Test Schedule: Mon-Sat Method: Immunoassay Test Facility: AWL

Processing: Centrifuge, Aliquot, and Refrigerate. Freeze after 48 hrs.

Preferred Collection/Volume: Wrap in foil to protect from light while collecting sample. LAV EDTA Volume: 1.0 mL Plasma

TAT: 4-5 Days Test Schedule: 5 Days a week. Method: Liquid Chromatography/Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Test is used to determine vitamin B6 deficiency or overdosage, for monitoring treatment, and to evaluate wellness and health.

Patient Preparation: Patient must fast overnight. Patient must be restricted from alcohol and Vitamin B6 Level vitamins for at least 24 hours before a sample collection.

Specimen Stability: Room Temperature: 6 Hours Refrigerated (cold packs): 12 Hours Frozen -20 degrees C: 6 Days Frozen -70 degrees C: 42 Days Rejection Criteria: Specimens not protected from light; Shipped/stored refrigerated or at room temperatures; Specimens left at room temperature over 6 hours; Collected in tubes other than lavender-top tubes; Lipemic or hemolyzed specimens; Improperly labeled tubes Test Code: SJC TC 926

Processing: Centrifuge and aliquot into dark brown aliquot tubes to protect from light. Freeze Transport: Transport frozen on ice

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes

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Volume: 2.0 mL Serum

TAT: 3 days Test Schedule: 5 days a week, P.M. Method: Capillary Electrophoresis Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Vitamin C is an antioxidant involved in connective tissue metabolism, drug- metabolizing systems, and mixed-function oxidase systems to list a few. Vitamin C deficiency causes scurvy; manifestations include impaired formation of mature connective tissue, bleeding into the skin, weakness, fatigue, and depression.

Patient Preparation: Overnight fasting is preferred. Vitamin C Level Patient should refrain from taking vitamin C supplements or fruits 24 hours prior to sample collection.

Specimen Stability: Serum or Plasma: Room temperature: Unacceptable Refrigerated: Unacceptable Frozen: See Processing under the Specimen Hanlding section -70 Degrees: 28 Days Reject Thaw/Other: Thawed Serum, Thawed Plasma, Received room temperature, Received refrigerated Test Code: SJC TC 929X

Processing: Centrifuge and aliquot into dark brown aliquot tubes to protect from light. Freeze Transport: Transport frozen on ice

Preferred Collection/Volume: RED SST Volume: 1.0 mL s Alternative Collection: RED 10 Absolute Minimum Volume: 0.5 mL s

TAT: 24 Hours Vitamin D 25 Hydroxy Test Schedule: Monday-Friday Level Method: Immunoassay Test Facility: Airport Way Regional Laboratory

Processing: Centrifuge Red SST. Centrifuge and aliquot if specimen is RED 10 Transportation: To AWL in blue tote. Refrigerate if held.

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum

TAT: 3 Days Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute Vitamin E Level 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Deficiency of vitamin E may cause extensive neuropathy in young children and, in addition, is suspect as a possible cause of motor and sensory neuropathy in older children and in adults. One likely cause of vitamin E deficiency is intestinal malabsorption, resulting from bowel disease, pancreatic disease, or chronic cholestasis. Other causes of malabsorption of vitamin E include celiac disease, cystic fibrosis, and intestinal lymphangiectasia.

Patient Preparation: A fasting sample is preferred.

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Specimen Stability: Room Temperature: 24 Hours Refrigerated: 7 Days Frozen: 1 Year Test Code: CHANTILLY TC 931

Processing: Centrifuge within one hour and aliquot into dark brown aliquot tubes to protect from light. Freeze Transport: Transport frozen on ice

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: Next Day Test Schedule: Tuesday, Thursday Night Method: Radiobinding Assay Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Vitamin B12 Binding Capacity, Unsaturated Transcobalamin), binds and VitB12 Bind transports Vitamin B12 in the circulation. Increased concentrations are associated with patients with myeloproliferative disorders. Decreased concentrations are associated with infants with megaloblastic anemia or Transcobalamin deficiency.

Specimen Stability: Room Temperature: 4 Days Refrigerated: 4 Days Frozen: 30 days Test Code: CHANTILLY TC 928

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Transport: Refrigerate if held.

Preferred Collection/Volume: Collect urine with 25 mL 6N HCl to maintain a pH below 3 during collection. 24-Hour Urine Jug Volume: 10.0 mL Urine

TAT: 2 Days Test Schedule: Monday-Friday Morning Method: High Performance Liquid Chromatography Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

VMA 24 Hour Urine Clinical Data: (VMA) is the major urinary product resulting from the metabolic degradation of catecholamine, norepinephrine and epinephrine. Elevated concentrations of VMA are commonly found in cases of catecholamine secreting tumors such as pheochromocytoma, neuroblastoma, and ganglioneuroma. Drugs which can interfere with testing, due to physiologic response: clonidine, L-dopa, methocarbamol, inhibitors, reserprine, salicylates.

Patient Preparation: Clinician Instructions Only: It is preferable for the patient to be off medications for 3 days before specimen collection. However, common antihypertensives (diuretics, ACE inhibitors, calcium channel blockers, alpha and beta blockers) cause minimal or no interference. Patient should avoid alcohol, coffee, tea, tobacco (including use of nicotine patch), bananas, citrus fruits, and strenuous exercise before collection Labeling: Please specify on the test request form and on the urine container the total 24-hour urine volume. Record the patient's age on the test request form and on the urine container

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Specimen Stability: Room Temperature: 10 Days Refrigerated: 2 Weeks Frozen: 1 Year Test Code: CHANTILLY TC 22326

Processing: Mix specimen well and aliquot. Record total volume on container. Transport: Refrigerate if held.

Preferred Collection: Blue CIT Tube must be filled to minimum fill indicator Volume: 1mL plasma Neonates minimum volume plasma 100 µl, if not combined with other tests.

TAT: 2-7 days Test Schedule: Once per week Method: Immunoturbidometric Test Facility: AWL Von Willebrand Factor Antigen Patient Preparation: If using a butterfly, a citrate discard tube must be used first, when citrate is the first tube collected.

Processing centrifuge must be checked periodically for production of platelet poor plasma. Refer to W.I. 1.8B-19-200 Coagulation Specimen Quality Check

Processing: Centrifuge immediately after collection. Prepare platelet poor plasma. Freeze solid. Transport: Transport frozen on ice. DO NOT ALLOW TO THAW

Preferred Collection/Volume: BLUE CIT Volume: Full Tube

TAT: Next day Test Schedule: Mon-Fri a.m Method: Platelet Agglutination Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: V on Willebrand disease is the most common hereditary bleeding disorder. von Von Willebrand Factor Willebrand Factor is necessary for platelet adhesion to injured endothelium. Ristocetin cofactor is Assay useful in assessing binding of von Willebrand Factor to platelet factor GP1b. When combined with other tests, results are useful in categorizing the type of von Willebrand disease.

Specimen Stability: CRITICAL FROZEN DO NOT THAW. Test Code: Quest TC 34173

Processing: Centrifuge for 15 minutes at approximately 1500 g within 60 minutes of collection. Use plastic pipette to remove plasma, taking care to avoid the WBC/platelet buffy coat and place into an aliquot tube. Centrifuge a second time and pipette using a plastic pipette plasma into a Referred Tests aliquot tube. Freeze. Transport: Transport frozen on ice.

Preferred Collection/Volume: Blue Citrate Volume: 1.0 mL p

TAT: 3 Days Test Schedule: Mon-Thu a.m Method: Electrophoresis

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Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: von Willebrand disease is the most common hereditary bleeding disorder; it may also be acquired. von Willebrand Factor is necessary for platelet adhesion to injured endothelium. von Willebrand Factor Antigen, Multimeric Analysis is useful when type 2 disease is suspected and to further categorize disease.

Transport frozen on dry ice. Von Willebrand Factor TC# 7 47963 Multimer Assay Processing: Centrifuge for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately Transport: Transport frozen on ice.

Von Willebrand Factor Multimer Assay (85247A EAP, Von Willebrand Factor Multimer Assay, F8X, VWF)

Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 2.0 mL Serum Alternative Collection: LAV EDTA or GREEN NA HEP Volume: 2.0 mL Plasma

TAT: 1 to 6 Days Test Schedule: Tuesday, Thursday, and Saturday Method: Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry Test Facility: ARUP Laboratories 500 Chipeta Way, Salt Lake City, Utah 84108-1221 (800) 242-2787, ext. 5126

Clinical Data: Voriconazole is a new second-generation triazole antifungal agent. It has been shown to have 10-500 times more potent activity against a broad spectrum of clinically significant fungal pathogens in immunocompromised patients, including fluconazole-resistant Candida and Aspergillus species, as well as emerging fungal pathogens, such as Scedosporium and Fusarium species, which may be resistant to itraconazole, fluconazole, and amphotericin B. Voriconzaole is Voriconazole Level, predominantly metabolized in the liver by the cytochrome P450 enzyme system, mainly by the HPLC isozyme CYP2C19. Because of a genetic disposition towards a greater metabolism of the drug by certain population groups, a less effective metabolism by those with hepatic impairment, and because co-administration of other drugs may function to increase or decrease the systemic concentration of voriconazole, it may be clinically helpful to assay the serum/plasma level of voriconazole. Pharmacokinetic data to this point indicate that achievable steady state levels range from 2 to 6 mcg/mL depending upon the dosing used. The usual standard dosage is 200 mg BID PO or 4 mg/kg IV, but this can vary.

Patient Preparation: Specimens collected just before or within 15 minutes of the next dose represent the TROUGH levels. Specimens obtained within 15-30 minutes after the end of I.V. infusion or 45-60 minutes after an IM injection or 90 minutes after oral intake represent the PEAK level. Labeling: Label tube as Peak or Trough

Specimen Stability: Room Temrperature: 48 hours Refrigerated: 48 hours Frozen: 6 months Cause for Rejection: Separator tubes

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Hemolyzed or lipemic specimens TC# 2001737

Processing: Centrifuge and aliquot into Referred Tests aliquot tube. Note if serum or plasma on specimen. Freeze. Transport: Transport frozen on ice.

Preferred Collection/Volume: Rectal: Liquid Amies transport swab (E-swab) Alternative Collection: Fresh stool in leakproof container or enteric pathogen vial filled to line

TAT: 2 - 3 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: This test is used for screening for carriage of Vancomycin-resistant Enterococcus (VRE) only. VRE Culture Susceptibilities will not be performed. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Expired collection/transport devices >48 hours post collection

Processing: Refrigerate Transport: Send on Ice

Preferred Collection/Volume: Green 3 Sodium Heparin/ 3cc MINIMUM VOLUME 1CC Alternative Collection: n/a

TAT: Routine: 14-21 days Test Schedule: Mon-Fri Method: In situ hybridization on metaphase cells Test Facility: AWL

Williams ELN Mutation Clinical Data: If ordered in combination with a chromosome study, only 1 specimen is needed. (7q11.23) Analysis FISH Include clinical indication. Patient History: n/a

Patient Preparation: n/a Labeling: n/a

Processing: ** No Gel barrier tubes ** Do not Centrifuge. Hold at Room temperature. Do NOT reject centrifuged specimen; contact department at 503-258-6723 (Tie Line: 35-6723) Transport: Room Temperature.

Preferred Collection: Place worm in sterile leak-proof container. Add 70% alcohol to cover the worm. Alternative Collection: Submit worm or stool containing worm in parasite collection kit.

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TAT: 1 - 2 Days Test Schedule: Daily Method: Microscopy Testing Location: AWL Worm Identification Rejection Citeria: Dehydrated specimen

Processing: If stool received: separate worm from stool, place worm into sterile container, cover with 70 % alcohol. Transport: Hold and transport at room temperature.

Preferred Collection/Volume: Liquid Amies Transport Swab (E-swab)

TAT: 5 -14 Days Test Schedule: Daily Method: Culture Test Facility: Airport Way Regional Laboratory

Clinical Data: Please indicate source on specimen This culture includes aerobic and anaerobic organisms. Wound Culture, Deep Order Wound Culture, Superficial Aerobic, if source is superficial Aerobic/Anaerobic Please indicate source on specimen. Transport promptly to laboratory for processing.

Rejection Criteria: Sample submitted >48 hours post collection Expired collection/transport device Dry Swab

Processing: Hold at room temperature Transport: Transport promptly to Airport Way Regional Laboratory for processing

Preferred Collection/Volume: Liquid Amies Transport Swab (E-swab) Alternative Collection: n/a

TAT: 3 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: Specific source must be provided. This culture is aerobic only, for anaerobic organisms see Wound,Deep/Surgical Wound Culture, Patient History: n/a Superficial Aerobic Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Greater than 48 hours post collection. Expired collection/transport device. Dry Swab.

Processing: Hold at room temperature Transport: Room temperature

Avoid use of tourniquet. The patient must not clench their hand. If not possible to draw patient Xtra Grey without use of tourniquet, draw specimen immediately after application of tourniquet and release it immediately after venipuncture

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Processing: Centrifuge ASAP, aliquot, and store refrigerated

Preferred Collection/Volume: RD10

USE THIS PANEL TO TRACK EXTRA SERUM SPECIMEN COLLECTION WHEN ORDERS DO Xtra Red 10 NOT EXIST

Processing: Centrifuge and aliquot ASAP. Transport to AWL. CORE LAB: Refrigerate

Preferred Collection/Volume: Fresh Stool Specimen: Place a portion ( > 2 grams) of the stool specimen into the Enteric Pathogen Transport Vial within 2 hours of collection. Alternative Collection: Rectal Swab: Liquid Amies Transport swab (E-Swab)

TAT: 3 - 5 Days Test Schedule: Daily Method: Culture Test Facility: AWL

Clinical Data: Yersinia Culture should always be ordered with a Stool Culture Patient History: n/a

Yersinia Culture Patient Preparation: n/a Labeling: n/a

Rejection Criteria: Enteric Pathogen vial > 72 hours post collection Fresh stool > 2 hours post collection Rectal Swab > 48 hours post collection Expired collection/transport device Specimen contaminated with urine Specimen received in diaper

Processing: Refrigerate Transport: Send on Ice

Preferred Collection/Volume: Collect in a 24-hour ACID WASHED urine containe Volume: 7 mL urine Collect without preservative. Random urine is not acceptable.

TAT: Next day Test Schedule: Monday-Wednesday Afternoon Thursday, Friday Morning, Night Method: Atomic Spectroscopy (AS) Test Facility: Quest

Clinical Data: Zinc is an essential element involved in a myriad of enzyme systems, including wound healing, immune function, and fetal development. Zinc measurements are used to detect Zinc 24 Hour Urine and monitor industrial, dietary, and accidental exposure to zinc. Also, Zinc measurements may be used to evaluate health and monitor response to treatment.

Labeling: Write Total Volume on Label

Specimen Stability: Room temperature: 5 days Refrigerated: 14 days Frozen: 30 days Test Code: Quest TC 946

Processing: After collection measure total volume, mix thoroughly, aliquot into acid washed aliquot container . Note on sample total volume Transport: Refrigerate if held.

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Preferred Collection/Volume: Dark Blue EDTA Volume: 2.0 mL Plasma (Min0.7 mL)

TAT: Next Day Test Schedule: Wednesday, Friday, Sunday Morning Method: Atomic Spectroscopy Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Data: Zinc is an essential element involved in a myriad of enzyme systems, including wound healing, immune function, and fetal development. Zinc measurements are used to detect and monitor industrial, dietary, and accidental exposure to zinc. Also, zinc measurements may be Zinc Level used to evaluate health and to monitor response to treatment.

Specimen Stability: Room Temperature: 5 Days Refrigerated: 10 Days Frozen: 30 Days Rejection criteria: Hemolysis; Plasma not seperated from cells within two hours; Nontrace metal certified containers. Test Code: CHANTILLY TC 945

Processing: Centrifuge and aliquot into trace metal free aliquot tubes within 2 hours. Transport: To AWL in blue tote

Preferred Collection/Volume: LAV EDTA Volume: 2.0 mL Whole Blood Absolute Minimum Volume: .5mL Whole Blood Alternative Collection: Drk BLU EDTA, DrkGRN Na or Li Hep

TAT: Next Day Test Schedule: Monday-Friday Morning Method: Hematofluorometric Test Facility: Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Clinical Significance Zinc protoporphyrin (ZPP) accumulates in erythrocytes as a result of chronic lead absorption or iron deficiency anemia. Zinc Protoporphyrin Alternative Names(s) Free Erythrocyte Protoporphyrin Limitations Aluminum and anemia of chronic disease may cause elevated results

Specimen Stability: Room Temperature: 4 Days Refrigerated : 10 Days Frozen: Unacceptable TC# 948 Rejection Criteria: Hemolysis-Frozen-Clotted

Processing: Do Not Centrifuge. Protect from light. Wrap sample in foil. Transport: Refrigerate if held.

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Preferred Collection/Volume: RED 10 No Gel Barrier Tubes Volume: 1.0 mL Serum

TAT: 3 to 4 days Test Schedule: 3 days a week, night Method: Liquid Chromatography, Tandem Mass Spectrometry Test Facility: Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano, CA 92690-6130

Clinical Data: Zonisamide is comonly used as an adjunct together with other conventional anticonvulsants. As multiple drugs are administered, it is important to monitor its level to optimize therapeutic effects, to assure compliance, and to avoid toxicity. Zonisamide Level Patient Preparation: Draw sample 1/2 to 1 hour before next dose.

Specimen Stability: Room temperature: 24 Hours Refrigerated: 14 Days Frozen: 15 Months Reject Hemolysis: Yes Reject Lipemia: Yes Reject Thaw/Other: Specimen collected in gel barrier tube, Improperly labeled specimen, Serum Separator Tube Test Code: SJC TC 37852Z

Processing: Centrifuge and aliquot info Referred Tests aliquot tube. Transport: Refrigerate if held

Preferred Collection/Volume: RD10/ 2.0 mL s Alternative Collection: No gel barrier tubes/ 1.0 mL s

TAT: 4 Days Test Schedule: Mon, Thur Method: Chemiluminescent Immunoassay ZU (OHSU) Parathyroid Hormone Test Facility: AWL PTH for OHSU patients only. For cyst fluid order a CPH. Process like serum

Processing: Process like serum. Centrifuge, Aliquot, freeze. Transport: Transport on ice. Do not allow specimens to thaw.

Preferred Collection/Volume: RD10/ 1.0 mL Alternative Collection: SSRED5/ 0.4 mL

TAT: 24 Hours Test Schedule: Mon - Fri Method: Luminescent Immunoassay ZU Hep A Ab Total Test Facility: AWL

For OHSU patients only. This is a Hep A Ab, Total (IgM and IgG combined). This panel will automatically reflex a HAS (Hep A Ab,IgM)if result is positive. For Kaiser patients, order a HAR panel (no reflex).

Processing: Centrifuge, aliquot, refrigerate

Preferred Collection/Volume: RD10/ 1.0 mL Alternative Collection: SSRED5/ 0.2 mL

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TAT: 24 Hours Test Schedule: Mon - Fri Method: Luminescent Immunoassay ZU Hep B Core Ab Test Facility: AWL Total OHSU only, does not reflex HIG. For Non-OHSU order HBC

Processing: Cent, Pour, Refrigerate

Preferred Collection/Volume: Amniotic Fluid/ 2.0 mL Alternative Collection: / 1.0 mL

TAT: Daily ZU Prenatal Screen Test Schedule: Mon-Fri Amniotic Fluid Method: Immunoassay Test Facility: AWL

Processing: Refrigerate

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