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Anti-HLAB Antibody (PE-Cy7) Product Number: AC12-0704-17

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Anti-HLAB Antibody (PE-Cy7) Product Number: AC12-0704-17 Anti-HLAB Antibody (PE-Cy7) Product Number: AC12-0704-17 Overview Host Species: Mouse Clonality: Monoclonal (EP-4) Purity: Antibody is purified from bioreactor concentrate. The antibody is then conjugated to the PE-Cy7 tandem conjugate (phycoerythrin-cyanine 7). Isotype: IgM, kappa Conjugate: PE-Cy7 Immunogen Type: Anti-HLAB antibody was raised against lymphocytes from an HLA-B27 patient. Physical Characteristics Amount: 0.05 mg Concentration: 1 mg/ml Volume: 0.05 ml Buffer: Antibody is purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS at 1.0mg/ml. Storage: PE-Cy7 conjugated antibody can be stored at 4?C for up to 3 months. For longer storage the conjugate can be stored at -20?C after adding 50% glycerol. PE-Cy7 conjugated antibodies should always be stored in the dark. Specificity Species Reactivity: Human Specificity: This antibody recognizes the HLA-B27 cell surface antigen on human cells. It may be used to HLA type human lymphocytes. Approximately 60% of patients with ankylosing spondylitis are HLA-B27 positive. This reagent can be used to help identify this HLA haplotype in human lymphocytes. Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind pep- tides and present them to T lymphocytes. Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens: class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-B encodes a membrane anchored heavy chain, which hetero-dimerizes with a light chain (? -2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-B alleles. The HLA-B27 allele appears with increased frequency in uveitis patients. Target Information Accession Number(s): Swissprot: P01889 (Human) Gene ID Number(s): 77961 (Human) Alternative Names: AS; HLA B protein; HLA class I histocompatibility antigen; B alpha chain; HLA-B; HLA-B histocompatibility type; HLAB; leukocyte antigen class I-B; lymphocyte antigen; major histocompatibility complex; class I; B; MHC class I antigen GN00104; MHC class I antigen HLA-B heavy chain; MHC class I antigen SHCHA; MHC Class I HLA heavy chain; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; SPDA1 Application Details Tested Applications: IF, FC Immunoflourescence: Recommended starting concentration for immunofluorescence is 0.5-1 ug/ml. Page 1/2 All products are for research purposes only, not for diagnostic or therapeutic use. Abcore . 405 Maple Street, Suite A106 . Ramona, CA 92065 . T 888-358-1364 . F 888-884-9241 . www.abcore-inc.com Flow Cytometry: Recommended starting concentration for flow cytometry is 0.5-1 ug/million cells. Page 2/2 All products are for research purposes only, not for diagnostic or therapeutic use. Abcore . 405 Maple Street, Suite A106 . Ramona, CA 92065 . T 888-358-1364 . F 888-884-9241 . www.abcore-inc.com Powered by TCPDF (www.tcpdf.org).
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