DOCSLIB.ORG

Primary Productivity Below the Seafloor at Deep-Sea Hot Springs

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Primary Productivity Below the Seafloor at Deep-Sea Hot Springs Primary productivity below the seafloor at deep-sea hot springs Jesse McNichola,1,2, Hryhoriy Stryhanyukb, Sean P. Sylvac, François Thomasa,3, Niculina Musatb, Jeffrey S. Seewaldc, and Stefan M. Sieverta,1 aBiology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543; bDepartment of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research – Umweltforschungszentrum (UFZ), 04318 Leipzig, Germany; and cMarine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 Edited by David M. Karl, University of Hawaii, Honolulu, HI, and approved May 16, 2018 (received for review March 13, 2018) Below the seafloor at deep-sea hot springs, mixing of geothermal but only one such measurement has been previously obtained under fluids with seawater supports a potentially vast microbial ecosys- realistic temperature and pressure conditions (4). Another im- tem. Although the identity of subseafloor microorganisms is largely portant consideration is that electron acceptors such as oxygen and known, their effect on deep-ocean biogeochemical cycles cannot nitrate rapidly become limiting during incubation experiments with be predicted without quantitative measurements of their meta- vent fluids (13, 14), which may lead to carbon fixation rates being bolic rates and growth efficiency. Here, we report on incubations greatly underestimated (13). However, it is difficult to ascertain the of subseafloor fluids under in situ conditions that quantitatively extent of this bias for existing studies because electron acceptor constrain subseafloor primary productivity, biomass standing stock, consumption has not typically been measured alongside carbon and turnover time. Single-cell-based activity measurements and 16S fixation. Theoretical estimates of primary productivity have also rRNA-gene analysis showed that Campylobacteria dominated car- been derived by combining geochemical measurements with ther- bon fixation and that oxygen concentration and temperature drove modynamic models (15, 16). However, these studies rely on a niche partitioning of closely related phylotypes. Our data reveal a very active subseafloor biosphere that fixes carbon at a rate of up to number of untested assumptions necessary to convert the available 321 μgC·L−1·d−1, turns over rapidly within tens of hours, rivals the energy into biomass. productivity of chemosynthetic symbioses above the seafloor, and To overcome these limitations, we used a well-studied low- significantly influences deep-ocean biogeochemical cycling. temperature hot spring known as Crab Spa at the 9°N hydro- thermal vent field on the East Pacific Rise (EPR) as a model deep-sea hydrothermal vents | chemosynthesis | Campylobacteria | ecophysiology | NanoSIMS Significance n 1977, the discovery of deep-sea hot springs revealed unusual The existence of a chemosynthetic subseafloor biosphere was Iecosystems vastly more productive than other regions in the immediately recognized when deep-sea hot springs were dis- energy-limited deep sea (1). This productivity is sustained by covered in 1977. However, quantifying how much new carbon is chemosynthetic microorganisms that harness chemical energy fixed in this environment has remained elusive. In this study, we made available when oxidizing seawater and reducing hydro- incubated natural subseafloor communities under in situ pres- thermal fluid mix. It has long been recognized that the habitat for sure/temperature and measured their chemosynthetic growth such organisms may extend far below the seafloor to vast regions efficiency and metabolic rates. Combining these data with fluid of the ocean crust where fluid mixing takes place (1). Fluids exiting flux and in situ chemical measurements, we derived empirical this subseafloor biosphere are enriched in microbial biomass rel- constraints on chemosynthetic activity in the natural environ- ative to surrounding seawater (2) and contain active microor- ment. Our study shows subseafloor microorganisms are highly ganisms (2–4) that are physiologically and metabolically diverse productive (up to 1.4 Tg C produced yearly), fast-growing – (5–7). Despite the early realization that subseafloor ecosystems (turning over every 17 41 hours), and physiologically diverse. likely contribute significantly to overall chemosynthetic primary These estimates place deep-sea hot springs in a quantitative productivity (1) and provide nutrition to the surrounding food- framework and allow us to assess their importance for global limited deep sea, their extent, productivity, and biological dy- biogeochemical cycles. namics remain poorly constrained (1, 2, 8). It has been generally Author contributions: J.M., J.S.S., and S.M.S. designed research; J.M., H.S., S.P.S., F.T., N.M., assumed that above-seafloor production (i.e., microbe-animal J.S.S., and S.M.S. performed research; N.M., J.S.S., and S.M.S. contributed new reagents/ symbiotic associations) exceeds production below the seafloor analytic tools; J.M., H.S., N.M., J.S.S., and S.M.S. analyzed data; and J.M., J.S.S., and S.M.S. (9). However, this assumption has not been rigorously tested by wrote the paper. empirically quantifying subseafloor productivity. The authors declare no conflict of interest. Quantifying subseafloor productivity at submarine hot springs This article is a PNAS Direct Submission. requires knowledge of both the amount of chemical energy that This open access article is distributed under Creative Commons Attribution-NonCommercial- can be supplied by hydrothermal fluids and the efficiency by NoDerivatives License 4.0 (CC BY-NC-ND). which microbial communities convert this chemical energy into Data deposition: The data reported in this paper have been deposited in the NCBI Se- biomass. Although the chemical composition of hydrothermal quence Read Archive (SRA) (https://www.ncbi.nlm.nih.gov/sra/?term=SRX1898689; acces- sion no. SRX1898689). fluids is well-described and provides strong indirect evidence for 1To whom correspondence may be addressed. Email: [email protected] or ssievert@ high biological activity in the subseafloor (10, 11), the actual whoi.edu. amount of carbon fixed in situ is highly uncertain. Although in 2Present address: University of Southern California, Department of Biological Sciences, Los situ growth yields can be estimated from pure cultures (12), re- Angeles, CA 90089. sults from laboratory cultures may not be relevant to complex 3Present address: Sorbonne Université, CNRS, Integrative Biology of Marine Models and largely uncultivated natural communities growing under (LBI2M), Station Biologique de Roscoff, 29680 Roscoff, France. different physical and chemical conditions. Bulk carbon fixation This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10. rates reported for mixed subseafloor microbial communities 1073/pnas.1804351115/-/DCSupplemental. could likewise be used to constrain subseafloor primary productivity, Published online June 11, 2018. 6756–6761 | PNAS | June 26, 2018 | vol. 115 | no. 26 www.pnas.org/cgi/doi/10.1073/pnas.1804351115 Downloaded by guest on October 3, 2021 Table 1. Predicted versus observed concentrations of potential electron donors and acceptors at Crab Spa + − 2− H2S, μmol/L H2, μmol/L CH4, μmol/L NH4 , μmol/kg O2, μmol/kg NO3 , μmol/L SO4 , mmol/kg Predicted* 552 29 8.1 0.2 107 32 25.8 Observed 184 <2 6.3 11.9 3.6 6.3 26.5 Units are given either per liter or kilogram of Crab Spa vent fluid. *Values are based on fluid mixing calculations previously described in ref. 14. system to constrain subseafloor chemosynthetic production. Microbial Community Composition and Rate Measurements. In all in- Data from incubations conducted under simulated in situ con- cubations, Campylobacteria (7) were the dominant microorganisms, ditions were combined with in situ chemistry and fluid flow rate as shown by catalyzed-reporter deposition fluorescence in situ hy- (14, 17) to arrive at empirical estimates of subseafloor primary bridization (CARD-FISH) cell counts [Fig. 1A and SI Appendix, productivity in a deep-sea hydrothermal system. Table S1;94± 11% of total cells (14)] and the proportion of 16S rRNA gene sequences (97 ± 3.7%; SI Appendix,Fig.S1). Identified Results and Discussion sequences were related to known chemolithoautotrophs, and a Fluids emanating from the subseafloor at Crab Spa are charac- pronounced switch from known mesophilic to thermophilic Cam- terized by a temperature of ∼24 °C, microbial abundances sub- pylobacteria occurred in the incubation at 50 °C (Fig. 1A and SI stantially elevated relative to bottom seawater, and a chemical Appendix,Fig.S1andTableS1). Active carbon fixation was con- 13 − composition for most aqueous species that reflects formation by firmed by specifically measuring H CO3 tracer incorporation in conservative subseafloor mixing of the high-temperature end- campylobacterial cells with halogen in situ hybridization-nanoscale member fluid with seawater (14). Dissolved concentrations of secondary ion mass spectrometry [HISH-SIMS (19); Fig. 2]. HISH- redox reactive sulfide, hydrogen, nitrate, and oxygen, however, are SIMS also demonstrated that amendments increased relative CO2 substantially depleted relative to values expected for conservative fixation rates, especially for oxygen or a combination of nitrate and hydrogen (Fig. 3). Given that Campylobacteria dominate the natural mixing (Table 1), indicating microbial consumption below the ∼ A seafloor (10, 11, 14). Sixteen independent incubation experiments
Load more

Recommended publications
  • Microbial Community Composition and Function Beneath Temperate Trees Exposed to Elevated Atmospheric Carbon Dioxide and Ozone
    Oecologia (2002) 131:236–244 DOI 10.1007/s00442-002-0868-x ECOSYSTEMS ECOLOGY Rebecca L. Phillips · Donald R. Zak William E. Holmes · David C. White Microbial community composition and function beneath temperate trees exposed to elevated atmospheric carbon dioxide and ozone Received: 1 August 2001 / Accepted: 13 December 2001 / Published online: 14 February 2002 © Springer-Verlag 2002 Abstract We hypothesized that changes in plant growth activity and microbial metabolism of cellobiose, and that resulting from atmospheric CO2 and O3 enrichment microbial processes under early-successional aspen and would alter the flow of C through soil food webs and birch species were more strongly affected by CO2 and O3 that this effect would vary with tree species. To test this enrichment than those under late-successional maple. idea, we traced the course of C through the soil microbial community using soils from the free-air CO2 and O3 Keywords Soil microorganisms · enrichment site in Rhinelander, Wisconsin. We added Carbon-13-phospholipid fatty acid analysis · either 13C-labeled cellobiose or 13C-labeled N-acetylglu- Elevated carbon dioxide · Elevated ozone · cosamine to soils collected beneath ecologically distinct Soil carbon cycling temperate trees exposed for 3 years to factorial CO2 –1 (ambient and 200 µl l above ambient) and O3 (ambient and 20 µl l–1 above ambient) treatments. For both labeled Introduction substrates, recovery of 13C in microbial respiration increased beneath plants grown under elevated CO2 by Human activity has increased the concentration of CO2 29% compared to ambient; elevated O3 eliminated this and O3 in the earth’s troposphere (Barnola et al.
    [Show full text]
  • Assessing the Chemistry and Bioavailability of Dissolved Organic Matter from Glaciers and Rock Glaciers
    RESEARCH ARTICLE Assessing the Chemistry and Bioavailability of Dissolved 10.1029/2018JG004874 Organic Matter From Glaciers and Rock Glaciers Special Section: Timothy Fegel1,2 , Claudia M. Boot1,3 , Corey D. Broeckling4, Jill S. Baron1,5 , Biogeochemistry of Natural 1,6 Organic Matter and Ed K. Hall 1Natural Resource Ecology Laboratory, Colorado State University, Fort Collins, CO, USA, 2Rocky Mountain Research 3 Key Points: Station, U.S. Forest Service, Fort Collins, CO, USA, Department of Chemistry, Colorado State University, Fort Collins, • Both glaciers and rock glaciers CO, USA, 4Proteomics and Metabolomics Facility, Colorado State University, Fort Collins, CO, USA, 5U.S. Geological supply highly bioavailable sources of Survey, Reston, VA, USA, 6Department of Ecosystem Science and Sustainability, Colorado State University, Fort Collins, organic matter to alpine headwaters CO, USA in Colorado • Bioavailability of organic matter released from glaciers is greater than that of rock glaciers in the Rocky Abstract As glaciers thaw in response to warming, they release dissolved organic matter (DOM) to Mountains alpine lakes and streams. The United States contains an abundance of both alpine glaciers and rock • ‐ The use of GC MS for ecosystem glaciers. Differences in DOM composition and bioavailability between glacier types, like rock and ice metabolomics represents a novel approach for examining complex glaciers, remain undefined. To assess differences in glacier and rock glacier DOM we evaluated organic matter pools bioavailability and molecular composition of DOM from four alpine catchments each with a glacier and a rock glacier at their headwaters. We assessed bioavailability of DOM by incubating each DOM source Supporting Information: with a common microbial community and evaluated chemical characteristics of DOM before and after • Supporting Information S1 • Data Set S1 incubation using untargeted gas chromatography–mass spectrometry‐based metabolomics.
    [Show full text]
  • Discovery of Chemosynthesis-Based Association on the Cretaceous Basal Leatherback Sea Turtle from Japan
    Editors' choice Discovery of chemosynthesis-based association on the Cretaceous basal leatherback sea turtle from Japan ROBERT G. JENKINS, ANDRZEJ KAIM, KEI SATO, KAZUHIRO MORIYA, YOSHINORI HIKIDA, and REN HIRAYAMA Jenkins, R.G., Kaim, A., Sato, K., Moriya, K., Hikida, Y., and Hirayama, R. 2017. Discovery of chemosynthesis-based association on the Cretaceous basal leatherback sea turtle from Japan. Acta Palaeontologica Polonica 62 (4): 683–690. We report a Late Cretaceous chemosynthetic community fueled by decomposing basal leatherback sea turtle on the ocean floor in the western Pacific. The fossil association representing this community has been recovered from the matrix of a concretion containing a single carapace of Mesodermochelys sp. from Late Cretaceous outer shelf to upper slope deposit of northern Hokkaido, Japan. The carapace displays boreholes most likely performed by boring bivalves, and is associated with molluscan shells, mainly Provanna cf. nakagawensis and Thyasira tanabei. Since this association is similar to fauna already known from Late Cretaceous hydrocarbon seeps, sunken wood, and plesiosaur-falls in Hokkaido, it is suggested that all types of chemosynthesis-based communities in the Late Cretaceous of western Pacific may have belonged to the same regional pool of animals and were not yet fully differentiated into three independent types of com- munities as it is known today. This finding also indicates that the sulfophilic stage of the vertebrate-fall communities was supported not only by plesiosaur carcasses, which were previously reported, but also by sea turtle carcasses. It highlights the possibility of surviving vertebrate-fall communities through the end-Cretaceous mass extinction event on carcasses of sea turtles which are the only large marine vertebrates surviving this event.
    [Show full text]
  • Microbial Metabolism, Chemistry, and Communities Under Study at the UCLA-DOE Institute for Genomics and Proteomics Rachel R
    Microbial Metabolism, Chemistry, and Communities under Study at the UCLA-DOE Institute for Genomics and Proteomics Rachel R. Ogorzalek Loo* ([email protected]), John Muroski, Brendan Mahoney, Orlando Martinez, Janine Fu, Joseph A. Loo, Robert Clubb, Robert Gunsalus, and Todd Yeates 1UCLA-DOE Institute for Genomics and Proteomics, Los Angeles, CA https://www.doe-mbi.ucla.edu/ Project Goals: Research in the UCLA-DOE Institute for Genomics and Proteomics includes major efforts to elucidate critical microbial processes that decompose and recycle plant, animal and microbial biomass. Towards this end, we seek to decipher the metabolism of syntrophic microbial communities and examine how anaerobic microbes assemble complex cellulosome structures that degrade lignocellulose. Biomass decomposition and recycling occur in essentially all anaerobic habitats on Earth, as well as in industrial/municipal waste treatment applications. Unfortunately, the current understanding of these critical processes is insufficient to enable modeling and prediction of environmental carbon flow. The benefits from increasing our knowledge of anaerobic decomposition include optimizing the attack and release of plant wall-derived molecules destined for biofuel and industrial feedstock production and improving biogas/sewage and waste stream processing plant design and operation. Our DOE sponsored research seeks to advance the understanding of syntrophic-based microbial metabolism at molecular- and systems-levels and its role in biomass recycling/remediation. Exploring the pathways and key enzyme reactions of syntrophy begins by mining the genomes of previously unstudied syntrophic bacteria, such as those that metabolize model aliphatic fatty acid and amino acid substrates. That only minimal experimental data pertaining to these organisms is available severely limits the ability to draw conclusions from genome sequence alone, and even adding transcriptomic data may not suffice.
    [Show full text]
  • Chapter 5 Hmdscience.Com EN Online Vir Onmental Science Work Ecosystems How
    DO NOT EDIT--Changes must be made through “File info” printcode=a Chapter 5 Section 1 Energy Flow in Ecosystems How Section 2 The Cycling of Matter Section 3 How Ecosystems Change Why It Matters Ecosystems This frog gets the energy it needs to survive by eating other organisms, such as damselflies. Frogs and damselflies are both consumers in an aquatic food chain. Work How does energy continue to be transferred in this food chain? CASESTUDY Learn how pollutants, like the pesticide DDT, are transferred through a food chain in the case study DDT in an Aquatic Food Chain on page 120. Online enVirOnmental Science HMDScience.com Go online to access additional resources, including labs, worksheets, multimedia, and resources in Spanish. Inc. Cosmos Blank/Photo Researchers, ©A. 116 DO NOT EDIT--Changes must be made through “File info” printcode=a Section 1 Energy Flow in Objectives Describe how energy is transferred from the sun Ecosystems to producers and then to consumers. organisms need energy to survive, grow, and reproduce. Different organisms Describe one way in which get energy from different sources, but the ultimate source of energy for almost all consumers depend on producers. organisms on earth is the sun. Identify two types of consumers. Explain how energy transfer in a Life Depends on the Sun food web is more complex than Energy from the sun enters an ecosystem when organisms use sunlight energy transfer in a food chain. to make sugar in a process called photosynthesis. During photosynthesis, plants, algae, and some bacteria capture light energy from the sun and Explain why an energy pyramid use it to convert carbon dioxide and water into sugar and oxygen, as is a representation of trophic shown in Figure 1.1.
    [Show full text]
  • Chemosynthesis: What It We Can Learn from Hydrothermal Vents
    Chemosynthesis:Chemosynthesis: WhatWhat itit wewe cancan learnlearn fromfrom hydrothermalhydrothermal ventsvents Ryan Perry Geol 062 II.. IInnttrroo ttoo MMeettaabboolliissmm 1. CCaarrbboonn fifixxaattiioonn aanndd PPhhoottoossyynntthheessiiss 2. FFaammiilliiaarr ooxxiiddaattiivvee mmeettaabboolliissmm 3. OOxxyyggeenniicc PPhhoottoossyynntthh.. 4. GGeeoollooggiicc ccoonnsseeqquueenncceess IIII.. CChheemmoossyynntthheessiiss 1. HHyyddrrootthheerrmmaall VVeennttss 2. AArrcchheeaann 3. CChheemmoossyynntthheettiicc mmeettaabboolliissmm:: MMiiccrroobbeess RRuullee!!!!!! 4. CChheemmoossyynntthheettiicc eeccoossyysstteemmss IIIIII.. WWhhyy aarree eexxttrreemmoopphhiilleess ssoo ccooooll?? 1. BBiioommeeddiiccaall 2. IInndduussttrriiaall 3. WWhhaatt eexxttrreemmoopphhiilleess tteeaacchh uuss aabboouutt eeaarrllyy lliiffee 4. EExxoobbiioollooggyy IIVV.. EExxoobbiioollooggyy PPrreebbiioottiicc CChheemmiissttrryy oonn EEaarrtthh PPoossssiibbllee ((pprroobbaabbllee??)) oorriiggiinnss ooff lliiffee.. PPoossssiibbiillee lliiffee eellsseewwhheerree iinn tthhee ssoollaarr ssyysstteemm.. MMeettaabboolliissmm • The complete set of chemical reactions that take place within a cell. • Basis of all life processes. • Catabolic and Anabolic MMeettaabboolliissmm • CCaattaabbllooiicc mmeettaabboolliissmm---- hhiigghh eenneerrggyy mmoolleeccuulleess ((eelleeccttrroonn--ddoonnoorrss,, ffoooodd)) aarree ooxxiiddiizzeedd,, hhaavviinngg tthheeiirr eelleeccttrroonnss ttrraannssffeerrrreedd ttoo aann eelleeccttrroonn--aacccceeppttoorr.. • EElleeccttrroonn ppaasssseess ddoowwnn
    [Show full text]
  • The Chemosynthetic Cafe
    ocean INSPIRE: Chile Margin 2010 The Chemosynthetic Cafe www.oceanexplorer.noaa.gov Focus Chemosynthesis in hydrothermal vent ecosystems Grade Level 9-12 (Biology/Chemistry) Focus Question How is energy obtained and transferred in photosynthesis and chemosynthesis, and how are these processes similar and different? Learning Objectives n Students will compare and contrast photosynthesis and chemosynthesis. n Students will define oxidation and reduction as these terms apply to electron transfer. n Students will explain the overall process by which energy is captured and transferred during photosynthesis and chemosynthesis. Materials q None Audio-Visual Materials q (Optional) video or computer projection equipment to show images from the INSPIRE: Chile Margin 2010 Web page (http:// oceanexplorer.noaa.gov/explorations/10chile/welcome.html) Teaching Time Two 45-minute class periods, plus time for student assignments Seating Arrangement Groups of 3-4 students Maximum Number of Students 32 Image captions/credits on Page 2. Key Words Hydrothermal vent Autotroph Photosynthesis 1 www.oceanexplorer.noaa.gov INSPIRE: Chile Margin 2010: The Chemosynthetic Cafe Grades 9-12 (Biology/Chemistry) Chemosynthesis Electron transport Chile Triple Junction Background Information NOTE: Explanations and procedures in this lesson are written at a level appropriate to professional educators. In presenting and discussing this material with students, educators may need to adapt the language and instructional approach to styles that are best suited to specific student groups. Earthquakes and volcanoes are among Earth’s most spectacular and terrifying geological events. The Mount St. Helens eruption of 1980 Images from Page 1 top to bottom: Map of the Southeast Pacific Ocean and South and the Haiti (7.0 magnitude) and Chile (8.8 magnitude) earthquakes American continent showing the Chile Rise spreading center, the Peru-Chile Margin, and of 2010 are recent and memorable examples of the extreme power the location of the Chile Triple Junction.
    [Show full text]
  • Microbial Lithification in Marine Stromatolites and Hypersaline Mats
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by RERO DOC Digital Library Published in Trends in Microbiology 13,9 : 429-438, 2005, 1 which should be used for any reference to this work Microbial lithification in marine stromatolites and hypersaline mats Christophe Dupraz1 and Pieter T. Visscher2 1Institut de Ge´ ologie, Universite´ de Neuchaˆ tel, Rue Emile-Argand 11, CP 2, CH-2007 Neuchaˆ tel, Switzerland 2Center for Integrative Geosciences, Department of Marine Sciences, University of Connecticut, 1080 Shennecossett Road, Groton, Connecticut, 06340, USA Lithification in microbial ecosystems occurs when pre- crucial role in regulating sedimentation and global bio- cipitation of minerals outweighs dissolution. Although geochemical cycles. the formation of various minerals can result from After the decline of stromatolites in the late Proterozoic microbial metabolism, carbonate precipitation is pos- (ca. 543 million years before present), microbially induced sibly the most important process that impacts global and/or controlled precipitation continued throughout the carbon cycling. Recent investigations have produced geological record as an active and essential player in most models for stromatolite formation in open marine aquatic ecosystems [9,10]. Although less abundant than in environments and lithification in shallow hypersaline the Precambrian, microbial precipitation is observed in a lakes, which could be highly relevant for interpreting the multitude of semi-confined (physically or chemically)
    [Show full text]
  • Chemosynthesis for the Classroom (Adapted from the Expedition to the Deep Slope 2006)
    o c e a n Expl ration & Research Lessons from the Deep: Exploring the Gulf of Mexico’s Deep-Sea Ecosystems Education Materials Collection Chemosynthesis for the Classroom (adapted from the Expedition to the Deep Slope 2006) Focus Chemosynthetic bacteria Grade Level 9-12 (Chemistry/Biology) Focus Question What changes affect succession in the development of chemosynthetic bacterial communities? Learning Objectives m Students will observe the development of chemosynthetic bacterial communities. m Students will recognize that organisms modify their environment in ways that create opportunities for other organisms to thrive. m Students will be able to explain the process of chemosynthesis. m Students will be able to explain the relevance of chemosynthesis to biological communities in the vicinity of cold seeps. Materials m Directions for Setting Up Winogradsky Columns, one copy for each student group Materials for Winogradsky columns for each student group: m 2 1-liter plastic soda bottles m 1 Liter black mud from a local river, lake, or estuary m 1 Liter water from each mud/sand location used Image captions/credits on Page 2. m 1 Small bucket m 1 500 ml plastic beaker m 1 Paint stirrer or large spoon m 1 Sheet of newspaper 1 www.oceanexplorer.noaa.gov Lessons from the Deep: Exploring the Gulf of Mexico’s Deep-Sea Ecosystems Chemosysthesis for the Classroom - Grades 9-12 (Chemistry/Biology) m 1 Tablespoon powdered chalk (see Learning Procedure, Step 1d) m 1 Crushed multivitamin pill m Calcium sulfate (Plaster of Paris), approximately 80 g
    [Show full text]
  • Chapter 13 Lecture
    ChapterChapter 1 13 Clickers Lecture Essentials of Oceanography Eleventh Edition Biological Productivity and Energy Transfer Alan P. Trujillo Harold V. Thurman © 2014 Pearson Education, Inc. Chapter Overview • Primary productivity is photosynthesis. • Productivity is globally and seasonally variable. • Feeding relationships are represented by food chains and food webs. • Oceans are being overfished. © 2014 Pearson Education, Inc. Primary Productivity • Rate at which energy is stored in organic matter – Photosynthesis uses solar radiation. – Chemosynthesis uses chemical reactions. • 99.9% of the ocean’s biomass relies directly or indirectly on photosynthesis for food. © 2014 Pearson Education, Inc. Photosynthesis © 2014 Pearson Education, Inc. Measurement of Primary Productivity • Directly – capture plankton in plankton nets • Measure radioactive carbon in seawater © 2014 Pearson Education, Inc. Measurement of Primary Productivity • Monitor ocean color with satellites – Photosynthetic phytoplankton use green pigment chlorophyll • SeaWiFS (Sea-viewing Wide Field of View Sensor) satellite sensor collected ocean color data 1997–2010 • MODIS (Moderate Resolution Imaging Spectroradiometer) – current – Measures 36 spectral frequencies © 2014 Pearson Education, Inc. Factors Affecting Primary Productivity • Nutrient availability – Nitrate, phosphorous, iron, silica – Most from river runoff – Productivity high along continental margins – Redfield ratio – C:N:P © 2014 Pearson Education, Inc. Factors Affecting Primary Productivity • Solar radiation – Uppermost surface seawater and shallow seafloor – Compensation depth – net photosynthesis becomes zero – Euphotic zone —from surface to about 100 meters (330 feet) • Enough light for photosynthesis © 2014 Pearson Education, Inc. Light Transmission in Ocean Water • Visible light portion of the electromagnetic spectrum • Blue wavelengths penetrate deepest • Longer wavelengths (red, orange) absorbed first © 2014 Pearson Education, Inc. Transmission of Light in Seawater © 2014 Pearson Education, Inc.
    [Show full text]
  • Ocean Primary Production
    Learning Ocean Science through Ocean Exploration Section 6 Ocean Primary Production Photosynthesis very ecosystem requires an input of energy. The Esource varies with the system. In the majority of ocean ecosystems the source of energy is sunlight that drives photosynthesis done by micro- (phytoplankton) or macro- (seaweeds) algae, green plants, or photosynthetic blue-green or purple bacteria. These organisms produce ecosystem food that supports the food chain, hence they are referred to as primary producers. The balanced equation for photosynthesis that is correct, but seldom used, is 6CO2 + 12H2O = C6H12O6 + 6H2O + 6O2. Water appears on both sides of the equation because the water molecule is split, and new water molecules are made in the process. When the correct equation for photosynthe- sis is used, it is easier to see the similarities with chemo- synthesis in which water is also a product. Systems Lacking There are some ecosystems that depend on primary Primary Producers production from other ecosystems. Many streams have few primary producers and are dependent on the leaves from surrounding forests as a source of food that supports the stream food chain. Snow fields in the high mountains and sand dunes in the desert depend on food blown in from areas that support primary production. The oceans below the photic zone are a vast space, largely dependent on food from photosynthetic primary producers living in the sunlit waters above. Food sinks to the bottom in the form of dead organisms and bacteria. It is as small as marine snow—tiny clumps of bacteria and decomposing microalgae—and as large as an occasional bonanza—a dead whale.
    [Show full text]
  • 1 Students Will Grow and Observe Succession and Chemosynthesis Of
    GROWING CHEMOSYNTHETIC BACTERIA OVERVIEW Students will grow and observe succession and chemosynthesis of bacterial colonies: one lighted, the other in the dark. This activity uses the concepts of the Winogradsky column, a device which enriches and isolates certain organisms involved in the sulfur and nitrogen cycles. The activity provides a rough analog to both processes of chemosynthesis and succession; processes which occur at deep sea hydro- thermal vents and form the base of the food chain in the absence of sunlight. (Note that the original Winogradsky column was made by a Russian microbiologist Sergei N. Winogradsky.) CONCEPTS • Some organisms cannot draw energy from the Sun and must find other energy sources to live. • Both photosynthesis (uses sunlight) and chemosynthesis are means of producing sugar (stored chemical energy) • Photosynthetic organisms use light as their energy source; chemosynthetic organisms use chemi- cals. The result for both organisms is the same: energy. The by-products are different, however. In photosynthesis, oxygen is given off as a waste product. In chemosynthesis sulfate is given off. • As organisms thrive in a given environment their by-products create a new environment where new species thrive. This is called succession. MATERIALS For each group: • Black mud (enough to fill the graduated cylinders). See Preparation section. • 80 g of CaSO4 (Plaster of Paris: found in any hardware store) • 20 Jars or beakers for mixing • Stirring rods • Organic straw or filter paper bits. (Tear strips of lab filter paper) • 3 Liters (about 3 quarts) of pond, salty sea water or swamp water • 4 Grams baking soda • 20 Multivitamin pills and something with which to crush them • Plastic wrap • Rubber bands • A light source that can stay on for six weeks or longer • Tape and markers for labeling columns • Flashlight with red cellophane on lighted end (can be attached with rubber band) • Two 500 milliliter graduated cylinders or columns PREPARATION Divide the class into pairs or small groups.
    [Show full text]