Adriatic Flyway – Closing the gap in bird Conservation preface Dinaric Karst Poljes — Floods for Life Edited by: Peter Sackl, Romy Durst, Dražen Kotrošan and Borut Stumberger 1 Dinaric Karst Poljes – Floods for Life Edited by: Peter Sackl, Romy Durst, Dražen Kotrošan and Borut Stumberger Proceedings of the 1st Workshop on Karst Poljes as Wetlands of National and International Importance, Livno, 30 September - 1 October 2014 Olm Proteus anguinus (Photo: Gregor Aljančič) 70 Dinaric Karst Poljes – Floods for Life Workshop and project results Endangered Proteus: combining DNA and GIS analyses for its conservation Gregor Aljančič1, Špela Gorički1, Magdalena Năpăruş1, 2, David Stanković3 & Matjaž Kuntner4 1 Society for Cave Biology, Tular Cave Laboratory, Oldhamska c. 8a, SI-4000 Kranj, Slovenia, E-mail:
[email protected],
[email protected] 2 LASIG, École Polytechnique Fédérale de Lausanne, Station 18, CH-1015 Lausanne, Switzerland, E-mail:
[email protected] 3 University of Ljubljana, Biotechnical Faculty, Zootechnical Department, Groblje 3, SI-1230 Domžale, Slovenia, E-mail:
[email protected] 4 Institute of Biology, Scientific Research Centre, Slovenian Academy of Sciences and Arts, Novi trg 2, SI-1000 Ljubljana, Slovenia, E-mail:
[email protected] Summary Proteus subterranean habitat can be accessed by man. We are currently developing a method of detection of Proteus anguinus, an endemic amphibian of the traces of Proteus DNA in water samples (environmental subterranean waters of the Dinaric Karst, is restricted DNA); when integrated in an accurate distribution to its cave habitat. However, during seasonal flooding, Geographic Information System (GIS) model, the potential some specimens are washed-out of their subterranean pattern of its genetic variability within the complex karst environment.