I!Ilct of HOST PLANT on MEALYBUGS and THEIR
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1 I!iLCT OF HOST PLANT ON MEALYBUGS AND THEIR PARASITOIDS BY }LALAMBA ARACHCHIGE SUMANASIRI PERERA Thesis submitted for the degree of Doctor of Philosophy of the University of London Department of Biological Sciences Wye College University of London June 1990 2 DEDICATION Th13 work is dedicated to my parents and teachers who guided me to the Scientific World 3 ABSTRACT Six host plant species were tested as food plants for various life history parameters of two species of mealybug Planococous citri (Risso) and Pseudococcus affinis (Maskell). The range of acceptability Suggested that plants can be divided into host, acceptable non-host and unacceptable hosts. Mealybugs reared on soy bean plants developed significantly faster and reached a higher body weight in comparison to Insects reared on Gesneriaceae. Passiflora plant appeared to be completely resistant to P. cltri. Possible reasons for this are discussed. •The fecundity level of mealybugs reared on leguminous plants showed a two fold increase in comparison to Gesneriaceae plants. The relationship between fecundity, fresh weight and mean body length of adults showed differences between inealybugs reared on different plant species. The influence of host plant factors on the third trophic level was investigated. Anagyrus pseudococci (Girault) and Leptomastix dactylopil Howard were allowed to oviposit on P. cltri reared on various host plants and the fitness parameters were measured. Mealybugs fed on leguminous plants appear to be a better source or nutrient for the developing parasitoid larvae since the larger adults with longer life expectancy emerged from those hosts. More females emerged when the parasitolds oviposit on mealybugs reared on leguminous plants. The influence of host plant factors on the efficiency of encapsulation of eggs of A. pseudococci and L. dactylopli was studied under solitary and superparasitized conditions. Mealybugs reared on high phenolic plants such as Aeschynanthus ellipticus and Passiflora coccinea showed a significantly greater ability to encapsulate parasitoids eggs. 4 Over 90% of the eggs of L. dactylopii were encapsulated by P. affinis but '0% of the eggs of A. pseud000cci developed within the host body cavity. A proper immunity reaction was observed when parasitoids oviposit on P. citrl but no clear response was noted in P. affinis. The impact of plant water stress on the life history parameters of - mealybugs was investigated. A significant reduction in mean body size of mealybugs was recorded when they were reared on high stressed plants and as a consequence the fecundity level of the insect was drastically reduced. The parasitoids developed from smaller host showed reduced fitness parameters. Population parameters of mealybugs were compared on potato and coleus plants fertilized with different levels of nitrogen. Mealybugs fed on low nitrogen plants showed a significant reduction in fecundity and this may be the main reason f or the low rm value of mealybugs on low nitrogen plants. Some fitness parameters of parasitoids were not influenced by the level of nitrogen in the host plants. A study of searching behaviour of L. dactylopli on various plant species showed that trichomes of Streptocarpus and soy bean leaves can change the normal searching behaviour of the insect. Parasitoids walked short distances on Streptocarpus plants while the speed of movement and area covered during searching was higher on winged bean plants. 5 ACKNOWLEDGEMENTS In this study I received invaluable assistance and cooperation from many sources, and would like to express my sincere thanks to those who rendered assistance. I am greatly indebted to Dr. M.J.W. Copland for his continuous guidance, supervision and encouragements during my study period here at Wye. He was most helpful in organizing funds for my third year, without which I would not have been able to complete this study. I am appreciative for the continuous assistance offered by Dr. Chris Hodgson. His critical examination and suggestions for most of the experimental designs has enable me to gather more accurate knowledge about the subject. I am so grateful to the members of the academic and administrative staff of Wye College for their assistance in all possible ways to continue my studies to achieve my aspirations. Wye College Foundation fund and the Sir Ernest Cassel Educational Trust should accept my silent thanks f or the funds made available for my third year of studies. I reserve my special thanks to Professors J.H.D. Prescott, D.A. Baker and Mr. C.H. Martindale for their advice and helps. I would like to express my thanks to Mr. A. Clewer in the Department of Statistics for writing several statistical programs and guidance in analysing the data obtained in this study. 6 Appreciation is also extended to Dr. V.M. Koritsas and K.?. Martyn for their help in the chemical analysis of experimental samples. I am grateful to the members of the Wye College glasshouse staff for their assistance in maintaining experimental plants, and to all the technical staff in the Department, who have been so cooperative.. Delia Copland, Anne Hutcheon, Diana Prescott, Sister Tean Andrews, Bob, Kim and several other friends should accept my sincere thanks for the encouragements and helps to make our life happy in Wye. Finally, I would like to express my thanks to my wife and our daughter, for their tolerance, help and understanding throughout the period of this study. 7 CONTENTS DEDICATION. • . •. .2 ABSTRACT. 3 ACKNOWLEDGEMENTS ............. 5 CONTENTS....... 7 LIST OF TABLES. ........ .26 LIST OF FIGURES. .30 LIST OF PLATES. ........ .32 SECTION 1 EFFECT OF HOST PLANTS ON LIFE HISTORY PARAMETERS OF MEALYBUGS 1.1 INTRODUCTION...... .. •.... .33 1 .2 REVIEW OF LITERATURE...... • • • • • • • • • • • • . .35 1.2.1 Varietal differences or host plants and insect performance...... .35 1.2.2 Host plant morphological features and insects....,............35 1 .2.3 Influence or host plants on the developmental period of insects...... .. .. .. ...37 1 .2. Influence of host plant on the fecundity of herbivorous insects...... .. .38 1.2.5 Secondary plant substances and insect pertormance.............39 1.2.6 The experimental plants........................,..,.,.........lii 1.2.6.1 Passiflora caerulea . • •... ...., ,. ,.••.,,••••. .......... •. • 1.2.6.2 Passiflora coccinea .... .. ..,. .... ... ,••. .. ........ .... 1.2.6.3 PsophocarpustetragonolobusWingedbean............,.,,....••1j i.2.6.1 8 CONTENTS (cont.) 1.2.6.5 Streptocarpus hybridus ........................... 1.2.6.6 Aeschynanthus ellipticus 1 .2.7 The pest..... ......... .. .. .45 1.2.7.1 Planococcus citri (Risso):comonmealybug ......................45 1.2.7.2 Pseudococcus affinis (Maskell). The obscure mealybug ..•..•••••1$7 1.3 MATERIALS METHODS.......S.......................................'9 1.3.0.1 Preparation of plant materials..................................1i9 1.3.0.2 Physical Environment............................................119 1.3.0.3 Method of obtaining crawlers....................................50 1 .3.Q.i4 Incubation of mealybugs eggs.................... ................50 1.3.1 Influence of host plant on the developmental period of mealybug. ........... .51 1 .3.2 Influence of the host plant on the pre-ovipositional period of mealybugs.............................................52 1.3.3 Influence of plant nutrient on mealybug fecundity...............52 1 .3.LL Influence of host plant on mealybug egg viability...............53 1 .3.5 Influence of host plant on mealybug egg incubation period........ ........ .............. ........... .. 1 .3.6 Influence of host plant on mealybug body weight.. .... .,.. 51$ 1 .3.7 Correlation between weight, length and fecundity of mealybug.....................................................511 1 .3.8 Influence of host plants on the growth parameters of mealybug. .. .. .55 1.3.9 Analysisofhostplantsphenolics...............................55 1.3.10 Analysis of host plants total nitrogen content..................56 9 CONTENTS (cont.) 1 •14 RESULTS. .57 1.11.1 Influence of host plant on the developmental period of 1.14.2 Influence of host plant on the pre-ovipositiorial period of 1.11.3 Influence of host plant on mealybug fecundity.,................60 1 .11.14 Infuence of host plant on mealybug egg viability. ....... .. .61 1 . Il .5 Influence of host plants on mealybug egg incubation period. .62 1 .14.6 Influence of host plant on mealybug body weight.. ....... .. .63 1.11.7 Correlation between weight, length and fecundity of mea.lybug. .614 1 .11.8 Influence of host plant on the growth parameters of mealybug. .70 1.11.9 Analysis of host plant soluble phenois..........................71 1.14.10 Analysis of host plant total nitrogen content..............,....75 1 .5 DISCUSSION. .79 1.5.1 Influence of host plant on the developmental period of 1 .5.2 Influence of host plants on the pre-ovipositional period of ........82 1.5.3 Influence of host plant on mealybug fecundity...................83 1.5.11 Influence of host plants on mealybug egg viability..,...........85 1.5.5 Influence of host plant on mealybug egg incubation period. .85 1.5.6 Influence of host plant on mealybug body weight...............85 CONTENTS (cont.) 1 .5.7 Correlation between weight, length and fecundity of aealybugs. ............ 86 1.5.8 Influence of host plant on the growth parameters of inealybug. 87 1 .6 CONCLUSIONS.. • • •• .........• • • ,,•••••, • . • . • •....•.•. •... .90 SECTION 2 EFFECT OF HOST PLANT FACTORS ON MEALYBUG PARASITOIDS 2.1