DNA Barcoding of Six Ethnomedicinal and Economically Important Pandanus Parkinson (Pandanaceae) in the Philippines
Philippine Journal of Science 150 (5): 1321-1335, October 2021 ISSN 0031 - 7683 Date Received: 15 Mar 2021 DNA Barcoding of Six Ethnomedicinal and Economically Important Pandanus Parkinson (Pandanaceae) in the Philippines Propa Joy R. Santor1,2*, Jorge Anton D. Ordas1,2, Jay Edneil C. Olivar2, Porferio S. Bangcaya3, Maribel G. Nonato2,4, and Grecebio Jonathan D. Alejandro1,2,4 1Department of Biological Sciences, College of Science University of Santo Tomas, España, Manila, Philippines 2Research Center for the Natural and Applied Sciences University of Santo Tomas, España, Manila, Philippines 3University of Antique–Tario Lim Memorial Campus Poblacion, Tibiao, Antique, Philippines 4The Graduate School University of Santo Tomas, España, Manila, Philippines Members of the genus Pandanus are popularly known for their variety of uses, yet there is difficulty identifying its species since discrimination is dependent on its seasonal reproductive parts. DNA barcoding was utilized to establish an ideal locus for the rapid identification and authentication of six Pandanus species in the Philippines. A total of 17 samples representing six species with three endemic taxa were collected and analyzed. Genomic DNA was extracted from leaf samples and was used as a template to generate the DNA barcodes. The BLAST (Basic Local Alignment Search Tool) method, divergence, and NJ (neighbor-joining) analyses were conducted to determine the efficiency of each barcode. The internal transcribed spacer, a nuclear region, was initially assessed in this study; however, plastid markers showed more universality with better amplification and sequencing success rates. In terms of discriminatory power, trnH-psbA revealed a significantly higher interspecific divergence compared to its intraspecific divergence.
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