Anti-DDX47 Antibody

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D224769 Anti-DDX47 antibody Cat. No. D224769 Package 25 μl/100 μl/200 μl Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Product overview Description A n ti-DDX47 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human DDX47 Reactivity Human, Mouse Content 0 .6 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification Target information Symbol DDX47 Full name DEAD-box helicase 47 Synonyms RRP3; E4-DBP; HQ0256; MSTP162 Swissprot Q9H0S4 Target Background This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variantsS encodingangon distinct isoforms have been found for Bithis gene. otech Applications Immunohistochemistry Predicted cell location: Nucleus Positive control: Human esophagus cancer Recommended dilution: 50-200 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using D224769(DDX47 Antibody) at dilution 1/80, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:51 kDa Positive control:A172 cell lysate Recommended dilution: 500-2000 Gel: 8%SDS-PAGE Lysate: 40 μg Lane: A172 cell lysate Primary antibody: D224769(DDX47 Antibody) at dilution 1/600 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 1 minute ELISA Sangon Biotech Recommended dilution: 5000-10000 .

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Integrating Single-Step GWAS and Bipartite Networks Reconstruction Provides Novel Insights Into Yearling Weight and Carcass Traits in Hanwoo Beef Cattle
animals Article Integrating Single-Step GWAS and Bipartite Networks Reconstruction Provides Novel Insights into Yearling Weight and Carcass Traits in Hanwoo Beef Cattle Masoumeh Naserkheil 1 , Abolfazl Bahrami 1 , Deukhwan Lee 2,* and Hossein Mehrban 3 1 Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj 77871-31587, Iran; [email protected] (M.N.); [email protected] (A.B.) 2 Department of Animal Life and Environment Sciences, Hankyong National University, Jungang-ro 327, Anseong-si, Gyeonggi-do 17579, Korea 3 Department of Animal Science, Shahrekord University, Shahrekord 88186-34141, Iran; [email protected] * Correspondence: [email protected]; Tel.: +82-31-670-5091 Received: 25 August 2020; Accepted: 6 October 2020; Published: 9 October 2020 Simple Summary: Hanwoo is an indigenous cattle breed in Korea and popular for meat production owing to its rapid growth and high-quality meat. Its yearling weight and carcass traits (backfat thickness, carcass weight, eye muscle area, and marbling score) are economically important for the selection of young and proven bulls. In recent decades, the advent of high throughput genotyping technologies has made it possible to perform genome-wide association studies (GWAS) for the detection of genomic regions associated with traits of economic interest in diﬀerent species. In this study, we conducted a weighted single-step genome-wide association study which combines all genotypes, phenotypes and pedigree data in one step (ssGBLUP). It allows for the use of all SNPs simultaneously along with all phenotypes from genotyped and ungenotyped animals. Our results revealed 33 relevant genomic regions related to the traits of interest.
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DDX47 Polyclonal Antibody
PRODUCT DATA SHEET Bioworld Technology,Inc. DDX47 polyclonal antibody Catalog: BS71653 Host: Rabbit Reactivity: Human,Mouse,Rat BackGround: munogen and the purity is > 95% (by SDS-PAGE). This gene encodes a member of the DEAD box protein Applications: family. DEAD box proteins, characterized by the con- WB 1:500 - 1:2000 served motif Asp-Glu-Ala-Asp (DEAD), are putative IHC 1:50 - 1:200 RNA helicases. They are implicated in a number of cellu- Storage&Stability: lar processes involving alteration of RNA secondary Store at 4°C short term. Aliquot and store at -20°C long structure, such as translation initiation, nuclear and mito- term. Avoid freeze-thaw cycles. chondrial splicing, and ribosome and spliceosome assem- Specificity: bly. Based on their distribution patterns, some members DDX47 polyclonal antibody detects endogenous levels of of this family are believed to be involved in embryogene- DDX47 protein. sis, spermatogenesis, and cellular growth and division. DATA: The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript var- iants encoding distinct isoforms have been found for this gene. Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Western blot analysis of extracts of various cell lines, using DDX47 an- Molecular Weight: tibody. ~ 50 kDa Note: Swiss-Prot: For research use only, not for use in diagnostic procedure. Q9H0S4 Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific im- Bioworld Technology, Inc. Bioworld technology, co. Ltd.
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An Adipocyte-Specific Lncrap2 - Igf2bp2 Complex Enhances Adipogenesis and Energy Expenditure by Stabilizing Target Mrnas
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Primepcr™Assay Validation Report
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